CN109580591B - Measuring chamber and chemiluminescence detector - Google Patents
Measuring chamber and chemiluminescence detector Download PDFInfo
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- CN109580591B CN109580591B CN201710897776.1A CN201710897776A CN109580591B CN 109580591 B CN109580591 B CN 109580591B CN 201710897776 A CN201710897776 A CN 201710897776A CN 109580591 B CN109580591 B CN 109580591B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/06—Illumination; Optics
- G01N2201/064—Stray light conditioning
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Abstract
The invention relates to a measuring chamber and a chemiluminescence detector, wherein the measuring chamber comprises a bottom plate, an inner shell, an outer shell, an upper cover, a reaction cup turntable, a substrate sprayer and a photomultiplier detection assembly, an accommodating space is defined by the bottom plate, the outer shell and the upper cover, the reaction cup turntable is arranged in the accommodating space, the inner shell is arranged in the accommodating space, the reaction cup turntable is arranged between the inner shell and the outer shell along the radial direction of the reaction cup turntable, the reaction cup turntable is positioned between the upper cover and the bottom plate along the axial direction of the reaction cup turntable, a first labyrinth structure is formed at the joint of the top of the outer shell and the reaction cup turntable, and/or a second labyrinth structure is formed at the joint of the top of the inner shell and the reaction cup turntable. The measuring chamber adopts a folded surface type labyrinth structure, so that external natural light cannot reach a darkroom of the measuring chamber, and the measuring precision is improved. Meanwhile, the folded surface type labyrinth structure has a dynamic sealing effect, so that a plurality of reaction cups which are operated in parallel on each reaction cup processing station cannot be mutually interfered by light.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a chemiluminescence detector.
Background
The biochemical luminescence immunoassay method is a non-radioactive labeling immunoassay method established on the theoretical basis of the radioactive immunoassay technology and taking a labeling method optical machine as a tracing signal, and has the advantages of high sensitivity, wide restriction range, easy operation, easy realization of automation and the like. At present, with the rapid development of biomedical equipment, the realization of the full automation of biochemical luminescence detectors has met certain conditions, and biochemical luminescence detectors based on biochemical luminescence immunoassay methods have gradually become mainstream medical diagnostic equipment,
the measuring chamber is a key component influencing the accuracy and the measuring speed of the measuring result of the chemiluminescence detector, and particularly the tightness of the measuring chamber determines the accuracy and the measuring speed of the measuring result. For example, the measuring chamber disclosed in chinese patent document CN205449807U can only work in series, that is, only one cuvette can be placed in the measuring chamber and the lid can be closed to measure each time, and then the lid can be opened to take out the cuvette after the measurement is completed, and when one cuvette processing station of the measuring chamber works, other cuvette processing stations are in an idle state, so that the operations of accessing and accessing the cuvette, pumping in an excitation substrate, measuring photons, processing waste liquid, and the like cannot be processed in parallel at the same time, and the measuring speed of the instrument is severely reduced.
Disclosure of Invention
In view of the above, it is desirable to provide a measuring chamber and a chemiluminescence detector, which can improve the measurement accuracy.
The invention provides a measuring chamber which comprises a bottom plate, an inner shell, an outer shell, an upper cover, a reaction cup rotating disc, a substrate spray head and a photomultiplier detection assembly, wherein an accommodating space is defined by the bottom plate, the outer shell and the upper cover, the reaction cup rotating disc is arranged in the accommodating space, the inner shell is arranged in the accommodating space, the reaction cup rotating disc is arranged between the inner shell and the outer shell along the radial direction of the reaction cup rotating disc, the reaction cup rotating disc is positioned between the upper cover and the bottom plate along the axial direction of the reaction cup rotating disc, a first labyrinth structure is formed at the connecting part of the top of the outer shell and the reaction cup rotating disc, and/or a second labyrinth structure is formed at the connecting part of the top of the inner shell and the reaction cup rotating disc.
The invention provides a chemiluminescence detector, which comprises a measuring chamber, wherein the measuring chamber comprises: the reaction cup rotating disc is arranged between the inner shell and the outer shell along the radial direction of the reaction cup rotating disc, the reaction cup rotating disc is positioned between the upper cover and the bottom plate along the axial direction of the reaction cup rotating disc, a first labyrinth structure is formed at the joint of the top of the outer shell and the reaction cup rotating disc, and/or a second labyrinth structure is formed at the joint of the top of the inner shell and the reaction cup rotating disc.
The measuring chamber and the chemiluminescence detector adopt a folded labyrinth structure, so that external natural light cannot reach a darkroom of the measuring chamber, and the measuring precision is improved;
meanwhile, the measuring chamber and the chemiluminescence detector adopt a folded-surface labyrinth structure, and have a dynamic sealing effect, so that a plurality of reaction cups which are operated in parallel on each reaction cup processing station cannot be subjected to mutual optical interference, the measuring chamber and the reaction cup processing stations can be operated in parallel, and the measuring speed of the detector is greatly improved.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings of the embodiments can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic view of a measurement chamber according to an embodiment;
FIG. 2 is a cross-sectional view of the measurement chamber of FIG. 1;
FIG. 3 is a schematic view of the structure of the bottom plate of the measuring chamber shown in FIG. 2;
FIG. 4 is a schematic structural view of an inner housing of the measurement chamber of FIG. 2;
FIG. 5 is a schematic view of the housing of the measurement chamber of FIG. 2;
FIG. 6 is a schematic view of the upper cover of the measuring chamber shown in FIG. 2;
FIG. 7 is a schematic view of the reaction cup rotating disk of the measuring chamber of FIG. 2;
FIG. 8 is a schematic view of the reaction cup rotating disk of the measuring chamber shown in FIG. 2 in another direction.
FIG. 9 is a schematic view of an integrated structure of the bottom plate, the inner casing and the outer casing of the measuring chamber in another embodiment.
