CN109451915A - A method of improving Europe Lee percentage of seedgermination - Google Patents

A method of improving Europe Lee percentage of seedgermination Download PDF

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Publication number
CN109451915A
CN109451915A CN201910019125.1A CN201910019125A CN109451915A CN 109451915 A CN109451915 A CN 109451915A CN 201910019125 A CN201910019125 A CN 201910019125A CN 109451915 A CN109451915 A CN 109451915A
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China
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seed
europe lee
europe
refrigerator
days
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CN201910019125.1A
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CN109451915B (en
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刘建珍
肖啸
张立彬
武军凯
宋立琴
王海静
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Hebei Normal University of Science and Technology
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Hebei Normal University of Science and Technology
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting

Abstract

The invention discloses a kind of methods for improving Europe Lee percentage of seedgermination, using the mature wet seed of 2-3 intermittent warming Europe Lee of low temperature refrigerator.Include the following steps: to acquire full ripe Europe plum fruit, is deposited under tree and allows its natural after-ripening, remove pulp after 3 days, seed is cleaned, band hygroscopic water is loaded in Fibre Bag;Europe Lee's seed is placed in -10~-15 DEG C of refrigerator-freezer, after freezing processing 30 days or so, is powered off to refrigerator-freezer, is powered after 5 days, continues to previous temperature freezing processing 30 days, after repeating aforesaid operations 2-3 times, Europe Lee's seed is frozen in spare in refrigerator-freezer;When outdoor daily mean temperature reaches 6-10 DEG C, after taking out the Europe Lee's seed natural thaw handled well, seed is seeded in wet husky earth, 1 month, Europe Lee's seed can germinate unearthed.The present invention can promote Europe Lee's seed Rapid reversal suspend mode, germinate ahead of time, and this method can accomplish to allow Europe Lee germination in 5 months, and germination percentage is high, and germination is neat.

