CN109371047B - 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 - Google Patents
一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 Download PDFInfo
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- CN109371047B CN109371047B CN201811162774.9A CN201811162774A CN109371047B CN 109371047 B CN109371047 B CN 109371047B CN 201811162774 A CN201811162774 A CN 201811162774A CN 109371047 B CN109371047 B CN 109371047B
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
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- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
- C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
- C07K14/4723—Cationic antimicrobial peptides, e.g. defensins
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/79—Transferrins, e.g. lactoferrins, ovotransferrins
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/35—Fusion polypeptide containing a fusion for enhanced stability/folding during expression, e.g. fusions with chaperones or thioredoxin
Abstract
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CN201811162774.9A CN109371047B (zh) | 2018-09-30 | 2018-09-30 | 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 |
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CN201811162774.9A CN109371047B (zh) | 2018-09-30 | 2018-09-30 | 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 |
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CN109371047A CN109371047A (zh) | 2019-02-22 |
CN109371047B true CN109371047B (zh) | 2021-11-09 |
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CN114014939A (zh) * | 2021-11-23 | 2022-02-08 | 华侨大学 | 一种牛乳铁蛋白肽融合蛋白、编码基因和保鲜剂及其在水果保鲜中的应用 |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1227147A2 (en) * | 1995-06-07 | 2002-07-31 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
CN104593491A (zh) * | 2014-12-31 | 2015-05-06 | 华侨大学 | 一种利用整合宿主因子来提高pcr扩增灵敏度的方法 |
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2018
- 2018-09-30 CN CN201811162774.9A patent/CN109371047B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1227147A2 (en) * | 1995-06-07 | 2002-07-31 | Invitrogen Corporation | Recombinational cloning using engineered recombination sites |
CN104593491A (zh) * | 2014-12-31 | 2015-05-06 | 华侨大学 | 一种利用整合宿主因子来提高pcr扩增灵敏度的方法 |
Non-Patent Citations (1)
Title |
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Chimeric HU-IHF proteins that alter DNA-binding ability;Naoki Goshima等;《gene》;19920901;第118卷(第1期);第97-102位 * |
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Effective date of registration: 20220513 Address after: 266000 household 301, unit 2, building 34, No. 58, Songling Road, Laoshan District, Qingdao, Shandong Patentee after: Zhang Wei Patentee after: Jiang Shimin Patentee after: Xing Dezhong Patentee after: Shanghai Jihao Electronic Technology Co.,Ltd. Address before: Fengze District of Quanzhou city of Fujian Province east of Huaqiao University, 362000 Patentee before: HUAQIAO University Effective date of registration: 20220513 Address after: 264100 building 58, No. 500 Binhai East Road, Mouping District, Yantai City, Shandong Province Patentee after: Huaqiao Bioengineering Technology Co.,Ltd. Address before: 266000 household 301, unit 2, building 34, No. 58, Songling Road, Laoshan District, Qingdao, Shandong Patentee before: Zhang Wei Patentee before: Jiang Shimin Patentee before: Xing Dezhong Patentee before: Shanghai Jihao Electronic Technology Co.,Ltd. |