CN112881368A - Chemiluminescence immunity analyzer - Google Patents
Chemiluminescence immunity analyzer Download PDFInfo
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- CN112881368A CN112881368A CN202110280696.8A CN202110280696A CN112881368A CN 112881368 A CN112881368 A CN 112881368A CN 202110280696 A CN202110280696 A CN 202110280696A CN 112881368 A CN112881368 A CN 112881368A
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- cover plate
- reaction cup
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- incubation
- immunoassay analyzer
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
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Abstract
The invention provides a chemiluminescence immunoassay analyzer which is provided with a waste liquid extraction port (6) and a cleaning port (7), and comprises a reaction cup (4), an upper shading part (10) and a side shading part (20), wherein the upper shading part (10) comprises an injection cover plate (11), the side shading part (20) comprises a reaction cup turntable (22) and a small light blocking cover plate (21), the reaction cup turntable (22) is provided with a turntable through hole which is used for the reaction cup (4) to pass through and penetrates in the axial direction (A), the small light blocking cover plate (21) is cylindrical, one end of the small light blocking cover plate (21) in the axial direction (A) is sleeved on the injection cover plate (11), and the other end of the small light blocking cover plate (21) abuts against the reaction cup turntable (22) and surrounds the turntable through hole. The mechanism has the advantages of small volume, low cost, compact structure, reliable operation and effective shielding of outside light from entering the detection system.
Description
Technical Field
The invention relates to an immunoassay analyzer, in particular to a chemiluminescence immunoassay analyzer.
Background
The chemiluminescence immunoassay analyzer adopts chemiluminescence immunoassay technology to carry out qualitative or quantitative detection on analytes in human serum, blood plasma or other body fluid samples.
Chemiluminescence immunoassay comprises two systems of immunoassay and chemiluminescence immunoassay. The immunoassay system directly marks chemiluminescent substances or enzymes on antigens or antibodies as markers, and forms antigen-antibody immune complexes through the reaction of the antigens and the antibodies. The chemiluminescence analysis system is characterized in that after the immune reaction is finished, a luminous substrate of an oxidant or an enzyme is added, a chemiluminescent substance is oxidized by the oxidant to form an intermediate in an excited state, photons are emitted to release energy so as to return to a stable ground state, and the luminous intensity can be detected by a luminous signal measuring instrument (a photomultiplier). According to the relation between the chemiluminescence marker and the luminescence intensity, the content of the detected object can be calculated by using a standard curve.
The working principle of the chemiluminescence immunoassay analyzer is as follows: the sample adding module adds the sample and the reagent into the reaction cup, after the sample and the reagent in the reaction cup are subjected to incubation reaction in the incubation zone, the reaction carrier is adsorbed by a magnetic field mode, the unbound sample and the unbound reagent are washed away, and only the complex formed by the reaction is reserved. Then adding hydrogen peroxide, fully mixing, and moving to a measurement area. The light blocking mechanism raises the light blocking sheet to eliminate outside stray light, sodium hydroxide is added into the reaction cup, the photomultiplier is started to capture photons generated by the reaction, the number of photons (luminous intensity) is measured by the photomultiplier, and the luminous intensity is converted into the concentration of an analyte by software.
The chemical luminous reaction reactant emits light and the light is weak (especially when measuring low concentration substance), so the interference of external light to the chemical luminous reaction reactant is avoided when the photomultiplier is used for detection, and the chemical luminous reaction reactant is shielded from light by a dark room.
The darkroom mechanism of the existing chemiluminescence immunoassay analyzer is complex, the cost is high, and the failure probability is high.
Chemiluminescence immune analyzer is usually used for point of care (POCT) equipment, and the POCT equipment is characterized by small volume and compact structure. Therefore, the photomultiplier has a waste liquid suction port and a cleaning port in close proximity to the light reading port, and the aperture of each of these openings is not less than 6 mm. The existence of these heavy-calibre through-holes makes external light, especially highlight, can follow these mouths and get into inside the reaction dish, through the reflection of light, through the gap between the spare part, finally reaches photomultiplier's detection face to influence the accuracy of test result.
Disclosure of Invention
It is an object of the present invention to overcome or at least alleviate the above-mentioned deficiencies of the prior art and to provide a chemiluminescent immunoassay analyzer.
