CN109452099B - Method for screening drought-enduring flax variety - Google Patents
Method for screening drought-enduring flax variety Download PDFInfo
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- CN109452099B CN109452099B CN201811610000.8A CN201811610000A CN109452099B CN 109452099 B CN109452099 B CN 109452099B CN 201811610000 A CN201811610000 A CN 201811610000A CN 109452099 B CN109452099 B CN 109452099B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/30—Growth substrates; Culture media; Apparatus or methods therefor based on or containing synthetic organic compounds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
Abstract
The invention discloses a method for screening drought-enduring flax varieties, which comprises the following steps: selecting and disinfecting the flaxseed which is full, uniform and disease-free, and then sowing the flaxseed in a cultivation groove, and covering the flaxseed with a substrate after sowing; dividing the obtained flax seedlings into a drought treatment group, a control 1 group and a control 2 group; the drought treatment group adopts organic ecological culture medium for planting, the drought treatment is started after 5 days of thinning, the relative humidity of the medium is reduced to 30 percent in a stepped mode, and the drought is maintained; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%; respectively counting the underground dry weight of each flax of the drought treatment group, the control 1 group and the control 2 group; and calculating the drought resistance value of each flax variety. The method has the advantages of low cost, high drought tolerance detection accuracy, short detection period, easy operation and convenient popularization and implementation in various places.
Description
Technical Field
The invention relates to the technical field of plant variety screening, in particular to a method for screening drought-enduring flax varieties.
Background
Flax is one of the important oil crops in China, and besides being rich in polyunsaturated fatty acid-alpha-linolenic acid which is necessary for human bodies, the flax seeds also contain other important active ingredients, such as secoisolariciresinol diglucoside, dietary fibers and the like. The lignan content in the flax seeds is about 800 times of that of other common crops, and the flax lignan has the effects of resisting tumor, resisting osteoporosis, relieving climacteric symptoms and the like. The semen Lini is rich in soluble plant cellulose, and has cholesterol reducing effect. The frequent eating of the linseed can reduce the incidence of diseases such as constipation, obesity, heart disease and the like. The flax is mainly distributed in provinces such as Shanxi, Gansu, Ningxia and inner Mongolia in China, and drought stress often exists in arid seasons or arid and semi-arid regions, so that the production of the flax is seriously influenced. Therefore, improving the drought resistance of crop varieties has become a major topic of agricultural research in arid and semi-arid regions; and accurately combines the drought resistance of crop varieties, and is a necessary foundation for cultivating drought-resistant varieties. The drought resistance identification is the process of screening, evaluating and classifying according to the drought resistance of crop varieties; high-quality germplasm can be provided for drought-resistant breeding through drought-resistant identification. Although different researchers do research work on drought resistance of the flax from various aspects and beneficial discussion is carried out for knowing the drought resistance of the flax, the screening of the existing drought-resistant flax variety has the defects of low accuracy and the like, and a method for cultivating the drought-resistant flax variety with easily controlled cultivation environment, easy operation and high accuracy is lacked.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides the method for screening drought-enduring flax varieties, which has the advantages of low cost, high drought-enduring detection accuracy, short detection period, easy operation and convenient popularization and implementation in various places.
In order to achieve the purpose, the technical scheme provided by the invention is as follows:
the invention provides a method for screening drought-enduring flax varieties, which comprises the following steps: s1, selecting and disinfecting the flawless and full, uniform and healthy flax seeds, sowing the seeds in a cultivation groove in a drilling mode, and covering the seeds with a substrate after sowing; thinning when the number of leaves reaches 5/6 after emergence, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate is controlled to be 75 percent; step S2, dividing the flax seedlings into a drought treatment group, a contrast 1 group and a contrast 2 group; the drought treatment group adopts organic ecological culture medium for planting, the drought treatment is started after 5 days of thinning, the relative humidity of the medium is reduced to 30 percent in a stepped manner, and the drought is maintained for a plurality of days; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%; step S3, respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, wherein the average value of the underground dry weights of 50 flax in a single cultivation tank is the underground dry weight of the flax of the cultivation tank, and the average value of the underground dry weights of the flax of a plurality of cultivation tanks corresponding to each group is the underground dry weight of the flax of the group; step S4, calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is; drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1); wherein, the dry relative underground part dry weight of the drought treatment group is equal to the dry underground part dry weight of the drought treatment group/the dry underground part dry weight of the contrast 2 group, and the dry relative underground part dry weight of the contrast 1 group is equal to the dry underground part dry weight of the contrast 1 group/the dry underground part dry weight of the contrast 2 group. It should be noted that, for the accuracy of drought resistance identification, when the drought resistance value of a single flax variety is calculated, a relative value is adopted so as to eliminate background interference caused by the culture medium.
