CN109105091A - A kind of rapid assay methods of the tree species drought resistance based on stomata margin of safety index - Google Patents
A kind of rapid assay methods of the tree species drought resistance based on stomata margin of safety index Download PDFInfo
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- 238000003556 assay Methods 0.000 title claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 105
- 208000005189 Embolism Diseases 0.000 claims abstract description 49
- 238000005259 measurement Methods 0.000 claims abstract description 17
- 230000000243 photosynthetic effect Effects 0.000 claims description 12
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 3
- 231100000518 lethal Toxicity 0.000 claims description 3
- 230000001665 lethal effect Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- ATJFFYVFTNAWJD-UHFFFAOYSA-N Tin Chemical compound [Sn] ATJFFYVFTNAWJD-UHFFFAOYSA-N 0.000 claims 1
- 238000000691 measurement method Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 25
- 230000007613 environmental effect Effects 0.000 abstract description 4
- 241000894007 species Species 0.000 description 85
- 238000000034 method Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 235000019738 Limestone Nutrition 0.000 description 3
- 238000003066 decision tree Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 239000006028 limestone Substances 0.000 description 3
- 230000006978 adaptation Effects 0.000 description 2
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- 241001093951 Ailanthus altissima Species 0.000 description 1
- 241000169680 Aphloia theiformis Species 0.000 description 1
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- 235000011511 Diospyros Nutrition 0.000 description 1
- 244000236655 Diospyros kaki Species 0.000 description 1
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- 235000003333 Diospyros lotus Nutrition 0.000 description 1
- 240000008375 Hymenaea courbaril Species 0.000 description 1
- 241000279690 Ixora pavetta Species 0.000 description 1
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- 235000009496 Juglans regia Nutrition 0.000 description 1
- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 description 1
- 240000000249 Morus alba Species 0.000 description 1
- 235000008708 Morus alba Nutrition 0.000 description 1
- 240000000432 Pistacia chinensis Species 0.000 description 1
- 235000014123 Pistacia chinensis Nutrition 0.000 description 1
- 235000002413 Prunus ansu Nutrition 0.000 description 1
- 241000422846 Sequoiadendron giganteum Species 0.000 description 1
- 102000019197 Superoxide Dismutase Human genes 0.000 description 1
- 108010012715 Superoxide dismutase Proteins 0.000 description 1
- 244000089698 Zanthoxylum simulans Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000012174 chinese wax Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
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- 230000008641 drought stress Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Forests & Forestry (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention discloses a kind of rapid assay methods of tree species drought resistance based on stomata margin of safety index.The rapid assay methods include: that stomata closes the determination step of water potential value and the determination step of sapwood embolism threshold value water potential value；Wherein, it is the stomata margin of safety that the stomata, which closes the difference of water potential value and embolism threshold value water potential value, the stomata safe edge actual value of tree species then corresponding strong drought resistance greatly.Index of the present invention using the stomata margin of safety as measurement tree species drought resistance, the stomata margin of safety is to reflect the character of plant inherent attribute, big variation will not occur with environmental change, enable to tree species drought resistance measurement result more accurate, it can more fully reflect that tree species stomata closes the overall characteristic of water conservation and anti-sapwood embolism, and with xylem embolism resistance ability and the waterpower margin of safety compared with the correlation of tree species survival rate under drought condition, the stomata margin of safety and the correlation of tree species survival rate are higher.
Description
Technical field
The present invention relates to the technical fields of tree species drought resistance measurement, are based on stomata margin of safety index more particularly to one kind
Tree species drought resistance rapid assay methods.
Background technique
Arid is to limit the main environment factor of trees survival and growth, response and adaptation mechanism of the plant to drought stress
The always primary study content of plant physioecology.As drought event caused by climate change takes place frequently, all parts of the world is gloomy
Woods, which decays, dead significantly increases.And the good soil of condition has been afforested, and remaining is mostly the Difficult site of drought and barren.Dry
The barren Difficult site of drought is afforested, and is to select the reproducting tree species of suitable drought resistance, but it is difficult to afforest for future first
Big Difficult site is spent, how accurately, scientifically the tree species of selection and appropriate mix still have various problems.Sentence so grasping
The method of drought resistance power is that different Difficult sites selects suitable tree species to be very important between disconnected tree species.
