CN109700687A

CN109700687A - Flexible lipidosome cosmetics comprising active peptides and preparation method thereof

Info

Publication number: CN109700687A
Application number: CN201910094274.4A
Authority: CN
Inventors: 杨莉; 魏于全
Original assignee: Shenzhen Noble Kemei Biotechnology Co Ltd
Current assignee: Shenzhen Noble Kemei Biotechnology Co Ltd
Priority date: 2018-02-13
Filing date: 2019-01-30
Publication date: 2019-05-03
Anticipated expiration: 2039-01-30
Also published as: CN109700687B

Abstract

The invention belongs to cosmetic fields, and in particular to a kind of flexible lipidosome cosmetics and preparation method thereof comprising active peptides.Due to the barrier action of skin epidermis cuticula, active constituent in cosmetics containing active peptides is difficult to mostly through skin, the present invention, aiming to the above problems, provides a kind of flexible lipidosome cosmetics comprising active peptides, it include that cosmetics are made in active peptides by the flexible lipidosome modified by hydrophobization modified polypeptide, effectively raise the transmitance of active peptides in cosmetics, it develops one kind and is easier to absorption, anti-oxidant, the better cosmetics of anti-aging effects, have a extensive future.

Description

Flexible lipidosome cosmetics comprising active peptides and preparation method thereof

Technical field

The invention belongs to cosmetic fields, and in particular to a kind of flexible lipidosome cosmetics and its system comprising active peptides Preparation Method.

Background technique

Polypeptide is a kind of compound by two or more amino acid condensations and connected with peptide bond, it is protein Intermediate material.A kind of amino acid that peptide the contains thus referred to as oligopeptides less than 10, be more than is known as polypeptide, and amino acid is more than 50 A above polypeptide is known as protein.The bioactivity of polypeptides matter is high, it is adjustable the various physiological activities of body cell And biochemical reaction, also it is referred to as active bio polypeptide.

In the defence and nursing process of skin natural aging, active bio polypeptide also plays unique and important physiology and makees With, as the proliferation of skin tissue cell, cell chemotaxis and migration, reparation and regeneration, vascularization and reconstruction, pigment formed with it is clear Remove and albumen synthesis with secretion, metabolism with regulation etc..

Current widely used active peptides relevant to beautifying skin are some chemically synthesized, molecular weight mostly Lesser functional polypeptide --- victory peptides (cosmetics peptide, CP) can be divided into three categories according to function: 1) being resisted Wrinkle class, can block the signal of face nerve transmitting contraction of muscle, and influence leather bag nerve conduction, make facial muscle relaxation, dynamically, Static wrinkle and facial lines are improved, including Argireline (also known as Argireline, argireline), palmityl Tripeptides, acetyl group octapeptide etc.；2) free radical resisting class, it is anti-inflammatory and anti-oxidant to facilitate skin, including palmityl tetrapeptide -7, gluathione Peptide, carnosine, N-BETA-Alanyl-L-histidine etc.；3) promote cell activity class, promote the conjunction of collagen and Glucosamine in human fibroblast At reinforcing connective tissue increases the skin degree of packing, promotes cell activity, including Matrixyl -3, Matrixyl -4, copper Win peptide, palmityl tripeptides -1, palmityl tripeptides -5, acetyl group tetrapeptide -9, myristoyl hexapeptide -4 etc.；4) whitening, detumescence, rush eyelash Other classes such as hair growth inhibit to promote melanocyte melanin generation, or mitigate eye edema or human activin as hygroscopic agent Keratin gene is expressed to promote eyelashes to grow, including nonapeptide -1, dipeptides -2, acetyl group tetrapeptide -5, myristoyl pentapeptide -17. Deng.

The molecular weight of these active peptides has been much less compared with albumen, some have also carried out hydrophobic modification, but by In the barrier action of skin epidermis cuticula, most active constituent is difficult to pass through.Therefore, developing can be by active peptides There is class etc. the active material of anti-oxidant, anti-aging effects etc. to carry out the cosmetics of efficient transdermal delivery, be of great significance.

Summary of the invention

The purpose of the present invention is to provide a kind of cosmetics of flexible lipidosome comprising active peptides.

The cosmetics of flexible lipidosome comprising active peptides of the invention are by the peptide modified soft of hydrophobization modification Property liposome include active peptides made of.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the hydrophobization is modified more Peptide is the polypeptide of nitrogen end hydrophobization modification.

Wherein, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the hydrophobization is modified peptide modified Flexible lipidosome be made of the raw material of following main component: by weight, lecithin: NaTDC or polysorbate 80 or polysorbate80 derivative=7:2-4, the hydrophobization modified polypeptide for being 1-10% containing weight percent, also containing work Property polypeptide.

Wherein, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, also contain antioxidant.The antioxygen Agent includes: at least one of vitamin C or derivatives thereof, vitamin E or derivatives thereof or ubiquinone.The antioxidant Weight percent be 0.1-1%.

Wherein, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the active peptides are that can make up The active peptides that product field uses.It is described can the active peptides that cosmetic field uses be with crease-resistant class, free radical resisting class, Promote cell activity class, whitening class, detumescence class or the active peptides for promoting at least one of eyelashes growth class function.

Specifically include copper victory peptide, dipeptides -2, palmityl tripeptides, palmityl tripeptides -1, palmityl tripeptides -5, palmityl four Peptide -7, acetyl group tetrapeptide -5, acetyl group tetrapeptide -9, Matrixyl -3, Matrixyl -4, Argireline, acetyl group At least one of octapeptide, myristoyl pentapeptide -17, myristoyl hexapeptide -4, nonapeptide -1, carnosine, N-BETA-Alanyl-L-histidine or glutathione.

Further, the weight percent of the active peptides is 0.01~1%.

Specifically, the composition of the polypeptide is as follows:

Argireline: Ac-Glu-Glu-Met-Gln-Arg-Arg-NH₂；

Acetyl group tetrapeptide -5:Ac-beta-Ala-His-Ser-His-OH；

Matrixyl -4:PAL (palmitinic acid)-Lys-Thr-Thr-Lys-Ser-OH；

Palmityl tetrapeptide -7:PAL (palmitinic acid)-Gly-Gln-Pro-Arg-OH；

Palmityl tripeptides -1:PAL (palmitinic acid)-Gly-His-Lys-OH；

Myristoyl pentapeptide -17:Myr-Lys-Leu-Ala-Lys-Lys-NH₂；

Carnosine: beta-Ala-L-His；

Glutathione: NH2-Glu-Cys-Gly-OH；

Nonapeptide -1:Met-Pro-D-Phe-Arg-D-Trp-Phe-Lys-Pro-Val-NH₂。

The amino acid sequence of SEQ ID NO:2 nonapeptide -1

MPFRWFKPV。

Further, it in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, is prepared by the ingredient of following proportions Form: lecithin: NaTDC or polysorbate80 or polysorbate80 derivative=7:3 contain weight percent For 0.5% antioxidant, the hydrophobization modified polypeptide for being also 2-10% containing weight percent.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, in the hydrophobization modified polypeptide The sequence of polypeptide is to connect NH in C-terminal on the basis of SEQ ID NO:1₂, and in the nitrogen end conjugated sterols class chemical combination of polypeptide Object or saturated straight chain fatty acid.

