CN102172178B - Method for testing influence of different carbon dioxide concentrations on growth of submerged plants - Google Patents
Method for testing influence of different carbon dioxide concentrations on growth of submerged plants Download PDFInfo
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- CN102172178B CN102172178B CN 201110072904 CN201110072904A CN102172178B CN 102172178 B CN102172178 B CN 102172178B CN 201110072904 CN201110072904 CN 201110072904 CN 201110072904 A CN201110072904 A CN 201110072904A CN 102172178 B CN102172178 B CN 102172178B
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Abstract
The invention discloses a method for testing the influence of different carbon dioxide concentrations on growth of submerged plants, which is an ecological test method and belongs to the field of ecological test methods. A research station is established beside a natural lake, which collects water and bottom sediment in the lake as test raw materials and performs test research on the influence of multiple groups of different carbon dioxide concentrations on the growth of submerged plants in the condition of ensuring the same growth factors of the submerged plants. In the test, the submerged plants are cultivated with a specialized incubator. The increment of biomass is calculated finally, and the materials are convenient and quick to obtain; the influence of a genetic factor is avoided; the actual growing environment of the submerged plants can be really and reasonably stimulated; and the illumination and temperature conditions of the incubator can be full-manually controlled, thus being favorable for differential control on multiple environmental factors.
Description
Technical field
The present invention relates to a kind of ecological test method, specifically a kind ofly analyze different gas concentration lwevels to the test method of submerged plant growth impact, belong to ecological test method field.
Background technology
Carbonic acid gas is the substrate of photosynthesis of plant, also is an important envirment factor that consists of plant habitat.Along with the development of modern industrial or agricultural and the aggravation of mankind's activity, atmospheric carbon dioxide levels already and will continue to be rising trend.This trend will produce material impact to growth and development of plants, finally also will have influence on human living environment.Submerged plant is the important component part of aquatic ecosystem, has purifying water, increases dissolved oxygen in the water body, suppresses the function of algal grown, and ecosystem material, energy circulation are had the important regulating and controlling effect, is the main Sustainer of healthy aquatic ecosystem.At present relevant gas concentration lwevel rising mainly concentrates on terrestrial plant to the research of plant growth impact, research on the submerged plant impact then seldom relates to, fragmentary relevant report is also carried out in the laboratory, the environment (Xie Yonghong etc., the CO that adopt constant temperature, replenish running water, regulation identical illumination condition every day are simulated submerged plant growth
2Concentration raises on the impact of submerged plant water caltrop leaf phenotype and physiological and biochemical property, Acta Phytoecologica Sinica, 2003), there is a certain distance in artificial submerged plant habitat conditions and the actual conditions of creating in the laboratory, thereby the conclusion that obtains can not reflect accurately, truly that gas concentration lwevel raises to water plants, especially the impact of submerged plant growth is to be worth discussion.Therefore, different gas concentration lwevels still lack more convictive research method to the submerged plant growth impact.
Summary of the invention
Purpose of the present invention just provides the research method that a kind of gas concentration lwevel changes to be affected submerged plant growth.
The technical scheme that the present invention achieves the above object is:
Set up research station on the natural lake limit, the water in the convenient collection lake and bed mud are guaranteeing under the identical condition of the various growth factors of submerged plant as test raw material, are carrying out the different gas concentration lwevel of many groups to the experimental study of submerged plant growth impact.Specifically realize by following steps:
(1) sets up research station on the natural lake limit, in the lake, select a kind of submerged plant, fetch after laboratory cleans up, select the basically identical tender shoots of upgrowth situation, breeding is got with parent and the basically identical tender shoots of upgrowth situation to the first filial generation, places the culture dish that fills nutrient solution, be cultured to the high about 2cm of seedling, select in order to test;
Described nutrient solution is mainly by NH
4NO
3And NaH
2PO
4Form, N, P concentration are respectively 2 and 0.005mg/L, and ion strength and pH are respectively 7.51 and 8.14, and nutrient solution is changed once every day.