Currently, most can real proof tree inter-species drought resistance method, one piece of typical Difficult site is exactly selected, needs
Its survival rate is investigated in the tree species plantation compared thereon, after the several years, carrys out decision tree inter-species drought resistance with the height of survival rate.But
The time that this method needs to grow very much at least needs 5 years or more.Come so finding a kind of more easy and reliable index
The drought resistance characterized between tree species is very necessary.
The embolism resistance ability of xylem is considered as a kind of important Drought Resistance of Trees index, and embolism resistance ability is stronger, tree
Wood can more maintain the intracorporal water conduction of trees under drought condition.But this is the one aspect of trees reply arid, institute
Can not comprehensively, accurately judge tree species drought resistance.
In research before, the waterpower margin of safety is considered as the index of extraordinary prediction tree species drought resistance.Waterpower
The margin of safety is the minimum flow of water of tree species and the difference of sapwood embolism threshold value water potential value.But the flow of water of tree species is with environment
Variation and change, if it is desired to accurately obtain tree species the minimum flow of water, then need to monitor longer time, usually one growth
Season to several years, so the waterpower margin of safety index of tree species is not the numerical value determined very much or needs the long period could
Accurate Determining, that is to say, that the waterpower margin of safety has the uncertain and minute period long.
The biochemical indicators such as superoxide dismutase, malonaldehyde also are commonly used to characterize tree species drought resistance in the prior art.This
A little biochemical indicators can be responded with drought condition variation, but single biochemical indicator is difficult directly to represent drought resisting between tree species
Property, and this kind of biochemical indicator can change with environmental change, can not trees quantification drought resistance to normal growth.Separately
Outside, if Stress treatment is taken to measure these biochemical indicators, need the long period, will lead to when tree species are more heavy workload and
Accuracy is low, and the drought resistance of seedling is also difficult to represent adult tree species.
To sum up, a kind of method is needed to be capable of the power of quickly and accurately decision tree inter-species drought resistance at present.
Summary of the invention
The purpose of the present invention is to provide a kind of quick measurement sides of tree species drought resistance based on stomata margin of safety index
Method, be able to solve present in the measurement of prior art tree species drought resistance that minute is long, determination data is affected by environment big and
The problem of measurement result inaccuracy.
To achieve the above object, the present invention provides a kind of the quick of tree species drought resistance based on stomata margin of safety index
Measuring method, comprising: stomata closes the determination step of water potential value and the determination step of sapwood embolism threshold value water potential value；Wherein,
It is the stomata margin of safety, the stomata safe edge of tree species that the stomata, which closes water potential value and the difference of embolism threshold value water potential value,
Actual value then corresponding strong drought resistance greatly；
Wherein, the determination step of the stomata closing water potential value includes:
Step A: after rain or after water pours, the branch of tree species to be determined is acquired in the fine day morning；
Step B: in the sun, using the stomatal conductance of portable photosynthetic instrument measurement blade；
Step C: after having surveyed stomatal conductance, cutting the blade of branch at once, using the water of pressure chamber's water potential meter measurement blade
Gesture；A piece of blade surveys the stomatal conductance and water potential value of another blade again at once after having surveyed, obtain the stomata under different water potential gradients
Degree of leading, until stomatal conductance is close to zero；
Step D: it is that ordinate is mapped using the flow of water of blade as the stomatal conductance of abscissa, blade, obtains blade
Stomatal conductance-flow of water relation curve, on the stomatal conductance-flow of water relation curve, when closing 88% with maximum stomatal conductance
Point as stomata close water potential value；
The determination step of the sapwood embolism threshold value water potential value includes:
Step E: morning acquires the branch of tree species to be determined after selection rain, and taking back interior after branch acquisition at once makes branch certainly
Right dehydration, and the sprig on blade or big bifurcated is wrapped up with tinfoil paper in branch upper and lower part；
Step F: when dehydration to a certain extent when, be protected from light, make the water balance of entire branch 2 hours；Later
The flow of water of above-mentioned blade or sprig is measured with pressure chamber's water potential meter；Then, long branch is immersed in the water at once cut measurement it is wooden
The stem section of portion's embolism, and neat stem section notch is repaired with sharp blade, then sapwood embolism, measurement are measured with low pressure liquid fluid system
More branches；It is ordinate by abscissa, embolism value of the flow of water, constructs Vulnerability of Xylem Embolism curve, broad leaf tree embolism
The flow of water (P88) when 88% is the lethal flow of water, using the water potential value as embolism threshold value water potential value.
