CN109287924A - 一种解酒保肝的组合物及其制备方法 - Google Patents
一种解酒保肝的组合物及其制备方法 Download PDFInfo
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- CN109287924A CN109287924A CN201811470024.8A CN201811470024A CN109287924A CN 109287924 A CN109287924 A CN 109287924A CN 201811470024 A CN201811470024 A CN 201811470024A CN 109287924 A CN109287924 A CN 109287924A
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Abstract
本发明公开了一种解酒保肝的组合物及其制备方法,包括葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸,蒸馏水;制备方法包括步骤如下:葡萄籽提取物的提取,配料,混合,包装,制成粉末状固体饮料,饮用时加入水进行溶解后饮用,该组合物能明显延缓酒精致小鼠睡眠的潜伏期,减少酒精致小鼠的睡眠时间,同时能够缓解酒精所引起的小鼠体重下降及肝损伤,且无毒副作用,口感好,解酒保肝效果显著,是一款良好的解酒保肝固体饮料。
Description
技术领域
本发明属于药品技术领域,具体涉及一种解酒保肝的组合物及其制备方法
背景技术
酒是人类生活中的主要饮料之一,在中华五千年文明史中,无论是文学艺术、饮食烹饪还是养生保健,酒在生活中都占据着重要位置,于此同时也形成了劝酒、酗酒等发展畸形的中国酒桌文化,正因此,由酒精引起的一系列疾病正危害着人们的健康。当酒精进入人体之后,而仅仅不到10%的酒精会经呼气、尿、汗等排出。90%以上会被胃肠道吸收入血,自门静脉进入肝脏,并通过乙醇脱氢酶氧化为乙醛、肝微粒体乙醇氧化酶系统氧化为乙醛或在肝脏中过氧化物酶体系参与酒精代谢氧化。在代谢过程中,除了产生大量毒性物质乙醛外,还会伴随着大量的NADH生成以及活性氧的产生,大量的乙醛可直接破坏微管结构,影响微管正常功能,此外还会和机体蛋白质结合,使蛋白失活,影响线粒体功能及细胞器膜结构,大量的NADH生成导致NAD+/NADH比值下降,使得机体脂肪酸氧化、三羧酸循环被抑制,造成体内糖、脂质代谢发生紊乱。正常情况下机体的氧化和抗氧化处于平衡状态,酒精的氧化产生大量的活性氧,导致机体氧化-抗氧化失衡,脂质发生过氧化,DNA及蛋白质发生破坏,直接影响细胞正常生理功能,长期的饮酒还会影响胃、脑、心等多种组织及器官的生理功能,甚至导致细胞凋亡。严重影响身体健康。目前市面上也有很多的解酒药品或保健食品,但其解酒作用单一或促进酒精代谢,或抗氧化作用等,其效果不显著甚至还会出现副作用。因此研究一种影响酒精的吸收、分布、代谢、排泄,从多种途径发挥解酒保肝作用的保健品将会有较大的应用前景。
发明内容
本发明其目的在于制备一种解酒保肝的组合物,该解酒保肝组合物包括葡萄籽提取物,葡萄糖,维生素B2等功效成分组成,通过配料,混合,包装,制成粉末状固体饮料。饮用时加入水进行溶解后饮用,口感好,解酒保肝效果显著,是一款良好的解酒保肝固体饮料。
实现上述目的而采用的技术方案,
一种解酒保肝的组合物,包括:葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸;其组成比例如下:
葡萄籽提取物:50-200重量份
果糖:500-2000重量份
葡萄糖:500-2000重量份
维生素B2:5-20重量份
维生素C:3-12重量份
维生素E:5-20重量份
丁二酸:10-40重量份。
一种解酒保肝的组合物的制备方法,包括步骤:
1.葡萄籽提取物准备:将葡萄籽进行粉碎,过40目筛网,得到葡萄籽粉末;加入10倍重量50%乙醇溶液进行70℃回流提取60min,共三次,合并提取液,过滤,得葡萄籽提取液;将葡萄籽提取液减压旋蒸,浓缩并除去乙醇,得葡萄籽提取液浓缩液;进行减压冷冻干燥,得葡萄籽提取物。
2.将葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸按配方进行精确称重,充分混合,然后进行包装
本发明中所用成分作用如下:
葡萄籽提取物:葡萄籽提取物中所含有的花青素、原花青素、花色苷、原花色苷均具有极强的抗氧化及消除自由基等作用,此外,还能保护和稳定VC和VE,有助于VC和VE的吸收和利用,并与VC和VE发生协同抗氧化、抗脂质过氧化等作用。
