CN109496588A - Gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation - Google Patents
Gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation Download PDFInfo
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- CN109496588A CN109496588A CN201910064883.5A CN201910064883A CN109496588A CN 109496588 A CN109496588 A CN 109496588A CN 201910064883 A CN201910064883 A CN 201910064883A CN 109496588 A CN109496588 A CN 109496588A
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- paris polyphylla
- gas concentration
- concentration lwevel
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/02—Treatment of plants with carbon dioxide
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
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- Forests & Forestry (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Cultivation Of Plants (AREA)
Abstract
The present invention provides gas concentration lwevel and increases the method for accelerating paris polyphylla biomass accumulation, and this method includes promoting gas concentration lwevel in environment, accumulates Paris polyphylla phytomass, including paris polyphylla preculture, gas concentration lwevel handle incubation step.The present invention relates to technical field of bioengineering, are carbon dioxide (CO2) concentration increase promote paris polyphylla biomass accelerated accumulation a kind of new way.CO in environment2Concentration increases the accumulation for accelerating paris polyphylla seedling fresh weight.This plays the role of promoting production in actual production.By primary carbon source content needed for changing the paris polyphylla promoted in environment, to meet the needs of increasing production in actual production to paris polyphylla, paris polyphylla cultivating and growing efficiency is further promoted.
Description
Technical field:
The invention belongs to technical field of bioengineering, and in particular to carbon dioxide promotes the synthetic method of biomass, more
Body, it is related to gas concentration lwevel and increases a kind of method for accelerating paris polyphylla biomass accumulation.
Background technique:
Carbon dioxide (CO2) it is the main source that organic matter is formed in plant, important biochemistry is taken part in plant
Effect.CO in general atmosphere2Concentration be 350-400ppm.As the primary carbon source of plant, CO2Concentration rise after, plant
Biomass accelerates accumulation.It is mentioned in many articles, the complete stool biomass and each organ biomass of perennial plant are to CO2Concentration
Response.De Souza et al. discovery, in high CO2After concentration processing, total biomass, Leaf biomass, the stem biology of sugarcane
Amount and plant height are significantly higher than normal CO2Concentration (De Souza, Gaspar etc., 2008).It is also reported in other articles, it is white
Birch is carrying out high concentration CO2The biomass of treated plant stem and root significantly rises (Fu, Ye etc., 2010).It is tried in crop field
It tests middle discovery and applies gas CO2Fertilizer, i.e. CO in raising environment2Concentration can be such that crops significantly increase production, for example, tomato, cucumber and
Tobacco etc..But it is a small number of that the processing method, which is applied in medicinal plant,.
Paris polyphylla belongs to perennial ratio of rhizome medicinal material, raw material of the rhizome frequently as clinical application and pharmaceuticals industry.With weight
Building is that the Chinese patent drug of raw material and personal care product reach hundreds of, about 10,000,000,000 yuan of annual value of production.Its of paris polyphylla utilizes overground part
Divide and carry out photosynthesis, generate organic matter, the biomass of its aerial part can be all transferred in rhizome when annual sprout tumble starts.
It has been reported that the paris polyphylla root biomass at third year is 2.2g, it can just accelerate accumulation biology in the 4th year paris polyphylla
It measures (Zhao Tingzhou, Wang Buqiong etc., 2014).It can be seen that the paris polyphylla speed of growth is slow.
So far, it there are no in the prior art by promoting CO in paris polyphylla growing environment2Concentration, make paris polyphylla
The report of the method for biomass accumulation speed energy Rapid Accumulation.
Summary of the invention:
The purpose of the present invention is to provide one kind by promoting CO in environment2Concentration makes paris polyphylla biomass Rapid Accumulation
Method and application.
In order to realize above-mentioned purpose of the invention, the present invention provides the following technical solutions:
Gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation, and this method includes promoting titanium dioxide in environment
Concentration of carbon accumulates Paris polyphylla phytomass.
Gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation, and this method includes paris polyphylla preculture, two
It aoxidizes concentration of carbon and handles incubation step.
