CN110244037B - Chemiluminescent detection module - Google Patents
Chemiluminescent detection module Download PDFInfo
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- CN110244037B CN110244037B CN201910583118.4A CN201910583118A CN110244037B CN 110244037 B CN110244037 B CN 110244037B CN 201910583118 A CN201910583118 A CN 201910583118A CN 110244037 B CN110244037 B CN 110244037B
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- 238000001514 detection method Methods 0.000 title claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 48
- 239000002699 waste material Substances 0.000 claims abstract description 41
- 238000012795 verification Methods 0.000 claims abstract description 24
- 230000003287 optical effect Effects 0.000 claims abstract description 8
- 239000000758 substrate Substances 0.000 claims description 23
- 230000001360 synchronised effect Effects 0.000 claims description 11
- 238000005213 imbibition Methods 0.000 claims description 5
- 238000007599 discharging Methods 0.000 claims 2
- 230000000903 blocking effect Effects 0.000 abstract description 4
- 101000623895 Bos taurus Mucin-15 Proteins 0.000 description 10
- 238000002038 chemiluminescence detection Methods 0.000 description 10
- 238000010586 diagram Methods 0.000 description 6
- 230000001052 transient effect Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 206010034960 Photophobia Diseases 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000007306 turnover Effects 0.000 description 1
Abstract
The invention discloses a chemiluminescent detection module which is provided with an optical path component, a verification component, a darkroom mechanism and a waste liquid suction mechanism; the light path component is provided with a photomultiplier, a light guide rod and a light condensing cup, the photomultiplier is connected with the light guide rod, and the light condensing cup is arranged at the side part of the light guide rod; the verification assembly is provided with a lens, a diaphragm, an attenuation sheet and a light source, the lens is connected to the outer side of the light gathering cup, the diaphragm is connected to the outer side of the lens, the attenuation sheet is connected to the outer side of the diaphragm, the light source is connected to the outer side of the attenuation sheet, and light emitted by the light source is projected to the photomultiplier through the attenuation sheet, the diaphragm and the lens in sequence; the darkroom mechanism is arranged at the outer side of the checking assembly and used for blocking external environment light rays from projecting to the checking assembly; the waste liquid absorbing mechanism is connected with the darkroom mechanism and absorbs the waste liquid in the light-gathering cup. According to the technical scheme, the reaction cup is quickly replaced, light emitted by a detection object can be effectively converged on the photomultiplier, external stray light interference is avoided, and the accuracy of a detection result is high.
Description
Technical Field
The invention relates to a chemiluminescent detection module, and belongs to the technical field of in-vitro diagnostic instruments.
Background
The chemiluminescent immunoassay technology is widely applied to the field of in-vitro diagnosis due to higher accuracy. The principle is that a sample to be detected is placed in a reaction cup, the reaction cup is placed in a detection darkroom, a substrate is filled in, the sample emits light, photons are projected onto a photomultiplier through a light path to be received and detected, and therefore chemiluminescence analysis is achieved. Because chemiluminescence itself characteristics, the light that sends out is transient light or glow, so traditional technical scheme is difficult to guarantee to detect the photophobia of darkroom, and the interference of often receiving external parasitic light is the close of chemiluminescence analyzer to detection module can't cooperate fast with the tongs module, and the replacement speed of reaction cup is slow, and the transient light or the glow that the detection thing sent can not effectively assemble, influences the testing result, and what need is urgent is a chemiluminescence detection technical scheme.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a chemiluminescent detection module which can realize the rapid replacement of a reaction cup, and can effectively collect instantaneous light or glow emitted by a detection object on a photomultiplier tube, and simultaneously avoid the entry and interference of external stray light.
The technical scheme for solving the technical problems is as follows: a chemiluminescent detection module comprises an optical path component, a verification component, a darkroom mechanism and a waste liquid suction mechanism; the light path component comprises a photomultiplier, a light guide rod and a light condensing cup, wherein the photomultiplier is connected with the light guide rod, and the light condensing cup is arranged on the side part of the light guide rod; the verification assembly comprises a lens, a diaphragm, an attenuation sheet and a light source, wherein the lens is connected to the outer side of the light gathering cup, the diaphragm is connected to the outer side of the lens, the attenuation sheet is connected to the outer side of the diaphragm, the light source is connected to the outer side of the attenuation sheet, and light emitted by the light source sequentially passes through the attenuation sheet, the diaphragm and the lens and is projected to the photomultiplier; the darkroom mechanism is arranged on the outer side of the verification assembly and used for blocking external environment light rays from projecting to the verification assembly; the waste liquid absorbing mechanism is connected with the darkroom mechanism and absorbs the waste liquid in the light-gathering cup.
