A kind of chemiluminescence detecting
Technical field
The present invention relates to the chemiluminescence immune assay field, relate to a kind of apparatus structure that is used to carry out chemiluminescence detection particularly.
Background technology
Chemiluminescence immune assay comprises two steps; Be immune response and chemiluminescence analysis; Existing chemiluminescence analysis step has through the integrated chemiluminescence reaction measurement mechanism that is arranged in the chemiluminescence immune assay system to be accomplished, and also has the chemiluminescence detector through special use to carry out luminous detection.No matter through which kind of mode, its main detection part comprises chemiluminescence reaction measuring chamber, chemiluminescence reaction substrate container, chemical luminous substrate shower nozzle, photomultiplier assembly and testing circuit etc.Its detection method is; To finish immunoreactive testing sample and put into the chemiluminescence reaction measuring chamber through reaction cup; Airtight chemiluminescence reaction measuring chamber; Through the chemical luminous substrate shower nozzle reaction substrate in the chemiluminescence reaction substrate container is sprayed into and to carry out chemiluminescence reaction in the reaction cup, the photon that photomultiplier assembly that is connected with the chemiluminescence reaction measuring chamber and testing circuit send chemiluminescence reaction detects, thereby realizes chemiluminescence analysis.
Because in the chemiluminescence measuring process, guarantee the height light leakproofness of measuring chamber, the effusion of photon is the key of chemiluminescence detection when preventing chemiluminescence reaction, therefore, becomes an art technology difficult problem for the structural design of chemiluminescence measuring chamber.
Summary of the invention
The present invention provides a kind of reasonable in design for solving above-mentioned technical barrier, and chemiluminescence detecting is accurately measured in chemiluminescence.
The present invention realizes that the technical scheme that goal of the invention adopts is; A kind of chemiluminescence detecting; Comprise chemiluminescence reaction measuring chamber, chemical luminous substrate shower nozzle, photomultiplier assembly and testing circuit; Said chemical luminous substrate shower nozzle is tightly connected said chemiluminescence reaction measuring chamber in order to the chemiluminescence reaction substrate to be provided; Said photomultiplier assembly and testing circuit are tightly connected said chemiluminescence reaction measuring chamber in order to detect photon number, it is characterized in that: the response measurement chamber is provided with the size measuring chamber inlet corresponding with reaction cup; Measuring chamber inlet go up setting can make the sealing of reaction cup posting port inlet cover; Also comprise the rotation inner housing; The rotation inner housing is arranged in the said chemiluminescence reaction measuring chamber; Said rotation inner housing is provided with the accommodation space that is used for ccontaining reaction cup, and said accommodation space is provided with a light-emitting window, the corresponding said photomultiplier assembly in the position of said light-emitting window; Said rotation inner housing is by the coupling shaft driven rotary that is arranged on chemiluminescence reaction measuring chamber bottom, and said coupling shaft is by motor-driven; Also comprise the shield bars that is used for said accommodation space and said measuring chamber entrance side spatial separation; The inboard corresponding said shield bars at said chemiluminescence reaction measuring chamber top is provided with the upper groove that is used for ccontaining said shield bars; Said rotation inner housing end face is provided with the low groove that is used for ccontaining said shield bars; Be provided with a shell fragment in the said low groove, said upper groove and low groove formation one are used for the space of ccontaining said shield bars.
Better, said rotation inner housing is a cylindrical housings, and said accommodation space is provided with two and with the setting of said shield bars rotational symmetry.
Better, said motor adopts synchronous motor, and said synchronous motor is through the said coupling shaft of band drive synchronously.
Better, be provided with a rotating shaft spring between said coupling shaft and the said rotation inner housing.
Better, the bottom of said accommodation space is provided with push rod and tip-lever support, and said push rod is fixed in the said tip-lever support through a spring activity, and the corresponding said measuring chamber inlet in the bottom of said chemiluminescence reaction measuring chamber is provided with a projection.
Better, in said accommodation space, corresponding said light-emitting window is symmetrically arranged with reflector block with the central shaft of said accommodation space.
The invention has the beneficial effects as follows that structural design is more reasonable, chemiluminescence detection is more accurate, operates more convenient.
Description of drawings
Fig. 1, the chemiluminescence detecting structural drawing of embodiment.
Fig. 2, the A-A broken section enlarged drawing of Fig. 1.
Among the figure; 1 chemiluminescence reaction measuring chamber, 2 chemical luminous substrate shower nozzles, 3 photomultiplier assemblies, 4 measuring chambers inlet, 5 inlet cover, 6 rotation inner housings, 61 reaction cup accommodation spaces, 62 light-emitting windows, 63 reflector blocks, 7 shield bars, 71 upper grooves, 72 low grooves, 73 shell fragments, 8 coupling shafts, 9 are with synchronously, 10 rotating shaft springs, 11 push rods, 12 tip-lever supports, 13 springs, 14 projections.
