CN109463225B - Method for screening drought-resistant coix seed variety - Google Patents

Method for screening drought-resistant coix seed variety Download PDF

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CN109463225B
CN109463225B CN201811609125.9A CN201811609125A CN109463225B CN 109463225 B CN109463225 B CN 109463225B CN 201811609125 A CN201811609125 A CN 201811609125A CN 109463225 B CN109463225 B CN 109463225B
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coix
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root length
drought
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杜磊
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Yuncheng University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
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    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/30Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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Abstract

The invention relates to a method for screening drought-enduring coix seeds, which sequentially comprises the steps of organic ecological culture medium formula development, drought-enduring variety identification and screening, drought resistance value calculation, drought-enduring ability identification and the like. According to the method for screening drought-resistant coix seed varieties, the influence of matrixes with different formulas on the growth vigor, plant photosynthetic indexes, root systems, yield and quality of coix seeds is researched, the optimal formula of the organic ecological coix seed cultivation matrix is determined, and compared with field planting, the cultivation matrix is good in plant growth vigor and capable of improving yield and quality, on the one hand, the use of chemical fertilizers and pesticides can be reduced, and water and fertilizer conservation can be achieved.

Description

Method for screening drought-resistant coix seed variety
Technical Field
The invention relates to the field of crop planting, in particular to a method for screening drought-resistant coix seed varieties.
Background
Coix lacryma-jobi is a plant of Gramineae, Coix, annual stout herbaceous plant, and has yellow and white fibrous root, spongy substance, and diameter of about 3 mm. The flower and fruit period is 6-12 months. Mainly produced in the provinces of Hubei, Qi Chun, Hunan, Hebei, Jiangsu, Fujian, etc. in China. The coix seed is one of the traditional Chinese food resources, and can be made into porridge, rice and various cooked wheaten foods for people to eat. Especially suitable for the old and the weak. Sweet and bland in flavor, slightly cold in nature. The Chinese yam, the four-river village, the most famous of the origin, has the functions of strengthening spleen, promoting diuresis, clearing heat, expelling pus, maintaining beauty and keeping young.
For coix, the development degree of underground root system determines the strength of drought resistance. Drought resistance of different varieties of the same coix can be identified according to the development degree of underground root systems, but the development degree of the root systems is greatly influenced by the matrix. The culture medium is more suitable for the growth of the root system of the coix seed than soil, for example, the total porosity of the culture medium is larger than that of the soil medium. In order to create a good growing environment for root systems, a cultivation substrate suitable for coix is firstly required to be developed.
Disclosure of Invention
One purpose of the invention is to provide a method for screening drought-enduring coix seed varieties.
The invention discloses a method for screening drought-resistant coix seed varieties, which comprises the following steps: s101: crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: (3.5-4.5): (1.5-2.5): (2.5-3.5) and (2.5-3.5), mixing the mixture with carbendazim according to the weight ratio of 10L: (0.8 g-1.2 g) and fermenting and decomposing to obtain the organic ecological culture medium; s102: selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds in soil at a first preset row spacing into 9 cultivation grooves in a point sowing mode, and covering the organic ecological cultivation substrate obtained in the step S101 on the sowed cultivation grooves; sowing a coix seed variety in each 3 cultivation grooves, wherein each group comprises 1 variety, and is totally divided into 3 groups, the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3; s103: thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance; s104: treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity; treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity; treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity; then, the first relative humidity, the second relative humidity and the third relative humidity are sequentially reduced to a fourth relative humidity in a step mode within a preset time, and then the fourth relative humidity is kept for 13-16 days to carry out drought treatment; s105: counting the main root length of each coix in the 3 groups of cultivation grooves, then calculating the average main root length of each coix in each cultivation groove, then calculating the average main root length of each coix in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of the treatment 2 is equal to the average root length of the coix of the treatment 2/the average root length of the coix of the treatment 3, and the formula (2) is shown in the specification;
s106: calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
According to the method for screening drought-resistant coix seed varieties, the influence of matrixes with different formulas on the growth vigor, plant photosynthetic indexes, root systems, yield and quality of coix seeds is researched, the optimal formula of the organic ecological coix seed cultivation matrix is determined, and compared with field planting, the cultivation matrix is good in plant growth vigor and capable of improving yield and quality, on the one hand, the use of chemical fertilizers and pesticides can be reduced, and water and fertilizer conservation can be achieved.
