CN109601287A - Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room - Google Patents
Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room Download PDFInfo
- Publication number
- CN109601287A CN109601287A CN201910060700.2A CN201910060700A CN109601287A CN 109601287 A CN109601287 A CN 109601287A CN 201910060700 A CN201910060700 A CN 201910060700A CN 109601287 A CN109601287 A CN 109601287A
- Authority
- CN
- China
- Prior art keywords
- bowl
- disease
- single plant
- resistant
- wheat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Cultivation Of Plants (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses disease resistant and breeding method, including the standby alms bowl of seed selection are identified in a kind of wheat bowl transplanting seedling stage single plant room；Bowl sowing；Intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen；It is identified in seedling stage single plant room；Bowl transplanting；Conventional breeding step.It is planted using bowl, intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen, uniform condition is provided for wheat diseases, disease-resistant single plant is as selected to provide uniform selection pressure, it is not in phenomena such as onset condition is inconsistent, susceptible uneven, it is more advantageous to and selects disease-resistant single plant, the selection pressure consistency of the selection of disease resistance is easy to control, and identify that disease-resistant wheat is not limited by time and certified wheat diseases type, method is simple, be easy to learn and use, identifies that accurate, qualification cycle is short；Disease-resistant single plant is transplanted to by bowl by experimental plot after identification, test area is greatly reduced, reduces workload, ensure that survival rate, no slow seedling phenomenon.
Description
Technical field
The present invention relates to wheat breeding technical fields, and in particular to identification is anti-in a kind of wheat bowl transplanting seedling stage single plant room
Sick breeding method.
Background technique
The method that current wheat breeding for disease resistance uses is the F in field2、F3、F4... new lines do field resistance choosing
It selects, that is, selects disease-resistant single plant, need larger test area and field single plant group, the amount of labour used is larger, and field resistance
The selection pressure consistency of selection be difficult to control.
Summary of the invention
The purpose of the present invention is in view of the above shortcomings of the prior art, provide in a kind of wheat bowl transplanting seedling stage single plant room
Identify disease resistant and breeding method.
The technical scheme is that realize in the following manner:
Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room, the described method comprises the following steps:
(1), it chooses seeds for alms bowl: selecting standby wheat advantage F2、F3... or seed and the anti-disease enzyme to be identified control of seed materials
Seed；Prepare nutrition bowl, the Nutrition Soil for being mixed with muck, pathogen strain；The pathogen strain includes aecidium kind, white powder
Germ kind, banded sclerotial blight strain, head blight strain and root rot strain；
(2), bowl is sowed: seed to be identified, anti-disease enzyme control seed 1-3 are sowed in nutritive cube body respectively and are mixed with
In the Nutrition Soil of muck, 3000-4000 seeds are sowed in each combination；
(3), nutrition bowl after planting intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen: is moved into intelligent temperature
Interior, after seed sprouting to be identified wholeheartedly a leaf to tri-leaf period when start to be inoculated with pathogen；It is gently sprayed carefully using clear water after inoculation
Mist, humid control keeps the temperature 1 round the clock in 90-93%, and by the indoor temperature control moisture condensation between 9-11 DEG C of intelligent temperature round the clock；It protects
Wen Hou, by the control of intelligent temperature indoor temperature in 14-16 DEG C of mean temperature round the clock, day temperature control at 18-20 DEG C, sunshine
12-14h, humid control is cultivated under conditions of 80-85% round the clock；Winter, spring need to use fluorescent lamp, dysprosium lamp or mercury lamp supplement
Supplementary light；When being inoculated with 2 Zhou Houzhao susceptible varieties and sufficiently falling ill, incidence is investigated, documented response type checks 1 after 2-3d again
It is secondary；
(4), it is identified in seedling stage single plant room: disease resistance being identified according to the grade scale of all kinds of diseases respectively, referring to disease-resistant mirror
Surely control seed seedling removes disease plant；
(5), bowl is transplanted: disease-resistant plant being moved into experimental plot with spacing in the rows 5-10cm, line-spacing 25-30cm after identification；
(6), conventional breeding: its agronomy is recorded in the method observation for carrying out conventional field breeding to the plant for moving into experimental plot
Shape, until choosing excellent disease-resistant single plant.
Preferably, the inoculation method of inoculation pathogen includes smear inoculation, dusting inocalation method, connects by spraying in step (3)
Kind method, spore sedimentation tower inocalation method or soil inoculation method.