The invention provides a chemiluminescence immunoassay analyzer, which is provided with a waste liquid extraction port and a cleaning port and comprises a reaction cup, an upper shading part and a side shading part, wherein the upper shading part and the side shading part can shade light from the waste liquid extraction port and the cleaning port to the reaction cup,
the upper shading part comprises an injection cover plate arranged above the reaction cup,
the side shading part comprises a reaction cup turntable which can rotate around the central axis of the side shading part and a light blocking small cover plate which is arranged between the liquid injection cover plate and the reaction cup turntable,
the reaction cup turntable is provided with a turntable through hole which is used for the reaction cup to pass through and is communicated in the axial direction, the light blocking small cover plate is in a cylindrical shape, one end of the light blocking small cover plate in the axial direction is sleeved with the liquid injection cover plate, and the other end of the light blocking small cover plate is abutted against the reaction cup turntable and surrounds the turntable through hole.
Preferably, two concentric cylindrical structures are formed at one end of the liquid injection cover plate facing the reaction cup turntable, and the small light blocking cover plate is embedded between the two cylindrical structures.
Preferably, the side shading part further comprises an upper cover plate, an inner cavity and an upper opening are formed in the upper cover plate, the inner cavity of the upper cover plate accommodates the reaction cup turntable, the upper opening of the upper cover plate is matched with the outer peripheral surface of the light blocking small cover plate, and the liquid injection cover plate is fixed on the upper cover plate.
Preferably, the chemiluminescent immunoassay analyzer further comprises a lower light shield comprising an incubation tray,
the incubation tray accommodates the portion of the reaction cup that extends out of the reaction cup carousel,
the inner peripheral area of the end face of the incubation disc facing the reaction cup rotating disc partially protrudes towards the reaction cup rotating disc to form an annular primary step, and the primary step extends into the inner cavity of the upper cover plate.
Preferably, the chemiluminescence immunoassay analyzer further comprises a photomultiplier and a light guide rod, the lower shading part further comprises a light guide rod sleeve,
one end of the light guide rod penetrates through the side wall of the incubation disc to extend to the reaction cup, the other end of the light guide rod extends out of the incubation disc to be connected with the photomultiplier,
the light guide rod sleeve wraps the portion of the light guide rod exposed outside of the incubation tray.
Preferably, the lower light-shielding member further comprises two moving light-blocking sheets,
the incubation tray comprises an incubation well in the shape of a ring groove for receiving a portion of the reaction cups,
the movable light blocking sheet can move along the axial direction to enter or leave the incubation groove, and when the movable light blocking sheet enters the incubation groove, the two movable light blocking sheets respectively shield light on two circumferential sides of the reaction cup.
Preferably, the thickness of the moving light-blocking sheet is 3mm to 5 mm.
Preferably, the size of the moving light-blocking sheet is larger than that of the incubation groove in a radial direction of the incubation tray, and the incubation tray is formed with a groove penetrating in an axial direction to accommodate the moving light-blocking sheet.
Preferably, the movable light-blocking sheet is driven by a driving mechanism, the driving mechanism includes a screw motor, a screw and a linear guide rail, the screw motor provides driving force, and the linear guide rail provides guidance for the movable light-blocking sheet.
Preferably, the upper shading part further comprises a water pipe connector and an injection water pipe, the water pipe connector is connected with the upper part of the injection cover plate, and the injection water pipe sequentially penetrates through the water pipe connector and the injection cover plate and extends to the position above the reaction cup.
The chemiluminescence immunoassay analyzer has the advantages of small volume, low cost, compact structure, reliable operation and effective shielding of outside light from entering a detection system.
Drawings
FIG. 1 is a schematic diagram of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.
Fig. 2 is a sectional view of a partial structure of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.
Fig. 3 is a partially enlarged schematic view of fig. 2.
Fig. 4 is a cross-sectional view perpendicular to the axial direction of a partial structure of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention.
Description of reference numerals:
10 an upper light shielding member; 11 liquid injection cover plate; 12 water pipe joints; 13 liquid injection water pipe;
20 side light shielding members; 21 light blocking small cover plate; 22 reaction cup rotating disc; 23, an upper cover plate;
30 lower light shielding member; 31 incubation tray; 311, a step; 32 moving the light barrier; 33 a light guide rod sleeve;
4, a reaction cup; 5 a photomultiplier tube; 51 a light guide rod; 6 a waste liquid pumping port; 7 cleaning the opening;
80 a drive mechanism; 81 lead screw motor; 82, a screw rod; 83 linear guide rail.
Detailed Description
Exemplary embodiments of the present invention are described below with reference to the accompanying drawings. It should be understood that the detailed description is intended only to teach one skilled in the art how to practice the invention, and is not intended to be exhaustive or to limit the scope of the invention.