As the preferable scheme of chemiluminescence detection module, darkroom mechanism includes mounting panel and light screen, the light screen passes through first labyrinth structure and connects the lateral part of mounting panel, the lateral part of mounting panel is equipped with first guide rail, and the top of mounting panel is connected with the upper cover, the light screen is connected first guide rail.
As the preferable scheme of chemiluminescence detection module, the light screen is connected with the traction spring, the traction spring is connected in the lateral part of mounting panel.
As the preferential scheme of chemiluminescence detection module, the lateral part of mounting panel is equipped with the second guide rail, upper cover are connected with the saucer of placing the reaction cup through the second labyrinth structure, the saucer is connected with the drive belt, the drive belt is connected with and is fixed in the first motor of mounting panel lateral part.
As the preferable scheme of chemiluminescence detection module, check-up subassembly sets up the inside of saucer, saucer end connection has the circuit board, check-up subassembly's light source is connected on the circuit board.
As the preferable scheme of chemiluminescence detection module, the top of darkroom mechanism is equipped with the substrate and connects, substrate connects intercommunication reaction cup, and substrate connects and disposes the substrate pipeline, substrate pipeline is connected with rotatory plunger pump.
As the preferable scheme of chemiluminescence detection module, waste liquid suction means includes second motor, third guide rail, hold-in range and absorbs waste liquid subassembly, the second motor is connected the below of darkroom mechanism, the third guide rail is connected the lateral part of darkroom mechanism, the hold-in range passes through the band pulley and connects the lateral part of darkroom mechanism, the band pulley is connected the second motor, it connects to inhale waste liquid subassembly the hold-in range.
As the preferable scheme of chemiluminescence detection module, the hold-in range is connected with the adapter, the adapter is followed the second guide rail slides, inhale waste liquid subassembly and connect the top of adapter.
As the preferable scheme of chemiluminescence detection module, inhale waste liquid subassembly includes lifting rod, xarm, needle file and imbibition needle, the lifting rod is connected the top of adapter piece, the xarm is connected the top of lifting rod, the xarm extends to the top of darkroom mechanism, the needle file is connected the terminal of xarm, imbibition needle is connected the below of needle file.
As the preferable scheme of chemiluminescence detection module, the imbibition needle is connected with the fluid-discharge tube, the fluid-discharge tube is connected with the suction pump.
The beneficial effects of the invention are as follows: the device is provided with an optical path component, a verification component, a darkroom mechanism and a waste liquid suction mechanism; the light path component is provided with a photomultiplier, a light guide rod and a light condensing cup, the photomultiplier is connected with the light guide rod, and the light condensing cup is arranged at the side part of the light guide rod; the verification assembly is provided with a lens, a diaphragm, an attenuation sheet and a light source, the lens is connected to the outer side of the light gathering cup, the diaphragm is connected to the outer side of the lens, the attenuation sheet is connected to the outer side of the diaphragm, the light source is connected to the outer side of the attenuation sheet, and light emitted by the light source is projected to the photomultiplier through the attenuation sheet, the diaphragm and the lens in sequence; the darkroom mechanism is arranged at the outer side of the checking assembly and used for blocking external environment light rays from projecting to the checking assembly; the waste liquid absorbing mechanism is connected with the darkroom mechanism and absorbs the waste liquid in the light-gathering cup. According to the technical scheme, the reaction cup can be replaced quickly, instantaneous light or glow emitted by a detected object can be effectively converged on the photomultiplier, meanwhile, entering and interference of external stray light are avoided, and accuracy of detection results is high.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It will be apparent to those of ordinary skill in the art that the drawings in the following description are exemplary only and that other implementations can be obtained from the extensions of the drawings provided without inventive effort.
The structures, proportions, sizes, etc. shown in the present specification are shown only for the purposes of illustration and description, and are not intended to limit the scope of the invention, which is defined by the claims, so that any structural modifications, changes in proportions, or adjustments of sizes, which do not affect the efficacy or the achievement of the present invention, should fall within the ambit of the technical disclosure.
