CN109633125A - A kind of analog study method and apparatus that soil animal forms humic substance - Google Patents
A kind of analog study method and apparatus that soil animal forms humic substance Download PDFInfo
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- CN109633125A CN109633125A CN201811629374.4A CN201811629374A CN109633125A CN 109633125 A CN109633125 A CN 109633125A CN 201811629374 A CN201811629374 A CN 201811629374A CN 109633125 A CN109633125 A CN 109633125A
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Abstract
The invention discloses a kind of analog study methods that soil animal forms humic substance, the present invention is to soil animal, for example the various parameters of earthworm etc. carry out real-time monitoring and control, experimental box is arranged to several cells, the experiment of different parameters can be done simultaneously, convenient for carrying out free segmentation to different research, improve the reliability of research and the science of orthogonal test, simultaneously, what the present invention can be convenient is sampled, and original situation of animal will not be destroyed when sampling, the present invention combines the trend of current international STUDY ON SPATIAL SOIL ECOLOGY development, soil animal can effectively be carried out to form Farmland Black Soil humic substance, the Influencing Mechanism of structure feature is studied, be conducive to abundant and improve soil humic substances formation mechenism theory, the biological effect mechanism formed for deep announcement soil humic substances, formulate suitable soil pipe Reason measure is to improve soil productivity and ensure that increases in grain production, promotion black earth region ecology safety have important scientific meaning.
Description
Technical field
The present invention relates to the analog study method and apparatus that a kind of soil animal forms humic substance, belong to soil animal
Technical field.
Background technique
Earthworm is one of the most typical soil macro-invertebrate being distributed widely in terrestrial ecosystems, and biomass is big, quilt
Referred to as " ecological systems engineering teacher ".Earthworm can significantly improve soil by activities such as feeding, excretion, secretion mucus, excavated cavities
Earth structure improves soil aeration water permeable ability and fertility, influences soil material circulation and energy flow.Earthworm passes through feeding, pick
Cave, itself metabolism-breathing, secretion and excretion, directly affect the formation and distribution of soil agreegate and humic substance.Together
When, the activity of earthworm changes the composition of soil texture, carbon nitrogen composition and edaphon and other soil animals, influences soil indirectly
Earth humic substance is formed.
Currently, research both domestic and external is concentrated mainly on soil animal to the shadow of Decomposition of leaf litter and Organic Matter Transformation etc.
It rings, is seldom related to the effect that soil animal forms humic substance and converts, and soil animal is to three of black earth humic substance
Component humic acid, fulvic acid and humin are formed and the mechanism of action of conversion is not clear, moreover, Different groups soil animal pair
Black earth humic substance is formed and conversion is closed with the presence or absence of difference, with the presence or absence of interaction, soil animal and black earth humic substance
The problems such as being it is also highly desirable to have solution.
The present invention is in view of the above problems, providing a kind of analog study method that soil animal forms humic substance and filling
It sets, to combine the trend of current international STUDY ON SPATIAL SOIL ECOLOGY development, carries out soil animal to Farmland Black Soil humic substance shape
At the Influencing Mechanism research of, structure feature, is conducive to abundant and improves soil humic substances formation mechenism theory, for deeply taking off
Show the biological effect mechanism that soil humic substances is formed, formulates suitable Soil suppressiveness to improve soil productivity and ensure grain
Food volume increase promotes black earth region ecology safety to have important scientific meaning.
Summary of the invention
To achieve the above object, the invention provides the following technical scheme: the mould that a kind of soil animal forms humic substance
Quasi- research device, which is characterized in that it includes experimental box body, outer incubator, interior incubator, vertical stand, horizontal bracket, hydrojet group
Part, ventilation temperature control assembly and sampling assemble, wherein the intracorporal middle part of experimental box is fixedly installed vertical stand, described
Experimental box body is divided into left and right chamber by vertical stand, be provided in the vertical stand it is multiple between the upper and lower every and horizontal extension arrangement
Multiple horizontal brackets, the fixed support of outer incubator are arranged on the horizontal bracket, and the both ends of the experimental box body are all provided with
It is equipped with experiment chamber door, the lower end of the experiment chamber door is hingedly arranged on the experimental box body, is provided on the experiment chamber door
It can be passed through the ventilation connector with certain temperature air, the ventilation connector is connect with ventilation temperature control assembly, described perpendicular
Straight frame, experimental box body inner wall and experiment cover wall are provided with the corresponding fastening seal groove in position, the fastening seal groove
The interior seal buckle that can be dismantled is equipped with multiple groups insulation board, and experimental box body is divided into multiple spaces by the insulation board, in order to grind
The different parameters for studying carefully different spaces control lower soil animal to humic substance formational situation, and by selection installation different number and
The insulation board of position tests analog case needed for meeting, and is provided with interior incubator, the interior incubator in the outer incubator
It is inside provided with humic substance, the inner upper of the interior incubator is additionally provided with hydrojet component, the close institute of the interior incubator
The side for stating experiment chamber door is additionally provided with sampling assemble, and the sampling assemble can be sampled humic substance, in order to right
The ingredient of humic substance is researched and analysed.