Preferably, in step S1, the raw material components of the organic ecological culture medium include corn stalks, decomposed cow dung, rice hulls, bone residues, furnace slag and wormcast; the organic ecological culture medium is fermented and decomposed before use.
Further preferably, in step S1, the mass ratio of the corn stalks, the decomposed cow dung, the rice hulls, the bone residues, the furnace slag and the wormcast is 2: 4: 1: 1: 2: 5.
preferably, in step S1, carbendazim is added in an amount of 1g per 10L of the organic ecological culture medium.
Preferably, in step S1, the physicochemical properties of the organic ecological culture medium include: the volume weight is 0.75g/cm3The total porosity was 80%, the air vent pore was 18%, the water holding pore was 62%, the pH was 6.9, the conductivity was 3.36ms/cm, and the cation exchange capacity was 59 cmol/kg.
Preferably, the entire screening process is carried out in a smart greenhouse with a relative humidity of the greenhouse air maintained at 65% and a temperature of 20 ℃.
Preferably, in step S1, the cultivation tank has a length of 40cm, a width of 40cm and a depth of 30 cm.
Preferably, in step S1, the covering thickness of the substrate is 1.5-2.5 cm, preferably 2 cm.
Preferably, in step S2, the drought-treated group, the control 1 group and the control 2 group respectively include three cultivation tanks.
Preferably, in step S2, the number of days is 25 days.
The technical scheme provided by the invention has the following beneficial effects:
(1) for plants, the development degree of underground root systems determines the strength of drought resistance; drought resistance of different varieties of the same plant is identified according to the development degree of underground roots, but the development degree of the roots is greatly influenced by the matrix. The culture substrate is more suitable for the growth of plant roots than the soil, for example, the total porosity of the culture substrate is larger than that of the soil substrate. In order to create a good growing environment for the root system, a cultivation substrate suitable for the plant needs to be developed firstly. The invention determines the most suitable formula of the flax organic ecological culture medium by researching the influence of different formula mediums on the growth vigor, the plant photosynthetic index, the root system, the yield and the quality of the flax, and compared with field planting, the culture medium has the advantages of good plant growth vigor, improved yield and quality, reduced use of chemical fertilizers and pesticides, water conservation and fertilizer conservation.
(2) Research shows that the drought resistance of different flax varieties is closely related to the dry weight of the underground part; the method for screening drought-enduring flax varieties provided by the invention has the advantages of low cost, high drought-enduring detection accuracy, short detection period, easiness in operation and convenience in popularization and implementation in various places.
Additional aspects and advantages of the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Detailed Description
The technical solution of the present invention will be clearly and completely described below with reference to the embodiments of the present invention. The following examples are only for illustrating the technical solutions of the present invention more clearly, and therefore are only examples, and the protection scope of the present invention is not limited thereby.
The experimental procedures in the following examples are conventional unless otherwise specified. The test materials used in the following examples were purchased from a conventional store unless otherwise specified. In the quantitative tests in the following examples, three replicates were set, and the data are the mean or the mean ± standard deviation of the three replicates.