Description

A method of improving Europe Lee percentage of seedgermination
Technical field
The present invention relates to a kind of Europe Lee method for treating seeds, and in particular to a method of improve Europe Lee percentage of seedgermination.
Background technique
Europe Lee is the distinctive rare Fruit Tree Resources in China, is a kind of short raw shrub, be distributed widely in 13 provinces of northern China, City and autonomous region.Europe Lee plant dwarfing, cold-resistant, drought resisting, resistance to ridge be thin, saline-alkali tolerant.Europe plum fruit is full of nutrition, unique flavor, i.e., It can eat raw, and processable.Europe Lee has wide development prospect as a kind of emerging fruit tree.Currently, the main breeding side of Europe Lee Method has sowing, cuttage, tissue cultures, grafting etc. several.When carrying out seed propagation with Europe Lee's seed, need at seed Reason, otherwise, seed is difficult to sprout, and germination rate is 10% or less.In order to improve the germination rate of Europe Lee's seed, produce to Europe Lee's kind The processing of son mainly has the side of the chemistry such as growth regulatory substance processing, acid etching, Stratificated treatment, mechanical dejacketing processing, physics Method.Wherein, growth regulatory substance facture is broken if carrying out immersion treatment with growth regulatory substances such as GA3,6-BA, NAA The suspend mode of seed promotes its germination, handles the time 100 days or so.In operating process, it should be noted that locating later in low-temperature stratification Reason, and the concentration of time, Auto-regulator and the wet degree of seed early period handled influences the germination rate of seed.Acid etching Method after hydrochloric acid, dense sulfuric acid treatment mainly with keeping kind of shell thinning, an enhancing kind shell permeability, improves seed germination rate with this, operates In the process, needing to control the time of the amount of seed and the ratio of acid, acid etching, short processing time influences treatment effect, and The processing time is long, but Interior Seed tissue can be made by acid etching, easily damaged to Interior Seed structure, while in treatment process High heat can be discharged, embryo vigor is influenced;And concentrated hydrochloric acid, concentrated sulfuric acid acid etching influence greatly, therefore, in life people, environment Acid etching should be used with caution in production.Stratificated treatment is to release Europe Lee seed dormancy, promotes to sprout most common method, passes through low temperature A series of physiological acoustic signals occur for lamination, Interior Seed, effectively release embryo dormancy and kind shell suspend mode, promote sprouting for seed Hair;In operating process, it should be noted that cleaning and water content, the low temperature of lamination and the time, lamination of the time of seed infiltration, sand The control of the lot of essential factors such as middle gas permeability.The shortcomings that Stratificated treatment is that (1) low-temperature stratification effect is less desirable, general First Year Germination rate 30%;(2) condition of Stratificated treatment depends on natural low temperature, and very big, germination percentage is influenced by outside environmental elements It is unstable, there is the phenomenon that next year sprouting;(3) poor timing of Stratificated treatment usually makes Europe Lee germinateed in underground Slow-witted overlong time, plumule growth is too long, influences survival rate after planting;(4) it needs that it is suitable to control it using clean river sand Water content.Mechanical dejacketing processing is to carry out skin breaking to the seed that do not sprout after Stratificated treatment, and percentage of seedgermination is bright The aobvious germination percentage higher than not broken shell seed;In operating process, it should be noted that the dynamics of decladding, the integrality for planting skin, kernel it is intact Property.The shortcomings that mechanical dejacketing processing is that the suitable dynamics of (1) grasp decladding is difficult, general artificial bad grasp;(2) skin, kind are planted Benevolence has the phenomenon that by destroying more, influences the germination for handling seed;(3) a large amount of seed treatment investments is artificial more.
In short, the major defect of the above technology is longer the time required to being germination;Operating process trouble;And some processing are to kind The viability of son has an impact, therefore makes the reduction of its germination percentage.
Summary of the invention
To solve the above problems, can be shortened Europe Lee's kind the present invention provides a kind of method for improving Europe Lee percentage of seedgermination Germination rate is sprouted ahead of time and improved to the time of subprocessing.
To achieve the above object, the technical scheme adopted by the invention is as follows:
A method of Europe Lee percentage of seedgermination is improved, using the wet seed of low temperature refrigerator 2-3 intermittent warming Europe Lee maturation, It can promote Europe Lee's seed Rapid reversal suspend mode, germinate ahead of time, this method can accomplish to allow Europe Lee germination in 5 months, and And germination percentage is high, germination is neat;Specifically comprise the following steps:
S1, the collecting fruit after the plum fruit full maturity of Europe are deposited under tree and allow its natural after-ripening, pulp is removed after 3 days, will Seed is cleaned, and band hygroscopic water is loaded in Fibre Bag, spare;
S2, Europe Lee's seed of pack is placed in -10~-15 DEG C of refrigerator-freezer, it is disconnected to refrigerator-freezer after freezing processing 30 days or so Electricity is powered after 5 days, continues to previous temperature freezing processing 30 days;
S3, after repeating step S22-3 times, Europe Lee's seed is frozen in spare in 0~5 DEG C of refrigerator-freezer;
S4, when outdoor daily mean temperature reaches 6-10 DEG C, after taking out the Europe Lee's seed natural thaw handled well, by seed It is seeded in wet husky earth, 1 month or so, Europe Lee's seed can germinate unearthed.
The present invention, with regard to wet process, compared with conventional Stratificated treatment, saves airing seed, seed impregnates, with clean using seed The processes such as net river sand seed dressing.Intermittent warming is carried out using low temperature refrigerator.Compared with conventional Stratificated treatment, eliminate outdoor dig pit, Rake opens mound and checks the processes such as seed situation.It can sow at any time, germination is neat, and germination rate is high.The method processing is simple, operation side Just, condition is controllable, can be used for the mass propagation of Europe Lee.
The present invention does not use any growth regulator substance, chemical agent, environmentally friendly, and will not cause to endanger to human body Evil.
Operation of the present invention is simple, condition is controllable, and artificial input amount is small, and does not have any damage to Europe Lee's seed.
The present invention can make Europe Lee's seed germinate in a relatively short period of time, and germinating time is concentrated.
Processing method of the invention is versatile, is suitable for various Europe Lee seeds and carries out the production of Europe Lee seminal propagation Person.
Specific embodiment
The present invention is described in detail combined with specific embodiments below.Following embodiment will be helpful to the technology of this field Personnel further understand the present invention, but the invention is not limited in any way.It should be pointed out that the ordinary skill of this field For personnel, without departing from the inventive concept of the premise, various modifications and improvements can be made.These belong to the present invention Protection scope.
Embodiment
Part of in September, 2,013 3000 Europe Lee's seed collected is divided into equal 3 parts.First part of seed after acquisition with Wet sand mixing carries out Sand culture on the spot.Second part of seed is placed under tree after 2-3 days, and pulp rots completely, collects clean kind Son carries out low temperature refrigerator processing, and treatment temperature is -10~-15 DEG C, during which thaws 2 times, and continue to freeze.Third part seed with Second part of seed is the same, is first placed under tree and pulp is allowed to rot, and collects seed and dries in the shade, is first placed in refrigerator (temperature in mid-October Degree is -10 DEG C), December carries out cold stratification again.It is sampled after each progress of three kinds of processing 90 days, measurement Europe Lee seed Viability and germination rate.Test result is as follows:
The comparison of Europe Lee's seed vigor (%) under 1 three kinds of processing methods of table
As can be seen from Table 1, the life rate of sampling seed is 62.5% after the outer low-temperature stratification of Europe Lee seed chamber, substantially less than The seed vigor of sand storage and refrigerator freezing intermittent warming on the spot, the refrigerator freezing seed between Sand culture on the spot that relents are raw Vigor difference is not significant.During low-temperature stratification, with the extension of processing time, there is mildew and rot quantity and increases in Europe Lee's seed It is more, therefore the seed for losing viability is more.
The comparison of Europe Lee's seed germination rate (%) under 2 three kinds of processing methods of table
From table 2 it can be seen that after refrigerator freezing intermittent warming Europe Lee seed germination rate highest, be 76.5%, it is significantly high The germination rate of Europe Lee seed after low-temperature stratification, but with difference is not shown between the germination rate of Europe Lee's seed after Sand culture on the spot It writes.
Test result shows that Europe Lee's seed can be improved than cold stratification by freezing intermittent warming method using refrigerator (cabinet) Germination percentage.
Specific embodiments of the present invention are described above.It is to be appreciated that the invention is not limited to above-mentioned Particular implementation, those skilled in the art can make a variety of changes or modify within the scope of the claims, this not shadow Ring substantive content of the invention.In the absence of conflict, the feature in embodiments herein and embodiment can any phase Mutually combination.

