KR20120037639A - Capsulation method and cosmetic containing this capsulation for maximizing efficacy of sodium polygamma glutamate - Google Patents

Abstract

PURPOSE: A cosmetic product containing sodium poly gamma glutamate is provided to absorb a stabilized capsule composition into skin epidermis and to strengthen skin barrier. CONSTITUTION: A capsulated material contains 0.001-20 wt% of sodium poly gamma glutamate as a main ingredient and 0.001-20 wt% of hydrogenated lecithin and derivative thereof, 0.001-10 wt% of sterol and derivative thereof as a capsule membrane forming agent, and 0.001-3 wt% of polyquaternium-11 and derivative thereof as an ion charge forming material.

Description

Capsulation method and Cosmetic containing this capsulation for maximizing efficacy of Sodium polygamma glutamate}

The present invention relates to a method for encapsulating a polymer sodium polygamma glutamate, which is a potent ingredient for cosmetics, and to encapsulating the potency of the cosmetics. More specifically, the present invention relates to a composition comprising sodium polygamma glutamate incorporated as a main component and hydrogenated lecithin and its derivatives, phytosterols and derivatives thereof, which is a minor component used as a wall reinforcing material used for encapsulation. In addition, a method for imparting an ion charge to the surface of the membrane in order to further stabilize the capsule membrane, a compound comprising polyquaternium-11 and derivatives thereof and a composition prepared by passing one or several times through a high pressure homomixer for such a composition It is about the process to make. The encapsulated and stabilized composition of the present invention is delivered and absorbed not only to the skin epidermal layer but also to the inner epidermal layer, thereby improving skin aging or aging through skin moisturizing and supplying function, increase in the number of bonds, skin barrier strengthening, and evaporation suppression of skin moisture. Has the effect of delaying. Therefore, it has the use of general external skin preparations, external skin preparations of cosmetic products, or cosmetic compositions.

[Prior Art Literature]

Poly-γ-glutamate (poly-γ-glutamate) is a typical fermented food is a sticky mucus is produced when fermented soybeans using Bacillus subtilis from Korea's Cheonggukjang, Japan's Natdo. Fermentation of soybeans has resulted in the production of high molecular weight materials such as polygamma glutamate and polyfurctan, which have been reported to have moisturizing and cell activation functions. When used in conjunction with Taxol is known to increase the effect of the drug. In particular, it is reported that polyfurutic acid is a dietary fiber for diet, and when it is ingested in the human body, it can suppress appetite because it gives a feeling of satiety (Journal of Applied Microbiology, 92, 5, p958-963). . It only relates to the manufacturing method of the polymer polymer, and does not mention the content of specialized application as a cosmetic.

[Patent Documents]

Folate-contained polygamma glutamate is produced by fermenting a culture containing folic acid and soybean extract using a microorganism with a soybean fermentation polymer material and a composition patent (Korean Patent No. 10-09261700000) containing the same. Patented soybean fermented polymer material and a composition comprising the same. It has been reported that it has antihistamine, anti-allergic, calcium absorption, bone growth, collagen biosynthesis and wrinkle improvement.

However, when looking at the entire contents, including the embodiment of the composition patent, it is limited to the preparation of soybean fermented polymer material in which folic acid is incorporated, and as an application method for the skin external preparation and cosmetic composition by a simple formulation in the cosmetic of this material It is limited to patents. In the present invention, there is no mention of a patent for a method and an application for encapsulating a polymer having a very high molecular weight.

Soybean fermented extract patent (Korean Registered Patent No. 10-0451631) for cosmetic raw materials, Korean Patent Publication No. 2001-0083876, etc. have been reported as a patent for external skin preparations. They have developed a technique for producing polygamma glutamate having a molecular weight of 2,000 kDa or more by an enzymatic method (Application and Environ. Microbiol. 2004; 70 (7), 4249-4255; Korean Patent No. 10-0399091). However, since polygamma glutamate has a very strong ester bond, it is not easy to dissolve in water, and even if a small amount is dissolved, it can be seen through the present invention that it is not easy to obtain a substantial effect in the cosmetic field.

In the present invention, in order to increase the solubility of sodium polygamma glutamate, which is a commonly disclosed patent composition, the sodium salt-substituted sodium poly gamma glutamate as a main component is combined with the components used as the inside and phospholipid bilayer of the vesicle Forming a more stable vesicle can be said to be the technical appeal point of the present invention.