The amino acid sequence of SEQ ID NO:1 hydrophobization modified polypeptide

VQWRIRVAVIRK。

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the phytosterin compound is Cholesterol compound or Cholic acids compound.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the phytosterin compound is At least one of cholesterol, succinylated cholesterol, cholic acid or deoxycholic aicd.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the saturated straight chain fatty acid is At least one of C6~C20.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the saturated straight chain fatty acid is At least one of C8~C18.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the long chain fatty acids are hard At least one of resin acid, palmitic acid, lauric acid or caprylic acid.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the hydrophobization modified polypeptide knot Structure are as follows:

Its In, the R is phytosterin compound or saturated straight chain fatty acid.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the R is

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the crease-resistant class active peptides For at least one of palmityl tripeptides, Argireline or acetyl group octapeptide.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the crease-resistant class polypeptide accounts for rouge Plastid body weight percentage is 0.01~3%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the crease-resistant class polypeptide accounts for rouge Plastid weight percent is 0.05~2%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the crease-resistant class polypeptide accounts for rouge The weight percent of plastid is 0.1~1%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the free radical resisting class polypeptide For at least one of glutathione, palmityl tetrapeptide -7, carnosine or N-BETA-Alanyl-L-histidine.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the free radical resisting class polypeptide Accounting for Via Liposomes weight percent is 0.01~3%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the free radical resisting class polypeptide Accounting for liposome weight percent is 0.05~2%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the free radical resisting class polypeptide The weight percent for accounting for liposome is 0.1~1%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the promotion cell activity class Active peptides are Matrixyl -3, Matrixyl -4, copper victory peptide, palmityl tripeptides -1, palmityl tripeptides -5, acetyl group four At least one of peptide -9 or myristoyl hexapeptide -4.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the promotion cell activity class It is 0.01~3% that active peptides, which account for Via Liposomes weight percent,.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the promotion cell activity class It is 0.05~2% that active peptides, which account for liposome weight percent,.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the promotion cell activity class The weight percent that active peptides account for liposome is 0.1~1%.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the whitening class active peptides For nonapeptide -1.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the detumescence class active peptides For acetyl group tetrapeptide -5 or dipeptides -2.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, the rush eyelashes growth class is living Property polypeptide be myristoyl pentapeptide -17.

Further, the above-mentioned flexible lipidosome cosmetics comprising active peptides, the whitening, promote ciliogensis at detumescence It is 0.01~3% that long class active peptides, which account for Via Liposomes weight percent,.

Further, the above-mentioned flexible lipidosome cosmetics comprising active peptides, the whitening, promote ciliogensis at detumescence It is 0.05~2% that long class active peptides, which account for Via Liposomes weight percent,.

Further, the above-mentioned flexible lipidosome cosmetics comprising active peptides, the whitening, promote ciliogensis at detumescence It is 0.1~1% that long class active peptides, which account for Via Liposomes weight percent,.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, described includes active peptides Flexible lipidosome cosmetics further include have emulsifier related with cosmetics are prepared, assistant for emulsifying agent, skin conditioning agent, brightening agent, Colorant, moisturizer, solubilizer, surfactant, preservative, aromatic, emollient, anti-acne agent, film forming agent, thickener, At least one of pH adjusting agent, buffer, stabilizer or ultraviolet absorbing agent.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, described includes active peptides The dosage form of flexible lipidosome cosmetics is aqua, emulsion, paste, gelatin dose, ingot shape dosage form or vapour colloidal sol type dosage form.

Further, in the cosmetics of the above-mentioned flexible lipidosome comprising active peptides, described includes active peptides The dosage form of flexible lipidosome cosmetics is lotion, Essence, face cream, facial mask, freeze-dried powder or facial cleanser.

The present invention also provides a kind of methods for preparing the above-mentioned flexible lipidosome cosmetics comprising active peptides, including with Lower step:

A, lecithin, NaTDC or polysorbate80 are weighed or polysorbate80 derivative, vitamin E are placed in In reaction vessel, solvent dissolution is added, the solvent is the mixture or all ethyl alcohol that chloroform and ethyl alcohol press 1: 1-3 composition；

B, rotary evaporation in vacuo obtains liposome membrane, dry；

C, DP7-C or PAL-DP7 and distilled water are added in liposome membrane, it is molten that the ultrasonic aquation of progress obtains blank liposome Liquid；

D, active peptides are added in blank liposomes liquid solution, it is slight to shake, be incubated at room temperature 30-60 minutes to get comprising The flexible lipidosome of active peptides.

Wherein, above-mentioned preparation includes in the method for the flexible lipidosome cosmetics of active peptides, further includes step e: will be true Liposome after sky decompression is pressed through 0.1-0.45 μm of polycarbonate membrane 4~8 times, obtains blank liposome solutions.

Wherein, above-mentioned preparation includes in the method for the flexible lipidosome cosmetics of active peptides, further includes step f: by agent Type requirement, flexible lipidosome is added again prepare the related emulsifier of cosmetics, assistant for emulsifying agent, skin conditioning agent, brightening agent, Toner, moisturizer, solubilizer, surfactant, preservative, aromatic, emollient, anti-acne agent, film forming agent, thickener, pH State of cosmetics is made at least one of regulator, buffer, stabilizer or ultraviolet absorbing agent.

Cosmetics of the present invention refer to embrocate, spray or other similar method, it is any to intersperse among human body surface Position (skin, hair, nail, lip etc.), to reach cleaning, eliminate bad smell, skin care, beauty and modification purpose dailyization Learn industrial products.

Flexible lipidosome is a kind of self aggregation vesicle on the basis of liposome through recipe improvement, liposome phosphatide at Surface reactive material such as sodium taurocholate, polysorbate80 or derivatives thereof etc. is added in point, makes its lipid membrane that there is high deformation Ability, also known as carrier.Since carrier partial size is smaller than liposome, can pass through aperture be itself 1/10~1/5 aperture, Transmission rates and amount are almost suitable with pure water.

The mechanism of action of flexible lipidosome (carrier) of the invention are as follows: 1, carrier partial size it is smaller compared with liposome, can be fast Speed penetrates keratoderma, into epidermis and corium, is formed " storage cavern "；2, the intracorporal skin actives of transmitting and water are encapsulated in Dividing can slowly release, and greatly improve the function and effect of active material.3, the carrier carries encapsulated skin activity Polypeptide, such as palmityl tripeptides or acetyl group hexapeptide -3 can block the signal of face nerve transmitting contraction of muscle, play crease-resistant work With；Or Matrixyl -3, palmityl tripeptides -1 or acetyl group tetrapeptide -9 etc. can promote in human fibroblast collagen and Connective tissue is strengthened in the synthesis of Glucosamine, plays the role of repairing skin；Or palmityl tetrapeptide -7, carnosine etc. can resist certainly By base, play the role of anti-inflammatory and oxidation resistant；Or nonapeptide -1 etc. inhibits melanocyte to generate melanin, plays the work of whitening With；Or acetyl group tetrapeptide -5, dipeptides -2 etc. eliminate oedema, play the role of eye pouch of dispelling；Or myristoyl pentapeptide -17 etc. activates people The expression of body keratin gene promotes eyelashes growth.

Further, the present invention is equipped with emulsifier related with cosmetic formulations containing the carrier of active peptides, helped Emulsifier, skin conditioning agent, brightening agent, colorant, moisturizer, solubilizer, surfactant, preservative, aromatic, moisturizing Agent, anti-acne agent, film forming agent, thickener, pH adjusting agent, buffer, stabilizer, ultraviolet absorbing agent etc., according to this field Technology can be prepared into the cosmetics of the dosage forms such as aqua, lotion, Essence, face cream, facial mask, freeze-dried powder, facial cleanser.The present invention Various products can be used for human body face (including eye), neck, trick and whole skin.

The invention has the benefit that

The present invention provides a kind of novel flexible liposome through recipe improvement on the basis of traditional liposome, by rouge Surface reactive material such as NaTDC, polysorbate80 or derivatives thereof etc. is added in the phospholipid composition of plastid, makes its class Adipose membrane has high deformation ability, while the cell-penetrating peptide that can reinforce transdermal effect is added, and improves its transdermal penetration ability.Inventor It is used for above-mentioned carrier to wrap up active peptides, using its excellent transdermal capability, is prepared into novel active polypeptide transdermal agent, and Develop its application in cosmetic field.Novel active polypeptide transdermal agent material therefor of the present invention is safe and non-toxic, crease-resistant, anti- The good effect of aging, anti-oxidant, reparation etc. display.In addition, it has the function of biomembrane and characteristic, with human body cell There is very strong compatibility, it is unique the effect of in terms of cosmetics, there is huge economic benefit.

