CN109429616A - A kind of processing method for accelerating davidia involucrata seed germination - Google Patents
A kind of processing method for accelerating davidia involucrata seed germination Download PDFInfo
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- CN109429616A CN109429616A CN201811380475.2A CN201811380475A CN109429616A CN 109429616 A CN109429616 A CN 109429616A CN 201811380475 A CN201811380475 A CN 201811380475A CN 109429616 A CN109429616 A CN 109429616A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
Abstract
The invention discloses a kind of processing method for accelerating davidia involucrata seed germination, include the following steps: that (1) chooses seeds；(2) heap kind is handled；(3) freezing processing；(4) seed soaking；(5) constant temperature vernalization is handled.The present invention provides a kind of processing methods for accelerating davidia involucrata seed germination, have broken the dormant period of Structure of Seed of Davidia Involucrata, have shortened the germination duration of seed, enhance the resistance of seed, improve the germination characteristic of seed, great marketing application value.
Description
Technical field
The invention belongs to seedling growth technical fields, and in particular to a kind of processing method for accelerating davidia involucrata seed germination.
Background technique
Dove tree (Davidia involucrata) be deciduous tree, dove tree is world-renowned precious ornamental tree, Chang Zhi in
Chi Pan, small stream take up rest home, hotel, near exhibition center, and have symbol of peace meaning.Material is heavy, is the first-class use of building
Material can make furniture and make material for carving on it.With good ornamental values and the economic values.But dove tree pericarp thickness is big, it is hard
Hard and compactness is high, influences the mass exchange of seed and external environment, causes fine jade paulownia seed dormancy phase longer, germinates than general kind
It is sub difficult.It is considerably less about the research for accelerating davidia involucrata seed germination now.
Summary of the invention
The purpose of the present invention is being directed to existing problem, a kind of processing method for accelerating davidia involucrata seed germination is provided, is beaten
The dormant period for having broken Structure of Seed of Davidia Involucrata shortens the germination duration of seed, enhances the resistance of seed, and the germination for improving seed is special
Property, great marketing application value.
The present invention is achieved by the following technical solutions:
A kind of processing method for accelerating davidia involucrata seed germination, includes the following steps:
(1) it chooses seeds:
It is spare to choose full grains, character stabilization, the mature Structure of Seed of Davidia Involucrata of no disease and pests harm；
(2) heap kind is handled:
The Structure of Seed of Davidia Involucrata chosen in step (1) and special sandy soil are piled up in supersonic wave cleaning machine in such a way that interlayer is laid with,
One layer of sandy soil are specifically first laid with, one layer of seed is then laid on special sandy soil, then are laid with one layer of special sandy soil on seed,
It amounts to and stacks 5 ~ 8 layers of seed, during heap kind, keeping the moisture content in special sandy soil is 70 ~ 80%, and heap kind takes after handling 4 ~ 7d
It is spare out, the ultrasonication of specific frequency is carried out while the processing of heap kind；
(3) freezing processing:
By in treated the seed investment sterile water of heap kind in step (2), then sterile water is placed in quiet in -40 ~ -20 DEG C of environment
After setting 3 ~ 6h, takes out and immediately break the ice cube of the surface of the seed into pieces, it is spare to separate seed；
(4) seed soaking:
It will be impregnated in seed investment seed-soaking liquid after freezing processing in step (3), the time of immersion treatment is 4 ~ 7d；
(5) constant temperature vernalization is handled:
Seed after seed soaking in step (4) is put into constant temperature germinating box, keeping the temperature in germinating box is 30 ~ 34 DEG C,
Keeping the relative humidity in germinating box is 92 ~ 94%, after vernalization handles 2 ~ 5h, that is, can be taken off sowing.
