CN109431913A - Skin microecological balance composition and application thereof - Google Patents
Skin microecological balance composition and application thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
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- A61K8/345—Alcohols containing more than one hydroxy group
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- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
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- A61K8/00—Cosmetics or similar toiletry preparations
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- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
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- A61Q5/002—Preparations for repairing the hair, e.g. hair cure
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/02—Preparations for cleaning the hair
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q5/00—Preparations for care of the hair
- A61Q5/12—Preparations containing hair conditioners
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Abstract
The invention relates to a skin microecological balance composition and application thereof, comprising a first balance composition, a second balance composition and a common jujube bark extract; the first balancing composition comprises inulin and gluco-oligosaccharide and the second balancing composition comprises acetyl heptapeptide-4 and caprylyl glycol. The skin microecological balance composition adopts an action mechanism of inhibiting and not killing malassezia, staphylococcus epidermidis and propionibacterium acnes existing in scalp, adopts the common jujube bark extract, the first balance composition (ecological nutrient solution) and the second balance composition (Fensebiome Peptide) to achieve microbial flora balance, and aims at scalp pruritus, desquamation and seborrheic dermatitis, thereby effectively improving the scalp problem.
Description
Technical field
The present invention relates to shield product field is washed, more particularly to a kind of skin microecological balance composition and its application.
Background technique
The World Health Organization promulgate ten health standards in, Article 9 be exactly hair it is glossy, without dandruff.On scalp
It is dispersed with many blood vessels, nerve, sebaceous glands, sweat gland and hair follicle, scalp health is related to health, so skin of head and face
Portion's skin equally needs meticulous cleaning and care.By accumulating within more than 30 years and developing, China's hair washing market for water is come into
The new period of competition and high speed development comprehensively, each producer are all constantly releasing new technology, new product, and the majority of consumers are not yet
The disconnected hair-washing hair-care scalp care products for seeking to be suitble to oneself.In the sense that from now on hair washing product will gradually to wash shield
Cosmetics development becomes people and promotes quality of the life, and perfect self-image enhances the powerful mean of charisma.Look forward to 21 generation
It records, the consumption figure of Chinese hair cosmetic will be continuously increased, and market scale can constantly expand.According to statistics, the perfume (or spice) of current China
Wave market is more than Japan already, close to the U.S., but with worthwhile also less than these developed countries per capita.From the angle of care product
It sees, the hair conditioner market scale in the U.S. is about the half in shampoo market, and the hair conditioner market scale of Japan and its shampoo market
It is close.But the actual conditions in Chinese hair products market are reviewed, scale is less than 1/10th of shampoo, this phenomenon one
Aspect is because current economic condition includes that purchasing power also has certain limitations, and another aspect consumer takes care of hair consciousness also relatively not
Foot.But in view of China's economy will continue to steadily increase from now on, demand of the people to hair-dressing and hair care care products will therewith
Be continuously improved, it may be said that China wash shield cosmetics potential market it is very huge, development prospect is very wide.
In recent years, the demand with people to health and environmental protection is growing, and silicate-containing oil shampoo causes biggish strive
View, reason is to be easy to remain on scalp, although research shows that hair more satin can be made suitable using silicate-containing oil shampoo
Cunning is elegant, but silicate-containing oil shampoo is because its is waterproof, and when hair washing is difficult to clean up, and partially remains in scalp, on hair, for a long time
Using that can cause to deposit on scalp, the problems such as band connections is itched, dandruff, hair are greasy, epilation and hair stimulate, and silicon
Oil gathers on hair will cause hair thicker, hair wash it is dry after it is flat, collapse, be not fluffy.Silicone oil is that a kind of chemistry is lazy
Property substance, be difficult to degrade under natural conditions, in production and use process, silicone oil enters environment with discharge of wastewater, causes environment dirty
Dye.Although being only the addition for eliminating silicone oil on the market in the presence of the shampoo for much declaring no silicone oil, not for formula
Other components are adjusted, this is substantially backwards to the shampoo epoch of no silicone oil addition, can not really solve to consume
The pain spot of person cannot achieve microbial flora balance.
Summary of the invention
Based on this, it is necessary to provide a kind of skin microecological balance composition of achievable microbial flora balance.
A kind of skin microecological balance composition, including the first balance composition, the second balance composition and foreign common jujube bark
Extract；The first balance composition includes inulin and glucose oligosaccharide, and the second balance composition includes acetyl group seven
Peptide -4 and pungent glycol.
It in one of the embodiments, include the component of following mass parts: the first balance 0.02~1 part of composition, second
Balance 0.05~1 part of composition and foreign 0.02~1 part of bark extract of jujube tree.
It in one of the embodiments, include the component of following mass parts: the first balance 0.3~0.7 part of composition, second
Balance 0.4~0.8 part of composition and foreign 0.3~0.7 part of bark extract of jujube tree.
It in one of the embodiments, include the component of following mass parts: the first 0.5 part of composition of balance, the second balance
0.6 part of composition and foreign 0.5 part of bark extract of jujube tree.
In one of the embodiments, in the first balance composition, the matter of the inulin and the glucose oligosaccharide
Amount is than being (0.6~0.74): (0.01~0.15).
In one of the embodiments, in the second balance composition, the acetyl group heptapeptide -4 and the pungent glycol
Mass ratio be (0.45~0.55): (4.5~5.5).
The present invention also provides a kind of above-mentioned skin microecological balance compositions to wash the application in shield product in preparation.
The shield product of washing are selected from shampoo, hair conditioner, hair oil or scalp care Essence in one of the embodiments,.
The present invention also provides one kind to wash shield product, including above-mentioned skin microecological balance composition.
It in one of the embodiments, further include following components: glycerol, propylene glycol, butanediol, oligomerization hyaluronic acid and urine
Bursin.
Skin microecological balance composition of the invention has the advantages that
1) above-mentioned skin microecological balance composition is for chlosma, staphylococcus epidermis and propionic acid Cuo existing for scalp
Sore bacillus takes the mechanism of action for inhibiting not to kill, using foreign jujube tree bark extract, the first balance composition (ecoalimental
Liquid), second balance composition (Fensebiome Peptide), reach microbial flora balance, for itching of the scalp, decortication and
Seborrhea is effectively improved scalp problems.
2) present invention uses inulin and glucose oligosaccharide as scalp ecological nutrient liquid, by promoting proliferation of probiotics,
The proliferation of Reverse transcriptase harmful bacteria.Glucose oligosaccharide belongs to biological selectivity polysaccharide, can be utilized by probiotics, without quilt
Harmful bacteria is absorbed, to promote scalp probiotics to increase, by nutrition with Reverse transcriptase, to prevent the life of harmful bacteria
It is long, protection and maintenance scalp microecosystem.
3) present invention employs foreign jujube tree bark extract (DANDRILYS), saponin(e rich in can be from resisting micro- life
Object, immunological regulation and adjusting three dimensions of fat secretion improve scalp problems.