Fig. 1 is an overall schematic view of a chemiluminescence immunoassay analyzer (hereinafter also referred to simply as an analyzer) according to an embodiment of the present invention, in which a liquid extraction and waste port 6 and a plurality of cleaning ports 7 are provided in close proximity to a light reading port of a photomultiplier 5, and the analyzer according to the present invention prevents external light from entering a gap between a cuvette 4 and a detection surface of the photomultiplier 5 from these ports and affecting the accuracy of a test result.
Fig. 2 to 4 are partial sectional views of a chemiluminescent immunoassay analyzer according to one embodiment of the present invention at a light-shielding darkroom composed of three parts, an upper light-shielding member 10, a side light-shielding member 20 and a lower light-shielding member 30.
The upper light shielding member 10 includes components such as a liquid filling cover plate 11, a water pipe connector 12, and a liquid filling water pipe 13.
The side light shielding member 20 includes a light shielding small cover 21, a cuvette turntable 22, an upper cover 23, and the like.
The lower light shield 30 includes components such as an incubation tray 31, a moving light barrier 32, and a light guide rod sleeve 33.
All the parts are made of opaque materials, so that no light can penetrate through the parts.
The various components of the darkroom arrangement of the mechanism of the present invention are described below with reference to figures 2 to 4.
The liquid injection cover plate 11 is arranged right above the reaction cup 4, a threaded hole is formed in the upper portion of the liquid injection cover plate 11, and the water pipe joint 12 extends into the threaded hole to be in threaded connection with the liquid injection cover plate 11. The liquid injection cover plate 11 and the water pipe joint 12 are further provided with through holes for the liquid injection water pipe 13 to pass through, and the liquid injection water pipe 13 sequentially penetrates through the water pipe joint 12 and the liquid injection cover plate 11 from top to bottom so as to extend to the position right above the reaction cup 4.
The upper light-shielding member 10 including the liquid-filling cover plate 11, the water pipe connector 12 and the liquid-filling water pipe 13 can shield the top of the reaction cup 4 from the outside light.
The reaction cup rotating disc 22 can rotate around the central axis thereof, a rotating disc through hole which is through in the axial direction A is arranged on the reaction cup rotating disc 22 and is used for the reaction cup 4 to pass through, the rotating disc through hole is a stepped hole, the large end of the stepped hole is arranged above and is matched with the light blocking small cover plate 21, the small end of the stepped hole is arranged below and is matched with the reaction cup 4, and therefore the reaction cup rotating disc 22 can drive the reaction cup 4 to rotate together when rotating around the central axis thereof.
The light blocking small cover plate 21 is cylindrical, one end of the light blocking small cover plate 21 in the axial direction A is sleeved on the liquid injection cover plate 11, and the other end of the light blocking small cover plate abuts against the upper surface of the reaction cup turntable 22 and surrounds the turntable through hole. Preferably, the light blocking small cover plate 21 is abutted against the upper surface of the reaction cup rotating plate 22 by means of self gravity, so that the light blocking small cover plate can be attached to the upper surface of the reaction cup rotating plate 22 without other gravity mechanisms or clamping mechanisms. The outer peripheral surface of the light-blocking small cover 21 is also fitted with the upper cover 23 to block the entrance of external light.
The upper cover plate 23 is in a cover shape and includes a top surface, a side wall and an inner cavity surrounded by the top surface and the side wall, and the top surface of the upper cover plate 23 is provided with a plurality of openings for forming a cleaning opening, a waste liquid extraction opening, a liquid injection opening and the like.
In the chemiluminescent immunoassay analyzer shown in fig. 2 to 3, an opening formed in the upper cover plate 23 is used to cooperate with the light blocking small cover plate 21 to form a liquid pouring port. The top surface of the upper cover plate 23 is also fixedly connected with an injection cover plate 11, a reaction cup rotating disc 22 is accommodated in the inner cavity of the upper cover plate 23, and an incubation disc 31 is also partially accommodated in the accommodating cavity of the upper cover plate 23.
The small light blocking cover 21 in the side light blocking member 20 can block the external light transmitted from the gap between the cuvette turntable 22 and the upper cover 23.
The incubation disk 31 is disposed below the cuvette carousel 22, and an annular incubation groove for accommodating the cuvette 4 is axially A provided in the incubation disk 31. The side wall of the incubation plate 31 is horizontally provided with a through hole matched with the light guide rod 51. The upper cover plate 23 is partly pressed against the peripheral area of the incubation plate 31. The inner peripheral area of the incubation plate 31 is partially protruded toward the cuvette carousel 22 to form a first step 311, so that the incubation plate 31 is partially received in the receiving chamber of the upper cover plate 23. The above-mentioned cooperation of the upper cover plate 23 and the incubation plate 31 also helps to shield the outside light.
