CN109349045A - A kind of method of arasaponin accumulation in promotion pseudo-ginseng - Google Patents
A kind of method of arasaponin accumulation in promotion pseudo-ginseng Download PDFInfo
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- CN109349045A CN109349045A CN201811376290.4A CN201811376290A CN109349045A CN 109349045 A CN109349045 A CN 109349045A CN 201811376290 A CN201811376290 A CN 201811376290A CN 109349045 A CN109349045 A CN 109349045A
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- fertilizer
- radix notoginseng
- ginseng
- top dressing
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 244000131316 Panax pseudoginseng Species 0.000 title claims abstract description 16
- 238000009825 accumulation Methods 0.000 title claims abstract description 15
- 235000003181 Panax pseudoginseng Nutrition 0.000 title claims abstract description 13
- 241000180649 Panax notoginseng Species 0.000 claims abstract description 55
- 235000003143 Panax notoginseng Nutrition 0.000 claims abstract description 55
- 239000003337 fertilizer Substances 0.000 claims abstract description 31
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 22
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 claims abstract description 14
- 229940072033 potash Drugs 0.000 claims abstract description 14
- BWHMMNNQKKPAPP-UHFFFAOYSA-L potassium carbonate Substances [K+].[K+].[O-]C([O-])=O BWHMMNNQKKPAPP-UHFFFAOYSA-L 0.000 claims abstract description 14
- 235000015320 potassium carbonate Nutrition 0.000 claims abstract description 14
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 11
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 10
- 235000003140 Panax quinquefolius Nutrition 0.000 claims abstract description 10
- 235000008434 ginseng Nutrition 0.000 claims abstract description 10
- 239000000618 nitrogen fertilizer Substances 0.000 claims description 21
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 8
- 239000011591 potassium Substances 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- 238000003306 harvesting Methods 0.000 claims description 3
- 230000001737 promoting effect Effects 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims 1
- 230000001276 controlling effect Effects 0.000 claims 1
- 229930182490 saponin Natural products 0.000 abstract description 16
- 150000007949 saponins Chemical class 0.000 abstract description 16
- 239000001397 quillaja saponaria molina bark Substances 0.000 abstract description 14
- 241000196324 Embryophyta Species 0.000 abstract description 4
- 238000012271 agricultural production Methods 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 230000004720 fertilization Effects 0.000 description 15
- 235000017709 saponins Nutrition 0.000 description 15
- DHKHZGZAXCWQTA-UHFFFAOYSA-N [N].[K] Chemical compound [N].[K] DHKHZGZAXCWQTA-UHFFFAOYSA-N 0.000 description 11
- 230000035508 accumulation Effects 0.000 description 9
- 230000000243 photosynthetic effect Effects 0.000 description 8
- 241000208340 Araliaceae Species 0.000 description 7
- 230000012010 growth Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 239000002028 Biomass Substances 0.000 description 6
- 230000015572 biosynthetic process Effects 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- 239000005720 sucrose Substances 0.000 description 4
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 238000003786 synthesis reaction Methods 0.000 description 3
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical group [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- KJTLQQUUPVSXIM-UHFFFAOYSA-N DL-mevalonic acid Natural products OCCC(O)(C)CC(O)=O KJTLQQUUPVSXIM-UHFFFAOYSA-N 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- KJTLQQUUPVSXIM-ZCFIWIBFSA-M (R)-mevalonate Chemical compound OCC[C@](O)(C)CC([O-])=O KJTLQQUUPVSXIM-ZCFIWIBFSA-M 0.000 description 1
- 101100152417 Bacillus spizizenii (strain ATCC 23059 / NRRL B-14472 / W23) tarI gene Proteins 0.000 description 1
- -1 Glycosides Mevalonate Chemical class 0.000 description 1
- 101100509110 Leifsonia xyli subsp. xyli (strain CTCB07) ispDF gene Proteins 0.000 description 1
- 229930189092 Notoginsenoside Natural products 0.000 description 1
- 241000208343 Panax Species 0.000 description 1
- 101100126492 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) ispG1 gene Proteins 0.000 description 1
