CN109313798B - 用于生成模拟数字明场ihc或ish图像的方法和图像处理系统 - Google Patents

用于生成模拟数字明场ihc或ish图像的方法和图像处理系统 Download PDF

Info

Publication number
CN109313798B
CN109313798B CN201780034936.8A CN201780034936A CN109313798B CN 109313798 B CN109313798 B CN 109313798B CN 201780034936 A CN201780034936 A CN 201780034936A CN 109313798 B CN109313798 B CN 109313798B
Authority
CN
China
Prior art keywords
image
fluorescent
images
stain
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201780034936.8A
Other languages
English (en)
Chinese (zh)
Other versions
CN109313798A (zh
Inventor
E·克莱曼
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
Original Assignee
F Hoffmann La Roche AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CN109313798A publication Critical patent/CN109313798A/zh
Application granted granted Critical
Publication of CN109313798B publication Critical patent/CN109313798B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T11/00—2D [Two Dimensional] image generation
    • G06T11/001—Texturing; Colouring; Generation of texture or colour
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T5/00—Image enhancement or restoration
    • G06T5/50—Image enhancement or restoration using two or more images, e.g. averaging or subtraction
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10032—Satellite or aerial image; Remote sensing
    • G06T2207/10036—Multispectral image; Hyperspectral image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10056—Microscopic image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/20—Special algorithmic details
    • G06T2207/20212—Image combination
    • G06T2207/20221—Image fusion; Image merging
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30024—Cell structures in vitro; Tissue sections in vitro
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30204—Marker

Landscapes

  • Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Radiology & Medical Imaging (AREA)
  • Medical Informatics (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201780034936.8A 2016-06-10 2017-06-09 用于生成模拟数字明场ihc或ish图像的方法和图像处理系统 Active CN109313798B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP16174025.3 2016-06-10
EP16174025 2016-06-10
PCT/EP2017/064193 WO2017212055A1 (en) 2016-06-10 2017-06-09 System for bright field image simulation

Publications (2)

Publication Number Publication Date
CN109313798A CN109313798A (zh) 2019-02-05
CN109313798B true CN109313798B (zh) 2022-04-01

Family

ID=56131382

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201780034936.8A Active CN109313798B (zh) 2016-06-10 2017-06-09 用于生成模拟数字明场ihc或ish图像的方法和图像处理系统

Country Status (5)

