CN109645215A

CN109645215A - A kind of mulberry leaf biological feedstuff and its preparation method and application

Info

Publication number: CN109645215A
Application number: CN201910113348.4A
Authority: CN
Inventors: 易显凤; 庞天德; 林波; 姚娜; 王崇洲; 易显菊; 黄龙; 黄一龙
Original assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Current assignee: Guangxi Zhuang Autonomous Region Institute of Animal Husbandry
Priority date: 2019-02-13
Filing date: 2019-02-13
Publication date: 2019-04-19

Abstract

The present invention relates to feed processing technologies, in particular to a kind of mulberry leaf biological feedstuff and its preparation method and application, biological feedstuff is by as follows at being grouped as: fermentation mulberry leaf, extract of mulberry twig, black rice, corn stover, pueraria lobata, sucrose, fermentation cassava slag, fermentation molasses and fermented maize palpus；The application feed formula ferments the ingredients such as mulberry leaf, manioc waste, molasses, corn stigma, herbage ingredient can effectively be softened, the feed formula of the application, which mutually compounds, simultaneously can effectively improve feed mouthfeel, improve feed nutritive value, simultaneously, Flavonoid substances also rich in the application, with animal meat is improved, keep animal meat fresher and tenderer, improves the purpose of porcine protein content.

Description

A kind of mulberry leaf biological feedstuff and its preparation method and application

[technical field]

The present invention relates to feed processing technologies, and in particular to a kind of mulberry leaf biological feedstuff and preparation method thereof and answers With.

[background technique]

Mulberry leaf derive from the leaf of moraceae plants mulberry (Morus alba L.), this platymiscium is that one kind is suitable in warmly band The plant of plantation.Mulberry tree is distributed widely in all parts of the country, mulberry leaf resource very abundant.Protein rich in, carbon in mulberry leaf Hydrate, vitamin, mineral element and natural active matter etc., protein of folium mori are a kind of excellent protein resources.Mulberry leaf As the unconventional water resources of animal, there is great potentiality to be exploited and utility value, in recent years, mulberry leaf are in chicken, pig, ruminant Application in diet it has been reported that but be confined to grind by adjusting mulberry leaf pitch-based sphere with the speed of growth etc. for improving livestock and poultry mostly Study carefully direction.Natural active matter in mulberry leaf mainly has the substances such as flavone compound, polysaccharide and superoxide dismutase.Flavones Class compound has the function of anti-oxidant and reduces cholesterol, and polysaccharide has the effect of reducing blood glucose sugared content, and SOD then can It removes free radical and prevents the effect of aging.If mulberry leaf directly applied in feeding animal, bioavailability cannot be obtained Utilize to fine, and active constituent therein: for example general flavone can not play a significant role, therefore, in order to improve to mulberry The bioavailability of leaf improves the nutritive value of mulberry leaf feed, it is necessary to it is improved for mulberry leaf biological feedstuff processing method, A kind of biological feedstuff with high nutritive value is produced, improves animal meat, keeps animal meat fresher and tenderer, improve the egg of pork White matter content.

[summary of the invention]

In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of mulberry leaf biological feedstuffs and preparation method thereof And application, a kind of biological feedstuff with high nutritive value is produced, improves animal meat, keeps animal meat fresher and tenderer, is improved The protein content of pork.

In order to solve the above technical problems, The technical solution adopted by the invention is as follows:

A kind of mulberry leaf biological feedstuff, the feed are made of following parts by weight ingredient: the feed by following parts by weight at It is grouped as: 97 parts -135 parts of fermentation mulberry leaf, 26 parts -46 parts of extract of mulberry twig, 21 parts -34 parts of black rice, 13 parts -34 parts Corn stover, 31 parts -45 parts of pueraria lobata, 7 parts -18 parts of sucrose, 32 parts -46 parts of fermentation cassava slag, 14 parts -26 parts of fermentation Molasses and 17 parts -26 parts of fermented maize palpus.

Further, the extract of mulberry twig the preparation method comprises the following steps: by moisture content be 3%-5% belt leather ramulus mori carry out powder It is broken, then screened by the sieve of -150 mesh of 100 mesh；Then 40 DEG C -50 DEG C of warm water is added to be impregnated, when immersion is a length of 2h-3h；It is put into after immersion in -20 DEG C~-10 DEG C of refrigerator-freezer and freezes 3h-4h；It thaws, filter under room temperature after freezing, take filter residue equal It is divided into two parts, a copy of it is mixed with the ethanol solution that percentage by volume is 60%-70%, and ultrasound is carried out in supersonic extractors It is filtered after extraction, filtrate is taken to carry out rotary evaporation concentration, until the 1/10-1/8 that concentrate is stoste obtains extract I；It is another Part mixes with the butanol solution that percentage by volume is 10%-20%, and mistake after refluxing extraction 10h-12h is carried out in refluxing extraction device Filter takes filtrate to carry out rotary evaporation concentration, until the 1/20-1/15 that concentrate is stoste obtains extract II；By extract I and Extract II is mixed to get extract of mulberry twig.

Further, the power of the ultrasonic extraction is 150w-200w；Extraction time is 10min-20min.

Further, the Extracting temperature of the refluxing extraction device is 130 DEG C -150 DEG C.

Further, the fermentation process of the fermentation mulberry leaf are as follows: be according to mass ratio with complex enzyme I and water by mulberry leaf cutting 50-60:1:15-20 is accessed according to the inoculum concentration of 2%-5% after mixing and is activated probiotics, the ventilation for being 35 DEG C -38 DEG C in temperature Under the conditions of ferment 4d-6d；The carry out stack retting under confined conditions for being 36-38 DEG C in temperature after ventilating fermentation；A length of 5d- when stack retting 7d obtains fermentation mulberry leaf；The carboxypeptidase and enzyme-activity unit that the complex enzyme I is 300U/mg-800U/mg by enzyme-activity unit be 500U/mg-700U/mg cellulase is 1-3:2-4 composition according to mass ratio.