Preliminary test is shown: the carrier carries encapsulated active peptides, such as palmityl tripeptides or acetyl group hexapeptide- 3 etc. can block the signal of face nerve transmitting contraction of muscle, play the role of crease-resistant；Encapsulate Matrixyl -3, palmityl tripeptides - 1 or acetyl group tetrapeptide -9 etc. can promote the synthesis of collagen and Glucosamine in human fibroblast, strengthen connective tissue, Play the role of repairing skin；Encapsulate palmityl tetrapeptide -7, carnosine etc. can free radical resisting, play the role of anti-inflammatory and oxidation resistant； Or nonapeptide -1 etc. inhibits melanocyte to generate melanin, plays the role of whitening；Or acetyl group tetrapeptide -5, dipeptides -2 etc. are eliminated Oedema plays the role of eye pouch of dispelling；Or the equal human activins keratin gene expression of myristoyl pentapeptide -17, promote eyelashes growth.

In technical level, the invention has the advantages that: product of the present invention, which is removed, has general anti-sad old in the market, moisturizing Profit promotees outside soft function, which is also applied to cosmetics for novel percutaneous technique, has better effect.

Detailed description of the invention

Fig. 1 is that modifying using polysorbate80 as the DP7-C of the acetyl-containing hexapeptide -8 of surfactant for preparation is flexible Liposome nano granule diameter detection figure；

Fig. 2 is the soft as the PAL-DP7 of the acetyl-containing hexapeptide -8 of surfactant modification using polysorbate80 of preparation Property liposome nano granule diameter detection figure；

Fig. 3 is that the DP7-C using polysorbate80 as surfactant tetrapeptide containing palmityl -7 of preparation modifies flexible rouge Plastid nanometer particle size detection figure；

Fig. 4 is that the PAL-DP7 modification using polysorbate80 as surfactant tetrapeptide containing palmityl -7 of preparation is flexible Liposome nano granule diameter detection figure；

Fig. 5 is that the DP7-C by surfactant of polysorbate80 containing Matrixyl -4 of preparation modifies flexible rouge Plastid nanometer particle size detection figure；

Fig. 6 is that the PAL-DP7 modification by surfactant of polysorbate80 containing Matrixyl -4 of preparation is flexible Liposome nano granule diameter detection figure；

Fig. 7 is the PAL-DP7 modification flexible lipidosome by surfactant of polysorbate80 containing nonapeptide -1 of preparation Nanometer particle size detection figure；

Fig. 8 is that the DP7-C modification flexible lipidosome by surfactant of polysorbate80 containing nonapeptide -1 of preparation is received Rice droplet measurement figure；

Fig. 9 is that the DP7-C using polysorbate80 as surfactant acetyl-containing tetrapeptide -5 of preparation modifies flexible rouge Plastid nanometer particle size detection figure；

Figure 10 is that the PAL-DP7 modification using polysorbate80 as surfactant acetyl-containing tetrapeptide -5 of preparation is flexible Liposome nano granule diameter detection figure；

Figure 11 be preparation by surfactant pentapeptide containing myristoyl -17 of polysorbate80=DP7-C modification Flexible lipidosome nanometer particle size detection figure；

Figure 12 is the PAL-DP7 modification using polysorbate80 as surfactant pentapeptide containing myristoyl -17 of preparation Flexible lipidosome nanometer particle size detection figure；

Figure 13 is the transdermal test in vitro effect of DP7-C and PAL-DP7 the modification flexible lipidosome of the acetyl-containing hexapeptide -8 of preparation Fruit；

Figure 14 is the transdermal test in vitro effect of DP7-C and PAL-DP7 the modification flexible lipidosome of the tetrapeptide containing palmityl -7 of preparation Fruit；

Figure 15 is the transdermal test in vitro effect of the DP7-C and PAL-DP7 modification flexible lipidosome containing Matrixyl -4 of preparation Fruit；

Figure 16 is the transdermal test in vitro effect of the DP7-C and PAL-DP7 modification flexible lipidosome containing nonapeptide -1 of preparation；

Figure 17 is the transdermal test in vitro effect of DP7-C and PAL-DP7 the modification flexible lipidosome of the acetyl-containing tetrapeptide -5 of preparation Fruit；

Figure 18 is the transdermal test in vitro of DP7-C and PAL-DP7 the modification flexible lipidosome of the pentapeptide containing myristoyl -17 of preparation Effect.

Specific embodiment

Heretofore described hydrophobization modified polypeptide DP7-C is the conjugate of cecropin D P7 and cholesterol, and hydrophobization is repaired The conjugate that polypeptide PAL-DP7 is cecropin D P7 and palmitic acid is adornd, synthetic method is referring to the conjunction in patent CN107441501A At method, other methods that this field can also be used are synthesized, and DP7-C used in embodiment and PAL-DP7 are triumphant by Chengdu The synthesis of prompt biological medicine development in science and technology Co., Ltd.

Raw material, equipment used in the specific embodiment of the invention are known product, are obtained by purchase commercial product.

The preparation of the flexible lipidosome of the invention containing active peptides of embodiment 1

By formula accurate weighing soybean lecithin, NaTDC or polysorbate80, vitamin E, respectively with chloroform/ Ethyl alcohol or ethyl alcohol dissolution, dissolve mixing, rotary evaporation 2 hours, are placed in vacuum for gained film at room temperature in 250ml pears type bottle In drying box overnight.Second day addition distilled water, appropriate hydrophobization modified polypeptide, 400w Probe Ultrasonic Searching 30min obtain blank transmitting Liquid solution.It is slow added into appropriate acetyl group octapeptide -8 or palmityl tetrapeptide or Matrixyl or nonapeptide -1 or acetyl group four Peptide -5 or myristoyl pentapeptide -17 etc., incubation at room temperature after forty minutes, by the mother liquid obtained multiple mistake of the polycarbonate membrane with 0.2 μm Filter is added 5% mannitol of excipient, is sub-packed in cillin bottle, freeze-dried rear up to the flexible lipid of the invention containing active peptides Body.

Test example 1-1 includes the preparation of the DP7-C modification flexible lipidosome of Argireline

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

NaTDC: it is purchased from the extensive and profound in meaning star biotechnology responsibility Co., Ltd in Beijing；

Polysorbate80: it is purchased from U.S. Merck company；

Hydrophobization modified polypeptide DP7-C: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Argireline: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising Argireline are as follows: soybean The ratio of lecithin and NaTDC or soybean lecithin and polysorbate80 is 8.7:1.3, hydrophobization modified polypeptide DP7-C ratio is 55%, and the final concentration of 50ppm of Argireline, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Argireline is slow added into end Concentration is 50ppm, and after incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, and excipient is added 5% mannitol, is sub-packed in cillin bottle, and the acetyl-containing using NaTDC as surfactant is respectively obtained after freeze-dried The flexible lipidosome 1-1 of hexapeptide -8 and using polysorbate80 as the flexible lipidosome of the acetyl-containing hexapeptide -8 of surfactant 1-2, partial size are respectively 74.47nm and 68.57nm or so, are shown in Table 1, are distinguished using the encapsulation rate that supercentrifugation measures product For 45.6% and 52.65%.Wherein, the flexible lipidosome modified using polysorbate80 as surfactant, DP7-C, grain Diameter figure is shown in Fig. 1.

Test example 1-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of Argireline

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Hydrophobization modified polypeptide PAL-DP7: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising Argireline are as follows: big Beans lecithin and NaTDC or soybean lecithin and polysorbate80 ratio are 8.5:1.5, hydrophobization modified polypeptide PAL-DP7 ratio is 6%, and the final concentration of 50ppm of Argireline, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80 Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.It is slow added into Argireline extremely After incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times by final concentration of 50ppm, and figuration is added 5% mannitol of agent, is sub-packed in cillin bottle, and the acetyl-containing using NaTDC as surfactant is respectively obtained after freeze-dried The flexible lipidosome 1-3 of hexapeptide -8 and using polysorbate80 as the flexible lipidosome of the acetyl-containing hexapeptide -8 of surfactant 1-4, partial size are respectively 70.34nm and 59.23nm or so, are shown in Table 1, are distinguished using the encapsulation rate that supercentrifugation measures product For 47.2% and 55.17%.Wherein nanometer particle size is minimum, encapsulation rate it is best be using polysorbate80 as surfactant, The flexible lipidosome of PAL-DP7 modification, grain-size graph are shown in Fig. 2.

