CN109220058A - A method of improving cymose buckwheat rhizome percentage of seedgermination - Google Patents
A method of improving cymose buckwheat rhizome percentage of seedgermination Download PDFInfo
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- CN109220058A CN109220058A CN201811284240.3A CN201811284240A CN109220058A CN 109220058 A CN109220058 A CN 109220058A CN 201811284240 A CN201811284240 A CN 201811284240A CN 109220058 A CN109220058 A CN 109220058A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/06—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings
- A01N43/12—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom five-membered rings condensed with a carbocyclic ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/10—Apiaceae or Umbelliferae [Carrot family], e.g. parsley, caraway, dill, lovage, fennel or snakebed
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Abstract
The invention discloses a kind of methods for improving cymose buckwheat rhizome percentage of seedgermination, for the deficiency in the cymose buckwheat rhizome preprocess method of the prior art, using hormone seed soaking, simulating natural environment variation (dry and wet processing), the seed soaking of coriander leachate, the methods of ultrasonication, the germination percentage (reaching 97.8%) of cymose buckwheat rhizome seed is improved, solution cymose buckwheat rhizome percentage of seedgermination is not high, is unfavorable for being widely applied and the technical problems such as excellent production high yield for cymose buckwheat rhizome cultivation.
Description
Technical field
The present invention relates to a kind of methods for improving cymose buckwheat rhizome percentage of seedgermination, belong to field of plant growing technology.
Background technique
Cymose buckwheat rhizome (Fagopyrum dibotrys (D.Don) Hara), also known as RHIZOMA FAGOPYRI CYMOSI, day buckwheat are polygonaceae
(Polygonaceae) Fagopyrum (Fagopyrum Mill.) herbaceos perennial.Southwestern China area is originated in, is distributed in four
The major part provinces and regions such as river, Chongqing, Hunan, Guangxi, Shaanxi, Jiangsu, most wild border in 500~3000m of height above sea level, shrub
Clump, Tian Bian, by road.Because its root tuber, leaf contain a variety of flavonoids and steroidal compounds, can hyoscine, so at present to its exploitation
It is a kind of important there is medicinal valence using the chemistry, pharmacology and clinical research for focusing primarily upon plant and including medicinal ingredient
The resource plant of value.As people are to the gradually understanding of its medical value, supply falls short of demand on the market, and 1999 national
National second level Top-rated protected wild plants are included in, Qianjiang District of Chongqing establishes the wild cymose buckwheat rhizome In-Situ conservation in first, China
Area.
Cymose buckwheat rhizome category perennial plant, the florescence is longer, and seed-setting rate is not high, and modes of reproduction is mainly taken asexual numerous
It grows, this relatively reduces the dependence to zoogamy.Since germination percentage is not satisfactory after cymose buckwheat rhizome artificial seeding, and it is not whole
Together, therefore under normal condition, seed germination rate affects being widely applied for cymose buckwheat rhizome cultivation between 20~30%.
Existing the relevant technologies are as follows:
1, with 0.10%~0.50% boric acid solution or 5.00%~10.00% plant ash leachate to gold before sowing
Buckwheat seed soaking, can obtain good sprouting effect.(Tang Yu, Sun Junxiu, Liu Jianlin, wait cymose buckwheat rhizome comprehensive Utilization Ways and its
Artificial cultivation technique [J] Beijing Agriculture, 2011 (5): 44-46.)
2, under the conditions of 25 DEG C, first with 10% hypochlorite disinfectant 10min, later in 150mgL-1(gibberellin)
GA3It is impregnated in solution for 24 hours, sprouting to cymose buckwheat rhizome seed has certain facilitation, and germination percentage can achieve 76.6%.(king
Macro letter, Yang Sudan, Liu Hong plum difference plant growth regulator is sprouted to cymose buckwheat rhizome seed and influence [J] kind of growth of seedling
Son, 2017 (5): 19-22.)
3. impregnating cymose buckwheat rhizome seed to germination, hair with the PEG (polyethylene glycol) of 5% concentration when germination temperature is 15 DEG C
Bud rate can achieve 80%.(the west effect [J] that Yang Ping, Liang Jian, section magnificence, temperature and PEG concentration sprout cymose buckwheat rhizome seed
Prosperous institute's journal, 2007,21 (4): 17-20).
In the prior art, although cymose buckwheat rhizome percentage of seedgermination is up to 80%, but still there is very big raising space.
Summary of the invention
In consideration of it, the present invention provides a kind of method for improving cymose buckwheat rhizome percentage of seedgermination, cymose buckwheat rhizome percentage of seedgermination is solved
It is not high, it is unfavorable for being widely applied and the technical problems such as excellent production high yield for cymose buckwheat rhizome cultivation.
The technical solution adopted by the present invention is as follows:
A method of improving cymose buckwheat rhizome percentage of seedgermination, comprising the following steps:
1) it pre-processes: taking cymose buckwheat rhizome seed, carry out disinfection after measuring initial moisture content, screen mature, full, no disease and pests harm
Seed；
2) it soaks seed: the seed screened is placed in GA3Impregnated in solution, after taking-up, clean, 25~35 DEG C be dried to it is original
Water content；
3) seed is put into 8~16h in coriander extracting solution, taken out, 25~35 DEG C are dried to initial moisture content, then soak again
After steeping 4~8h, using ultrasonic wave to 5~15min of seed treatment, then impregnates again and be dried to original contain for 25~35 DEG C after 4~8h
Seed is put into coriander extracting solution 25~35 DEG C of taking-up after 8~16h again and is dried to initial moisture content by water；
4) the processed seed placement of step 3) is germinateed in the incubator.
Preferably, GA3The concentration of solution is 100~300mgL-1。
Preferably, seed is in GA3Soaking time in solution is 10~12h.
Preferably, in step 2), the temperature of drying is 30 DEG C.
Preferably, in step 3), the temperature of drying is 28 DEG C.
Preferably, coriander extracting solution the preparation method comprises the following steps:
Coriander to be shredded, the ethyl alcohol of 2~3 times of its weight, 2~3h of refluxing extraction is added, ethyl alcohol is recycled in extracting solution filtering,
Extracting solution is concentrated into 1.5~2.0g/cm3, be cooled to 0~5 DEG C it is spare.
It is furthermore preferred that coriander extracting solution the preparation method comprises the following steps:
Coriander is shredded, the ethyl alcohol of 2 times of its weight, refluxing extraction 2h is added, ethyl alcohol, extracting solution are recycled in extracting solution filtering
It is concentrated into 1.5g/cm3, be cooled to 4 DEG C it is spare.
Preferably, in step 1), the method for disinfection is in the liquor potassic permanganate that seed is immersed in mass concentration and is 3%
30min。
Compared with prior art, the beneficial effects of the present invention are:
In the present invention, for the deficiency in the cymose buckwheat rhizome preprocess method of the prior art, using hormone seed soaking, simulation nature
Environmental change (dry and wet processing), the seed soaking of coriander leachate, the methods of ultrasonication improve the germination percentage of cymose buckwheat rhizome seed,
It can reach 97.8%.
Specific embodiment
Below by specific embodiment, invention is further described in detail.
Experimental method used in the embodiment of the present invention is conventional method unless otherwise specified.
Material used in the embodiment of the present invention, reagent etc., are commercially available unless otherwise specified.
Embodiment 1
A method of improving cymose buckwheat rhizome percentage of seedgermination, comprising the following steps:
1) it pre-processes: taking cymose buckwheat rhizome seed, after measuring initial moisture content, seed is immersed in the Gao Meng that mass concentration is 3%
30min carries out disinfection in sour potassium solution, screens mature, full, no disease and pests harm seed；
2) it soaks seed: the seed screened is placed in 200mgL-1GA310h is impregnated in solution, after taking-up, is cleaned, 30 DEG C of bakings
It does to initial moisture content；
3) seed is put into 12h in coriander extracting solution, taken out, 28 DEG C are dried to initial moisture content, then again after immersion 6h,
Using ultrasonic wave to seed treatment 10min (ultrasonication can change plasma membrane permeability, promote the mass exchange of intraor extracellular,
Enhance physiological metabolism process in seed, promote the development of embryo), it then impregnates again and is dried to initial moisture content for 28 DEG C after 4h, again
Seed is put into coriander extracting solution 28 DEG C of taking-up after 10h and is dried to initial moisture content；
4) the processed seed placement of step 3) is germinateed in the incubator.
Coriander extracting solution the preparation method comprises the following steps:
Coriander Coriandrum sativum L. is shredded, the ethyl alcohol of 2 times of its weight, refluxing extraction 2h, extracting solution is added
Filtering, recycles ethyl alcohol, and extracting solution is concentrated into 1.5g/cm3, be cooled to 4 DEG C it is spare.