Further, the fermentation process of the fermented maize palpus are as follows: it will be cooled to room temperature after corn stigma blanching, it is then and multiple Synthase II is 50-60:1-3 mixing, mixed enzymolysis 12h-14h, then to the corn stigma after enzymatic hydrolysis according to 1%- according to mass ratio 4% inoculum concentration access activation probiotics is 36-38 DEG C of carry out stack retting under confined conditions in temperature, when stack retting a length of 5d- 7d；Ventilating fermentation 4d-6d obtains fermented maize palpus under conditions of temperature is 33 DEG C -37 DEG C after stack retting；The compound enzyme II by Carboxypeptidase that enzyme-activity unit is 300U/mg-800U/mg, enzyme-activity unit are 500U/mg-700U/mg cellulase and enzyme-activity unit For 1000U/mg-1500U/mg pectase according to mass ratio be 1-4:5-7:2-5 composition.

Further, the fermentation cassava slag the preparation method comprises the following steps: being to COD content by the inoculum concentration of 1%-3% Access activation probiotics in the manioc waste of 500mg/L-900mg/L is 0.1vvm-1.5vvm in the ventilatory capacity of air, and temperature is Aerobic fermentation 20h-24h under the conditions of 35 DEG C -38 DEG C；After aerobic fermentation temperature be 37 DEG C under the conditions of anaerobic fermentation 2d-4d, To obtain the final product.

Further, it is described fermentation molasses the preparation method comprises the following steps: by 1%-3% inoculum concentration to COD content be 1000mg/ Access activation probiotics in the molasses of L-1500mg/L is 0.5vvm-1.8vvm in the ventilatory capacity of air, and temperature is 35 DEG C -38 Aerobic fermentation 20h-24h under the conditions of DEG C；After aerobic fermentation temperature be 37 DEG C under the conditions of anaerobic fermentation 3d-5d to get.

Further, the activation probiotics is 1.5 × 10 by living bacteria count⁸-4.0×10⁸The lactic acid bacteria of cfu/mg and Living bacteria count is 2.5 × 10⁸-5.0×10⁸The saccharomycete of cfu/mg is mixed to prepare according to mass ratio for 2-4:3-6；The cream The activation method of sour bacterium are as follows: in the fluid nutrient medium of lactic acid bacteria under anaerobic by lactobacillus inoculum, revolving speed 500r/ Min-800r/min, temperature are shaking table culture 48-52h under the conditions of 37 DEG C, and the fluid nutrient medium of the lactic acid bacteria is by following ingredient Composition: yeast powder 10-15g/L, beef extract 10-15g/L, sodium citrate 2-5g/L, sodium chloride 2-4g/L, gumbo polysaccharide 10- 15g/L, auxiliary agent 100mL-150mL, manioc waste leaching liquor 20-30g/L, molasses leaching liquor 5-10g/L, the above ingredient dissolution are used Sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min；The activation method of the saccharomycete are as follows: saccharomycete is inoculated in saccharomycete Fluid nutrient medium under anaerobic, revolving speed 500r/min-800r/min, temperature be 37 DEG C under the conditions of shaking table culture 48-52h, the fluid nutrient medium of the saccharomycete is by as follows at being grouped as: yeast powder 7-12g/L, beef extract 8-16g/L, carbonic acid Hydrogen sodium 3-6g/L, sodium chloride 2-4g/L, aloe polysaccharide 10-15g/L, auxiliary agent 100mL-150mL, manioc waste leaching liquor 30-40g/ L, molasses leaching liquor 10-15g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizing 20min.

Further, the auxiliary agent the preparation method comprises the following steps: be according to mass ratio by Radix Isatidis, pawpaw skin, banana and Eucalyptus bits 1:1-3:4-7:2-4 mixing, the sucrose for being then 10g/L-15g/L with concentration is that 2-4:1-3 is mixed according to mass ratio, is sealed in 10d-15d in watt cylinder filters to take supernatant to obtain the final product.

Present invention also provides a kind of methods for preparing mulberry leaf biological feedstuff, and described method includes following steps:

(1) each raw material is weighed by the parts by weight；

(2) fermentation mulberry leaf, pueraria lobata, black rice, sucrose, fermentation cassava slag, fermentation molasses and fermented maize must be mixed, 35 Stack retting 2d-3d under the conditions of DEG C -37 DEG C；Then steam sterilizing 40min-50min under the conditions of 120 DEG C -130 DEG C；Then at -4 DEG C 10min is freezed under the conditions of~0 DEG C, obtains the mixture of fermentation material；

(3) corn straw smashing is crossed after the screening of -150 mesh screen of 100 mesh to the fermentation material mixing for being then added to step (2) Object, then sprays after extract of mulberry twig mixes again that constantly water spray until mixture hand is pinched agglomerating is into mixture into mixture Only, under the conditions of 36 DEG C -38 DEG C stack retting 3d-4d, under the conditions of 60 DEG C -70 DEG C drying obtain the biological feedstuff of the application.

Present invention also provides a kind of mulberry leaf biological feedstuffs to improve the purposes in porcine protein content.

Compared with prior art, the invention has the following beneficial effects: the feed formulas of the application and preparation method to drop Cyanide content, raising crude protein content, reduce crude fiber content, improve dry matter, total amino acid and general flavone in low feed Content；It can be effectively improved animal meat using the feed of the application, keep animal meat fresher and tenderer, improve the protein content of pork

1, the mulberry leaf biological feedstuff of the application is by as follows at being grouped as: fermentation mulberry leaf, extract of mulberry twig, black rice, corn stalk Stalk, pueraria lobata, sucrose, fermentation cassava slag, fermentation molasses and fermented maize palpus；Mutually compounding can be mentioned effectively between the application feed formula High feed mouthfeel improves feed nutritive value, can be effectively improved animal meat, keep animal meat fresher and tenderer, improve the egg of pork White matter content, mulberry leaf can soften mulberry leaf after everfermentation, make mulberry leaf be easy to be digested and assimilated by animal, absorptivity is higher, sends out simultaneously The ingredients such as the effective component after ferment in mulberry leaf, such as general flavone, polysaccharide are precipitated, and can effectively improve the nutritive value of feed, can Improve the antioxidant activity of feed, at the same protein of folium mori digested, ferment after favor decomposing into the amino acid of small molecule；This Shen Manioc waste please is to squeeze the waste residue being prepared after cassava carries out full slag anaerobism, aerobic wastewater treatment after alcoholic fermentation, Include multiple-microorganism, microelement；Manioc waste organic matter therein after everfermentation is enriched with, so that albumen contains in feed Measure cyanide content that is richer, while can also being effectively reduced in manioc waste；Feeding can be improved in the molasses extraction post-fermentation of the application Dry matter content in material；The application corn stigma contains abundant flavones and its glycoside substance, effective component can be made to obtain after fermentation Enrichment, meanwhile, the reasonable compounding between each material composition improves feed mouthfeel, so as to improve animal meat, makes animal flesh Matter is fresher and tenderer, improves the protein content of pork.