Examples
The embodiment provides a method for screening drought-enduring flax varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of greenhouse air is kept at 65%, the temperature is 20 ℃, and the screening specifically comprises the following steps:
step S1 (flax seeding): selecting and disinfecting the flaxseed seeds which are full, uniform and disease-free, then sowing the seeds in a cultivation groove with the length of 40cm, the width of 40cm and the depth of 30cm, planting the seeds in a drilling mode, and keeping reasonable plant spacing; covering 2cm with the substrate after sowing;
thinning when the number of leaves after seedling emergence reaches 5/6, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 4: 1: 1: 2: 5, adding 1g of carbendazim into every 10L of organic ecological culture medium, namely, crushed corn straws, decomposed cow dung, rice hulls, bone residues, furnace slag and wormcast, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use, and has the following physical and chemical properties: the volume weight is 0.75g/cm3The total porosity was 80%, the air vent pore was 18%, the water holding pore was 62%, the pH was 6.9, the conductivity was 3.36ms/cm, and the cation exchange capacity was 59 cmol/kg.
Step S2 (drought treatment): dividing the flax seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 flax seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, starting drought treatment 5 days after thinning, reducing the relative humidity of the medium to 30 percent in a stepped manner within a certain time, and maintaining the drought for 25 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, and calculating the average value for each flax three times to obtain the underground dry weight of the flax;
the average value of the dry weights of the underground parts of 50 flax plants in a single cultivation tank is the dry weight of the underground part of the flax plant in the cultivation tank, and the average value of the dry weights of the underground parts of the flax plants in three corresponding cultivation tanks in each group is the dry weight of the underground part of the flax plant in the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is;
drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1);
wherein, the dry weight of the relative underground part of the drought treatment group is equal to the dry weight of the underground part of the drought treatment group/the dry weight of the underground part of the contrast group 2;
control 1 group relative dry underground part weight is control 1 group dry underground part weight/control 2 group dry underground part weight.
Comparative example 1
The comparison example provides a method for screening drought-enduring flax varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 65%, the temperature is 20 ℃, and the screening specifically comprises the following steps:
step S1 (flax seeding): selecting and disinfecting the flaxseed seeds which are full, uniform and disease-free, then sowing the seeds in a cultivation groove with the length of 40cm, the width of 40cm and the depth of 30cm, planting the seeds in a drilling mode, and keeping reasonable plant spacing; covering 2cm with the substrate after sowing;
thinning when the number of leaves after seedling emergence reaches 5/6, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in percentage by mass of 4: 4: 1: 1: 2: 5, adding 1g of carbendazim into every 10L of organic ecological culture medium, namely, crushed corn straws, decomposed cow dung, rice hulls, bone residues, furnace slag and wormcast, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the flax seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 flax seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, starting drought treatment 5 days after thinning, reducing the relative humidity of the medium to 30 percent in a stepped manner within a certain time, and maintaining the drought for 25 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, and calculating the average value for each flax three times to obtain the underground dry weight of the flax;
the average value of the dry weights of the underground parts of 50 flax plants in a single cultivation tank is the dry weight of the underground part of the flax plant in the cultivation tank, and the average value of the dry weights of the underground parts of the flax plants in three corresponding cultivation tanks in each group is the dry weight of the underground part of the flax plant in the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is;
drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1);
wherein, the dry weight of the relative underground part of the drought treatment group is equal to the dry weight of the underground part of the drought treatment group/the dry weight of the underground part of the contrast group 2;
control 1 group relative dry underground part weight is control 1 group dry underground part weight/control 2 group dry underground part weight.
Comparative example 2
The comparison example provides a method for screening drought-enduring flax varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 65%, the temperature is 20 ℃, and the screening specifically comprises the following steps:
step S1 (flax seeding): selecting and disinfecting the flaxseed with full, uniform and consistent seeds and no disease, then sowing the seeds in a cultivation groove with the length of 100cm, the width of 50cm and the depth of 30cm, planting the seeds in a drilling mode, and keeping reasonable plant spacing; covering 2cm with the substrate after sowing;
thinning when the number of leaves after seedling emergence reaches 5/6, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in mass ratio of 2: 4: 1: 3: 5, adding 1g of carbendazim into every 10L of organic ecological culture medium, wherein the organic ecological culture medium is fermented and decomposed before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the flax seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 flax seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, starting drought treatment 5 days after thinning, reducing the relative humidity of the medium to 30 percent in a stepped manner within a certain time, and maintaining the drought for 25 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, and calculating the average value for each flax three times to obtain the underground dry weight of the flax;
the average value of the dry weights of the underground parts of 50 flax plants in a single cultivation tank is the dry weight of the underground part of the flax plant in the cultivation tank, and the average value of the dry weights of the underground parts of the flax plants in three corresponding cultivation tanks in each group is the dry weight of the underground part of the flax plant in the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is;
drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1);
wherein, the dry weight of the relative underground part of the drought treatment group is equal to the dry weight of the underground part of the drought treatment group/the dry weight of the underground part of the contrast group 2;
control 1 group relative dry underground part weight is control 1 group dry underground part weight/control 2 group dry underground part weight.