4) the Fensebiome Peptide that the present invention uses can increase scalp Microbial diversity, promote scalp microorganism
Group's balance, adjusts the metabolic pathway of bacterium, reduces inflammatory factor expression, adjusts immune response, increases epidermal barrier key group
Divide the synthesis of long-chain ceramide, epidermis is promoted closely to connect related gene, protects skin barrier, it is susceptible to reduce its scalp
Property.
5) present invention effectively inhibits NO to discharge using microecological balance technology, be effectively reduced NO burst size for 13.67%~
15.78%, 12.55%~14.66% is reduced compared with control sample, can effectively avoid because microbial flora it is unbalance caused by it is scorching
Inflammation factor release causes itching of the scalp, reduces scalp neurological susceptibility.
6) dandruff self-evaluation is divided into 84.1~91.4 to the present invention after tested, and scalp is substantially without dandruff, pruritis
Self-evaluation reached 77.6~88.4, subject effectively reduces pruritis, and test proves effectively inhibit scalp de-
Bits and reduction itch.
Detailed description of the invention
Fig. 1 is the dynamic equilibrium of scalp Tiny ecosystem and unbalance schematic diagram；
Fig. 2 is the fluorescent microscopy images of the reinforcing cell adhesion experiment of acetyl group heptapeptide -4；
Fig. 3 is acetyl group heptapeptide-semi-finals cell adherence test result figure；
Fig. 4 is the evaluation result figure of the lost model of transepidermal water of acetyl group heptapeptide -4；
Fig. 5 is the experimental result picture that the microbiologic population of acetyl group heptapeptide -4 adheres to；
Fig. 6 is the experimental result picture of the close connection component expression of induction of acetyl group heptapeptide -4；
Fig. 7 is the experimental result picture for promoting the synthesis of long-chain ceramide of acetyl group heptapeptide -4；
Fig. 8 is the experimental result picture of the reduction histamine release of foreign jujube tree bark extract；
Fig. 9 is the experimental result picture of the anti-dandruff effect of foreign jujube tree bark extract；
Figure 10 is the experimental result picture for adjusting sebum and generating of foreign jujube tree bark extract；
Figure 11 is the experimental result picture of the NO inhibiting effect of each control sample and example sample；
The experimental result picture that Figure 12 is evaluated and tested in vitro for the scalp of each control sample and example sample.
Specific embodiment
To facilitate the understanding of the present invention, below will to invention is more fully described, and give it is of the invention compared with
Good embodiment.But the invention can be realized in many different forms, however it is not limited to embodiment described herein.Phase
Instead, purpose of providing these embodiments is makes the disclosure of the present invention more thorough and comprehensive.
Unless otherwise defined, all technical and scientific terms used herein and belong to technical field of the invention
The normally understood meaning of technical staff is identical.Term as used herein in the specification of the present invention is intended merely to description tool
The purpose of the embodiment of body, it is not intended that in the limitation present invention.Term as used herein "and/or" includes one or more phases
Any and all combinations of the listed item of pass.
The skin microecological balance composition of one embodiment of the invention, including the first balance composition, the second balance combination
Object and foreign jujube tree bark extract.Wherein, the first balance composition includes inulin and glucose oligosaccharide, and the second balance composition includes
Acetyl group heptapeptide -4 and pungent glycol.
Skin microecological balance composition of the invention is with mildly for theory, using scalp care as core, to be naturally
Target, it is main that microbial flora balance is reached using foreign jujube tree bark extract, ecological nutrient liquid and Fensebiome Peptide,
For itching of the scalp, decortication and seborrhea, scalp problems are effectively improved, and washes in shield product and applies in scalp.
1, scalp and hair
1.1 scalp structures and barrier
The soil that scalp is depended on for existence as hair, the balance of ecological environment are the basic of hair healthy growth.Scalp
It is most thin one of the skin of human body, blood vessel rich in, it is body skin skin that the speed of aging, which is 6 times of facial skin,
12 times.Scalp area is about 650~700cm2, average follicle population is 200~250/cm2, about 52~79 on forehead
A/cm2, about 42~78/cm on cheek2.The sebum amount of scalp surface secretion can reach 288 μ g/cm in 12h2, and forehead
Sebum amount there was only 144 μ g/cm2。
The sweat gland of scalp, smegma are vigorous, but since the obstruction of hair is difficult to clean, it is rapid to easily cause microorganism
Breeding, sebum is irradiated with ultraviolet radiation and the factors such as environmental pollution influence, and is easy to happen peroxidization, is caused scalp slight
Inflammatory reaction, physics, chemical stimulation brought by certain Hair Stying Products etc. also easily cause scalp irritation.The self-control of scalp
Ability also can gradually fail with the increase at age.These above-mentioned factors cause the reduction of scalp protective barrier, telogen
Extend, scalp hair follicles become smaller, hair follicle capillary is reduced, melanin is formed reduces and scalp is damaged thinning, cause scalp oily
The symptoms such as greasy, sensitive, dry, itch, alopecia, white hair and dandruff.
According to investigations the results show that 70% or more healthy men and women has scalp problems, erythema and inflammation in summer and winter
70% is accounted for, dandruff accounts for 30%, and scalp pimple accounts for 20%.In addition, 101 women to 20~59 years old carry out scalp health state tune
Display is looked into, have rubescent scalp, dry, pimple and dandruff problem accounts for 66%.As it can be seen that the scalp of most of healthy populations is simultaneously
It is non-for health status.
1.2 hair structures and component
The main component of hair is keratin (accounting for about 80% or more), it is made of hair shaft and two part of hair follicle.Hair shaft
Hair cuticle, cortex, three layers of medullary substance can be divided into, hair cuticle is the outermost layer of hair shaft, surrounds hair shaft by 3~7 layers of hair cuticle cell, often
The thickness of a hair cuticle cell is about 3 μm~5 μm.The structure of hair cuticle cell can be divided into epicuticle, exocuticle and endepidermis again,
Wherein High-sulphur protein rich in epicuticle and exocuticle, can resist the influence of extraneous physics and chemical factor.Cortex is tight
It is close around medullary substance, the diameter of cortex cell is about 2 μm~3 μm.Cortex cell is by many crude fibres, fine fibre and fibril
Dimension composition, the diameter of fibrinogen is only 2 × 10-3μm, it is made of the keratin of 2~3 strands of alpha-helixes, then by many fibrinogen shapes
It is 8 × 10 at diameter-3μm fine fibre, then constitute crude fibre by many fine fibres.These above-mentioned fibers are embedded in by height
In the cellular matrix of sulfoprotein composition and fibre bundle closely is formed, wherein in keratin composition, low-sulfur albumen accounts for 60%,
High-sulphur protein accounts for 40%.Contain pigment granule in the matrix of cortex cell, the number of pigment granule decides the color of hair.Marrow
Matter is located at the center of hair shaft, is made of 2~3 layers of polygonal keratinocyte, in addition to the keratin structure of medullary epithelium is beta sheet,
The keratin structure of hair cuticle and cortex cell is alpha-helix.