(2) the thick sediments of 5cm of packing in 12 incubators, then inject the dark lake water of 20～30cm, sediments is all taken from the lake identical with submerged plant with lake water, after water body leaves standstill 3 days in the incubator, from test material for subsequent use, pick out seedling plantation of the same size in incubator, every case is planted 82 strains, replenishes lake water to the depth of water again and reaches 160cm, should avoid bed mud to suspend when injecting lake water.
(3) 12 incubators are divided into 4 groups, 3 every group, every day, 24h continued to pass into different CO in 4 groups of incubators
2The mist of concentration, culture experiment in lakeside research station, incubation time are 2 months.
The depth of water in the duration of test, incubator all maintains 160cm, CO
2Concentration is by portable CO
2The concentration analyzer monitoring.
(4) during off-test, measure plant growth amount and morphological parameters.Take out the blade of submerged plant, measure length, width and the area of blade, calculate each parameter absolute increment; With blade at 75 ℃ of lower continuous drying 48h to constant, the record biomass; Plant leaf after the oven dry is pulverized, measured biochemical substances and Mineral Concentrations in the blade.All the data specialty mathematical statistics softwares are processed and are analyzed.
Above-mentioned incubator, adopting the length of side is the cube of 200cm, each face all adopts transparent organic glass to make.Sealing adopts four limit packing rings to match the hook-type cover plate, and have contact with on two on every limit, with convenient, flexible keying, possesses simultaneously good obturation effect.The incubator sidewall arranges the carbon dioxide gas mixture access way along highly being provided with graduation mark in relative top side wall, at sidewall the Inlet and outlet water passage is set, and wherein intake tunnel can automatic water supplement in process of the test.
Above-mentioned incubator bed mud top arranges one blind patch, and part is isolated bed mud and water body, and disturbance bed mud when keeping away free-changing water makes plant by this blind patch growth be arranged simultaneously.
Above-mentioned incubator top arranges artificial sun's chromatogram cold light source.
Four jiaos of above-mentioned incubator arrange electro-thermostat below the water surface, i.e. air-conditioning under water.
Because under natural situation, the origin of heat of water bodies of rivers and lakes mainly from the thermal radiation of the sun, if simulation pure natural state should install artificial sun's chromatogram thermal light source additional, provides thermal effect when photosynthetic illumination is provided.But through inventor's test of many times, find that photosynthesis needs in full Artificial Control process illuminance and temperature control are difficult to unified light source.Winter when the illuminance of illuminance and daylight near the time, the water temperature in the incubator generally can not reach natural water body temperature when this illuminance; And when summer when the illuminance of illuminance and daylight near the time, the water temperature in the incubator generally can be higher than the natural water body temperature under this illumination.Its main cause is: air themperature is low than water temperature in the winter time, and the external radiations heat energy of incubator causes water temperature reduction in the case; And summer air themperature high than water temperature, cause the temperature inside the box to be higher than the natural water body temperature.Therefore the present invention is divided into artificial sun's chromatogram cold light source and separately control of electro-thermostat with illuminance and temperature control.
When process of the test is full Artificial Control, by artificial sun's chromatogram cold light source controlled light condition, by electro-thermostat control temperature; The illuminance of test and water temperature Relations Among are by the control of long term monitoring gained.
When process of the test is natural conditions, incubator is placed outside the laboratory, by natural light irradiation.Measure the control temperature of corresponding natural water body Temperature Setting electro-thermostat.
The advantage of this method:
1, by setting up the experiment station in lakeside, adopt submerged plant, bed mud and lake water in the lake, carry out culture experiment, it is convenient and swift to draw materials;
2, utilize identical parent's first filial generation to test, avoided the impact of inherent cause;
3, outdoor test can truly reasonably be simulated the actual growing environment of submerged plant;
4, full Artificial Control illumination and temperature condition of incubator is beneficial to the differentiation control to various environmental factors.