Comparative example 3
The comparison example provides a method for screening drought-enduring flax varieties, the whole screening process is carried out in an intelligent greenhouse, the relative humidity of the air in the greenhouse is kept at 65%, the temperature is 20 ℃, and the screening specifically comprises the following steps:
step S1 (flax seeding): selecting and disinfecting the flaxseed seeds which are full, uniform and disease-free, then sowing the seeds in a cultivation groove with the length of 40cm, the width of 40cm and the depth of 30cm, planting the seeds in a drilling mode, and keeping reasonable plant spacing; covering 2cm with the substrate after sowing;
thinning when the number of leaves after seedling emergence reaches 5/6, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove on the basis of keeping reasonable plant spacing; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate of all the cultivation tanks is controlled to be 75% from sowing to before drought treatment; the organic ecological culture medium comprises the following raw material components in percentage by mass of 4: 1: 1: 2: 5, adding 1g of carbendazim into every 10L of organic ecological culture medium of decomposed cow dung, rice hulls, bone residues, furnace slag and wormcast, and fermenting and decomposing the organic ecological culture medium before use; the organic ecological culture medium is not pressed and tamped intentionally when in use.
Step S2 (drought treatment): dividing the flax seedlings into a drought treatment group, a control 1 group and a control 2 group; each of the 3 cultivation tanks is processed, 50 flax seedlings are left in each tank after thinning:
drought treatment group (cultivation substrate drought treatment): planting by adopting an organic ecological culture medium, starting drought treatment 5 days after thinning, reducing the relative humidity of the medium to 30 percent in a stepped manner within a certain time, and maintaining the drought for 25 days;
control 1 group (normal water content of culture medium): planting by adopting an organic ecological culture medium, wherein the relative humidity of the medium is always maintained at 75%;
control 2 group (soil matrix water content normal): the soil matrix is adopted for planting, and the relative humidity of the matrix is always maintained at 75%.
Step S3 (data statistics): respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, and calculating the average value for each flax three times to obtain the underground dry weight of the flax;
the average value of the dry weights of the underground parts of 50 flax plants in a single cultivation tank is the dry weight of the underground part of the flax plant in the cultivation tank, and the average value of the dry weights of the underground parts of the flax plants in three corresponding cultivation tanks in each group is the dry weight of the underground part of the flax plant in the group.
Step S4 (drought resistance calculation and drought resistance identification): calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is;
drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1);
wherein, the dry weight of the relative underground part of the drought treatment group is equal to the dry weight of the underground part of the drought treatment group/the dry weight of the underground part of the contrast group 2;
control 1 group relative dry underground part weight is control 1 group dry underground part weight/control 2 group dry underground part weight.
The growth of the underground dry weight of flax obtained in the examples of the present invention and comparative examples 1 to 3 was observed and compared with the underground dry weight of flax obtained by a conventional screening method, and the specific results are shown in table 1 below.
TABLE 1 growth of the dry weight of the lower part of flax of different groups
It is to be noted that, unless otherwise specified, technical or scientific terms used herein shall have the ordinary meaning as understood by those skilled in the art to which the invention pertains. Unless specifically stated otherwise, the relative steps, numerical expressions, and values of the components and steps set forth in these embodiments do not limit the scope of the present invention. In all examples shown and described herein, unless otherwise specified, any particular value should be construed as merely illustrative, and not restrictive, and thus other examples of example embodiments may have different values.