2, containing abundant Flavonoid substances in the feed of the application, the oxidation resistance of cell is can be improved in Flavonoid substances, With growth of animal is promoted, increase immunity of organisms, reconcile animal reproduction, improves degree Fresh & Tender in Texture to play, improve meat The purpose of protein content.

[specific embodiment]

A specific embodiment of the invention is described further with reference to embodiments.

Embodiment 1

The processing method of the present embodiment mulberry leaf biological feedstuff the following steps are included:

(1) preparation fermentation mulberry leaf: by mulberry leaf cutting and complex enzyme I and water according to mass ratio be after 50:1:15 is mixed according to 2% inoculum concentration access activation probiotics, ferment 4d in the case where temperature is 35 DEG C of ventilation condition；After ventilating fermentation temperature be 36 DEG C carry out stack retting under confined conditions；A length of 5d when stack retting obtains fermentation mulberry leaf；The complex enzyme I is 300U/ by enzyme-activity unit It is 1:2 composition that the carboxypeptidase and enzyme-activity unit of mg, which is 500U/mg cellulase according to mass ratio,.

(2) extract of mulberry twig extracts: the belt leather ramulus mori that moisture content is 3% being crushed, the sieve of 100 mesh is then passed through Screening；Then 40 DEG C of warm water is added to be impregnated, when immersion a length of 2h；It is put into after immersion in -20 DEG C of refrigerator-freezer and freezes 3h；It is cold It thaws, filter under room temperature after jelly, take filter residue to be divided into two parts, a copy of it and percentage by volume are mixed for 60% ethanol solution It closes, is filtered after carrying out ultrasonic extraction 10min in the supersonic extractors that power is 150w, filtrate is taken to carry out rotary evaporation concentration, Until concentrate obtains extract I for the 1/10 of stoste；Another is mixed with the butanol solution that percentage by volume is 10%, is being mentioned It takes and is filtered after carrying out refluxing extraction 10h in the refluxing extraction device that temperature is 130 DEG C, filtrate is taken to carry out rotary evaporation concentration, until Concentrate obtains extract II for the 1/20 of stoste；Extract I and extract II are mixed to get extract of mulberry twig.

(3) prepare fermentation cassava slag: by 1% inoculum concentration, into the manioc waste that COD content is 500mg/L, access activation is beneficial Raw bacterium is 0.1vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 20h under the conditions of 35 DEG C；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 2d to get.

(4) preparation fermentation molasses: by 1% inoculum concentration, into the molasses that COD content is 1000mg/L, access activation is prebiotic Bacterium is 0.5vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 20h under the conditions of 35 DEG C；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 3d to get.

(5) it prepares fermented maize palpus: will be cooled to room temperature after corn stigma blanching, be then according to mass ratio with compound enzyme II Then 50:1 mixing, mixed enzymolysis 12h activate probiotics according to 1% inoculum concentration access to the corn stigma after enzymatic hydrolysis, in temperature For 36 DEG C of carry out stack retting under confined conditions, when stack retting a length of 5d；After stack retting under conditions of temperature is 33 DEG C ventilating fermentation 4d Obtain fermented maize palpus；Carboxypeptidase that the compound enzyme II is 300U/mg by enzyme-activity unit, enzyme-activity unit are 500U/mg fiber The pectase that plain enzyme and enzyme-activity unit are 1000U/mg is 1:5:2 composition according to mass ratio.

(6) each raw material is weighed by by following parts by weight: 97 parts of fermentation mulberry leaf, 26 parts of extract of mulberry twig, 21 parts black Rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of fermentation cassava slag, 14 parts of fermentation molasses and 17 parts Fermented maize palpus.

(7) fermentation mulberry leaf, pueraria lobata, black rice, sucrose, fermentation cassava slag, fermentation molasses and fermented maize must be mixed, 35 Stack retting 2d under the conditions of DEG C；Then steam sterilizing 40min under the conditions of 120 DEG C；Then 10min is freezed under the conditions of -4 DEG C, obtained The mixture of fermentation material.

(8) corn straw smashing is sieved with 100 mesh sieve to the fermentation material mixture that step (7) is then added to after mesh screen selects, then It sprays into mixture after extract of mulberry twig mixes and constantly sprays water until mixture hand pinches agglomerating into mixture again, 36 Stack retting 3d under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 60 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 1.5 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 2.5 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is mixed to prepare according to mass ratio for 2:3.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 500r/min, temperature is shaking table culture 48h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, auxiliary agent 100mL, manioc waste leaching liquor 20g/L, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C Sterilize 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 500r/min, temperature is shaking table culture 48h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, aloe polysaccharide 10g/L, auxiliary agent 100mL, Manioc waste leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C of sterilizings 20min。

Wherein, auxiliary agent mixes the preparation method comprises the following steps: considering to be worth doing Radix Isatidis, pawpaw skin, banana and Eucalyptus according to mass ratio for 1:1:4:2 It closes, the sucrose for being then 10g/L with concentration is that 2:1 is mixed according to mass ratio, is sealed in 10d in watt cylinder, filters to take supernatant i.e. ?.

