CN109172481B

CN109172481B - Female hip treatment and protection essence

Info

Publication number: CN109172481B
Application number: CN201811242083.XA
Authority: CN
Inventors: 任娟
Original assignee: Xi'an Weiyi Biotechnology Co ltd
Current assignee: Xi'an Weiyi Biotechnology Co ltd
Priority date: 2018-10-24
Filing date: 2018-10-24
Publication date: 2021-05-11
Anticipated expiration: 2038-10-24
Also published as: CN109172481A

Abstract

The invention discloses a female hip therapy hip-care essence which comprises the following components: water, corn sprout extract, ferulic acid, glycerol, honey, ostrich oil, carbomer, sargassum fusiforme polysaccharide and glucoside. The female hip-therapy hip-care essence disclosed by the invention has good stability, is capable of relieving and moisturizing, improving the elasticity and smoothness of skin, maintaining the health of the skin, and preventing various external stimuli, so that the purpose of moistening and beautifying the skin is achieved. In addition, the skin care product can resist oxidation, regulate the immunologic function, promote the generation of collagen, improve the skin repair environment and have remarkable promoting effect on the epithelial cells of the buttocks.

Description

Female hip treatment and protection essence

Technical Field

The invention relates to the technical field of skin care products, in particular to a female hip therapy hip protection essence.

Background

With the aging, the skin ages more and more rapidly, the skin of a common female tends to slide down at the age of 25 years, the skin begins to become loose and hyperpigmented, and the basic moisturizing cream cannot meet the skin requirement, and only can improve the skin by supplementing essence. In order to achieve good effects, the existing essence uses various anti-aging substances, and although the skin color can be improved within a certain time, the skin and even the body can be damaged after long-term use.

The invention of application No. 201610858160.9 discloses a jade muscle essence lotion, which is composed of the following raw materials in parts by weight: 65-80 parts of deionized water, 0.01-0.1 part of EDTA disodium, 0.8-0.8 part of carbomer 9410.2, 1-10 parts of glycerol, 1-10 parts of propylene glycol, 0.05-0.3 part of sodium hyaluronate, 0.2-0.8 part of triethanolamine, 3-8 parts of liquorice extract, 0.5-2 parts of soluble pearl powder, 0.5-2 parts of motherwort extract, 0.5-2 parts of asarum extract, 1.0-5.0 parts of radix angelicae extract, 0.8-4 parts of bletilla striata extract, 0.1-0.8 part of anti-allergy agent and 0.1-0.8 part of preservative. Has effects of nourishing facial skin, regulating skin nutrition and moisture, and whitening skin, and especially has special improvement effect on facial mottle, tarnish, withered and yellow, laxity, dryness, and no wonderful symptoms.

The invention of application No. 201510900305.2 particularly relates to a cutin repair essence lotion and a preparation method thereof, wherein the essence lotion comprises artemisia capillaris flower extract, citrus unshiu peel extract, orange fruit extract, clove flower extract and plum fruit extract. The moisturizing component is obtained by screening, and the moisturizing component is added into a cosmetic matrix, so that the moisturizing cream has a good moisturizing effect on skin; in addition, arginine is added into the essence to ensure the stability of the essence.

The invention of application number 201510490348.8 discloses a repairing and moistening essence which is prepared from the following raw materials in parts by weight: 30-50 parts of deionized water, 3-6 parts of butanediol, 2-5 parts of lactobacillus leavening, 2-6 parts of squalane, 2-4 parts of chamomile extract, 1-2 parts of caffeine, 0.2-0.8 part of hydrogenated lecithin, 0.2-0.6 part of hyaluronic acid and 0.1-0.5 part of phenoxyethanol. The repairing and moistening essence ensures the nutrition required by the skin through the humectant and the extract by preferably selecting and proportioning a plurality of skin care substances without adding essence; in addition, caffeine plays a role in accelerating skin blood circulation; the squalane has good skin moistening effect; hydrogenated lecithin is used as an emulsifier and an emollient, and has no irritation; the phenoxyethanol is used as a mild preservative and has small irritation to skin.

The invention of application number 201711013365.8 particularly relates to a moisturizing and soothing essence lotion and a preparation method thereof, and the moisturizing and soothing essence lotion is prepared from the following raw materials: the purslane extract, the tremella extract and the betaine are contained in the cosmetic, so that the cosmetic has moisturizing and relieving effects.

Disclosure of Invention

The invention aims to provide a female hip therapy hip-care essence.

