CN109618933A - A kind of radix pseudostellariae Seed embryo culture base and its cultural method - Google Patents
A kind of radix pseudostellariae Seed embryo culture base and its cultural method Download PDFInfo
- Publication number
- CN109618933A CN109618933A CN201910062789.6A CN201910062789A CN109618933A CN 109618933 A CN109618933 A CN 109618933A CN 201910062789 A CN201910062789 A CN 201910062789A CN 109618933 A CN109618933 A CN 109618933A
- Authority
- CN
- China
- Prior art keywords
- radix pseudostellariae
- culture base
- embryo culture
- seed embryo
- pseudostellariae seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a kind of radix pseudostellariae Seed embryo culture base and its cultural methods.The radix pseudostellariae Seed embryo culture base are as follows: contain sucrose 28-32g, agar powder 7.2-7.8g, 6- benzyl aminoadenine 0.40-0.60mg, methyl α-naphthyl acetate 0.10-0.30mg and heteroauxin 0.15-0.25mg in every liter of MS culture medium；The pH value of the radix pseudostellariae Seed embryo culture base is 5.6-6.0；The cultural method of the radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；A, radix pseudostellariae seed is first sterilized with alcohol, then is sterilized with mercuric chloride, obtain A product；B, A product are selected into superclean bench complete embryo, is inoculated in radix pseudostellariae Seed embryo culture base, obtains B product；C, B product are cultivated to get radix pseudostellariae seedling.The present invention has the germination rate that can improve the emergence of radix pseudostellariae embryo, and germination rate is up to 99%, shortens seedling-growing time, and emergence is neat, and this method can quickly provide a large amount of radix pseudostellariae seedlings, promotes radix pseudostellariae artificial growth, meets the market demand.
Description
Technical field
The present invention relates to a kind of culture medium, especially a kind of radix pseudostellariae Seed embryo culture base and its cultural method.
Background technique
Radix pseudostellariae is Caryophyllaceae radix pseudostellariae category (Pseudostellaria) plant.It is traditional Chinese medicine common drug, medicine
Power is gentle, has and replenishes qi to invigorate the spleen, is promoting production of body fluid and nourishing the lung and other effects.Radix pseudostellariae is in tcm clinical practice formula, Chinese patent drug, health medicine production
Medicinal material used is all from cultivation resource.But for a long time, radix pseudostellariae carries out vegetative propagation with root tuber, causes cylinder accumulation big
Amount virus, seriously affects its yield.People are in cultivation, and also trial is bred with seed, but radix pseudostellariae seed has not
Moultinism.Under field conditions (factors), radix pseudostellariae seed sprouting needs about one year.Releasing its dormant trait using (5 DEG C) storages of low temperature also needs
30-60 days are taken, and germination rate is not high.Scholar's Ye Zuyun discovery radix pseudostellariae embryo tissue culture can grow complete seedling, but transport
It is cultivated with the culture medium of Ye Zuyun, radix pseudostellariae kind germination rate is not high.
Summary of the invention
The purpose of the present invention is to provide a kind of radix pseudostellariae Seed embryo culture base and its cultural method.The present invention has and can improve
The germination rate of radix pseudostellariae embryo emergence, germination rate are up to 99%, shorten seedling-growing time, emergence is neat, and this method can be mentioned quickly
For a large amount of radix pseudostellariae seedlings, promotes radix pseudostellariae artificial growth, meet the market demand.
The invention is realized in this way.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: every liter of MS
Contain sucrose 28-32g, agar powder 7.2-7.8g, 6- benzyl aminoadenine 0.40-0.60mg, methyl α-naphthyl acetate 0.10- in culture medium
0.30mg and heteroauxin 0.15-0.25mg.
In radix pseudostellariae Seed embryo culture base above-mentioned, the radix pseudostellariae Seed embryo culture base are as follows: contain sugarcane in every liter of MS culture medium
Sugared 29-31g, agar powder 7.4-7.6g, 6- benzyl aminoadenine 0.45-0.55mg, methyl α-naphthyl acetate 0.15-0.25mg and heteroauxin
0.18-0.23mg。
In radix pseudostellariae Seed embryo culture base above-mentioned, the radix pseudostellariae Seed embryo culture base are as follows: contain sugarcane in every liter of MS culture medium
Sugared 30g, agar powder 7.5g, 6- benzyl aminoadenine 0.50mg, methyl α-naphthyl acetate 0.20mg and heteroauxin 0.20mg.
In radix pseudostellariae Seed embryo culture base above-mentioned, the pH value of the radix pseudostellariae Seed embryo culture base is 5.6-6.0.