In addition, the method for screening drought-enduring coix seeds can also have the following additional technical characteristics:
further, in the step S102, the length of the cultivation groove is 45cm to 55cm, the width is 45cm to 55cm, and the depth is 25cm to 35 cm.
Further, in the step S102, the first preset planting distance is 1cm to 3 cm.
Further, in the step S102, the thickness of the organic ecological culture medium is 1.5cm to 2.5 cm.
Further, in the step S103, the second preset planting distance is 4cm to 6 cm.
Further, in the step S102 and the step S103, the relative humidity of the organic ecological culture medium of all the culture tanks is 70% to 80%.
Further, in the step S104, the first relative humidity is 28% to 32%; the second relative humidity is 72% -78%; the third relative humidity is 72-78%; the fourth relative humidity is 28% -32%.
Further, in the step S104, the preset time is 2 days to 10 days.
Further, the step S102 is performed in a greenhouse having an air relative humidity of 80% and a temperature of 25 ℃.
Further, in the step S105, when the main root length of each coix is measured, the main root length of each coix is obtained by averaging the measurements three times for each coix.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The following detailed description of embodiments of the invention is intended to be illustrative, and not to be construed as limiting the invention.
The invention discloses a method for screening drought-resistant coix seed varieties, which comprises the following steps:
s101: crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: (3.5-4.5): (1.5-2.5): (2.5-3.5) and (2.5-3.5), mixing the mixture with carbendazim according to the weight ratio of 10L: (0.8 g-1.2 g) and fermenting and decomposing to obtain the organic ecological culture medium. The organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.75g/cm3The total porosity was 72%, the gas permeability pore was 11%, the water retention pore was 61%, the pH was 7.1, the conductivity was 2.97ms/cm, and the cation exchange capacity was 55c mol/kg.
S102: selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds into 9 cultivation grooves in soil in a point sowing mode according to a first preset row spacing, and covering the organic ecological cultivation substrate obtained in the step S101 on the sowed cultivation grooves; one coix seed variety is sowed in each 3 cultivation grooves, each group comprises 1 variety, and is divided into 3 groups, wherein the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3. The length of the cultivation groove is 45-55 cm, the width is 45-55 cm, and the depth is 25-35 cm; the first preset planting distance is 1 cm-3 cm; the thickness of the organic ecological culture medium is 1.5 cm-2.5 cm. Said step S102 is carried out in a greenhouse with an air relative humidity of 80% and a temperature of 25 ℃.
S103: thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance. The second preset planting distance is 4 cm-6 cm. In the step S102 and the step S103, the relative humidity of the organic ecological culture medium of all the culture tanks is 70% to 80%.
S104: treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity; treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity; treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity; and then, the first relative humidity, the second relative humidity and the third relative humidity are sequentially reduced to a fourth relative humidity in a step mode within 2-10 days, and then the fourth relative humidity is kept for 13-16 days to carry out drought treatment. The first relative humidity is 28% -32%; the second relative humidity is 72% -78%; the third relative humidity is 72-78%; the fourth relative humidity is 28% -32%.
S105: counting the main root length of each coix seed in the 3 groups of cultivation grooves, measuring each coix seed for three times to obtain an average value, then calculating the average main root length of each coix seed in each cultivation groove, then calculating the average main root length of each coix seed in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of treatment 2 is the average root length of the coix of treatment 2/the average root length of the coix of treatment 3, and is expressed by the formula (2).
The data processing mode is similar to the calculation of the drought resistance coefficient, except that the experiment replaces the yield in the drought resistance coefficient calculation formula with the main root length. For the accuracy of drought resistance identification, when the drought resistance value of a single coix seed variety is calculated, a relative value is adopted so as to eliminate background interference caused by a culture medium. The larger the drought resistance value is, the stronger the drought resistance is.
S106: calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
The present invention is described in detail below by way of specific examples.
