CN109673505A - A kind of cross breeding method of industrial hemp - Google Patents
A kind of cross breeding method of industrial hemp Download PDFInfo
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- CN109673505A CN109673505A CN201910129138.4A CN201910129138A CN109673505A CN 109673505 A CN109673505 A CN 109673505A CN 201910129138 A CN201910129138 A CN 201910129138A CN 109673505 A CN109673505 A CN 109673505A
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- dmg240
- dmg231
- male parent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- Life Sciences & Earth Sciences (AREA)
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- Genetics & Genomics (AREA)
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- Developmental Biology & Embryology (AREA)
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The present invention relates to crossbreeding technology field, in particular to a kind of cross breeding method of industrial hemp.The cross breeding method includes the following steps: to sow maternal DMG231, sowing male parent DMG240；Maternal DMG231 and male parent DMG240 interlacing are planted；Maternal DMG231 hybridizes with male parent DMG240, obtains F1 generation seed；F1 generation seed is planted, F1 generation plant is obtained.The high cannabidiol industrial hemp cenospecies of the allfemale type being bred as through the invention has the characteristics of CBD content is high, all females of gender, suitable southern region of China plantation.
Description
Technical field
The present invention relates to crossbreeding technology field, in particular to a kind of cross breeding method of industrial hemp.
Background technique
Hemp is 1 year raw herbaceous plant of Cannabaceae (Cannabinaceae) Cannabis (Cannabis).Industrial hemp refers to
Tetrahydrocannabinol content is lower than 0.3% hemp, and the also referred to as Chinese is numb (hemp), can be used as fine, seed use, medicinal etc..It is now domestic
Outer widely applied hemp is industrial hemp.
Industrial hemp research is hot spot in recent years, and especially medicinal industrial hemp research is in the ascendant.Cannabis plants institute
In more than the 130 kinds of cannboids contained, cannabidiol (CBD) is important always because of its non-additive and huge medical value
Medicine based raw material.Clinical studies show CBD has the function of powerful anti-inflammatory, anticonvulsion, antitumor and resisting mental disease.CBD is main
It is present in the floral leaf of hemp female plant, the floral leaf of 15-20 centimetres of top of female plant inflorescence part is mainly harvested in production.
Hemp is a kind of short-day plant, enters reproductive growth by nutrient growth and is mainly determined by the length of sunshine-duration.
The hemp for originating in the north, introduces a fine variety to after south, and 30-50 centimetres of plant height when enters the florescence, and plant is short and small, and inflorescence is short,
Biological yield is very low；Southern hemp is originated in, is introduced a fine variety to after the north, vegetative growth phase extends, and florescence, shadow are just entered after frost
Reaching maturity for flower and seed is rung, biological yield and CBD content are influenced.Therefore, the hemp in separate sources area, plantation adapt to
Region is extremely limited.
Therefore, research and development are suitable for the high CBD of specific planting area, allfemale type Hemp Varieties, are that medicinal industrial hemp is promoted to produce
Industry is promoted and the key of development.
Summary of the invention
In view of this, the present invention provides a kind of cross breeding methods of industrial hemp.The hybridization that the hybridizing method obtains
Plant has many advantages, such as high CBD, allfemale type.
In order to achieve the above-mentioned object of the invention, the present invention the following technical schemes are provided:
The present invention provides a kind of cross breeding methods of industrial hemp, include the following steps:
Female parent DMG231 is sowed, male parent DMG240 is sowed；Maternal DMG231 and male parent DMG240 interlacing are planted；
Maternal DMG231 hybridizes with male parent DMG240, obtains F1 generation seed, i.e. numb No. 1 seed of the Chinese.
The method of the present invention is first using monoecism industrial hemp DMG240 as male parent, with high cannabidiol (CBD) content
Dioecism industrial hemp DMG231 is female parent, and preparing hybrid combination, obtained F1 generation is the high cannabidiol of allfemale type (CBD)
Industrial hemp cenospecies is named as Chinese fiber crops No. 1.The high cannabidiol of the allfemale type being bred as through the invention (CBD) industrial hemp is miscellaneous
Kind is handed over, there is the characteristics of CBD content is high, all females of gender, suitable southern region of China plantation.
One of key of the invention is: Parent, which should stagger the time, to be sowed so that its florescence is consistent.Preferably, maternal
The date of seeding of DMG231 15~45 days more early than the date of seeding of male parent DMG240.
Preferably, 40~45 days more early than the date of seeding of male parent DMG240 of the date of seeding of maternal DMG231.
Preferably, the date of seeding of female parent DMG231 is late March to early April.
Preferably, the date of seeding of maternal DMG231 is early April.
In specific embodiment provided by the invention, the date of seeding of maternal DMG231 is April 1.
Preferably, the date of seeding of male parent DMG240 is the first tenday period of a month in May to mid-May.