Further, in the step (2) special sandy soil preparation, include the following steps:
1) taking the soil of long-term plantation Chinese milk vetch to put in a drying box drying to moisture content is 8 ~ 14%, then by the mud after drying
Soil is placed in pulverizer and is crushed, and it is spare to smash it through 10 meshes；
2) 60 ~ 70 parts of soil powder, 34 ~ 38 parts of the fine sand, rough sand 38 ~ 42 in the step 1) of corresponding parts by weight after sieving are weighed
Part, 14 ~ 20 parts of loess stone, 7 ~ 11 parts of lanthanum carbonate in investment agitator tanks, stir and evenly mix postposition jointly with the revolving speed of 500 ~ 700rpm
It is 50 ~ 60% in humidity, places 6 ~ 9d in the environment that temperature is 45 ~ 55 DEG C.
Further, it is 60 ~ 70 DEG C that the temperature in drying box is kept when dry in the step 1).
Further, the partial size of fine sand is 0.3 ~ 0.4mm in the step 2, and the partial size of rough sand is 1 ~ 1.5mm, loess stone
Partial size be 0.2 ~ 0.3mm, the partial size of lanthanum carbonate is 0.04 ~ 0.06mm.
Further, the frequency of ultrasonic wave is 70 ~ 80kHz in the step (2).
Further, each ingredient and corresponding parts by weight in seed-soaking liquid in the step (4) are as follows: 0.5 ~ 0.7 part of abscisic acid, red
1 ~ 1.2 part of mycin, 1.3 ~ 1.7 parts of gallic acid, 2 ~ 3 parts of salicylic acid, 19 ~ 23 parts of dehydrated alcohol, 200 ~ 300 parts of sterile water.
Further, keeping the temperature in seed-soaking liquid in the step (4) when seed soaking is 37 ~ 45 DEG C.
Dove tree pericarp thickness is big, and hard and compactness is high, influences the mass exchange of seed and external environment, leads to fine jade paulownia kind
Sub- dormant period is longer, and germination is more difficult than general seed, seriously limits the popularization of dove tree, therefore the present invention is in existing dove tree kind
It is significantly improved on the basis of subprocessing method, is to choose full grains, character stabilization, no disease and pests harm first
Mature Structure of Seed of Davidia Involucrata, optimizes from provenance, guarantees davidia involucrata seed germination rate.And then by seed and special sandy soil super
The processing of heap kind is carried out in the environment of sound wave, wherein the soil of long-term plantation Chinese milk vetch is added in special sandy soil, it is micro- in soil
Biology has certain corrosiveness, rough sand, fine sand, loess stone and the lanthanum carbonate of addition, with different grains to the kind skin of Structure of Seed of Davidia Involucrata
Diameter is mixed, and during heap kind, facilitates the entrance of oxygen, the effect of ultrasonic wave promotes functional component in sandy soil
Movement accelerates rotting for kind of skin, promotes the development of Structure of Seed of Davidia Involucrata embryo, shorten the germination duration of seed, the carbonic acid added in sandy soil
Lanthanum is a kind of rare-earth compound, can be improved the activity of peroxidase in Structure of Seed of Davidia Involucrata, and phenolic substances increases, Ke Yiyou
The invasion and extension for preventing pathogen of effect, in addition can be improved absorption of the seed to nutrient, and the nutrient absorption for improving seed is special
Property, the resistance of dove tree is improved, also added loess stone, under the booster action of ultrasonic wave, quickly absorbs week during heap kind
Harmful substance in air is enclosed, anion is radiated, purifies air, the effective activity for ensureing embryo.Then seed is placed in ultralow temperature
In environment, low temperature environment helps to kill worm's ovum and the pathogen of the surface of the seed, promotes the quality of Structure of Seed of Davidia Involucrata, it is anti-to improve dove tree
The ability of pest and disease damage, and directly strong ice cube is broken into pieces, can efficiently be softened dove tree kind skin, be accelerated Structure of Seed of Davidia Involucrata and external environment
Mass exchange is carried out, Structure of Seed of Davidia Involucrata is put into seed-soaking liquid impregnate at this time, functional component can promote dove tree in seed-soaking liquid
The activity expression of a variety of enzymes in seed body, if the activity of amylase, peroxidase in Structure of Seed of Davidia Involucrata is significantly improved,
And the raising of both enzymatic activitys, Structure of Seed of Davidia Involucrata cellular respiration intensity can greatly be reinforced, can further promote dove tree kind
The development of sub- embryo improves the vigor of seed, improves the germination characteristic of seed.Vernalization processing is finally carried out in germinating box, is provided
Suitable humidity and temperature, accelerate germination.