The method for screening drought-enduring coix seed varieties in embodiment 1 comprises the following steps:
(1) crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: 3.5: 2.5: 2.5:3.5, and then mixing the mixture with carbendazim according to a ratio of 10L: mixing the components in a ratio of 0.8g, and fermenting and decomposing to obtain the organic ecological culture medium. The organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.75g/cm3The total porosity was 72%, the gas permeability pore was 11%, the water retention pore was 61%, the pH was 7.1, the conductivity was 2.97ms/cm, and the cation exchange capacity was 55c mol/kg.
(2) Selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds into 9 cultivation grooves in soil in a point sowing mode according to a first preset row spacing, and covering the organic ecological cultivation substrate obtained in the step (1) on the sowed cultivation grooves; one coix seed variety is sowed in each 3 cultivation grooves, each group comprises 1 variety, and is divided into 3 groups, wherein the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3. The length of the cultivation groove is 55cm, the width is 45cm, and the depth is 35 cm; the first preset planting distance is 1 cm; the thickness of the organic ecological culture medium is 2.5 cm. The step (2) is carried out in a greenhouse with the relative humidity of air of 80 percent and the temperature of 25 ℃.
(3) Thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance. The second preset planting distance is 4 cm. In the step (2) and the step (3), the relative humidity of the organic ecological culture medium of all the culture tanks is 80%.
(4) Treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity; treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity; treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity; and then, the first relative humidity, the second relative humidity and the third relative humidity are sequentially reduced to a fourth relative humidity in a stepwise manner within 2 days, and then the fourth relative humidity is maintained for 16 days to carry out drought treatment. The first relative humidity is 28%; the second relative humidity is 78%; the third relative humidity is 72%; the fourth relative humidity is 32%.
(5) Counting the main root length of each coix seed in the 3 groups of cultivation grooves, measuring each coix seed for three times to obtain an average value, then calculating the average main root length of each coix seed in each cultivation groove, then calculating the average main root length of each coix seed in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of treatment 2 is the average root length of the coix of treatment 2/the average root length of the coix of treatment 3, and is expressed by the formula (2).
The data processing mode is similar to the calculation of the drought resistance coefficient, except that the experiment replaces the yield in the drought resistance coefficient calculation formula with the main root length. For the accuracy of drought resistance identification, when the drought resistance value of a single coix seed variety is calculated, a relative value is adopted so as to eliminate background interference caused by a culture medium. The larger the drought resistance value is, the stronger the drought resistance is.
(6) Calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
The method for screening drought-enduring coix seed varieties in embodiment 2 comprises the following steps:
(1) crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: 4.5: 1.5: 3.5:2.5, and then mixing the mixture with carbendazim according to a ratio of 10L: mixing the raw materials in a proportion of 1.2g, and fermenting and decomposing to obtain the organic ecological culture medium.The organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.75g/cm3The total porosity was 72%, the gas permeability pore was 11%, the water retention pore was 61%, the pH was 7.1, the conductivity was 2.97ms/cm, and the cation exchange capacity was 55c mol/kg.
(2) Selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds into 9 cultivation grooves in soil in a point sowing mode according to a first preset row spacing, and covering the organic ecological cultivation substrate obtained in the step (1) on the sowed cultivation grooves; one coix seed variety is sowed in each 3 cultivation grooves, each group comprises 1 variety, and is divided into 3 groups, wherein the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3. The length of the cultivation groove is 45cm, the width is 55cm, and the depth is 25 cm; the first preset planting distance is 3 cm; the thickness of the organic ecological culture medium is 1.5 cm. The step (2) is carried out in a greenhouse with the relative humidity of air of 80 percent and the temperature of 25 ℃.
(3) Thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance. The second preset planting distance is 6 cm. In the step (2) and the step (3), the relative humidity of the organic ecological culture medium of all the culture tanks is 70%.
(4) Treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity; treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity; treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity; and then, the first relative humidity, the second relative humidity and the third relative humidity are sequentially reduced to a fourth relative humidity in a step mode within 10 days, and then the fourth relative humidity is kept for 13 days to carry out drought treatment. The first relative humidity is 32%; the second relative humidity is 72%; the third relative humidity is 78%; the fourth relative humidity is 28%.