The incubation groove of the incubation plate 31 is a circular groove, so that the incubation groove is open on both circumferential sides of the reaction cup 4, and the present invention is provided with one moving light blocking sheet 32 on each of both circumferential sides of the portion of the reaction cup 4 received in the incubation groove. The moving light-blocking sheet 32 is disposed adjacent to the reaction cup 4 in the circumferential direction.
Referring to fig. 4, in the radial direction R of the incubation disc 31, the size of the moving light barrier 32 is larger than the size of the incubation slot, and the moving light barrier 32 can axially enter the incubation disc 31 through the through slot provided on the incubation disc 31. The moving light-blocking plate 32 surrounds the reaction cups 4 together with the incubation plate 31, so that the moving light-blocking plate 31 blocks light transmitted from the inside of the incubation tank.
Preferably, the thickness of the moving light-blocking sheet is 3mm to 5 mm.
Preferably, the moving light-blocking sheet 31 is driven by a driving mechanism 80, and the driving mechanism 80 includes a lead screw motor 81, a lead screw 82, and a linear guide 83. The linear guide 83 provides a guide for the moving flag 32 by means of the driving force provided by the lead screw motor 81, so that the moving flag 32 can enter the incubation tray 31 axially.
One end of the light guide rod 51 extends to the reaction cup 4 through the through hole on the sidewall of the incubation tray 31, the other end of the light guide rod 51 is connected with the photomultiplier 5, and the portion of the light guide rod 51 exposed outside the sidewall of the incubation tray 31 is wrapped by the light guide rod sleeve 33.
The lower light-shielding member 30 composed of the incubation plate 31, the movable light-blocking plate 32 and the light guide rod sleeve 33 can shield the photomultiplier tube 4 from the external light near the light-reading port.
The invention has at least one of the following advantages:
(i) the mechanism of the invention shields the periphery of the reaction cup by the upper shielding part 10, the side shielding part 20 and the lower shielding part 30, ensures the minimum quantity of the entering stray light and improves the accuracy of the test result.
(ii) The mechanism adopts the light-blocking small cover plate 21, and not only can replace the attaching mode of the up-and-down movement of a motor through the precise long-distance matching in the vertical direction and the seamless matching pressed on the reaction cup turntable 22 by means of gravity, but also can isolate the interference of upper-layer light rays including strong light, and has the advantages of simple structure, low cost and accurate control.
(iii) The mechanism of the invention adopts a mode that the bottom motor 81 drives the movable light blocking sheet 31, increases the minimum reflection path of light rays in the thickness direction of the movable light blocking sheet 31, greatly improves the light blocking effect, thoroughly blocks the external stray light at the bottom of the reaction cup, and has remarkable effect. Because the movable light blocking sheet 31 is thickened, the number of the light blocking sheets is reduced from four sheets to two sheets, the size of the component is obviously reduced, and the manufacturing cost of the component is reduced.
(iv) Under the condition of serious environment stray light interference, the mechanism only adopts one motor 81, and has the advantages of simple structure, good effect, low cost, few faults and high space utilization rate.
Of course, the present invention is not limited to the above-described embodiments, and those skilled in the art can make various modifications to the above-described embodiments of the present invention without departing from the scope of the present invention under the teaching of the present invention.
Claims (10)
1. A chemiluminescent immunoassay analyzer having a waste liquid extraction port (6) and a cleaning port (7), characterized in that the chemiluminescent immunoassay analyzer comprises a reaction cup (4), an upper shielding member (10) and a side shielding member (20), the upper shielding member (10) and the side shielding member (20) can shield light from reaching the reaction cup (4) from the waste liquid extraction port (6) and the cleaning port (7),
the upper shading part (10) comprises an injection cover plate (11) arranged above the reaction cup (4),
the side shading part (20) comprises a reaction cup turntable (22) which can rotate around the central axis of the side shading part, and a light blocking small cover plate (21) which is arranged between the liquid injection cover plate (11) and the reaction cup turntable (22),
reaction cup carousel (22) are provided with the confession reaction cup (4) pass through's carousel through-hole on axial (A), the little apron (21) that is in the light is the cylindric, the pot head of the little apron (21) that is in the light on axial (A) is located annotate liquid apron (11), the other end and support and lean on reaction cup carousel (22) surrounds the carousel through-hole.