葡萄糖和果糖:葡萄糖和果糖吸收进入血液,使得血容量增加,加速肾脏排泄,同时也使得酒精排泄增加,此外,吸收进入血液中的酒精90%会在肝脏中进行代谢,在此过程中需要消耗大量的能量,造成低血糖,而吸收进入的葡萄糖和果糖一方面可以纠正低血糖,另一方面可以氧化分解,为酒精的代谢提供能量。
维生素B2:VB2作为机体辅酶,参与机体氧化还原反应的氢传递,从而影响到机体糖、脂、氨基酸等物质的代谢,而饮酒则会导致VB2的短缺,一方面引起酒精代谢发生异常,另一方面还会导致机体其他物质正常代谢发生紊乱。此外,VB2还会引起黄素酶-谷胱甘肽还原酶活性,从而影响机体的抗氧化作用。
丁二酸:作为PH调节剂和调味剂,和葡萄糖、果糖共同调节固体饮料的酸度和甜度,使之成为口感良好的风味固体饮料。
水:大量水的饮入可以对酒精有稀释作用,减缓酒精进入血液速率,此外,饮用水后,血容量增加,肾脏排泄增加,可同时增加酒精的排泄。
有益效果:在饮用上诉饮料后,可以减缓酒精吸收速度,促进其代谢和排泄,减轻酒精对肝脏等器官损害。本组合物口感好,是一款良好的解酒保肝固体饮料。
具体实施方式
下面对本发明的具体实施方式作进一步详细的说明,以帮助本领域的技术人员对本发明的发明构思、技术方案有更完整、准确和深入的理解。
实施例1:
葡萄籽提取物的提取
将葡萄籽进行粉碎,过40目筛,称取200g,加入10倍重量50%乙醇溶液进行70℃回流提取60min,共三次,合并提取液,过滤,将滤液进行减压旋蒸浓缩至体积为200mL,进行冷冻干燥,得葡萄籽提取物11.5g。
实施例2:
一种解酒保肝的组合物,包括:葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸;精确称取葡萄籽提取物:1000mg,果糖:10000mg,葡萄糖:10000mg,维生素B2:100mg,维生素C:60mg,维生素E:100mg,丁二酸:200mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例3:
一种解酒保肝的组合物,包括:维生素B2,维生素C,维生素E,丁二酸;精确称取维生素B2:100mg,维生素C:60mg,维生素E:100mg,丁二酸:200mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例4:
一种解酒保肝的组合物,包括:葡萄糖,果糖;精确称取果糖:10000mg,葡萄糖:10000mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例5:
一种解酒保肝的组合物,包括:葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸;精确称取果糖:10000mg,葡萄糖:10000mg,维生素B2:100mg,维生素C:60mg,维生素E:100mg,丁二酸:200mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例6:
一种解酒保肝的组合物,包括:葡萄籽提取物,葡萄糖,果糖;精确称取葡萄籽提取物:1000mg,果糖:10000mg,葡萄糖:10000mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例7:
一种解酒保肝的组合物,包括:葡萄籽提取物,维生素B2,维生素C,维生素E,丁二酸;精确称取葡萄籽提取物:1000mg,维生素B2:100mg,维生素C:60mg,维生素E:100mg,丁二酸:200mg,充分混合,然后进行包装;饮用时加入150-300mL水溶解后饮用。
实施例8:
醉酒模型建立:购买ICR雄性小鼠18只,适应性喂养3天后随机分为0.12mL/10g、0.16mL/10g、0.20mL/10g,三个剂量组,每组六只,分别给予0.12mL/10g、0.16mL/10g、0.20mL/10g的56度红星二锅头,记录其醉酒率、睡眠时间、死亡率,其中醉酒率和睡眠时间均以翻正反射是否消失为观测指标。具体数据见下表。
剂量 | 睡眠时间 | 醉酒率 | 死亡率 |
0.12mL/10g | 153±14min | 50% | 0% |
0.16mL/10g | 390±21min | 100% | 0% |
0.20mL/10g | 697±43min | 100% | 50% |