(5) Counting the main root length of each coix seed in the 3 groups of cultivation grooves, measuring each coix seed for three times to obtain an average value, then calculating the average main root length of each coix seed in each cultivation groove, then calculating the average main root length of each coix seed in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of treatment 2 is the average root length of the coix of treatment 2/the average root length of the coix of treatment 3, and is expressed by the formula (2).
The data processing mode is similar to the calculation of the drought resistance coefficient, except that the experiment replaces the yield in the drought resistance coefficient calculation formula with the main root length. For the accuracy of drought resistance identification, when the drought resistance value of a single coix seed variety is calculated, a relative value is adopted so as to eliminate background interference caused by a culture medium. The larger the drought resistance value is, the stronger the drought resistance is.
(6) Calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
The method for screening drought-enduring coix seed varieties in embodiment 3 comprises the following steps:
(1) crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: 4: 2: 3: 3, and then mixing the mixture with carbendazim according to a ratio of 10L: mixing the raw materials in a proportion of 1g, and fermenting and decomposing to obtain the organic ecological culture medium. The organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.75g/cm3The total porosity was 72%, the gas permeability pore was 11%, the water retention pore was 61%, the pH was 7.1, the conductivity was 2.97ms/cm, and the cation exchange capacity was 55c mol/kg.
(2) Selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds into 9 cultivation grooves in soil in a point sowing mode according to a first preset row spacing, and covering the organic ecological cultivation substrate obtained in the step (1) on the sowed cultivation grooves; one coix seed variety is sowed in each 3 cultivation grooves, each group comprises 1 variety, and is divided into 3 groups, wherein the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3. The length of the cultivation groove is 50cm, the width of the cultivation groove is 50cm, and the depth of the cultivation groove is 30 cm; the first preset planting distance is 2 cm; the thickness of the organic ecological culture medium is 2 cm. The step (2) is carried out in a greenhouse with the relative humidity of air of 80 percent and the temperature of 25 ℃.
(3) Thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance. The second preset planting distance is 5 cm. In the step (2) and the step (3), the relative humidity of the organic ecological culture medium of all the culture tanks is 75%.
(4) Treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity; treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity; treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity; and then, the first relative humidity, the second relative humidity and the third relative humidity are reduced to a fourth relative humidity in a stepwise manner within 5 days in sequence, and then the fourth relative humidity is maintained for 15 days to carry out drought treatment. The first relative humidity is 30%; the second relative humidity is 75%; the third relative humidity is 75%; the fourth relative humidity is 30%.
(5) Counting the main root length of each coix seed in the 3 groups of cultivation grooves, measuring each coix seed for three times to obtain an average value, then calculating the average main root length of each coix seed in each cultivation groove, then calculating the average main root length of each coix seed in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of treatment 2 is the average root length of the coix of treatment 2/the average root length of the coix of treatment 3, and is expressed by the formula (2).
The data processing mode is similar to the calculation of the drought resistance coefficient, except that the experiment replaces the yield in the drought resistance coefficient calculation formula with the main root length. For the accuracy of drought resistance identification, when the drought resistance value of a single coix seed variety is calculated, a relative value is adopted so as to eliminate background interference caused by a culture medium. The larger the drought resistance value is, the stronger the drought resistance is.
(6) Calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
According to the method for screening drought-resistant coix seed varieties, the influence of matrixes with different formulas on the growth vigor, plant photosynthetic indexes, root systems, yield and quality of coix seeds is researched, the optimal formula of the organic ecological coix seed cultivation matrix is determined, and compared with field planting, the cultivation matrix is good in plant growth vigor and capable of improving yield and quality, on the one hand, the use of chemical fertilizers and pesticides can be reduced, and water and fertilizer conservation can be achieved.
In the description herein, references to the description of the term "one embodiment," "some embodiments," "an example," "a specific example," or "some examples," etc., mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, various embodiments or examples and features of different embodiments or examples described in this specification can be combined and combined by one skilled in the art without contradiction.
Although embodiments of the present invention have been shown and described above, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art within the scope of the present invention.