Gas concentration lwevel as mentioned increases the method for accelerating paris polyphylla biomass accumulation, and this method includes following steps
It is rapid:
The paris polyphylla pre-culture step is to clean annual paris polyphylla, weigh, and is transplanted into Seedling bag, normal
Culturing room's preculture 10 days under atmospheric carbon dioxide concentration 400ppm, intensity of illumination are 300 μm of ol m-2s-1, daily illumination 12h,
Cultivation temperature is 18-23 DEG C, air humidity 60%；
The gas concentration lwevel processing incubation step is that the paris polyphylla shifting after above-mentioned preculture 10 days is put into CO2It is dense
Degree is the culturing room of 800ppm, and the intensity of illumination of culturing room is 300 μm of ol m-2s-1, daily illumination 12h, cultivation temperature is
18-23 DEG C, air humidity 60%, incubation time is 30 days and 30 days or more.
Above-mentioned gas concentration lwevel, which increases, accelerates the method for paris polyphylla biomass accumulation accelerating paris polyphylla biomass product
Application in tired.
The gas concentration lwevel, which increases, accelerates the method for paris polyphylla biomass accumulation accelerating paris polyphylla biomass product
Application in tired, wherein the application is to use: annual paris polyphylla being cleaned, weighing, transplanted into Seedling bag, normal big
Culturing room's preculture 10 days under air carbon dioxide concentration 400ppm, intensity of illumination are 300 μm of ol m-2s-1, daily illumination 12h, training
Supporting temperature is 18-23 DEG C, air humidity 60%；Then the paris polyphylla shifting after above-mentioned preculture 10 days is placed on CO2Concentration is
The culturing room of 800ppm, the intensity of illumination of culturing room are 300 μm of ol m-2s-1, daily illumination 12h, cultivation temperature 18-23
DEG C, air humidity 60%, incubation time is 30 days and 30 days or more.
Gas concentration lwevel as mentioned, which increases, accelerates the method for paris polyphylla biomass accumulation accelerating paris polyphylla biomass
Application in accumulation, wherein this method randomly selects the paris polyphylla of processing and control further after processing 0,10,20,30 day
Complete stool fresh weight is weighed respectively, as a result, it was confirmed that the paris polyphylla biomass of high concentration processing is significantly higher than the paris polyphylla biology in control
Amount, paris polyphylla plant quantity of the fresh growth significantly increases when 10,20,30 days, when the 30th day, CO2Concentration is
The biomass increment range of paris polyphylla is 0.69-2.27g when 800ppm.
Compared with prior art, the excellent benefit that technical solution of the present invention has is:
CO in growing environment2After concentration increases 30 days, the significant Rapid Accumulation of Seedling Stage paris polyphylla fresh weight is promoted.
The present invention is by promoting CO in paris polyphylla growing environment2Concentration, keep the biomass accumulation speed of paris polyphylla fast
Speed accumulation, can bring income increase to production application.This processing method can be applied to more multiple medicine for this simultaneously
With in plant.If this processing, which is applied to, also the effect equally increased production on medicinal plant, that will be to medicinal plant cultivation
Progradation is played in plantation.
The present invention is by promoting CO in culture environment2Concentration promotes paris polyphylla fresh weight, thus to practical cultivating and growing mistake
The growing environment of more advantageous paris polyphylla is provided in journey.
Detailed description of the invention:
Fig. 1 difference CO2The biomass growth rate (%) of paris polyphylla under concentration.
Specific embodiment:
In order to better understand the present invention, with reference to the accompanying drawing, the present invention is further illustrated with embodiment of the present invention
Essentiality content, but the present invention is not limited with this.
Embodiment 1:
One, experimental material and method
(1) paris polyphylla preculture condition
40 plants of annual paris polyphyllas are cleaned, weighing, is transplanted into Seedling bag, in normal atmosphere gas concentration lwevel
Culturing room's preculture 10 days under (400ppm).Intensity of illumination is 300 μm of ol m-2s-1, daily illumination 12h, cultivation temperature 18-
23 DEG C, air humidity 60%.
(2) condition of culture is tested
Wherein 20 plants of paris polyphyllas placement CO will be moved after preculture 10 days2Concentration be 800ppm culturing room, remaining 20 plants
Paris polyphylla stays in CO2Concentration is the culturing room of 400ppm (CK), starts to do comparative test.Except CO2Concentration is different outer, cultivates between two
Remaining environment condition of culture of room is identical.Intensity of illumination is 300 μm of ol m-2s-1, daily illumination 12h, cultivation temperature 18-23
DEG C, air humidity 60%.
(3) paris polyphylla fresh weight weighs
After processing 0,10,20,30 day, processing and each 5 plants of weighings complete stool fresh weight of control are randomly selected, as a result retains two
Decimal point.