Embodiment
A kind of chemiluminescence detecting; Structural drawing is referring to accompanying drawing 1; Comprise chemiluminescence reaction measuring chamber 1, chemical luminous substrate shower nozzle 2, photomultiplier assembly 3, measuring chamber inlet 4, inlet cover 5, rotation inner housing 6, shield bars 7, coupling shaft 8, be with 9 synchronously, rotating shaft spring 10; Push rod 11, tip-lever support 12, spring 13, projection 14; Chemical luminous substrate shower nozzle 2 is tightly connected chemiluminescence reaction measuring chamber 1 in order to the chemiluminescence reaction substrate to be provided, and photomultiplier assembly 3 is tightly connected chemiluminescence reaction measuring chamber 1 in order to detect photon number;
Wherein, measuring chamber inlet 4 is arranged on the chemiluminescence reaction measuring chamber 1 opposite side with respect to photomultiplier assembly 3, in order to reaction cup being placed theory of evolution luminescence-producing reaction measuring chamber 1,
Inlet cover 5 in order to plugging meter chamber inlet 4, is fixed on the hangers that is arranged on the chemiluminescence reaction measuring chamber 1, can rotate around hangers and realize switch measuring chamber inlet 4;
Rotation inner housing 6; Be arranged in the chemiluminescence reaction measuring chamber 1; Rotation inner housing 6 is designed to cylindrical housings, is provided with 61, two reaction cup accommodation spaces of two reaction cup accommodation spaces that cooperate with reaction cup 61 and is symmetrical set in the end face center of circle of rotation inner housing 6 relatively; Be provided with light-emitting window 62 in the reaction cup accommodation space 61, the corresponding photomultiplier assembly 3 in the position of light-emitting window 62;
Rotation inner housing 6 its lower ends are connected with coupling shaft 8, and coupling shaft 8 connects through bearing is with 9 synchronously, are with 9 synchronously by synchronous motor drive and then 8 rotations of drive coupling shaft; During detection; Synchronous motor drives is with 9 to rotate synchronously, and then 6 rotations of driven in rotation inner housing, is provided with between coupling shaft 8 and the rotation inner housing 6 and also is provided with rotating shaft spring 10; Under the effect of rotating shaft spring 10, but rotate inner housing 6 elastic displacement in the axial direction;
Shield bars 7; Cross the end face center of circle that rotates inner housing 6 and be provided with, referring to accompanying drawing 2, the inboard corresponding shield bars 7 at chemiluminescence reaction measuring chamber 1 top is provided with upper groove 71; The corresponding shield bars 7 in the top of rotation inner housing 6 is provided with low groove 72; Be provided with a shell fragment 73 in the low groove 72, upper groove 71 and low groove 72 formation one are used for the space of ccontaining shield bars 7, when shield bars 7 rotates to upper groove 71 positions along with rotation inner housing 6; Under effect, snap in upper groove 71 at shell fragment 73; Chemiluminescence reaction measuring chamber 1 is divided into two, and another reaction cup accommodation space of reaction cup accommodation space that will dock with photomultiplier assembly 3 and said measuring chamber inlet side is isolated, and escapes into another reaction cup accommodation space 61 with the reaction cup accommodation space 61 that prevents to dock with photomultiplier assembly 3 photon when the chemiluminescence reaction; Simultaneously; In order to make photon focus on photomultiplier assembly 3, in reaction cup accommodation space 61, corresponding said light-emitting window 62 is symmetrically arranged with reflector block 63 with the central shaft of said accommodation space 61.
For the convenient reaction cup of taking out, be provided with push rod 11 and tip-lever support 12 in the bottom of reaction cup accommodation space, push rod 11 is fixed in the tip-lever support 12 through spring 13 activities, and the corresponding measuring chamber inlet 4 in the bottom of chemiluminescence reaction measuring chamber 1 is provided with a projection 14.
When the said chemiluminescence detecting of application present embodiment detects, open inlet cover 5 earlier, reaction cup to be measured is put into the reaction cup accommodation space 61 of chemiluminescence reaction measuring chamber 1 by measuring chamber inlet 4; Close inlet cover 5 then, start the synchronous motor switch, synchronous motor driven rotary inner housing 6 begins to rotate; Reaction cup in the reaction cup accommodation space 61 is when just beginning to rotate, and push rod 13 leaves arc bump 14 and drops to chemiluminescence reaction measuring chamber 1 bottom under the effect of spring, behind half circumference of synchronous motor driven rotary inner housing 6 rotations; The light-emitting window 62 of reaction cup accommodation space 61 is aimed at photomultiplier assembly 3, and synchronous motor stops operating, at this moment; Shield bars 7 snaps in upper groove 71 under the effect of shell fragment 73, enter the mouth another reaction cup accommodation space of 4 one sides of reaction cup accommodation space that will dock with photomultiplier assembly 3 and measuring chamber is isolated, and in reaction cup, adds the chemiluminescence reaction substrate to carry out luminescence-producing reaction by chemical luminous substrate shower nozzle 2 then; Photomultiplier assembly 3 and testing circuit carry out photon detection; At last, synchronous motor driven rotary inner housing 6 continues half circumference of rotation, and reaction cup turns to the position of corresponding measuring chamber inlet 4; At this moment; Push rod 11 rises along projection 14, reaction cup is pushed up measuring chamber inlet 4 go out, and the person takes out reaction cup with handled easily.
What should explain at last is: above embodiment only in order to the explanation the present invention and and unrestricted technical scheme described in the invention; Therefore, it will be understood by those of skill in the art that still and can make amendment or be equal to replacement the present invention although this instructions has carried out detailed explanation with reference to each above-mentioned embodiment to the present invention; And all do not break away from the technical scheme and the improvement thereof of the spirit and scope of the present invention, and it all should be encompassed in the claim scope of the present invention.