- 239000008186 active pharmaceutical agent Substances 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 229930182470 glycoside Natural products 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 101150014059 ispD gene Proteins 0.000 description 1
- 101150022203 ispDF gene Proteins 0.000 description 1
- 101150068863 ispE gene Proteins 0.000 description 1
- 101150018742 ispF gene Proteins 0.000 description 1
- 101150081094 ispG gene Proteins 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 238000013441 quality evaluation Methods 0.000 description 1
- 230000024053 secondary metabolic process Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 239000000344 soap Substances 0.000 description 1
- 125000000185 sucrose group Chemical group 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- 230000008736 traumatic injury Effects 0.000 description 1
- 150000003648 triterpenes Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/25—Root crops, e.g. potatoes, yams, beet or wasabi
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
- A01C21/005—Following a specific plan, e.g. pattern
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Soil Sciences (AREA)
- Botany (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of methods of arasaponin accumulation in promotion pseudo-ginseng, belong to technical field of medicinal plant cultivation;The method of the present invention imposes the nitrogen potash fertilizer of different proportion to 2 years raw sangqi ginsengs and triennial Radix Notoginseng, while the base for carrying out different proportion is applied or imposed;Experiments have shown that the high 10%-15% of the more commercially available pseudo-ginseng of total saponin content in Radix Notoginseng main root under the fertilizer application formula and method of application;The method of the present invention is simple, efficient, easy to implement, the accumulation of arasaponin is promoted while being applied fertilizer, suitable for the popularization and application in agricultural production.
Description
Technical field
The invention discloses a kind of methods of arasaponin accumulation in promotion pseudo-ginseng, belong to medicinal plant cultivation technology
Field.
Background technique
Radix NotoginsengPanax notoginseng(Bruk) F. H. Chen is Araliaceae Panax herbaceos perennial, with
Its dried root is used as medicine, and has hemostasis promoting blood circulation two-way regulating function, is usually used in treating traumatic injury, sore and toxic, cardiovascular and cerebrovascular
And enterogastric diseases etc..Saponin(e is the main medicinal ingredient of Radix Notoginseng and quality evaluation index, is based on carbon skeleton structure
Triterpenes secondary metabolites.According to " carbon/nitrogen balance " and " growth/differentiation balance " hypothesis, ring of the plant growth in nutrient abundance
More carbohydrate can be distributed when border for its own growth, but growing environment nutrient dificiency makees the limitation of plant growth
With its photosynthesis is greater than, plant can distribute more carbohydrate for synthesizing the compound with defense function.
When nitrogen application excess or deficiency, photosynthesis of plant is suppressed, and inorganic nitrogen lacks or a large amount of accumulations make its C/N
Than unbalance, and cause secondary metabolism disorder.Therefore, amount of application of nitrogen fertilizer nitrogen insufficient or excessive of applying can further suppress notoginsenoside
Synthesis leads to the reduction of Radix Notoginseng quality.So fertilising can influence its saponin content by changing the nutrient situation of Panax notoginseng Growth environment,
Reasonable Combined N-K fertilizer is of great significance for improving Radix Notoginseng quality.
Summary of the invention
The present invention provides a kind of methods of arasaponin accumulation in promotion pseudo-ginseng, and this method is imposed to Radix Notoginseng
The nitrogen potash fertilizer of different proportion, while carrying out base according to different proportion and applying or impose;That is after raw sangqi ginseng transplanting in 1 year to before harvesting, lead to
Amount of application and the method for application of regulation nitrogen potash fertilizer are crossed to promote the accumulation of arasaponin in pseudo-ginseng.