The manioc waste of the present embodiment is to squeeze system after cassava carries out full slag anaerobism, aerobic wastewater treatment after alcoholic fermentation Waste residue obtained by standby, includes multiple-microorganism, microelement and a small amount of alcohol.Manioc waste leaching liquor the preparation method comprises the following steps: by wooden Potato slag is that 1:2 is mixed according to mass ratio with water, is then placed in -3 DEG C of refrigerator-freezer and freezes 8h；It is filtered to take after normal temperature unfreezing to room temperature Filtrate to obtain the final product.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:1 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Embodiment 2

(1) preparation fermentation mulberry leaf: by mulberry leaf cutting and complex enzyme I and water according to mass ratio be after 60:1:20 is mixed according to 5% inoculum concentration access activation probiotics, ferment 6d in the case where temperature is 38 DEG C of ventilation condition；After ventilating fermentation temperature be 38 DEG C carry out stack retting under confined conditions；A length of 7d when stack retting obtains fermentation mulberry leaf；The complex enzyme I is 800U/ by enzyme-activity unit It is 3:4 composition that the carboxypeptidase and enzyme-activity unit of mg, which is 700U/mg cellulase according to mass ratio,.

(2) extract of mulberry twig extracts: the belt leather ramulus mori that moisture content is 5% being crushed, the sieve of 150 mesh is then passed through Screening；Then 50 DEG C of warm water is added to be impregnated, when immersion a length of 3h；It is put into after immersion in -10 DEG C of refrigerator-freezer and freezes 4h；It is cold It thaws, filter under room temperature after jelly, take filter residue to be divided into two parts, a copy of it and percentage by volume are mixed for 70% ethanol solution It closes, is filtered after carrying out ultrasonic extraction 20min in the supersonic extractors that power is 200w, filtrate is taken to carry out rotary evaporation concentration, Until concentrate obtains extract I for the 1/8 of stoste；Another is mixed with the butanol solution that percentage by volume is 20%, is being mentioned It takes and is filtered after carrying out refluxing extraction 12h in the refluxing extraction device that temperature is 150 DEG C, filtrate is taken to carry out rotary evaporation concentration, until Concentrate obtains extract II for the 1/15 of stoste；Extract I and extract II are mixed to get extract of mulberry twig.

(3) prepare fermentation cassava slag: by 3% inoculum concentration, into the manioc waste that COD content is 900mg/L, access activation is beneficial Raw bacterium, air ventilatory capacity be 1.5vvm, temperature be 38 DEG C under the conditions of aerobic fermentation for 24 hours；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 4d to get.

(4) preparation fermentation molasses: by 3% inoculum concentration, into the molasses that COD content is 1500mg/L, access activation is prebiotic Bacterium, air ventilatory capacity be 1.8vvm, temperature be 38 DEG C under the conditions of aerobic fermentation for 24 hours；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 5d to get.

(5) it prepares fermented maize palpus: will be cooled to room temperature after corn stigma blanching, be then according to mass ratio with compound enzyme II Then 60:3 mixing, mixed enzymolysis 14h activate probiotics according to 4% inoculum concentration access to the corn stigma after enzymatic hydrolysis, in temperature For 38 DEG C of carry out stack retting under confined conditions, when stack retting a length of 7d；After stack retting under conditions of temperature is 37 DEG C ventilating fermentation 6d Obtain fermented maize palpus；Carboxypeptidase that the compound enzyme II is 800U/mg by enzyme-activity unit, enzyme-activity unit are 700U/mg fiber The pectase that plain enzyme and enzyme-activity unit are 1500U/mg is 4:7:5 composition according to mass ratio.

(6) each raw material is weighed by by following parts by weight: 135 parts of fermentation mulberry leaf, 46 parts of extract of mulberry twig, 34 parts black Rice, 34 parts of corn stover, 45 parts of pueraria lobata, 18 parts of sucrose, 46 parts of fermentation cassava slag, 26 parts of fermentation molasses and 26 parts Fermented maize palpus.

(7) fermentation mulberry leaf, pueraria lobata, black rice, sucrose, fermentation cassava slag, fermentation molasses and fermented maize must be mixed, 37 Stack retting 3d under the conditions of DEG C；Then steam sterilizing 50min under the conditions of 130 DEG C；Then 10min is freezed under the conditions of 0 DEG C, is sent out The mixture of ferment material.

(8) corn straw smashing is crossed after the screening of 150 mesh screens to the fermentation material mixture for being then added to step (7), then It sprays into mixture after extract of mulberry twig mixes and constantly sprays water until mixture hand pinches agglomerating into mixture again, 38 Stack retting 4d under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 70 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 4.0 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 5.0 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is mixed to prepare according to mass ratio for 4:6.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 800r/min, temperature is shaking table culture 52h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 15g/L, beef extract 15g/L, sodium citrate 5g/L, sodium chloride 4g/L, gumbo polysaccharide 15g/L, auxiliary agent 150mL, manioc waste leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C sterilizing 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 800r/min, temperature is shaking table culture 52h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 12g/L, beef extract 16g/L, sodium bicarbonate 6g/L, sodium chloride 4g/L, aloe polysaccharide 15g/L, auxiliary agent 150mL, manioc waste leaching liquor 40g/L, molasses leaching liquor 15g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C sterilizing 20min.

Wherein, auxiliary agent mixes the preparation method comprises the following steps: considering to be worth doing Radix Isatidis, pawpaw skin, banana and Eucalyptus according to mass ratio for 1:3:7:4 It closes, the sucrose for being then 15g/L with concentration is that 4:3 is mixed according to mass ratio, is sealed in 15d in watt cylinder, filters to take supernatant i.e. ?.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:3 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Embodiment 3

(1) preparation fermentation mulberry leaf: by mulberry leaf cutting and complex enzyme I and water according to mass ratio be after 55:1:17 is mixed according to 3% inoculum concentration access activation probiotics, ferment 5d in the case where temperature is 37 DEG C of ventilation condition；After ventilating fermentation temperature be 37 DEG C carry out stack retting under confined conditions；A length of 6d when stack retting obtains fermentation mulberry leaf；The complex enzyme I is 500U/ by enzyme-activity unit It is 2:3 composition that the carboxypeptidase and enzyme-activity unit of mg, which is 600U/mg cellulase according to mass ratio,.

