CN109511771A - A kind of method that biomimetic method prepares functional soy albumen - Google Patents
A kind of method that biomimetic method prepares functional soy albumen Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
- A23J1/001—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste
- A23J1/005—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from waste materials, e.g. kitchen waste from vegetable waste materials
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
- A23J3/16—Vegetable proteins from soybean
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
- A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
- A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
- A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The present invention relates to a kind of methods of the functional feeding soybean protein of biomimetic method preparation, belong to dregs of beans deep process technology field.This method includes: to add water to modulate dregs of beans precomminution, after carrying out primary enzymolysis with non-animal source complex enzyme A, add complex enzyme B carry out secondary enzymolysis, be made secondary enzymolysis dregs of beans slurries, then successively homogeneous, spray drying, sterilizing to get.Present invention process process is simple, it is at low cost, prepared product anti-nutritional factors is all degraded, free amino acid (<186Da) content is high, external digestion absorptivity>=97% or more, high-quality animal-based protein can be effectively substituted, the growth of young animal is remarkably promoted, there is good social and economic benefit.
Description
Technical field
The present invention relates to a kind of methods that biomimetic method prepares functional soy albumen, belong to dregs of beans deep process technology field.
Background technique
Dregs of beans is a kind of good plant protein resource, and proportion is up in feeding all vegetalitas grouts
62%, become most important plant protein fodder raw material.But due to the complicated fine and close stereochemical structure of dregs of beans and pancreas therein
Protease inhibitor, antigen protein, causes the presence of the anti-nutritional factors such as allergenic factor to limit it at non-starch polysaccharide (NSP)
It maximally utilizes.Anti-nutritional factors can destroy or the digestibility and utilization of impeding nutritious substance, generates to animal health and growth performance
Adverse effect.It is a large amount of studies have shown that the problems such as trophism diarrhea, hypoimmunity of young animal, mostly has with the characteristic of dregs of beans
Substantial connection, the antigen in Weaned Growing Pig Diets cause enteron aisle of short duration allergic reaction be Post Weaning Diarrhea determinant,
Antigenic substance present in soybean can cause intestine of young pigs allergy and damage, and then cause diarrhea.Directly contribute protein utilization
Rate is low, wastage of material, the generation for the problems such as environmental pollution and aquaculture cost increase.
Correlative study is it has proven convenient that cause the anaphylactoid major antigen of weanling pig to be glycinin and β-companion's soybean ball
Albumen.It is pushed now with the policy of the in short supply and low-protein diet in dregs of beans market, needs to take necessary means to dregs of beans
Deep processing is carried out, first is that the anti-nutritional factors in dregs of beans is solved, second is that raising dregs of beans albumen digests and absorbs rate.Animal is adopted
The protein of food is degraded to polypeptide first in stomach, by the effect of pepsin etc., and trypsase is then passed through in enteron aisle
With the further enzymatic hydrolysis such as peptase, dregs of beans albumen is further degraded to small peptide and free amino acid, is directly absorbed and utilized by animal.
Animal and bird intestines, especially young age animal and bird intestines can be caused to damage during digestion and absorption not by the dregs of beans of bio-conversion processes
Wound.It is mentioned in CN1292074C and CN1279835C disclosure and prepares low anti-nutritional factors, efficient rate using fermentation method
Dregs of beans, but due to the disadvantage that technique is difficult to control, the unstability for causing fermented bean dregs quality and antigen protein degradation are not
Thoroughly etc.；There is also the problems that similar the said goods quality is difficult to control by CN106417900A；CN106538867A by using
Enzymatic isolation method preparation, technical process controllability is strong, and product is relatively unambiguous, but enzyme class used is less when its primary enzymolysis, institute
It is to decompose non-starch polysaccharide with enzyme main purpose, is not therefore its products made thereby broken wall for the purpose of destroying protein higher structure
Rate is lower, and albumen higher structure is not sufficiently destroyed；Neutral proteinase is only used when secondary enzymolysis, restriction enzyme site is too single, at this time
Product bitterness value is very big, and for quality based on polypeptide, antigen protein fails fully degraded, and digestibility fails sufficiently to maximize.