In a preferred embodiment, in step A, acquire the selection of time of branch after the rain fine day morning 9:00 it
Afterwards.
In a preferred embodiment, in step B, the portable photosynthetic instrument is the photosynthetic instrument of Li-6400XT.
In a preferred embodiment, in step B, leaf chamber light source is set as 1500 μm of ol/ in the portable photosynthetic instrument
m2·s。
Wherein, leaf chamber light source is set as 1500 μm of ol/m in above-mentioned portable photosynthetic instrument2S, it is comparable between tree species to guarantee
Property.
In a preferred embodiment, in step C, pressure chamber's water potential meter is the pressure chamber PMS water potential meter.
In a preferred embodiment, in step E, the branch length selected when acquiring branch, which is greater than the tree species maximum, is led
1.5 times of length of tube.
Wherein, the branch length of selection, which is greater than 1.5 times of the tree species maximum catheter length, to generate people to avoid when cutting
For embolism.
In a preferred embodiment, in step F, each tree species at least measure 15 branches.
In a preferred embodiment, it in step F, is protected from light using the fully wrapped around branch of black polybag.
Compared with prior art, the invention has the following beneficial effects:
(1) index of the present invention using the stomata margin of safety as measurement tree species drought resistance, the big tree of the stomata margin of safety
Its drought resistance of kind is stronger, and the stomata margin of safety is to reflect the character of plant inherent attribute, can be with quantification, will not be with environmental change
And big variation occurs, therefore, the index using the stomata margin of safety as measurement tree species drought resistance enables to tree species drought resisting
Property measurement result is more accurate.
(2) under drought condition, trees are to close stomata there are two types of main adaptation arid strategy, one, reduce moisture
It scatters and disappears, the other is resisting xylem vessel's embolism of transporting moisture；Stomata margin of safety index has unified plant in the present invention
Stomata regulating strategy and anti-sapwood embolism strategy, represent the cooperative ability of tree species Stomatal morphology and xylem embolism resistance, compare
Tree species drought resistance is only determined in terms of xylem embolism resistance ability in existing, can more fully reflect that tree species stomata closes water conservation
With the overall characteristic of anti-sapwood embolism.
(3) xylem embolism resistance ability and the waterpower margin of safety are in really correlation with tree species survival rate, but stomata is pacified
Full limit is higher compared with the two with the correlation of tree species survival rate, closes water potential value from there through stomata, embolism resistance ability refers to
Mark, waterpower margin of safety index, the stomata margin of safety and the correlation of survival rate are strong and weak, illustrate that the stomata margin of safety is decision tree
The best index of kind drought resistance.
(4) present invention is short using the time used in stomata margin of safety index determining tree species drought resistance, the stomata of a tree species
It can measure and finish in the margin of safety several days, and existing waterpower margin of safety index needed to measure a Growing season by several years
Time, measuring method detection cycle of the present invention are shorter.
Detailed description of the invention
Fig. 1 is different tree species stomata margin of safety index-survival rate relational graph according to an embodiment of the present invention.
Fig. 2 is that the different tree species Stoma of Leaves of comparative example according to the present invention closes flow of water point-survival rate relational graph.
Fig. 3 is different tree species xylem embolism resistance ability-survival rate relational graph of comparative example according to the present invention.
Fig. 4 is the different tree species xylem waterpower margin of safety-survival rate relational graph of comparative example according to the present invention.
Specific embodiment
With reference to the accompanying drawing, specific embodiments of the present invention will be described in detail, it is to be understood that guarantor of the invention
Shield range is not limited by the specific implementation.