The invention discloses a female hip therapy hip-care essence which comprises the following components: water, corn sprout extract, ferulic acid, glycerol, honey, ostrich oil, carbomer, sargassum fusiforme polysaccharide and glucoside.

As one technical scheme of the invention, the female hip therapy hip-care essence comprises the following raw materials in parts by weight: 50-80 parts of water, 2-4 parts of corn sprout extract, 0.01-0.05 part of ferulic acid, 1-10 parts of glycerol, 0.5-2.5 parts of honey, 1-3 parts of ostrich oil, 0.2-0.6 part of carbomer, 0.2-0.8 part of sargassum fusiforme polysaccharide, 2-3 parts of glucoside and 0.003-0.006 part of hyaluronic acid.

As a second technical scheme of the invention, the female hip-treatment hip-care essence comprises the following raw materials in parts by weight: 50-80 parts of water, 2-4 parts of corn sprout extract, 0.01-0.05 part of ferulic acid, 1-10 parts of glycerol, 0.5-2.5 parts of honey, 1-3 parts of ostrich oil, 0.2-0.6 part of carbomer, 0.2-0.8 part of sargassum fusiforme polysaccharide, 2-3 parts of glucoside and 0.003-0.006 part of agarose graft modified hyaluronic acid.

As a third technical scheme of the invention, the female hip-therapy hip-care essence is prepared from the following raw materials in parts by weight: 50-80 parts of water, 2-7 parts of astaxanthin nano-composite, 2-4 parts of corn sprout extract, 0.01-0.05 part of ferulic acid, 1-10 parts of glycerol, 0.5-2.5 parts of honey, 1-3 parts of ostrich oil, 0.2-0.6 part of carbomer, 0.2-0.8 part of sargassum fusiforme polysaccharide, 2-3 parts of glucoside and 0.003-0.006 part of agarose graft modified hyaluronic acid.

Hyaluronic acid is an important scaffold component in the connective tissue of the skin, having the ability to bind water and good biocompatibility. But hyaluronic acid is easy to degrade, and the invention reduces the degradation speed of hyaluronic acid, promotes the proliferation of fibroblasts and the generation of collagen, and promotes skin traceless repair and wound repair by using agarose modified hyaluronic acid. Therefore, the female hip-therapy hip-care essence disclosed by the invention can deeply penetrate into the epidermis or even the dermis of the skin to play a role in maintaining and enhancing the water absorption capacity and barrier function of the stratum corneum, and the water content of the stratum corneum is maintained, so that the skin is prevented from being dried.

The agarose graft modified hyaluronic acid is obtained by the following method: dissolving 5-15 g of agarose into 500-1500 g of water with the temperature of 50-60 ℃, fully mixing, naturally cooling to 30-40 ℃, and standing for 1-3 hours; adding 2.5-6 g of sodium hydroxide and 12-40 g of epoxy chloropropane, and stirring for 2-5 hours at 30-40 ℃; drying under reduced pressure for 30-60 minutes, and removing redundant epoxy chloropropane to obtain a concentrated solution; adding water with the volume 1-2 times that of the concentrated solution into the concentrated solution, centrifuging for 10-25 minutes, and collecting supernatant; adding 5-15 g of hyaluronic acid into the supernatant, stirring and reacting at 30-40 ℃ for 48-60 hours, and collecting reaction liquid; drying the reaction liquid under reduced pressure for 2-4 hours, and dialyzing for 48-72 hours by using a dialysis bag with the cut-off molecular weight of 8000-12000 to obtain a reserved liquid; and (4) carrying out vacuum freeze drying on the retention solution to obtain the agarose graft modified hyaluronic acid.

Further, the astaxanthin nano-composite is chitosan/lecithin/astaxanthin nano-composite and is prepared by the following method: dissolving 500-1000 mg of astaxanthin in 200-400 mL of 20-30 mg/mL lecithin ethanol solution to obtain solution A; dissolving 250-500 mg of chitosan into 25-50 mL of hydrochloric acid solution with the concentration of 0.2-0.3 mol/L to serve as solution B; injecting the solution B into the solution A, then adding 2000-4000 mL of 1.5-2% by mass of Tween-80 aqueous solution serving as an emulsifier, fully stirring, and performing ultrasonic treatment for 15-30 minutes to obtain a solution C; standing the solution C at 2-4 ℃ for 4-6 hours, dialyzing for 3-6 hours by using a dialysis bag with the molecular weight cutoff of 8000-14000 Da, and taking a retention solution; and (4) carrying out vacuum freeze drying on the retention solution to obtain the chitosan/lecithin/astaxanthin nano compound.

