CN109589341B - 蝼蛄提取物及其制备方法 - Google Patents
蝼蛄提取物及其制备方法 Download PDFInfo
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- CN109589341B CN109589341B CN201710910270.XA CN201710910270A CN109589341B CN 109589341 B CN109589341 B CN 109589341B CN 201710910270 A CN201710910270 A CN 201710910270A CN 109589341 B CN109589341 B CN 109589341B
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- reduced pressure
- under reduced
- petroleum ether
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- mole cricket
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- 239000000284 extract Substances 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 241001243091 Gryllotalpa Species 0.000 title description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 50
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 45
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 30
- 241000241125 Gryllotalpa gryllotalpa Species 0.000 claims abstract description 28
- 239000003208 petroleum Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000011259 mixed solution Substances 0.000 claims abstract description 20
- 239000002904 solvent Substances 0.000 claims abstract description 20
- 230000005526 G1 to G0 transition Effects 0.000 claims abstract description 19
- 238000010262 high-speed countercurrent chromatography Methods 0.000 claims abstract description 14
- 239000002994 raw material Substances 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims abstract description 8
- 238000000034 method Methods 0.000 claims description 17
- 238000010992 reflux Methods 0.000 claims description 15
- 238000009835 boiling Methods 0.000 claims description 14
- 238000000605 extraction Methods 0.000 claims description 11
- 230000014759 maintenance of location Effects 0.000 claims description 9
- 238000000926 separation method Methods 0.000 claims description 9
- 230000002401 inhibitory effect Effects 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000000338 in vitro Methods 0.000 abstract description 5
- 206010006187 Breast cancer Diseases 0.000 abstract description 3
- 208000026310 Breast neoplasm Diseases 0.000 abstract description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 24
- 230000000694 effects Effects 0.000 description 10
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 10
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 9
- 238000000105 evaporative light scattering detection Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 208000003721 Triple Negative Breast Neoplasms Diseases 0.000 description 7
- 208000022679 triple-negative breast carcinoma Diseases 0.000 description 7
- 239000003480 eluent Substances 0.000 description 6
- 239000002024 ethyl acetate extract Substances 0.000 description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- IOOMXAQUNPWDLL-UHFFFAOYSA-N 2-[6-(diethylamino)-3-(diethyliminiumyl)-3h-xanthen-9-yl]-5-sulfobenzene-1-sulfonate Chemical compound C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=C(S(O)(=O)=O)C=C1S([O-])(=O)=O IOOMXAQUNPWDLL-UHFFFAOYSA-N 0.000 description 4
- 229930012538 Paclitaxel Natural products 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 229960001592 paclitaxel Drugs 0.000 description 4
- RCINICONZNJXQF-MZXODVADSA-N taxol Chemical compound O([C@@H]1[C@@]2(C[C@@H](C(C)=C(C2(C)C)[C@H](C([C@]2(C)[C@@H](O)C[C@H]3OC[C@]3([C@H]21)OC(C)=O)=O)OC(=O)C)OC(=O)[C@H](O)[C@@H](NC(=O)C=1C=CC=CC=1)C=1C=CC=CC=1)O)C(=O)C1=CC=CC=C1 RCINICONZNJXQF-MZXODVADSA-N 0.000 description 4
- 238000001514 detection method Methods 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000007983 Tris buffer Substances 0.000 description 2
- 229960000583 acetic acid Drugs 0.000 description 2
- 230000000259 anti-tumor effect Effects 0.000 description 2
- 229940034982 antineoplastic agent Drugs 0.000 description 2