The present invention has the advantage that compared with prior art
The present invention provides a kind of processing methods for accelerating davidia involucrata seed germination, have broken the dormant period of Structure of Seed of Davidia Involucrata, have shortened
The germination duration of seed, enhances the resistance of seed, improves the germination characteristic of seed, great marketing application value.
Specific embodiment
Embodiment 1
A kind of processing method for accelerating davidia involucrata seed germination, includes the following steps:
(1) it chooses seeds:
It is spare to choose full grains, character stabilization, the mature Structure of Seed of Davidia Involucrata of no disease and pests harm；
(2) heap kind is handled:
The Structure of Seed of Davidia Involucrata chosen in step (1) and special sandy soil are piled up in supersonic wave cleaning machine in such a way that interlayer is laid with,
One layer of sandy soil are specifically first laid with, one layer of seed is then laid on special sandy soil, then are laid with one layer of special sandy soil on seed,
Amount to and stack 5 layers of seed, during heap kind, keeping the moisture content in special sandy soil is 70%, taken out after heap kind processing 4d it is spare,
The ultrasonication of specific frequency is carried out while the processing of heap kind；
(3) freezing processing:
By in treated the seed investment sterile water of heap kind in step (2), then sterile water is placed in -40 DEG C of environment and stands 3h
Afterwards, it takes out and immediately breaks the ice cube of the surface of the seed into pieces, it is spare to separate seed；
(4) seed soaking:
It will be impregnated in seed investment seed-soaking liquid after freezing processing in step (3), the time of immersion treatment is 4d；
(5) constant temperature vernalization is handled:
Seed after seed soaking in step (4) is put into constant temperature germinating box, keeping the temperature in germinating box is 30 DEG C, is kept
Relative humidity in germinating box is 92%, after vernalization handles 2h, that is, can be taken off sowing.
Further, in the step (2) special sandy soil preparation, include the following steps:
1) taking the soil of long-term plantation Chinese milk vetch to put in a drying box drying to moisture content is 8%, then sets the soil after drying
In being crushed in pulverizer, it is spare to smash it through 10 meshes；
2) 60 parts of soil powder, 34 parts of the fine sand, 38 parts of rough sand, loess stone 14 in the step 1) of corresponding parts by weight after sieving are weighed
In part, 7 parts of lanthanum carbonate common investment agitator tanks, being stirred and evenly mixed with the revolving speed of 500rpm and being placed on humidity is 50%, temperature 45
DEG C environment in place 6d.
Further, it is 60 DEG C that the temperature in drying box is kept when dry in the step 1).
Further, the partial size of fine sand is 0.3mm in the step 2, and the partial size of rough sand is 1mm, and the partial size of loess stone is
0.2mm, the partial size of lanthanum carbonate are 0.04mm.
Further, the frequency of ultrasonic wave is 70kHz in the step (2).
Further, each ingredient and corresponding parts by weight in seed-soaking liquid in the step (4) are as follows: 0.5 part of abscisic acid, gibberellin
1 part, 1.3 parts of gallic acid, 2 parts of salicylic acid, 19 parts of dehydrated alcohol, 200 parts of sterile water.
Further, keeping the temperature in seed-soaking liquid in the step (4) when seed soaking is 37 DEG C.