Recent study discovery has one layer of non-polar lipid covering outer in addition to hair, internal lipids is also contained in hair, it
It is predominantly located in the space between cells between hair cuticle cell and between hair cuticle and cortex cell.The internal lipids are known as cell
Complex compound, the lipid profile of abbreviation CMC, CMC account for the 57% of entire hair shaft fiber, are the primary structure lipids of hair, account for hair
The thickness of the 5%~7% of quality, the CMC between hair cuticle and cortex cell is about 28nm.CMC lipid is in conjunction with protein
Form lipoprotein, a continuous reticular structure can be formed around hair fiber, played between hair cuticle cell, hair cuticle and
Adhesive effect between cortex cell.CMC lipid and people's horny layer of epidermis lipid are same, and main component is ceramide, gallbladder
Sterol sulfuric ester, cholesterol, fatty alcohol and free alkyl alcohol.
In life, the pollution of urban environment, the arrangement of ultraviolet radiation, agent for permanent hair waving, hair dye, bleaching agent and hair
(hair washing, blowing and combing) etc. can cause hair damage to some extent.High-sulfur egg in hair hair cuticle and cortex cell
White is the important component for resisting extraneous various physics and chemical factor, once High-sulphur protein is destroyed, and it is serious to can lead to hair
Fracture.Structured lipid (referring mainly to CMC) inside hair is the important adhesive of hair cuticle and cortex cell, and hair is by above-mentioned
The influence of various physical chemical factors also results in CMC content in hair and reduces, as a result hair cuticle can be made to fall off, hair scale piece is stuck up
It rises.
2 scalp problems
2.1 dandruff
Dandruff is a kind of scalp problems chronic, easy to recur, more typical, compares normal scalp, on scalp or in hair
Laminar scurf occur is dandruff.Under normal circumstances, desquamation and cytothesis are in dynamic equilibrium, completely
The keratinocyte aggregation composition diameter of angling maturation is less than the invisible agglomerate of 0.2mm and falls off.Macroscopic angling is thin
Born of the same parents' agglomerate is then considered as dandruff, and pathologic finding shows as keratinocyte incomplete, undue growth, malalignment etc., and the scales of skin that peel off exists
Microscopically observation is to remaining nucleus.Other than sheet furfur, the main clinical manifestation of dandruff is itch, dry scalp
Tight, shouting pain, there are Sub-clinical inflammation changes.
The current puzzlement there are about 50% or more crowd by dandruff problem in the world, young artificial multiple crowd and
Illness is heavier, and usual male's disease incidence is higher than women, and old man and child morbidity are lower.It is directed to dandruff mechanism at present
In terms of research with means of prevention focuses primarily upon chlosma, and dandruff influence factor is numerous, and pathogenesis is complicated, is related to
Scalp Tiny ecosystem, scalp physiology, many factors such as anti-oxidant are immunized in scalp lipid, and only being described the problem by chlosma is not
No more.
(1) scalp adipose membrane and Tiny ecosystem
Sebum film is as made of the emulsification of the sebum of smegma, the sweat of sweat gland secretion and horn cell sheet lipid
One layer of very thin film, scalp adipose membrane are the sebum films of scalp sites, protect people's scalp health under normal condition.Relative to face
Portion's skin, scalp environment hair is vigorous, and sebaceous glands, the more secretory volumes of sweat gland quantity are big, therefore mineral ion, lipid, moisture etc. are
Microorganism provides suitable growing environment and nutrient source abundant, and microbial origin esterase active is higher.Scalp micropopulation
Plantation density is 103~105/mm2, Propionibacterium, staphylococcus and corynebacteria are most rich in healthy population and dandruff patient
3 kinds of rich bacteriums.Chlosma accounts for the 96% of total number of fungi on scalp, wherein restricted chlosma and Malassezia cilobosa are
Dominant genera, dandruff crowd is compared with healthy population, and chlosma and staphylococcus epidermis quantity increase, and acne propionic acid bar
The decline of bacterium number amount, this may be related with its strict anaerobic.
Most of chlosmas are lipid dependences, lack fatty acid synthetase gene, in order to overcome this defect,
Chlosma has a large amount of fatty acid enzyme encoding gene compared with other fungies, and can use host source property lipid is certainly
The secretion of body nutrient source, a variety of lipase of chlosma source property and phosphatidase etc., rouge needed for not only can compensate for its growth and breeding
Fat acid, and it can be made to survive in the environment of lipid rich.
Chlosma by secretion lipase, can by sebum triglycerides and saturated fatty acid be decomposed into proinflammatory
Unsaturated fatty acid, and these penetrations enter the scratchiness that cuticula not only causes scalp, but also can be with N- methyl-
D-Asp (NMDA) receptor, which combines, makes flow of calcium ions, leads to corneocyte hyperplasia and keratinocyte exception and causes head
The skin graft shape scales of skin that peel off generates.
Squalene is the important component of sebum, can be used as light protective barrier for skin of face first by oxygen
Change, squalene peroxide is proven to have irritation on skin of face and causes keratinocyte proliferation and inflammatory reaction, together
When penetrate into cellular layer influence immune system.The peroxidase of chlosma source property generates hydroxyl by generating hydroperoxides
Base free radical and the generation for causing squalene peroxide.In addition, the lipoxygenase of chlosma source property can peroxidating it is free not
Saturated fatty acid, triglycerides, cholesterol etc., product have cytotoxicity, have an effect in the mechanism of dandruff.
(2) scalp barrier function
Healthy population scalp is equally largely colonized chlosma and has no dandruff symptoms, in dandruff patient and Healthy People
The oleic acid of equivalent is applied on group's scalp, furfur symptom will be further aggravated in dandruff patient, but healthy population does not generate furfur.
Illustrate other than chlosma factor, scalp permeable barrier function is also an important factor for influencing dandruff.
The change of dandruff crowd's scalp barrier function, it may be possible to since scalp by longer-term damage (excessively scald by cleaning
Dye, firmly grab around etc.) after, excite the inflammatory reaction of skin depths signal path, so as to cause scalp inflammation, a series of this barrier
Dysfunction, will lead to epidermal cell abnormal differentiation and hyperplasia causes furfur.In addition, since sebum secretion is excessively vigorous and too late
Shi Qingjie, living habit problem (share comb, pillow cover) etc. and the proliferation for causing scalp chlosma have irritating horse traction
Color bacterium metabolite can destroy scalp barrier function.
Tiny ecosystem influences each other with scalp barrier function, and after scalp barrier function damage, one side external irritant is more
It is easily accessible in cuticula, causes scalp that inflammatory reaction and abnormal furfur occurs.Head caused by another aspect barrier is impaired
Skin pH, antibacterial peptide secretory volume etc. variation, may cause scalp microorganism group at and microbial activity change.In vitro in test
It proves, is conducive to chlosma flourish when medium pH=6.2.
In addition, the lipid of dandruff head smegma penetrates into cuticula and is apparently higher than healthy scalp, i.e. dandruff
There are a large amount of sebaceous gland lipids in scalp cuticula, these substances may destroy the institutional framework of lipid between cells of superficial layer, promote
Make scalp desquamation, and is advantageously possible for field planting of the chlosma in cuticula.Change for lipid content, it may be possible to by
It is proliferated in chlosma and increases transepidermal water loss rate (TWEL value), pH changes, and aquation weakens, and then influences lipid phase
Close the activity of synzyme.