Preferably, the date of seeding of male parent DMG240 is mid-May.
In specific embodiment provided by the invention, the date of seeding of male parent DMG240 is May 15.
Preferably, the plant quantitative proportion of male parent DMG240 and female parent DMG231 are 1:(1~3 when interlacing is planted).
Preferably, when interlacing is planted, the plant quantitative proportion of male parent DMG240 and female parent DMG231 are 1:1.
One of another key of the invention is: male parent DMG240 should carry out impurity elimination, and maternal DMG231 should carry out emasculation.
Preferably, interlacing plantation with hybridize between further include: after male parent DMG240 enters the florescence, pull out hybrid strain, weak strain.
Preferably, interlacing plantation with hybridize between further include: after maternal DMG231 enters the florescence, pull out all staminiferous plants.
Preferably, after obtaining F1 generation seed further include: plantation F1 generation seed obtains F1 generation plant.
The present invention provides a kind of cross breeding methods of industrial hemp.The cross breeding method includes the following steps: to broadcast
Kind female parent DMG231, sows male parent DMG240；Maternal DMG231 and male parent DMG240 interlacing are planted；Maternal DMG231 and male parent
DMG240 hybridization, obtains F1 generation seed；F1 generation seed is planted, F1 generation plant is obtained.The technical effect that the present invention has are as follows:
(1) under the premise of controlling male parent impurity elimination, in the F1 generation hybrid that the present invention obtains, female plant ratio is up to 95%-
99.9%.CBD is mainly extracted from female plant, and therefore, full female plant industrial hemp economic value, which is much larger than, contains certain proportion staminiferous plant
Material.
(2) the F1 hybrid that the present invention obtains, it (is Yunnan No. 7 CBD of main breed cloud fiber crops that the CBD content that is averaged, which is 3.60%,
3-4 times of content), average THC content is 0.18%, and plant height is about 1.6-2.0 meters, and therefore, this strain planting benefit is considerable.
Specific embodiment
The invention discloses a kind of cross breeding method of industrial hemp, those skilled in the art can be used for reference in this paper
Hold, is suitably modified realization of process parameters.In particular, it should be pointed out that all similar substitutions and modifications are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.Method and application of the invention, which has passed through, preferably to be implemented
Example is described, related personnel obviously can not depart from the content of present invention, in spirit and scope to method described herein and
Using being modified or appropriate changes and combinations, carry out implementation and application the technology of the present invention.
Vegetable material of the present invention includes:
1) hermaphrodite male parent industrial hemp DMG240 is a kind of precocious type industrial hemp strain, breeding time about 135
It, source place is Chinese Heilungkiang.
2) dioecious female parent industrial hemp DMG231 is a kind of late-maturing type industrial hemp strain, breeding time about 165 days,
Source place is Chinese yunnan.It is the elite clone that characterization and evaluation goes out from the collection of national crudefiber crop germplasm mid-term library.
It carries out in accordance with the following steps:
(1) field is maternal by the sowing of industrial hemp general planting method, plantation: because Parent source place latitude difference compared with
Greatly, different from required time of blooming is seeded into, it therefore, be according to the weather and sunshine feature on production of hybrid seeds ground, reasonable arrangement Parent
Sowing time, carry out field planting management appropriate, keep the florescence of Parent consistent.In general, female parent should compare male parent
Early sowing 15-45 days.
(2) field is by the sowing of industrial hemp general planting method, plantation male parent；
(3) it to ensure that the seed quality and purity of Parent and its hybrid reach the requirement of national relevant regulations, realizes miscellaneous
The large-scale production for handing over seed, after male parent enters the florescence, will pull out hybrid strain, weak strain.After female parent enters the florescence, all heros are pulled out
Strain.
(4) obtained hybridization industrial hemp seed is planted, can be directly used for industrial hemp production application, because hybridizing F1 seed
For full female plant, the economic benefit of industrial hemp plantation can be improved, also can effectively prevent elite clone loss.
(5) the first familiar generation industrial hemp obtained is named as Chinese fiber crops No. 1.The Chinese No. 1 heredity of fiber crops good spy of Parent
Property, average CBD content is 3.60%, and average THC content is 0.18%, and in southern area of China, plantation plant height is up to 1.6~2
Rice.
Seed variety used can be provided by national crudefiber crop germplasm in the cross breeding method of industrial hemp provided by the invention
Source mid-term library obtains.
Below with reference to embodiment, the present invention is further explained:
Embodiment 1
It is maternal: DMG231；
Male parent: DMG240.
The implantation methods of industrial hemp are as follows in the present embodiment:
(1) field is maternal by the sowing of industrial hemp general planting method, plantation: sowing on April 1st, 2018 is maternal.