In general, since sodium polygamma glutamate is a polymer, there are no published techniques for encapsulating inside the endoplasmic reticulum. Because it is a high molecular material. The current application technology is known to have a moisturizing effect through a simple formulation in cosmetics, but there are many problems to prove a very good effect. The reason is that the polymer can not penetrate into the skin and can only expect the effect of simply being applied to the epidermis. Sodium hyaluronate, which is a skin constituent with a similar function, has a similar function.

Therefore, in the present invention, the encapsulation was attempted for the purpose of maximizing the efficacy of sodium polygamma glutamate, and the selection of the constituents of the selected capsule membrane to make the encapsulation is most important. In addition, it is also an important task to be solved in the present invention to have a cationic charge so that the sodium poly gamma glutamate can be attached to the capsule well. In addition, it is also one of important processes in the present invention to cut the polymer of sodium polygamma glutamate into small size. This process is intended to allow packing well inside the capsule or around the membrane forming the capsule. For more stable encapsulation, setting the optimum conditions of the machine (high pressure homomixer) used in the process and performing the performance evaluation and verification of the stable encapsulated formulation also increase the value of the present invention. I think it can play an important role.

As a solution to the present invention, the following experiment was performed.

First, sodium polygamma glutamate, a polymer used as a main component, is packed into the phospholipid vesicle and the phospholipid membrane. It can be said that the key composition of the present invention is to select a special auxiliary component so that the main component can be self-vesicle. Examples of the components used for the purpose of encapsulating sodium polygamma glutamate in the present invention include hydrogenetidritchine and phytosterol. Second, since sodium polygamma glutamate has an anion charge, it is also a very important technical point to use polyquaternium-11, which is selected to display a cationic charge on the surface of the vesicle, so that the capsule is well formed. Third, sodium polygamma glutamate and preservatives are blended and passed through a high pressure homomixer (microfluidizer) to produce a small sized vesicle. Passing conditions are, but are not limited to, passing under 50 Psi-100,000 Psi pressure conditions. Fourth, to verify performance differentiation with that of sodium polygammaglutamate is not encapsulated. Especially water holding capacity evaluation, skin moisture loss amount. In clinical trials, the topic of skin moisturizing effect and skin epidermal cell observation is an issue to be solved.

As an effect of the present invention, the technical part of stably encapsulating the amino acid-based polysodium polygamma glutamate made of a polymer has a great technical significance. In particular, when encapsulating sodium polygamma glutamate, the amount of water holding is increased by about 2.5 times or more than that of the non-encapsulation, and the skin moisturizing effect is significantly higher than that of the simple compounding. In addition, as a result of clinical trials for 4 to 6 weeks, the water loss of the skin decreases as the absorbency increases to the skin and the cell state of the skin is improved. In addition, it can be seen as an effect to be absorbed to the inside of the epidermal layer to facilitate the skin action, it is expected to be widely applied as a skin external preparation in the cosmetic field.

1 is a diagram schematically illustrating the encapsulation by packing sodium polygamma glutamate in the endoplasmic reticulum or between membranes.
2 is a chemical formula briefly showing the molecular structure of sodium polygamma glutamate.
Figure 3 is a graph showing the viscosity change when passing through a high-pressure homo mixer to make a vesicle.
4 is a graph showing the moisture holding capacity.
5 is a graph showing skin absorption.
Figure 6 is a graph showing the skin moisturizing effect clinical trial results.
7 is a graph showing the results of verifying the skin moisture loss amount.
Figure 8 is a photograph of the skin keratinocyte tissue. Skin keratin state before thin use, I photographed skin comparison state of skin keratin after use for six months.
9 is an electron micrograph of encapsulated sodium polygammaglutamate.

The present invention relates to a method for preparing an encapsulation and a composition containing the same, which is developed to stably encapsulate the polymer sodium polygamma glutamate, which is a main component, to exert its efficacy. Sodium polygamma glutamate of the present invention is incorporated as a main component, hydroquanity dressin and its derivatives, phytosterol, used as a wall reinforcing material used for encapsulation, phytosterol, in order to impart ionic charge to the capsule membrane polyquaternium-11 It is also an important technique of the present invention to optimize the process for preparing capsules by passing them through a high pressure homomixer, which is a machine used as a method for stabilizing capsules using the compounds and derivatives thereof and the components thereof. This section will be described in detail.

A. Definitions of terms used in the present invention

In the present invention, in order to avoid confusion in expression of terms, it will be clearly defined as follows.

1. Sodium polygamma glutamate: Used as a main ingredient in the present invention as an amino acid moisturizing ingredient.

2. Hydrogenated lecithin and its derivatives (Hydrogenated lecithin derivatives): Hydrogenated lecithin and its modified components including the same.