The method of the present invention concrete operations are as follows: 2 years raw sangqi ginsengs (life in 2 years is in the 1st year growth period after raw sangqi ginseng transplanting in 1 year)
Nitrogenous fertilizer and Potassium Fertilizer ratio be mass ratio 1:1.5-1:3, nitrogenous fertilizer (in terms of N) amount of application be 150 kghm-2 - 300
kg·hm-2, 2 years raw sangqi ginseng nitrogenous fertilizer and potash fertilizer are (with K2O meter) it is that base applies 30%, 70% is imposed, dressing time and dosage chase after for May
Fertilizer 20%, top dressing in June 10%, August top dressing 20% and top dressing in October 20%;Triennial Radix Notoginseng (the after the transplanting of triennial i.e. 1 year raw sangqi ginseng
In 2 year growth period) nitrogenous fertilizer and Potassium Fertilizer ratio be mass ratio 1:1.5-1:3, nitrogenous fertilizer (in terms of N) amount of application is 225 kg
hm-2- 450kg·hm-2, triennial Radix Notoginseng nitrogenous fertilizer and potash fertilizer are (with K2O meter) all impose, dressing time and dosage are top dressing in March
20%, top dressing in May 30%, top dressing in June 10%, August top dressing 20% and top dressing in October 20%.
The method of the present invention is reached by the amount of application and ratio of regulation nitrogen potash fertilizer and the means of method of application improves Radix Notoginseng medicine
The purpose of total saponin content in material, while guaranteeing the biomass for not reducing root.Experiments have shown that: pseudo-ginseng under the fertilization mode
The high 10%-15% of the middle more commercially available pseudo-ginseng of content of the total saponins in radix notoginseng;The method of the present invention is simple, efficient, easy to implement, is being applied
The accumulation that arasaponin is promoted while fertile, suitable for the popularization and application in agricultural production.
Detailed description of the invention
Shadow of Fig. 1 Combined N-K fertilizer to content of the total saponins in radix notoginseng in triennial Radix Notoginseng clip (a), main root (b) and rib (c)
It rings;
Influence of Fig. 2 Combined N-K fertilizer to arasaponin Metabolism-Related Genes Expression in triennial Roots of Panax Notoginseng;Wherein a is
Mevalonate route of synthesis related gene, b are MEP approach related gene, and c is other genes;
Influence of Fig. 3 Combined N-K fertilizer to triennial Radix Notoginseng main root biomass;
Influence of Fig. 4 Combined N-K fertilizer to triennial Radix Notoginseng main root glucide;Wherein a is glucose, and b is sucrose, and c is solubility
Total reducing sugar;
Fig. 5 Combined N-K fertilizer is on the photosynthetic influence of triennial Radix Notoginseng;Wherein a is net photosynthesis, and b is transpiration rate, and c is born of the same parents
Between CO2Concentration, d are stomatal conductance.
Specific embodiment
Below by drawings and examples, invention is further described in detail, but the scope of the present invention is not limited to
The content.Unless otherwise specified, it is unless otherwise specified routine using reagent that routinely operation carries out to method in embodiment
Commercially available reagent or the reagent prepared according to a conventional method, percentage is mass percent in method.
Embodiment 1: the method that arasaponin is accumulated in this promotion pseudo-ginseng is as follows:
1st year growth period after raw sangqi ginseng transplanting in 1 year, nitrogenous fertilizer and Potassium Fertilizer ratio are mass ratio 1:2, and nitrogenous fertilizer (in terms of N) is applied
Dosage is 200 kghm-2, nitrogenous fertilizer and potash fertilizer are (with K2O meter) it is that base applies 30%, 70% is imposed, dressing time and dosage are May
Top dressing 20%, top dressing in June 10%, August top dressing 20% and top dressing in October 20%;
2nd year growth period after the transplanting of 1 year raw sangqi ginseng, 3 pieces of experimental field nitrogenous fertilizer and Potassium Fertilizer mass ratio be respectively 1:1.5,
1:2 and 1:3, nitrogenous fertilizer (in terms of N) amount of application are 225 kghm-2 , potash fertilizer is (with K2O meter) 337.5 kghm of dosage difference-2、
450 kg·hm-2、675 kg·hm-2, nitrogenous fertilizer and potash fertilizer all imposes, and dressing time and dosage are March (20%), May
(30%), June (10%), August (20%) and October (20%);Other fertilisings and field management mode are identical as Routine Management mode.