It can be seen that the prior art is further improved.
Summary of the invention
In view of this, it is necessary to for above-mentioned problem, a kind of method that biomimetic method prepares functional soy albumen is provided,
The method of the present invention thoroughly eliminates the anti-nutritional factors of functional soy albumen, free amino acid (< 186Da, accounting 55% with
On) content height, rate height is digested and absorbed, can effectively be substituted animal protein raw material (fish meal, plasma powder), piglet etc. is promoted
The growth of young animal reduces disease incidence, saves production cost.
To achieve the goals above, the following technical solution is employed by the present invention:
The method that biomimetic method of the present invention prepares functional soy albumen, comprising: by dregs of beans precomminution, water is added to modulate, use is non-
After animal derived complex enzyme A carries out primary enzymolysis, adds complex enzyme B and carries out secondary enzymolysis, secondary enzymolysis dregs of beans slurries are made,
Then successively homogeneous, spray drying, sterilizing to get.
Further, the dregs of beans raw material is low-temperature defatted dehulled soybean meal, and the partial size of precomminution crosses 40~80 meshes.
The dregs of beans that the preferred crude protein content of the present invention is 46%.
Further, the ratio of the additive amount of the water and Soybean Meal is 3~10:1.It, can be by beans under this additive amount
Dregs of rice raw material is evenly dispersed, and can promote the progress digested in next step.
Further, primary enzymolysis process be under conditions of pH=3~6,45~60 DEG C, digest 3~10h, enzyme deactivation, i.e.,
Obtain a dregs of beans enzymolysis liquid.Primary enzymolysis can effectively destroy the higher structure of dregs of beans albumen, expose protein ingredient sufficiently, be
Secondary enzymolysis is prepared.
Preferably, the enzyme deactivation condition of primary enzymolysis process is, enzymolysis liquid is warming up to 85~95 DEG C, enzyme deactivation 10~
30min。
Further, the secondary enzymolysis is to digest 2~10h at a temperature of 50~60 DEG C and digest to get to secondary dregs of beans
Liquid.
Preferably, dregs of beans enzymolysis liquid pH value of gained is adjusted to 6~10, adds complex enzyme B after primary enzymolysis
Carry out secondary enzymolysis.
Further, the complex enzyme A is oxyphilous aspergillus niger and the mixed enzyme that aspergillus oryzae fermentation is extracted；
The additive amount of the complex enzyme A is the 1%~5% of dregs of beans solid quality.The complex enzyme A that the present invention adds can have
Effect destroys the higher structure of dregs of beans albumen, exposes protein ingredient sufficiently.
As some of which embodiment of the present invention, the complex enzyme A extracts fermentation process are as follows: by the black song after activation
Mould and aspergillus oryzae strain is seeded in mould fluid nutrient medium with 2~5:1 according to 2~8% additive amount, and culture 10~for 24 hours,
Gained culture solution low temperature (at a temperature of 37~50 DEG C) is concentrated in vacuo to the 1/2 of original volume.
As some of which embodiment of the present invention, the complex enzyme A enzyme activity be respectively as follows: cellulase 55000~
62000U/ml, 48000~53000U/ml of hemicellulase, 29000~32000U/ml of pectase, zytase 9800~
11000U/ml, 29000~33000U/ml of laccase, 97800~120000U/ml of phytase.
As some of which embodiment of the present invention, the mould fluid nutrient medium the preparation method comprises the following steps: in every liter of water
5g peptone, 10g glucose, 1g potassium dihydrogen phosphate, 0.5g magnesium sulfate and 0.1g chloramphenicol are added, is heated with stirring to completely molten
Solution, adjustment pH are 3~6,115 DEG C of sterilizing 20min, and cooling is spare.
