CN103650948A - Identification method for soybean drought resistance - Google Patents
Identification method for soybean drought resistance Download PDFInfo
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- CN103650948A CN103650948A CN201310695647.6A CN201310695647A CN103650948A CN 103650948 A CN103650948 A CN 103650948A CN 201310695647 A CN201310695647 A CN 201310695647A CN 103650948 A CN103650948 A CN 103650948A
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Abstract
Potting is carried out inside a drought-resisting greenhouse. Soybeans are drought-resisting soybeans and non-drought-resisting soybeans in variety respectively. The soil water content is controlled in the flowering phase of the soybeans, the water content of soil, where the drought-resisting soybeans and the non-drought-resisting soybeans grow, in a water control pool is continuously measured, the soybeans growing under the condition that the soil water content is 20% are used as a contrast, the minimum water content, namely 10%, of the soil where the yield reduction difference of the non-drought-resisting soybeans is not remarkable is used as the initial soil water content for controlling water, and the maximum water content, namely 5%, of the soil where the yield reduction difference of the drought-resisting soybeans is remarkable is used as the terminal water soil content for controlling the water. When the soil water continuously changes to the terminal water content, namely 5%, a morphological index chlorophyll content remarkably relevant to the yield and physical index bound water content or the difference significance of changes of the proline content are used as the effective method for identifying whether the soybeans have the drought resistance or not.
Description
Technical field
The present invention relates to a kind of authentication method of crops drought resistance, especially the authentication method to soybean drought resisting.
Background technology
Due to the pernicious variation of global climate in recent years, arid has formed serious threat to grain-production.Soybean is the important cereal crops of China, and the adverse circumstances such as arid are the bottleneck problems of restriction soybean main producing region, Northeast China yield and quality always.The drought-resistant ability of soybean is himself hereditary capacity and the coefficient result of external environment condition.Under drought condition existence and the ability that forms fructification be plant in very long evolutionary process to arid opposing and adaptation, through research at present clear and definite plant can resist arid adverse effect by escaping and self forming drought-enduring mechanism.Current many researchers cause the physiology and morphology impact of soybean to carry out a large amount of research on arid.
Impact about form aspect concentrates on: (1) reduces amount of growth: soybean plant height, branch amount, blade and cane dry weight, seed fresh weight, root fresh weight, root nodule fresh weight all strengthen and decline with drought stress intensity; (2) reduce Yield And Yield Components: under drought stress, the average pod number of the every strain of soybean, every strain average particle number and 100-grain weight all have reduction in various degree; (3) affecting Root morphology builds up: Root System In Soybean Cultivars form is at the different times of growth and in the same period not quite identical under varying environment condition.Different in moisture is coerced down, and the morphological indexs such as the main root length of soybean root system, root volume have significant reaction to arid.Show as: the drought stress in seedling stage, Soybean Root volume, root footpath, root elongation, root system total surface area have the trend of increase, and other, drought stress all caused Root morphology to grow to slow down in period.
Impact about physiology aspect concentrates on: (1) suppresses the photosynthesis of soybean: under drought stress, Photosynthesis in Soybean rate reduction, especially reduces amplitude with branching stage and drum grain phase maximum.In addition, seedling stage, transpiration rate, the stomatal conductance of plant all reduced due to drought stress, and along with the prolongation of drought stress time, chlorophyll content and leaf water content also present reduction trend.When arid soybean can keep vigorous photosynthesis ability be the important indicator of its drought resistance of judgement.(2) Plant Leaves water content declines; (3) soluble sugar content raises; (4) cell membrane property rising relatively thoroughly; (5) in cell, some enzymic activitys increase: under drought stress, catalase, peroxidase, superoxide dismutase and mda content raise gradually, when resisting drought stress, play an important role.
Research about soybean drought resisting is at present under drought stress, be that physiology and the morphological index that soil water content is controlled under a certain numerical value measured, this can illustrate to a certain extent soybean under drought stress condition on physiology and morphology to the replying of arid, and morphological index and physical signs can reflect that soybean is to arid adaptive capacity more intuitively.Yet in research process, soil moisture under drought stress is artificial setting, can not under other soil water content condition, whether there is obvious physiology and morphology change by accurate response soybean, its reason is under field growing conditions, soil moisture is change continuously, be that under drought stress, soil water content reduces gradually, and the physiology that soybean reduces moisture and the reaction of form are also to change gradually, until this change reaches significance level.Therefore, how to control the initial soil water content of drought stress and stop soil water content and duration thereof, in the duration of simultaneously and moisture dynamic change interval in this soil moisture of drought stress, measure the physiology and morphology index variation of soybean, can be used as the concrete grammar of Identification of Drought.
Summary of the invention
Drought stress, be that soil moisture content continues under reduction condition, can to arid, to produce sensitive crucial physical signs, morphological index of replying be to soybean drought resisting variety source and the transgenic drought-resistant soybean of researching and developing at present carries out the effective method of Identification of Drought, can clear and definite different cultivars resource tolerate the drought resistance degree under soil minimum water content and continuous drought, and accurately be reflected by physiology and morphology index.We are in scientific research in recent years, invented a kind of under monitoring soil moisture changes continuously, by determining, control the initial soil water content of drought stress and stop soil water content and duration thereof, thereby setting up a kind of method that soybean drought resisting is identified.