(2) extract of mulberry twig extracts: the belt leather ramulus mori that moisture content is 4% being crushed, the sieve of 120 mesh is then passed through Screening；Then 45 DEG C of warm water is added to be impregnated, when immersion a length of 2.5h；It is put into -15 DEG C of refrigerator-freezer and freezes after immersion 3.5h；It thaws, filter under room temperature after freezing, take filter residue to be divided into two parts, the ethyl alcohol that a copy of it is 65% with percentage by volume Solution mixing, is filtered after carrying out ultrasonic extraction 15min in the supersonic extractors that power is 170w, and filtrate is taken to carry out rotary evaporation Concentration, until concentrate obtains extract I for the 1/9 of stoste；Another is mixed with the butanol solution that percentage by volume is 15%, It is filtered after carrying out refluxing extraction 11h in the refluxing extraction device that Extracting temperature is 140 DEG C, filtrate is taken to carry out rotary evaporation concentration, Until concentrate obtains extract II for the 1/18 of stoste；Extract I and extract II are mixed to get extract of mulberry twig.

(3) prepare fermentation cassava slag: by 2% inoculum concentration, into the manioc waste that COD content is 700mg/L, access activation is beneficial Raw bacterium is 0.9vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 22h under the conditions of 37 DEG C；In temperature after aerobic fermentation Under the conditions of being 37 DEG C anaerobic fermentation 3d to get.

(4) preparation fermentation molasses: by 2% inoculum concentration, into the molasses that COD content is 1200mg/L, access activation is prebiotic Bacterium is 0.9vvm in the ventilatory capacity of air, and temperature is aerobic fermentation 22h under the conditions of 37 DEG C；It is in temperature after aerobic fermentation Under the conditions of 37 DEG C anaerobic fermentation 4d to get.

(5) it prepares fermented maize palpus: will be cooled to room temperature after corn stigma blanching, be then according to mass ratio with compound enzyme II Then 55:2 mixing, mixed enzymolysis 13h activate probiotics according to 3% inoculum concentration access to the corn stigma after enzymatic hydrolysis, in temperature For 37 DEG C of carry out stack retting under confined conditions, when stack retting a length of 6d；After stack retting under conditions of temperature is 35 DEG C ventilating fermentation 5d Obtain fermented maize palpus；Carboxypeptidase that the compound enzyme II is 500U/mg by enzyme-activity unit, enzyme-activity unit are 600U/mg fiber The pectase that plain enzyme and enzyme-activity unit are 1200U/mg is 2:6:3 composition according to mass ratio.

(6) each raw material is weighed by by following parts by weight: 112 parts of fermentation mulberry leaf, 36 parts of extract of mulberry twig, 28 parts black Rice, 19 parts of corn stover, 37 parts of pueraria lobata, 13 parts of sucrose, 39 parts of fermentation cassava slag, 19 parts of fermentation molasses and 19 parts Fermented maize palpus.

(7) fermentation mulberry leaf, pueraria lobata, black rice, sucrose, fermentation cassava slag, fermentation molasses and fermented maize must be mixed, 36 Stack retting 2.5d under the conditions of DEG C；Then steam sterilizing 45min under the conditions of 125 DEG C；Then 10min is freezed under the conditions of -3 DEG C, obtained To the mixture of fermentation material.

(8) corn straw smashing is crossed after the screening of 120 mesh screens to the fermentation material mixture for being then added to step (7), then It sprays into mixture after extract of mulberry twig mixes and constantly sprays water until mixture hand pinches agglomerating into mixture again, 37 Stack retting 3.5d under the conditions of DEG C, drying obtains the biological feedstuff of the application under the conditions of 65 DEG C.

Wherein, the activation probiotics of step (1) (3) (4) (5) is 2.0 × 10 by living bacteria count⁸The lactic acid bacteria of cfu/mg It is 4.0 × 10 with living bacteria count⁸The saccharomycete of cfu/mg is mixed to prepare according to mass ratio for 3:5.

Wherein, the activation method of lactic acid bacteria are as follows: by lactobacillus inoculum in anaerobic condition in the fluid nutrient medium of lactic acid bacteria Under, revolving speed 600r/min, temperature is shaking table culture 50h under the conditions of 37 DEG C, the fluid nutrient medium of the lactic acid bacteria by as follows at It is grouped as: yeast powder 12g/L, beef extract 13g/L, sodium citrate 3g/L, sodium chloride 3g/L, gumbo polysaccharide 12g/L, auxiliary agent 120mL, manioc waste leaching liquor 25g/L, molasses leaching liquor 7g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C Sterilize 20min.

Wherein, the activation method of saccharomycete are as follows: saccharomycete is inoculated in the fluid nutrient medium of saccharomycete in anaerobic condition Under, revolving speed 600r/min, temperature is shaking table culture 50h under the conditions of 37 DEG C, the fluid nutrient medium of the saccharomycete by as follows at It is grouped as: yeast powder 9g/L, beef extract 12g/L, sodium bicarbonate 5g/L, sodium chloride 3g/L, aloe polysaccharide 13g/L, auxiliary agent 130mL, manioc waste leaching liquor 35g/L, molasses leaching liquor 12g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C sterilizing 20min.

Wherein, auxiliary agent mixes the preparation method comprises the following steps: considering to be worth doing Radix Isatidis, pawpaw skin, banana and Eucalyptus according to mass ratio for 1:2:6:3 It closes, the sucrose for being then 13g/L with concentration is that 3:2 is mixed according to mass ratio, is sealed in 13d in watt cylinder, filters to take supernatant i.e. ?.

Then the molasses leaching liquor of the present embodiment adds gross mass the preparation method comprises the following steps: molasses and water are mixed according to 1:2 1/3 active carbon filters to take filtrate after adsorbing 2h to obtain the final product.

Control group 1:

Without fermentation mulberry leaf in the biological feedstuff raw material of this control group, raw material is comprised the following components in parts by weight: 26 Extract of mulberry twig, 21 parts of the black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of fermentation cassava of part Slag, 14 parts of fermentation molasses and 17 parts of fermented maize palpus, other embodiment and embodiment 1 are completely the same.

Control group 2:

Fermentation cassava slag is free of in the biological feedstuff raw material of this control group, raw material comprises the following components in parts by weight: 97 parts of fermentation mulberry leaf, 26 parts of extract of mulberry twig, 21 parts of black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sugarcane Sugar, 14 parts of fermentation molasses and 17 parts of fermented maize palpus, other embodiment and embodiment 1 are completely the same.