Detailed Description
In order to facilitate understanding of the present invention, the measuring chamber, the parallel working method of the measuring chamber and the multiple reaction cup processing stations, the chemiluminescence measuring method and the chemiluminescence detector will be more fully described with reference to the accompanying drawings. The preferred embodiments of the measuring chamber, the parallel working method of the measuring chamber and the multiple reaction cup processing stations, the chemiluminescence measuring method and the chemiluminescence detector are shown in the attached drawings. However, the measurement chamber, the method for parallel operation of the measurement chamber and the plurality of cuvette processing stations, the chemiluminescence assay method and the chemiluminescence detector can be realized in many different forms and are not limited to the embodiments described herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete in the measurement chamber, the method of parallel operation of the measurement chamber with multiple reaction cup processing stations, the method of chemiluminescence assay, and the chemiluminescence detector.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the measurement chamber, the method of parallel operation of the measurement chamber with multiple reaction cup processing stations, the method of chemiluminescence assay, and the chemiluminescence detector is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 and 2, a measuring chamber 1000 in one embodiment includes a bottom plate 1110, an inner housing 1120, an outer housing 1130, an upper cover 1140, a cuvette carousel 1700, a driving mechanism 1200, a first substrate ejection head 1300, a second substrate ejection head 1900, a photomultiplier tube detection assembly 1400, and a waste liquid suction needle assembly 1500. The bottom plate 1110, the outer shell 1130 and the upper cover 1140 form an accommodation space (not labeled) of the measuring chamber 1000, the reaction cup rotating disc 1700 and the inner shell 1120 are located in the accommodation space, and the bottom plate 1110, the outer shell 1130, the inner shell 1120, the upper cover 1140 and the reaction cup rotating disc 1700 form a dark room (not labeled). The measuring chamber 1000 comprises four reaction cup processing stations sealed against each other:
a first reaction cup processing station (reaction cup entry station) where reaction cups are put in or taken out from the measuring chamber 1000;
a second reaction cup processing station (a position for adding an excitation substrate I), wherein the first substrate spray nozzle 1300 is arranged at the second reaction cup processing station to add the excitation substrate I into the reaction cup;
a third reaction cup processing station (reaction cup measuring station), wherein a second substrate spray nozzle 1900 is arranged at the third reaction cup processing station to add an excitation substrate II into the reaction cup, and a photomultiplier detection assembly 1400 is arranged at the third reaction cup processing station;
a fourth reaction cup treatment station (waste liquid extraction station), and a waste liquid suction needle assembly 1500 is arranged at the fourth reaction cup treatment station to extract waste liquid of the reaction cup.
The first reaction cup processing station, the second reaction cup processing station, the third reaction cup processing station and the fourth reaction cup processing station are sequentially arranged along the rotation direction of the reaction cup rotating disc 1700 and are sealed in a light isolation mode, the first reaction cup processing station and the third reaction cup processing station are arranged diagonally, and the second reaction cup processing station and the fourth reaction cup processing station are arranged diagonally.
Referring to fig. 3, in an embodiment, the bottom plate 1110 defines a first through hole 1112, and the bottom plate 1110 sequentially forms a fourth annular boss 1116 and a fifth annular boss 1114 along the radial direction of the first through hole 1112. The fourth annular boss 1116 is formed by the outer edge of the bottom plate 1110 protruding upwards, the central axes of the fifth annular boss 1114, the fourth annular boss 1116, the first through hole 1112 and the reaction cup turntable 1700 are the same, and the inner diameter of the fifth annular boss 1114 is smaller than that of the fourth annular boss 1116. A first receiving groove 1115 is formed between the fourth annular boss 1116 and the fifth annular boss 1114, and a second receiving groove 1117 is formed between the fifth annular boss 1114 and the first through hole 1112. The bottom plate 1110 is further provided with an opening 1118, and the opening 1118 is used for installing the waste liquid sucking needle assembly 1500.
Referring to fig. 4, in an embodiment, the inner shell 1120 is cylindrical, and an annular groove 1121 is formed at an outer edge of a top portion of the inner shell 1120, so that a second annular boss 1122 is formed at the top portion of the inner shell 1120 (that is, the second annular boss 1122 is located at an end of the inner shell 1120 close to the cup carousel 1700), and the inner shell 1120 is provided with a second through hole 1124. A light source is installed on the inner wall of the inner case 1120 corresponding to the second through hole 1124. The second through hole 1124 is for passing light emitted from the light source.
Referring to fig. 5, in an embodiment, the housing 1130 is cylindrical, and a fourth annular groove 1132 is formed at an outer edge of a bottom of the housing 1130, so that a first neck portion 1131 and a first shoulder portion 1133 are formed at the bottom of the housing. The outer edge of the top of the housing 1130 is provided with a third annular groove 1134, so that a third neck 1137 and a third shoulder 1135 are formed at the top of the housing, the inner cavity of the housing 1130 is a stepped hole 1136, the stepped hole 1136 comprises a first hole 11361 and a second hole 11362, the first hole 11361 and the second hole 11362 are respectively located at the top end and the bottom end of the housing 1130, the diameter of the first hole 11361 is greater than that of the second hole 11362, and a first annular groove 11363 is formed at the joint of the first hole 11361 and the second hole 11362.
Further, in an embodiment, a third through hole 1138 is formed in a side wall of the housing 1130, and referring to fig. 1, the photomultiplier tube detecting assembly 1400 is installed on the housing 1130 corresponding to the third through hole 1138. The photomultiplier tube detector assembly 1400 detects the number of photons generated by the chemiluminescent immunoassay reaction performed in the dark room (not shown) through the third through hole 1138. The housing 1130 is further provided with a fourth through hole 1139 for mounting the optical coupler 1600. The optical coupler 1600 is used for detecting whether a reaction cup is in the second reaction cup processing station in a darkroom (not marked in the figure).