Country Link
US (1) US10846848B2 (enExample)
EP (1) EP3469548B1 (enExample)
JP (1) JP6755975B2 (enExample)
CN (1) CN109313798B (enExample)
WO (1) WO2017212055A1 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3814741A4 (en) * 2018-06-28 2022-03-02 The Regents Of The University Of California Producing a composite image of a stained tissue sample by combining image data
JP2020020791A (ja) * 2018-07-24 2020-02-06 ソニー株式会社 情報処理装置、情報処理方法、情報処理システム、およびプログラム
WO2020022394A1 (en) * 2018-07-24 2020-01-30 Sony Corporation Information processing apparatus and microscope for separating the fluorescence of a fluorescent reageant from the autofluorescence of a specimen
US12020493B2 (en) 2018-07-27 2024-06-25 Ventana Medical Systems, Inc. Systems for automated in situ hybridization analysis
CN112288783B (zh) * 2018-08-22 2021-06-29 深圳市真迈生物科技有限公司 基于图像构建测序模板的方法、碱基识别方法和装置
JP7116175B2 (ja) * 2018-08-22 2022-08-09 オリンパス株式会社 画像処理装置、撮像システム、画像処理装置の作動方法、及び画像処理装置の作動プログラム
US10753875B1 (en) * 2019-01-31 2020-08-25 Rarecyte, Inc. Spectral unmixing of spectroscopic emission images
US20220148177A1 (en) * 2019-06-02 2022-05-12 Essenlix Corporation Fast staining of biomaterials enhanced by image processing and artificial intelligence
CN111380843A (zh) * 2019-06-28 2020-07-07 南京大学 一种高灵敏的可见-近红外双通道激光荧光显微镜的装置设计及其成像方法
EP3767587B1 (de) * 2019-07-19 2024-10-23 Euroimmun Medizinische Labordiagnostika AG Detektion von präsenzen unterschiedlicher antinukleärer antikörper-fluoreszenzmustertypen und vorrichtung hierfür
CN110689477B (zh) * 2019-09-07 2023-01-10 创新奇智(重庆)科技有限公司 一种通用的瑕疵图像模拟方法
JP7404831B2 (ja) * 2019-12-02 2023-12-26 日本電気株式会社 ソーナー画像伝送装置、システム、ソーナー画像受信装置、ソーナー画像伝送方法、及びプログラム
JP7744340B2 (ja) * 2019-12-05 2025-09-25 テンパス・エーアイ・インコーポレイテッド ハイスループット薬物スクリーニングのためのシステムおよび方法
JP2021124861A (ja) * 2020-02-04 2021-08-30 ソニーグループ株式会社 解析装置、解析方法、解析プログラム及び診断支援システム
EP3889886A1 (en) * 2020-04-01 2021-10-06 Leica Instruments (Singapore) Pte. Ltd. Systems, methods and computer programs for a microscope system and for determining a transformation function
WO2022004500A1 (ja) * 2020-06-30 2022-01-06 ソニーグループ株式会社 情報処理装置、情報処理方法、プログラム、顕微鏡システム及び解析システム
AU2021314130A1 (en) 2020-07-20 2023-02-23 Akoya Biosciences, Inc. Processing and imaging tissue samples
CN111766125A (zh) * 2020-07-29 2020-10-13 广州金域医学检验中心有限公司 利用荧光淬灭时间差进行的染色方法及自动染色装置、设备和介质
CN116075831A (zh) * 2020-08-18 2023-05-05 安捷伦科技有限公司 用于深度学习图像分析和虚拟染色的生物样本的组织染色和连续成像
DE102021204805A1 (de) * 2021-05-11 2022-11-17 Carl Zeiss Microscopy Gmbh Bewertungsverfahren für Simulationsmodelle in der Mikroskopie
GB2607604A (en) * 2021-06-08 2022-12-14 Solentim Ltd Brightfield and fluorescence image comparison
CN117529750A (zh) * 2021-06-17 2024-02-06 文塔纳医疗系统公司 使用多重免疫荧光成像的组织学染色的数字合成
CN113539368B (zh) * 2021-08-30 2023-02-03 长春理工大学 荧光图像信号数据存储与颜色分类方法
CN113933275B (zh) * 2021-10-13 2023-10-24 季华实验室 基于生物成像的定量分析方法、分离方法、装置及设备
EP4177826A1 (en) * 2021-11-05 2023-05-10 Technische Universität Dresden Method and system for simulating and predicting histological staining of a tissue section

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102165489A (zh) * 2008-09-16 2011-08-24 赫斯托克斯公司 生物标志物表达的可再现量化
CN102575990A (zh) * 2009-09-29 2012-07-11 通用电气公司 使用荧光图像产生明场图像的系统和方法
CN102667473A (zh) * 2009-10-12 2012-09-12 文塔纳医疗系统公司 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现
JP2015514212A (ja) * 2012-03-30 2015-05-18 クラリエント ダイアグノスティック サービシーズ， インコーポレイテッド 単一試料での免疫蛍光及び蛍光系核酸分析
CN104813366A (zh) * 2012-12-04 2015-07-29 通用电气公司 使用免疫染色掩膜选择性细化ish分析结果的系统和方法
CN105283904A (zh) * 2013-06-03 2016-01-27 文塔纳医疗系统公司 图像自适应的生理上可信的颜色分离

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6195451B1 (en) 1999-05-13 2001-02-27 Advanced Pathology Ststems, Inc. Transformation of digital images
AU2007269654B2 (en) * 2006-06-30 2013-01-10 University Of Southern California Quantifiable internal reference standards for immunohistochemistry and uses thereof
US8639013B2 (en) * 2011-08-17 2014-01-28 General Electric Company System and methods for generating a brightfield image using fluorescent images