3. Phytosterols: Refers to phytosterols, soy sterols, generically with components having this skeleton.

4. Cationic materilas: Cationic charged polymers, especially polyquaternium-11.

5. Sodium hyaluronate: A component used in the comparison group that refers to a high molecular material of a moisturizing component.

6. Capsule: Collectively terminology used in the present invention.

7. Non-capsule: Refers to a composition of the present invention or generally unencapsulated.

8. in-vitro: A test term that refers to an in vitro test, not a clinical test, indirectly evaluating efficacy and performance.

9. in-vivo: A test term that evaluates performance through actual clinical trials.

B. Technical Background for Encapsulation

In general, sodium hyaluronate, which is a representative component of the skin, is known to have various effects on the skin, but is only applied to the skin surface and does not have a great effect on the tissue's physiological activity. It is known. It is used as a simple sales talk in some cosmetics sectors, and it is true that it is overpacked. Polygamma glutamate used in the present invention has also been thought to be generally not encapsulated because of its high molecular weight. In addition, since sodium polygamma glutamate selected in the present invention is also a polymer, it is impossible to absorb skin, and since its efficacy is not so excellent, it is a fact that it is not widely used for cosmetics at present.

The reason for introducing the encapsulation technique in the present invention is to focus on encapsulating sodium polygamma glutamate in the endoplasmic reticulum to be more absorbable to the skin. It is a means to overcome the shortcomings of this ingredient, and by contributing to the substantial efficacy of the skin, the main purpose is to enable the practical use in all related fields to make cosmetics for the purpose of developing a better cosmetic raw material.

C. Selection of Encapsulation Compositions

In order to encapsulate sodium polygamma glutamate in the present invention, since it is not possible alone, it is important to select an auxiliary component to enable the capsule. As a secondary component of the capsule formation used in the present invention, hardogenetirescichin was selected so that a vesicle could be automatically formed. In addition to this substance, unsaturated lecithin, lysocithin, and the like may be included.Hardogenetidricitin, etc., refers to a main component of which contains less than 10% to 95% or more of phosphetidylcholine. It can be noted that it may be included in the material such as petidyl inositol to help stabilize the vesicle. In addition, phytosterol was used as an auxiliary component for forming vesicles, but is not necessarily limited thereto. Cholesterol and its derivatives are also included in this technique. In the present invention, phytosterol was recently selected as a preferred plant-derived material, but is not necessarily limited thereto. This material consists of a mixture of trace amounts of camphorsterol and stigmasterol, and it is noted that they help stabilize the vesicle. In the present invention, 0.001-10% by weight of phytosterol was used in a wide range. Specifically, 0.01-5% by weight of cholesterol and phytosterol were used as a wall reinforcing substance. Although vesicles can be produced even when hydrogenated alone without using this material, it can be seen from this experiment that vesicles can be made more stable than 0.01-5% by weight of cholesterol and phytosterol. there was.

Sodium polygamma glutamate, the main component inside the vesicles of the vesicle, has an anion charge of the capsule membrane with anion charge to make the capsule feasible.Sodium polygamma glutamate also has anion charge so that the capsule is unstable due to the repulsive force. It is possible that the capsule is broken or formed over time. In order to solve this problem, it is a key point of the present invention to change the circumference of the membrane of the endoplasmic reticulum into a cationic charge. Therefore, a more stable capsule could be made by mixing 0.001-3% by weight of polyquaternium-11. It is not necessarily limited to polyquaternium-11 as a method for cationic charge. These include cellulose-based polymers and quaternary polymers of quaternary ammonium salts, and include single or two or more sterol derivatives such as phospholipid hydrogenetidrecithin or derivatives thereof, cholesterol or phytosterol, with sodium polygamma glutamate as a main component. This technique includes all techniques applied through the process of mixing and forming the vesicles.

D. Micronized process to ensure vesicle packing

In order to refine the sodium polygamma glutamate, which is the main polymer of the present invention, to be packed inside the vesicles or between the membranes, a technical process of passing through a high pressure homomixer is important. Sodium polygamma glutamate is first mixed with water and first homogeneously dispersed at 1,000-10,000 rpm with a homodisper. Mix well with hydrogenetirescitin, phytosterol or cholesterol and derivatives thereof listed above, and polyquaternium-11 for cationic charge, and then pass through a high pressure homomixer. At this time, it may not pass well because the main component is a polymer. At this time, it should be passed with caution, and it should be noted that the vesicle may become small or large depending on the number of passes. The passing pressure of the high pressure homomixer may vary depending on the characteristics of the commercialized machine and the manufacturer, but this includes a process characterized in that the passage is adjusted to a passing pressure of about 50 Psi-100,000 Psi. Specifically, passing at pressures of up to 100 Psi and up to 30,000 Psi is suitable for efficient encapsulation. However, not only this but also the encapsulation using a similar process is also included.