Further, preferably, the hydrojet component includes hydrojet supervisor, hydrojet branch pipe and branch pipe control valve, wherein institute
It states hydrojet supervisor to be laid in the vertical stand, the hydrojet branch pipe is connected to the hydrojet and is responsible for, on the hydrojet branch pipe
Jet pipe protrudes into the interior incubator, and is provided with branch pipe control valve on the hydrojet branch pipe, so as to in each interior incubator
The case where individually controlled.
Further, preferably, the sampling assemble is the multiple groups of multiple rows of multiple row level, every group of sampling assemble protrudes into institute
It states in interior incubator, the sampling assemble includes outer lever, sampling frame and sampling egative film, wherein one end of the sampling frame
It is fixedly connected with the outer lever, the lower end of the outer lever is provided with through slot, and the sampling egative film can be by described logical
Slot protrudes into, and the sampling egative film protrudes into the rear whole lower end surface for being close to the sampling frame well, is provided on the sampling frame multiple
Using the spaced apart hole frame of partition, humic substance to be collected is contained in the hole frame.
Further, it preferably, the partition is in tilted layout, and is tilted to the side far from experiment chamber door.
Further, preferably, the ventilation temperature control assembly is the ventilation of gases device of constant temperature.
Further, preferably, the material that the interior incubator and outer incubator are porous breathable structure is made.
Further, preferably, the side of the close experiment chamber door of the outer incubator is provided with lower end and is hinged on outer training
The outer chamber door on case is supported, the setting that the interior incubator can skid off by the way of sliding block and sliding slot cooperation is in the outer incubator
It is interior.
Further, preferably, the interior incubator is internally provided with temperature sensor, humidity sensor, pH value sensing
Device, the temperature sensor, humidity sensor, pH value sensor are connect with controller, and the controller controls the device
The movement of various components.
Further, described interior preferably, being provided with the interior culture case lid detachably fastened at the top of the interior incubator
The inner wall of culture case lid is provided with engaging locating piece, and the top cooperation of the engaging locating piece and the interior incubator is described interior
Mini fan is provided between the bottom and ventilating board of incubator, the bottom of the interior incubator is located at the position of the mini fan
It installs and is equipped with inner vent, the bottom of the humic substance layer is provided with spongy layer, and the bottom of the spongy layer is using described logical
Aerofoil support.
Further, the present invention also provides a kind of analog study method that soil animal forms humic substance, features
Be: itself the following steps are included:
(1) according to the sample number of required research, the space number that experimental box body is divided by insulation board is determined, in order to study
The different parameters of different spaces control lower soil animal to humic substance formational situation, for example Different groups soil animal is to black earth
Humic substance is formed between transforming relationship situation, Different groups soil animal with the presence or absence of interaction, soil animal and black
Relationship etc. between native humic substance;
(2) according to space number, each ventilation temperature control assembly is connected, so that the temperature in each space reaches ventilation temperature
The control temperature for spending control assembly, i.e., so that interior incubator and outer incubator reach required temperature condition;
(3) interior incubator is skidded off, by the good humic substance to be studied of its internal addition, and is packed into soil animal, then,
It is loaded into outer incubator;
(4) culture a period of time takes out a certain amount of humic substance using sampling assemble, carries out analysis to its ingredient and grinds
Study carefully, such as to component humic acid, fulvic acid, humin, nitrogen content, the phosphorus content etc. in humic substance, then, using one
After the section time, periodically takes a sample to research, analog study result can be obtained.