Table 1 prepares the nanometer characterization situation of the different composition flexible lipidosomes of acetyl-containing hexapeptide -8

Test example 2 includes the preparation of the flexible lipidosome of palmityl tetrapeptide -7

According to above Research Thinking, while being prepared for the flexible lipidosome of palm tetrapeptide -7.

Test example 2-1 includes the preparation of the DP7-C modification flexible lipidosome of palmityl tetrapeptide -7

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Palmityl tetrapeptide -7: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising palmityl tetrapeptide -7 are as follows: soybean The ratio of lecithin and NaTDC or soybean lecithin and polysorbate80 is 8:2, hydrophobization modified polypeptide DP7-C Ratio is 5%, the final concentration of 5ppm of palmityl tetrapeptide -7, and aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Palmityl tetrapeptide -7 is slow added into end Concentration is 5ppm, and after incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, and excipient is added 5% mannitol, is sub-packed in cillin bottle, and being respectively obtained after freeze-dried using NaTDC is surfactant containing palmityl The flexible lipidosome 2-1 of tetrapeptide -7 and using polysorbate80 as the flexible lipidosome of the tetrapeptide containing palmityl -7 of surfactant 2-2, partial size are respectively 82.69nm and 79.47nm or so, are shown in Table 2, are distinguished using the encapsulation rate that supercentrifugation measures product For 47.65% and 48.1%.Wherein, the flexible lipidosome modified using polysorbate80 as surfactant, DP7-C, grain Diameter figure is shown in Fig. 3.

Test example 2-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of palmityl tetrapeptide -7

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising palmityl tetrapeptide -7 are as follows: big The ratio of beans lecithin and NaTDC or soybean lecithin and polysorbate80 is 8.5:1.5, and hydrophobization modification is more Peptide PAL-DP7 ratio is 6%, and the final concentration of 5ppm of palmityl tetrapeptide -7, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Be slow added into palmityl tetrapeptide -7 to After incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times by final concentration of 5ppm, and figuration is added 5% mannitol of agent, is sub-packed in cillin bottle, and being respectively obtained after freeze-dried using NaTDC is surfactant containing palm The flexible lipidosome 2-3 of acyl tetrapeptide -7 and using polysorbate80 as the flexible lipid of the tetrapeptide containing palmityl -7 of surfactant Body 2-4, partial size are respectively 75.07nm and 72.57nm or so, are shown in Table 2, and the encapsulation rate point of product is measured using supercentrifugation It Wei 48.1% and 49.27%.Wherein nanometer particle size minimum, best encapsulation rate are using polysorbate80 as surface-active The flexible lipidosome of agent, PAL-DP7 modification, grain-size graph are shown in Fig. 4.

The nanometer that table 2 prepares the different composition flexible lipidosomes of tetrapeptide containing palmityl -7 characterizes situation

Test example 3 includes the preparation of the flexible lipidosome of Matrixyl -4

According to above Research Thinking, while being prepared for the flexible lipidosome of palm pentapeptide -4.

Test example 3-1 includes the preparation of the DP7-C modification flexible lipidosome of Matrixyl -4

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising Matrixyl -4 are as follows: soybean The ratio of lecithin and NaTDC or soybean lecithin and polysorbate80 is 8.5:1.5, hydrophobization modified polypeptide DP7-C ratio is 5%, and the final concentration of 10ppm of Matrixyl -4, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E is dissolved with ethyl alcohol respectively, mixing is dissolved in 250ml pears type bottle, at room temperature rotary evaporation 2 hours, by gained film It is placed in a vacuum drying oven overnight.Second day addition distilled water, appropriate hydrophobization modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Matrixyl -4 is slow added into final concentration of 10ppm, incubation at room temperature 30 minutes Afterwards, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, 5% mannitol of excipient is added, is sub-packed in cillin bottle, Respectively obtained after freeze-dried using NaTDC be surfactant containing Matrixyl -4 flexible lipidosome 3-1 and with Polysorbate80 be surfactant the flexible lipidosome 3-2 containing Matrixyl -4, partial size be respectively 89.37nm and 82.28nm or so is shown in Table 3, is respectively 44.3% and 47.12% using the encapsulation rate that supercentrifugation measures product.Wherein, with Polysorbate80 is the flexible lipidosome of surfactant, DP7-C modification, and grain-size graph is shown in Fig. 5.

Test example 3-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of Matrixyl -4

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising Matrixyl -4 are as follows: big The ratio of beans lecithin and NaTDC or soybean lecithin and polysorbate80 is 8:2, hydrophobization modified polypeptide PAL-DP7 ratio is 6%, and the final concentration of 10ppm of Matrixyl -4, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E is dissolved with ethyl alcohol respectively, mixing is dissolved in 250ml pears type bottle, at room temperature rotary evaporation 2 hours, by gained film It is placed in a vacuum drying oven overnight.Second day addition distilled water, appropriate hydrophobization modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Matrixyl -4 is slow added into final concentration of 10ppm, incubation at room temperature 30 minutes Afterwards, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, 5% mannitol of excipient is added, is sub-packed in cillin bottle, Respectively obtained after freeze-dried using NaTDC be surfactant containing Matrixyl -4 flexible lipidosome 3-3 and with Polysorbate80 be surfactant the flexible lipidosome 3-4 containing Matrixyl -4, partial size be respectively 87.24nm and 79.47nm or so is shown in Table 3, is respectively 45.1% and 48.72% using the encapsulation rate that supercentrifugation measures product.Wherein receive Grain of rice diameter is minimum, best encapsulation rate is the flexible lipidosome modified using polysorbate80 as surfactant, PAL-DP7, Its grain-size graph is shown in Fig. 6.

The nanometer of different composition flexible lipidosomes of the preparation of table 3 containing Matrixyl -4 characterizes situation

Test example 4 includes the preparation of the flexible lipidosome of nonapeptide -1

According to above Research Thinking, while being prepared for the flexible lipidosome of nonapeptide -1.

Test example 4-1 includes the preparation of the DP7-C modification flexible lipidosome of nonapeptide -1

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Nonapeptide -1: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising nonapeptide -1 are as follows: soybean lecithin Ratio with NaTDC or soybean lecithin and polysorbate80 is 8.2:1.8, hydrophobization modified polypeptide DP7-C ratio Example is 5%, and the final concentration of 50ppm of nonapeptide -1, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Nonapeptide -1 is slow added into final concentration of After incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times by 50ppm, and it is sweet that excipient 5% is added Reveal alcohol, be sub-packed in cillin bottle, respectively obtaining using NaTDC after freeze-dried is surfactant containing the soft of nonapeptide -1 Property liposome 4-1 and using polysorbate80 as the flexible lipidosome 4-2 containing nonapeptide -1 of surfactant, partial size is respectively 69.26nm and 64.94nm or so are shown in Table 4, are respectively 52.3% He using the encapsulation rate that supercentrifugation measures product 57.26%.Wherein, the flexible lipidosome modified using polysorbate80 as surfactant, DP7-C, grain-size graph are shown in Fig. 7.

Test example 4-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of nonapeptide -1

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising nonapeptide -1 are as follows: soybean lecithin The ratio of rouge and NaTDC or soybean lecithin and polysorbate80 is 8:2, hydrophobization modified polypeptide PAL-DP7 ratio Example is 6%, and the final concentration of 50ppm of nonapeptide -1, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 2 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Nonapeptide -1 is slow added into final concentration For 50ppm, after incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, excipient 5% is added Mannitol is sub-packed in cillin bottle, and freeze-dried rear respectively obtain using NaTDC is surfactant containing nonapeptide -1 Flexible lipidosome 4-3 and using polysorbate80 as the flexible lipidosome 4-4 containing nonapeptide -1 of surfactant, partial size difference For 65.4nm and 62.11nm or so, 4 are shown in Table, is respectively 54.1% He using the encapsulation rate that supercentrifugation measures product 59.34%.It is to modify by surfactant, PAL-DP7 of polysorbate80 that wherein nanometer particle size minimum, encapsulation rate are best Flexible lipidosome, grain-size graph is shown in Fig. 8.