FIG. 1 is a schematic diagram of the external structure of a chemiluminescent detection module according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of an internal structure of a chemiluminescent detection module according to an embodiment of the present invention;
FIG. 3 is a schematic diagram of the structure of the optical path component and the calibration component of the chemiluminescent detection module according to the embodiment of the present invention;
FIG. 4 is a schematic diagram of the inside of a darkroom mechanism of a chemiluminescent detection module according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a structure of a shielding plate and a mounting plate of a chemiluminescent detection module according to an embodiment of the present invention;
Fig. 6 is a schematic diagram of a matching structure of a cup holder and a light shielding plate of a chemiluminescent detection module according to an embodiment of the present invention.
In the figure, I, an optical path component; II. A verification assembly; III, a darkroom mechanism; IV, a waste liquid sucking mechanism;
1. A photomultiplier tube; 2. a light guide bar; 3. a light gathering cup; 4. a lens; 5. a diaphragm; 6. an attenuation sheet; 7. a light source; 8. a mounting plate; 9. a light shielding plate; 10. a first labyrinth structure; 11. a first guide rail; 12. a pulling spring; 13. a second guide rail; 14. a second labyrinth structure; 15. a cup holder; 16. a drive belt; 17. a first motor; 18. a circuit board; 19. a substrate linker; 20. a second motor; 21. a third guide rail; 22. a synchronous belt; 23. a transfer block; 24. a lifting rod; 25. a cross arm; 26. a needle stand; 27. a liquid suction needle; 28. a reaction cup; 29. and (5) an upper cover.
Detailed Description
In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1 and 2, a chemiluminescent detection module is provided, which comprises an optical path component I, a verification component II, a darkroom mechanism III and a waste liquid absorbing mechanism IV; the light path component I comprises a photomultiplier 1, a light guide rod 2 and a light condensing cup 3, wherein the photomultiplier 1 is connected with the light guide rod 2, and the light condensing cup 3 is arranged on the side part of the light guide rod 2; the verification component II comprises a lens 4, a diaphragm 5, an attenuation sheet 6 and a light source 7, wherein the lens 4 is connected to the outer side of the light gathering cup 3, the diaphragm 5 is connected to the outer side of the lens 4, the attenuation sheet 6 is connected to the outer side of the diaphragm 5, the light source 7 is connected to the outer side of the attenuation sheet 6, and light emitted by the light source 7 is projected to the photomultiplier 1 through the attenuation sheet 6, the diaphragm 5 and the lens 4 in sequence; the darkroom mechanism III is arranged on the outer side of the verification component II and blocks external environment light rays from projecting to the verification component II; the waste liquid absorbing mechanism IV is connected with the darkroom mechanism III, and absorbs waste liquid in the light-gathering cup 3.
Referring to fig. 5 and 6, in one embodiment of the chemiluminescent detection module, the darkroom mechanism III includes a mounting plate 8 and a light shielding plate 9, the light shielding plate 9 is connected to a side portion of the mounting plate 8 through a first labyrinth structure 10, a first guide rail 11 is disposed on the side portion of the mounting plate 8, an upper cover 29 is connected to a top portion of the mounting plate 8, and the light shielding plate 9 is connected to the first guide rail 11. The light shielding plate 9 is connected with a traction spring 12, and the traction spring 12 is connected to the side part of the mounting plate 8. The lateral part of mounting panel 8 is equipped with second guide rail 13, second guide rail 13 and upper cover 29 are connected with the saucer 15 of placing reaction cup 28 through second labyrinth structure 14, saucer 15 is connected with drive belt 16, drive belt 16 is connected with and is fixed in the first motor 17 of mounting panel 8 lateral part. The first motor 17 can drive the cup holder 15 to move along the second guide rail 13 through the driving belt 16. The first labyrinth structure 10 and the second labyrinth structure 14 are provided with a plurality of turnover structures to realize shielding of external environment light, the light shielding plate 9 can slide on the first guide rail 11, the photomultiplier 1 is shielded in a non-detection state, and the photomultiplier 1 is prevented from being interfered by external stray light.
Referring to fig. 3 and 4, in one embodiment of the chemiluminescent detection module, a verification component II is disposed inside the cup holder 15, a circuit board 18 is connected to an end of the cup holder 15, and the light source 7 of the verification component II is connected to the circuit board 18. The top of the darkroom mechanism III is provided with a substrate joint 19, the substrate joint 19 is communicated with a reaction cup 28, the substrate joint 19 is provided with a substrate pipeline, and the substrate pipeline is connected with a rotary plunger pump (the substrate pipeline and the rotary plunger pump attachment structure are not shown). The substrate connector 19 is used for adding reaction substrates into the reaction cup 28, the light source 7 on the circuit board 18 emits light, the light focusing cup 3 focuses the light emitted by the substrates and is projected onto the photomultiplier 1 through the light guide rod 2.