Two, result of study
The biomass of paris polyphylla plant is to growing environment CO2Concentration has significant response (table 1).The paris polyphylla of high concentration processing
Biomass is significantly higher than the paris polyphylla biomass in control；Compared to the biomass of paris polyphylla in control, processing group 10,20,
Paris polyphylla plant quantity of the fresh growth significantly increases when 30 days.When the 30th day, the plant biology of processing group paris polyphylla
Amount increment range is 0.69-2.27g.The biomass growth rate (Fig. 1) of two groups of different time points of comparison processing and control, discovery
In high concentration CO2Under the conditions of, the biomass growth rate of paris polyphylla is significantly raised.
2. paris polyphylla of table is in different CO2Biomass increment (g) statistics under concentration.
Numerical value is expressed as mean+SD (n=5) in table；*: indicating that same row numerical value has significant difference；*: table
Show between same row numerical value there is extremely significant difference (P < 0.05).
Finally, it should also be noted that the above enumerated are only specific embodiments of the present invention.It is clear that the invention is not restricted to
Above embodiments, acceptable there are many improve.Those skilled in the art can directly export from present disclosure
Or the improvement associated, it is considered as protection scope of the present invention.
Claims (6)
1. gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation, it is characterised in that this method includes promoting environment
Middle gas concentration lwevel accumulates Paris polyphylla phytomass.
2. gas concentration lwevel increases the method for accelerating paris polyphylla biomass accumulation, it is characterised in that this method includes that paris polyphylla is pre-
Culture, gas concentration lwevel handle incubation step.
3. gas concentration lwevel as claimed in claim 1 or 2 increases the method for accelerating paris polyphylla biomass accumulation, feature exists
In:
The paris polyphylla pre-culture step is to clean annual paris polyphylla, weigh, and is transplanted into Seedling bag, in normal atmosphere
Culturing room's preculture 10 days under gas concentration lwevel 400ppm, intensity of illumination are 300 μm of ol m-2s-1, daily illumination 12h, culture
Temperature is 18-23 DEG C, air humidity 60%；
The gas concentration lwevel processing incubation step is that the paris polyphylla shifting after above-mentioned preculture 10 days is put into CO2Concentration is
The culturing room of 800ppm, intensity of illumination are 300 μm of ol m-2s-1, daily illumination 12h, cultivation temperature is 18-23 DEG C, air humidity
It is 60%, incubation time is 30 days and 30 days or more.
The method of paris polyphylla biomass accumulation is accelerated to accelerate Yunnan weight 4. gas concentration lwevel of any of claims 1 or 2 increases
Application in building biomass accumulation.
The method of paris polyphylla biomass accumulation is accelerated to accelerate paris polyphylla raw 5. gas concentration lwevel as claimed in claim 3 increases
Application in object amount accumulation, it is characterised in that the application is to use: annual paris polyphylla is cleaned, weighing, transplanting to nursery
In bag, culturing room's preculture 10 days at normal atmosphere gas concentration lwevel 400ppm, intensity of illumination is 300 μm of ol m-2s-1,
Daily illumination 12h, cultivation temperature are 18-23 DEG C, air humidity 60%；Then the paris polyphylla after above-mentioned preculture 10 days is moved
It is put into CO2Concentration is the culturing room of 800ppm, and the intensity of illumination of culturing room is 300 μm of ol m-2s-1, daily illumination 12h, culture temperature
Degree is 18-23 DEG C, air humidity 60%, and incubation time is 30 days and 30 days or more.
The method of paris polyphylla biomass accumulation is accelerated to accelerate paris polyphylla 6. gas concentration lwevel as claimed in claim 5 increases
Application in biomass accumulation, it is characterised in that after processing 0,10,20,30 day, randomly select the paris polyphylla of processing and control
Complete stool fresh weight is weighed respectively, as a result, it was confirmed that the paris polyphylla biomass of high concentration processing is significantly higher than the paris polyphylla biology in control
Amount, paris polyphylla plant quantity of the fresh growth significantly increases when 10,20,30 days, when the 30th day, CO2Concentration is
The biomass increment range of paris polyphylla is 0.69-2.27g when 800ppm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114586609A (en) * | 2022-03-28 | 2022-06-07 | 香港科技大学 | Planting method for improving yield and effective component content of medicinal plants |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114586609A (en) * | 2022-03-28 | 2022-06-07 | 香港科技大学 | Planting method for improving yield and effective component content of medicinal plants |
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Application publication date: 20190322 |