Unless otherwise explicitly stated, otherwise in entire disclosure and claims, term " includes " or its change
Changing such as "comprising" or " including " etc. will be understood to comprise stated element or component, and not exclude other members
Part or other component parts.
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Portable photosynthetic instrument is purchased from Beijing Li Gaotai Science and Technology Ltd., model Li-6400XT.
Pressure chamber's water potential meter is purchased from Ore. PMS Instrument Ltd., model PMS 1000.
A kind of embodiment: rapid assay methods of the above-mentioned tree species drought resistance based on stomata margin of safety index
(1) sample plot is summarized:
Carrying out field afforestation experiment with the representative Limestone Mountain in North China, place is located at Shandong Province Tai'an,
Naked rock ratio > 40% of this Limestone Mountain, soil thickness 5-15cm, great drought in North China infertile mountainous area feature.
(2) tree species are tested:
Selection 2011 in above-mentioned 15 experimental field planted tree species, this 15 tree species be respectively Chinese wax, tree-of-heaven, locust tree,
Walnut, Chinese prickly ash, Chinese pistache, smoke tree, torch tree, the fruit of date plum, China tree, Luan Shu, mulberry tree, ansu apricot, persimmon, mountain peach.These tree species are contained
North China planting out commonly most of drought resisting indigenous tree is covered, drought resistance is all very strong, but these trees kind are this serious
After surviving 7 years in the Limestone habitat of arid, the survival rate of different tree species still shows significant difference gradient.
The survival rate of tree species can represent the true drought resistance of tree species, therefore the height of tree species survival rate represents its drought resisting
The height of property.
(3) experimental procedure:
Fine day morning 10:00 acquires the branch of above-mentioned 15 kinds of tree species after rain；In the sun, photosynthetic using Li-6400XT
Instrument measures the stomatal conductance of blade, and leaf chamber light source is set as 1500 μm of ol/m in photosynthetic instrument2·s；After having surveyed stomatal conductance, cut at once
The blade of lower branch, using the flow of water of the pressure chamber PMS water potential meter measurement blade；A piece of blade surveys another blade after having surveyed again at once
Stomatal conductance and water potential value, the stomatal conductance under different water potential gradients is obtained, until stomatal conductance is close to zero；With blade
The flow of water be abscissa, blade stomatal conductance be that ordinate is mapped, obtain stomatal conductance-flow of water relation curve of blade
Figure, on the stomatal conductance-flow of water relation curve, point when closing 88% using maximum stomatal conductance closes the flow of water as stomata
Value；
The branch that morning gathers tree seeds after rain, the branch length of acquisition are greater than 1.5 times of this tree species maximum catheter length
Artificial embolism is generated to avoid when cutting；Taking back interior at once after branch acquisition makes branch nature dehydration, when dehydration to certain journey
It when spending, is protected from light using the fully wrapped around branch of black polybag, makes the water balance of entire branch 2 hours；It is pressed using PMS
Power room water potential meter measures the flow of water of above-mentioned package blade or sprig；Then, at once long branch is immersed in the water to cut and needs to measure
The stem section of sapwood embolism, and neat stem section notch is repaired with sharp blade, sapwood embolism then is measured with low pressure liquid fluid system,
15 branches are measured, are ordinate by abscissa, embolism value of the flow of water, construct Vulnerability of Xylem Embolism curve, broad leaf tree bolt
The flow of water (P88) when filling in 88% is the lethal flow of water, using the water potential value as embolism threshold value water potential value；
It is the stomata margin of safety that stomata, which closes water potential value and the difference of embolism threshold value water potential value,；Referred to the stomata margin of safety
It is denoted as abscissa, the survival rate of corresponding tree species obtains the margin of safety of different tree species stomata shown in Fig. 1 index-as ordinate
Survival rate relational graph, different points represents different tree species in figure.
On the mountain of arid, the corresponding drought resistance of the high tree species of survival rate is high, therefore, represents tree species with the height of survival rate
Drought resistance size.As shown in Figure 1, survival rate is significant related to the stomata margin of safety between tree species, and related coefficient (R) reaches
0.782, higher than the related coefficient of other indexs in comparative example, show using drought resistance between stomata margin of safety index characterization tree species
It is more able to reflect the true drought resistance of tree species, and using drought resistance between stomata margin of safety Indexs measure tree species by environmental disturbances
It is small, minute is short.