Referring to fig. 6, in an embodiment, the upper cover 1140 includes a circular cover plate 1141 and a third annular projection 1142, an outer edge of the cover plate 1141 protrudes downward to form the third annular projection 1142, the third annular projection 1142 surrounds a central axis of the cover plate 1141, the cover plate 1141 includes a first surface (i.e., a bottom surface of the cover plate) and a second surface (i.e., a top surface of the cover plate) opposite to each other, the first surface is located at the bottom, and the second surface is located at the top. The center of the first surface of the cover plate 1141 is provided with a first blind hole 1143, the center of the bottom surface of the first blind hole 1143 is sequentially and downwardly protruded to form a first round platform 1145 and a second round platform 1146, the first round platform 1145 is close to the bottom surface of the first blind hole 1143, the second round platform 1146 is far away from the bottom surface of the first blind hole 1143, the diameter of the first round platform 1145 is larger than that of the second round platform 1146, and the central axes of the first round platform 1145, the second round platform 1146, the first blind hole 1143 and the cover plate 1141 are the same axis. The first surface of the cover plate 1141 is provided with four first light-shielding component receiving grooves 1147 symmetrically distributed along the circumferential direction of the cover plate 1141, the first light-shielding component receiving grooves 1147 extend along the radial direction of the first surface and are connected between the first blind hole 1143 and the third annular boss 1142, and the four first light-shielding component receiving grooves 1147 are symmetrically distributed relative to the central axis of the third annular boss 1142, so that the cover plate 1141 is divided into four regions. Further, in one embodiment, the upper cover 1140 further defines a cup port 1144.
Referring to fig. 7 and 8, in one embodiment, the reaction cup rotating disk 1700 is disposed between the inner housing 1120 and the outer housing 1130 along a radial direction of the reaction cup rotating disk 1700, and the reaction cup rotating disk 1700 is disposed between the upper cover 1140 and the bottom plate 1110 along an axial direction of the reaction cup rotating disk 1700. Reaction cup carousel 1700 includes a top plate 1720, a peripheral wall 1740, and a shaft 1760, where the top plate 1720 is in the shape of a circular plate and includes a first surface and a second surface that are oppositely disposed, the first surface is located at the bottom, the second surface is located at the top, and the second surface is in clearance fit with the upper cover 1140.
Further, in one embodiment, peripheral wall 1740 is generally cylindrical and extends vertically downward from the first surface of top plate 1720, and peripheral wall 1740 surrounds the interior cavity forming cup carousel 1700. The diameter of the top plate 1720 is larger than the outer diameter of the peripheral wall 1740 so that the top plate 1720 projects radially outward relative to the peripheral wall 1740 to form a first annular ledge 1730. The peripheral wall 1740 includes a first end close to the first surface of the top plate 1720 and a second end far from the first surface of the top plate 1720, and a fifth annular recess 1790 is formed inside the second end (the bottom surface of the peripheral wall) of the peripheral wall 1740, and the fifth annular recess 1790 surrounds the rotation central axis of the reaction cup rotating disk.
Further, in one embodiment, shaft 1760 extends vertically downward from the center of the first surface of top plate 1720. Pivot 1760 includes a first end proximate to the first surface of top plate 1720 and a second end distal to the first surface of top plate 1720. A notch 1762 is formed at the second end of the rotating shaft 1760, the notch 1762 penetrates through the second end of the rotating shaft 1760 along the horizontal direction and forms two oppositely arranged bumps 1764, the two bumps 1764 are respectively symmetrically arranged at two sides of the notch 1762, a second blind hole 1766 is formed in the top surface of the notch 1762, and the central axis of the second blind hole 1766 is the same as the central axis of the rotating shaft 1760.
Further, in one embodiment, the first surface of top plate 1720 extends downward along the inner edge of perimeter wall 1740 to form a first ring platform 1780, wherein the height of first ring platform 1780 is less than the height of perimeter wall 1740, and first ring platform 1780 encircles the central axis of reaction cup carousel 1700. The first ring table 1780 includes a first end and a second end disposed opposite to each other, the first end is close to the first surface of the top plate 1720, the second end is far away from the first surface of the top plate 1720, the second end of the first ring table 1780 (the bottom surface of the first ring table) is provided with a second annular groove 1770 along the circumference of the first ring table 1780, so as to divide the first ring table 1780 into a first portion 1782 and a second portion 1784 which are spaced apart from each other, the first portion 1782 and the second portion 1784 are respectively annular and respectively surround the rotation central axis of the reaction cup rotating disk 1700, and the second portion 1784 is located at the radial outer side of the first portion 1782.
Further, in an embodiment, a stepped hole 1722 is formed in the center of the second surface of the top plate 1720, the stepped hole 1722 includes a large hole and a small hole, the large hole is located above the small hole, and a step surface is formed at the joint of the large hole and the small hole, and the step surface is annular and surrounds the rotation central axis of the reaction cup rotating disk 1700. The second surface is protruded upwards at the periphery of the large hole to form a second ring stage 1724, and the second ring stage 1724 is in a ring shape and surrounds the central axis of rotation of the reaction cup turntable 1700. The second surface is provided with four reaction cup accommodating cavities 1726 for accommodating reaction cups, the outer surface of the peripheral wall 1740 of the reaction cup turntable 1700 is provided with four measuring windows 1742, the four measuring windows 1742 are in one-to-one correspondence and communication with the four reaction cup accommodating cavities 1726, the inner surface of the peripheral wall 1740 of the reaction cup turntable 1700 is provided with light holes 1744, the light holes 1744 are communicated with the measuring windows 1742, and the light holes 1744 are communicated with the inner cavity of the reaction cup turntable 1700 and the measuring windows 1742. When the head of the reaction cup is hung in the reaction cup accommodating cavity 1726, the body of the reaction cup extends into the measuring window 1742, so that the body of the reaction cup can be observed. The second surface is provided with four second light-shielding assembly accommodating grooves 1728, the second light-shielding assembly accommodating grooves 1728 extend along the radial direction of the top plate 1720 and are connected between the large hole and the outer edge of the top plate 1720, the four second light-shielding assembly accommodating grooves 1728 are symmetrically distributed relative to the rotation central axis of the reaction cup rotating disc 1700, so that the top plate 1720 is divided into four reaction cup accommodating areas, the reaction cup rotating disc 1700 is correspondingly divided into four reaction cup accommodating areas, and each reaction cup accommodating area is correspondingly provided with a reaction cup accommodating cavity 1726. The number of the four second light-shielding assembly accommodating grooves 1728, the number of the four first light-shielding assembly accommodating grooves 1147, the number of the four light-shielding assemblies, the number of the four reaction cup processing stations and the number of the four reaction cup accommodating areas are in one-to-one correspondence. When the cuvette turntable 1700 rotates to rotate the four cuvette receiving areas to the corresponding cuvette processing stations, the four second light-shielding assembly receiving slots 1728 correspond to the four first light-shielding assembly receiving slots 1147 one by one, and each first light-shielding assembly receiving slot 1147 and the corresponding second light-shielding assembly receiving slot 1728 together form a space for receiving a light-shielding assembly.