The chitosan belongs to linear macromolecules and can be completely absorbed by organisms, the lecithin has good skin compatibility, and the astaxanthin, the chitosan and the lecithin are compounded, so that the stability of the astaxanthin can be improved, and the astaxanthin can be absorbed and utilized by human bodies more conveniently. The invention introduces the hydrophobic group of lecithin into a chitosan hydrophilic chain under the action of ultrasonic assistance, and forms a chitosan/lecithin/astaxanthin nano compound with a core-shell structure of a hydrophilic shell and a hydrophobic inner core by self-aggregation under the driving forces of hydrophobic action, electrostatic action and the like.

Further, the astaxanthin nano-composite is chitosan/astaxanthin/DNA nano-composite and is prepared by the following method: dissolving 500-1000 mg of chitosan into 40-80 mL of deionized water, and stirring for 2-3 hours to obtain a chitosan hydration solution; adding 40-80 mL of hydrochloric acid solution with the pH of 2 into the chitosan hydration solution, stirring for 2-3 hours, and adjusting the pH to 7 by using sodium hydroxide aqueous solution with the pH of 13; finally, deionized water is added to obtain chitosan mother liquor with the chitosan concentration of 0.02-0.04 mg/mL as solution A; dissolving 100-200 mg of astaxanthin into 150-300 mL of absolute ethyl alcohol, stirring for 24-36 hours in a dark environment, centrifuging for 5-10 minutes, and taking supernatant to obtain an astaxanthin ethanol solution as a solution B; dissolving salmon sperm DNA into deionized water, and preparing to obtain salmon sperm DNA solution with the concentration of 0.02-0.32 mg/mL as solution C; and (3) uniformly mixing the solution A and the solution B under the condition of keeping out of the sun, and adding the solution C, wherein the volume ratio of the solution A to the solution B to the solution C is 2: (0.5-3): 1, fully and uniformly mixing, and carrying out vacuum freeze drying to obtain the chitosan/astaxanthin/DNA nano compound.

The concentration of the salmon sperm DNA solution is preferably 0.02-0.04 mg/mL.

According to the invention, the chitosan and DNA macromolecules form nanoparticles through self-assembly, the hydrophobic micro-region exists in the spatial structure of the nanoparticles, and the hydrophobic micro-region is combined with fat-soluble astaxanthin to wrap astaxanthin, so that the water solubility and stability of the astaxanthin are further improved, and the activity and bioavailability of the astaxanthin are improved. Wherein, when the salmon sperm DNA solution is 0.02mg/mL, the chitosan/astaxanthin/DNA nano compound is bonded most tightly among the molecules, and the oxidation resistance and the stability are strongest.

The deacetylation degree of the chitosan is 90-95%.

The glucoside is polyethylene glycol starch glucoside and/or polyethylene glycol glucoside. Preferably, the glucoside is polyethylene glycol starch glucoside and polyethylene glycol glucoside in a mass ratio of 1: 1, in a mixture of the components.

The Mel is one or more of Japanese pagodatree flower honey, all flowers honey, winter honey, Astragalus honey, litchi honey, linden honey, flos Sophorae honey, flos Osmanthi Fragrantis honey, Coptidis rhizoma honey, Galla chinensis honey, Saviae Miltiorrhizae radix honey, radix astragali honey, Galla chinensis honey, herba Leonuri honey, and fructus Lycii honey.

The female hip-therapy hip-care essence disclosed by the invention has good stability, is capable of relieving and moisturizing, improving the elasticity and smoothness of skin, maintaining the health of the skin, and preventing various external stimuli, so that the purpose of moistening and beautifying the skin is achieved. In addition, the skin care product can resist oxidation, regulate the immunologic function, promote the generation of collagen, improve the skin repair environment and have remarkable promoting effect on the epithelial cells of the buttocks.

Detailed Description

The raw materials in the examples are as follows:

corn sprout extract, provided by Shanxi pannier Biotech Co., Ltd., 20g of corn sprout was extracted with absolute ethanol to obtain 1g of corn sprout extract.

Ferulic acid, CAS number: 1135-24-6, cosmetic grade, available from Shanghai Fengshi Biotech, Inc.

Glycerin, supplied by Guangdong Cuixin chemical Co., Ltd., cosmetic grade.