Comparative example: water potential value, embolism resistance capacity index, waterpower margin of safety index are closed using the stomata of tree species and surveyed respectively
Determine drought resistance
(1) drought resistance that flow of water point measures above-mentioned 15 tree species is closed based on stomata
Fine day morning 10:00 acquires the branch of above-mentioned 15 tree species after rain；In the sun, photosynthetic using Li-6400XT
Instrument measures the stomatal conductance of blade, and leaf chamber light source is set as 1500 μm of ol/m in photosynthetic instrument2·s；After having surveyed stomatal conductance, cut at once
The blade of lower branch, using the flow of water of the pressure chamber PMS water potential meter measurement blade；A piece of blade surveys another blade after having surveyed again at once
Stomatal conductance and water potential value, the stomatal conductance under different water potential gradients is obtained, until stomatal conductance is close to zero；With blade
The flow of water be abscissa, blade stomatal conductance be that ordinate is mapped, obtain stomatal conductance-flow of water relation curve of blade
Scheme (referring to fig. 2), on stomatal conductance-flow of water relation curve, point when closing 88% using maximum stomatal conductance is closed as stomata
Close water potential value；Flow of water index is closed as abscissa using stomata, and the survival rate of corresponding tree species obtains shown in Fig. 2 as ordinate
Different tree species stomata closes flow of water point-survival rate relational graph, and different points represents different tree species in figure.
As shown in Figure 2, stomata, which closes flow of water point, does not have correlation with survival rate, i.e. whether tree species stomata easily closes, can not
Represent the drought resistance of tree species.
(2) drought resistance of above-mentioned 15 tree species is measured based on xylem embolism resistance capacity index
Morning acquires the branch of above-mentioned 15 kinds of tree species after rain, and the branch length of acquisition is greater than this tree species maximum catheter length
1.5 times；Taking back interior makes branch nature dehydration, when dehydration to a certain extent when, using the fully wrapped around branch of black polybag into
Row is protected from light processing, makes the water balance of entire branch 2 hours；Above-mentioned package blade or small is measured using the pressure chamber PMS water potential meter
The flow of water of branch；Then, long branch is immersed in the water at once the stem section cut and need to measure sapwood embolism, and with sharp blade
Neat stem section notch is repaired, the sapwood embolism of 15 branches is then measured with low pressure liquid fluid system, using the flow of water as abscissa, embolism value
For ordinate, Vulnerability of Xylem Embolism curve is constructed, the flow of water (P88) when broad leaf tree embolism 88% is the lethal flow of water, by this
Water potential value is as embolism threshold value water potential value；Using embolism resistance capacity index as abscissa, the survival rate of corresponding tree species is sat as vertical
Mark, obtains the embolism resistance of different tree species xylem shown in Fig. 3 ability-survival rate relational graph, and different points represents different trees in figure
Kind.
From the figure 3, it may be seen that the embolism resistance ability of xylem is significant related to survival rate, but coefficient R is 0.673, small
Related coefficient (R=0.782) in embodiment.
(3) drought resistance based on above-mentioned 15 tree species of waterpower margin of safety index determining
The waterpower margin of safety is the season minimum flow of water of tree species and the difference of sapwood embolism threshold value water potential value.Wherein, wooden
The determination step of matter portion embolism threshold value water potential value is as described above.The season rapid assay methods of the minimum flow of water are: long in 1 year
When phase does not rain, repeatedly with the flow of water of pressure chamber's water potential meter measurement tree species, the season of the minimums of these water potential values tree species thus is taken
Save the minimum flow of water.The season minimum flow of water of the above-mentioned 15 kinds of tree species measured is made the difference with sapwood embolism threshold value water potential value, is obtained
Waterpower safe edge actual value；Using waterpower margin of safety index as abscissa, the survival rate of corresponding tree species obtains figure as ordinate
The waterpower of different tree species xylem shown in 4 margin of safety-survival rate relational graph, different points represents different tree species in figure.