Control group 3:

Without fermentation molasses in the biological feedstuff raw material of this control group, raw material is comprised the following components in parts by weight: 97 Part fermentation mulberry leaf, 26 parts of extract of mulberry twig, 21 parts of black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of fermentation cassava slag and 17 parts of fermented maize palpus, other embodiment and embodiment 1 are completely the same.

Control group 4:

Without fermented maize palpus in the biological feedstuff raw material of this control group, raw material comprises the following components in parts by weight 97 Part fermentation mulberry leaf, 26 parts of extract of mulberry twig, 21 parts of black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of fermentation cassava slag and 4 parts of fermentation molasses, other embodiment and embodiment 1 are completely the same.

Control group 5:

Extract of mulberry twig is free of in the biological feedstuff raw material of this control group, raw material comprises the following components in parts by weight 97 Part fermentation mulberry leaf, 21 parts of black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of fermentation cassava slag, 14 parts of fermentation molasses and 17 parts of fermented maize palpus, other embodiment and embodiment 1 are completely the same.

Control group 6:

The mulberry leaf of this control group, manioc waste, molasses, corn stigma without fermentation process, ramulus mori without extract only into Row crushes, and other preparation methods and embodiment 1 are completely the same, and biological feedstuff formula comprises the following components in parts by weight: 97 parts Mulberry leaf, 26 parts of ramulus mori powder, 21 parts of black rice, 13 parts of corn stover, 31 parts of pueraria lobata, 7 parts of sucrose, 32 parts of cassava Slag, 14 parts of molasses and 17 parts of corn stigma.

Test experiments 1: the determination of bioactive constituent of biological feedstuff:

Cyanide content (cyaniding analyte detection CN in testing example 1-3 and control group 1-6 biological feedstuff^-Content), thick egg Bai Hanliang, crude fiber content, dry matter content, total amino acid content and general flavone, are specifically shown in Table 1:

Table 1

As seen from the above table, the cyanide content of embodiment 1-3, crude fibre are significantly lower than control group 6, illustrate the feeding of the application Material formula and processing method can effectively reduce cyanide and crude fiber content in feed；The crude protein of embodiment 1-3, dry matter, Total amino acid, general flavone content are apparently higher than control group 6, illustrate that the feed formula of the application and processing method can effectively improve feeding Crude protein, dry matter, total amino acid and general flavone content in material.

Testing experiment 2: sensory evaluation:

The subjective appreciation person that sensory evaluation scores method is made of 10 feed processing related technical personnel, to different disposal sample Sensory evaluation is carried out, evaluation result is shown in Table 2:

Table 2

As seen from the above table, the smell of control group 2-3 and control group 6 is not so good as other group, illustrates the manioc waste fermentation of the application With the molasses fermented palatability and quality for effectively improving feed.

Testing experiment 3: viable count after probiotics activation

Control group A:

The probiotics of this control group directlys adopt commercially available lactic acid bacteria and saccharomycete prepares biological feedstuff without overactivation, Other preparation methods are identical with embodiment 1.

Control group B:

The lactic acid bacteria of this control group, saccharomycete activation medium do not add manioc waste leaching liquor, i.e. the liquid training of lactic acid bacteria Base is supported by as follows at being grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, auxiliary agent 100mL, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizings 20min.The fluid nutrient medium of saccharomycete is by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, chlorine Change sodium 2g/L, aloe polysaccharide 10g/L, auxiliary agent 100mL, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH To 7.0,120 DEG C of sterilizing 20min.Wherein, auxiliary agent is the preparation method comprises the following steps: considering to be worth doing Radix Isatidis, pawpaw skin, banana and Eucalyptus according to quality Than mixing for 1:1:4:2, the sucrose for being then 10g/L with concentration is that 2:1 is mixed according to mass ratio, is sealed in 10d in watt cylinder, mistake Leaching supernatant to obtain the final product；Other preparation methods are identical with embodiment 1.

Control group C:

The lactic acid bacteria of this control group, saccharomycete activation medium do not add molasses leaching liquor, the i.e. Liquid Culture of lactic acid bacteria Base is by as follows at being grouped as yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/ L, auxiliary agent 100mL, manioc waste leaching liquor 20g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizings 20min.The fluid nutrient medium of saccharomycete is by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, chlorine Change sodium 2g/L, aloe polysaccharide 10g/L, auxiliary agent 100mL, manioc waste leaching liquor 30g/L, the above ingredient dissolution, with sodium hydroxide tune PH to 7.0,120 DEG C of sterilizing 20min.Wherein, auxiliary agent is the preparation method comprises the following steps: considering to be worth doing Radix Isatidis, pawpaw skin, banana and Eucalyptus according to matter For amount than being that 1:1:4:2 is mixed, the sucrose for being then 10g/L with concentration is that 2:1 is mixed according to mass ratio, is sealed in 10d in watt cylinder, Filter to take supernatant to obtain the final product；Other preparation methods are identical with embodiment 1.

Control group D:

The lactic acid bacteria of this control group, saccharomycete activation medium do not add auxiliary agent, i.e., the fluid nutrient medium of lactic acid bacteria is by such as It is lower at being grouped as yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, cassava Slag leaching liquor 20g/L, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min. The fluid nutrient medium of saccharomycete is by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/ L, aloe polysaccharide 10g/L, manioc waste leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH To 7.0,120 DEG C of sterilizing 20min.Other preparation methods are identical with embodiment 1.