The size and stability of the vesicle may vary depending on the number of times passed through the high pressure homomixer to make the vesicles more stable. Therefore, in the present invention, the number of passes is most realistic in the range of 1 to 10 times. In consideration of economic efficiency and efficiency, it was found that the best vesicle is formed when passing through the encapsulant in detail from 3 to 6 times. It is not limited to the one disclosed in the contents of the present invention with respect to the number of passes, and the method of using the method similarly also corresponds to the present invention. Claims of techniques used in the present invention may be included in the following criteria.

[1] 0.001-20% by weight of sodium polygamma glutamate as a main component, while 0.001-20% by weight of hydrogenetidrecithin and its derivatives, 0.001-10% by weight of sterol and its derivatives are used as capsule film forming agents. An encapsulated material containing 0.001-3% by weight of polyquaternium-11 and derivatives thereof as an ion-charge forming material in the wall of its vesicle.

[2] The process according to claim 1, wherein a process using a high pressure homomixer (microfluidizer) and the like is used for capsule and stabilization purposes, and the pressure is used in the high pressure homomixer in the range of 50 Psi to 100,000 Psi. Process.

[3] The method according to claim 1, which is more specifically 0.01 to 20% by weight sodium polygamma glutamate as a main component, 0.1 to 10% by weight hydrogenetirescithin, 0.01 to 5% by weight cholesterol and phytosterol, A composition containing 0.001 to 1% by weight of polyquaternium-11 as an ion-charge forming agent used as a wall-reinforcement material, and a process for stabilizing capsules through a high pressure homomixer (microfluidizer) for the purpose of encapsulation. In this case, the detailed through pressure at this time is a method of manufacturing by passing through the process to a small 100Psi, large 30,000Psi.

[4] The method according to any one of claims 1 to 3, wherein the polymer sodium polygamma glutamate has a molecular weight of 10,000 Da to 2,000,000 Da or encapsulates or encapsulates in a vesicle, To maximize the collection capacity within the capsule, or to facilitate absorption and penetration into the skin.

[5] The method according to any one of claims 1 to 3, which is used as an external skin preparation to strengthen the skin barrier, and provides excellent moisturizing effect, skin moisture supply effect, water loss reduction effect, water holding capacity increase, and skin condition improvement. Mixed cosmetic composition characterized in that it has a technique of strengthening the skin barrier by the action of such.

[6] The composition according to any one of claims 1 to 3, wherein the composition includes a composition incorporated as an external ingredient for improving skin wrinkles to restore healthy skin and delaying skin aging. Cosmetics such as skin lotion, latex lotion, beauty essence, nourishing cream, sunscreen cream, massage cream, cleansing cream, BB cream, foundation, wrinkle improvement and whitening cream, skin elasticity and moisturizing cream.

The capsule stabilized composition of the present invention may be delivered not only to the skin epidermal layer but also to the inner epidermal layer, and may have a skin moisturizing effect, an increase in the number of bonds, and a skin barrier strengthening function. As a potent effect of this technology, the water holding capacity is significantly increased, and it has the effect of improving the skin's aging or delaying the aging through the action of inhibiting the evaporation of skin moisture. Therefore, it has the use of general external skin preparations, external skin preparations of cosmetic products, or cosmetic compositions.

Optimization of Encapsulation Composition of Sodium Polygammaglutamate

A composition for encapsulation of was prepared, based on sodium polygammaglutamate. A composition was prepared as described in Example 1, incorporating hydrogeneticrescitin, phytosterol, polyquaternium-11 as an auxiliary ingredient for stable encapsulation.

Table 1. Encapsulation Composition of Sodium Polygamma Glutamate (wt%)

As shown in the composition shown in Composition 1 to Composition 5 in Example 1, the composition of sodium polygamma glutamate corresponding to the main component of the present invention was listed from 0.1% by weight to 10% by weight, and as a secondary component 0.1 wt% to 5 wt% hydrogenetirescichin, 0.3 wt% unsaturated lecithin, 0.1 wt% to 5 wt% phytosterol, 0.1 wt% to 5 wt% cholesterol, 0.001 wt% to 1 wt% polyquater The composition for mixing nium-11 was described. Comparative 1 shows the composition by the general simple blending. The composition for the present invention is not simply limited to the conditions listed in the Examples, but the case of mixing the components shown here alone or two or more is applicable to the present invention, more reliably all encapsulated sodium polygamma glutamate Note that the process and composition is equivalent to this.