The invention has the following advantages that
Wheat bowl transplanting provided by the invention identifies disease resistant and breeding method in seedling stage single plant room, is planted using bowl, intelligence
Energy green house control, seedling stage single plant indoor inoculation pathogen provide uniform condition for wheat diseases, as select
It selects disease-resistant single plant and provides uniform selection pressure, be not in phenomena such as onset condition is inconsistent, susceptible uneven, more have
Conducive to disease-resistant single plant is selected, the selection pressure consistency of the selection of disease resistance is easy to control, and identifies disease-resistant wheat not
It is limited by time and certified wheat diseases type, method is simple, be easy to learn and use, be low in cost, identification is accurate, identification
Period is short；Disease-resistant single plant is transplanted to by bowl by experimental plot after identification, test area is greatly reduced, reduces workload；
Bowl transplanting simultaneously ensure that survival rate, no slow seedling phenomenon.
Specific embodiment
Below with reference to embodiment, the present invention is further illustrated.
Embodiment 1: wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room, the method includes following steps
It is rapid:
(1), it chooses seeds for alms bowl: selecting standby wheat advantage F2、F3... or seed and the anti-disease enzyme to be identified control of seed materials
Seed；Prepare nutrition bowl, the Nutrition Soil for being mixed with muck, pathogen strain；The pathogen strain includes aecidium kind, white powder
Germ kind, banded sclerotial blight strain, head blight strain and root rot strain；
(2), bowl is sowed: seed to be identified, anti-disease enzyme control seed 1-3 are sowed in nutritive cube body respectively and are mixed with
In the Nutrition Soil of muck, 3000 seeds are sowed in each combination；
(3), nutrition bowl after planting intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen: is moved into intelligent temperature
Interior, after seed sprouting to be identified wholeheartedly a leaf when start to be inoculated with pathogen；Mist is gently sprayed using clear water after inoculation, it is wet round the clock
Degree control keeps the temperature 1 round the clock in 9 DEG C of moisture condensation 90%, and by the indoor temperature control of intelligent temperature；It is after heat preservation, intelligent temperature is indoor
Temperature control is controlled in 14 DEG C of mean temperature round the clock, day temperature at 18 DEG C, sunshine 12h, round the clock item of the humid control 80%
It is cultivated under part；Winter, spring need to supplement supplementary light with fluorescent lamp, dysprosium lamp or mercury lamp；It is abundant to be inoculated with 2 Zhou Houzhao susceptible varieties
When morbidity, incidence is investigated, documented response type is checked 1 time again after 2d；
(4), it is identified in seedling stage single plant room: disease resistance being identified according to the grade scale of all kinds of diseases respectively, referring to disease-resistant mirror
Surely control seed seedling removes disease plant；It is planted using bowl, intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen is
Wheat diseases provide uniform condition, and disease-resistant single plant is as selected to provide uniform selection pressure,
Be not in phenomena such as onset condition is inconsistent, susceptible uneven, be more advantageous to and select disease-resistant single plant, the choosing of the selection of disease resistance
It selects pressure consistency to be easy to control, and identifies that disease-resistant wheat is not limited by time and certified wheat diseases type,
Its method is simple, be easy to learn and use, be low in cost, identification is accurate, qualification cycle is short；
(5), bowl is transplanted: disease-resistant plant being moved into experimental plot with spacing in the rows 5cm, line-spacing 25cm after identification；Pass through after identification
Disease-resistant single plant is transplanted to experimental plot by bowl, greatly reduces test area, reduces workload；Bowl transplanting simultaneously ensure that
Survival rate, no slow seedling phenomenon；
(6), conventional breeding: its agronomy is recorded in the method observation for carrying out conventional field breeding to the plant for moving into experimental plot
Shape, until choosing excellent disease-resistant single plant.
Preferably, the inoculation method of inoculation pathogen includes smear inoculation, dusting inocalation method, connects by spraying in step (3)
Kind method, spore sedimentation tower inocalation method or soil inoculation method.
