CN109182142B - 皮落青霉及其应用 - Google Patents
皮落青霉及其应用 Download PDFInfo
- Publication number
- CN109182142B CN109182142B CN201811136587.3A CN201811136587A CN109182142B CN 109182142 B CN109182142 B CN 109182142B CN 201811136587 A CN201811136587 A CN 201811136587A CN 109182142 B CN109182142 B CN 109182142B
- Authority
- CN
- China
- Prior art keywords
- penicillium
- lovastatin
- strain
- fermentation
- dermatum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000228143 Penicillium Species 0.000 title claims abstract description 84
- 229960004844 lovastatin Drugs 0.000 claims abstract description 66
- PCZOHLXUXFIOCF-UHFFFAOYSA-N Monacolin X Natural products C12C(OC(=O)C(C)CC)CC(C)C=C2C=CC(C)C1CCC1CC(O)CC(=O)O1 PCZOHLXUXFIOCF-UHFFFAOYSA-N 0.000 claims abstract description 64
- PCZOHLXUXFIOCF-BXMDZJJMSA-N lovastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)[C@@H](C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 PCZOHLXUXFIOCF-BXMDZJJMSA-N 0.000 claims abstract description 64
- QLJODMDSTUBWDW-UHFFFAOYSA-N lovastatin hydroxy acid Natural products C1=CC(C)C(CCC(O)CC(O)CC(O)=O)C2C(OC(=O)C(C)CC)CC(C)C=C21 QLJODMDSTUBWDW-UHFFFAOYSA-N 0.000 claims abstract description 64
- 238000000855 fermentation Methods 0.000 claims abstract description 61
- 230000004151 fermentation Effects 0.000 claims abstract description 61
- 241001507662 Penicillium crustosum Species 0.000 claims abstract description 15
- 238000004519 manufacturing process Methods 0.000 claims abstract description 10
- 241000228150 Penicillium chrysogenum Species 0.000 claims description 5
- 239000007788 liquid Substances 0.000 abstract description 19
- 238000004321 preservation Methods 0.000 abstract description 7
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 239000000243 solution Substances 0.000 description 19
- 239000001963 growth medium Substances 0.000 description 18
- 238000000034 method Methods 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- 238000011218 seed culture Methods 0.000 description 11
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 238000012258 culturing Methods 0.000 description 9
- 238000004128 high performance liquid chromatography Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 7
- 235000010633 broth Nutrition 0.000 description 7
- 239000008103 glucose Substances 0.000 description 7
- 239000002689 soil Substances 0.000 description 7
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 6
- 241000233866 Fungi Species 0.000 description 6
- 244000061456 Solanum tuberosum Species 0.000 description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 description 6
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 6
- 238000012216 screening Methods 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 238000004809 thin layer chromatography Methods 0.000 description 5
- 235000015278 beef Nutrition 0.000 description 4
- 238000000589 high-performance liquid chromatography-mass spectrometry Methods 0.000 description 4
- 230000000877 morphologic effect Effects 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 229940121710 HMGCoA reductase inhibitor Drugs 0.000 description 3
- 235000012000 cholesterol Nutrition 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000004895 liquid chromatography mass spectrometry Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000228212 Aspergillus Species 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000228347 Monascus <ascomycete fungus> Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 230000004913 activation Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 238000001819 mass spectrum Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000010355 oscillation Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000003505 terpenes Chemical class 0.000 description 2
- -1 vegetation Substances 0.000 description 2
- HSHOXDTWEUKPND-UHFFFAOYSA-N 5-methoxy-5-oxopentaneperoxoic acid Chemical compound COC(=O)CCCC(=O)OO HSHOXDTWEUKPND-UHFFFAOYSA-N 0.000 description 1
- 241000203233 Aspergillus versicolor Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 241001247197 Cephalocarida Species 0.000 description 1
- 244000037364 Cinnamomum aromaticum Species 0.000 description 1
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 description 1
- RGJOEKWQDUBAIZ-IBOSZNHHSA-N CoASH Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCS)O[C@H]1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-IBOSZNHHSA-N 0.000 description 1
- 244000133098 Echinacea angustifolia Species 0.000 description 1
- 241000726221 Gemma Species 0.000 description 1
- 206010069767 H1N1 influenza Diseases 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 240000002262 Litsea cubeba Species 0.000 description 1
- 235000012854 Litsea cubeba Nutrition 0.000 description 1
- 241000123069 Ocyurus chrysurus Species 0.000 description 1
- 229930182555 Penicillin Natural products 0.000 description 1
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 1
- 241000228129 Penicillium janthinellum Species 0.000 description 1
- 240000002044 Rhizophora apiculata Species 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- WYTGDNHDOZPMIW-RCBQFDQVSA-N alstonine Chemical class C1=CC2=C3C=CC=CC3=NC2=C2N1C[C@H]1[C@H](C)OC=C(C(=O)OC)[C@H]1C2 WYTGDNHDOZPMIW-RCBQFDQVSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- RGJOEKWQDUBAIZ-UHFFFAOYSA-N coenzime A Natural products OC1C(OP(O)(O)=O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 RGJOEKWQDUBAIZ-UHFFFAOYSA-N 0.000 description 1