Control group E:

The lactic acid bacteria of this control group, saccharomycete activation medium use glucose substitution gumbo polysaccharide or aloe polysaccharide, i.e., The fluid nutrient medium of lactic acid bacteria is by as follows at being grouped as: i.e. the fluid nutrient medium of lactic acid bacteria is by as follows at being grouped as: yeast powder 10g/L, beef extract 10g/L, sodium citrate 2g/L, sodium chloride 2g/L, gumbo polysaccharide 10g/L, auxiliary agent 100mL, manioc waste extraction Liquid 20g/L, molasses leaching liquor 5g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min.Saccharomycete Fluid nutrient medium by as follows at being grouped as: yeast powder 7g/L, beef extract 8g/L, sodium bicarbonate 3g/L, sodium chloride 2g/L, auxiliary agent 100mL, manioc waste leaching liquor 30g/L, molasses leaching liquor 10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.0,120 DEG C sterilizing 20min.Wherein, auxiliary agent is the preparation method comprises the following steps: it is 1:1 that Radix Isatidis, pawpaw skin, banana and Eucalyptus, which are considered to be worth doing according to mass ratio: 4:2 mixing, the sucrose for being then 10g/L with concentration is that 2:1 is mixed according to mass ratio, is sealed in 10d in watt cylinder, filters to take supernatant Liquid to obtain the final product；Other preparation methods are identical with embodiment 1.

The living bacteria count of testing example 1 and control group A-E finished product feedstuff；Specifically it is shown in Table 3:

Table 3

As seen from the above table, the living bacteria count of embodiment 1 is apparently higher than control group A-control group E and illustrates that the application's is prebiotic Bacterium activation method can effectively improve the living bacteria count of biological feedstuff.

Feeding experiment:

Piglet grows into 100kg from 20kg and is known as the stage of fattening；

It is carried out at the test site of this institute, test selection age, the miscellaneous market pig of the consistent ternary of weight are divided into 8 groups, every group 10 Head, experimental stage are to deliver for sale after 20kg is fattened to 110kg.

Feeding is carried out by pig of the feed formula of test group 1-7 and control group to the stage of fattening, daily to feed 3 times, morning 6: 00-7:00, noon 12:00-13:00, afternoon 18:00-19:00；Other management methods are consistent.

Test group 1: it is fed with pig of the feed of embodiment 1 to the stage of fattening；

Test group 2: it is fed with pig of the feed of control group 1 to the stage of fattening；

Test group 3: it is fed with pig of the feed of control group 2 to the stage of fattening；

Test group 4: it is fed with pig of the feed of control group 3 to the stage of fattening；

Test group 5: it is fed with pig of the feed of control group 4 to the stage of fattening；

Test group 6: it is fed with pig of the feed of control group 5 to the stage of fattening；

Test group 7: it is fed with pig of the feed of control group 6 to the stage of fattening；

Control group: it is fed with pig of the black rice flour to the stage of fattening.

Test method and testing index

The acquisition of sample: in off-test, 3 for testing end of term live-weight close to its cell mean are selected respectively from each group Pig butchers pig after on an empty stomach 24, measures live yellowish pink, marble grain, shearing force and the protein content of pork:

Live yellowish pink measurement: colorimetric block-regulations, using six grades of U.S. Color Score plate (NPPC, USA)；

Marble grain: marble grain grading board, using six grade standard marble grain color board (NPPC, USA) of the U.S.；

Shearing force: drilling through 6-8 block meat sample along muscle fibre direction, and with domestic tenderometer, vertical muscle fibre direction measures respectively Shearing force.Shearing force is bigger, and tenderness is poorer, vice versa it is so.

Protein content determination: using Kjeldahl's method, takes fresh meat sample 6.0kg ± 1.0kg, and the concentrated sulfuric acid digests 4h, 0.05mol/L titration with hydrochloric acid calculates protein content.

Specifically it is shown in Table 4:

Table 4

Group	It is yellowish pink	Marble grain	Shearing force (kgfN)	Protein content (%)
					Test group 1	4.04	3.07	2.04	26.31
Test group 2	3.16	2.64	2.98	23.01
					Test group 3	3.01	2.31	3.06	21.36
Test group 4	3.07	2.64	3.12	18.32
					Test group 5	3.26	2.91	3.04	17.36
Test group 6	3.31	2.31	3.12	19.34
					Test group 7	3.21	2.34	3.23	17.06
Control group	2.98	2.74	3.38	12.34

As seen from the above table, the yellowish pink of test group 1, marble grain are higher than control group；Illustrate feed formula and the feeding of the application Material processing method can effectively improve the exterior quality of pork；The shearing force of test group 1 is less than control group, illustrates the feed of the application Formula and feed making method can effectively improve pork tenderness；The protein content of test group 1 is higher than control group, illustrates the application Feed formula and feed making method can effectively improve porcine protein content.

In conclusion the feed formula and preparation method of the application can reduce cyanide content in feed, improve crude protein Content reduces crude fiber content, improves dry matter, total amino acid and general flavone content；Can effectively it be changed using the feed of the application Kind animal meat, keeps animal meat fresher and tenderer, improves the protein content of pork.

Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair Bright patent claim, it is all the present invention suggested by technical spirit under completed same changes or modifications change, should all belong to In the covered the scope of the patents of the present invention.

Claims

1. a kind of mulberry leaf biological feedstuff, which is characterized in that the feed is made of following parts by weight ingredient: 97 parts -135 parts of hair Ferment mulberry leaf, 26 parts -46 parts of extract of mulberry twig, 21 parts -34 parts of black rice, 13 parts -34 parts of corn stover, 31 parts -45 parts Pueraria lobata, 7 parts -18 parts of sucrose, 32 parts -46 parts of fermentation cassava slag, 14 parts -26 parts of fermentation molasses and 17 parts -26 parts of hair Ferment corn stigma.

2. a kind of mulberry leaf biological feedstuff according to claim 1, which is characterized in that the preparation method of the extract of mulberry twig Are as follows: the belt leather ramulus mori that moisture content is 3%-5% is crushed, is then screened by the sieve of -150 mesh of 100 mesh；Then it is added 40 DEG C -50 DEG C of warm water is impregnated, when immersion a length of 2h-3h；It is put into after immersion in -20 DEG C~-10 DEG C of refrigerator-freezer and freezes 3h- 4h；It thaws, filter under room temperature after freezing, take filter residue to be divided into two parts, the second that a copy of it and percentage by volume are 60%-70% Alcoholic solution mixing, is filtered after carrying out ultrasonic extraction in supersonic extractors, and filtrate is taken to carry out rotary evaporation concentration, until concentrate Extract I is obtained for the 1/10-1/8 of stoste；Another is mixed with the butanol solution that percentage by volume is 10%-20%, is being returned It is filtered after carrying out refluxing extraction 10h-12h in stream extractor, filtrate is taken to carry out rotary evaporation concentration, until concentrate is stoste Obtain extract II；Extract I and extract II are mixed to get extract of mulberry twig.