Referring to fig. 2, in one embodiment of the chemiluminescent detection module, the waste liquid suction mechanism IV includes a second motor 20, a third guide rail 21, a synchronous belt 22 and a waste liquid suction assembly, wherein the second motor 20 is connected below the darkroom mechanism III, the third guide rail 21 is connected to a side portion of the darkroom mechanism III, the synchronous belt 22 is connected to a side portion of the darkroom mechanism III through a belt pulley, the belt pulley is connected to the second motor 20, and the waste liquid suction assembly is connected to the synchronous belt 22. The synchronous belt 22 is connected with an adapter block 23, the adapter block 23 slides along the third guide rail 21, and the waste liquid sucking component is connected above the adapter block 23. The second motor 20 drives the synchronous belt 22 to operate through the belt pulley, and the adapter block 23 moves up and down along the third guide rail 21 when the synchronous belt 22 operates, so that the waste liquid sucking assembly connected with the adapter block 23 moves up and down.
Specifically, the waste liquid absorbing assembly comprises a lifting rod 24, a cross arm 25, a needle seat 26 and a liquid absorbing needle 27, wherein the lifting rod 24 is connected above the adapter block 23, the cross arm 25 is connected to the top of the lifting rod 24, the cross arm 25 extends to the upper part of the darkroom mechanism III, the needle seat 26 is connected to the tail end of the cross arm 25, and the liquid absorbing needle 27 is connected to the lower part of the needle seat 26. The pipette needle 27 is connected to a drain pipe to which a pipette pump (attached structure not shown) is connected. The waste liquid sucked up by the suction needle 27 is discharged by the suction pump through the liquid discharge pipe.
The invention is provided with an optical path component I, a verification component II, a darkroom mechanism III and a waste liquid absorbing mechanism IV; the light path component I is provided with a photomultiplier 1, a light guide rod 2 and a light condensing cup 3, wherein the photomultiplier 1 is connected with the light guide rod 2, and the light condensing cup 3 is arranged on the side part of the light guide rod 2; the verification component II is provided with a lens 4, a diaphragm 5, an attenuation sheet 6 and a light source 7, wherein the lens 4 is connected to the outer side of the light gathering cup 3, the diaphragm 5 is connected to the outer side of the lens 4, the attenuation sheet 6 is connected to the outer side of the diaphragm 5, the light source 7 is connected to the outer side of the attenuation sheet 6, and light emitted by the light source 7 sequentially passes through the attenuation sheet 6, the diaphragm 5 and the lens 4 to be projected to the photomultiplier 1; the darkroom mechanism III is arranged at the outer side of the verification component II and used for blocking external environment light rays from projecting to the verification component II; the waste liquid absorbing mechanism IV is connected with the darkroom mechanism III, and absorbs waste liquid in the light focusing cup 3. According to the technical scheme, in the undetected state, the light shielding plate 9 is pulled by the pulling spring 12 to shield the light guide rod 2 and the photomultiplier 1, when the reaction cup 28 containing an object to be detected is placed in the cup holder 15, the first motor 17 drives the cup holder 15 to move along the second guide rail 13 through the driving belt 16, meanwhile, the first motor is matched with the light shielding plate 9, the light shielding plate 9 is pushed away, substrate begins to be filled through the substrate connector 19, and the photomultiplier 1 records the photon quantity. After the detection is finished, the first motor 17 drives the cup holder 15 to move to a waste liquid sucking position on the second guide rail 13, the second motor 20 drives the waste liquid sucking component to move to the bottom of the reaction cup 28 on the third guide rail 21, the detected waste liquid is sucked away, the second motor 20 drives the waste liquid sucking component to move to a high position on the third guide rail 21, the first motor 17 drives the cup holder 15 to move to a handle position on the second guide rail 13, and the existing handle module picks up the reaction cup 28 and drops to a waste bin. According to the technical scheme, the reaction cup 28 can be replaced quickly, transient light or glow emitted by a detected object can be effectively converged on the photomultiplier 1, meanwhile, entering and interference of external stray light are avoided, and the accuracy of a detection result is high.
The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.
The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.