Description of drawings
Fig. 1 incubator front elevational schematic;
Fig. 2 incubator schematic top plan view.
The 1-bed mud, 2-has blind patch, the 3-electro-thermostat, the 4-seedling, 5-graduation mark, 6-lake water, the 7-water inlet pipe, 8-par groove, 9-sharp-crested weir, 10-mist air inlet pipe, the portable gas concentration lwevel instrument of 11-, 12-mist escape pipe, the 13-mist, 14-has contact with, the 15-fluorescent lamp, the 16-seal washer, the 17-apopore.
Embodiment
Below in conjunction with drawings and Examples the present invention is described in further details.
Embodiment one
(1) set up research station at Taihu Lake tribute arm of lake, select in the lake submerged plant p.malaianus as research material, fetch after laboratory cleans up, select the basically identical tender shoots of upgrowth situation, place the culture dish that fills nutrient solution, nutrient solution is mainly by NH
4NO
3And NaH
2PO
4Form, N, P concentration are respectively 2 and 0.005mg/L, and ion strength and pH are respectively 7.51 and 8.14, and nutrient solution is changed once every day, and seedling is cultivated paramount about 2cm, selects in order to testing.
(2) the thick sediments 1 of 5cm of packing in 12 incubators, be equipped with blind patch 2 at bed mud, then inject the dark lake water of 20～30cm 6, sediments 1 and lake water 6 are all taken from Taihu Lake tribute arm of lake, after water body leaves standstill 3d in the incubator, pick out seedling 4 plantations of the same size from test material for subsequent use in incubator, every case is planted 82 strains, replenish again lake water 6 to the depth of water and reach 160cm, should avoid bed mud to suspend when injecting lake water.At the incubator sidewall graduation mark 5 control bed mud and the lake water degree of depth are set.Top blind flange adopts four limit seal washers 16, and every limit is joined 2 and had contact with 14, makes incubator airtight.
(3) 12 incubators are divided into 4 groups, are followed successively by A, B, C, D, 3 every group, every day, 24h continued to pass into different CO in 4 groups of incubators
2The mist 13 of concentration, CO
2Concentration is respectively 370 μ mol/mol, 550 μ mol/mol, 700 μ mol/mol, 1000 μ mol/mol, and mist 13 is entered by entrance 10, and outlet 12 is overflowed, and incubator is placed the outdoor culture experiment that carries out of lakeside research station, and incubation time is 2 months.If carry out laboratory test, can adopt fluorescent lamp 15 and electric thermostat 3 controlled lights and temperature condition.
Duration of test slowly injects par groove 8 by water inlet pipe 7 with lake water, and sharp-crested weir 9 top height are 160cm, and when trough inner water was higher than 160cm deeply, unnecessary water overflowed automatically, makes the depth of water in the incubator maintain all the time 160cm, CO
2Concentration is by portable CO
2Concentration analyzer 11 monitorings.
(4) during off-test, adopt drainpipe 17 lake water in the incubator of draining, extract the p.malaianus blade, measure its Morphologic Parameters and amount of growth and analyze, the mean value of the absolute increment of length of blade, width and area sees Table 1; With blade at 75 ℃ of lower continuous drying 48h to constant, the record biomass, see Table 1; Blade after the oven dry is pulverized, measured its carbon, nitrogen, phosphorus and soluble sugar content, list in table 2.Can obtain CO by upper analysis
2The grow correlation of each index of change in concentration and p.malaianus is to investigate CO
2Concentration is on the impact of submerged plant growth.