Embodiment 2
A kind of processing method for accelerating davidia involucrata seed germination, includes the following steps:
(1) it chooses seeds:
It is spare to choose full grains, character stabilization, the mature Structure of Seed of Davidia Involucrata of no disease and pests harm；
(2) heap kind is handled:
The Structure of Seed of Davidia Involucrata chosen in step (1) and special sandy soil are piled up in supersonic wave cleaning machine in such a way that interlayer is laid with,
One layer of sandy soil are specifically first laid with, one layer of seed is then laid on special sandy soil, then are laid with one layer of special sandy soil on seed,
It amounts to and stacks 6.5 layers of seed, during heap kind, keeping the moisture content in special sandy soil is 75%, is taken out after heap kind processing 5.5d standby
With the ultrasonication of progress specific frequency while the processing of heap kind；
(3) freezing processing:
By in treated the seed investment sterile water of heap kind in step (2), then sterile water is placed in -30 DEG C of environment and is stood
After 4.5h, takes out and immediately break the ice cube of the surface of the seed into pieces, it is spare to separate seed；
(4) seed soaking:
It will be impregnated in seed investment seed-soaking liquid after freezing processing in step (3), the time of immersion treatment is 5.5d；
(5) constant temperature vernalization is handled:
Seed after seed soaking in step (4) is put into constant temperature germinating box, keeping the temperature in germinating box is 32 DEG C, is kept
Relative humidity in germinating box is 93%, after vernalization handles 3.5h, that is, can be taken off sowing.
Further, in the step (2) special sandy soil preparation, include the following steps:
1) taking the soil of long-term plantation Chinese milk vetch to put in a drying box drying to moisture content is 11%, then by the soil after drying
It is placed in pulverizer and is crushed, it is spare to smash it through 10 meshes；
2) 65 parts of soil powder, 36 parts of the fine sand, 40 parts of rough sand, loess stone 17 in the step 1) of corresponding parts by weight after sieving are weighed
In part, 9 parts of lanthanum carbonate common investment agitator tanks, being stirred and evenly mixed with the revolving speed of 600rpm and being placed on humidity is 55%, temperature 50
DEG C environment in place 7.5d.
Further, it is 65 DEG C that the temperature in drying box is kept when dry in the step 1).
Further, the partial size of fine sand is 0.35mm in the step 2, and the partial size of rough sand is 1.25mm, the grain of loess stone
Diameter is 0.25mm, and the partial size of lanthanum carbonate is 0.05mm.
Further, the frequency of ultrasonic wave is 75kHz in the step (2).
Further, each ingredient and corresponding parts by weight in seed-soaking liquid in the step (4) are as follows: 0.6 part of abscisic acid, gibberellin
1.1 parts, 1.5 parts of gallic acid, 2.5 parts of salicylic acid, 21 parts of dehydrated alcohol, 250 parts of sterile water.
Further, keeping the temperature in seed-soaking liquid in the step (4) when seed soaking is 41 DEG C.
Embodiment 3
A kind of processing method for accelerating davidia involucrata seed germination, includes the following steps:
(1) it chooses seeds:
It is spare to choose full grains, character stabilization, the mature Structure of Seed of Davidia Involucrata of no disease and pests harm；
(2) heap kind is handled:
The Structure of Seed of Davidia Involucrata chosen in step (1) and special sandy soil are piled up in supersonic wave cleaning machine in such a way that interlayer is laid with,
One layer of sandy soil are specifically first laid with, one layer of seed is then laid on special sandy soil, then are laid with one layer of special sandy soil on seed,
Amount to and stack 8 layers of seed, during heap kind, keeping the moisture content in special sandy soil is 80%, taken out after heap kind processing 7d it is spare,
The ultrasonication of specific frequency is carried out while the processing of heap kind；
(3) freezing processing:
By in treated the seed investment sterile water of heap kind in step (2), then sterile water is placed in -20 DEG C of environment and stands 6h
Afterwards, it takes out and immediately breaks the ice cube of the surface of the seed into pieces, it is spare to separate seed；
(4) seed soaking:
It will be impregnated in seed investment seed-soaking liquid after freezing processing in step (3), the time of immersion treatment is 7d；
(5) constant temperature vernalization is handled:
Seed after seed soaking in step (4) is put into constant temperature germinating box, keeping the temperature in germinating box is 34 DEG C, is kept
Relative humidity in germinating box is 94%, after vernalization handles 5h, that is, can be taken off sowing.
Further, in the step (2) special sandy soil preparation, include the following steps:
1) taking the soil of long-term plantation Chinese milk vetch to put in a drying box drying to moisture content is 14%, then by the soil after drying
It is placed in pulverizer and is crushed, it is spare to smash it through 10 meshes；
2) 70 parts of soil powder, 38 parts of the fine sand, 42 parts of rough sand, loess stone 20 in the step 1) of corresponding parts by weight after sieving are weighed
In part, 11 parts of lanthanum carbonate common investment agitator tanks, being stirred and evenly mixed with the revolving speed of 700rpm and being placed on humidity is 60%, and temperature is
9d is placed in 55 DEG C of environment.