- 239000005516 coenzyme A Substances 0.000 description 1
- 229940093530 coenzyme a Drugs 0.000 description 1
- KDTSHFARGAKYJN-UHFFFAOYSA-N dephosphocoenzyme A Natural products OC1C(O)C(COP(O)(=O)OP(O)(=O)OCC(C)(C)C(O)C(=O)NCCC(=O)NCCS)OC1N1C2=NC=NC(N)=C2N=C1 KDTSHFARGAKYJN-UHFFFAOYSA-N 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000014134 echinacea Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000012262 fermentative production Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000002471 hydroxymethylglutaryl coenzyme A reductase inhibitor Substances 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000006799 invasive growth in response to glucose limitation Effects 0.000 description 1
- 125000003151 isocoumarinyl group Chemical class C1(=O)OC(=CC2=CC=CC=C12)* 0.000 description 1
- 230000001665 lethal effect Effects 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229940049954 penicillin Drugs 0.000 description 1
- 210000000557 podocyte Anatomy 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 229930004725 sesquiterpene Natural products 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 201000010740 swine influenza Diseases 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 241000712461 unidentified influenza virus Species 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
Abstract
Description
Claims (3)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811136587.3A CN109182142B (zh) | 2018-09-28 | 2018-09-28 | 皮落青霉及其应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201811136587.3A CN109182142B (zh) | 2018-09-28 | 2018-09-28 | 皮落青霉及其应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN109182142A CN109182142A (zh) | 2019-01-11 |
CN109182142B true CN109182142B (zh) | 2020-11-03 |
Family
ID=64907550
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201811136587.3A Active CN109182142B (zh) | 2018-09-28 | 2018-09-28 | 皮落青霉及其应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN109182142B (zh) |
Families Citing this family (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110684669B (zh) * | 2019-03-07 | 2023-05-30 | 慕恩(广州)生物科技有限公司 | 一株壳青霉及其应用 |
Family Cites Families (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1953235A3 (en) * | 2006-05-17 | 2008-11-19 | Metanomics GmbH | New genes related to a process for the production of fine chemicals |
CN106479900B (zh) * | 2016-09-30 | 2019-04-16 | 浙江师范大学 | 高产洛伐他汀草酸青霉Po-25菌株及其用途 |
CN108315265B (zh) * | 2017-12-28 | 2020-12-04 | 浙江师范大学 | 一种杂色曲霉Av-2菌株及其应用 |
-
2018
- 2018-09-28 CN CN201811136587.3A patent/CN109182142B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN109182142A (zh) | 2019-01-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN101445784B (zh) | 布雷正青霉变种zjb082702及其发酵制备布雷菲德菌素a的应用 | |
CN105062895B (zh) | 一种高产胞外黄色素的红曲霉菌株及其选育方法与应用 | |
CN106978350A (zh) | 一株黑曲霉及其在普洱茶素类化合物的制备中的应用 | |
Kumar et al. | Cultural, morphological and molecular characterization of vinca alkaloids producing endophytic fungus Fusarium solani isolated from Catharanthus roseus | |
CN106010980B (zh) | 一种内生真菌巴西类壳小圆孢菌株及其应用 | |
CN1076965A (zh) | 新的真菌菌株及其在抗生素生产中的应用 | |
US20230295675A1 (en) | Endophytic bacterial strain with high camptothecin yield and use thereof | |
CN108315265B (zh) | 一种杂色曲霉Av-2菌株及其应用 | |
EP3029147A1 (en) | A method of semi-solid state fermentation for producing surfactin from a mutant strain of bacillus subtilis subsp | |
CN105219653B (zh) | 产洛伐他汀的棒曲霉(Aspergillus clavatus)Ac-32菌株及其应用 | |
CN105018352A (zh) | 一种产曲酸真菌菌株及制备方法 | |
CN109182142B (zh) | 皮落青霉及其应用 | |
CN108841889B (zh) | 利用微生物发酵生产松刚霉素主份——灰黄霉素的方法 | |
CN104726347A (zh) | 一株狐粪青霉真菌菌株以及利用该菌株制备左旋7-羟基丁苯酞的方法 | |
CN106479900B (zh) | 高产洛伐他汀草酸青霉Po-25菌株及其用途 | |
CN103981104A (zh) | 一株内生真菌及其生物转化甘草酸为甘草次苷的方法 | |
CN103724290A (zh) | 一种环肽化合物clavatustide A及其产生菌、制备方法和应用 | |
CN113481106B (zh) | 一种深海来源蕈青霉及获得化合物 | |
CN113337432B (zh) | 一种产吡咯喹啉醌的食甲基菌及其应用 | |
CN106754430A (zh) | 高产洛伐他汀枝状枝孢菌Cc‑106菌株及其用途 | |
CN105602856B (zh) | 黑曲霉(Aspergillus niger)An-19菌株及其用于洛伐他汀的生产的用途和发酵方法 | |
WO2005063963A1 (en) | Microorganism producing tacrolimus and mass-productive method tacrolimus using the same | |
CN106434366B (zh) | 高产洛伐他汀米曲霉Ao-57菌株和用途 | |
CN103805543B (zh) | 一种产除莠霉素的菌株及其应用 | |
CN101486974B (zh) | 一种产紫杉醇的内生真菌 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240131 Address after: 250000 Hisense Tianchen, Tianchen Road, Jinan City, Shandong Province Patentee after: Zhuofan (Jinan) Technology Innovation Co.,Ltd. Country or region after: China Address before: 321004 No. 688 Yingbin Avenue, Wucheng District, Zhejiang, Jinhua Patentee before: ZHEJIANG NORMAL University Country or region before: China |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20240205 Address after: 710100 Building 6, No. 381 Kunchi 1st Road, Xiliu Street Office, High tech Zone, Xi'an City, Shaanxi Province Patentee after: Shaanxi Didu Pharmaceutical and Chemical Co.,Ltd. Country or region after: China Address before: 250000 Hisense Tianchen, Tianchen Road, Jinan City, Shandong Province Patentee before: Zhuofan (Jinan) Technology Innovation Co.,Ltd. Country or region before: China |