2. The chemiluminescent immunoassay analyzer of claim 1, wherein the end of the liquid injection cover plate (11) facing the reaction cup turntable (22) is formed with two concentric cylindrical structures, and the light blocking small cover plate (21) is embedded between the two cylindrical structures.
3. The chemiluminescent immunoassay analyzer of claim 1, wherein the side shielding member (20) further comprises an upper cover plate (23), the upper cover plate (23) is formed with an inner cavity and an upper opening, the inner cavity of the upper cover plate (23) accommodates the reaction cup rotating disk (22), the upper opening of the upper cover plate (23) is matched with the outer peripheral surface of the light blocking small cover plate (21), and the liquid injection cover plate (11) is fixed on the upper cover plate (23).
4. The chemiluminescent immunoassay analyzer of claim 3 further comprising a lower light shield (30), the lower light shield (30) comprising an incubation tray (31),
the incubation plate (31) accommodates the part of the reaction cups (4) extending out of the reaction cup rotating plate (22),
the inner circumference area of the end face of the incubation disc (31) facing the reaction cup rotating disc (22) partially protrudes towards the reaction cup rotating disc (22) to form a ring-shaped primary step (311), and the primary step (311) extends into the inner cavity of the upper cover plate (23).
5. The chemiluminescent immunoassay analyzer of claim 4, further comprising a photomultiplier tube (5) and a light guide rod (51), the lower light shield (30) further comprising a light guide rod sleeve (33),
one end of the light guide rod (51) penetrates through the side wall of the incubation disc (31) to extend to the reaction cup (4), the other end of the light guide rod (51) extends out of the incubation disc (31) to be connected with the photomultiplier (5),
the light guide rod sleeve (33) wraps the portion of the light guide rod (51) exposed outside the incubation tray (31).
6. The chemiluminescent immunoassay analyzer of claim 4, wherein the lower light shield (30) further comprises two moving light barriers (32),
the incubation plate (31) comprises an incubation well in the shape of a ring groove for receiving a part of the reaction cups (4),
the movable light barrier (32) can move along the axial direction (A) to enter or leave the incubation groove, and when the movable light barrier (32) enters the incubation groove, the two movable light barriers (32) respectively shield light on two sides of the circumference of the reaction cup (4).
7. The chemiluminescent immunoassay analyzer of claim 6, wherein the thickness of the moving light barrier (32) is 3mm to 5 mm.
8. The chemiluminescent immunoassay analyzer of claim 6, characterized in that the dimension of the moving flag is greater than the dimension of the incubation well in the radial direction (R) of the incubation disk (31), the incubation disk (31) being formed with a through-going well in the axial direction (A) to accommodate the moving flag (32).
9. The chemiluminescent immunoassay analyzer of claim 6, wherein the moving flag (32) is driven by a driving mechanism (80), the driving mechanism (80) comprises a lead screw motor (81), a lead screw (82) and a linear guide (83), the lead screw motor (81) provides a driving force, and the linear guide (83) provides a guide for the moving flag (32).
10. The chemiluminescence immunoassay analyzer according to claim 1, wherein the upper shading part (10) further comprises a water pipe connector (12) and a liquid injection water pipe (13), the water pipe connector (12) is connected with the upper part of the liquid injection cover plate (11), and the liquid injection water pipe (13) sequentially passes through the water pipe connector (12) and the liquid injection cover plate (11) and extends to the upper part of the reaction cup (4).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110280696.8A CN112881368A (en) | 2021-03-16 | 2021-03-16 | Chemiluminescence immunity analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110280696.8A CN112881368A (en) | 2021-03-16 | 2021-03-16 | Chemiluminescence immunity analyzer |
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| Publication Number | Publication Date |
|---|---|
| CN112881368A true CN112881368A (en) | 2021-06-01 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110280696.8A Pending CN112881368A (en) | 2021-03-16 | 2021-03-16 | Chemiluminescence immunity analyzer |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115327096A (en) * | 2022-07-20 | 2022-11-11 | 世纪一束(杭州)医学诊断科技有限公司 | Detection disc applied to immunoassay analyzer |
-
2021
- 2021-03-16 CN CN202110280696.8A patent/CN112881368A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115327096A (en) * | 2022-07-20 | 2022-11-11 | 世纪一束(杭州)医学诊断科技有限公司 | Detection disc applied to immunoassay analyzer |
| CN115327096B (en) * | 2022-07-20 | 2023-12-08 | 世纪一束(杭州)医学诊断科技有限公司 | Detection disc applied to immunity analyzer |
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