Process Optimization Conditions of High Pressure Homo Mixer for Stable Encapsulation

Process optimization conditions using a high pressure homomixer in order to make the polymer sodium polygamma glutamate into a smaller and more stable capsule with the composition included in Example 1 are shown in Example 2. The pretreatment method for passing the high pressure homo mixer on the basis of the composition 2 of Example 1 will be described. First, sodium polygamma glutamate is mixed with water, stirred for 30 minutes at 1,000-10,000 rpm with a disper mixer, and then the capsule auxiliary component is dissolved in warm water at 65-80 ° C. At this time, it should be stirred while being careful not to generate bubbles. After cooling this to 45 degreeC, it mixes with the sodium poly gamma glutamate aqueous solution. Pass this through a high pressure homomixer. The temperature is also possible at room temperature, and the conditions at the time of passage are shown in detail in FIG. 3. First of all, the process can be as small as 100 Psi and up to 30,000 Psi. More specifically, after fixing at 10,000 Psi, the number of passes is possible from 1 to 10 times, but considering the efficiency of the work, the number of passes was the best in the range of 3 to 6 times (Fig. 3). . Repeated from 1 to 2 times, the viscosity of the capsule could be seen to decrease. This can be said that the polymer is converted into a capsule of very small size. On the other hand, in the case of using a general emulsifier other than the vesicle as shown in Comparative Group 1, the encapsulation is impossible, and even though it is possible to pass through the high pressure homomixer, the viscosity decreases, but it was found that it is unstable.

Performance Evaluation of Encapsulated Sodium Polygamma Glutamate

In order to evaluate the performance of the encapsulated sodium polygamma glutamate, the composition of Table 2 of the encapsulated prescription 6 and the composition 7 of the non-encapsulated prescription were made into the same concentration of sodium hyaluronate and the moisture holding capacity was measured. The performance was measured and shown in FIG. The measuring method of water holding capacity is fixed to an incubator that can control temperature and humidity at a temperature of 50 ° C. and a humidity of 80 RH%. Then, each sample is added and the amount of water is measured with a moisture meter skincon-200 as shown in FIG. 4. Indicated. As shown in Fig. 4, the sodium hyaluronate of Comparative 2 had a water holding capacity of 6.7% after 4 hours, and a holding amount of 19.8% in the composition 7 which was a non-capsule. However, in the encapsulated invention (composition 6) in the present invention, the water holding capacity was found to contain 48.9%.

Table 2. Evaluation composition of water holding capacity (% by weight)

This was about 7 times more effective than Comparative 2, and was found to have a water retention of about 2.5 times higher than that of non-capsules. This proves that the technique of encapsulated sodium polygamma glutamate is superior to the general formulated ingredients.

Performance Evaluation on Skin Absorption

Performance evaluation of the skin absorption was applied to the same sample as shown in Table 2 to evaluate the skin absorption by the sensory evaluation of the subject. The subjects' choices were assessed on a 10-point scale with 10 male and female college students majoring in cosmetics at the age of 20. The contents of the sensory evaluation were five items: softness, absorbency, moisturizing power, and stickiness (the lower the value, the better the result), and the effect.The evaluation was applied twice a day and alternately on the back of both hands for 5 days. Self-satisfaction was shown (FIG. 5). As shown in the results, the soft texture was obtained at the same level as the capsule (invented product) and the non-capsule, but the results were significantly different in the absorption, moisturizing, and effect items. In the case of the sodium hyaluronate polymer of Comparative 2, the stickiness tends to be strong, and all the items did not show very good results. As shown here, it was found that the clinical trial and sensory evaluation did not show a very good effect in the actual clinical trial, even if the in-vitro test showed an excellent effect. It was found that even if the beneficial ingredients for the skin, the difference in effect is involved in the skin absorption and synergism of the other ingredients.