Claims (9)

1. A method for screening drought-enduring coix seed varieties is characterized by comprising the following steps:
s101: crushing corn straws and bones, and then mixing the corn straws, decomposed cow dung, bone residues and wormcast in a weight ratio of 1: (3.5-4.5): (1.5-2.5): (2.5-3.5) and (2.5-3.5), mixing the mixture with carbendazim according to the weight ratio of 10L: (0.8 g-1.2 g) and fermenting and decomposing to obtain the organic ecological culture medium;
s102: selecting and disinfecting A, B, C3 varieties of coix seeds with full and uniform seeds and no diseases, sowing the seeds in soil at a first preset row spacing into 9 cultivation grooves in a point sowing mode, and covering the organic ecological cultivation substrate obtained in the step S101 on the sowed cultivation grooves; sowing a coix seed variety in each 3 cultivation grooves, wherein each group comprises 1 variety, and is totally divided into 3 groups, the first group comprises A1, A2 and A3, the second group comprises B1, B2 and B3, and the third group comprises C1, C2 and C3;
s103: thinning after the coix seed sprouts and leaves grow, keeping the growth vigor consistent and removing redundant coix seeds within the range of a second preset planting distance;
s104: treating A1, B1 and C1 by adjusting the organic ecological culture medium to a first relative humidity;
treatment 2 for a2, B2, C2: adjusting the organic ecological culture medium to a second relative humidity;
treatment 3 of a3, B3, C3: adjusting the organic ecological culture medium to a third relative humidity;
then, the first relative humidity, the second relative humidity and the third relative humidity are sequentially reduced to a fourth relative humidity in a step mode within a preset time, and then the fourth relative humidity is kept for 13-16 days to carry out drought treatment;
the first relative humidity is 28% -32%; the second relative humidity is 72% -78%;
the third relative humidity is 72-78%; the fourth relative humidity is 28% -32%;
s105: counting the main root length of each coix in the 3 groups of cultivation grooves, then calculating the average main root length of each coix in each cultivation groove, then calculating the average main root length of each coix in each processed cultivation groove, and then calculating the relative main root lengths of the processing 1 and the processing 2 according to a formula (1) and a formula (2);
the relative root length of the coix of the treatment 1 is equal to the average root length of the coix of the treatment 1/the average root length of the coix of the treatment 3, and the formula (1) is shown in the specification;
the relative root length of the coix of the treatment 2 is equal to the average root length of the coix of the treatment 2/the average root length of the coix of the treatment 3, and the formula (2) is shown in the specification;
s106: calculating the drought resistance of the coix of each variety according to a formula (3);
drought resistance value of each variety of coix (relative major root length of coix of this variety treatment 1/relative major root length of coix of all varieties treatment 1) × (relative major root length of coix of this variety treatment 1/relative major root length of coix of this variety treatment 2) formula (3).
2. The method for screening drought-enduring coix seed variety according to claim 1, wherein in step S102, the cultivation groove has a length of 45cm to 55cm, a width of 45cm to 55cm, and a depth of 25cm to 35 cm.
3. The method for screening the drought-enduring coix variety according to claim 2, wherein, in the step S102, the first preset planting distance is 1 cm-3 cm.
4. The method for screening drought-enduring coix lacryma-jobi variety according to claim 1, wherein in step S102, the thickness of the organic ecological cultivation medium is 1.5 cm-2.5 cm.
5. The method for screening the drought-enduring coix variety according to claim 1, wherein, in the step S103, the second preset planting distance is 4cm to 6 cm.
6. The method for screening drought-enduring coix lacryma-jobi variety according to claim 1, wherein in steps S102 and S103, the relative humidity of the organic ecological cultivation medium of all cultivation tanks is 70-80%.
7. The method for screening drought-enduring coix lacryma-jobi varieties according to claim 1, wherein in the step S104, the preset time is 2 days to 10 days.
8. The method for screening drought-enduring coix lacryma-jobi varieties according to claim 1, wherein the step S102 is performed in a greenhouse with an air relative humidity of 80% and a temperature of 25 ℃.
9. The method of claim 1, wherein in step S105, the main root length of each coix is measured by averaging three times for each coix.
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