Further, the complex enzyme B is neutrophilic hay bacillus and the compound protease that aspergillus oryzae fermentation is extracted；
The additive amount of the complex enzyme B is the 1.5%~10% of dregs of beans solid quality.Complex enzyme B is by dregs of beans albumen water
Solution is at free amino acid (< 186Da, 55% or more accounting) and a small amount of small peptide (between 186~1000Da, accounting 8~15%).
As some of which embodiment of the present invention, the extraction fermentation process of the complex enzyme B are as follows: will be withered after activation
Straw bacterium and aspergillus oryzae strain are seeded in comprehensive potato fluid nutrient medium with 2~6:1 according to 2~10% additive amount, training
Feeding 18~for 24 hours, gained culture solution low temperature (at a temperature of 30~50 DEG C) is concentrated in vacuo to the 1/3 of original volume.
As some of which embodiment of the present invention, the enzyme activity of the complex enzyme B is 10000~50000U/ml.
As some of which embodiment of the present invention, it is described make comprehensive potato fluid nutrient medium the preparation method comprises the following steps:
200g peeled potatoes are cut into small pieces, water 1000mL is added, 30 minutes then filtered through gauze is boiled, supplies dehydration to 1000ml body
Product.It states then up and 3g potassium dihydrogen phosphate, 1.5g magnesium sulfate, 20g glucose, 10mg dimension life is added in 1000mL potato leaching juice
Element dissolves by heating, and adjustment pH is 7~10,115 DEG C of sterilizing 20min, and cooling is spare.
By above-mentioned secondary dregs of beans enzymolysis liquid, through homogeneous, hig h-speed centrifugal spray drying to get functional to high-quality feed
Soybean protein.
Further, the homogenizer is high speed homogenization mulser.
Further, the high speed homogenization mulser, speed are 5000~8000 turns/min, 30~90min of homogenizing time.
Further, the inlet air temperature of the spray drying is 180~220 DEG C, and air outlet temperature is 60~80 DEG C.
The present invention discharges after completing spray drying, is sieved, products obtained therefrom 80% or above by 80 meshes.
Beneficial effects of the present invention are as follows:
1, method of the invention extracts to obtain efficient composite enzyme A and complex enzyme B using microorganism, is segmented enzyme using biomimetic method
Solution, by dregs of beans proteolysis at optimal state, hydrolysis efficiency is high.
2, the dregs of beans protein product crude protein content prepared by the present invention is 56% or more, trypsin ihhibitor, widow
The anti-nutritional factors such as sugar and Soybean antigen protein are degradable, wherein relative molecular weight < 1000Da (relative molecular weight in albumen
It is 55% or more between 0~186Da, is 3.5~6% between 186~800Da, is 3 between 800~1000Da
~10%) content is up to 65% or more, and in vitro digestion rate is up to 97% or more, and absorption easy to digest can be replaced effectively
For animal protein raw material (fish meal, plasma powder), pigling immunity and premunition are significantly increased, the life of young animal is effectively facilitated
It is long.
3, preparation method process flow of the invention is simple, raw material sources are extensive, production cost is low, is suitble to industrialized push away
Extensively, there is good social and economic benefit.
Specific embodiment
To make the object, technical solutions and advantages of the present invention clearer, below in conjunction with the embodiment of the present invention, to this hair
Bright technical solution work further clearly and completely describes.It should be noted that described embodiment is only the present invention one
Section Example, instead of all the embodiments.Based on the embodiments of the present invention, those of ordinary skill in the art are not doing
Every other embodiment obtained under the premise of creative work out, shall fall within the protection scope of the present invention.