Evaluation of skin moisturizing effect In-vivo test

In the performance evaluation of the clinical trial of the skin moisturizing effect, the same sample as shown in Table 2 was applied to evaluate the skin moisturizing condition of the subject. The subjects were selected from 30 male and female college students with a 20-year-old cosmetology major. After each three samples were corrected twice a day for 4 weeks, the amount of skin moisture before application and the amount of skin moisture after 4 weeks were measured. As the measurement equipment, the skin moisturizer (Skicon-200; Skicon-200), which is most frequently used in dermatology and cosmetics laboratories, was used in the laboratory that can be incubated before the measurement, and the temperature condition was 25 ° C / 40RH%. The measurement was performed after setting. As shown in FIG. 6, the skin moisturizing effect (in-vivo) before application was measured and averaged for each of 10 patients. As a result, 42.5% to 43.7% of the three groups showed a similar moisturizing effect. The moisturizing effect on the three compositions after care was as follows. The average moisturizing power after 4 weeks in the non-capsule was 51.6%, which was about 8% higher than before application. This result was not statistically significant as a result of statistical treatment with P-test. Also in the case of Comparative 2 sample, the moisturizing effect before application was found to have 46.2% of moisturizing power after 4 weeks. This was found to have an effect of about 4% than before application. However, the skin moisturizing power after 4 weeks of the capsule preparation made by the technique of the present invention was 42.5% to 71.6% before application, showing a significant moisturizing synergistic effect of more than about 29% (Fig. 6). This is considered to be a synergistic effect as the main component of sodium polygamma glutamate encapsulated using phospholipid is absorbed into the skin, and it can be considered that the skin condition and moisturizing effect are greatly improved when this component is used continuously. In addition, it can be predicted that a high effect can be obtained by synergy of various components of the encapsulating material.

Performance evaluation through skin in-vivo evaluation

This test (in-vivo) can determine whether the skin barrier strengthening effect through the trans-epidermal water loss (TEWL). This evaluation was evaluated by applying the same sample as the composition shown in Table 2. The subjects were selected from 30 male and female college students who are majoring in cosmetics at the age of 20. After each three samples were corrected twice a day for 6 weeks, the amount of skin moisture loss before application and TEWL after 6 weeks were measured to evaluate whether the skin condition was changed. The TEWL meter, which is most frequently used in dermatology and cosmetics laboratories, was used as a measurement equipment.The measurement was carried out in an incubation room where temperature and humidity were controlled before measurement.The temperature condition was set to 25 ° C / 40RH%. The measurement was performed. As shown in FIG. 7, the TEWL effect before application was measured and averaged for each 10 persons. As a result, the skin barrier was strengthened by measuring the amount of reduced skin moisture loss by 100% before application, thereby improving skin health. The performance for was evaluated. After 6 weeks of non-capsules, TEWL was 87.9%, which was about 12% lower than before application. This result showed that there was no significant difference as a result of statistical treatment with P-test (Fig. 7). . On the other hand, even in Comparative 2 sample, the TEWL after 6 weeks was reduced by 96.6% to 4.4%, so that it did not decrease much before application, indicating that the skin barrier did not change significantly. However, the TEWL after 6 weeks of the capsule preparation made by the technique of the present invention was 68.9%, and the water loss amount of about 31% or more was reduced compared to before application, indicating that the skin barrier was significantly strengthened (FIG. 7). . This is considered to be a synergistic effect as the main component of the encapsulated sodium polygamma glutamate is absorbed into the skin, and it is a result that the effect is increased by continuous use for 6 weeks.

Skin Lotion Cosmetic Composition

The cosmetic of skin lotion using encapsulated sodium polygamma glutamate will be described by way of example. As shown in Table 3, glycerin, 1,3-butylene glycol, dipropylene glycol, Fiji-400, EDTA-2Na, betaine, and carbohydrate, including composition 2 of Example 1 as ingredients for making skin lotion This includes compositions containing bomber, hydroxyethylcellulose, potassium hydroxide, citric acid, sodium citrate, purified water, fiji-60 hydrogenated castor oil, ethanol, flavoring, tocopheryl acetate, phenyltrimethicone, and the like. . As an example for the emulsion in the present invention it is not limited to the composition shown in this example.

Table 3. Skin lotion cosmetics (% by weight)

Latex Lotion Cosmetic Composition

The cosmetic of an emulsion lotion using encapsulated sodium polygamma glutamate will be described by way of example. As shown in Table 4, the components necessary to make the latex lotion cosmetics including the components shown in Compositions 13, 14 and 15, including the composition 2 of Example 1 as the components for making the emulsion, are used herein. Yes. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. Note that the present invention is not limited to the composition shown in this example as an example of the emulsion lotion.

Table 4. Latex lotion (% by weight)

Cosmetic liquid essence cosmetic composition

The cosmetic of essence using encapsulated sodium polygamma glutamate will be described by way of example. As shown in Table 5, this is the case of using the components necessary to make cosmetic essence cosmetics, including the components shown in Compositions 16, 17 and 18, including Composition 2 of Example 1 as components for making cosmetic essences. do. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. As an example for the cosmetic essence cosmetic in the present invention it is not limited to the composition shown in this example.