(3) epidermal structure function associated protein
A) silk polyprotein and natural moisturizing factor
The significant decrease of natural moisturizing factor NMF level may be related with impaired barrier.NMF main source is located at cuticula
The silk polymeric protein (FLG) of lower layer hydrolyzes, and the NMF generated after hydrolysis reaches cuticula upper layer.And dandruff is characterized in excessively
Proliferation and quick furfur, corneocyte processing maturation period shorten, therefore, before FLG is hydrolyzed and is generated NMF, keratinocyte
May be mature and reach cuticula upper layer and fall off.
In addition, generating the final step of NMF in FLG, arginine protease -1 and gamma glutamylcyclotransferase can divide
Urea and 2-pyrrolidone-5-carboxylic acid are not converted by arginine and gamma-glutamyl dipeptides, and both enzymes are expressed in dandruff skin
Amount reduces, and for FLG precursor, related synzyme and hydrolase content are reduced, it may be possible to due to barrier function damage, or due to
Water content in cuticula, the variation of pH and cause the activity of enzyme to change.
B) angling desmosome and its hydrolase
Angling desmosome primarily serves connection and fixed function between keratinocyte, can also will adjoin cuticula and closely be connected in one
It rises, mainly desmoglein and desmoglein keratin are constituted.Angling desmosome can be discharged by serine protease-kassinin kinin
Furfur is completed in the hydrolysis such as enzyme, protein lyase and cysteine proteinase-cathepsin.Peripheral angle bridge in dandruff scalp
Grain, which is reduced, may cause the reduction of horn cell bonding force, and keratinocyte is easier to isolate and fall off, but due to sebum increase and
The lipid of structure disturbance, the powerless cohesiveness between keratinocyte increase, and dandruff falls off in the form of sheets.
C) keratin and angling coating
Keratin is the main structural proteins of cuticula, is expressed by keratinocyte and generates and can be formed in iuntercellular
Intermediate wire mesh structure maintains cyto-architectural stability and elasticity.When skin barrier is damaged, the expression of Epidermal Keratin is
Disorder, classical marker of the keratin K1 and K10 as epidermal differentiation, the expression quantity in dandruff epidermis significantly reduces,
Level is gone up after dandruff treatment.For dandruff scalp, barrier factor is removed the reason of keratin unconventionality expression
Outside, it is also possible to have certain relationship with chlosma.
Angling coating is the important feature in keratinocyte differentiation latter stage and its maturation, by the regulation of glutamine transaminage.
In dandruff scalp, participates in the generation of angling film and mature key enzyme transglutaminase -3 and 2 kind of glutamine turn amine
- 1 processive enzyme of enzyme and cathepsin D's expression quantity significantly reduce.Cause this phenomenon that may change with water content of stratum corneum etc.
It is related, it is also possible to related with chlosma metabolin.
The protein content variation to play a crucial role in barrier integrity and scalp cuticular tissue, shows scalp barrier
It is impaired, and prompt incorrect differentiation and proliferation.
D) antibacterial skin and anti-oxidant variation
Ribalgilase is generated by keratinocyte, has the reactive protein of significant confrontation pathogenic microorganisms.In addition,
There are also skins for antibacterial protein in cuticula from albumen, prolactin-inducible protein and Cystatin A, endogenic
Antibacterial protein shows higher repellence to the microorganism of skin infection and invasion.
In dandruff scalp, the expression quantity of above-mentioned several albumen is reduced, some other Buchner's bodies such as apolipoprotein
A-1 and histone H 4 have higher expression quantity, illustrate there are microorganism invasion, the state of this both effectiveness protein expression disorder, can
It can be due to caused by the change of micropopulation in dandruff scalp environment.
The decline of dandruff scalp oxidation resistance, including superoxide dismutase and hydrogen peroxide enzyme level reduce, in head
In scurf sebum, superoxide dismutase, catalase and PARK7 (DJ-1) are reduced, and show more than innate immune defence system
System is affected, and antioxidant defense mechanisms are also affected.The raising of malonaldehyde and Vitamin E levels prompts dandruff scalp oxygen
Change stress action degree to increase, may cause skin enhances the external sensibility of pollution and UV irradiation such as that infects.The above substance
Variation may with microorganism change and metabolite invasion it is related.
E) inflammatory reaction
Inflammatory reaction is one of the feature of scalp skin damage, carries out pathology assessment to scalp tissue, has around blood vessel
In the presence of leucocyte, there are slight inflammatory reactions for surface scalp.The value of 1L-1 α increases in dandruff scalp cuticula, when
When barrier is impaired or epidermis is persistently inflamed cascade reaction, pre-existing 1L-1 α quick release, is exempting from cuticula
Epidemic disease response upstream link can induce the synthesis of a variety of chemotactic factor (CF)s, reinforces T cell proliferation and B cell differentiation, has immunological regulation
Effect.Biomarker when 1L-8 plays the role of chemotactic and activates neutrophil leucocyte and a cascade of response of inflammation, in head
The horizontal also the same rising of its in scurf scalp, when dandruff is reduced, inflammation is reduced in living tissue, level decline.
Spherical, chaff chlosma is found to have activity of phospholipase, and the phosphatidase of chlosma source property can be catalyzed flower
The synthesis of the raw inflammatory factors such as tetraenoic acid and prostaglandin, and inflammation is caused by this path.
3, technological core
From the point of view of domestic shampoo development course, the prevalence of no silicone oil shampoo is apodictic.Seventy or eighty years in 20th century
It is had a single function for shampoo, focuses on cleaning function.Shield unification and submissive effect, Zhi Houxi were washed in shampoo emphasis since 1985
Product function of flooding segments more obvious, pure natural, plant type product first meeting clue, and the idea of people rises to correct hair and carries out
The stage of total care.So far from 2000, product starts to pursue deep layer cleaning and deep layer reparation, people no longer meet simply
It cleans, it is required that health cleaning from inside to outside.
Silicate-containing oil shampoo is used for a long time, silicone oil can be poly- in hair upper layer lamination, causes hair unnatural, greasy, thick and heavy,
It collapses, dry up short-tempered, therefore the life style without silicone oil shampoo as a kind of health is come into being.Without silicone oil market fire
Heat, what is really to wash shield product without silicone oil, and general no silicone oil product only merely removes silicone oil in formula,
This is only the reprint for being not added with silicone oil shampoo the 1970s, and the decline of bring conditioning performance, is the retroversion of technology, because
This not really washes shield product without silicon.