Embodiment 2
A method of improving cymose buckwheat rhizome percentage of seedgermination, comprising the following steps:
1) it pre-processes: taking cymose buckwheat rhizome seed, after measuring initial moisture content, seed is immersed in the Gao Meng that mass concentration is 3%
30min carries out disinfection in sour potassium solution, screens mature, full, no disease and pests harm seed；
2) it soaks seed: the seed screened is placed in 100mgL-1GA310h is impregnated in solution, after taking-up, is cleaned, 25 DEG C of bakings
It does to initial moisture content；
3) seed is put into 8h in coriander extracting solution, taken out, 25 DEG C are dried to initial moisture content, then again after immersion 4h,
Using ultrasonic wave to seed treatment 5min, then impregnates again and be dried to initial moisture content for 28 DEG C after 4h, seed is put into Yuan again
28 DEG C, which are taken out, after 10h in Sui extracting solution is dried to initial moisture content；
4) the processed seed placement of step 3) is germinateed in the incubator.
The preparation method of coriander extracting solution is same as Example 1.
Embodiment 3
A method of improving cymose buckwheat rhizome percentage of seedgermination, comprising the following steps:
1) it pre-processes: taking cymose buckwheat rhizome seed, after measuring initial moisture content, seed is immersed in 3% potassium permanganate of mass concentration
30min carries out disinfection in solution, screens mature, full, no disease and pests harm seed；
2) it soaks seed: the seed screened is placed in 300mgL-1GA312h is impregnated in solution, after taking-up, is cleaned, 35 DEG C of bakings
It does to initial moisture content；
3) seed is put into 16h in coriander extracting solution, taken out, 35 DEG C are dried to initial moisture content, then again after immersion 8h,
Using ultrasonic wave to seed treatment 15min, then impregnates again and be dried to initial moisture content for 35 DEG C after 8h, seed is put into Yuan again
35 DEG C, which are taken out, after 16h in Sui extracting solution is dried to initial moisture content；
4) the processed seed placement of step 3) is germinateed in the incubator.
The preparation method of coriander extracting solution is same as Example 1.
Embodiment 4
A method of cymose buckwheat rhizome percentage of seedgermination is improved, the difference with embodiment 3 is,
Coriander extracting solution the preparation method comprises the following steps:
Coriander is shredded, the ethyl alcohol of 2 times of its weight, refluxing extraction 2h is added, ethyl alcohol, extracting solution are recycled in extracting solution filtering
It is concentrated into 1.5g/cm3, be cooled to 0 DEG C it is spare.
Embodiment 5
A method of cymose buckwheat rhizome percentage of seedgermination is improved, the difference with embodiment 3 is,
Coriander extracting solution the preparation method comprises the following steps:
Coriander is shredded, the ethyl alcohol of 3 times of its weight, refluxing extraction 3h is added, ethyl alcohol, extracting solution are recycled in extracting solution filtering
It is concentrated into 2.0g/cm3, be cooled to 5 DEG C it is spare.
Embodiment 6
A method of cymose buckwheat rhizome percentage of seedgermination being improved, the difference with embodiment 3 is, GA3The concentration of solution is
350mg·L-1；Seed is in GA3Soaking time in solution is 8h.
Embodiment 7
A method of cymose buckwheat rhizome percentage of seedgermination being improved, the difference with embodiment 3 is, the method for disinfection is by seed
It is immersed in 10min in 6% liquor potassic permanganate.
Comparative example 1
A method of cymose buckwheat rhizome percentage of seedgermination is improved, the difference with embodiment 3 is,
Step 3): being put into 6h in coriander extracting solution for seed, takes out, 38 DEG C are dried to initial moisture content, then impregnate again
After 3.5h, using ultrasonic wave to seed treatment 3min, then impregnates again and be dried to initial moisture content for 38 DEG C after 3.5h, it again will kind
Son is put into coriander extracting solution 38 DEG C of taking-up after 6h and is dried to initial moisture content.
Comparative example 2
A method of cymose buckwheat rhizome percentage of seedgermination is improved, the difference with embodiment 3 is,
Step 3): being put into 18h in coriander extracting solution for seed, takes out, 20 DEG C are dried to initial moisture content, then impregnate again
After 10h, using ultrasonic wave to seed treatment 20min, then impregnates again and be dried to initial moisture content for 20 DEG C after 10h, it again will kind
Son is put into coriander extracting solution 20 DEG C of taking-up after 18h and is dried to initial moisture content.