With reference to fig. 2-4, in an embodiment, the inner housing 1120 is disposed in a receiving space (not labeled) of the measuring chamber, the inner housing 1120 is fixedly installed in the second receiving groove 1117 of the bottom plate 1110, and an outer wall of the inner housing 1120 abuts against a side of the fifth annular boss 1114 of the bottom plate 1110 near the first through hole 1112. The inner housing 1120 is positioned within the inner cavity of the cup carousel 1700, and the inner housing 1120 is axially positioned between the first surface of the top plate 1720 and the bottom plate 1110 of the cup carousel 1700.
Referring to fig. 8, the second annular boss 1122 is located at one end of the inner housing 1120 close to the reaction cup rotating disk 1700, the first surface of the top plate of the reaction cup rotating disk 1700 is protruded to form a first annular table 1780, the first annular table 1780 is circumferentially provided with a second annular groove 1770, the second annular boss 1122 of the inner housing 1120 is matched with the second annular groove 1770 of the reaction cup rotating disk 1700, the second annular boss 1122 is embedded into the second annular groove 1770 to form a second labyrinth structure, and when the reaction cup rotating disk rotates, the second annular boss 1122 and the second annular groove 1770 can slide relatively. The inner housing 1120 is installed in the inner cavity of the cup carousel 1700, and a gap exists between the outer wall of the inner housing 1120 and the inner wall of the peripheral wall 1740 of the cup carousel 1700, which can satisfy the requirement for the rotation of the cup carousel 1700 in a dark room (not shown). In other embodiments, the second annular boss 1122 may be an annular groove, and the second annular groove 1770 that mates with the second annular boss 1122 may be an annular boss, as long as the second annular boss 1122 and the second annular groove 1770 may form a labyrinth surface.
Referring to fig. 3 and 5, in an embodiment, the housing 1130 is fixedly mounted on the first receiving groove 1115 of the bottom plate 1110, the fourth annular groove 1132 of the housing 1130 is located at an end of the housing 1130 close to the bottom plate 1110, the fourth annular groove 1132 is matched with the fourth annular protrusion 1116 of the bottom plate 1110, the first neck portion 1131 of the housing 1130 abuts against a side surface of the fourth annular protrusion 1116 close to the first receiving groove 1115, and the first shoulder portion 1133 is overlapped on a top surface of the fourth annular protrusion 1116 to form a fourth labyrinth structure. In other embodiments, the fourth annular projection 1116 may be an annular groove, and the fourth annular groove 1132 matched with the fourth annular projection 1116 may be an annular projection, as long as the fourth annular projection 1116 and the fourth annular groove 1132 may form a labyrinth surface. Further, the third through hole 1138 of the outer shell 1130 corresponds to the second through hole 1124 of the inner shell 1120, the central axes of the third through hole 1138 and the second through hole 1124 are the same, and when the reaction cup turntable 1700 conveys the reaction cup to the third reaction cup processing station, the third through hole 1138 and the second through hole 1124 are communicated with the light hole 1744 and the measuring window 1742 of the reaction cup turntable 1700.
Referring to fig. 5 and 6, in an embodiment, the upper cover 1140 is fixedly mounted on the housing 1130, a side surface of the third annular projection 1142 close to the central axis of the upper cover 1140 abuts against the third neck 1137 of the housing 1130, and a bottom surface of the third annular projection 1142 is disposed on the third shoulder 1135, so that the third annular projection 1142 is engaged with the third annular groove 1134 to form a third labyrinth structure. That is, the outer edge of the cover plate 1141 protrudes to form the third annular boss 1142, the third annular groove 1134 is located at one end of the housing 1130 close to the upper cover 1140, and the third annular boss 1142 is in contact with the third annular groove 1134 to form a third labyrinth structure. In other embodiments, third annular land 1142 may be an annular groove and third annular groove 1134 that mates with third annular land 1142 may be an annular land, as long as third annular land 1142 and third annular groove 1134 may form a labyrinth surface.
Referring to fig. 2, 5 and 7, in an embodiment, the first annular protrusion 1730 of the reaction cup rotating disk 1700 is engaged with the first annular groove 11363 of the housing 1130 to form a first labyrinth structure, a gap exists between a side surface of the first annular protrusion 1730 and a wall of the first hole 11361, the gap can satisfy that the reaction cup rotating disk 1700 rotates in a receiving space (not labeled in the figures) of the measuring chamber, a bottom surface of the first annular protrusion 1730 abuts against a bottom surface of the first annular groove 11363, and when the reaction cup rotating disk 1700 rotates, the first annular protrusion 1730 and the first annular groove 11363 can slide relatively. That is, the top plate 1720 of the reaction cup rotating disk 1700 protrudes radially outward to form the first annular protrusion 1730, the junction of the first hole and the second hole of the housing 1130 forms the first annular groove 11363, and the first annular protrusion 1730 is embedded in the first annular groove 11363 and can slide relatively, thereby forming the first labyrinth structure. In other embodiments, the first annular protrusion 1730 may be an annular groove, and the first annular groove 11363 that mates with the first annular protrusion 1730 may be an annular protrusion, as long as the first annular protrusion 1730 and the first annular groove 11363 may form a labyrinth surface. The peripheral wall 1740 of the cuvette carousel 1700 is disposed in a space defined by the bottom plate 1110, the inner shell 1120, and the outer shell 1130, and a certain gap is formed between the second end of the peripheral wall 1740 and the bottom plate 1110, and the gap can satisfy the requirement that the cuvette carousel 1700 rotates in the accommodating space (not labeled in the figures) of the measurement chamber.