Claims (8)
1. A chemiluminescent detection module, which is characterized in that,
Comprises an optical path component (I), a verification component (II), a darkroom mechanism (III) and a waste liquid absorbing mechanism (IV); the light path component (I) comprises a photomultiplier (1), a light guide rod (2) and a light condensing cup (3), wherein the photomultiplier (1) is connected with the light guide rod (2), and the light condensing cup (3) is arranged on the side part of the light guide rod (2); the verification assembly (II) comprises a lens (4), a diaphragm (5), an attenuation sheet (6) and a light source (7), wherein the lens (4) is connected to the outer side of the light gathering cup (3), the diaphragm (5) is connected to the outer side of the lens (4), the attenuation sheet (6) is connected to the outer side of the diaphragm (5), the light source (7) is connected to the outer side of the attenuation sheet (6), and light emitted by the light source (7) sequentially passes through the attenuation sheet (6), the diaphragm (5) and the lens (4) to be projected to the photomultiplier (1); the darkroom mechanism (III) is arranged at the outer side of the verification assembly (II), and the darkroom mechanism (III) blocks external environment light from projecting to the verification assembly (II); the waste liquid absorbing mechanism (IV) is connected with the darkroom mechanism (III), and absorbs waste liquid in the light-gathering cup (3);
the darkroom mechanism (III) comprises a mounting plate (8) and a light shielding plate (9), the light shielding plate (9) is connected to the side part of the mounting plate (8) through a first labyrinth structure (10), a first guide rail (11) is arranged on the side part of the mounting plate (8), an upper cover (29) is connected to the top of the mounting plate (8), and the light shielding plate (9) is connected with the first guide rail (11);
The shading plate (9) is connected with a traction spring (12), and the traction spring (12) is connected to the side part of the mounting plate (8);
The lateral part of mounting panel (8) is equipped with second guide rail (13), upper cover (29) are connected with saucer (15) of placing reaction cup (28) through second labyrinth (14), and check-up subassembly (II) set up the inside of saucer (15).
2. The chemiluminescent detection module of claim 1 wherein the detection module comprises,
The cup stand (15) is connected with a driving belt (16), and the driving belt (16) is connected with a first motor (17) fixed on the side part of the mounting plate (8).
3. A chemiluminescent detection module of claim 2 wherein the detection module comprises,
The end part of the cup stand (15) is connected with a circuit board (18), and the light source (7) of the verification assembly (II) is connected to the circuit board (18).
4. The chemiluminescent detection module of claim 1 wherein the detection module comprises,
The top of darkroom mechanism (III) is equipped with substrate joint (19), substrate joint (19) intercommunication reaction cup (28), substrate joint (19) are furnished with the substrate pipeline, the substrate pipeline is connected with rotatory plunger pump.
5. The chemiluminescent detection module of claim 1 wherein the detection module comprises,
The waste liquid absorbing mechanism (IV) comprises a second motor (20), a third guide rail (21), a synchronous belt (22) and a waste liquid absorbing component, wherein the second motor (20) is connected to the lower portion of the darkroom mechanism (III), the third guide rail (21) is connected to the side portion of the darkroom mechanism (III), the synchronous belt (22) is connected to the side portion of the darkroom mechanism (III) through a belt wheel, the belt wheel is connected to the second motor (20), and the waste liquid absorbing component is connected to the synchronous belt (22).
6. The chemiluminescent detection module of claim 5 wherein,
The synchronous belt (22) is connected with an adapter block (23), the adapter block (23) slides along the second guide rail (13), and the waste liquid sucking component is connected above the adapter block (23).
7. The chemiluminescent detection module of claim 6 wherein the detection module comprises,
Waste liquid absorbing assembly includes lifting rod (24), xarm (25), needle file (26) and imbibition needle (27), lifting rod (24) are connected the top of adapter piece (23), xarm (25) are connected the top of lifting rod (24), xarm (25) extend to the top of darkroom mechanism (III), needle file (26) are connected the end of xarm (25), imbibition needle (27) are connected the below of needle file (26).
8. The chemiluminescent detection module of claim 7 wherein the detection module comprises,
The liquid sucking needle (27) is connected with a liquid discharging pipe, and the liquid discharging pipe is connected with a liquid sucking pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910583118.4A CN110244037B (en) | 2019-07-01 | Chemiluminescent detection module |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910583118.4A CN110244037B (en) | 2019-07-01 | Chemiluminescent detection module |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110244037A CN110244037A (en) | 2019-09-17 |
| CN110244037B true CN110244037B (en) | 2024-05-31 |
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