As shown in Figure 4, the waterpower margin of safety is also significant related to survival rate, but coefficient R is 0.73, is less than and implements
Related coefficient (R=0.782) in example.
In short, xylem embolism resistance ability and the waterpower margin of safety are in really correlation with tree species survival rate, but stomata
The margin of safety is higher compared with the two with the correlation of tree species survival rate, closes water potential value, embolism resistance ability from there through stomata
Index, waterpower margin of safety index, the stomata margin of safety and the correlation of survival rate are strong and weak, illustrate that the stomata margin of safety is judgement
The best index of tree species drought resistance.
The aforementioned description to specific exemplary embodiment of the invention is in order to illustrate and illustration purpose.These descriptions
It is not wishing to limit the invention to disclosed precise forms, and it will be apparent that according to the above instruction, can much be changed
And variation.The purpose of selecting and describing the exemplary embodiment is that explaining specific principle of the invention and its actually answering
With so that those skilled in the art can be realized and utilize a variety of different exemplary implementation schemes of the invention and
Various chooses and changes.The scope of the present invention is intended to be limited by claims and its equivalents.
Claims (8)
1. a kind of rapid assay methods of the tree species drought resistance based on stomata margin of safety index, it is characterised in that: the measurement
Method includes: that stomata closes the determination step of water potential value and the determination step of sapwood embolism threshold value water potential value；Wherein, the gas
The difference of bore closure water potential value and embolism threshold value water potential value is the stomata margin of safety, and the stomata safe edge actual value of tree species is big
Then corresponding strong drought resistance；
Wherein, the determination step of the stomata closing water potential value includes:
Step A: after rain or after water pours, the branch of tree species to be determined is acquired in the fine day morning；
Step B: in the sun, using the stomatal conductance of portable photosynthetic instrument measurement blade；
Step C: after having surveyed stomatal conductance, cutting the blade of branch at once, using the flow of water of pressure chamber's water potential meter measurement blade；One
Piece blade surveys the stomatal conductance and water potential value of another blade again at once after having surveyed, obtain the stomatal conductance under different water potential gradients,
Until stomatal conductance is close to zero；
Step D: it is that ordinate is mapped using the flow of water of blade as the stomatal conductance of abscissa, blade, obtains the stomata of blade
Degree of leading-flow of water relation curve, the point on the stomatal conductance-flow of water relation curve, when with maximum stomatal conductance closing 88%
Water potential value is closed as stomata；
The determination step of the sapwood embolism threshold value water potential value includes:
Step E: morning acquires the branch of tree species to be determined after selection rain, and taking back interior at once after branch acquisition loses branch naturally
Water, and the sprig on blade or big bifurcated is wrapped up with tinfoil paper in branch upper and lower part；
Step F: when dehydration to a certain extent when, be protected from light, make the water balance of entire branch 2 hours；Later with pressure
Power room water potential meter measures the flow of water of above-mentioned blade or sprig；Then, long branch is immersed in the water at once and cuts measurement xylem bolt
The stem section of plug, and neat stem section notch is repaired with sharp blade, sapwood embolism then is measured with low pressure liquid fluid system, measures more
Branch；It is ordinate by abscissa, embolism value of the flow of water, Vulnerability of Xylem Embolism curve is constructed, when broad leaf tree embolism 88%
The flow of water (P88) be the lethal flow of water, using the water potential value as embolism threshold value water potential value.
2. rapid assay methods according to claim 1, which is characterized in that in step A, the selection of time for acquiring branch exists
After rain after fine day morning 9:00.
3. rapid assay methods according to claim 1, which is characterized in that in step B, the portable photosynthetic instrument is
The photosynthetic instrument of Li-6400XT.
4. rapid assay methods according to claim 1 or 3, which is characterized in that in step B, the portable photosynthetic instrument
Middle leaf chamber light source is set as 1500 μm of ol/m2·s。
5. rapid assay methods according to claim 1, which is characterized in that in step C, pressure chamber's water potential meter is
The pressure chamber PMS water potential meter.
6. rapid assay methods according to claim 1, which is characterized in that in step E, the branch that selects when acquiring branch
Length is greater than 1.5 times of the tree species maximum catheter length.