根据以上表格数据,在给予0.16mL/10g的56度红星二锅头为最佳造模剂量,造模小鼠表现为全部醉酒且无死亡。
实施例9:
组合物的治疗:购买ICR雄性小鼠78只,适应性喂养3天后随机分为G1、G2、G3、G4、G5、G6、G7、G8、G9、G10、G11、G12、G13共13组,每组小鼠6只,其中G1组不做任何处理,G3组为海王金樽按0.6g/kg剂量给药,G4组为RU21按0.2g/kg剂量给药,G5组为实施例2按1g/kg剂量给药,G6组为实施例2按2g/kg剂量给药,G7组为实施例2按4g/kg剂量给药,G8组为实施例3按0.043g/kg剂量给药,G9组为实施例4按1.86g/kg剂量给药,G10组为实施例5按1.91g/kg剂量给药,G11组为实施例6按1.96g/kg剂量给药,G12组为实施例7按0.136g/kg剂量给药,G13组为葡萄籽提取物按0.093g/kg剂量给药,每天给药前进行称重,各组均灌胃给药,在给予治疗后30min,根据实施例6中所述,G2-G16组小鼠均给予0.16mL/10g的56度红星二锅头进行灌胃,如此反复7天,每天一次。实验第一天记录小鼠睡眠潜伏期和睡眠时间,实验第7天,小鼠眼眶取血,4度静置1小时,3500rpm离心15min,取上清,每组随机挑取3个样本检测谷丙转氨酶、谷草转氨酶,同时解剖小鼠取出肝脏,剪下100mg左右肝组织,按1:9加入生理盐水,匀浆,离心,取上清,检测其丙二醛含量,采用SPSS 19.0(SPSS Inc.Chicago,IL,USA)软件进行统计分析。结果以mean±SD表示。组间比较采用T检验,*p<0.05为差异有显著性统计学差异,**p<0.01为差异有非常显著性统计学差异,具体数据见下表。
综上所述,本发明中所制备的一种解酒保肝的组合物能显著增加小鼠睡眠潜伏期,G7组与模型组G2组比较均具有极显著性差异(**p<0.01),且优于对照药物组海王金樽、RU21及其他药物组合组;G6组(药物组合为:葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E和丁二酸)优于同等组成剂量的G8组(药物组合为:维生素B2,维生素C,维生素E和丁二酸)、G9组(药物组合为:葡萄糖,果糖)、G10组(药物组合为:葡萄糖,果糖,维生素B2,维生素C,维生素E和丁二酸)、G11组(药物组合为:葡萄籽提取物,葡萄糖,果糖)、G12组(药物组合为:葡萄籽提取物,维生素B2,维生素C,维生素E和丁二酸)、G13组(药物为:葡萄籽提取物);G10组优于同等组成剂量的G8组和G9组;G11组优于同等组成剂量的G9组和G13组;G12组优于同等组成剂量的G8组和G13组。G7组均能显著降低小鼠睡眠时间,与模型组G2组比较具有极显著性差异(**p<0.01),且优于对照药物组海王金樽、RU21及其他药物组合组;G6组优于同等组成剂量的G8组、G9组、G10组、G11组、G12组和G13组;G10组优于同等组成剂量的G8组和G9组;G11组优于同等组成剂量的G9组和G13组;G12组优于同等组成剂量的G8组和G13组。在进行造模7天并治疗7天后,各个组合物剂量组均能显著降低由酒精引起的肝损伤及酒精引起的小鼠体重下降,其中G7组谷丙转氨酶与模型组G2组比较均具有极显著降低(**p<0.01),且优于对照药物组海王金樽及其他药物组合组,与对照药物RU21相当;G6组优于同等组成剂量的G8组、G9组、G10组、G11组、G12组和G13组;G10组优于同等组成剂量的G8组和G9组;G11组优于同等组成剂量的G9组和G13组;G12组优于同等组成剂量的G8组和G13组。G7组谷草转氨酶与模型组G2组比较均具有极显著降低(**p<0.01),且优于对照药物组海王金樽及其他药物组合组,与对照药物RU21相当;G6组优于同等组成剂量的G8组、G9组、G10组、G11组、G12组和G13组;G10组优于同等组成剂量的G8组和G9组;G11组优于同等组成剂量的G9组和G13组;G12组优于同等组成剂量的G8组和G13组。G7组丙二醛与模型组G2组比较均具有极显著降低(**p<0.01),且优于对照药物组海王金樽及其他药物组合组,与对照药物RU21相当;G6组优于同等组成剂量的G8组、G9组、G10组、G11组、G12组和G13组;G10组优于同等组成剂量的G8组和G9组;G11组优于同等组成剂量的G9组和G13组;G12组优于同等组成剂量的G8组和G13组。由此可见,实施例2中葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E和丁二酸的药物组合其治疗作用均明显优于维生素B2,维生素C,维生素E和丁二酸的药物组合;明显优于葡萄糖,果糖的药物组合;明显优于葡萄糖,果糖,维生素B2,维生素C,维生素E和丁二酸的药物组合;明显优于葡萄籽提取物,葡萄糖和果糖的药物组合;明显优于葡萄籽提取物,维生素B2,维生素C,维生素E和丁二酸的药物组合以及葡萄籽提取物的单独治疗效果。因此,其是一款既能够解酒,又具备保肝功效的固体饮料,治疗效果优于对照药物海王金樽及RU21,且无毒副作用。