Comparative example 3
A method of cymose buckwheat rhizome percentage of seedgermination is improved, the difference with embodiment 3 is,
Step 3): being put into 16h in coriander extracting solution for seed, takes out, 35 DEG C are dried to initial moisture content, again by seed
It is put into coriander extracting solution 35 DEG C of taking-up after 16h and is dried to initial moisture content；
Test example: germination percentage test
Embodiment is individually positioned in culture dish with the processed seed of comparative example and is cultivated.Actual conditions are 30 DEG C, white
It germinates 4 days in the illumination box of its illumination 8h, counts germination percentage, the results are shown in Table 1.
Table 1
| Germination percentage/% | |
| Embodiment 1 | 97.8% |
| Embodiment 2 | 93.4% |
| Embodiment 3 | 93.8% |
| Embodiment 4 | 90.3% |
| Embodiment 5 | 91.0% |
| Embodiment 6 | 83.9% |
| Embodiment 7 | 85.0% |
| Comparative example 1 | 70.7% |
| Comparative example 2 | 75.8% |
| Comparative example 3 | 70.4% |
From test result it is found that the germination percentage that can effectively improve cymose buckwheat rhizome seed using the method for the present invention is (reachable
97.8%).
The above content is specific embodiment is combined, further detailed description of the invention, and it cannot be said that this hair
Bright specific implementation is only limited to these instructions.For those of ordinary skill in the art to which the present invention belongs, it is not taking off
Under the premise of from present inventive concept, a number of simple deductions or replacements can also be made.
Claims (8)
1. a kind of method for improving cymose buckwheat rhizome percentage of seedgermination, which comprises the following steps:
1) it pre-processes: taking cymose buckwheat rhizome seed, carry out disinfection after measuring initial moisture content, screen mature, full, no disease and pests harm kind
Son；
2) it soaks seed: the seed screened is placed in GA3Impregnated in solution, after taking-up, clean, 25~35 DEG C be dried to it is original aqueous
Amount；
3) seed is put into 8~16h in coriander extracting solution, taken out, 25~35 DEG C are dried to initial moisture content, then impregnate 4 again
After~8h, using ultrasonic wave to 5~15min of seed treatment, then impregnate again after 4~8h 25~35 DEG C be dried to it is original aqueous
Seed is put into coriander extracting solution 25~35 DEG C of taking-up after 8~16h again and is dried to initial moisture content by amount；
4) the processed seed placement of step 3) is germinateed in the incubator.
2. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that GA3The concentration of solution is
100~300mgL-1。
3. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that seed is in GA3In solution
Soaking time be 10~12h.
4. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that in step 2), drying
Temperature be 30 DEG C.
5. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that in step 3), drying
Temperature be 28 DEG C.
6. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that the system of coriander extracting solution
Preparation Method are as follows:
Coriander is shredded, the ethyl alcohol of 2~3 times of its weight, 2~3h of refluxing extraction is added, extracting solution filtering is recycled ethyl alcohol, extracted
Liquid is concentrated into 1.5~2.0g/cm3, be cooled to 0~5 DEG C it is spare.
7. the method according to claim 6 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that the system of coriander extracting solution
Preparation Method are as follows:
Coriander is shredded, the ethyl alcohol of 2 times of its weight, refluxing extraction 2h is added, ethyl alcohol, extracting solution concentration are recycled in extracting solution filtering
To 1.5g/cm3, be cooled to 4 DEG C it is spare.
8. the method according to claim 1 for improving cymose buckwheat rhizome percentage of seedgermination, which is characterized in that in step 1), disinfection
Method be 30min in the liquor potassic permanganate that seed is immersed in mass concentration and is 3%.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110313267A (en) * | 2019-07-29 | 2019-10-11 | 佛山科学技术学院 | A method of promoting bitter buckwheat seed germination |
| RU2775314C1 (en) * | 2021-11-08 | 2022-06-29 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр агробиотехнологий Дальнего Востока им. А.К. Чайки" (ФГБНУ "ФНЦ агробиотехнологий Дальнего Востока им. А.К. Чайки") | Method for presowing seed treatment of spring vetch |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110313267A (en) * | 2019-07-29 | 2019-10-11 | 佛山科学技术学院 | A method of promoting bitter buckwheat seed germination |
| RU2775314C1 (en) * | 2021-11-08 | 2022-06-29 | Федеральное государственное бюджетное научное учреждение "Федеральный научный центр агробиотехнологий Дальнего Востока им. А.К. Чайки" (ФГБНУ "ФНЦ агробиотехнологий Дальнего Востока им. А.К. Чайки") | Method for presowing seed treatment of spring vetch |
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