CN109699180B - 细胞共培养芯片及其制备方法 - Google Patents

细胞共培养芯片及其制备方法 Download PDF

Info

Publication number
CN109699180B
CN109699180B CN201780043057.1A CN201780043057A CN109699180B CN 109699180 B CN109699180 B CN 109699180B CN 201780043057 A CN201780043057 A CN 201780043057A CN 109699180 B CN109699180 B CN 109699180B
Authority
CN
China
Prior art keywords
support
diameter
perforation
protrusion
value
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780043057.1A
Other languages
English (en)
Chinese (zh)
Other versions
CN109699180A (zh
Inventor
唐纳德·基思·马丁
娜塔丽·皮克莱-德哈汉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Grenoble Alpes
Original Assignee
Universite Grenoble Alpes
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite Grenoble Alpes filed Critical Universite Grenoble Alpes
Publication of CN109699180A publication Critical patent/CN109699180A/zh
Application granted granted Critical
Publication of CN109699180B publication Critical patent/CN109699180B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502707—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the manufacture of the container or its components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/02—Membranes; Filters
    • C12M25/04—Membranes; Filters in combination with well or multiwell plates, i.e. culture inserts

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Clinical Laboratory Science (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201780043057.1A 2016-05-11 2017-05-11 细胞共培养芯片及其制备方法 Active CN109699180B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR16/54213 2016-05-11
FR1654213A FR3051195B1 (fr) 2016-05-11 2016-05-11 Puce de co-culture cellulaire et son procede de fabrication
PCT/FR2017/051148 WO2017194894A1 (fr) 2016-05-11 2017-05-11 Puce de co-culture cellulaire et son procede de fabrication

Publications (2)

Publication Number Publication Date
CN109699180A CN109699180A (zh) 2019-04-30
CN109699180B true CN109699180B (zh) 2022-07-26

Family

ID=56855554

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780043057.1A Active CN109699180B (zh) 2016-05-11 2017-05-11 细胞共培养芯片及其制备方法

Country Status (6)

Country Link
US (1) US11857958B2 (enExample)
EP (1) EP3455342B1 (enExample)
JP (1) JP6983180B2 (enExample)
CN (1) CN109699180B (enExample)
FR (1) FR3051195B1 (enExample)
WO (1) WO2017194894A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20230098968A1 (en) * 2018-05-25 2023-03-30 The University Of North Carolina At Chapel Hill Formation of arrays of planar intestinal crypts possessing a stem/proliferative cell compartment and differentiated cell zone
JP2022511375A (ja) 2018-11-16 2022-01-31 ザ ユニバーシティ オブ ノース カロライナ アット チャペル ヒル インビトロ細胞培養粘液系
CN113820189A (zh) * 2021-09-10 2021-12-21 深圳市森盈生物科技有限公司 一种用于非妇科的肿瘤筛查的细胞芯片的制备方法

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004065087A (ja) * 2002-08-06 2004-03-04 National Institute For Materials Science 高密度培養が可能な細胞培養体とその培養モジュール
US7494811B2 (en) * 2003-05-01 2009-02-24 Lifenet Health In vitro growth of tissues suitable to the formation of bone and bone forming tissue formed thereby
JP2005143343A (ja) 2003-11-12 2005-06-09 Jgc Corp 細胞培養装置および細胞培養方法
DK2138233T3 (da) * 2008-06-02 2011-01-31 Boehringer Ingelheim Micropart Mikrofluid foliestruktur til dosering af væsker
KR102140055B1 (ko) 2008-07-16 2020-07-31 칠드런'즈 메디컬 센터 코포레이션 마이크로채널을 갖는 기관 모방 장치 및 그 사용 및 제조 방법
JP2012509663A (ja) * 2008-11-21 2012-04-26 コーニング インコーポレイテッド 細胞培養のための、突起間隔を開けた基板および装置
US8748180B2 (en) * 2009-07-29 2014-06-10 Cornell University Microfluidic device for pharmacokinetic-pharmacodynamic study of drugs and uses thereof
FR2955852B1 (fr) * 2010-01-29 2015-09-18 Corning Inc Dispositif microfluideique en verre, ceramique ou vitroceramique, comprenant une couche intermediaire de traitement comprenant au moins une face ayant une surface structuree ouverte definissant un microcanal ferme par une couche formant feuille en verre, ceramique ou vitroceramique essentiellement plane
JP5700460B2 (ja) 2010-09-10 2015-04-15 株式会社島津製作所 細胞培養デバイス及び細胞培養方法
CA2828110C (en) * 2011-02-28 2020-03-31 President And Fellows Of Harvard College Cell culture system
WO2014008358A1 (en) * 2012-07-05 2014-01-09 Cornell University Porous membrane apparatus, method, and applications
CN104830683B (zh) * 2015-04-30 2017-06-30 大连医科大学附属第二医院 一种用于模拟体内肿瘤细胞及其转移微环境的仿生微流控芯片