Embodiment 2: wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room, the method includes following steps
It is rapid:
(1), it chooses seeds for alms bowl: selecting standby wheat advantage F2、F3... or seed and the anti-disease enzyme to be identified control of seed materials
Seed；Prepare nutrition bowl, the Nutrition Soil for being mixed with muck, pathogen strain；The pathogen strain includes aecidium kind, white powder
Germ kind, banded sclerotial blight strain, head blight strain and root rot strain；
(2), bowl is sowed: seed to be identified, anti-disease enzyme control seed 1-3 are sowed in nutritive cube body respectively and are mixed with
In the Nutrition Soil of muck, 4000 seeds are sowed in each combination；
(3), nutrition bowl after planting intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen: is moved into intelligent temperature
Interior starts after seed sprouting to be identified to be inoculated with pathogen when three leaves；Mist is gently sprayed using clear water after inoculation, humidity control round the clock
System keeps the temperature 1 round the clock 93%, and by the indoor temperature control moisture condensation between 11 DEG C of intelligent temperature；It, will be in intelligent greenhouse after heat preservation
Temperature control 16 DEG C of mean temperature round the clock, day temperature control at 20 DEG C, sunshine 14h, humid control is 85% round the clock
Under the conditions of cultivated；Winter, spring need to supplement supplementary light with fluorescent lamp, dysprosium lamp or mercury lamp；2 Zhou Houzhao susceptible varieties are inoculated with to fill
When distribution disease, incidence is investigated, documented response type is checked 1 time again after 3d；
(4), it is identified in seedling stage single plant room: disease resistance being identified according to the grade scale of all kinds of diseases respectively, referring to disease-resistant mirror
Surely control seed seedling removes disease plant；
(5), bowl is transplanted: disease-resistant plant being moved into experimental plot with spacing in the rows 10cm, line-spacing 30cm after identification；
(6), conventional breeding: its agronomy is recorded in the method observation for carrying out conventional field breeding to the plant for moving into experimental plot
Shape, until choosing excellent disease-resistant single plant.
Preferably, the inoculation method of inoculation pathogen includes smear inoculation, dusting inocalation method, connects by spraying in step (3)
Kind method, spore sedimentation tower inocalation method or soil inoculation method.
Above by the specific embodiment of embodiment form, the present invention is described in detail, but this should not be managed
Solution for the above-mentioned subject area of the present invention be only limitted to more than specific embodiment, it is all based on above content of the present invention it is achieved that
Technology all belongs to the scope of the present invention.
Claims (2)
1. wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room, which is characterized in that the method includes following steps
It is rapid:
(1), it chooses seeds for alms bowl: selecting standby wheat advantage F2、F3... or the seed and anti-disease enzyme to be identified of seed materials compare kind
Son；Prepare nutrition bowl, the Nutrition Soil for being mixed with muck, pathogen strain；The pathogen strain includes aecidium kind, powdery mildew
Strain, banded sclerotial blight strain, head blight strain and root rot strain；
(2), bowl is sowed: being sowed in nutritive cube body and be mixed with muck seed to be identified, anti-disease enzyme control seed 1-3 respectively
Nutrition Soil in, 3000-4000 seeds are sowed in each combination；
(3), intelligent greenhouse control, seedling stage single plant indoor inoculation pathogen: nutrition bowl after planting is moved into intelligent greenhouse,
After seed sprouting to be identified wholeheartedly a leaf to tri-leaf period when start be inoculated with pathogen；Mist, daytime are gently sprayed using clear water after inoculation
Night humid control keeps the temperature 1 round the clock in 90-93%, and by the indoor temperature control moisture condensation between 9-11 DEG C of intelligent temperature；After heat preservation,
By the indoor temperature control of intelligent temperature in 14-16 DEG C of mean temperature round the clock, day temperature control at 18-20 DEG C, sunshine 12-14h,
Humid control is cultivated under conditions of 80-85% round the clock；Winter, spring need to supplement supplement light with fluorescent lamp, dysprosium lamp or mercury lamp
According to；When being inoculated with 2 Zhou Houzhao susceptible varieties and sufficiently falling ill, incidence is investigated, documented response type is checked 1 time again after 2-3d；
(4), it is identified in seedling stage single plant room: disease resistance being identified according to the grade scale of all kinds of diseases respectively, referring to anti-disease enzyme pair
Disease plant is removed according to seed seedling；
(5), bowl is transplanted: disease-resistant plant being moved into experimental plot with spacing in the rows 5-10cm, line-spacing 25-30cm after identification；
(6), conventional breeding: its economical character is recorded in the method observation for carrying out conventional field breeding to the plant for moving into experimental plot,
Until choosing excellent disease-resistant single plant.