Compared with prior art, the beneficial effects of the present invention are:
The present invention carries out real-time monitoring and control to the various parameters of soil animal, such as earthworm etc., and experimental box is arranged
At several cells, the experiment of different parameters can be done simultaneously, convenient for carrying out free segmentation to different research, improve research
The science of reliability and orthogonal test, meanwhile, what the present invention can be convenient is sampled, and will not destroy animal when sampling
Original situation, the present invention combines the trend of current international STUDY ON SPATIAL SOIL ECOLOGY development, can effectively carry out soil animal
Farmland Black Soil humic substance is formed, the research of the Influencing Mechanism of structure feature, is conducive to abundant and improves soil humic substances shape
Suitable Soil suppressiveness is formulated for the biological effect mechanism that deep announcement soil humic substances is formed at mechanism theory
To improve soil productivity and ensure that increases in grain production, promotion black earth region ecology safety have important scientific meaning.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram for the analog study device that soil animal forms humic substance of the present invention;
Fig. 2 is a kind of sampling assemble structural representation for the analog study device that soil animal forms humic substance of the present invention
Figure;
The interior incubator for the analog study device that Fig. 3, which is a kind of soil animal of the present invention, forms humic substance and outer culture
Case schematic diagram of internal structure;
Specific embodiment
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
Please refer to Fig. 1-3, the present invention provides a kind of technical solution: a kind of simulation that soil animal forms humic substance is ground
Study carefully device, which is characterized in that it includes experimental box body 1, outer incubator 8, interior incubator 9, vertical stand 2, horizontal bracket 3, hydrojet
Component, ventilation temperature control assembly and sampling assemble 13, wherein the middle part in the experimental box body 1 is fixedly installed vertical stand
2, experimental box body is divided into left and right chamber by the vertical stand 2, be provided in the vertical stand 2 it is multiple between the upper and lower every and level prolong
Multiple horizontal brackets 3 of arrangement are stretched, the fixed support of outer incubator 8 is arranged on the horizontal bracket 3, the experimental box body
1 both ends are provided with experiment chamber door 5, and the lower end of the experiment chamber door 5 is hingedly arranged on the experimental box body, the experiment
The ventilation connector 11 with certain temperature air can be passed through by being provided on chamber door 5, the ventilation connector 11 and ventilation temperature control
Component connection processed, the vertical stand, experimental box body inner wall and experiment cover wall are provided with the corresponding fastening sealing in position
Slot, the seal buckle that can be dismantled in seal groove that fastens are equipped with multiple groups insulation board 4, and the insulation board 4 divides experimental box body
At multiple spaces, lower soil animal is controlled to humic substance formational situation in order to study the different parameters of different spaces, and logical
The insulation board of selection installation different number and position is crossed to meet required experiment analog case, is provided in the outer incubator 8
Interior incubator 9 is provided with humic substance in the interior incubator 9, and the inner upper of the interior incubator is additionally provided with hydrojet group
The side close to the experiment chamber door of part, the interior incubator is additionally provided with sampling assemble 13, and the sampling assemble 13 can
Humic substance is sampled, in order to which the ingredient to humic substance is researched and analysed.
In the present embodiment, the hydrojet component includes hydrojet supervisor 7, hydrojet branch pipe 12 and branch pipe control valve 6, wherein
The hydrojet supervisor 7 is laid in the vertical stand 2, and the hydrojet branch pipe 12 is connected to the hydrojet supervisor 7, the hydrojet branch
Jet pipe 22 on pipe protrudes into the interior incubator, and is provided with branch pipe control valve 6 on the hydrojet branch pipe, so as to each interior
Situation in incubator is individually controlled.
The sampling assemble 13 is the multiple groups of multiple rows of multiple row level, and every group of sampling assemble 13 protrudes into the interior incubator
Interior, the sampling assemble includes outer lever 14, sampling frame 17 and sampling egative film 16, wherein one end of the sampling frame 17 is solid
Surely the outer lever 14 is connected, the lower end of the outer lever 14 is provided with through slot 15, and the sampling egative film 16 can pass through
The through slot 15 protrudes into, and the sampling egative film protrudes into the rear whole lower end surface for being close to the sampling frame well, on the sampling frame 17
Multiple use spaced apart hole frames 18 of partition are provided with, humic substance to be collected is contained in the hole frame 18.