It really without silicone oil product is led under the development of the technologies such as mild surfactant, special grease, natural active products
No silicone oil technology is crossed to realize, is naturally target so that mildly for theory, scalp care is core, it is mainly living with mild surface
Property agent, substitute silicone oil conditioner and effectively nursing scalp at being grouped as, core is scalp maintenance.That is, without silicon
Oil wash protect product should performance it is mild, using rear easy to clean, the effect for keeping hair smooth is equal to containing silicone oil product, most heavy
That wants still has the function of nursing scalp.Scalp care is core effect that no silicone oil washes shield product, and to reach nursing head
The effect of skin, scalp care component is essential, is passed through for different scalp problems using special effective care component
Multi-functional prescription exists solving the problems, such as scalp care and pain spot.
The scalp ecological environment of health is by three kinds of balances to realize: grease balance, colony balance and barrier are sound.Head
Oil, head is itched, dandruff is more, hair is dry, alopecia etc. is all the unbalance caused scalp problems of scalp ecological environment.Scalp Dysbiosis
When, scalp flora environment is unbalance, and harmful bacteria is largely bred, and just will appear the phenomenon that head is itched, and the metabolite of harmful bacteria, stimulates
Histamine release of mast cell causes itch, and chlosma metabolism generates phosphatidase and causes arachidonic acid and prostaglandin, aggravates
Inflammation generates, and destroys skin barrier, increases neurological susceptibility.
Scalp is a unique environment, it has dense terminal hair, a large amount of sweat glands and sebaceous glands and higher relatively wet
Degree, these are that the field planting of microorganism creates advantage, and the atomization of corneocyte provides for the field planting of microorganism
A certain amount of amino acid, while sweat gland secretion minerals, there are also sebaceous glands to provide lipid, they mutually cooperate with provide it is rich
Rich environment.
Dandruff is medically known as capitis pityriasis, it is a kind of skin disease as caused by chlosma and a kind of head
The unsound performance of skin.Its mechanism of production is chlosma mass propagation on scalp, causes the excessive increasing of keratoderma
Raw, so that corneocyte be promoted to fall off extremely with the formation of white or gray scales, this scales of skin that peel off to fall off is dandruff.
Chlosma colonizes on scalp, and the triglyceride in sebaceous glands can be made to decompose, release free fatty acid.It releases
The free fatty acid released infiltrates into cuticula, and then damages the barrier function of skin, and barrier injury causes epidermis excessively to increase
Raw and sebum secretion increases.And the latter provides suitable microenvironment for chlosma proliferation.Private medical service refers to spherical shape
Chlosma and restricted chlosma can be found in dandruff and normal scalp.So the function of immune system of host is just
Certain effect may be played in dandruff is formed whether often.
3.1 first balances composition (ecological nutrient liquid)
Micro- raw flora is important defence line and the first barrier for protecting scalp.The scalp of health needs to maintain microbial flora
The dynamic equilibrium of middle beneficial bacterium and harmful bacteria, the microbial flora in scalp have head staphylococcus, Corynebacterium xerosis, kirschner
The beneficial bacteriums such as library micrococcus luteus and Lactobacillus pentosus and candida albicans, staphylococcus aureus, propionibacterium acnes and not plump
The harmful bacterias such as the spicy bacillus of chaff.Probiotics cannot kill pathogen, but can reach competitive suppression by capturing all foodstuffs
The growth of harmful bacteria processed, scalp dynamic equilibrium and it is unbalance as shown in Figure 1.The present invention is using inulin and glucose oligosaccharide as head
Skin ecological nutrient liquid, by promoting proliferation of probiotics, the proliferation of Reverse transcriptase harmful bacteria, structural formula is distinguished as follows.Portugal
Sugared oligosaccharide belongs to biological selectivity polysaccharide, can be utilized by probiotics, without being absorbed by harmful bacteria, to promote scalp
Probiotics increases, by nutrition with Reverse transcriptase, so that the growth of harmful bacteria is prevented, protection and maintenance scalp microecosystem
System.
3.2 second balances composition (Fensebiome peptide)
It is changed into urban industry economy from rural economy, urbanization increases rapidly, and people no longer pass through as ancestors quickly
It is often interacted with the Nature, cause and contacts reduction naturally, scalp bio-diversity is caused to reduce, so that scalp sensitivity increases
Add, leads to dry scalp, furfur, tight, uncomfortable, or even cause seborrhea.
Scalp has dual skin barrier function, is epidermis and microbiologic population.Scalp microbiologic population passes through difference
Mechanism of action play the effect of protection type barrier, exchanging between microbiologic population and scalp can adjust defense reaction,
Mainly have following three aspect: a) Reverse transcriptase forecloses harmfulness bacterium；B) it is immunoreacted；C) scalp barrier is complete
Property, intercellular connection is strengthened by activation TLRS signal path.
The present invention uses acetyl group heptapeptide -4, has by improving adjusting scalp microbiologic population balance and diversity, increase
Beneficial bacteria, help strengthen scalp barrier, prevent drying.Acetyl group heptapeptide -4 promotes the growth of beneficial bacterium, so that scalp be made to reach most
Good health status, can systematic anti-oxidant, anti-inflammatory, prevent scalp moisture be lost, reinforce pathogen defence, microorganism
Group's balance, improves barrier function, and autoimmunity is stablized.Pungent glycol is then used as solubilizing agents and antibacterial inhibitor and acetyl group seven
- 4 coordinated of peptide.
3.2.1 change microorganism group, strengthen scalp barrier function
(1) beneficial microbe group
Microbial diversity is generally characterized by aromatic index, and aromatic exponential number is lower to show microbial diversity
Lower, this is usually unhealthy related with skin.The region 16rRNA gene V3 can be analyzed by special bioinformatics software
DNA sequencing structure.The present invention effectively promotes Shannon diversity index using acetyl group heptapeptide -4, helps to increase scalp bacterium
Diversity, the new back to nature of discount tare weight, becomes more healthy, helps improve microbiologic population's balance, strengthens micro- life of skin
Object protective barrier.Beneficial to the microorganism group of scalp, bacterial metabolism access can be strengthened, to increase the external nutrition supply of scalp.
(2) strengthen cell adherence
The present invention can effectively strengthen intercellular cell adherence using acetyl group heptapeptide -4, can be fallen off by keratinocyte
Situation is characterized, and effectively dandruff is inhibited to fall off.By the assessment of adhesive tape lift-off technology respectively using placebo (Placebo) and
The scalp keratinocyte dropping situations of the solution of peptide containing 1%Fensebiome, and observed with fluorescence microscope, such as scheme
Shown in 2 and Fig. 3.It is shown experimentally that the scalp handled by acetyl group heptapeptide -4, the keratinocyte to fall off reduce 18.6%,
Dandruff can effectively be inhibited, improve seborrhea, reduce the neurological susceptibility of skin.
(3) scalp barrier is protected
Assess whether there is protective effect to scalp barrier, the model that can scatter and disappear by transepidermal water is evaluated and tested, and is lured with table work
It leads to damage after 48h and scatters and disappears analyzer using moisture of skin to measure trans epidermal water loss (TEWL), prove acetyl group by evaluation and test
Heptapeptide -4 can preferably protect the physical barriers of scalp, prevent drying, as shown in Figure 4.