2. wheat bowl transplanting according to claim 1 identifies disease resistant and breeding method in seedling stage single plant room, it is characterised in that:
The inoculation method of inoculation pathogen includes smear inoculation, dusting inocalation method, spray inoculation method, spore sedimentation tower in step (3)
Inocalation method or soil inoculation method.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910060700.2A CN109601287A (en) | 2019-01-16 | 2019-01-16 | Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910060700.2A CN109601287A (en) | 2019-01-16 | 2019-01-16 | Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109601287A true CN109601287A (en) | 2019-04-12 |
Family
ID=66020398
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910060700.2A Pending CN109601287A (en) | 2019-01-16 | 2019-01-16 | Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109601287A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418382A (en) * | 2020-03-31 | 2020-07-17 | 河南省农业科学院植物保护研究所 | Method for nondestructive inoculation of alternaria alternata on sweet potato blocks |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303866A (en) * | 2014-11-18 | 2015-01-28 | 扬州大学 | Method for identifying and evaluating wheat scab expansion resistance |
| CN105647816A (en) * | 2016-01-05 | 2016-06-08 | 青岛农业大学 | Safe quick identification method of wheat scab |
| CN107815487A (en) * | 2017-12-01 | 2018-03-20 | 中国农业科学院棉花研究所 | A kind of method of indoor identification wild cotton seedling stage resistance to verticillium wilt |
| CN108849327A (en) * | 2018-07-14 | 2018-11-23 | 山东省滨州市秋田种业有限责任公司 | A kind of method of the Yellow River and Huai He River north piece wheat-resistance to scab breeding |
| CN109197423A (en) * | 2018-10-30 | 2019-01-15 | 山东省农业科学院蔬菜花卉研究所 | A kind of cuttage and seedling culture identification method of melon powdery mildew resistance |
-
2019
- 2019-01-16 CN CN201910060700.2A patent/CN109601287A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104303866A (en) * | 2014-11-18 | 2015-01-28 | 扬州大学 | Method for identifying and evaluating wheat scab expansion resistance |
| CN105647816A (en) * | 2016-01-05 | 2016-06-08 | 青岛农业大学 | Safe quick identification method of wheat scab |
| CN107815487A (en) * | 2017-12-01 | 2018-03-20 | 中国农业科学院棉花研究所 | A kind of method of indoor identification wild cotton seedling stage resistance to verticillium wilt |
| CN108849327A (en) * | 2018-07-14 | 2018-11-23 | 山东省滨州市秋田种业有限责任公司 | A kind of method of the Yellow River and Huai He River north piece wheat-resistance to scab breeding |
| CN109197423A (en) * | 2018-10-30 | 2019-01-15 | 山东省农业科学院蔬菜花卉研究所 | A kind of cuttage and seedling culture identification method of melon powdery mildew resistance |
Non-Patent Citations (1)
| Title |
|---|
| 黄虎 等: "小麦条锈病抗锈性鉴定", 《现代农业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111418382A (en) * | 2020-03-31 | 2020-07-17 | 河南省农业科学院植物保护研究所 | Method for nondestructive inoculation of alternaria alternata on sweet potato blocks |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103918464B (en) | Winter haw Malus spectabilis and the hybrid breeding method of apple | |
| CN105684738A (en) | Sunlight greenhouse tomato culture method | |
| CN103999687A (en) | Cucurbita maxima-Chinese cabbage-Wuta-tsai rotation method | |
| CN106665130B (en) | Red pear dwarfing interstock close planting cultivation technology | |
| CN105165512A (en) | Crown inclination controlling four-season cutting rapid propagation method for alnus formosana | |
| CN103444427B (en) | Method for interplanting netted melons in strawberries in greenhouse | |
| CN107509493A (en) | A kind of vinyl house cultivated strawberry technology | |
| CN101015255A (en) | Basin Primula bulleyana culture method in greenhouse | |
| CN106386036A (en) | Method for twice blooming of potted peony in one year | |
| CN108552051A (en) | A kind of method of solarium's hybrid seed production of cucumber | |
| CN109601374B (en) | Method for carrying out rice cross breeding by using plant whole growth period breeding factory | |
| CN109601287A (en) | Wheat bowl, which is transplanted, identifies disease resistant and breeding method in seedling stage single plant room | |
| CN107211717A (en) | A kind of muskmelon heliogreenhouse early spring, strawberry cultivation method autumn and winter | |
| CN106888879A (en) | A kind of mango cultural method | |
| CN104303966A (en) | Method for cultivating wild anoectochilus formosanus | |
| CN105284590B (en) | The breeding method of precocity shape sponge gourd with a tight waist | |
| CN108401783A (en) | A kind of summer mushroom vertical soil coverage cultural method | |
| CN107548858A (en) | A kind of apple nursery stock mating system | |
| CN108739327B (en) | Seed production method of capsicum annuum nuclear male sterile dual-purpose line | |
| CN106982696A (en) | A kind of cultural method of palm | |
| CN106888741A (en) | A kind of small-sized wax gourd cultivation method for premature | |
| CN106888726A (en) | A kind of small-sized pumpkin cultivation method for premature | |
| CN106888884A (en) | A kind of persimmon cultural method | |
| CN106879412A (en) | A kind of lichee cultivation method for premature | |
| CN106888886A (en) | A kind of shaddock cultivation method for premature |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190412 |
|
| RJ01 | Rejection of invention patent application after publication |