- 239000002246 antineoplastic agent Substances 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000012192 staining solution Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- HZAXFHJVJLSVMW-UHFFFAOYSA-N 2-Aminoethan-1-ol Chemical compound NCCO HZAXFHJVJLSVMW-UHFFFAOYSA-N 0.000 description 1
- 238000002835 absorbance Methods 0.000 description 1
- 238000000149 argon plasma sintering Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- WTRDCBSIGOAXLO-UHFFFAOYSA-N chloroform;ethanol;hydrate Chemical compound O.CCO.ClC(Cl)Cl WTRDCBSIGOAXLO-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000001472 cytotoxic effect Effects 0.000 description 1
- GLYLMXARZJNUEY-UHFFFAOYSA-N dichloromethane;methanol;hydrate Chemical compound O.OC.ClCCl GLYLMXARZJNUEY-UHFFFAOYSA-N 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 238000001704 evaporation Methods 0.000 description 1
- 230000008020 evaporation Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012362 glacial acetic acid Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/63—Arthropods
- A61K35/64—Insects, e.g. bees, wasps or fleas
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Insects & Arthropods (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Animal Husbandry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
Claims (11)
Priority Applications (1)
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CN201710910270.XA CN109589341B (zh) | 2017-09-29 | 2017-09-29 | 蝼蛄提取物及其制备方法 |
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CN201710910270.XA CN109589341B (zh) | 2017-09-29 | 2017-09-29 | 蝼蛄提取物及其制备方法 |
Publications (2)
Publication Number | Publication Date |
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CN109589341A CN109589341A (zh) | 2019-04-09 |
CN109589341B true CN109589341B (zh) | 2022-07-12 |
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CN201710910270.XA Active CN109589341B (zh) | 2017-09-29 | 2017-09-29 | 蝼蛄提取物及其制备方法 |
Country Status (1)
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1582970A (zh) * | 2004-06-15 | 2005-02-23 | 上海澳隆科技发展有限公司 | 一种从蝼蛄中提取的抗肿瘤有效部位及制备方法 |
CN101716192A (zh) * | 2010-01-07 | 2010-06-02 | 福建农林大学 | 一种利用高速逆流色谱分离中药蝼蛄药效学物质的方法 |
-
2017
- 2017-09-29 CN CN201710910270.XA patent/CN109589341B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1582970A (zh) * | 2004-06-15 | 2005-02-23 | 上海澳隆科技发展有限公司 | 一种从蝼蛄中提取的抗肿瘤有效部位及制备方法 |
CN101716192A (zh) * | 2010-01-07 | 2010-06-02 | 福建农林大学 | 一种利用高速逆流色谱分离中药蝼蛄药效学物质的方法 |
Non-Patent Citations (3)
Title |
---|
In Vitro Cytotoxic potential on Human Cancer Cells And Chemical Constituents of Gryllotalpa orientalis;Wei et al.;《Chemistry of Natural Compounds》;20100831;第46卷(第04期);全文 * |
东方蝼蛄醇提物对3种人类肝癌细胞株的细胞毒性研究;杨勇琴等;《中国民族民间医药》;20170831;第26卷(第15期);全文 * |
抗肿瘤中药的研究与开发新进展;杨义芳等;《国际中西医肿瘤研究论坛论文专辑》;20081231;第94页第2节 * |
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CN109589341A (zh) | 2019-04-09 |
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Address after: 200040 No. 1320 West Beijing Road, Shanghai, Jingan District Patentee after: Shanghai Pharmaceutical Industry Research Institute Co.,Ltd. Patentee after: China Pharmaceutical Industry Research Institute Co.,Ltd. Address before: 200040 No. 1320 West Beijing Road, Shanghai, Jingan District Patentee before: SHANGHAI INSTITUTE OF PHARMACEUTICAL INDUSTRY Patentee before: CHINA STATE INSTITUTE OF PHARMACEUTICAL INDUSTRY |
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Effective date of registration: 20230625 Address after: No. 1, Binjiang East Road, Luolong Industrial Cluster, Yibin, Sichuan 644100 Patentee after: China Pharmaceutical Group Yibin Pharmaceutical Co.,Ltd. Address before: 200040 No. 1320 West Beijing Road, Shanghai, Jingan District Patentee before: Shanghai Pharmaceutical Industry Research Institute Co.,Ltd. Patentee before: China Pharmaceutical Industry Research Institute Co.,Ltd. |