3.2.2 improve scalp microbiologic population, immunity and barrier integrity
(1) adherency of beneficial microbe group is improved
For 24 hours with Fensebiome Peptide processing Human keratinocytes, then Human keratinocytes are exposed to
30min in the staphylococcus epidermis of fluorescent marker and inactivation staphylococcus aureus, observes Human keratinocytes with microscope
On bacterial adhesion situation, as a result as shown in Figure 5.Experiment shows the acetyl group heptapeptide -4 that the present invention uses, and can effectively promote head
Skin microbiologic population changes, and helps beneficial bacterium survival, staphylococcus epidermis increases by 19%, and staphylococcus aureus reduces
9%.
(2) change the key component in scalp barrier
For 24 hours with 50 μ g/ml Fensebiome Peptide processing number of people skin keratinocyte, reverse transcription is then utilized
PCR (RT-PCR) experiment detection connect TJ and has correlation gene (OCLN, CLDN1, JAM3, TJP1, TJP2, TJP3, ACTA1) with close
Expression, as a result as shown in Figure 6.Experiments have shown that the present invention uses acetyl group heptapeptide -4, the expression of close connection component can induce,
Increase the integrality of head epidermal barrier.
(3) improve scalp barrier function
For 24 hours with 50 μ g/ml Fensebiome peptide processing number of people skin keratinocyte, ultra high efficiency liquid phase is utilized
Chromatography-tandem mass spectrometry measurement ceramide content, as a result as shown in Figure 7.It is demonstrated experimentally that the present invention uses Fensebiome
Peptide can strengthen the key component of epidermal barrier, can promote the synthesis of long-chain ceramide, to barrier function structure and not permeable
Permeability is most important, protects and strengthens the permeable barrier for avoiding water loss, avoid microbial metabolism component chemical invader
Bring changes, and strengthens skin barrier function, improves cuticula integrality.
3.3 foreign jujube tree bark extracts (DANDRILYS)
Pyrithione zinc (ZPT, Pyrithione Zinc) has been used for always anti-dandruff and has washed away since the sixties
In type shampoo, nearest ZPT antidandruff mechanism has been elucidated with, i.e. ZPT can inhibit the growth of fungi, however, this is used for a long time
The side effect of one medicament has also appeared in the newspapers, including allergy, skin irritatin and dry skin.In addition, experiment in vitro channel syndrome
Bright, ZPT can destroy epidermis inner equilibrium, and induce apoptosis of fibroblasts and early ageing, these experiments show that ZPT is that have centainly
Toxicity.
It is a kind of novel work obtained from the plant of South America present invention employs foreign jujube tree bark extract (DANDRILYS)
Property object, foreign jujube is one of most valued trees in Rhamnaceae plant and South America, saponin(e rich in, because similar
Structure, these molecules are classified as a large family, this family member has an extensive pharmacological effect, including it is anti-inflammatory, resist it is micro-
Biology, immunological regulation and multiple functions, have clean, releive, sedative activity, can inhibit dandruff and occur.Foreign jujube tree bark extract
Dandruff problem is reduced mainly around three aspects.
3.3.1 antipruritic characteristic
Histamine can upset skin barrier, further result in and scratch, and mechanical trauma can aggravate skin barrier defect.In order to grind
Study carefully effect of the DANDRILYS to itch process, researcher carries out the main cell i.e. mast cell that itch mechanism is related to
Test.It more precisely, is the histamine for testing mast cell release, result is as shown in Figure 8.The results show that 2% concentration
DANDRILYS substantially reduces histamine release of mast cell (27%), and therefore, DANDRILYS, which has, to releive and antihistamine characteristic, can
To reduce scratching and relevant mechanical trauma.Just because of this, skin of head barrier can be repaired and be protected to foreign jujube extract, and
The vicious circle that middle antidandruff generates.
3.3.2 anti-dandruff effect
In vivo studies is carried out to 34 volunteers (age is between 19~56 years old) with dandruff, with evaluation
The anti-dandruff effect of DANDRILYS.Healthy volunteer with dandruff pre-equilibrates the phase before first undergoing one-week experiment, during which
Volunteers are washed hair using neutral (invalid controls) shampoo.This week in the past after still generate dandruff volunteer will continue into
Row experiment.Only the volunteer with moderate to severe dandruff can participate in clinical trial.In fact, dandruff problem according to
The severity level that professional skin scholar formulates is classified: from 0 point to 10 point, 0 point is healthy scalp, and 10 points is with sheets
The serious dandruff or seborrhea of the adhesiveness scales of skin that peel off, as shown in the table.Volunteer is divided into two groups: first groups, 23
Volunteer uses two kinds of shampoos, with or without the shampoo (invalid controls) of active matter 1%DANDRILYS；Second group, 11
Position volunteer uses two kinds of shampoos, the shampoo containing active matter 1%DANDRILYS or 1% zinc pyrithione (ZPT).Aspiration
Person uses weekly two kinds of shampoos three times, cleans half head using certain shampoo at random, as a result as shown in Figure 9.As a result it shows
Show, DANDRILYS has efficient antidandruff activity, works faster compared with ZPT, effect is stronger.
3.3.3 adjusting sebum to generate
Scalp sebum secretion is the important composition mechanism in dandruff phenomenon.In addition, one of the side effect of ZPT is exactly to lure skin into
Rouge excessive secretion causes hair greasy, foreign jujube tree bark extract long-acting inhibition adjustment mechanism in terms of sebum generation.Therefore, I
Further analyze sebum production rate using 810 PC sebumeter of COURAGE&KHAZAKA SM.Initial skin is detected first
Rouge production rate, then the sebum of measurement in every 3 days.In volunteer in aforementioned dandruff test, every group of some volunteer ginseng
This appended experimental (first group of 10 volunteer, second group of 4 volunteer) is added, the results are shown in Figure 10.The results show that from
It is risen for the first time using shampoo, compares invalid controls and ZPT, the shampoo containing 1%DANDRILYS can substantially reduce scalp
Sebum amount.In addition, the half scalp sebum rate by 1%ZPT hair washing water process is significantly increased after stopping processing.This high skin
Perhaps, rouge amount can be explained stop using ZPT after caused by " Cyclic dart effect ", i.e., because obtaining important source of nutrition, horse traction
Color bacterium breeds rapidly again.In contrast, DANDRILYS can mildly adjust sebum, and purify scalp, supplement hydration sebum
Film rebuilds scalp integrality.So DANDRILYS can adjust sebum secretion, opposite ZPT during processing and after processing
Sebum secretion can be strongly increased.
In conclusion skin microecological balance composition of the invention has the advantages that
1) above-mentioned skin microecological balance composition is for chlosma, staphylococcus epidermis and propionic acid Cuo existing for scalp
Sore bacillus takes the mechanism of action for inhibiting not to kill, using foreign jujube tree bark extract, the first balance composition (ecoalimental
Liquid), second balance composition (Fensebiome Peptide), reach microbial flora balance, for itching of the scalp, decortication and
Seborrhea is effectively improved scalp problems.