Table 1 leaf growth amount and biomass
Biochemical substances and Mineral Concentrations in table 2 blade
Claims (6)
1. different gas concentration lwevels are on the test method of submerged plant growth impact, set up research station on the natural lake limit, water in the collection lake and bed mud are as test raw material, guaranteeing under the identical condition of the various growth factors of submerged plant, carry out the different gas concentration lwevel of many groups to the experimental study of submerged plant growth impact, it is characterized in that: comprise the steps
(1) sets up research station on the natural lake limit, in the lake, select a kind of submerged plant, fetch after laboratory cleans up, select the basically identical tender shoots of upgrowth situation, breeding is to the first filial generation, get with parent and the basically identical tender shoots of upgrowth situation, place the culture dish that fills nutrient solution, select in order to test;
(2) the thick sediments of 5cm of packing in 12 incubators, then inject the dark lake water of 20～30cm, sediments is all taken from the lake identical with submerged plant with lake water, after water body leaves standstill 3 days in the incubator, from test material for subsequent use, pick out seedling plantation of the same size in incubator, every case is planted 82 strains, replenishes lake water to the depth of water again and reaches 160cm, should avoid bed mud to suspend when injecting lake water;
(3) 12 incubators are divided into 4 groups, 3 every group, every day, 24h continued to pass into different CO in 4 groups of incubators
2The mist of concentration carries out culture experiment, and incubation time is 2 months; The depth of water in the duration of test, incubator all maintains 160cm, CO
2Concentration is by portable CO
2The concentration analyzer monitoring;
(4) during off-test, measure plant growth amount and morphological parameters: take out the blade of submerged plant, measure length, width and the area of blade, calculate each parameter absolute increment; With blade at 75 ℃ of lower continuous drying 48h to constant, the record biomass; Plant leaf after the oven dry is pulverized, measured biochemical substances and Mineral Concentrations in the blade.
2. test method as claimed in claim 1 is characterized in that: the culture fluid in the described step (1), and by NH
4NO
3And NaH
2PO
4Form, N, P concentration are respectively 2mg/L and 0.005mg/L, and ion strength is that 7.51, pH value is 8.14, and described nutrient solution is changed once every day.
3. test method as claimed in claim 1, it is characterized in that: the incubator in the described step (2) (3), adopting the length of side is the cube of 200cm, each face all adopts transparent organic glass to make, sealing adopts four limit packing rings to match the hook-type cover plate, and have contact with on two on every limit, and the incubator sidewall is along highly being provided with graduation mark, in relative top side wall the carbon dioxide gas mixture access way is set, at sidewall the Inlet and outlet water passage is set.
4. test method as claimed in claim 1, it is characterized in that: the incubator bed mud top in the described step (2) (3) arranges one blind patch.
5. test method as claimed in claim 1 is characterized in that: four jiaos of incubators in the described step (2) (3) arrange electro-thermostat below the water surface.
6. test method as claimed in claim 1 is characterized in that: the incubator in the described step (2) (3) is when process of the test is full Artificial Control, by artificial sun's chromatogram cold light source controlled light condition, by electro-thermostat control temperature; When process of the test is natural conditions, incubator is placed outside the laboratory, by natural light irradiation.
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| CN 201110072904 CN102172178B (en) | 2011-03-25 | 2011-03-25 | Method for testing influence of different carbon dioxide concentrations on growth of submerged plants |
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| CN103675230B (en) * | 2013-11-20 | 2015-06-03 | 中国矿业大学(北京) | Experiment device and method for simulating influence of leakage of CO2 on earth surface environment |
| CN106489581A (en) * | 2016-12-06 | 2017-03-15 | 环境保护部南京环境科学研究所 | Experimental provision and its method that a kind of water depth simulation and turbidity are affected on water plant |
| JP6642900B2 (en) * | 2017-01-23 | 2020-02-12 | ヤンマー株式会社 | Carbon dioxide fertilizer |
| CN109496588A (en) * | 2019-01-23 | 2019-03-22 | 中国科学院昆明植物研究所 | Gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation |
| CN110187052A (en) * | 2019-03-08 | 2019-08-30 | 合肥学院 | It is a kind of based on the plant-growth factor of cloud computing to the analysis method of its growth effect |
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