In the cultural method of radix pseudostellariae Seed embryo culture base above-mentioned, it is prepared according to the following steps；
A, radix pseudostellariae seed is first sterilized with alcohol, then is sterilized with mercuric chloride, obtain A product；
B, A product are selected into superclean bench complete embryo, is inoculated in radix pseudostellariae Seed embryo culture base, obtains B product；
C, B product are cultivated to get radix pseudostellariae seedling.
It is that radix pseudostellariae seed is first used into 70- in the step a in the cultural method of radix pseudostellariae Seed embryo culture base above-mentioned
75% alcohol sterilizing 25-35s, then sterilizing 15-25min is carried out with the mercuric chloride of 0.1-0.2%.
In the cultural method of radix pseudostellariae Seed embryo culture base above-mentioned, in the step c, the temperature of culture is 14-17 DEG C.
In the cultural method of radix pseudostellariae Seed embryo culture base above-mentioned, in the step c, the intensity of illumination of culture is 1800-
2200Lx, illumination 11-13h/d.
Compared with the prior art, 6- benzyl aminoadenine main function is the shape for promoting bud in radix pseudostellariae Seed embryo culture base
At evoked callus occurs；Methyl α-naphthyl acetate can promote cell division and expand, induced synthesis adventitious root；Heteroauxin induction is thin
The division of born of the same parents, the differentiation for promoting root promote growth and take root so adding heteroauxin in culture medium；MS solid medium is available
Carry out evoked callus, or is used for embryo, stem section, stem apex and Anther Culture；Sucrose except as in culture medium carbon source and the energy in addition to,
It also plays an important role to the osmotic pressure for maintaining culture medium；Agar steady chemical structure is not easy to decompose utilization by mycelia, in culture medium
In only play coagulator.
Experiments have shown that:
1, applicant is inoculated with 100 radix pseudostellariae kinds using 1-5 of the embodiment of the present invention respectively and cultivates, and observes embryo germination
Situation grows to 3cm or so in rudiment and counts its germination rate, as a result as shown in the table；
1 radix pseudostellariae Seed embryo culture base germination rate of table
| Group | Cultivate quantity | Sprouting quantity | Germination rate |
| Embodiment 1 | 100 | 99 | 99% |
| Embodiment 2 | 100 | 98 | 98% |
| Embodiment 3 | 100 | 99 | 99% |
| Embodiment 4 | 100 | 97 | 97% |
| Embodiment 5 | 100 | 98 | 98% |
And in above embodiments 1-5, after cultivating 3d, cotyledon starts to expand radix pseudostellariae kind, and plumule starts to sprout,
3cm can be grown to after 10d.
2, the culture medium that applicant changes the radix pseudostellariae Seed embryo culture base in 1-5 of the embodiment of the present invention into Ye Zuyun is (every
Rise MS culture medium in contain 6- benzyl aminoadenine 1.0mg, methyl α-naphthyl acetate 0.2mg/L) after, respectively be inoculated with 100 radix pseudostellariae kinds into
Row culture, observes embryo germination situation, grows to 3cm or so in rudiment and count its germination rate, as a result as shown in the table；
The culture medium germination rate of 2 Ye Zuyun of table
| Group | Cultivate quantity | Sprouting quantity | Germination rate |
| Embodiment 1 | 100 | 74 | 74% |
| Embodiment 2 | 100 | 75 | 75% |
| Embodiment 3 | 100 | 76 | 76% |
| Embodiment 4 | 100 | 73 | 73% |
| Embodiment 5 | 100 | 77 | 77% |
And in above embodiments 1-5, after cultivating 7d, cotyledon starts to expand radix pseudostellariae kind, and plumule starts to sprout,
3cm can be grown to after 18d.
By table 1 and table 2 as can be seen that the present invention has the germination rate that can improve the emergence of radix pseudostellariae embryo, germination rate is high
Up to 99%, shorten seedling-growing time, emergence is neat.
In conclusion the present invention has the germination rate that can improve the emergence of radix pseudostellariae embryo, germination rate is up to 99%, shortens out
The seedling time, emergence is neat, and this method can quickly provide a large amount of radix pseudostellariae seedlings, promotes radix pseudostellariae artificial growth, meets market
Demand.
Specific embodiment
Embodiment 1.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 28g, agar powder 7.2g, 6- benzyl aminoadenine 0.40mg, methyl α-naphthyl acetate 0.10mg and heteroauxin 0.15mg.
The pH value of the radix pseudostellariae Seed embryo culture base is 5.6.