7. rapid assay methods according to claim 1, which is characterized in that in step F, each tree species at least measure 15
Branch.
8. rapid assay methods according to claim 1, which is characterized in that fully wrapped around using black polybag in step F
Branch is protected from light.
Priority Applications (1)
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113203655A (en) * | 2021-05-24 | 2021-08-03 | 昆明理工大学 | Measuring equipment for measuring anti-embolism degree of plant xylem by using pneumatic method |
| CN113466289A (en) * | 2021-06-28 | 2021-10-01 | 中国农业大学 | System and method for measuring vulnerability of crop leaf embolism |
| CN114383666A (en) * | 2022-01-20 | 2022-04-22 | 信阳师范学院 | Leaf hydraulic vulnerability curve measuring equipment and method |
| CN114441712A (en) * | 2022-01-21 | 2022-05-06 | 中国农业大学 | Irrigation curve determination method and device based on air hole fragility characteristics |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616964A (en) * | 2003-11-10 | 2005-05-18 | 尹伟伦 | Quantitatice evaluation technology for plant drought tolerance |
| CN101221159A (en) * | 2007-12-04 | 2008-07-16 | 上海市农业生物基因中心 | Drought resistance appraising method |
| CN103238493A (en) * | 2013-05-20 | 2013-08-14 | 江苏省农业科学院 | Responses of different peach stock types for persistent drought and evaluation method of drought resistance of different peach stock types |
| CN103270871A (en) * | 2013-05-16 | 2013-09-04 | 中国农业科学院兰州畜牧与兽药研究所 | Method for fast detecting drought resistance of alfalfa varieties and sorting drought-resistant alfalfa varieties |
| CN103630656A (en) * | 2013-12-18 | 2014-03-12 | 江苏大学 | Method for quantitatively detecting drought resistance of plants |
| CN104007093A (en) * | 2014-05-22 | 2014-08-27 | 江苏大学 | Method for rapidly and quantitatively calculating inherent drought resistance of plants |
-
2018
- 2018-09-12 CN CN201811061623.4A patent/CN109105091B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1616964A (en) * | 2003-11-10 | 2005-05-18 | 尹伟伦 | Quantitatice evaluation technology for plant drought tolerance |
| CN101221159A (en) * | 2007-12-04 | 2008-07-16 | 上海市农业生物基因中心 | Drought resistance appraising method |
| CN103270871A (en) * | 2013-05-16 | 2013-09-04 | 中国农业科学院兰州畜牧与兽药研究所 | Method for fast detecting drought resistance of alfalfa varieties and sorting drought-resistant alfalfa varieties |
| CN103238493A (en) * | 2013-05-20 | 2013-08-14 | 江苏省农业科学院 | Responses of different peach stock types for persistent drought and evaluation method of drought resistance of different peach stock types |
| CN103630656A (en) * | 2013-12-18 | 2014-03-12 | 江苏大学 | Method for quantitatively detecting drought resistance of plants |
| CN104007093A (en) * | 2014-05-22 | 2014-08-27 | 江苏大学 | Method for rapidly and quantitatively calculating inherent drought resistance of plants |
Non-Patent Citations (2)
| Title |
|---|
| 李燕 等: "树木抗旱机理研究进展", 《生态学杂志》 * |
| 陈志成 等: "木本植物木质部栓塞测定技术的争议与进展", 《林业学报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113203655A (en) * | 2021-05-24 | 2021-08-03 | 昆明理工大学 | Measuring equipment for measuring anti-embolism degree of plant xylem by using pneumatic method |
| CN113466289A (en) * | 2021-06-28 | 2021-10-01 | 中国农业大学 | System and method for measuring vulnerability of crop leaf embolism |
| CN114383666A (en) * | 2022-01-20 | 2022-04-22 | 信阳师范学院 | Leaf hydraulic vulnerability curve measuring equipment and method |
| CN114383666B (en) * | 2022-01-20 | 2023-11-28 | 信阳师范学院 | Leaf hydraulic vulnerability curve measuring equipment and method |
| CN114441712A (en) * | 2022-01-21 | 2022-05-06 | 中国农业大学 | Irrigation curve determination method and device based on air hole fragility characteristics |
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