2) present invention uses inulin and glucose oligosaccharide as scalp ecological nutrient liquid, by promoting proliferation of probiotics,
The proliferation of Reverse transcriptase harmful bacteria.Glucose oligosaccharide belongs to biological selectivity polysaccharide, can be utilized by probiotics, without quilt
Harmful bacteria is absorbed, to promote scalp probiotics to increase, by nutrition with Reverse transcriptase, to prevent the life of harmful bacteria
It is long, protection and maintenance scalp microecosystem.
3) present invention employs foreign jujube tree bark extract (DANDRILYS), saponin(e rich in can be from resisting micro- life
Object, immunological regulation and adjusting three dimensions of fat secretion improve scalp problems.
4) the Fensebiome Peptide that the present invention uses can increase scalp Microbial diversity, promote scalp microorganism
Group's balance, adjusts the metabolic pathway of bacterium, reduces inflammatory factor expression, adjusts immune response, increases epidermal barrier key group
Divide the synthesis of long-chain ceramide, epidermis is promoted closely to connect related gene, protects skin barrier, it is susceptible to reduce its scalp
Property.
5) present invention effectively inhibits NO to discharge using microecological balance technology, be effectively reduced NO burst size for 13.67%~
15.78%, 12.55%~14.66% is reduced compared with control sample, can effectively avoid because microbial flora it is unbalance caused by it is scorching
Inflammation factor release causes itching of the scalp, reduces scalp neurological susceptibility.
6) present invention is divided into 84.1~91.4 through evaluating and testing dandruff self-evaluation, and scalp is substantially without dandruff, pruritis
Self-evaluation reached 77.6~88.4, subject effectively reduces pruritis, and test proves effectively inhibit scalp de-
Bits and reduction itch.
Its core technology layout is as shown in the table.
In a specific example, skin microecological balance composition includes the component of following mass parts: the first balance group
Close 0.02~1 part of object, the second 0.05~1 part of composition of balance and foreign 0.02~1 part of bark extract of jujube tree.
In a specific example, skin microecological balance composition includes the component of following mass parts: the first balance group
Close 0.3~0.7 part of object, the second 0.4~0.8 part of composition of balance and foreign 0.3~0.7 part of bark extract of jujube tree.
In a specific example, skin microecological balance composition includes the component of following mass parts: the first balance group
Close 0.5 part of object, the second 0.6 part of composition of balance and foreign 0.5 part of bark extract of jujube tree.
In a specific example, in the first balance composition, the mass ratio of inulin and glucose oligosaccharide be (0.6~
0.74): (0.01~0.15).For example, the composition of the first balance composition is as shown in the table, but not limited to this, it can be as needed
Increase and decrease auxiliary material.
| Component | Mass percent % |
| Inulin | 60 |
| Glucose oligosaccharide | 15 |
| Water | 25 |
In a specific example, in the second balance composition, the mass ratio of acetyl group heptapeptide -4 and pungent glycol is (0.45
~0.55): (4.5~5.5).For example, the composition of the second balance composition is as shown in the table, but not limited to this, it can be as needed
Increase and decrease auxiliary material.
The present invention also provides a kind of above-mentioned skin microecological balance compositions in the application washed in shield product.
In a specific example, washes shield product and be selected from shampoo, hair conditioner, hair oil or scalp care Essence.
The present invention also provides one kind to wash shield product, including above-mentioned skin microecological balance composition.
In a specific example, wash further include in shield product following mass parts component: 2~4 parts of glycerol, propylene glycol 1~3
Part, 4~6 parts of butanediol, 0.005~0.015 part of oligomerization hyaluronic acid and 0.1~0.2 part of allantoin.It is appreciated that these groups
It is divided into auxiliary material, is not belonging to active constituent, can according to need and choose whether to add.
The following are specific embodiments.
One, scalp maintenance Essence is prepared
It is prepared into scalp maintenance Essence respectively according to the formula and prescription (surplus is deionized water) of following table, with example sample 2
For configuration technique it is as follows:
Glycerol, butanediol, propylene glycol, oligomerization hyaluronic acid, allantoin and xanthan gum (mass percent are weighed according to formula
0.15%) 75~80 DEG C, are heated to, with 500~600r/min, stirs 30min, until all dissolutions.Then life is sequentially added
State nutrient solution, Fensebiome Peptide and foreign jujube tree bark extract, stir 15min with 200~300r/min, until neutralizing
It completes, all dissolves.Temperature is reduced to 38~45 DEG C, is added preservative PHL (mass percent 1%), with 150~200r/
Min stirs 10min, is cooled to room temperature.It is appreciated that preparation method is without being limited thereto, it can be according to product type and user
Formula is selected, and preservative and other non-active ingredients can choose whether to add as needed.In first balance composition, inulin
Mass ratio with glucose oligosaccharide is 0.6:0.15.In second balance composition, the mass ratio of acetyl group heptapeptide -4 and pungent glycol
For 0.5:5.
Two, effect is evaluated and tested
1, LPS induces RAW264.7 cell to discharge NO inhibiting effect
Test philosophy
LPS (lipopolysaccharides): it is more to be located at outermost one layer of the gram-negative bacterial cell wall lipoid compared with thick (8~10nm)
Glucide is made of lipoid A, core polysaccharide and 3 part of O- specific side chain.Lipopolysaccharides is that endotoxin and important group specificity are anti-
Former (O antigen).Lipopolysaccharides consists of three parts.Lipid A (Lipid A) is the glycolipid for constituting activity of endotoxin, with covalently bonded
Heteroglycan chain is tied, there are two parts: first is that core polysaccharide, is constant in related strain；Another O specificity chain (O-
Specific chain) it is alterable height.The lipopolysaccharides of Escherichia coli is that common B cell promotees to divide in Experimental immunization
Split the factor i.e. polyclonal activator (polyclonal activator).This experiment is produced using LPS induction RAW264.7 cell
Raw allergic reaction, discharges NO (nitric oxide).
Griess assay-NO content test method
NO is easily oxidized to NO in vivo or in aqueous solution2, in acid condition, NO and diazonium salt sulfanilamide (SN) generation diazonium are anti-
It answers, and generates diazonium compound, coupling reaction further occurs with naphthylethenyl diamines for the latter, and the product which generates is dense
Degree has linear relationship with NO concentration, there is maximum absorption band at 540nm.
RAW264.7 cell is drawn into DMEM training base with liquid-transfering gun and dispels cell dispersion, is counted using Hemacytometer
Number cell, then using DMEM come diluting cells, being diluted to concentration is 5 × 104cells/ml.Cell solution difference after dilution
Be inoculated into 96well, each hole be 100 μ l, i.e., 5 × 103cells/well.At 37 DEG C, 5%CO2Incubator in cultivate 24
Hour.Sample to be tested trains base dilution with DMEM, and the concentration after dilution is 1% (concentration is tested by MTT, and result is nontoxic).
