CN109196124A - 同时检测及定量分析四种血源性病毒的多重Taqman探针qPCR的试剂盒及方法 - Google Patents
同时检测及定量分析四种血源性病毒的多重Taqman探针qPCR的试剂盒及方法 Download PDFInfo
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- CN109196124A CN109196124A CN201680084986.2A CN201680084986A CN109196124A CN 109196124 A CN109196124 A CN 109196124A CN 201680084986 A CN201680084986 A CN 201680084986A CN 109196124 A CN109196124 A CN 109196124A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
Abstract
一种用于同时检测及定量分析四种血源性病毒的多重Taqman探针qPCR的试剂盒及方法。其中该试剂盒包含SEQ ID NO:1、SEQ ID NO:2、与SEQ ID NO:3所组成的组;SEQ ID NO:4、SEQ ID NO:5、与SEQ ID NO:6所组成的组;SEQ ID NO:7、SEQ ID NO:8、与SEQ ID NO:9所组成的组;以及SEQ ID NO:10、SEQ ID NO:11与SEQ ID NO:12所组成的组。目标检测的四种血源性病毒为乙型肝炎病毒(HBV)、丙型肝炎病毒(HCV)、人类免疫缺陷病毒1型(HIV1)、以及人类T细胞淋巴瘤/白血病1型(HTLV1)。
Description
PCT国内申请,说明书已公开。
Claims (11)
- PCT国内申请,权利要求书已公开。
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PCT/CN2016/096964 WO2018035860A1 (zh) | 2016-08-26 | 2016-08-26 | 同时检测及定量分析四种血源性病毒的多重Taqman探针qPCR的试剂盒及方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111286557A (zh) * | 2020-01-16 | 2020-06-16 | 山西国际旅行卫生保健中心(太原海关口岸门诊部) | 一种检测输血性传播病原体的试剂组合、试剂盒及应用 |
CN111926118A (zh) * | 2020-08-21 | 2020-11-13 | 苏州育德扬生物技术有限公司 | 一种可同时检测艾滋病毒1、2型病毒以及人类t淋巴细胞白血病病毒1、2型的检测方法 |
CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
CN116694822A (zh) * | 2023-06-16 | 2023-09-05 | 中山市中心血站 | 实时荧光环介导恒温扩增检测人类嗜t淋巴细胞i型病毒的方法及试剂盒 |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111206123B (zh) * | 2020-04-21 | 2020-07-24 | 珠海丽珠试剂股份有限公司 | 用于血液筛查的核酸组合物和试剂盒 |
CN113234866B (zh) * | 2021-06-30 | 2023-11-14 | 上海君远生物科技有限公司 | 一种同步检测多项血液循环系统病原体的检测试剂盒及其检测方法 |
CN115948617A (zh) * | 2022-12-23 | 2023-04-11 | 圣湘生物科技股份有限公司 | 检测血源性传播病毒的组合物、试剂盒、方法及其用途 |
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CN100475976C (zh) * | 2006-08-18 | 2009-04-08 | 上海科华生物工程股份有限公司 | 一种同步扩增检测肝炎及艾滋病毒核酸的试剂盒 |
CN103184297A (zh) * | 2011-12-27 | 2013-07-03 | 上海复星医学科技发展有限公司 | 一种荧光同步检测肝炎及艾滋病毒核酸的方法及试剂盒 |
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2016
- 2016-08-26 CN CN201680084986.2A patent/CN109196124A/zh active Pending
- 2016-08-26 WO PCT/CN2016/096964 patent/WO2018035860A1/zh active Application Filing
Patent Citations (2)
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CN103088150A (zh) * | 2011-10-28 | 2013-05-08 | 台湾糖业股份有限公司 | 同时检测四种病毒的引物、引物对、方法及试剂盒 |
CN105525035A (zh) * | 2014-09-29 | 2016-04-27 | 天津华大基因科技有限公司 | 血液筛查试剂盒、方法及装置 |
Non-Patent Citations (4)
Title |
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AXELLE DEHÉE ET AL.: "Quantitation of HTLV-Ⅰ proviral load by a TaqMan real-time PCR assay,Axelle Dehée et al.,Journal of virological methods", 《JOURNAL OF VIROLOGICAL METHODS》 * |
MASOOD ZIAEE ET AL.: "The prevalence of HTLV-1 and its co-infection with HCV,HBV and HIV in hemophilic patients", 《PAK J MED SCI》 * |
李桂民: "HBV、HCV、HIV-1荧光定量PCR检测体系的建立和应用", 《中国博士学位论文全文数据库医药卫生科技辑》 * |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111286557A (zh) * | 2020-01-16 | 2020-06-16 | 山西国际旅行卫生保健中心(太原海关口岸门诊部) | 一种检测输血性传播病原体的试剂组合、试剂盒及应用 |
CN111286557B (zh) * | 2020-01-16 | 2023-12-08 | 山西国际旅行卫生保健中心(太原海关口岸门诊部) | 一种检测输血性传播病原体的试剂组合、试剂盒及应用 |
CN111926118A (zh) * | 2020-08-21 | 2020-11-13 | 苏州育德扬生物技术有限公司 | 一种可同时检测艾滋病毒1、2型病毒以及人类t淋巴细胞白血病病毒1、2型的检测方法 |
CN114369649A (zh) * | 2022-02-08 | 2022-04-19 | 山东见微生物科技有限公司 | 一种特异的选择扩增及多重pcr方法和应用 |
CN116694822A (zh) * | 2023-06-16 | 2023-09-05 | 中山市中心血站 | 实时荧光环介导恒温扩增检测人类嗜t淋巴细胞i型病毒的方法及试剂盒 |
CN116694822B (zh) * | 2023-06-16 | 2024-01-30 | 中山市中心血站 | 实时荧光环介导恒温扩增检测人类嗜t淋巴细胞i型病毒的方法及试剂盒 |
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