(2) field is by the sowing of industrial hemp general planting method, plantation male parent；Sowing on May 15th, 2018 male parent.
(3) Parent is planted with the ratio interlacing of 1:1, after male parent enters the florescence, to pull out hybrid strain, weak strain.Female parent enters
After florescence, all staminiferous plants are pulled out.
(4) obtained hybridization industrial hemp seed is planted, female plant ratio is 98%, is planted in Changsha, Hunan, average
CBD content is 3.60%, and average THC content is 0.18%, and average plant height is 1.6~2 meters.
1 CBD comparision contents of test example
CBD content detection result is as follows:
Table 1
Contained by above-mentioned test result it is found that hybridizing CBD in the F1 plant obtained with male parent DMG240 by female parent DMG231
Amount is significantly higher than other kinds.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (6)
1. a kind of cross breeding method of industrial hemp, which comprises the steps of:
Female parent DMG231 is sowed, male parent DMG240 is sowed；The female parent DMG231 and the male parent DMG240 interlacing are planted；
The female parent DMG231 hybridizes with the male parent DMG240, obtains F1 generation seed, i.e. numb No. 1 seed of the Chinese.
2. cross breeding method according to claim 1, which is characterized in that the date of seeding of the female parent DMG231 compares male parent
The date of seeding of DMG240, is 15~45 days early.
3. cross breeding method according to claim 1, which is characterized in that interlacing plant when, the male parent DMG240 with
The plant quantitative proportion of the female parent DMG231 is 1:(1~3).
4. cross breeding method according to claim 1, which is characterized in that the interlacing plantation is gone back between described hybridize
It include: to pull out hybrid strain, weak strain after male parent DMG240 enters the florescence.
5. cross breeding method according to claim 1, which is characterized in that the interlacing plantation is gone back between described hybridize
It include: after maternal DMG231 enters the florescence, to pull out all staminiferous plants.
6. cross breeding method according to claim 1, which is characterized in that after obtaining F1 generation seed further include: plantation
F1 generation seed obtains F1 generation plant.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910129138.4A CN109673505B (en) | 2019-02-21 | 2019-02-21 | Industrial marihuana crossbreeding method |
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| CN201910129138.4A CN109673505B (en) | 2019-02-21 | 2019-02-21 | Industrial marihuana crossbreeding method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110419397A (en) * | 2019-09-06 | 2019-11-08 | 黑龙江汉美生工业大麻科技有限公司 | A kind of Cultivate administration method improving No. 5 CBD contents of imperial hemp |
| CN111034546A (en) * | 2019-12-23 | 2020-04-21 | 云南素麻生物科技有限公司 | Breeding method of industrial cannabis with high CBD content |
| CN111742838A (en) * | 2020-07-17 | 2020-10-09 | 黑龙江润地汉麻科技有限公司 | Artificial pollination method for medical industrial hemp |
| CN111758557A (en) * | 2020-07-20 | 2020-10-13 | 黑龙江省科学院大庆分院 | Industrial hemp hybrid pollination method for male and female heterozygotic seeds |
| CN112921114A (en) * | 2021-04-14 | 2021-06-08 | 中国农业科学院麻类研究所 | DNA sequence marker for identifying hemp plant sex and detection primer thereof |
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| CN109122306A (en) * | 2018-08-20 | 2019-01-04 | 杭州昶胜电子商务有限公司 | A method of obtaining high cannabidiol content industrial hemp plant |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109122306A (en) * | 2018-08-20 | 2019-01-04 | 杭州昶胜电子商务有限公司 | A method of obtaining high cannabidiol content industrial hemp plant |
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| 张建春等: "《汉麻籽综合利用加工技术》", 31 December 2010, 中国轻工业出版社 * |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110419397A (en) * | 2019-09-06 | 2019-11-08 | 黑龙江汉美生工业大麻科技有限公司 | A kind of Cultivate administration method improving No. 5 CBD contents of imperial hemp |
| CN111034546A (en) * | 2019-12-23 | 2020-04-21 | 云南素麻生物科技有限公司 | Breeding method of industrial cannabis with high CBD content |
| CN111742838A (en) * | 2020-07-17 | 2020-10-09 | 黑龙江润地汉麻科技有限公司 | Artificial pollination method for medical industrial hemp |
| CN111758557A (en) * | 2020-07-20 | 2020-10-13 | 黑龙江省科学院大庆分院 | Industrial hemp hybrid pollination method for male and female heterozygotic seeds |
| CN112921114A (en) * | 2021-04-14 | 2021-06-08 | 中国农业科学院麻类研究所 | DNA sequence marker for identifying hemp plant sex and detection primer thereof |
| CN112921114B (en) * | 2021-04-14 | 2022-02-15 | 中国农业科学院麻类研究所 | DNA sequence marker for identifying hemp plant sex and detection primer thereof |
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