What sample can be intact when in order to realize that sampling assemble is extracted out is located in the hole frame 18, and the partition tilts cloth
It sets, and is tilted to the side far from experiment chamber door.
In the present embodiment, the ventilation temperature control assembly is the ventilation of gases device of constant temperature.The interior incubator and outer
Incubator is that the material of porous breathable structure is made.
The side of the close experiment chamber door of the outer incubator is provided with the outer chamber door 20 that lower end is hinged on outer incubator,
The setting that the interior incubator can skid off in such a way that sliding block 26 and sliding slot 27 cooperate is in the outer incubator 8.In described
Incubator is internally provided with temperature sensor 25, humidity sensor, pH value sensor, the temperature sensor, humidity sensor,
PH value sensor is connect with controller, and the controller controls the movement of the various components of the device.
The interior culture case lid 23 detachably fastened, the inner wall of the interior culture case lid are provided at the top of the interior incubator
It is provided with engaging locating piece 21, the top cooperation of the engaging locating piece and the interior incubator, the bottom of the interior incubator
Mini fan 28 is provided between ventilating board, the position that the bottom 19 of the interior incubator is located at the mini fan is provided with
Inner vent, the bottom of the humic substance layer 24 are provided with spongy layer, and the bottom of the spongy layer uses the ventilating board branch
Support.
In addition, the present invention also provides a kind of analog study method that soil animal forms humic substance, feature exists
In: itself the following steps are included:
(1) according to the sample number of required research, the space number that experimental box body is divided by insulation board is determined, in order to study
The different parameters of different spaces control lower soil animal to humic substance formational situation, for example Different groups soil animal is to black earth
Humic substance is formed between transforming relationship situation, Different groups soil animal with the presence or absence of interaction, soil animal and black
Relationship etc. between native humic substance;
(2) according to space number, each ventilation temperature control assembly is connected, so that the temperature in each space reaches ventilation temperature
The control temperature for spending control assembly, i.e., so that interior incubator and outer incubator reach required temperature condition;
(3) interior incubator is skidded off, by the good humic substance to be studied of its internal addition, and is packed into soil animal, then,
It is loaded into outer incubator;
(4) culture a period of time takes out a certain amount of humic substance using sampling assemble, carries out analysis to its ingredient and grinds
Study carefully, such as to component humic acid, fulvic acid, humin, nitrogen content, the phosphorus content etc. in humic substance, then, using one
After the section time, periodically takes a sample to research, analog study result can be obtained.
The present invention carries out real-time monitoring and control to the various parameters of soil animal, such as earthworm etc., and experimental box is arranged
At several cells, the experiment of different parameters can be done simultaneously, convenient for carrying out free segmentation to different research, improve research
The science of reliability and orthogonal test, meanwhile, what the present invention can be convenient is sampled, and will not destroy animal when sampling
Original situation, the present invention combines the trend of current international STUDY ON SPATIAL SOIL ECOLOGY development, can effectively carry out soil animal
Farmland Black Soil humic substance is formed, the research of the Influencing Mechanism of structure feature, is conducive to abundant and improves soil humic substances shape
Suitable Soil suppressiveness is formulated for the biological effect mechanism that deep announcement soil humic substances is formed at mechanism theory
To improve soil productivity and ensure that increases in grain production, promotion black earth region ecology safety have important scientific meaning.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (10)
1. the analog study device that a kind of soil animal forms humic substance, which is characterized in that it includes experimental box body, outer training
Support case, interior incubator, vertical stand, horizontal bracket, hydrojet component, ventilation temperature control assembly and sampling assemble, wherein the reality
The intracorporal middle part of tryoff is fixedly installed vertical stand, and experimental box body is divided into left and right chamber, the vertical stand by the vertical stand
On be provided with it is multiple between the upper and lower every and horizontal extension arrangement multiple horizontal brackets, the fixed support of the outer incubator is arranged in institute
It states on horizontal bracket, the both ends of the experimental box body are provided with experiment chamber door, and the lower end of the experiment chamber door is hingedly arranged in
On the experimental box body, the ventilation connector with certain temperature air can be passed through by being provided on the experiment chamber door, described logical
Wind connector is connect with ventilation temperature control assembly, and the vertical stand, experimental box body inner wall and experiment cover wall are provided with
The corresponding fastening seal groove in position, the seal buckle that can be dismantled in seal groove that fastens are equipped with multiple groups insulation board, the heat preservation
Experimental box body is divided into multiple spaces by plate, controls lower soil animal to Humus in order to study the different parameters of different spaces
Matter formational situation, and analog case is tested needed for meeting by selection installation different number and the insulation board of position, it is described outer
It is provided with interior incubator in incubator, humic substance is provided in the interior incubator, the inner upper of the interior incubator is also
It is provided with hydrojet component, the side close to the experiment chamber door of the interior incubator is additionally provided with sampling assemble, the sampling
Component can be sampled humic substance, in order to which the ingredient to humic substance is researched and analysed.
2. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The hydrojet component includes hydrojet supervisor, hydrojet branch pipe and branch pipe control valve, wherein it is described vertical that the hydrojet supervisor is laid in
On frame, the hydrojet branch pipe is connected to the hydrojet supervisor, and the jet pipe on the hydrojet branch pipe protrudes into the interior incubator, and
It is provided with branch pipe control valve on the hydrojet branch pipe, individually to be controlled the situation in each interior incubator.
3. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The sampling assemble is the multiple groups of multiple rows of multiple row level, and every group of sampling assemble protrudes into the interior incubator, the sampling group
Part includes outer lever, sampling frame and sampling egative film, wherein one end of the sampling frame is fixedly connected with the outer lever, institute
The lower end for stating outer lever is provided with through slot, and the sampling egative film can be protruded by the through slot, and the sampling egative film is stretched
Enter the rear whole lower end surface for being close to the sampling frame well, multiple use spaced apart hole frames of partition be provided on the sampling frame,
Humic substance to be collected is contained in the hole frame.
4. the analog study device that a kind of soil animal according to claim 3 forms humic substance, it is characterised in that:
The partition is in tilted layout, and tilts to the side far from experiment chamber door.
5. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The ventilation temperature control assembly is the ventilation of gases device of constant temperature.
6. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The interior incubator and outer incubator are that the material of porous breathable structure is made.
7. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The side of the close experiment chamber door of the outer incubator is provided with the outer chamber door that lower end is hinged on outer incubator, the interior culture
The setting that case can be skidded off by the way of sliding block and sliding slot cooperation is in the outer incubator.
8. the analog study device that a kind of soil animal according to claim 1 forms humic substance, it is characterised in that:
The interior incubator is internally provided with temperature sensor, humidity sensor, pH value sensor, and the temperature sensor, humidity pass
Sensor, pH value sensor are connect with controller, and the controller controls the movement of the various components of the device.
9. the analog study method and apparatus that a kind of soil animal according to claim 1 forms humic substance, special
Sign is: the interior culture case lid detachably fastened is provided at the top of the interior incubator, the inner wall of the interior culture case lid is set
It is equipped with engaging locating piece, the top of the engaging locating piece and the interior incubator cooperates, the bottom of the interior incubator and logical
Mini fan is provided between aerofoil, the position that the bottom of the interior incubator is located at the mini fan is provided with interior ventilation
Hole, the bottom of the humic substance layer are provided with spongy layer, and the bottom of the spongy layer is supported using the ventilating board.
10. a kind of analog study method that soil animal forms humic substance, it is characterised in that: it uses claim 1-9
A kind of analog study device that soil animal forms humic substance described in any one comprising following steps:
(1) according to the sample number of required research, the space number that experimental box body is divided by insulation board is determined, in order to study difference
The different parameters in space control lower soil animal to humic substance formational situation;
(2) according to space number, each ventilation temperature control assembly is connected, so that the temperature in each space reaches ventilation temperature control
The control temperature of component processed, i.e., so that interior incubator and outer incubator reach required temperature condition;
(3) interior incubator is skidded off, by the good humic substance to be studied of its internal addition, and soil animal is packed into, then, by it
It is packed into outer incubator;
(4) culture a period of time takes out a certain amount of humic substance using sampling assemble, analyzes and researches to its ingredient, so
Afterwards, it after using a period of time, periodically takes a sample to research, analog study result can be obtained.
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