Also Published As

Publication number Publication date
JP6983180B2 (ja) 2021-12-17
CN109699180A (zh) 2019-04-30
JP2019518443A (ja) 2019-07-04
FR3051195B1 (fr) 2018-05-18
EP3455342B1 (fr) 2023-10-25
US11857958B2 (en) 2024-01-02
WO2017194894A1 (fr) 2017-11-16
EP3455342A1 (fr) 2019-03-20
US20190168216A1 (en) 2019-06-06
FR3051195A1 (fr) 2017-11-17

Similar Documents

Publication Publication Date Title
Shin et al. 3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert
US20240076595A1 (en) Devices for simulating a function of a tissue and methods of use and manufacturing thereof
US20220403313A1 (en) Open-top microfluidic devices and methods for simulating a function of a tissue
Corral-Nájera et al. Polymeric and biological membranes for organ-on-a-chip devices
JP7588582B2 (ja) 細胞内において又は細胞によって誘導された機械的歪みの評価のための装置
Stucki et al. A lung-on-a-chip array with an integrated bio-inspired respiration mechanism
US20020173033A1 (en) Device and method or three-dimensional spatial localization and functional interconnection of different types of cells
Chung et al. Surface-treatment-induced three-dimensional capillary morphogenesis in a microfluidic platform
Hosic et al. Rapid prototyping of multilayer microphysiological systems
Taebnia et al. Dual-material 3D-printed intestinal model devices with integrated villi-like scaffolds
WO2004046337A2 (en) Multilayered microcultures
US11643625B2 (en) Hydrogel-based organ-on-chip microfluidic device
CN109699180B (zh) 细胞共培养芯片及其制备方法
CN107532134A (zh) 用于三维细胞和/或组织构建体的产生和/或培养和/或成熟的微流体设备和相关方法
CN117813186A (zh) 一种形成微流体凝胶结构的方法和装置
US12180446B2 (en) Native extracellular matrix-derived membrane inserts for organs-on-chips, multilayer microfluidics microdevices, bioreactors, tissue culture inserts, and two-dimensional and three-dimensional cell culture systems
US11268961B2 (en) Method for diagnosing genitourinary cancers
She et al. Fabrication of sac-like hydrogel membranes for replicating curved tissue barriers on chips
US20250388871A1 (en) Extracellular matrix (ecm)-embedded vascular channel-on-chip, airway-on-a-chip and methods of making same
GB2588276A (en) Devices for simulating a function of a tissue and methods of use and manufacturing thereof
Weber et al. Microengineered alveolar array lung-on-chip with hydrogel membrane and simulated breathing mechanics for anti-fibrotic testing
Howard Analyzing the Effects of Scaffold and Synthesized Material Properties in an in vitro Colonic Epithelial Cell Model
EP4326442A1 (en) Biomimetic joint on a chip
Bhadriraju et al. Engineering Cell Adhesion
Liang HEPATOCYTE POLARITY AND FUNCTION ENHANCEMENT THROUGH SCALABLE COMPACTION

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20220525

Address after: The French Saint horse tankard eihe

Applicant after: UNIVERSITE GRENOBLE ALPES

Address before: The French Saint horse tankard eihe

Applicant before: UNIVERSITE GRENOBLE ALPES

Applicant before: COMMISSARIAT A L'ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES

GR01 Patent grant
GR01 Patent grant