Embodiment 2: using total saponin content in treated the Radix Notoginseng measurement main root of embodiment 1
Roots of Panax Notoginseng, saponin content in measurement clip, main root and rib are excavated out after Radix Notoginseng sprout tumble;It was found that nitrogen potassium ratio is 1:1.5 item
Total saponin content is 16.84%, 13.33% and 10.99% in Radix Notoginseng clip, main root and rib under part, is distinguished than commercially available medicinal material high
10.21%, 10.07% and 10.12%;Total saponin content is respectively in Radix Notoginseng clip, main root and rib under the conditions of nitrogen potassium ratio is 1:2
17.12%, 13.65% and 11.29%, distinguish than commercially available medicinal material high by 12.04%, 12.72% and 13.13%;It was found that nitrogen potassium ratio is 1:3 item
Saponin content is respectively 17.57%, 13.93% and 11.47% in Radix Notoginseng clip, main root and rib under part, is distinguished than commercially available medicinal material high
14.99%, 15.03% and 14.93%(Fig. 1);Illustrate that the rational application of fertilizers can significantly improve Radix Notoginseng medicine compared with Conventional wisdom plantation fertilising
Total saponin content in material.
Embodiment 3: using the relative expression quantity of saponin formation related gene in treated the Radix Notoginseng measurement main root of embodiment 1
Excavate out Roots of Panax Notoginseng before Radix Notoginseng harvesting, after measurement embodiment 1 is handled in Radix Notoginseng main root saponin formation related gene it is opposite
Expression quantity, while using the Radix Notoginseng of habits fertilization as control;It was found that nitrogen potassium ratio is Radix Notoginseng main root soap under the conditions of 1:1.5,1:2,1:3
Glycosides Mevalonate route of synthesis related geneACAT、HMAS、HMCAR、MVK、PMK、MDD、IDI, MEP approach related geneDXPS、DXPR、ispD、ispE、ispF、ispG、HDRAndGGPS、FPS、SS、SE、DS、CASEqual relative expression quantities are significantly high
In habits fertilization (Fig. 2);Illustrate that the fertilization mode applies fertilizer compared with Conventional wisdom and significantly raises saponin formation dependency basis in Radix Notoginseng main root
Because of expression quantity.
Embodiment 4: main root biomass is measured using treated the Radix Notoginseng of embodiment 1
Roots of Panax Notoginseng is excavated out after Radix Notoginseng sprout tumble, measures main root biology content;It was found that Radix Notoginseng main root is raw under the conditions of nitrogen potassium ratio is 1:1.5
Object amount is 22.78, higher than habits fertilization by 19.58%;Biomass is 23.44 in Radix Notoginseng main root under the conditions of nitrogen potassium ratio is 1:2, than practising
Used fertilising is high by 23.04%;It was found that nitrogen potassium ratio is respectively 24.13 for biomass in Radix Notoginseng main root under the conditions of 1:3, it is higher than habits fertilization
26.67%(Fig. 3);Illustrate that the fertilization mode can ensure that Radix Notoginseng main root biomass does not reduce compared with Conventional wisdom fertilising.
Embodiment 5: glucide content is measured using treated the Radix Notoginseng of embodiment 1
Roots of Panax Notoginseng is excavated out after Radix Notoginseng sprout tumble, measures main root glucide content;It was found that nitrogen potassium ratio is Radix Notoginseng master under the conditions of 1:1.5
Glucose, sucrose and Soluble adhesion molecule are respectively the 1.09 of habits fertilization, 1.08 and 1.06 times in root;Nitrogen potassium ratio is 1:2
Under the conditions of in Radix Notoginseng main root glucose, sucrose and Soluble adhesion molecule be respectively the 1.14 of habits fertilization, 1.15 and 1.11 times;
Nitrogen potassium than under the conditions of 1:3 in Radix Notoginseng main root glucose, sucrose and Soluble adhesion molecule be respectively habits fertilization 1.34,
1.34 and 1.20 times (Fig. 4);Illustrate that the fertilization mode can promote the accumulation of glucide compared with Conventional wisdom fertilising, to be Radix Notoginseng
The accumulation of dry matter and saponin(e provides material base.