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102165489A (zh) * 2008-09-16 2011-08-24 赫斯托克斯公司 生物标志物表达的可再现量化
CN102575990A (zh) * 2009-09-29 2012-07-11 通用电气公司 使用荧光图像产生明场图像的系统和方法
CN102667473A (zh) * 2009-10-12 2012-09-12 文塔纳医疗系统公司 用于组织的增强的病理学测定和多分析物检测的多模态对比和明场背景再现
JP2013507612A (ja) * 2009-10-12 2013-03-04 ベンタナ メディカル システムズ， インコーポレイテッド 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出
JP2015514212A (ja) * 2012-03-30 2015-05-18 クラリエント ダイアグノスティック サービシーズ， インコーポレイテッド 単一試料での免疫蛍光及び蛍光系核酸分析
CN104813366A (zh) * 2012-12-04 2015-07-29 通用电气公司 使用免疫染色掩膜选择性细化ish分析结果的系统和方法
CN105283904A (zh) * 2013-06-03 2016-01-27 文塔纳医疗系统公司 图像自适应的生理上可信的颜色分离

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Analysis of Cancer Marker in Tissues with Hadamard Transform Fluorescence Spectral Microscopic Imaging;H. Xu 等;《Journal of Fluorescence》;20150530;第25卷(第1期);397-402 *
Immunohistochemistry, histopathology and infrared spectral histopathology of colon cancer tissue sections;K.T. Angela 等;《JOURNAL OF BIOPHOTONICS》;20130131;第6卷(第1期);88-100 *

Also Published As

Publication number Publication date
US20200327657A1 (en) 2020-10-15
EP3469548B1 (en) 2020-05-13
CN109313798A (zh) 2019-02-05
WO2017212055A1 (en) 2017-12-14
JP2019530847A (ja) 2019-10-24
JP6755975B2 (ja) 2020-09-16
EP3469548A1 (en) 2019-04-17
US10846848B2 (en) 2020-11-24

Similar Documents

Publication Publication Date Title
CN109313798B (zh) 用于生成模拟数字明场ihc或ish图像的方法和图像处理系统
Fereidouni et al. Microscopy with ultraviolet surface excitation for rapid slide-free histology
JP2019530847A5 (enExample)
JP5540102B2 (ja) 高められた病理学的決定のための複数モダリティコントラストおよび明視野コンテキスト表現、および組織内の複数検体検出
JP5631999B2 (ja) 蛍光画像を用いて明視野画像を生成するためのシステム及び方法
JP6074427B2 (ja) 蛍光画像を用いて明視野画像を生成するためのシステム及び方法
JP4376058B2 (ja) 定量的ビデオ顕微鏡法とそれに関連するシステム及びコンピューターソフトウェアプログラム製品
JP6654634B2 (ja) 不均一なバイオマーカー分布を定量的に分析するための方法、システム及び装置
CN109923401B (zh) 高光谱成像系统
RU2690224C2 (ru) Обследующее устройство для обработки и анализа изображения
EP3752979A1 (en) Transformation of digital pathology images
US20110091081A1 (en) Method and system for analyzing the expression of biomarkers in cells in situ in their tissue of origin
CN109416730A (zh) 用于检测数字病理图像中的病理异常的方法和系统以及用于注释组织载玻片的方法
JP2022504871A (ja) 改善された核検出およびセグメンテーションを可能にする画像強化
JP7418631B2 (ja) マルチチャネル画像における自己蛍光の寄与を算出するシステムおよび方法
JP7011036B2 (ja) 組織標本の分析を支援するためのスライド画像のカラーデコンボリューションのためのシステムおよび方法
CN116888469A (zh) 使用基于荧光的检测来分析被包埋的组织样品
US20100177942A1 (en) Method and apparatus for analyzing imagery data
Kugler et al. GliaMorph: A modular image analysis toolkit to quantify Müller glial cell morphology
US8824769B2 (en) Process and system for analyzing the expression of biomarkers in a cell
Levenson et al. Spectral imaging in preclinical research and clinical pathology
HK40003631A (zh) 用於明场图像模拟的系统
HK40003631B (zh) 用於生成模拟数字明场ihc或ish图像的方法和图像处理系统
Lin et al. Hsien‑Neng Huang, Chun‑Wei Kuo, Yu‑Ling Hung 3, Chia‑Hung Yang 3, Yu‑Han Hsieh 3
HK1171508A (en) Multi-modality contrast and brightfield context rendering for enhanced pathology determination and multi-analyte detection in tissue

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40003631

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant