CN1616964A - Quantitatice evaluation technology for plant drought tolerance - Google Patents
Quantitatice evaluation technology for plant drought tolerance Download PDFInfo
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- CN1616964A CN1616964A CN 200310113317 CN200310113317A CN1616964A CN 1616964 A CN1616964 A CN 1616964A CN 200310113317 CN200310113317 CN 200310113317 CN 200310113317 A CN200310113317 A CN 200310113317A CN 1616964 A CN1616964 A CN 1616964A
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Abstract
The quantitative evaluation technology for plant drought tolerance features that plant drought tolerance limit is measured based on the relation between plant cell membrane damage degree and soil drought stress. After gradient drought and irrigation, the turning point for plant cell membrane to have repair capacity is measured and the corresponding soil water potential is used as the drought stress death point and as the data for evaluating plant's drought tolerance. The present invention may be used in determining proper plantation range correctly.
Description
Up to now, the drought tolerance in plants ability all has no idea to carry out quantitative evaluation, all documents of forefathers all can only carry out drought-enduring with sensation to plant by rule of thumb, more drought-enduring or drought susceptible fuzzy evaluation, and in China western part and vast arid, in the work of planting trees and grass in the semiarid zone, soil moisture is the dominant factor whether restriction afforestation survives, therefore, plant species to this area is selected, primary is to select drought tolerance in plants capacity limit value must be lower than the annual minimum soil water supply ability of this area's soil, may ensure that just nursery stock spends the dry period smoothly, guarantee to survive.And the water supply capacity of this point is represented with this soil water potential.Therefore, the agricultural scholar looks forward to the drought-enduring ultimate value of seeking each kind of plant of mensuration both at home and abroad, with this all drought tolerance in plants abilities are carried out quantitative evaluation and ordering, this just provides quantitative foundation for the selection of arid, semiarid zone cultivated plant kind, guarantee that for planting trees and grass higher survival rate provides a kind of science and technology of quantification, has great economic benefit and ecological benefits.Therefore, when this inventive features is that plant is subjected to drought stress, damaged membrane, as long as root system still has vitality, after soil was poured water, the cell membrane of damage just can be repaired.This cell membrane damage degree changes and changes along with the drought stress degree, both have correlationship, but when plant run into above it the arid of the drought-enduring limit and root system when dead, after soil is poured water, the damage of plant cell membrane also no longer recovers, and then the two correlationship has not just existed.Turning to the initial tr pt of irrelevant relation from this correlationship just must be the drought-enduring ultimate value of plant, puts corresponding soil water potential is restrained oneself arid ability as the quantitative evaluation plant foundation with this.
According to this principle and method, realize that the determination step of plant drought ability quantitative evaluation is as follows:
1. select the plant individual of the identical and representative container field planting of specification for use according to claim 1., utilization stop the to pour water difference of fate makes each container constitute soil drought to coerce gradient, measure the soil water potential and the corresponding plant cell membrane degree of injury of each container.
2. working as the soil drought gradient arrives to a certain degree, measure the soil water potential of each container and the cell membrane damage degree of corresponding plants, fully pour water afterwards, measure the variation tendency of each plant cell membrane repair ability after 48 hours, thereby find out the critical point that the reparation of plant cell membrane is the slowest and can not repair.The pairing native soil flow of water of this critical point is the ultimate value of drought tolerance in plants, is worth index as the quantitative evaluation of plant drought ability with this.
The application of this invention can solve the drought tolerance in plants ability can only carry out the problem that drought-enduring, more drought-enduring, drought susceptible fuzzy non-quantitation evaluation brings with sense in the past, avoided effectively on arid lands that blindly plantation, subjectivity are introduced a fine variety the very big loss that causes manpower and materials; In addition, at the early stage quantitative screening of drought resistance breeding of new variety, excellent strain with identify and changeing in the drought-enduring gene breeding, the aspects such as quantitative evaluation of drought resisting gene function expression had crucial practical value.This technical measurement reliable results, with low cost, easy to operate, application potential is big, ecological and economic benefit height.
Claims (5)
1. the present invention relates to the quantitative evaluation technology of a drought tolerance in plants ability, when it is characterized in that plant is subjected to drought stress, damaged membrane, then membrane permeability increases, this cell membrane damage degree changes and changes along with the drought stress degree, both have correlationship, but when plant run into above it the arid of the drought-enduring limit and during root system death, the two correlationship has not just existed.Turning to the initial tr pt of irrelevant relation from this correlationship just must be the drought-enduring ultimate value of plant, puts corresponding soil water potential is restrained oneself arid ability as the quantitative evaluation plant foundation with this.
2. the correlationship of utilizing plant to be subjected to drought stress degree and cell membrane damage degree according to claim 1. is measured the method for the drought tolerance in plants limit.
3. be by after being subjected to drought gradient is coerced in various degree plant rehydration according to the mensuration of claim 1. plant drought stress killing point, have according to plant cell membrane that the pairing soil water potential of separation that repair ability and cell membrane lose repair ability realizes.
4. can represent according to claim 1. plant cell membrane degree of injury that the intensity of variation of cell leakage is selected the plant vigor analyzer (patent No.: ZL93247854.9) quantitative measurement for use with cell leakage.Estimate the method for the drought-resistance ability of plant by measuring plant drought stress killing point.
5. according to claim 1., this technology is applicable to arbor, shrub and draft.
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CN 200310113317 CN1616964A (en) | 2003-11-10 | 2003-11-10 | Quantitatice evaluation technology for plant drought tolerance |
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CN 200310113317 CN1616964A (en) | 2003-11-10 | 2003-11-10 | Quantitatice evaluation technology for plant drought tolerance |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101221159B (en) * | 2007-12-04 | 2012-09-05 | 上海市农业生物基因中心 | Drought resistance appraising method |
CN103518500A (en) * | 2012-07-04 | 2014-01-22 | 长江大学 | Method for detecting drought resistance of granular seeds in seedling stage |
CN103650948A (en) * | 2013-12-18 | 2014-03-26 | 东北农业大学 | Identification method for soybean drought resistance |
CN104112067A (en) * | 2014-07-02 | 2014-10-22 | 中国水利水电科学研究院 | Drought loss model building method |
CN109105091A (en) * | 2018-09-12 | 2019-01-01 | 中国林业科学研究院林业新技术研究所 | A kind of rapid assay methods of the tree species drought resistance based on stomata margin of safety index |
CN109633092A (en) * | 2018-12-25 | 2019-04-16 | 北京林业大学 | A method of detection poplar drought stress critical value |
-
2003
- 2003-11-10 CN CN 200310113317 patent/CN1616964A/en active Pending
Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101221159B (en) * | 2007-12-04 | 2012-09-05 | 上海市农业生物基因中心 | Drought resistance appraising method |
CN103518500A (en) * | 2012-07-04 | 2014-01-22 | 长江大学 | Method for detecting drought resistance of granular seeds in seedling stage |
CN103650948A (en) * | 2013-12-18 | 2014-03-26 | 东北农业大学 | Identification method for soybean drought resistance |
CN104112067A (en) * | 2014-07-02 | 2014-10-22 | 中国水利水电科学研究院 | Drought loss model building method |
CN104112067B (en) * | 2014-07-02 | 2017-09-26 | 中国水利水电科学研究院 | A kind of arid loss model construction method |
CN109105091A (en) * | 2018-09-12 | 2019-01-01 | 中国林业科学研究院林业新技术研究所 | A kind of rapid assay methods of the tree species drought resistance based on stomata margin of safety index |
CN109105091B (en) * | 2018-09-12 | 2020-07-31 | 中国林业科学研究院林业新技术研究所 | Rapid determination method for drought resistance of tree species based on stomatal safety margin index |
CN109633092A (en) * | 2018-12-25 | 2019-04-16 | 北京林业大学 | A method of detection poplar drought stress critical value |
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