Referring to fig. 4 and 8, in an embodiment, a fifth annular recess 1790 on the peripheral wall 1740 cooperates with the fifth annular boss 1114 on the bottom plate 1110 to form a fifth labyrinth structure, and the fifth annular recess 1790 and the fifth annular boss 1114 can slide relative to each other when the reaction cup rotating disk 1700 rotates. In other embodiments, the fifth annular boss 1114 may be an annular groove, and the fifth annular groove 1790 that mates with the fifth annular boss 1114 may be an annular boss, as long as the fifth annular boss 1114 and the fifth annular groove 1790 may form a labyrinth fold.
With reference to fig. 2, fig. 6 and fig. 7, in an embodiment, the second ring platform 1724 of the reaction cup rotating disk 1700 is sleeved in the first blind hole of the upper cover 1140, a side surface of the second ring platform 1724 away from the central axis of the reaction cup rotating disk 1700 abuts against a hole wall of the first blind hole 1143, and a top surface of the second ring platform 1724 abuts against a bottom surface of the first blind hole 1143. The stepped hole 1722 is used for mounting the bearing 1800, and the bearing 1800 is arranged in the large hole of the stepped hole 1722 and is received on the stepped surface formed by the large hole and the small hole. The second round platform 1146 of the upper cover 1140 extends into the stepped hole 1722 and is sleeved in the bearing 1800, and a limit step is formed at the joint of the first round platform 1145 and the second round platform 1146 of the upper cover 1140 and used for limiting the bearing 1800. That is, the bearing 1800 is axially restrained between the restraining step and the step face.
Further, in an embodiment, the light-blocking assembly (omitted in the drawings) includes an elastic member and a light-blocking plate. The elastic element is fixedly connected to the second light-shielding assembly accommodating groove 1728 through a screw, and the light-shielding plate is placed on the elastic element. When the cuvette turntable 1700 rotates to the position of the first light-shielding component accommodating groove 1147 and the position of the second light-shielding component accommodating groove 1728, the elastic element is in a spring state, the elastic element abuts the light-shielding plate against the first light-shielding component accommodating groove 1147, and the light-shielding component (omitted in the figure) is positioned in the light-shielding component accommodating cavity formed by the first light-shielding component accommodating groove 1147 and the corresponding second light-shielding component accommodating groove 1728, so that the upper cover 1140 and the cuvette turntable 1700 are sealed, and when the cuvette on the cuvette turntable 1700 is positioned at the cuvette processing station, the cuvette can be processed correspondingly. When the cuvette turntable 1700 rotates to a position where the second light-shielding assembly receiving groove 1728 is staggered from the first light-shielding assembly receiving groove 1147, the elastic element is in a compressed state, and the upper cover 1140 presses the light-shielding plate (not shown) into the second light-shielding assembly receiving groove 1728, so that the upper cover 1140 and the cuvette turntable 1700 can rotate relatively.
Referring to fig. 2, in an embodiment, the driving mechanism 1200 is fixedly installed on the base plate 1110 through a first through hole of the base plate 1110, and the driving mechanism 1200 includes a connection block 1220, a rotation platform 1240, and a motor 1260. The connection block 1220 and the rotation platform 1240 are fixedly connected, for example, by screws. The motor 1260 is fixedly connected to the rotating platform 1240, and the motor 1260 drives the rotating platform 1240 to rotate. The connecting block 1220 is engaged with the notch 1762 of the cuvette turntable 1700. When the rotary platform 1240 rotates, the connecting block 1220 fixedly connected with the rotary platform 1240 is driven to move, so that the reaction cup turntable 1700 connected with the connecting block 1220 is driven to move, and the driving mechanism 1200 drives the reaction cup turntable 1700 to rotate in the dark room.
Specifically, in one embodiment, the photomultiplier tube assembly 1400 is mounted on the housing 1130 and disposed at the third cuvette processing station, and is disposed opposite to the cuvette port 1144 for detecting the number of photons generated by the chemiluminescence immune reaction performed in the darkroom (not shown). Two substrate sprayers are arranged on the upper cover 1140 and used for adding excitation substrates into reaction cups to be tested, the first substrate sprayer 1300 is arranged on the upper cover 1140 corresponding to the optical coupler 1600 and positioned at a second reaction cup processing station, the second substrate sprayer 1900 is arranged on the upper cover 1140 corresponding to the photomultiplier detection assembly 1400 and positioned at a third reaction cup processing station, and the first substrate sprayer 1300 and the second substrate sprayer 1900 are arranged on the upper cover 1140 in a right angle. The measuring chamber 1000 may further include a waste liquid sucking needle assembly 1500 and an optical coupler 1600, wherein the waste liquid sucking needle assembly 1500 is mounted on the bottom plate 1110 and located at the fourth reaction cup processing station, and is used for sucking away waste liquid in the detected reaction cup. The optical coupler 1600 is installed on a housing 1300 of a darkroom (not marked in the figure) and is used for detecting whether a reaction cup exists in a second reaction cup processing station in the darkroom.
Further, the measuring chamber of the present invention further includes a grounding member (not shown) made of a metal material, the grounding member is installed in the second receiving groove 1117 of the bottom plate 1110 and is located in the inner cavity of the cuvette carousel 1700, the top of the grounding member is spaced from the upper cover 1140, a resilient tab capable of being elastically bent downward is disposed on the top of the grounding member, and the resilient tab is pre-pressed between the top plate 1720 of the cuvette carousel 1700 and the grounding member. Static electricity is generated during the rotation of the cuvette carousel 1700, and the grounding assembly is used to discharge the static electricity, thereby preventing the influence on the operation of the measurement chamber 1000.