Table 5 . Cosmetic essence (% by weight)

Nourishing cream cosmetic composition

An nourishing cream cosmetic using encapsulated sodium polygamma glutamate will be described by way of example. As shown in Table 6, the ingredients necessary to make the nourishing cream cosmetics including the ingredients shown in Compositions 19, 20 and 21, including the composition 2 of Example 1 as ingredients for making the nourishing cream, are used herein. Yes. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. In the present invention, as an example for the nourishing cream cosmetics it is not limited to the composition shown in this example.

Table 6. Nourishing cream cosmetics (% by weight)

Sunscreen cosmetic composition for sun protection

The cosmetic of the sunscreen cream using the encapsulated sodium polygamma glutamate will be described as an example. As shown in Table 7, this is the case of using the ingredients necessary to make the sunscreen cosmetic for the sunscreen including the composition shown in composition 22, including the composition 2 of Example 1 as the ingredient for the sunscreen for the sunscreen. . The sunscreen value has the effect of SPF50 and PA +++. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. As an example for the sunscreen cosmetics in the present invention it is not limited to the composition shown in this embodiment.

Table 7. Cosmetic of Sunprotect Cream SPF50 / PA +++ (wt%)

Massage cream cosmetic composition

Massage cream cosmetics using the encapsulated sodium polygamma glutamate will be described by way of examples. As shown in Table 8, this is the case of using the components necessary to make the massage cream cosmetics, including the components shown in the composition 23, including the composition 2 of Example 1 as a component for making the massage cream. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. Note that the present invention is not limited to the composition shown in the present embodiment as an example for the massage cream cosmetic.

Table 8. Massage cream cosmetics (wt%)

Cleansing cream cosmetic composition

An example of a cleansing cream cosmetic using encapsulated sodium polygamma glutamate will be described. As shown in Table 9, this is the case of using the components necessary to make the cleansing cream cosmetics, including the composition 2 of Example 1 as the ingredients for making the cleansing cream, including the components shown in the composition 24. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. As an example for the cleansing cream cosmetics in the present invention it is not limited to the composition shown in this example.

Table 9. Cleansing cream cosmetics (% by weight)

BB cream cosmetic composition

BB cream cosmetics using encapsulated sodium polygamma glutamate will be described by way of examples. As shown in Table 10, this is the case of using the components necessary to make the BB cream cosmetics, including the components shown in the composition 25, including the composition 2 of Example 1 as the ingredients for the BB cream. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. As an example for the BB cream cosmetics in the present invention it is not limited to the composition shown in this example.

Table 10. BB cream cosmetic composition (% by weight)

Foundation Cream Cosmetic Composition

The cosmetic of the foundation cream using the encapsulated sodium polygamma glutamate will be described as an example. As shown in Table 11, this is the case of using the components necessary to make the foundation cream cosmetics, including the composition 2 of Example 1 as the component for making the foundation cream, and the composition shown in the composition 26. In the manufacturing method, kana and me were each weighed and dissolved by heating at 60-80 ° C., and then mixed and stirred homogeneously at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. Note that the present invention is not limited to the composition shown in this example as an example for the foundation cosmetics.

Table 11. Foundation cream (% by weight)

Wrinkle improvement and whitening cream cosmetic composition

Wrinkle improvement and whitening cream cosmetics using encapsulated sodium polygamma glutamate will be described with examples. As shown in Table 12, the ingredients necessary to make the wrinkle improvement and whitening cream cosmetics, including the components shown in Compositions 27 and 28, including the composition 2 of Example 1 as the ingredients for making the wrinkle improvement and whitening cream cosmetics This is the case. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. It should be noted that the present invention is not limited to the composition shown in this example as an example for the wrinkle improvement and whitening cream cosmetics.

Table 12 . Wrinkle improvement and whitening cream cosmetics (wt%)

Skin elasticity and moisturizing cream cosmetic composition

Skin elasticity and moisturizing cream cosmetics using encapsulated sodium polygamma glutamate will be described by way of examples. As shown in Table 13, the ingredients necessary to make the skin elasticity and moisturizing cream cosmetics including the components shown in Compositions 29 and 30, including the composition 2 of Example 1 as the ingredients for making the skin elasticity and moisturizing cream cosmetics, are used. This is the case. In the manufacturing method, kana and me were respectively weighed and dissolved by heating at 60-80 ° C., followed by mixing and homogeneously stirring at 3000-5000 rpm using a homomixer. After stirring homogeneously, the solution is neutralized and then cooled to 30 ° C., followed by vacuum defoaming to complete the preparation. As an example for the emulsion in the present invention it is not limited to the composition shown in this example.