The nanometer of different composition flexible lipidosomes of the preparation of table 4 containing nonapeptide -1 characterizes situation

Test example 5 includes the preparation of the flexible lipidosome of acetyl group tetrapeptide -5

According to above Research Thinking, while being prepared for the flexible lipidosome of acetyl group tetrapeptide -5.

Test example 5-1 includes the preparation of the DP7-C modification flexible lipidosome of acetyl group tetrapeptide -5

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Acetyl group tetrapeptide -5: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising acetyl group tetrapeptide -5 are as follows: soybean The ratio of lecithin and NaTDC or soybean lecithin and polysorbate80 is 8.5:1.5, hydrophobization modified polypeptide DP7-C ratio is 5%, and the final concentration of 100ppm of acetyl group tetrapeptide -5, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 3 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Acetyl group tetrapeptide -5 is slow added into end Concentration is 100ppm, and after incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, and figuration is added 5% mannitol of agent, is sub-packed in cillin bottle, and being respectively obtained after freeze-dried using NaTDC is surfactant containing acetyl The flexible lipidosome 5-1 of base tetrapeptide -5 and using polysorbate80 as the flexible lipid of the acetyl-containing tetrapeptide -5 of surfactant Body 5-2, partial size are respectively 77.48nm and 64.52nm or so, are shown in Table 4, and the encapsulation rate point of product is measured using supercentrifugation It Wei 51.4% and 53.6%.Wherein, the flexible lipidosome modified using polysorbate80 as surfactant, DP7-C, grain Diameter figure is shown in Fig. 9.

Test example 5-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of acetyl group tetrapeptide -5

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising acetyl group tetrapeptide -5 are as follows: big The ratio of beans lecithin and NaTDC or soybean lecithin and polysorbate80 is 8:2, hydrophobization modified polypeptide PAL-DP7 ratio is 6%, and the final concentration of 100ppm of acetyl group tetrapeptide -5, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 3 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.Be slow added into acetyl group tetrapeptide -5 to After incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times by final concentration of 100ppm, is added and is assigned 5% mannitol of shape agent, is sub-packed in cillin bottle, and being respectively obtained after freeze-dried using NaTDC is surfactant containing second The flexible lipidosome 5-3 of acyl group tetrapeptide -5 and using polysorbate80 as the flexible rouge of the acetyl-containing tetrapeptide -5 of surfactant Plastid 5-4, partial size are respectively 66.9nm and 64.12nm or so, are shown in Table 5, and the encapsulation rate of product is measured using supercentrifugation Respectively 53.2% and 56.5%.Wherein nanometer particle size minimum, best encapsulation rate are using polysorbate80 as surface-active The flexible lipidosome of agent, PAL-DP7 modification, grain-size graph are shown in Figure 10.

Table 5 prepares the nanometer characterization situation of the different composition flexible lipidosomes of acetyl-containing tetrapeptide -5

Test example 6 includes the preparation of the flexible lipidosome of myristoyl pentapeptide -17

According to above Research Thinking, while being prepared for the flexible lipidosome of myristoyl pentapeptide -17.

Test example 6-1 includes the preparation of the DP7-C modification flexible lipidosome of myristoyl pentapeptide -17

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Myristoyl pentapeptide -17: Chengdu Kaijie Biological Medicine Science and Technology Development Co., Ltd's synthesis；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example study flexible lipidosome using DP7-C modify, surface reactive material be respectively adopted NaTDC with Prepared by polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising myristoyl pentapeptide -17 are as follows: big The ratio of beans lecithin and NaTDC or soybean lecithin and polysorbate80 is 8.5:1.5, and hydrophobization modification is more Peptide D P7-C ratio is 5%, and the final concentration of 200ppm of myristoyl pentapeptide -17, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 1 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide DP7-C, 400w Probe Ultrasonic Searching 30min obtains blank carrier solution.It is slow added into myristoyl pentapeptide -17 To final concentration of 200ppm, after incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, is added 5% mannitol of excipient, is sub-packed in cillin bottle, respectively obtains containing using NaTDC as surfactant after freeze-dried The flexible lipidosome 6-1 of myristoyl pentapeptide -17 and using polysorbate80 as the pentapeptide containing myristoyl -17 of surfactant Flexible lipidosome 6-2, partial size is respectively 131.4nm and 120.4nm or so, is shown in Table 4, measures product using supercentrifugation Encapsulation rate be respectively 47.4% and 51.16%.Wherein, the flexibility modified using polysorbate80 as surfactant, DP7-C Liposome, grain-size graph are shown in Figure 11.

Test example 6-2 includes the preparation of the PAL-DP7 modification flexible lipidosome of myristoyl pentapeptide -17

Experimental material:

Soybean lecithin: it is purchased from Lipoid GmbH company；

Polysorbate80: it is purchased from U.S. Merck company；

Instrument: Malvern laser particle size detector ZEN 3600.

This test example is studied flexible lipidosome and is modified using PAL-DP7, and NaTDC is respectively adopted in surface reactive material It is prepared with polysorbate80, the parameter of the preparation process selection of the flexible lipidosome comprising myristoyl pentapeptide -17 are as follows: The ratio of soybean lecithin and NaTDC or soybean lecithin and polysorbate80 is 8:2, hydrophobization modified polypeptide PAL-DP7 ratio is 6%, and the final concentration of 200ppm of myristoyl pentapeptide -17, aqueous vehicles are water, hydration time 30 minutes.

Specific preparation process is as follows: by formula accurate weighing soybean lecithin, NaTDC or polysorbate80, Vitamin E, with chloroform and ethyl alcohol, 1: 1 mixture formed dissolves by volume respectively, and mixing is dissolved in 250ml pears type bottle, Gained film is placed in a vacuum drying oven overnight by rotary evaporation 2 hours at room temperature.It is second day addition distilled water, appropriate hydrophobic Change modified polypeptide PAL-DP7,400w Probe Ultrasonic Searching 30min obtains blank carrier solution.It is slow added into myristoyl pentapeptide- After 17 to final concentration of 200ppm, incubation at room temperature 30 minutes, the mother liquid obtained polycarbonate membrane with 0.2 μm is filtered for multiple times, is added Enter 5% mannitol of excipient, be sub-packed in cillin bottle, is respectively obtained using NaTDC as surfactant after freeze-dried The flexible lipidosome 6-3 of pentapeptide containing myristoyl -17 and using polysorbate80 as the pentapeptide containing myristoyl of surfactant - 17 flexible lipidosome 6-4, partial size are respectively 121.4nm and 114.1nm or so, are shown in Table 6, measure production using supercentrifugation The encapsulation rate of product is respectively 49.2% and 54.1%.Wherein nanometer particle size minimum, best encapsulation rate are with polysorbate80 For the flexible lipidosome that surfactant, PAL-DP7 are modified, grain-size graph is shown in Figure 12.

The nanometer that table 6 prepares the different composition flexible lipidosomes of pentapeptide containing myristoyl -17 characterizes situation

The transdermal effect verifying of the flexible lipidosome of the invention containing active peptides of embodiment 2

Percutaneous penetration is using the single chamber Franz diffusion cell improved, using mouse skin as percutaneous penetration skin, The Transdermal absorption result of the different flexible lipidosomes containing active peptides is compared, samples, passes through in different time points respectively HPLC method measures the concentration of drug in transdermal receiving liquid, calculates and accumulates transdermal amount.

Test example 2-1 includes the transdermal effect verifying of the flexible lipidosome of Argireline

1, material

Sample: the acetyl-containing six modified using polysorbate80 as surfactant, PAL-DP7 prepared by test example 1 The flexible lipidosome freeze-dried powder (1-1,1-2,1-3,1-4) of peptide -8 and what is modified without DP7-C or PAL-DP7 includes acetyl group The flexible lipidosome of hexapeptide -8 is as normal flexible control liposome；

Instrument: drug transdermal tests diffusion instrument (Shanghai Huanghai Sea medicine inspection, model RYJ-6B)；HPLC (Agilent, model 1260)；Chromatographic column: ZORBAX 300SB-C18；

Material: the mouse skin after removing.

2, method

2.1 carry out the transdermal experiment to test agent by the operation instruction of penetrating absorption diffusion instrument, while a top is arranged The blank control for only adding buffer harvests the sample in reception tank respectively at 0hr, 1hr, 2hr, 4hr, 8hr of test.