Claims (2)

1. a kind of method for improving Europe Lee percentage of seedgermination, it is characterised in that: use 2-3 intermittent warming Ou Licheng of low temperature refrigerator Ripe wet seed.
2. a kind of method for improving Europe Lee percentage of seedgermination as described in claim 1, characterized by the following steps:
S1, the collecting fruit after the plum fruit full maturity of Europe are deposited under tree and allow its natural after-ripening, pulp removed after 3 days, by seed It cleans, band hygroscopic water is loaded in Fibre Bag, spare;
S2, Europe Lee's seed of pack is placed in -10~-15 DEG C of refrigerator-freezer, after freezing processing 30 days, is powered off to refrigerator-freezer, after 5 days It is powered, continues to previous temperature freezing processing 30 days;
S3, after repeating step S22-3 times, Europe Lee's seed is frozen in spare in 0~5 DEG C of refrigerator-freezer;
S4, when outdoor daily mean temperature reaches 6-10 DEG C, after taking out the Europe Lee's seed natural thaw handled well, seed is sowed In wet husky earth, 1 month, Europe Lee's seed can germinate unearthed.
CN201910019125.1A 2019-01-09 2019-01-09 Method for improving germination rate of cerasus humilis seeds Active CN109451915B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1207412A1 (en) * 1983-04-04 1986-01-30 Центральный Республиканский Ботанический Сад Ан Усср Method of presowing treatment of seeds with deep organic dormancy
CN102498783A (en) * 2011-10-21 2012-06-20 芜湖东源新农村开发股份有限公司 Wild cryptotaenia japonica seed dormancy breaking method
CN105210625A (en) * 2015-10-09 2016-01-06 陕西省西安植物园 A kind ofly pass through a moon method for Seeds pression nursery
CN105960881A (en) * 2016-05-16 2016-09-28 江苏农牧科技职业学院 Method for improving germination percentage of jatamans valeriana rhizome

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SU1207412A1 (en) * 1983-04-04 1986-01-30 Центральный Республиканский Ботанический Сад Ан Усср Method of presowing treatment of seeds with deep organic dormancy
CN102498783A (en) * 2011-10-21 2012-06-20 芜湖东源新农村开发股份有限公司 Wild cryptotaenia japonica seed dormancy breaking method
CN105210625A (en) * 2015-10-09 2016-01-06 陕西省西安植物园 A kind ofly pass through a moon method for Seeds pression nursery
CN105960881A (en) * 2016-05-16 2016-09-28 江苏农牧科技职业学院 Method for improving germination percentage of jatamans valeriana rhizome

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
李林光 等: "《苹果实用栽培技术》", 31 January 2018, 中国科学技术出版社 *
浙江农业大学园艺系: "《果菜类蔬菜早熟丰产育苗技术》", 31 January 1978, 上海人民出版社 *
王志梅: "欧李种子休眠发生与解除的研究", 《中国优秀硕士学位论文全文数据库》 *

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