In another embodiment, as shown in FIG. 9, the bottom plate 1110, the outer shell 1130, and the inner shell 1120 of the measurement chamber 1000 are integrally formed. The upper cover 1140 is fixed on the outer shell 1130, a first labyrinth structure is formed at the joint of the top of the outer shell 1130 and the reaction cup turntable 1700, and a second labyrinth structure is formed at the joint of the top of the inner shell 1120 and the reaction cup turntable 1700. Preferably, the junction of the housing 1130 and the upper cover 1140 forms a third labyrinth structure. The measurement chamber, the first labyrinth structure, the second labyrinth structure, and the third labyrinth structure are as described above and will not be described herein.
Specifically, in one embodiment, the method for parallel operation of multiple reaction cup processing stations in the measuring chamber 1000 comprises the following steps:
1) placing the first reaction cup containing the sample to be tested into the first reaction cup accommodating cavity 1726 of the reaction cup turntable 1700 at the first reaction cup processing station through the reaction cup inlet/outlet 1144 of the upper cover 1140;
2) the driving mechanism 1200 drives the reaction cup turntable 1700 to rotate by 90 degrees, the first reaction cup accommodating cavity 1726 moves to the second reaction cup processing station, the second reaction cup accommodating cavity 1726 moves to the first reaction cup processing station, and then the following steps are synchronously executed:
(1) the optical coupler 1600 detects that a reaction cup is arranged on the reaction cup rotating disc 1700, and the first substrate spray nozzle 1300 adds an excitation substrate I into the first reaction cup at the second reaction cup processing station
(2) Placing a second reaction cup into the second reaction cup accommodating cavity 1726 at the first reaction cup processing station;
3) the driving mechanism 1200 drives the reaction cup turntable 1700 to rotate by 90 degrees, the first reaction cup accommodating cavity 1726 moves to the third reaction cup processing station, the second reaction cup accommodating cavity 1726 moves to the second reaction cup processing station, the third reaction cup accommodating cavity 1726 moves to the first reaction cup processing station, and then the following steps are synchronously executed:
(1) the second substrate sprayer 1900 adds an excitation substrate II into the first reaction cup, under the action of the excitation substrate II, the specific substance in the sample to be detected emits light, and the photomultiplier detection assembly 1400 receives and records the photon quantity for detection;
(2) the first substrate showerhead 1300 adds the excitation substrate i to the second reaction cup;
(3) placing a third reaction cup into the third reaction cup accommodating cavity 1726 at the first reaction cup processing station;
4) the driving mechanism 1200 drives the reaction cup turntable 1700 to rotate by 90 °, the first reaction cup accommodating cavity 1726 moves to the fourth reaction cup processing station, the second reaction cup accommodating cavity 1726 moves to the third reaction cup processing station, the third reaction cup accommodating cavity 1726 moves to the second reaction cup processing station, the fourth reaction cup accommodating cavity 1726 moves to the first reaction cup processing station, and then the following steps are synchronously executed:
(1) the waste liquid sucking needle assembly 1500 sucks waste liquid in the first reaction cup after detection;
(2) the second substrate sprayer 1900 adds the excitation substrate II into the second reaction cup, under the action of the excitation substrate II, the specific substance in the sample to be detected emits light, and the photomultiplier detection assembly 1400 receives and records the photon quantity for detection;
(3) the first substrate sprayer 1300 adds the excitation substrate I to the third reaction cup;
(4) placing a fourth reaction cup into the fourth reaction cup accommodating cavity 1726 at the first reaction cup processing station;
5) the driving mechanism 1200 drives the reaction cup turntable 1700 to rotate by 90 degrees, the first reaction cup accommodating cavity 1726 moves to the first reaction cup processing station, the second reaction cup accommodating cavity 1726 moves to the fourth reaction cup processing station, the third reaction cup accommodating cavity 1726 moves to the third reaction cup processing station, the fourth reaction cup accommodating cavity 1726 moves to the second reaction cup processing station, and then the following steps are synchronously executed:
(1) taking out the first reaction cup from the first reaction cup processing station and putting the first reaction cup into a fifth reaction cup;
(2) the waste liquid sucking needle assembly 1500 sucks waste liquid in the second reaction cup;
(3) the second substrate sprayer 1900 adds the excitation substrate II into the third reaction cup, under the action of the excitation substrate II, the specific substance in the sample to be detected emits light, and the photomultiplier detection assembly 1400 receives and records the photon quantity for detection;
(4) the first substrate showerhead 1300 adds the excitation substrate i to the fourth reaction cup.
Specifically, in another embodiment, the method for parallel operation of multiple reaction cup processing stations in the measuring chamber 1000 comprises the following steps:
s1, placing the first reaction cup filled with the sample to be measured into the measuring chamber 1000 at the reaction cup entering station;
s2, at the cuvette measuring station, the photomultiplier tube detector assembly 1400 measures the number of photons in the second cuvette.
Preferably, the method further comprises the following steps executed synchronously: at the waste liquid pumping station, the waste liquid sucking needle assembly 1500 pumps away the waste liquid of the third reaction cup after the photon number measurement is completed.
Preferably, the method further comprises the following steps executed synchronously: at the add excitation substrate I station, the first substrate showerhead 1300 adds the excitation substrate I to a fourth reaction cup where the number of photons is to be measured.
Preferably, in the step S2, the method further includes: second substrate showerhead 1900 initially adds excitation substrate II to the second reaction cup.
Preferably, in the step S1, the method further includes: the fifth cuvette from which the waste liquid has been drawn out is taken out of the measuring chamber 1000 at the cuvette entry station.