In the description of the present invention, it is to be understood that the terms "first", "second" and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implying any number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the present invention, and they should be construed as being included in the following claims and description.
Claims (8)
1. A method for screening drought-enduring flax varieties is characterized by comprising the following steps:
s1, selecting and disinfecting the flawless and full, uniform and healthy flax seeds, sowing the seeds in a cultivation groove in a drilling mode, and covering the seeds with a substrate after sowing; thinning when the number of leaves reaches 5/6 after emergence, selecting healthy seedlings with consistent growth vigor, and reserving 50 flax seedlings in each cultivation groove; wherein the substrate is an organic ecological culture substrate or a soil substrate; the relative humidity of the substrate is controlled to be 75 percent;
step S2, dividing the flax seedlings into a drought treatment group, a contrast 1 group and a contrast 2 group; the drought treatment group adopts organic ecological culture medium for planting, the drought treatment is started after 5 days of thinning, the relative humidity of the medium is reduced to 30 percent in a stepped manner, and the drought is maintained for a plurality of days; the control group 1 adopts organic ecological culture medium for planting, and the relative humidity of the medium is always maintained at 75%; the control group 2 was planted with soil matrix, and the relative humidity of the matrix was always maintained at 75%;
step S3, respectively counting the underground dry weight of each flax of the drought treatment group, the contrast 1 group and the contrast 2 group, wherein the average value of the underground dry weights of 50 flax in a single cultivation tank is the underground dry weight of the flax of the cultivation tank, and the average value of the underground dry weights of the flax of a plurality of cultivation tanks corresponding to each group is the underground dry weight of the flax of the group;
step S4, calculating the drought resistance value of each flax variety, wherein the larger the drought resistance value is, the stronger the drought resistance of the corresponding flax variety is; drought resistance value of a single flax variety (average of the relative underground dry weight of the drought treatment group of the variety/the relative underground dry weight of the drought treatment group of all the identified varieties) × (relative underground dry weight of the drought treatment group of the variety/relative underground dry weight of the control group of the variety 1); wherein, the dry weight of the relative underground part of the drought treatment group is equal to the dry weight of the underground part of the drought treatment group/the dry weight of the underground part of the contrast group 2, and the dry weight of the relative underground part of the contrast group 1 is equal to the dry weight of the underground part of the contrast group 1/the dry weight of the underground part of the contrast group 2;
in the step S1, the raw material components of the organic ecological culture medium comprise corn straws, decomposed cow dung, rice hulls, bone residues, furnace slag and wormcast; the organic ecological culture medium is fermented and decomposed before use; the mass ratio of the corn straw, the decomposed cow dung, the rice hull, the bone residues, the furnace slag and the wormcast is 2: 4: 1: 1: 2: 5;
the physicochemical properties of the organic ecological culture medium comprise: the volume weight is 0.75g/cm3The total porosity was 80%, the air vent pore was 18%, the water holding pore was 62%, the pH was 6.9, the conductivity was 3.36ms/cm, and the cation exchange capacity was 59 cmol/kg.
2. The method for screening drought tolerant flax varieties according to claim 1 wherein:
in the step S1, 1g of carbendazim is added to every 10L of organic ecological culture medium.
3. The method for screening drought tolerant flax varieties according to claim 1 wherein:
the whole screening process was carried out in an intelligent greenhouse with a relative humidity of 65% in the greenhouse air and a temperature of 20 ℃.
4. The method for screening drought tolerant flax varieties according to claim 1 wherein:
in the step S1, the cultivation tank has a length of 40cm, a width of 40cm, and a depth of 30 cm.
5. The method for screening drought tolerant flax varieties according to claim 1 wherein:
in the step S1, the covering thickness of the substrate is 1.5-2.5 cm.
6. The method of screening for drought tolerant flax varieties of claim 5 wherein: in step S1, the substrate is covered to a thickness of 2 cm.
7. The method for screening drought tolerant flax varieties according to claim 1 wherein:
in step S2, the drought-treated group, the control 1 group, and the control 2 group include three cultivation tanks, respectively.
8. The method for screening drought tolerant flax varieties according to claim 1 wherein:
in the step S2, the number of days is 25 days.
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