The cultural method of radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；
A, radix pseudostellariae seed is first used to 70% alcohol sterilizing 25s, then carries out sterilizing 15min with 0.1% mercuric chloride, obtains A
Product；
B, A product are selected into complete embryo with pincet and scalpel in superclean bench, is inoculated in radix pseudostellariae embryo
In culture medium, B product are obtained；
C, B product to be cultivated, the temperature of culture is 14 DEG C, and the intensity of illumination of culture is 1800Lx, illumination 11h/d,
Up to radix pseudostellariae seedling.
Embodiment 2.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 29g, agar powder 7.4g, 6- benzyl aminoadenine 0.45mg, methyl α-naphthyl acetate 0.15mg and heteroauxin 0.18mg.
The pH value of the radix pseudostellariae Seed embryo culture base is 5.7.
The cultural method of radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；
A, radix pseudostellariae seed is first used to 72% alcohol sterilizing 28s, then carries out sterilizing 17min with 0.12% mercuric chloride, obtains A
Product；
B, A product are selected into complete embryo with pincet and scalpel in superclean bench, is inoculated in radix pseudostellariae embryo
In culture medium, B product are obtained；
C, B product to be cultivated, the temperature of culture is 15 DEG C, and the intensity of illumination of culture is 1900Lx, illumination 12h/d,
Up to radix pseudostellariae seedling.
Embodiment 3.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 30g, agar powder 7.4g, 6- benzyl aminoadenine 0.50mg, methyl α-naphthyl acetate 0.20mg and heteroauxin 0.20mg.
The pH value of the radix pseudostellariae Seed embryo culture base is 5.8.
The cultural method of radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；
A, radix pseudostellariae seed is first used to 73% alcohol sterilizing 30s, then carries out sterilizing 20min with 0.15% mercuric chloride, obtains A
Product；
B, A product are selected into complete embryo with pincet and scalpel in superclean bench, is inoculated in radix pseudostellariae embryo
In culture medium, B product are obtained；
C, B product to be cultivated, the temperature of culture is 15 DEG C, and the intensity of illumination of culture is 2000Lx, illumination 12h/d,
Up to radix pseudostellariae seedling.
Embodiment 4.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 31g, agar powder 7.6g, 6- benzyl aminoadenine 0.55mg, methyl α-naphthyl acetate 0.25mg and heteroauxin 0.23mg.
The pH value of the radix pseudostellariae Seed embryo culture base is 5.9.
The cultural method of radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；
A, radix pseudostellariae seed is first used to 74% alcohol sterilizing 33s, then carries out sterilizing 22min with 0.18% mercuric chloride, obtains A
Product；
B, A product are selected into complete embryo with pincet and scalpel in superclean bench, is inoculated in radix pseudostellariae embryo
In culture medium, B product are obtained；
C, B product to be cultivated, the temperature of culture is 16 DEG C, and the intensity of illumination of culture is 2100Lx, illumination 12h/d,
Up to radix pseudostellariae seedling.
Embodiment 5.A kind of radix pseudostellariae Seed embryo culture base, the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 32g, agar powder 7.8g, 6- benzyl aminoadenine 0.60mg, methyl α-naphthyl acetate 0.30mg and heteroauxin 0.25mg.
The pH value of the radix pseudostellariae Seed embryo culture base is 6.0.
The cultural method of radix pseudostellariae Seed embryo culture base, is prepared according to the following steps；
A, radix pseudostellariae seed is first used to 75% alcohol sterilizing 35s, then carries out sterilizing 25min with 0.2% mercuric chloride, obtains A
Product；
B, A product are selected into complete embryo with pincet and scalpel in superclean bench, is inoculated in radix pseudostellariae embryo
In culture medium, B product are obtained；
C, B product to be cultivated, the temperature of culture is 17 DEG C, and the intensity of illumination of culture is 2200Lx, illumination 13h/d,
Up to radix pseudostellariae seedling.
Claims (8)
1. a kind of radix pseudostellariae Seed embryo culture base, it is characterised in that: the radix pseudostellariae Seed embryo culture base are as follows: contain in every liter of MS culture medium
There are sucrose 28-32g, agar powder 7.2-7.8g, 6- benzyl aminoadenine 0.40-0.60mg, methyl α-naphthyl acetate 0.10-0.30mg and indoles
Acetic acid 0.15-0.25mg.