Specifically, in one embodiment, the chemiluminescence assay method of the measurement chamber 1000 comprises the following steps:
1) placing a first reaction cup filled with a sample to be detected into a first reaction cup accommodating cavity of the reaction cup turntable 1700 on a first reaction cup processing station;
2) the reaction cup rotating disc 1700 rotates to move the first reaction cup accommodating cavity to a second reaction cup processing station, and the first substrate spray nozzle 1300 adds an excitation substrate I to the first reaction cup at the second reaction cup processing station;
3) the reaction cup turntable 1700 rotates to move the first reaction cup accommodating cavity to a third reaction cup processing station, the second substrate sprayer 1900 adds an excitation substrate II to the first reaction cup, a specific substance in a sample to be detected emits light under the action of the excitation substrate II, and the photomultiplier detection assembly 1400 receives and records the quantity of photons for detection;
4) the reaction cup rotating disc 1700 rotates to move the first reaction cup accommodating cavity to a fourth reaction cup processing station, and the waste liquid sucking needle assembly 1500 sucks waste liquid of the first reaction cup;
5) the reaction cup rotating disc 1700 rotates to move the first reaction cup accommodating cavity to a first reaction cup processing station, and the first reaction cup is taken out and put into a new reaction cup at the first reaction cup processing station.
The present invention also provides a chemiluminescence detector in one embodiment, which includes the measurement chamber 1000 shown in fig. 1 to 8.
The measuring chamber 1000 of the present invention forms a plurality of the above-described folded labyrinth structures, for example: when external natural light irradiates the measuring chamber 1000 and is transmitted to a dark room (not marked in the figure), the reduction of the natural light intensity is approximately eliminated through the reflection of a plurality of folding surfaces, so that the influence of the natural light on the detection is eliminated, and the accuracy of the detection result is improved.
In addition, the folded surface type labyrinth structure has a dynamic sealing effect, and solves the problem of residual luminous interference in the simultaneous parallel processing of multiple reaction cups in the measuring chamber 1000 in which the multiple reaction cups are processed in parallel at multiple reaction cup processing stations. When the driving mechanism 1200 drives the reaction cup rotating disc 1700 to rotate in the darkroom (not labeled in the figure), the reaction cups in the plurality of reaction cup accommodating cavities 1726 move in the darkroom (not labeled in the figure) along with the reaction cup rotating disc 1700 at the same time, and after the reaction cups are conveyed to the corresponding reaction cup processing stations, the reaction cups conveyed to the corresponding reaction cup processing stations can be processed in parallel at the same time by a plurality of different reaction cup processing stations, so that the measurement chambers realize the simultaneous work of the inlet and outlet of the reaction cups, the addition of an excitation substrate, the photon measurement and the waste liquid treatment at different reaction cup processing stations, and the measurement speed of the instrument is greatly improved.
Meanwhile, the plurality of light-blocking assemblies are arranged between the reaction cup turntable 1700 and the upper cover 1140, and the plurality of light-blocking assemblies can rotate together with the reaction cup turntable 1700 relative to the upper cover 1140 to divide the reaction cup turntable 1700 into a plurality of reaction cup accommodating areas which are sealed in a light-blocking manner, so that the light isolation effect among the plurality of reaction cup accommodating areas is good, and therefore, in the parallel work engineering of a plurality of reaction cup processing stations in a measuring chamber, the plurality of reaction cups which operate in parallel on each reaction cup processing station cannot interfere with each other, and particularly, the reaction cup of the third reaction cup processing station (reaction cup measuring station) cannot be interfered by light generated by the reaction cups of other reaction cup processing stations in the measuring process. The method not only improves the accuracy of detection results, but also solves the problem of residual luminous interference in the simultaneous parallel processing of multiple reaction cups in the same measuring chamber, so that the measuring chamber realizes the simultaneous work of the inlet and outlet of the reaction cups, the addition of an excited substrate, photon measurement and waste liquid treatment in different reaction cup processing stations, and the measuring speed of the instrument is greatly improved.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (17)
1. A measuring chamber comprises a bottom plate, an inner shell, an outer shell, an upper cover, a reaction cup turntable, a substrate spray head and a photomultiplier detection assembly, wherein an accommodating space is defined by the bottom plate, the outer shell and the upper cover;
the first labyrinth seal structure comprises a first annular boss and a first annular groove, the shell is cylindrical, a top plate of the reaction cup turntable extends radially and outwards to form the first annular boss, the inner cavity of the shell is a stepped hole, the stepped hole of the shell comprises a first hole and a second hole, the joint of the first hole and the second hole forms the first annular groove, and the first annular boss is embedded into the first annular groove and can slide relatively; the second labyrinth seal structure includes a second annular boss and a second annular groove.
2. The measurement chamber of claim 1, wherein the cuvette carousel forms one of the second annular boss and the second annular groove, and the top of the inner housing forms the other of the second annular boss and the second annular groove.
3. The measurement chamber according to claim 2, wherein the inner shell is cylindrical, the reaction cup rotating disc comprises a disc-shaped top plate and a peripheral wall, the top plate comprises a first surface and a second surface which are oppositely arranged, the peripheral wall is formed by vertically extending downwards the outer edge of the top plate, the peripheral wall surrounds the central axis of the reaction cup turntable to form an inner cavity of the reaction cup turntable, the inner shell is positioned in the inner cavity of the reaction cup rotating disc, the inner shell is positioned between the first surface of the top plate and the bottom plate of the reaction cup rotating disc along the axial direction, the second annular boss is positioned at one end of the inner shell close to the reaction cup turntable, the first surface of the top plate of the reaction cup turntable is convexly arranged to form a first annular table, the first annular table is provided with a second annular groove along the circumferential direction, and the second annular boss is embedded into the second annular groove and can slide relatively.
4. The measurement chamber of claim 1, wherein the upper cover is secured to the housing, a junction of the housing and the upper cover forming a third seal structure, the third seal structure including a third annular boss and a third annular groove, the upper cover forming one of the third annular boss and the third annular groove, the housing forming the other of the third annular boss and the third annular groove.