Embodiment 6: measurement embodiment 1 treated Radix Notoginseng photosynthetic rate
Measurement net photosynthetic rate (being measured using 6400 type photosynthetic rate instrument (U.S.) of LI) before Radix Notoginseng excavation.It was found that nitrogen potassium
Than for Radix Notoginseng net photosynthetic rate under the conditions of 1:1.5 it is higher than habits fertilization by 9.89%;Nitrogen potassium ratio is Radix Notoginseng blade under the conditions of 1:2
Net Photosynthetic Rate is higher than habits fertilization by 18.73%;Radix Notoginseng net photosynthetic rate is higher than habits fertilization under the conditions of nitrogen potassium ratio is 1:3
42.95%(Fig. 5);Illustrate that the photosynthetic rate of Radix Notoginseng can be improved compared with Conventional wisdom fertilising for the fertilization mode, to be glucide
Accumulation establish material base.
Claims (2)
1. a kind of method for promoting arasaponin accumulation in pseudo-ginseng, it is characterised in that: to harvesting after raw sangqi ginseng transplanting in 1 year
Before, promote the accumulation of arasaponin in pseudo-ginseng by regulating and controlling amount of application and the method for application of nitrogen potash fertilizer.
2. the method according to claim 1 for promoting arasaponin accumulation in pseudo-ginseng, it is characterised in that: life in 2 years
The nitrogenous fertilizer and Potassium Fertilizer ratio of Radix Notoginseng are mass ratio 1:1.5-1:3, and nitrogenous fertilizer is in terms of N, and potash fertilizer is with K2O meter;Amount of application of nitrogen fertilizer is
150 kg·hm-2 - 300 kg·hm-2;2 years raw sangqi ginseng nitrogenous fertilizer and potash fertilizer are that base applies 30%, impose 70%, dressing time and use
Amount is top dressing in May 20%, top dressing in June 10%, August top dressing 20% and top dressing in October 20%;
Triennial Radix Notoginseng nitrogenous fertilizer and Potassium Fertilizer ratio are mass ratio 1:1.5-1:3, and nitrogenous fertilizer is in terms of N, and potash fertilizer is with K2O meter;Nitrogenous fertilizer is applied
Dosage is 225 kghm-2 - 450kg·hm-2;Triennial Radix Notoginseng nitrogenous fertilizer and potash fertilizer all impose, and dressing time and dosage are 3
Moon top dressing 20%, top dressing in May 30%, top dressing in June 10%, August top dressing 20% and top dressing in October 20%.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109295080A (en) * | 2018-09-19 | 2019-02-01 | 昆明理工大学 | Panax japonicus majoris β-amyrin synthase gene Pj β-AS purposes |
CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
CN115812550A (en) * | 2023-01-06 | 2023-03-21 | 昆明理工大学 | Method for improving saponin content in panax notoginseng medicinal material |
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CN106396843A (en) * | 2016-08-25 | 2017-02-15 | 文山苗乡三七科技有限公司 | Method for fertilizing pseudo-ginseng |
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CN104496588A (en) * | 2013-07-25 | 2015-04-08 | 文山苗乡三七科技有限公司 | Fertilizer special for panax notoginseng planting and fertilization method thereof |
CN106396843A (en) * | 2016-08-25 | 2017-02-15 | 文山苗乡三七科技有限公司 | Method for fertilizing pseudo-ginseng |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109295080A (en) * | 2018-09-19 | 2019-02-01 | 昆明理工大学 | Panax japonicus majoris β-amyrin synthase gene Pj β-AS purposes |
CN109295080B (en) * | 2018-09-19 | 2021-08-20 | 昆明理工大学 | Application of rhizoma panacis majoris beta-balsamol synthetase gene Pj beta-AS |
CN113598003A (en) * | 2021-09-15 | 2021-11-05 | 昆明理工大学 | Method for improving synthesis of notoginsenoside and application |
CN115812550A (en) * | 2023-01-06 | 2023-03-21 | 昆明理工大学 | Method for improving saponin content in panax notoginseng medicinal material |
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