Further, it is 70 DEG C that the temperature in drying box is kept when dry in the step 1).
Further, the partial size of fine sand is 0.4mm in the step 2, and the partial size of rough sand is 1.5mm, the partial size of loess stone
For 0.3mm, the partial size of lanthanum carbonate is 0.06mm.
Further, the frequency of ultrasonic wave is 80kHz in the step (2).
Further, each ingredient and corresponding parts by weight in seed-soaking liquid in the step (4) are as follows: 0.7 part of abscisic acid, gibberellin
1.2 parts, 1.7 parts of gallic acid, 3 parts of salicylic acid, 23 parts of dehydrated alcohol, 300 parts of sterile water.
Further, keeping the temperature in seed-soaking liquid in the step (4) when seed soaking is 45 DEG C.
Comparative example 1
This comparative example 1 compared with Example 2, saves the whole operation of step (2) heap kind processing, method and step in addition to this
It is all the same.
Comparative example 2
This comparative example 2 compared with Example 2, saves the whole operation of step (3) freezing processing, method and step in addition to this
It is all the same.
Comparative example 3
This comparative example 3 compared with Example 2, saves the whole operation of step (4) seed soaking, method and step in addition to this
It is all the same.
Control group
The processing method of existing Structure of Seed of Davidia Involucrata routine.
In order to compare effect of the present invention, the mature Structure of Seed of Davidia Involucrata of full grains, character stabilization, no disease and pests harm is chosen, then
Therefrom the Structure of Seed of Davidia Involucrata of picking is randomly divided into 5 as experimental subjects by the almost completely the same Structure of Seed of Davidia Involucrata of picking weight size
Group, then respectively with the method corresponding position of embodiment 2, comparative example 1, comparative example 2, comparative example 3 and control group
Every group of Structure of Seed of Davidia Involucrata is managed, after processing, measures index of correlation.
Specific experiment correlation data is as shown in table 1 below:
Table 1
By upper table 1 it can be seen that the Structure of Seed of Davidia Involucrata proline content that embodiment 2 is handled is higher than comparative example 1 and control under one's name
Group, and proline content reflects the resistance of plant to a certain extent in plant, the strong plant of resistance often accumulates
It is more, the Structure of Seed of Davidia Involucrata resistance that the present invention is handled is apparently higher than control group 1, and seed vitality increases；Seed is in germination process
In, the content of soluble sugar can decline, and the content for the soluble sugar in Structure of Seed of Davidia Involucrata that the present invention is handled is real significantly lower than comparison
Example and control group are applied, Seed Development metabolism is fast, and seed has good germination characteristic；
In order to further compare effect of the present invention, the mature Structure of Seed of Davidia Involucrata of full grains, character stabilization, no disease and pests harm is chosen, so
Therefrom the Structure of Seed of Davidia Involucrata of picking is randomly divided by the almost completely the same Structure of Seed of Davidia Involucrata of picking weight size as experimental subjects afterwards
5 groups, then respectively with the method corresponding position of embodiment 2, comparative example 1, comparative example 2, comparative example 3 and control group
Every group of Structure of Seed of Davidia Involucrata is managed, after processing, is sowed, the germination percentage of seed is then measured, average duration of germinateing.
Specific experiment correlation data is as shown in table 2 below:
Table 2
The present invention provides a kind of processing methods for accelerating davidia involucrata seed germination it can be seen from upper table 2, have broken stopping for Structure of Seed of Davidia Involucrata
The dormancy phase shortens the germination duration of seed, enhances the resistance of seed, improves the germination characteristic of seed, great marketing
Application value.