After cell culture 24 hours, the whether complete adherent growth of cell is observed, if the complete adherent growth of cell, original training base is moved
It removes, is washed with DPBS.After DPBS is removed, it is separately added into the ready sample in front.The solubility of sample is according to toxotest
Result select safe concentration 1% (20 μ l/well).After sample is added, 37 DEG C are put into, cultivates 20 in the incubator of 5%CO2
Hour.After 20 hours, on the culture medium to new 96Well that pipettes 50 μ l, the 1%Griess reagent of same volume is added,
After being sufficiently mixed, reacted 10 minutes in dark place.Utilize the absorption photometric value of ELISA reader test sample at 540nm.Meter
Calculate NO inhibiting effect (NO Inhibition%)=((" OD " _ Control- " OD " _ Sample)/" OD " _ Control) *
100%, in which: " OD " _ Control is the absorption photometric value of blank sample, and " OD " _ Sample is the absorption photometric of sample to be tested
Value.
Test result
Each control sample and the testing result of example sample are as shown in following table and Figure 11.
| Project | NO burst size reduces (%) | Standard deviation |
| Control sample 1 | 1.12 | 0.77 |
| Control sample 2 | 4.38 | 3.57 |
| Control sample 3 | 5.47 | 2.80 |
| Control sample 4 | 7.03 | 5.42 |
| Example sample 1 | 13.67 | 5.11 |
| Example sample 2 | 15.24 | 5.51 |
| Example sample 3 | 15.78 | 3.57 |
Conclusion: the composition of present example sample effectively inhibits NO to discharge using microecological balance technology, and NO is effectively reduced
Burst size is 13.67%~15.78%, and inhibiting rate improves 12.55%~14.66% compared with control sample, can effectively avoid
Because microbial flora it is unbalance caused by inflammatory factor release cause itching of the scalp, reduce scalp neurological susceptibility.
2, scalp is evaluated and tested in vitro
Evaluating object
Dandruff, itch group are included in standard: it is included in 21 subjects altogether, the age 20~50 years old, wherein male 14, female 7,
By different degrees of dandruff, itch status, it is ready to participate in experiment and signs informed consent form, is surveyed in selected experiment and later follow-up
On the examination same day, avoid strenuous exercise.Tester is randomly divided into 1~7 group with 2 male and 1 female for one group.
Test agent
Subject is divided into 7 groups, each control sample and example sample are evaluated and tested respectively.The specified same washing of subject
After shampoo hair washing, scalp is smeared using corresponding test specimens, every two days using primary.Application method is as follows: first with washing
Shampoo hair washing is primary, and scalp Essence massaging scalp 3min is used after rinsing well, is finally rinsed well again.
Review method
7 groups of subjects are directed to dandruff and itch, carry out assessment marking using 14 days, finally give a mark for every group and take 3 to divide for each person.
It is evaluated according to 0,1,2,3 level Four, subject's self-assessment standard is as shown in the table:
| Rank | Description | Evaluation divides range |
| 0 | There is no dandruff and scratchiness | 75-100 |
| 1 | Slight dandruff and scratchiness | 50-75 |
| 2 | Moderate dandruff and scratchiness | 25-50 |
| 3 | Severe dandruff and scratchiness | 0-25 |
Test result
The evaluation result of each control sample and example sample is as shown in following table and Figure 12.
| Project | Dandruff | Itch |
| Control sample 1 | 55.80 | 45.90 |
| Control sample 2 | 69.40 | 61.50 |
| Control sample 3 | 74.30 | 68.10 |
| Control sample 4 | 61.60 | 49.90 |
| Example sample 1 | 84.10 | 77.60 |
| Example sample 2 | 88.70 | 82.20 |
| Example sample 3 | 91.40 | 88.40 |
Conclusion: the composition of present example sample applies microecological balance technology, through evaluation and test dandruff self-evaluation point
It is 84.1~91.4, scalp is substantially without dandruff, and the self-evaluation of pruritis has reached 77.6~88.4, and subject is effective
Reduce pruritis, it was demonstrated that can effectively inhibit scalp desquamation and reduce itch.
Each technical characteristic of embodiment described above can be combined arbitrarily, for simplicity of description, not to above-mentioned reality
It applies all possible combination of each technical characteristic in example to be all described, as long as however, the combination of these technical characteristics is not deposited
In contradiction, all should be considered as described in this specification.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (10)
1. a kind of skin microecological balance composition, which is characterized in that including first balance composition, second balance composition and
Foreign jujube tree bark extract；The first balance composition includes inulin and glucose oligosaccharide, and the second balance composition includes
Acetyl group heptapeptide -4 and pungent glycol.
2. skin microecological balance composition according to claim 1, which is characterized in that the group including following mass parts
Point: the first 0.02~1 part of composition of balance, the second 0.05~1 part of composition of balance and foreign 0.02~1 part of bark extract of jujube tree.
3. skin microecological balance composition according to claim 1, which is characterized in that the group including following mass parts
Point: the first 0.3~0.7 part of composition of balance, the second 0.4~0.8 part of composition of balance and foreign jujube tree bark extract 0.3~0.7
Part.
4. skin microecological balance composition according to claim 1, which is characterized in that the group including following mass parts
Point: the first 0.5 part of composition of balance, the second 0.6 part of composition of balance and foreign 0.5 part of bark extract of jujube tree.
5. skin microecological balance composition according to any one of claims 1 to 4, which is characterized in that described first is flat
It weighs in composition, the mass ratio of the inulin and the glucose oligosaccharide is (0.6~0.74): (0.01~0.15).
6. skin microecological balance composition according to any one of claims 1 to 4, which is characterized in that described second is flat
It weighs in composition, the mass ratio of the acetyl group heptapeptide -4 and the pungent glycol is (0.45~0.55): (4.5~5.5).
7. the described in any item skin microecological balance compositions of claim 1~6 wash the application in shield product in preparation.
8. application according to claim 7, which is characterized in that the shield product of washing are selected from shampoo, hair conditioner, hair oil or head
Skin nurses Essence.
9. one kind washes shield product, which is characterized in that including the described in any item skin microecological balance compositions of claim 1~6.
10. according to claim 9 wash shield product, which is characterized in that further include following components: glycerol, propylene glycol, fourth two
Alcohol, oligomerization hyaluronic acid and allantoin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811609390.7A CN109431913B (en) | 2018-12-27 | 2018-12-27 | Skin microecological balance composition and application thereof |
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| CN111686049A (en) * | 2020-07-14 | 2020-09-22 | 孙开军 | Antibacterial and acarid-removing shampoo and preparation method thereof |
| CN115209871A (en) * | 2020-03-11 | 2022-10-18 | 株式会社Lg生活健康 | Cosmetic composition for enhancing skin beneficial bacteria |
| CN115915937A (en) * | 2020-06-12 | 2023-04-04 | 帝斯曼知识产权资产管理有限公司 | Novel use |
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| CN109431913B (en) | 2021-05-04 |
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Address after: 410000 No. 390, Guyuan Road, Changsha hi tech Development Zone, Changsha City, Hunan Province Patentee after: Shuiyang Cosmetics Manufacturing Co.,Ltd. Address before: No. 668, Qingshan Road, high tech Development Zone, Changsha, Hunan 410000 Patentee before: HUNAN YUJIA COSMETICS MANUFACTURING Co.,Ltd. |