以上实施例仅为说明本发明的技术思想,不能以此限定本发明的保护范围,凡是按照本发明提出的技术思想,在技术方案基础上所做的任何改动,均落入本发明保护范围之内;本发明未涉及的技术均可通过现有技术加以实现。
Claims (7)
1.一种药物组合物,其特征在于,包括:葡萄籽提取物、葡萄糖、果糖、维生素B2、维生素C、维生素E和丁二酸,饮用时加入蒸馏水溶解后饮用;其组成比例如下:
葡萄籽提取物:50-200重量份
果糖:500-2000重量份
葡萄糖:500-2000重量份
维生素B2:5-20重量份
维生素C:3-12重量份
维生素E:5-20重量份
丁二酸:10-40重量份。
2.一种药物组合物,其特征在于,包括:葡萄糖、果糖、维生素B2、维生素C、维生素E和丁二酸,饮用时加入蒸馏水溶解后饮用;其组成比例如下:
果糖:500-2000重量份
葡萄糖:500-2000重量份
维生素B2:5-20重量份
维生素C:3-12重量份
维生素E:5-20重量份
丁二酸:10-40重量份。
3.一种药物组合物,其特征在于,包括:葡萄籽提取物、葡萄糖、果糖,饮用时加入蒸馏水溶解后饮用;其组成比例如下:
葡萄籽提取物:50-200重量份
果糖:500-2000重量份
葡萄糖:500-2000重量份。
4.一种药物组合物,其特征在于,包括:葡萄籽提取物、维生素B2、维生素C、维生素E和丁二酸,饮用时加入蒸馏水溶解后饮用;其组成比例如下:
葡萄籽提取物:50-200重量份
维生素B2:5-20重量份
维生素C:3-12重量份
维生素E:5-20重量份
丁二酸:10-40重量份。
5.根据权利要求1、3和4所述的一种药物组合物,其特征在于,所述的葡萄籽提取物制备过程如下:
步骤一:将葡萄籽进行粉碎,过40目筛网,得到葡萄籽粉末;
步骤二:将步骤一中的葡萄籽粉末加入10倍重量50%乙醇溶液进行70oC回流提取60min,共三次,合并提取液,过滤,得葡萄籽提取液;
步骤三:将步骤二中的葡萄籽提取液减压旋蒸,浓缩并除去乙醇,得葡萄籽提取液浓缩液
步骤四:将步骤三中的葡萄籽提取液浓缩液进行减压冷冻干燥,得葡萄籽提取物。
6.根据权利要求1、2、3和4所述的一种解酒保肝的组合物的制备方法,包括步骤:
(1)葡萄籽提取物准备:将葡萄籽进行粉碎,过40目筛网,得到葡萄籽粉末;加入10倍重量50%乙醇溶液进行70oC回流提取60min,共三次,合并提取液,过滤,得葡萄籽提取液;将葡萄籽提取液减压旋蒸,浓缩并除去乙醇,得葡萄籽提取液浓缩液;进行减压冷冻干燥,得葡萄籽提取物。
(2)将葡萄籽提取物,葡萄糖,果糖,维生素B2,维生素C,维生素E,丁二酸按配方进行精确称重,充分混合,然后进行包装。
7.如权利要求书1、2、3和4所述的药物组合物在制备治疗解酒或保肝药物中的应用。
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CN106213511A (zh) * | 2016-07-26 | 2016-12-14 | 张韩 | 一种能够解酒保肝的中药制剂及其制备方法 |
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CN108576819A (zh) * | 2018-04-24 | 2018-09-28 | 北京中和鸿业医药科技有限公司 | 一种护肝组合物、保健食品、制备方法及其应用 |
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US20180050015A1 (en) * | 2015-03-23 | 2018-02-22 | Tasly Modern Tcn Garden Co., Ltd. | Pharmaceutical composition containing silybin and ve |
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