2. radix pseudostellariae Seed embryo culture base according to claim 1, it is characterised in that: the radix pseudostellariae Seed embryo culture base are as follows:
Contain sucrose 29-31g, agar powder 7.4-7.6g, 6- benzyl aminoadenine 0.45-0.55mg, methyl α-naphthyl acetate in every liter of MS culture medium
0.15-0.25mg and heteroauxin 0.18-0.23mg.
3. radix pseudostellariae Seed embryo culture base according to claim 1, it is characterised in that: the radix pseudostellariae Seed embryo culture base are as follows:
Contain sucrose 30g, agar powder 7.5g, 6- benzyl aminoadenine 0.50mg, methyl α-naphthyl acetate 0.20mg and indoles in every liter of MS culture medium
Acetic acid 0.20mg.
4. radix pseudostellariae Seed embryo culture base according to claim 1, it is characterised in that: the pH of the radix pseudostellariae Seed embryo culture base
Value is 5.6-6.0.
5. a kind of cultural method of the radix pseudostellariae Seed embryo culture base as described in claim 1-4 any one, it is characterised in that: press
Following step preparation；
A, radix pseudostellariae seed is first sterilized with alcohol, then is sterilized with mercuric chloride, obtain A product；
B, A product are selected into superclean bench complete embryo, is inoculated in radix pseudostellariae Seed embryo culture base, obtains B product；
C, B product are cultivated to get radix pseudostellariae seedling.
6. the cultural method of radix pseudostellariae Seed embryo culture base according to claim 5, it is characterised in that: in the step a, be
Radix pseudostellariae seed is first used to the alcohol sterilizing 25-35s of 70-75%, then carries out sterilizing 15-25min with the mercuric chloride of 0.1-0.2%.
7. the cultural method of radix pseudostellariae Seed embryo culture base according to claim 5, it is characterised in that: in the step c, training
Feeding temperature is 14-17 DEG C.
8. the cultural method of radix pseudostellariae Seed embryo culture base according to claim 5, it is characterised in that: in the step c, training
Feeding intensity of illumination is 1800-2200Lx, illumination 11-13h/d.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910062789.6A CN109618933A (en) | 2019-01-23 | 2019-01-23 | A kind of radix pseudostellariae Seed embryo culture base and its cultural method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910062789.6A CN109618933A (en) | 2019-01-23 | 2019-01-23 | A kind of radix pseudostellariae Seed embryo culture base and its cultural method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109618933A true CN109618933A (en) | 2019-04-16 |
Family
ID=66063159
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910062789.6A Pending CN109618933A (en) | 2019-01-23 | 2019-01-23 | A kind of radix pseudostellariae Seed embryo culture base and its cultural method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109618933A (en) |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142897A (en) * | 2007-11-08 | 2008-03-19 | 上海交通大学 | Radix pseudostell adventitious root inducing and tissue culturing method |
| CN101352147A (en) * | 2008-09-11 | 2009-01-28 | 中国科学院昆明植物研究所 | Method for cultivating tissue culture seedling containing Radix pseudostellariae cyclic peptides B |
| CN102630562A (en) * | 2012-03-28 | 2012-08-15 | 青阳县九华黄精产业化协会 | Tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum |
| CN103031251A (en) * | 2012-12-07 | 2013-04-10 | 上海交通大学 | Multifunctional plant excised root culture bioreactor system |
| CN105309312A (en) * | 2015-11-09 | 2016-02-10 | 广西壮族自治区药用植物园 | Petrocosmea sinensis tissue culture and rapid propagation method |
| CN106561457A (en) * | 2016-11-07 | 2017-04-19 | 宁德师范学院 | Heterophylla hybrid seed artificial cultivating and breeding method |
| CN109042332A (en) * | 2018-09-13 | 2018-12-21 | 四川七彩林业开发有限公司 | A kind of tissue culture and rapid propagation method of tripterygium wilfordii |
| CN109197594A (en) * | 2018-08-21 | 2019-01-15 | 中国农业科学院烟草研究所 | A kind of tissue culture expanding propagation method of radix pseudostellariae virus-elimination seedlings |
-
2019
- 2019-01-23 CN CN201910062789.6A patent/CN109618933A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101142897A (en) * | 2007-11-08 | 2008-03-19 | 上海交通大学 | Radix pseudostell adventitious root inducing and tissue culturing method |
