CN104593491A - 一种利用整合宿主因子来提高pcr扩增灵敏度的方法 - Google Patents
一种利用整合宿主因子来提高pcr扩增灵敏度的方法 Download PDFInfo
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- CN104593491A CN104593491A CN201410848236.0A CN201410848236A CN104593491A CN 104593491 A CN104593491 A CN 104593491A CN 201410848236 A CN201410848236 A CN 201410848236A CN 104593491 A CN104593491 A CN 104593491A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
试剂 | 使用量(μl) | 终浓度 |
2×Phusion Master Mix | 10 | 1× |
Primer1 | 0.1 | 0.018μM |
Primer2 | 0.1 | 0.017μM |
模板DNA | 0.2 | 0.78ng |
灭菌蒸馏水 | 9.6 | |
总体积 | 20 |
Claims (2)
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CN201410848236.0A CN104593491B (zh) | 2014-12-31 | 2014-12-31 | 一种利用整合宿主因子来提高pcr扩增灵敏度的方法 |
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CN201410848236.0A CN104593491B (zh) | 2014-12-31 | 2014-12-31 | 一种利用整合宿主因子来提高pcr扩增灵敏度的方法 |
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CN104593491A true CN104593491A (zh) | 2015-05-06 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105200036A (zh) * | 2015-09-21 | 2015-12-30 | 华侨大学 | 一种利用整合宿主因子来提高基于pcr的基因合成技术灵敏度的方法 |
CN105200037A (zh) * | 2015-09-21 | 2015-12-30 | 华侨大学 | 一种利用非特异性结合蛋白来提高基于pcr的基因合成技术灵敏度的方法 |
CN105441424A (zh) * | 2015-12-08 | 2016-03-30 | 华侨大学 | 一种利用整合宿主因子提高滚环扩增效率的方法 |
CN109371047A (zh) * | 2018-09-30 | 2019-02-22 | 华侨大学 | 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
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2014
- 2014-12-31 CN CN201410848236.0A patent/CN104593491B/zh active Active
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5646019A (en) * | 1989-10-24 | 1997-07-08 | Stratagene | Method for producing primed nucleic acid templates |
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105200036A (zh) * | 2015-09-21 | 2015-12-30 | 华侨大学 | 一种利用整合宿主因子来提高基于pcr的基因合成技术灵敏度的方法 |
CN105200037A (zh) * | 2015-09-21 | 2015-12-30 | 华侨大学 | 一种利用非特异性结合蛋白来提高基于pcr的基因合成技术灵敏度的方法 |
CN105441424A (zh) * | 2015-12-08 | 2016-03-30 | 华侨大学 | 一种利用整合宿主因子提高滚环扩增效率的方法 |
CN109371047A (zh) * | 2018-09-30 | 2019-02-22 | 华侨大学 | 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 |
CN109371047B (zh) * | 2018-09-30 | 2021-11-09 | 华侨大学 | 一种利用蛋白IHF-α构建和表达耐热性抗菌肽融合蛋白的方法 |
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CN104593491B (zh) | 2017-01-04 |
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Effective date of registration: 20220516 Address after: 266000 household 301, unit 2, building 34, No. 58, Songling Road, Laoshan District, Qingdao, Shandong Patentee after: Zhang Wei Patentee after: Jiang Shimin Patentee after: Xing Dezhong Patentee after: Shanghai Jihao Electronic Technology Co.,Ltd. Address before: Fengze District of Quanzhou city of Fujian Province east of Huaqiao University, 362000 Patentee before: HUAQIAO University Effective date of registration: 20220516 Address after: 264100 building 58, No. 500 Binhai East Road, Mouping District, Yantai City, Shandong Province Patentee after: Huaqiao Bioengineering Technology Co.,Ltd. Address before: 266000 household 301, unit 2, building 34, No. 58, Songling Road, Laoshan District, Qingdao, Shandong Patentee before: Zhang Wei Patentee before: Jiang Shimin Patentee before: Xing Dezhong Patentee before: Shanghai Jihao Electronic Technology Co.,Ltd. |
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