2.2 HPLC detection: ultraviolet detection wavelength: 220nm；Mobile phase: A liquid: 100%ACN+0.1%TFA；

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 7:

7 HPLC testing conditions table of table

Time (min)	A liquid	B liquid
			0	43%	57%
20	63%	37%
			20.5	43%	57%
27	43%	57%

3, result

HPLC measurement is carried out using the polypeptide of Argireline as standard items and sample respectively, according to Argireline Standard items peak area establishes standard curve, the peak area of sample to be tested is substituted into formula, calculate sample adds up transdermal amount, sees Figure 13.It is and unmodified the result shows that with the extension of time, the Argireline amount through mouse skin is stepped up Flexible lipidosome compare, DP7-C and PAL-DP7 modification flexible lipidosome all have better percutaneous abilities.

Test example 2-2 includes the transdermal effect verifying of the flexible lipidosome of palmityl tetrapeptide -7

1, material

Sample: prepared by test example 2 contains palmityl four using polysorbate80 as what surfactant, PAL-DP7 were modified The flexible lipidosome freeze-dried powder (2-1,2-2,2-3,2-4) of peptide -7 and what is modified without DP7-C or PAL-DP7 includes palmityl The flexible lipidosome of tetrapeptide -7 is as normal flexible control liposome；

Material: the mouse skin after removing.

2, method

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 8:

8 HPLC testing conditions table of table

Time (min)	A liquid	B liquid
			0	50%	50%
25	75%	25%
			25.01	100%	0
30	100%	0
			30.01	50%	50%
35	50%	50%

3, result

HPLC measurement is carried out using the polypeptide of palmityl tetrapeptide -7 as standard items and sample respectively, according to palmityl tetrapeptide -7 Standard items peak area establishes standard curve, the peak area of sample to be tested is substituted into formula, calculate sample adds up transdermal amount, sees Figure 14.The result shows that with the extension of time, the amount of palmityl tetrapeptide -7 through mouse skin is stepped up, and it is unmodified Flexible lipidosome compare, DP7-C and PAL-DP7 modification flexible lipidosome all have better percutaneous abilities.

Test example 2-3 includes the transdermal effect verifying of the flexible lipidosome of Matrixyl -4

1, material

Sample: prepared by test example 3 contains palmityl five using polysorbate80 as what surfactant, PAL-DP7 were modified The flexible lipidosome freeze-dried powder (3-1,3-2,3-3,3-4) of peptide -4 and what is modified without DP7-C or PAL-DP7 includes palmityl The flexible lipidosome of pentapeptide -4 is as normal flexible control liposome；

Material: the mouse skin after removing.

2, method

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 9:

9 HPLC testing conditions table of table

3, result

HPLC measurement is carried out using the polypeptide of Matrixyl -4 as standard items and sample respectively, according to Matrixyl -4 Standard items peak area establishes standard curve, the peak area of sample to be tested is substituted into formula, calculate sample adds up transdermal amount, sees Figure 15.The result shows that with the extension of time, the amount of Matrixyl -4 through mouse skin is stepped up, and it is unmodified Flexible lipidosome compare, DP7-C and PAL-DP7 modification flexible lipidosome all have better percutaneous abilities.

Test example 2-4 includes the transdermal effect verifying of the flexible lipidosome of nonapeptide -1

1, material

Sample: test example 4 prepare using polysorbate80 as surfactant, PAL-DP7 modify containing nonapeptide -1 Flexible lipidosome freeze-dried powder (4-1,4-2,4-3,4-4) and the flexibility comprising nonapeptide -1 modified without DP7-C or PAL-DP7 Liposome is as normal flexible control liposome；

Material: the mouse skin after removing.

2, method

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 10:

10 HPLC testing conditions table of table

3, result

HPLC measurement is carried out using the polypeptide of nonapeptide -1 as standard items and sample respectively, according to -1 standard items peak area of nonapeptide Standard curve is established, the peak area of sample to be tested is substituted into formula, calculate sample adds up transdermal amount, sees Figure 16.As a result table It is bright, with the extension of time, the amount of nonapeptide -1 through mouse skin is stepped up, compared with unmodified flexible lipidosome, The flexible lipidosome of DP7-C and PAL-DP7 modification all has better percutaneous abilities.

Test example 2-5 includes the transdermal effect verifying of the flexible lipidosome of acetyl group tetrapeptide -5

1, material

Sample: the acetyl-containing four modified using polysorbate80 as surfactant, PAL-DP7 prepared by test example 5 The flexible lipidosome freeze-dried powder (5-1,5-2,5-3,5-4) of peptide -5 and what is modified without DP7-C or PAL-DP7 includes acetyl group The flexible lipidosome of tetrapeptide -5 is as normal flexible control liposome；

Material: the mouse skin after removing.

2, method

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 11:

11 HPLC testing conditions table of table

Time (min)	A liquid	B liquid
			0	43%	57%
20	63%	37%
			20.5	43%	57%
27	43%	57%

3, result

HPLC measurement is carried out using the polypeptide of acetyl group tetrapeptide -5 as standard items and sample respectively, according to acetyl group tetrapeptide -5 Standard items peak area establishes standard curve, the peak area of sample to be tested is substituted into formula, calculate sample adds up transdermal amount, sees Figure 17.The result shows that with the extension of time, the amount of acetyl group tetrapeptide -5 through mouse skin is stepped up, and it is unmodified Flexible lipidosome compare, DP7-C and PAL-DP7 modification flexible lipidosome all have better percutaneous abilities.

Test example 2-6 includes the transdermal effect verifying of the flexible lipidosome of myristoyl pentapeptide -17

1, material

Sample: prepared by test example 6 contains myristoyl using polysorbate80 as what surfactant, PAL-DP7 were modified The flexible lipidosome freeze-dried powder (6-1,6-2,6-3,6-4) of pentapeptide -17 and what is modified without DP7-C or PAL-DP7 includes meat The flexible lipidosome of myristoyl pentapeptide -17 is as normal flexible control liposome；

Material: the mouse skin after removing.

2, method

B liquid: 100%H₂O+0.1%TFA；Experimental condition see the table below 12:

12 HPLC testing conditions table of table

Time (min)	A liquid	B liquid
			0	43%	57%
20	63%	37%
			20.5	43%	57%
27	43%	57%

3, result

HPLC measurement is carried out using the polypeptide of myristyl pentapeptide -4 as standard items and sample respectively, according to myristyl five - 4 standard items peak area of peptide establishes standard curve, and the peak area of sample to be tested is substituted into formula, calculate sample add up it is transdermal Amount, is shown in Figure 18.The result shows that with the extension of time, through mouse skin acetyl group pentapeptide -4 amount be stepped up, with without The flexible lipidosome of modification is compared, and the flexible lipidosome of DP7-C and PAL-DP7 modification all has better percutaneous abilities.

The stability study of the flexible lipidosome freeze-dried powder of the invention containing active peptides of embodiment 3

Flexible lipidosome freeze-dried powder containing active peptides prepared by embodiment 1 carries out stability study and (including accelerates examination It tests and three aspects such as long term test).Sample is placed in 37 DEG C of testing chamber for medicine stabilities and carries out accelerated tests, place 1 respectively, 2, 3,6 months, the character of freeze-dried powder, including color, content and dissolubility is measured, the results are shown in Table 13.Sample is placed in 25 DEG C of medicines again Product stability test case carries out long term test, places 3,6,9,12,15,18,21,24 months respectively, detects freeze-dried powder respectively Color, HPLC content and dissolubility, the results are shown in Table 14.

The Accelerated stability test (37 DEG C) of flexible lipidosome freeze-dried powder of the table 13 containing active peptides

The stability long term test (25 DEG C) of flexible lipidosome freeze-dried powder of the table 14 containing active peptides

The result shows that the character and activity that first 3 month maintain sample of the freeze-dried powder in accelerated test, the 6th month activity Declined, Ying Jinliang avoids high temperature from storing.And freeze-dried powder maintains preferable character and activity in 25 DEG C of long term test, It can guarantee that the activity of storage in 2 years is not lost.