embodiment:in drought resisting canopy, carry out potted plant, kind is respectively drought resisting soybean and not drought resisting soybean, at soybean seedling and florescence, control soil water content respectively, when soil water content is 20%, the moisture of soil every day in planting with POGO II moisture automatic measuring system METHOD FOR CONTINUOUS DETERMINATION control basin, the soybean that the soil moisture content of take is grown under 20% condition is contrast, then getting soybean plant strain every day measures: morphological index, comprises root system fresh weight and dry weight, overground part fresh weight and dry weight, chlorophyll content in leaf blades, stomatal conductance; Physical signs, comprises blade Free water and fetter waetr content, proline content; During results, measure soybean yields; Analyzing soil water content reduces compared with the control under condition continuously, the significance of difference that each physiology and morphology index changes and with the correlation of change of production, thereby obtain not drought resisting soybean underproduction difference minimum soil moisture content 10% and the soil maximum water content 5% of drought resisting soybean underproduction significant difference and duration of change of moisture content thereof significantly time.When carrying out soybean drought resisting evaluation, take the inapparent soil minimum water content 10% of not drought resisting soybean underproduction difference as controlling the initial soil moisture content of moisture, using the soil maximum water content 5% of drought resisting soybean underproduction significant difference as stopping control water soil moisture content, using with the morphological index chlorophyll content of output significant correlation and physical signs bound water content or proline content as soybean drought resisting identification of indicator.
Utilize transgenic drought-resistant soybean and acceptor soybean as supplying examination material, through years of researches and practical application, show: at soybean bloom, when acceptor soybean is reduced to the soil maximum water content 5% of transgenic drought-resistant soybean underproduction significant difference from the inapparent soil minimum water content 10% of underproduction difference, duration is while being 10 days, acceptor soybean yields significantly reduces, and morphological index chlorophyll content and physical signs bound water content or all significantly reductions of proline content with output significant correlation, and transgenic drought-resistant soybean yields, chlorophyll content and bound water content are without marked change, proline content significantly increases.Therefore, available soil moisture content 10% is starting point, soil moisture content is reduced to 5% for terminal, during 10 days duration to the, measure the significance of difference that soybean morphological index chlorophyll content and physical signs bound water content or proline content change, as identifying whether soybean has the effective ways (specifically in Table 1) of drought resistance.
Table 1 soybean bloom Drought-resistance
Note: lowercase represents the significance of difference under 5% level.
Claims (1)
1. in drought resisting canopy, at the soybean florescence, control soil water content, the moisture of METHOD FOR CONTINUOUS DETERMINATION control pond medium drought resistant soybean and not drought resisting soybeans they grow soil, the soybean that the soil moisture content of take is grown under 20% condition is contrast, take the inapparent soil minimum water content 10% of not drought resisting soybean underproduction difference as controlling the initial soil moisture content of moisture, using the soil maximum water content 5% of drought resisting soybean underproduction significant difference as stopping control water soil moisture content, when soil moisture continues to change to termination water content 5%, using and as evaluation soybean, whether there are the effective ways of drought resistance with the morphological index chlorophyll content of output significant correlation and the significance of difference of physical signs bound water content or proline content variation.
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| CN201310695647.6A CN103650948A (en) | 2013-12-18 | 2013-12-18 | Identification method for soybean drought resistance |
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| CN201310695647.6A CN103650948A (en) | 2013-12-18 | 2013-12-18 | Identification method for soybean drought resistance |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393814A (en) * | 2015-10-13 | 2016-03-16 | 黑龙江八一农垦大学 | Alfalfa drought tolerance identification method |
| CN105917990A (en) * | 2016-04-17 | 2016-09-07 | 山西省农业科学院经济作物研究所 | Identification method for drought resistance of soybean |
| CN109463245A (en) * | 2018-12-27 | 2019-03-15 | 运城学院 | A method of screening drought-enduring soybean varieties |
| CN109463225A (en) * | 2018-12-27 | 2019-03-15 | 运城学院 | A method of screening drought-enduring coix lacryma-jobi kind |
| CN110736811A (en) * | 2019-11-19 | 2020-01-31 | 上海应用技术大学 | Petunia PhERF2 transgenic strain and waterlogging tolerance identification method of wild type thereof |
| CN111157575A (en) * | 2020-02-24 | 2020-05-15 | 上海应用技术大学 | Method for screening drought-resistant hemerocallis fulva based on leaf surface temperature of hemerocallis fulva |
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| CN1616964A (en) * | 2003-11-10 | 2005-05-18 | 尹伟伦 | Quantitatice evaluation technology for plant drought tolerance |
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Patent Citations (2)
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| CN1616964A (en) * | 2003-11-10 | 2005-05-18 | 尹伟伦 | Quantitatice evaluation technology for plant drought tolerance |
| CN1836494A (en) * | 2005-03-25 | 2006-09-27 | 罗利军 | System for implementing soil moisture management and moisture gradient authentication |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105393814A (en) * | 2015-10-13 | 2016-03-16 | 黑龙江八一农垦大学 | Alfalfa drought tolerance identification method |
| CN105917990A (en) * | 2016-04-17 | 2016-09-07 | 山西省农业科学院经济作物研究所 | Identification method for drought resistance of soybean |
| CN109463245A (en) * | 2018-12-27 | 2019-03-15 | 运城学院 | A method of screening drought-enduring soybean varieties |
| CN109463225A (en) * | 2018-12-27 | 2019-03-15 | 运城学院 | A method of screening drought-enduring coix lacryma-jobi kind |
| CN110736811A (en) * | 2019-11-19 | 2020-01-31 | 上海应用技术大学 | Petunia PhERF2 transgenic strain and waterlogging tolerance identification method of wild type thereof |
| CN111157575A (en) * | 2020-02-24 | 2020-05-15 | 上海应用技术大学 | Method for screening drought-resistant hemerocallis fulva based on leaf surface temperature of hemerocallis fulva |
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Application publication date: 20140326 |