Embodiment 1
Extracting degreasing dehulled soybean meal raw material 500kg, crosses 40 meshes for its precomminution, adds water (dregs of beans: water=1:5) stirring equal
It is even, it is configured to the dregs of beans mixed liquor that solid content is 10~30%；The pH value of dregs of beans mixed liquor is adjusted to 3.5, complex enzyme A is added
(the 3.5% of dregs of beans solid quality), digests 6h at 50 °C, and enzymolysis liquid is warming up to 85 DEG C of enzyme deactivation 20min to get arriving
Dregs of beans enzymolysis liquid；The pH value of dregs of beans enzymolysis liquid is adjusted to 8, is added complex enzyme B (the 8% of dregs of beans solid quality),
7h is digested at a temperature of 55 DEG C to get secondary dregs of beans enzymolysis liquid is arrived；After obtained secondary dregs of beans enzymolysis liquid homogeneous 30 minutes,
It is 180 DEG C in inlet air temperature, under the conditions of air outlet temperature is 80 DEG C, carries out hig h-speed centrifugal spray drying to get high-quality function is arrived
It can property dregs of beans albumen.
The present embodiment, stable product quality contain a large amount of free amino acids (< 186Da, accounting 55%) and a small amount of small peptide
(it is 7% between 186~800Da, is 3%) between 800~1000Da.
Embodiment 2
The present embodiment is unlike above-described embodiment 1: 1) taking dehulled soybean meal raw material 300kg, dregs of beans is with water weight ratio
The amount of 1:6, the complex enzyme A being added are 2%, are 4.5 in pH, under the conditions of temperature is 40 DEG C, digest 7h；2) high temperature enzyme deactivation:
After complex enzyme A enzymatic hydrolysis, 80 DEG C are warming up to, is maintained 30 minutes；3) amount for the complex enzyme B being added is 3%, is 7 in pH, temperature
Under the conditions of 55 DEG C, 6h is digested；It 4) is 200 DEG C in inlet air temperature by after obtained secondary dregs of beans enzymolysis liquid homogeneous 30 minutes,
Under the conditions of air outlet temperature is 70 DEG C, hig h-speed centrifugal spray drying is carried out.
The present embodiment, stable product quality contain a large amount of free amino acids (< 186Da, accounting 57%) and a small amount of small peptide
(it is 5% between 186~800Da, is 8%) between 800~1000Da.
Embodiment 3
The present embodiment is unlike above-described embodiment 1: 1) taking dehulled soybean meal raw material 150kg, dregs of beans is with water weight ratio
The amount of 1:7, the complex enzyme A being added are 5%, are 5.5 in pH, under the conditions of temperature is 45 DEG C, digest 6.5h；2) high temperature enzyme deactivation:
After complex enzyme A enzymatic hydrolysis, 90 DEG C are warming up to, is maintained 10 minutes；3) amount for the complex enzyme B being added is 7%, is 9 in pH, temperature
Under the conditions of degree is 50 DEG C, 3.5h is digested；4) by after obtained secondary dregs of beans enzymolysis liquid homogeneous 30 minutes, it is in inlet air temperature
230 DEG C, under the conditions of air outlet temperature is 50 DEG C, carry out hig h-speed centrifugal spray drying.
The present embodiment, stable product quality, trip contain a large amount of isolated amino acids (< 186Da, accounting 58%) and a small amount of small peptide
(it is 4% between 186~800Da, is 10%) between 800~1000Da.
One, it is the technical effect for further verifying the present invention program, the present invention is tested as follows:
1.1 bitterness value indexs
1 bitterness value index of table
| Group | Bitterness value |
| Product prepared by the embodiment of the present application 1 | 0~1 |
| Product prepared by the embodiment of the present application 2 | 0 |
| Product prepared by the embodiment of the present application 3 | 0~1 |
| According to CN106538867A patent products made thereby (embodiment 1) | 4 |
| According to CN102178205A patent products made thereby (embodiment 1) | 5 |
| According to CN106417900A patent products made thereby (embodiment 2) | 4 |
| Dregs of beans is as former state | ------ |
By upper table 1 it is found that the functional soy albumen that the application method obtains, degradation rate is high, bitterness value is substantially reduced,
Improve the palatability of feeding.