3. a kind of mulberry leaf biological feedstuff according to claim 2, which is characterized in that the power of the ultrasonic extraction is 150w-200w；Extraction time is 10min-20min.

4. a kind of mulberry leaf biological feedstuff according to claim 2, which is characterized in that the Extracting temperature of the refluxing extraction device It is 130 DEG C -150 DEG C.

5. a kind of mulberry leaf biological feedstuff according to claim 1, which is characterized in that the fermentation process of the fermentation mulberry leaf Are as follows: by mulberry leaf cutting and complex enzyme I and water according to the inoculum concentration that mass ratio is after 50-60:1:15-20 is mixed according to 2%-5% Access activation probiotics, ferment 4d-6d in the case where temperature is 35 DEG C -38 DEG C of ventilation condition；After ventilating fermentation temperature be 36-38 DEG C carry out stack retting under confined conditions；A length of 5d-7d when stack retting obtains fermentation mulberry leaf；The complex enzyme I is by enzyme-activity unit It according to mass ratio is 1-3:2-4 that the carboxypeptidase and enzyme-activity unit of 300U/mg-800U/mg, which is 500U/mg-700U/mg cellulase, Composition.

6. a kind of mulberry leaf biological feedstuff according to claim 1, which is characterized in that the fermentation process of the fermented maize palpus Are as follows: it will be cooled to room temperature after corn stigma blanching, be then that 50-60:1-3 is mixed according to mass ratio with compound enzyme II, mixed enzymolysis 12h-14h is 36-38 DEG C in temperature then to the corn stigma after enzymatic hydrolysis according to the inoculum concentration access activation probiotics of 1%-4% Carry out stack retting under confined conditions, when stack retting a length of 5d-7d；After stack retting temperature be 33 DEG C -37 DEG C under conditions of ventilating fermentation 4d-6d obtains fermented maize palpus；The compound enzyme II is by the carboxypeptidase that enzyme-activity unit is 300U/mg-800U/mg, enzyme-activity unit The pectase for being 1000U/mg-1500U/mg for 500U/mg-700U/mg cellulase and enzyme-activity unit according to mass ratio is 1- 4:5-7:2-5 composition.

7. a kind of mulberry leaf biological feedstuff according to claim 5-6 any one, which is characterized in that the activation probiotics by Living bacteria count is 1.5 × 10⁸-4.0×10⁸The lactic acid bacteria of cfu/mg and living bacteria count are 2.5 × 10⁸-5.0×10⁸cfu/ The saccharomycete of mg is mixed to prepare according to mass ratio for 2-4:3-6；The activation method of the lactic acid bacteria are as follows: by lactobacillus inoculum in cream In the fluid nutrient medium of sour bacterium under anaerobic, revolving speed 500r/min-800r/min, shaking table is trained under the conditions of temperature is 37 DEG C Support 48-52h, the fluid nutrient medium of the lactic acid bacteria is by as follows at being grouped as: yeast powder 10-15g/L, beef extract 10-15g/L, Sodium citrate 2-5g/L, sodium chloride 2-4g/L, gumbo polysaccharide 10-15g/L, auxiliary agent 100mL-150mL, manioc waste leaching liquor 20- 30g/L, molasses leaching liquor 5-10g/L, the above ingredient dissolution, with sodium hydroxide tune pH to 7.5,120 DEG C of sterilizing 20min；It is described The activation method of saccharomycete are as follows: be inoculated in saccharomycete in the fluid nutrient medium of saccharomycete under anaerobic, revolving speed 500r/ Min-800r/min, temperature are shaking table culture 48-52h under the conditions of 37 DEG C, and the fluid nutrient medium of the saccharomycete is by following ingredient Composition: yeast powder 7-12g/L, beef extract 8-16g/L, sodium bicarbonate 3-6g/L, sodium chloride 2-4g/L, aloe polysaccharide 10-15g/ Hydrogen-oxygen is used in L, auxiliary agent 100mL-150mL, manioc waste leaching liquor 30-40g/L, molasses leaching liquor 10-15g/L, the above ingredient dissolution Change sodium tune pH to 7.0,120 DEG C of sterilizing 20min.

8. a kind of mulberry leaf biological feedstuff according to claim 7, which is characterized in that the auxiliary agent is the preparation method comprises the following steps: by plate indigo plant Root, pawpaw skin, banana and Eucalyptus bits are 1:1-3:4-7:2-4 mixing according to mass ratio, are then 10g/L-15g/L's with concentration Sucrose is 2-4:1-3 mixing according to mass ratio, is sealed in 10d-15d in watt cylinder, filters to take supernatant to obtain the final product.

9. any one of the claim 1-8 method for preparing mulberry leaf biological feedstuff, which is characterized in that the method includes as follows Step:

(1) each raw material is weighed by the parts by weight；

(2) will fermentation mulberry leaf, pueraria lobata, black rice, sucrose, fermentation cassava slag, fermentation molasses and fermented maize must mix, 35 DEG C- Stack retting 2d-3d under the conditions of 37 DEG C；Then steam sterilizing 40min-50min under the conditions of 120 DEG C -130 DEG C；Then -4 DEG C~0 10min is freezed under the conditions of DEG C, obtains the mixture of fermentation material；

(3) corn straw smashing is crossed after the screening of -150 mesh screen of 100 mesh to the fermentation material mixture for being then added to step (2), so It sprays in backward mixture after extract of mulberry twig mixes and constantly sprays water until mixture hand pinches agglomerating into mixture again, Stack retting 3d-4d under the conditions of 36 DEG C -38 DEG C, drying obtains the biological feedstuff of the application under the conditions of 60 DEG C -70 DEG C.

10. the mulberry leaf biological feedstuff of preparation method described in the mulberry leaf biological feedstuff or claim 9 of any one of claim 1-8 exists Improve the purposes in porcine protein content.