Claims (7)
1. a kind of processing method for accelerating davidia involucrata seed germination, which comprises the steps of:
(1) it chooses seeds:
It is spare to choose full grains, character stabilization, the mature Structure of Seed of Davidia Involucrata of no disease and pests harm；
(2) heap kind is handled:
The Structure of Seed of Davidia Involucrata chosen in step (1) and special sandy soil are piled up in supersonic wave cleaning machine in such a way that interlayer is laid with,
One layer of sandy soil are specifically first laid with, one layer of seed is then laid on special sandy soil, then are laid with one layer of special sandy soil on seed,
It amounts to and stacks 5 ~ 8 layers of seed, during heap kind, keeping the moisture content in special sandy soil is 70 ~ 80%, and heap kind takes after handling 4 ~ 7d
It is spare out, the ultrasonication of specific frequency is carried out while the processing of heap kind；
(3) freezing processing:
By in treated the seed investment sterile water of heap kind in step (2), then sterile water is placed in quiet in -40 ~ -20 DEG C of environment
After setting 3 ~ 6h, takes out and immediately break the ice cube of the surface of the seed into pieces, it is spare to separate seed；
(4) seed soaking:
It will be impregnated in seed investment seed-soaking liquid after freezing processing in step (3), the time of immersion treatment is 4 ~ 7d；
(5) constant temperature vernalization is handled:
Seed after seed soaking in step (4) is put into constant temperature germinating box, keeping the temperature in germinating box is 30 ~ 34 DEG C,
Keeping the relative humidity in germinating box is 92 ~ 94%, after vernalization handles 2 ~ 5h, that is, can be taken off sowing.
2. a kind of processing method for accelerating davidia involucrata seed germination according to claim 1, which is characterized in that the step (2)
In special sandy soil preparation, include the following steps:
1) taking the soil of long-term plantation Chinese milk vetch to put in a drying box drying to moisture content is 8 ~ 14%, then by the mud after drying
Soil is placed in pulverizer and is crushed, and it is spare to smash it through 10 meshes；
2) 60 ~ 70 parts of soil powder, 34 ~ 38 parts of the fine sand, rough sand 38 ~ 42 in the step 1) of corresponding parts by weight after sieving are weighed
Part, 14 ~ 20 parts of loess stone, 7 ~ 11 parts of lanthanum carbonate in investment agitator tanks, stir and evenly mix postposition jointly with the revolving speed of 500 ~ 700rpm
It is 50 ~ 60% in humidity, places 6 ~ 9d in the environment that temperature is 45 ~ 55 DEG C.
3. a kind of processing method for accelerating davidia involucrata seed germination according to claim 2, which is characterized in that in the step 1)
It is 60 ~ 70 DEG C that the temperature in drying box is kept when dry.
4. a kind of processing method for accelerating davidia involucrata seed germination according to claim 2, which is characterized in that in the step 2
The partial size of fine sand is 0.3 ~ 0.4mm, and the partial size of rough sand is 1 ~ 1.5mm, and the partial size of loess stone is 0.2 ~ 0.3mm, the grain of lanthanum carbonate
Diameter is 0.04 ~ 0.06mm.
5. a kind of processing method for accelerating davidia involucrata seed germination according to claim 1, which is characterized in that the step (2)
The frequency of middle ultrasonic wave is 70 ~ 80kHz.
6. a kind of processing method for accelerating davidia involucrata seed germination according to claim 1, which is characterized in that the step (4)
Each ingredient and corresponding parts by weight in middle seed-soaking liquid are as follows: 0.5 ~ 0.7 part of abscisic acid, 1 ~ 1.2 part of gibberellin, gallic acid 1.3 ~ 1.7
Part, 2 ~ 3 parts of salicylic acid, 19 ~ 23 parts of dehydrated alcohol, 200 ~ 300 parts of sterile water.
7. a kind of processing method for accelerating davidia involucrata seed germination according to claim 1, which is characterized in that the step (4)
It is 37 ~ 45 DEG C that the temperature in seed-soaking liquid is kept when middle seed soaking.
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| CN108990462A (en) * | 2018-07-04 | 2018-12-14 | 彝良县鸿志义观赏苗木种植专业合作社 | A kind of Structure of Seed of Davidia Involucrata method for culturing seedlings of high-survival rate |
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