Note: quinine is configured to the solution of various concentration, is scored as standard.The concentration of standard solution is respectively
0、8×10-6、1.6×10-5、2.4×10-5、3.2×10-5With 4 × 10-5G/mL, corresponding score value are 0,1,2,3,4 and 5 point,
Bitter taste is heavier, and score is higher.Sample (concentration 1% (w/v), pH=6.5) is compared at room temperature with standard solution, and is pressed
It scores according to standard score sample to be tested, shares 20 sensory evaluation persons and participate in evaluation, wherein 10 people of male, women 10
People.
1.2 anti-nutritional factors indexs
2 anti-nutritional factors index of table
By upper table 2 it is found that the functional soy albumen that the application method obtains, raffinose, stachyose, glycinin
And beta-conglycinin content's index is in reduced levels.
1.3 peptide relative molecular weight distributions
3 peptide relative molecular weight distribution index of table
By upper table 3 it is found that the relative molecular weight distribution ﹤ 186Da for the functional soy albumen that the application method obtains is accounted for
55% or more, the anti-nutritional factors such as trypsin ihhibitor, oligosaccharides and Soybean antigen protein are degradable.
Two, it is the technical effect for further verifying the present invention program, laboratory simulation digestion trial effect is as follows:
2.1 simulation Pepsin digestibility test indexs
Table 4 simulates Pepsin digestibility
As shown in Table 4, in vitro digestion rate is up to 97% or more, product absorption easy to digest prepared by the present invention,
Animal protein raw material (fish meal, plasma powder) can effectively be substituted.
Note: vitro digestion technology is to be disappeared by simulating animal digestion physilogical characteristics using with similar in animal body
Change environment and Digest enzyme, evaluates a kind of method that feed digestion absorbs in vitro and (refer to Zhang Tieying etc., 2005 < nonruminant
Vitro digestion technology progress >).
Three, it is the technical effect for further verifying the present invention program, animal applications test effect is as follows:
3.1 MATERIALS METHODS
3.1.1 experimental design and grouping
5 experimental design of table
| Group | Addition scheme |
| Handle 1 group | Basal diet group |
| Handle 2 groups | The albumen such as product prepared by the embodiment of the present invention 1 substitute fish meal group |
| Handle 3 groups | By the preparation in 106538867 A embodiment 1 of CN, albumen is waited to substitute fish meal group |
| Handle 4 groups | By the preparation in 106417900 A embodiment 2 of CN, albumen is waited to substitute fish meal group |
The weanling pig 192 (about 8.5kg/) that age in days is consistent, healthy is selected, 4 processing, every processing 4 are randomly divided into
A repetition, 12 piglets of every repetition.By the corresponding test daily ration of every group of experimental design (being shown in Table 5), the period is 21 days, test
Forward and backward every group of weighing.
3.1.2 daily ration is tested
6 basal diet of table
3.1.3 feeding management
Test pig house daily management is executed by pig farm normal procedure during test.Take the feeding of free choice feeding, free water
The method of feeding.Pig house sanitation and hygiene are kept, ventilation condition is good.Piglet diseases, death are observed and are recorded in timing daily during test
Situations such as.
3.1.4 measurement item
3.1.4.1 growth performance
Record feed intake, extremely naughty pig quantity and weight daily calculate each stage average daily gain, day consumption during test
Material, feedstuff-meat ratio and death and culling rate.
Average daily gain=(addition feed total amount-hopper remaining material)/(the column residue head number × total number of days of test+dead naughty
Pig's head number × test number of days)
Average daily gain=(column end weight-column initial weight+extremely naughty pig end weight)/(the column residue head number × total number of days of test+dead naughty
Pig's head number × test number of days)
Feedstuff-meat ratio=average daily feed intake/Average weight increasing a day
Death and culling rate=(dead to wash in a pan sum × 100%)/every group of piglet sum；
3.1.4.2 diarrhea rate
Record grice diarrhoea situation daily.
The total head of diarrhea rate=diarrhea × 100%/(testing total head number × total number of days of test).