5. The measuring chamber as claimed in claim 4, wherein the housing is cylindrical, the upper cover includes a circular cover plate and a third annular projection, the outer edge of the cover plate protrudes to form the third annular projection, the third annular groove is located at one end of the housing close to the upper cover, and the third annular projection is in abutting engagement with the third annular groove.
6. The measurement chamber of claim 1, wherein a junction of the bottom plate and the housing forms a fourth seal structure, the fourth seal structure including a fourth annular boss and a fourth annular recess, the bottom plate forming one of the fourth annular boss and the fourth annular recess, the housing forming the other of the fourth annular boss and the fourth annular recess.
7. The measuring chamber according to claim 6, wherein the housing is cylindrical, the outer edge of the bottom plate protrudes to form the fourth annular boss, the fourth annular groove is located at one end of the housing close to the bottom plate, and the fourth annular boss is in fit abutment with the fourth annular groove.
8. The measurement chamber of claim 1, wherein a junction of the bottom plate and the reaction cup turntable forms a fifth sealing structure, the fifth sealing structure comprising a fifth annular boss and a fifth annular groove, the bottom plate forming one of the fifth annular boss and the fifth annular groove, the reaction cup turntable forming the other of the fifth annular boss and the fifth annular groove.
9. The measuring chamber as claimed in claim 8, wherein the fifth annular boss is located on the bottom plate, the outer edge of the bottom plate protrudes to form a fourth annular boss, the inner diameter of the fifth annular boss is smaller than that of the fourth annular boss, the central axis of the fifth annular boss is the same as that of the fourth annular boss, the fifth annular groove is located at one end of the reaction cup rotating disc close to the bottom plate, and the fifth annular boss is embedded in the fifth annular groove and can slide relatively.
10. The measuring chamber of claim 9, wherein the housing is cylindrical, a fourth sealing structure is formed at a joint of the bottom plate and the housing, the fourth sealing structure includes a fourth annular boss and a fourth annular groove, the fourth annular groove is located at one end of the housing close to the bottom plate, and the fourth annular boss and the fourth annular groove are in matched abutment.
11. The measuring chamber according to claim 1, further comprising a driving mechanism, wherein the outer casing, the inner casing and the bottom plate are integrally formed, the bottom plate is provided with a first through hole, the inner casing surrounds the first through hole, the outer casing surrounds the inner casing, the outer casing and the inner casing are arranged at intervals, and the driving mechanism penetrates through the first through hole and is connected with the reaction cup turntable so as to drive the reaction cup turntable to rotate.
12. The measurement chamber of claim 1, further comprising a drive mechanism, the bottom plate is provided with a first through hole, a fourth annular boss and a fifth annular boss are sequentially formed on the bottom plate along the radial direction of the first through hole, the fourth annular boss is formed by the upward protruding extension of the outer edge of the bottom plate, the central axes of the fifth annular boss, the fourth annular boss and the first through hole are the same, the inner diameter of the fifth annular boss is smaller than that of the fourth annular boss, a first accommodating groove is formed between the fourth annular boss and the fifth annular boss, a second containing groove is formed between the fifth annular boss and the first through hole, the bottom of the shell and the reaction cup turntable is positioned in the first containing groove, the bottom of the inner shell is positioned in the second accommodating groove, and the driving mechanism penetrates through the first through hole to be connected with the reaction cup turntable so as to drive the reaction cup turntable to rotate.
13. The measuring chamber of claim 1, wherein the bottom plate, the outer housing, the inner housing, and the upper cover form a dark chamber, the cuvette carousel comprises a top plate and a peripheral wall, the upper cover is positioned above the top plate, the top plate comprises a first surface and a second surface that are oppositely disposed, the peripheral wall is positioned on the first surface, the peripheral wall surrounds a central axis of the cuvette carousel to form the cuvette carousel cavity, the second surface faces the upper cover, the second surface is in clearance fit with the upper cover, and the inner housing is positioned in the cuvette carousel cavity.
14. The measuring chamber according to claim 13, wherein a first blind hole is formed in the center of the bottom surface of the cover plate of the upper cover, the center of the bottom surface of the first blind hole is sequentially and downwardly protruded to form a first round table and a second round table, the diameter of the first round table is larger than that of the second round table, a limiting step is formed at the joint of the first round table and the second round table, a stepped hole is formed in the center of the second surface of the reaction cup turntable, the stepped hole comprises a large hole and a small hole, the large hole is located above the small hole, a stepped surface is formed at the joint of the large hole and the small hole, a bearing is arranged in the large hole, the second round table is sleeved in the bearing, and the bearing is axially limited between the limiting step and the stepped surface.
15. The measuring chamber according to claim 14, wherein the second surface of the top plate of the cuvette carousel protrudes at the periphery of the large hole to form a second ring, and the second ring is sleeved in the first blind hole.
16. The measuring chamber according to claim 1, wherein the bottom plate is provided with a grounding assembly, the grounding assembly is located in the inner cavity of the reaction cup turntable, the top of the grounding assembly is provided with a spring plate capable of being elastically bent downwards, and the spring plate is pre-pressed between the top plate of the reaction cup turntable and the grounding assembly.
17. A chemiluminescent detector comprising the measurement chamber of any one of claims 1 to 16.
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| CN110044888B (en) * | 2019-04-25 | 2024-08-30 | 壹妙芯(厦门)科技有限公司 | Portable immunity analyzer |
| CN110006825B (en) * | 2019-05-14 | 2024-08-23 | 深圳市亚辉龙生物科技股份有限公司 | Light-shielding structure for optical signal detection |
| CN110244037B (en) * | 2019-07-01 | 2024-05-31 | 北京乐普诊断科技股份有限公司 | Chemiluminescent detection module |
| TWI747673B (en) * | 2020-12-18 | 2021-11-21 | 均豪精密工業股份有限公司 | Image capturing device |
| CN115327096B (en) * | 2022-07-20 | 2023-12-08 | 世纪一束(杭州)医学诊断科技有限公司 | Detection disc applied to immunity analyzer |
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