Table 12. Skin elasticity and moisturizing cream cosmetics (wt%)

Skin clinical evaluation Improve skin stratum corneum

In order to measure the efficacy of the encapsulated sodium polygamma glutamate in the present invention, a clinical trial (in-vivo) was conducted on 30 men and women aged 20-40 years. The evaluation method used the composition 6, the composition 7, and the composition 2 of Table 2 of Example 3 as it was. Each of these samples was divided into three groups to form a square of 2 cm in width and length at the lower forearm, and each sample was applied twice a day for six weeks each morning and evening. The stratum corneum layer shown in Fig. 8 is peeled and dyed and photographed under a microscope. The photograph of FIG. 8 is a skin condition before applying the encapsulated sodium polygamma glutamate (FIG. 8A), and the photograph of I of FIG. 8 taken with a microscope to peel off keratin after 6 weeks of use of the encapsulated sodium polygamma glutamate. to be. Although the results of the composition 7 (non-capsules) and the comparison 2 are not described in the present invention, it was found that the skin condition of the encapsulated sodium polygamma glutamate is excellent in efficacy. The keratin condition before use showed irregular skin condition, that is, a rough skin condition due to the accumulation of multiple layers of texture, but the keratin condition after six weeks clearly showed that the keratin of the hexagon was clearly reproduced and the skin condition was improved. Compared with the non-capsules, which are not shown in the photograph, the prescriptions of Comparative 2 showed not improved skin keratin status. Therefore, it was confirmed that the cosmetic composition made using the composition of the present invention showed a very excellent effect.

Shape Observation of Vesicles Containing Encapsulated Sodium Polygammaglutamate

In order to observe the shape of the sodium polygamma glutamate encapsulated in the present invention, the shape and structure were observed using an electron microscope. The composition was observed with an electron microscope using the composition 6 of Example 3 and Table 2 as it was. As shown in Fig. 9, it was found that a vesicle containing the main component of the encapsulated sodium polygamma glutamate was formed. Its shape was confirmed that the double layer around the membrane of the capsule was formed, it was confirmed that the sodium polygamma glutamate trapped therein. This formed the same structure even after 6 months and it was confirmed that the thermodynamic is not so much a problem.

Therefore, this technical value that makes the encapsulation of the polymer that is impossible to encapsulate used for the first time in the present invention is considered to be applicable to a large number of fields, it is not necessarily the composition used herein, but the embodiment is not all want.

Claims (6)

It is a vesicle containing 0.001-20% by weight of sodium polygamma glutamate as a main component, and 0.001-20% by weight of hydrogenetidrecithin and its derivatives, 0.001-10% by weight of sterol and its derivatives as capsule forming agents. An encapsulated material containing 0.001-3% by weight of polyquaternium-11 and derivatives thereof as an ion-charge forming material in the wall of the film. The process according to claim 1, wherein a process using a high pressure homomixer (microfluidizer) and the like method is used for capsule and stabilization purposes, and the high pressure homomixer uses a passing pressure in the range of 50 Psi to 100,000 Psi. According to claim 1, More specifically, 0.01 to 20% by weight of sodium polygamma glutamate as a main component, 0.1 to 10% by weight of hydrogenetirescithin, 0.01 to 5% by weight of cholesterol and phytosterol, wall reinforcing material In the case of using a process for stabilizing the capsule by passing through a high-pressure homomixer (microfluidizer) for the purpose of encapsulating it as a composition containing 0.001 to 1% by weight of polyquaternium-11 as an ion charge former The specific through pressure of the process is manufactured by passing the process up to 100Psi, and 30,000Psi. The method according to any one of claims 1 to 3, wherein the polymer sodium polygamma glutamate has a molecular weight of 10,000 Da to 2,000,000 Da, encapsulates or stabilizes the technique using encapsulation or encapsulation in a vesicle, This method maximizes the collection capacity or facilitates absorption and penetration into the skin. The method according to any one of claims 1 to 3, which is used as an external preparation for skin to strengthen the skin barrier, and provides excellent moisturizing effect, skin moisture supply effect, water loss reduction effect, water holding capacity increase, skin condition improvement, and the like. As a cosmetic composition, characterized in that it has a technique to strengthen the skin barrier. The skin lotion according to any one of claims 1 to 3, wherein the skin lotion comprises a composition incorporated as an active ingredient for use as an external preparation for skin to improve skin wrinkles and to restore healthy skin and to delay skin aging. Cosmetics such as latex lotion, beauty essence, nutrition cream, sunscreen cream, massage cream, cleansing cream, BB cream, foundation, wrinkle improvement and whitening cream, skin elasticity and moisturizing cream.

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