| CN101352147A (en) * | 2008-09-11 | 2009-01-28 | 中国科学院昆明植物研究所 | Method for cultivating tissue culture seedling containing Radix pseudostellariae cyclic peptides B |
| CN102630562A (en) * | 2012-03-28 | 2012-08-15 | 青阳县九华黄精产业化协会 | Tissue culture and rapid propagation method of Jiuhua Polygonatum sibiricum |
| CN103031251A (en) * | 2012-12-07 | 2013-04-10 | 上海交通大学 | Multifunctional plant excised root culture bioreactor system |
| CN105309312A (en) * | 2015-11-09 | 2016-02-10 | 广西壮族自治区药用植物园 | Petrocosmea sinensis tissue culture and rapid propagation method |
| CN106561457A (en) * | 2016-11-07 | 2017-04-19 | 宁德师范学院 | Heterophylla hybrid seed artificial cultivating and breeding method |
| CN109197594A (en) * | 2018-08-21 | 2019-01-15 | 中国农业科学院烟草研究所 | A kind of tissue culture expanding propagation method of radix pseudostellariae virus-elimination seedlings |
| CN109042332A (en) * | 2018-09-13 | 2018-12-21 | 四川七彩林业开发有限公司 | A kind of tissue culture and rapid propagation method of tripterygium wilfordii |
Non-Patent Citations (7)
| Title |
|---|
| X.M MA等: "Application of artificial seeds in rapid multiplication of Pseudostellaria heterophylla", 《AFRICAN JOURNAL OF BIOTECHNOLOGY》 * |
| 叶祖云等: "太子参种子特性及种胚离体诱导培养技术研究", 《种子》 * |
| 吴朝峰: "太子参茎尖培养脱毒及增产效果的研究", 《中国优秀博硕士学位论文全文数据库（硕士） 农业科技辑》 * |
| 夏品华等: "太子参组织培养与快速繁殖研究", 《种子》 * |
| 张小玲等主编: "《种子学基础》", 31 May 2016, 中国农业大学出版社 * |
| 谢燕等: "太子参快繁技术的优化及同源四倍体的诱导与鉴定", 《植物资源与环境学报》 * |
| 邵莉楣等: "《植物生长调节剂应用手册》", 30 June 2009, 金盾出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103250560B (en) | Cultivating method of healthcare cassia seed contained coprinus comatus | |
| CN102786333B (en) | Phellinus igniarius bag cultivation medium and method for cultivating phellinus igniarius sporophore by same | |
| CN103907478B (en) | The method that flat mushroom bonsai type is cultivated and the substratum for cultivating white mushroom | |
| CN105940960B (en) | A kind of soil covering culture method of interplanting ganoderma lucidum in tea gardens | |
| CN107197691A (en) | A kind of indoor cultivation method for Ganoderma of hickory chick | |
| CN104273028B (en) | Method for rapid in-vitro propagation of Crassulaceae plant | |
| CN108887088A (en) | The formula and its method and preparation method of a kind of five kinds of culture mediums of hickory chick production of hybrid seeds cultivation | |
| CN103651071B (en) | Schizonepeta soilless cultivation method | |
| CN102884982A (en) | Hormone-free rapid propagation organic production method of anoectochilus formosanus germchit | |
| CN105409778A (en) | Adventitious bud induction method for petioles of sterile tissue culture seedlings of Lonicera hypoglauca | |
| CN104620854B (en) | Quasi wild purple lucid ganoderma cultivation technology | |
| CN105815085A (en) | Method for cultivating strong seedlings of broccoli in batches | |
| CN103283481B (en) | A kind of pixie stool is to the exhaust processes of Pinus Massoniana Young Seedlings | |
| CN106718028A (en) | A kind of golden ear high-yield seeds production cultural method | |
| CN104041408A (en) | Potting domestication method of traditional Chinese medicinal material mint | |
| CN105918133A (en) | Tissue culture and rapid propagation method of euryodendron excelsum | |
| CN107750814A (en) | A kind of breeding method of Wild ganoderma strain | |
| CN101548646B (en) | Method for rapidly propagating aralia elata through somatic embryo and secondary somatic embryogenesis | |
| CN101743846B (en) | Method for shortening culture cycle of bag-cultivation lyophyllum decastes | |
| CN106396844A (en) | Culture medium, and applications thereof in producing tremella fuciformis strains | |
| CN105993952A (en) | Rapid breeding method of Euryodendron excelsum cultivation seedlings | |
| CN103609444B (en) | Tissue culture method for hemerocallis sempervirens araki | |
| CN104813931A (en) | Tissue culture and rapid propagation method for Dendrobium officinale | |
| CN105255744B (en) | A kind of lucidum strain purification and rejuvenation cultural method and its culture medium | |
| CN103636505A (en) | Complex breeding method of high-chlorophyll multi-tiller barley |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190416 |
|
| RJ01 | Rejection of invention patent application after publication |