The preparation of the essence of the flexible lipidosome of the invention containing active peptides of embodiment 4

Flexible lipidosome of the present invention containing active peptides can be configured to essence use with solvent.The formula of solvent are as follows: Hyaluronic acid 0.3%, allantoin 0.1%, collagen 0.5%, EDTA 0.1%, multivitamin 0.15%.Above-mentioned formula It is aseptic subpackaged at the bottled solvent of 10~30ml.In use, the flexible lipidosome of 1 bottle of freeze-drying is dissolved with 1 bottle of solvent, essence is obtained Hua Su is applied directly on the face after morning and evening face cleaning.

The using effect of the essence of the flexible lipidosome of test example 4-1 acetyl-containing hexapeptide -8 is verified:

Experimental subjects: volunteer 108 people of the age bracket at 40-60 years old；

Experimental method: volunteer is randomly divided into two groups, the flexible rouge of one group of acetyl-containing hexapeptide -8 prepared for embodiment 4 The essence of plastid, another group is the solvent without flexible lipidosome.Daily morning and evening after face cleaning, two groups of essences are applied to respectively Face, is gently massaged into absorption, no longer smears other skin care item.The 2-6 weeks period on probation fills in using effect statistics after on probation Table, main indicator include the functions such as skin nursing, removal microgroove.

Experimental result: the essence throughout the country, such as Chengdu, Xichang, Tibet, Chongqing, Wuhan, Hainan, Shenzhen examination With 108 person-times, trial effect evaluation preferably (see Table 1 for details 5), while not occurring 1 allergic symptom, show the safety of the essence Property is good.

The essence of the flexible lipidosome of the acetyl-containing hexapeptide -8 of the present invention of table 15 uses result

Project	Number on probation	It uses time (week)	Effect is obvious (%)	Effect is unobvious (%)
					Skin nursing	108	2-6	82	18
Remove wrinkle	108	4-6	88	12

From the above results: the flexible lipidosome of acetyl-containing hexapeptide -8 of the present invention can be applied on cosmetics, Transdermal high-efficient, effect is good, the effect that the cosmetics of preparation have been all shown in nursing, crease-resistant face, before wide application Scape.

The using effect of the essence of the flexible lipidosome of test example 4-2 tetrapeptide containing palmityl -7 is verified:

Experimental subjects: volunteer 188 people of the age bracket at 40-60 years old；

Experimental method: volunteer is randomly divided into two groups, the flexible rouge of one group of tetrapeptide containing palmityl -7 prepared for embodiment 4 The essence of plastid, another group is the solvent without flexible lipidosome.Daily morning and evening after face cleaning, two groups of essences are applied to respectively Face, is gently massaged into absorption, no longer smears other skin care item.The 2-6 weeks period on probation fills in using effect statistics after on probation Table, main indicator include the functions such as skin nursing, removal microgroove.

Experimental result: the essence throughout the country, such as Chengdu, Xichang, Tibet, Chongqing, Wuhan, Hainan, Shenzhen examination With nearly 200 person-times, trial effect evaluation preferably (see Table 1 for details 6), while not occurring 1 allergic symptom, show the peace of the essence Full property is good.

The essence of the flexible lipidosome of the tetrapeptide containing palmityl -7 of the invention of table 16 uses result

From the above results: the flexible lipidosome of present invention tetrapeptide containing palmityl -7 can be applied on cosmetics, Transdermal high-efficient, effect is good, and the cosmetics of preparation are outstanding in free radical resisting, the performance of anti-oxidant aspect, and anti-acne ecchymose removing, sunburn are repaired Effect with having all shown in terms of antiallergic, has broad application prospects.

The using effect of the essence of flexible lipidosome of the test example 4-3 containing Matrixyl -4 is verified:

Experimental subjects: volunteer 90 people of the age bracket at 40-60 years old；

Experimental method: volunteer is randomly divided into two groups, one group of flexible rouge containing Matrixyl -4 prepared for embodiment 4 The essence of plastid, another group is the solvent without flexible lipidosome.Daily morning and evening after face cleaning, two groups of essences are applied to respectively Face, is gently massaged into absorption, no longer smears other skin care item.The 2-6 weeks period on probation fills in using effect statistics after on probation Table, main indicator include skin nursing, removal microgroove, the elasticity functions such as smooth.

Experimental result: the essence throughout the country, such as Chengdu, Xichang, Tibet, Chongqing, Wuhan, Hainan, Shenzhen examination With nearly 100 person-times, trial effect evaluation preferably (see Table 1 for details 7), while not occurring 1 allergic symptom, show the peace of the essence Full property is good.

The essence of the flexible lipidosome of the invention containing Matrixyl -4 of table 17 uses result

Project	Number on probation	It uses time (week)	Effect is obvious (%)	Effect is unobvious (%)
					Skin nursing	90	2-6	92	8
Remove wrinkle	90	4-6	84	16
					Elasticity is smooth	90	2-6	90	10

From the above results: flexible lipidosome of the present invention containing Matrixyl -4 can be applied on cosmetics, Transdermal high-efficient, effect is good, the effect that the cosmetics of preparation have been all shown at skin nursing, crease-resistant, the smooth aspect of elasticity, tool Have broad application prospects.

The using effect of the essence of flexible lipidosome of the test example 4-4 containing nonapeptide -1 is verified:

Experimental subjects: volunteer 80 people of the age bracket at 20-40 years old；

Experimental method: volunteer is randomly divided into two groups, one group of flexible lipidosome containing nonapeptide -1 for the preparation of embodiment 4 Essence, another group is the solvent without flexible lipidosome.Daily morning and evening after face cleaning, two groups of essences are applied to face respectively, It is gently massaged into absorption, no longer smears other skin care item.In the 4-8 weeks period on probation, using effect statistical form is filled in after on probation, mainly Index includes the functions such as skin nursing, whitening, nti-freckle.

Experimental result: the essence throughout the country, such as Chengdu, Xichang, Tibet, Chongqing, Wuhan, Hainan, Shenzhen examination With nearly 100 person-times, trial effect evaluation preferably (see Table 1 for details 8), while not occurring 1 allergic symptom, show the peace of the essence Full property is good.

The essence of the flexible lipidosome of the invention containing nonapeptide -1 of table 18 uses result

Project	Number on probation	It uses time (week)	Effect is obvious (%)	Effect is unobvious (%)
					Skin nursing	80	4-8	85	15
Whitening	80	4-8	88	12
					Nti-freckle	30	4-8	80	20

From the above results: flexible lipidosome of the present invention containing nonapeptide -1 can be applied on cosmetics, transdermal effect Rate is high, and effect is good, and the effect that the cosmetics of preparation have been all shown in terms of whitening, nti-freckle has broad application prospects.

Sequence table

<110>Shenzhen nobleness Ke Mei Biotechnology Co., Ltd

<120>comprising the flexible lipidosome cosmetics and preparation method thereof of active peptides

<130> A190061K

<141> 2019-01-30

<150> 201810150521.3

<151> 2018-02-13

<160> 2

<170> SIPOSequenceListing 1.0

<210> 1

<211> 12

<212> PRT

<213>artificial sequence (Artificial Sequence)

<400> 1

Val Gln Trp Arg Ile Arg Val Ala Val Ile Arg Lys

1 5 10

<210> 2

<211> 9

<212> PRT

<213>artificial sequence (Artificial Sequence)

<400> 2

Met Pro Phe Arg Trp Phe Lys Pro Val

1 5

Claims

1. including the flexible lipidosome cosmetics of active peptides, it is characterised in that: be by the peptide modified soft of hydrophobization modification Property liposome include active peptides made of.

2. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the hydrophobization The flexible lipidosome of modified polypeptide modification is made of the raw material of following main component: by weight, lecithin: NaTDC Or polysorbate80 or polysorbate80 derivative=7:2-4, contain the hydrophobization that weight percent is 1-10% and modifies Polypeptide also contains active peptides.

3. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: also contain antioxygen Agent.

4. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: described anti-oxidant Agent includes: at least one of vitamin C or derivatives thereof, vitamin E or derivatives thereof or ubiquinone.

5. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: described anti-oxidant The weight percent of agent is 0.1-1%.

6. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the activity Polypeptide is can be in the active peptides that cosmetic field uses.

7. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: described to change The active peptides that cosmetic field uses be with crease-resistant class, free radical resisting class, promote cell activity class, whitening class, detumescence class or Promote the active peptides of at least one of eyelashes growth class function.

8. the flexible lipidosome cosmetics according to claim 2 comprising active peptides, it is characterised in that: the activity is more Peptide specifically includes copper victory peptide, dipeptides -2, palmityl tripeptides, palmityl tripeptides -1, palmityl tripeptides -5, palmityl tetrapeptide -7, second Acyl group tetrapeptide -5, acetyl group tetrapeptide -9, Matrixyl -3, Matrixyl -4, Argireline, acetyl group octapeptide, meat At least one of myristoyl pentapeptide -17, myristoyl hexapeptide -4, nonapeptide -1, carnosine, N-BETA-Alanyl-L-histidine or glutathione.

9. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the activity is more The weight percent of peptide is 0.01~1%.

10. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, which is characterized in that described is soft Property liposome is prepared by the ingredient of following proportions: by weight, lecithin: NaTDC or polysorbate80 or Polysorbate80 derivative=7:3, the antioxidant for being 0.5% containing weight percent are also 2- containing weight percent 10% hydrophobization modified polypeptide.

11. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: described hydrophobic The sequence for changing polypeptide in modified polypeptide is to connect NH in C-terminal on the basis of SEQ ID NO:1₂, and be coupled in the nitrogen end of polypeptide Phytosterin compound or saturated straight chain fatty acid.

12. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the steroid Alcohol compound is cholesterol compound or Cholic acids compound.

13. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the steroid Alcohol compound is at least one of cholesterol, succinylated cholesterol, cholic acid or deoxycholic aicd.

14. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the saturation Straight chain fatty acid is at least one of C6~C20.

15. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the saturation Straight chain fatty acid is at least one of C8~C18.

16. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the length Chain fatty acid is at least one of stearic acid, palmitic acid, lauric acid or caprylic acid.

17. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: described hydrophobic Change modified polypeptide structure are as follows:

Wherein, institute The R stated is phytosterin compound or saturated straight chain fatty acid.

18. the flexible lipidosome cosmetics according to claim 17 comprising active peptides, it is characterised in that: the R For

19. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: described is anti- The class active peptides that wrinkle are at least one of palmityl tripeptides, Argireline or acetyl group octapeptide.

20. the flexible lipidosome cosmetics according to claim 19 comprising active peptides, it is characterised in that: described is anti- It is 0.01~3% that wrinkle class active peptides, which account for Via Liposomes weight percent,.

21. the flexible lipidosome cosmetics according to claim 19 comprising active peptides, it is characterised in that: described is anti- It is 0.05~2% that wrinkle class active peptides, which account for Via Liposomes weight percent,.

22. the flexible lipidosome cosmetics according to claim 19 comprising active peptides, it is characterised in that: described is anti- It is 0.1~1% that wrinkle class active peptides, which account for Via Liposomes weight percent,.

23. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: described is anti- Radical type active peptides are at least one of glutathione, palmityl tetrapeptide -7, carnosine or N-BETA-Alanyl-L-histidine.

24. the flexible lipidosome cosmetics according to claim 23 comprising active peptides, it is characterised in that: described is anti- It is 0.01~3% that radical type active peptides, which account for Via Liposomes weight percent,.

25. the flexible lipidosome cosmetics according to claim 23 comprising active peptides, it is characterised in that: described is anti- It is 0.05~2% that radical type active peptides, which account for Via Liposomes weight percent,.

26. the flexible lipidosome cosmetics according to claim 23 comprising active peptides, it is characterised in that: described is anti- It is 0.1~1% that radical type active peptides, which account for Via Liposomes weight percent,.

27. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: the rush It is Matrixyl -3, Matrixyl -4, copper victory peptide, palmityl tripeptides -1, palmityl three into cell activity class active peptides At least one of peptide -5, acetyl group tetrapeptide -9 or myristoyl hexapeptide -4.

28. the flexible lipidosome cosmetics according to claim 27 comprising active peptides, it is characterised in that: the rush Accounting for Via Liposomes weight percent into cell activity class active peptides is 0.01~3%.

29. the flexible lipidosome cosmetics according to claim 27 comprising active peptides, it is characterised in that: the rush Accounting for Via Liposomes weight percent into cell activity class active peptides is 0.05~2%.

30. the flexible lipidosome cosmetics according to claim 27 comprising active peptides, it is characterised in that: the rush Accounting for Via Liposomes weight percent into cell activity class active peptides is 0.1~1%.

31. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: the beauty White class active peptides are nonapeptide -1.

32. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: described disappears Swollen class active peptides are acetyl group tetrapeptide -5 or dipeptides -2.

33. the flexible lipidosome cosmetics according to claim 7 comprising active peptides, it is characterised in that: the rush It is myristoyl pentapeptide -17 that eyelashes, which grow class active peptides,.

34. according to the described in any item flexible lipidosome cosmetics comprising active peptides of claim 31-33, feature exists In: the whitening, detumescence promote eyelashes growth class active peptides to account for Via Liposomes weight percent to be 0.01~3%.

35. according to the described in any item flexible lipidosome cosmetics comprising active peptides of claim 31-33, feature exists In: the whitening, detumescence promote eyelashes growth class active peptides to account for Via Liposomes weight percent to be 0.05~2%.

36. according to the described in any item flexible lipidosome cosmetics comprising active peptides of claim 31-33, feature exists In: the whitening, detumescence promote eyelashes growth class active peptides to account for Via Liposomes weight percent to be 0.1~1%.

37. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the packet Flexible lipidosome cosmetics containing active peptides further include having emulsifier related with cosmetics are prepared, assistant for emulsifying agent, skin tune Manage agent, brightening agent, colorant, moisturizer, solubilizer, surfactant, preservative, aromatic, emollient, anti-acne agent, at At least one of film, thickener, pH adjusting agent, buffer, stabilizer or ultraviolet absorbing agent.

38. the flexible lipidosome cosmetics according to claim 1 comprising active peptides, it is characterised in that: the packet The dosage form of flexible lipidosome cosmetics containing active peptides is aqua, emulsion, paste, gelatin dose, ingot shape dosage form or vapour colloidal sol Type dosage form.

39. including the flexible lipidosome cosmetics of active peptides according to claim 38, it is characterised in that: the packet The dosage form of flexible lipidosome cosmetics containing active peptides is lotion, Essence, face cream, facial mask, freeze-dried powder or facial cleanser.

40. the method for preparing the described in any item flexible lipidosome cosmetics comprising active peptides of claim 1-39, special Sign is: the following steps are included:

A, lecithin, NaTDC or polysorbate80 or derivatives thereof are weighed, vitamin E is placed in reaction vessel, add Enter solvent dissolution, the solvent is the mixture or all ethyl alcohol that chloroform and ethyl alcohol press 1: 1-3 composition；

B, rotary evaporation in vacuo obtains liposome membrane, dry；

C, DP7-C or PAL-DP7 and distilled water are added in liposome membrane, carries out ultrasonic aquation and obtains blank liposome solutions；

D, active peptides are added in blank liposomes liquid solution, it is slight to shake, 30-60 minutes are incubated at room temperature to get activity is included The flexible lipidosome of polypeptide.

41. the method for flexible lipidosome cosmetics of the preparation comprising active peptides according to claim 40, feature exist In: further include e: liposome being pressed through 0.1-0.45 μm of polycarbonate membrane 4~8 times, blank liposome solutions are obtained.

42. the method for flexible lipidosome cosmetics of the preparation comprising active peptides according to claim 40, feature exist In: further include step f: by dosage form requirement, flexible lipidosome is added again prepare the related emulsifier of cosmetics, assistant for emulsifying agent, Skin conditioning agent, brightening agent, colorant, moisturizer, solubilizer, surfactant, preservative, aromatic, emollient, anti-acne Cosmetics agent is made at least one of agent, film forming agent, thickener, pH adjusting agent, buffer, stabilizer or ultraviolet absorbing agent Type.