3.1.4.3 appearance scores
Observe weekly it is relatively primary, according to the hair of pig, color, can be divided into it is poor, in, good, excellent 4 grades (scoring respectively 1,
2,3,4 points)；
3.1.5 data statistics and analysis
Test data is arranged using Excel 2010, carries out the one-factor analysis of variance with SPSS16.0 software, and adopt
Significance test of difference is carried out with Dun-can method, is as a result indicated with average ± standard error.
3.2, result and analysis
3.2.1 experimental result
Influence of the 7 different process protein sources of table to suckling piglet production performance
As shown in Table 7, in piglet diet, the average end weight of 2 suckling piglet of processing group, average feed intake, average daily gain are bright
It is aobvious to be higher than 4 groups of processing 1, processing 3 and processing；From feedstuff-meat ratio, 2 suckling piglet feedstuff-meat ratio of processing group is better than control treatment 1 and place
Manage 4 groups；From diarrhea rate, 2 suckling piglet diarrhea rate of processing group is minimum；The hair color appearance of each processing group suckling piglet is without significance difference
It is different.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to guarantor of the invention
Protect range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (9)
1. a kind of method that biomimetic method prepares functional soy albumen characterized by comprising by dregs of beans precomminution, add water tune
System after carrying out primary enzymolysis with non-animal source complex enzyme A, adds complex enzyme B and carries out secondary enzymolysis, secondary enzymolysis beans are made
Dregs of rice slurries, then successively homogeneous, spray drying, sterilizing to get.
2. the method that biomimetic method according to claim 1 prepares functional soy albumen, which is characterized in that the dregs of beans is former
Material is low-temperature defatted dehulled soybean meal, and the partial size of precomminution crosses 40~80 meshes.
3. the method according to claim 1, wherein the water additive amount and Soybean Meal ratio be 3~
10:1。
4. the method that biomimetic method according to claim 1 prepares functional soy albumen, which is characterized in that primary enzymolysis mistake
Journey is to digest 3~10h, sterilizing is to get to a dregs of beans enzymolysis liquid under conditions of pH=3~6,45~60 DEG C.
5. the method that biomimetic method according to claim 4 prepares functional soy albumen, which is characterized in that primary enzymolysis mistake
The enzyme deactivation condition of journey is enzymolysis liquid to be warming up to 85~95 DEG C, 10~30min of enzyme deactivation.
6. the method that biomimetic method according to claim 1 or 4 prepares functional soy albumen, which is characterized in that described two
Secondary enzymatic hydrolysis is to digest 2~10h at a temperature of 50~60 DEG C to get secondary dregs of beans enzymolysis liquid is arrived.
7. the method that biomimetic method according to claim 6 prepares functional soy albumen, which is characterized in that primary enzymolysis
Afterwards, dregs of beans enzymolysis liquid pH value of gained is adjusted to 6~10, adds complex enzyme B and carries out secondary enzymolysis.
8. the method that biomimetic method according to claim 1 prepares functional soy albumen, which is characterized in that the complex enzyme
A is oxyphilous aspergillus niger and the mixed enzyme that aspergillus oryzae fermentation is extracted；
The additive amount of the complex enzyme A is the 1%~5% of dregs of beans solid quality.
9. the method that biomimetic method according to claim 1 prepares functional soy albumen, which is characterized in that the complex enzyme
B is neutrophilic hay bacillus and the compound protease that aspergillus oryzae fermentation is extracted；
The additive amount of the complex enzyme B is the 1.5%~10% of dregs of beans solid quality.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111011576A (en) * | 2019-12-30 | 2020-04-17 | 杭州衡美食品科技有限公司 | Preparation method and application of high-dispersion soybean protein |
| CN114343158A (en) * | 2021-12-31 | 2022-04-15 | 好记食品酿造股份有限公司 | Preparation method of plant-based flavor seasoning organic vegetable shrimp sauce |
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| CN102115774A (en) * | 2010-11-30 | 2011-07-06 | 华东理工大学 | Method for preparing plant polypeptide by enzyme process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111011576A (en) * | 2019-12-30 | 2020-04-17 | 杭州衡美食品科技有限公司 | Preparation method and application of high-dispersion soybean protein |
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