Acacia honey is available from Xinyang bee industries, Inc. of Hubei province.

Ostrich oil, offered by the Huamei natural perfume oil refinery of Qingyuan of Jian city.

Carbomer, specifically carbomer 940, available from Hangzhou carbomer import and export Co., Ltd, cosmetic grade.

Sargassum fusiforme polysaccharide, supplied by west ann ran bioengineering limited.

The polyethylene glycol starch glycoside is prepared by referring to synthesis and moisture retention of polyethylene glycol starch glycoside (fine chemical industry, wangqingning, 2012, 05 th), and the reaction conditions are as follows: the reaction temperature is 118 ℃, the reaction time is 4.5 hours, and the mass ratio of the starch to the p-toluenesulfonic acid to the ethylene glycol to the polyethylene glycol 800 is 1: 0.025: 4: 2.

hyaluronic acid, available from Guangzhou republic chemical Limited, molecular weight 5000.

Astaxanthin, available from Shandong McRong bioengineering, Inc.

Lecithin, conceivably offered by international trade (shanghai) ltd, food grade.

Absolute ethanol, food grade, available from billion of reals, zheng.

Tween-80, CAS No.: 9005-65-6, and is food grade.

Chitosan, available from san Lisa Biotech GmbH, Shandong, with a degree of deacetylation of 90%.

And (3) adding 0.96mL of hydrochloric acid with the mass fraction of 38% into 1000mL of hydrochloric acid solution with the pH of 2 to obtain the hydrochloric acid solution with the pH of 2.

As an aqueous solution of sodium hydroxide having a pH of 13, hydrochloric acid having a molar concentration of 0.125mol/L is specifically used.

Salmon sperm DNA, purchased from Sigma.

The polyethylene glycol glucoside is prepared by referring to 'synthesis and moisture retention research of polyethylene glycol glucoside' (daily chemical industry, jin Xin, No. 4 of 2000), and the reaction conditions are as follows: the reaction temperature is 120 ℃, the catalyst dosage is 0.1%, and the ratio of alcohol to sugar is 1: 1.

agarose, supplied by Zhengzhou Yuzheng food additives Co., Ltd., food grade.

Sodium hydroxide, CAS No.: 1310-73-2.

Epichlorohydrin, CAS No.: 106-89-8.

In the case where no specific description is given in the examples, the specific process conditions for vacuum freeze-drying are as follows: the pre-freezing temperature is-80 ℃, the pre-freezing time is 2 hours, the freezing temperature is-60 ℃, the freezing time is 48 hours, and the absolute pressure is 100 Pa.

Example 1

The female hip treatment hip-protection essence is prepared from the following raw materials in parts by weight: 70 parts of deionized water, 4 parts of corn sprout extract, 0.02 part of ferulic acid, 10 parts of glycerol, 2 parts of acacia honey, 3 parts of ostrich oil, 0.5 part of carbomer, 0.2 part of sargassum fusiforme polysaccharide, 3 parts of polyethylene glycol starch glycoside and 0.004 part of hyaluronic acid.

The preparation method of the female hip-treatment hip-protection essence comprises the following steps: adding deionized water, glycerol, carbomer and polyethylene glycol starch glucoside into a water bath, heating to 80 ℃, and stirring at 800 revolutions per minute for 30 minutes; stopping stirring, cooling to 40 deg.C, sequentially adding corn sprout extract, ferulic acid, locust honey, ostrich oil, Cyrtymenia Sparsa polysaccharide, and hyaluronic acid, and stirring at 300 rpm for 20 min; and finally, cooling to 30 ℃ to obtain the female hip treatment and protection essence.

Example 2

The female hip treatment hip-protection essence is prepared from the following raw materials in parts by weight: 70 parts of deionized water, 4 parts of corn sprout extract, 0.02 part of ferulic acid, 10 parts of glycerol, 2 parts of acacia honey, 3 parts of ostrich oil, 0.5 part of carbomer, 0.2 part of sargassum fusiforme polysaccharide, 3 parts of polyethylene glycol starch glycoside and 0.004 part of agarose graft modified hyaluronic acid.

The preparation method of the female hip-treatment hip-protection essence comprises the following steps: adding deionized water, glycerol, carbomer and polyethylene glycol starch glucoside into a water bath, heating to 80 ℃, and stirring at 800 revolutions per minute for 30 minutes; stopping stirring, cooling to 40 deg.C, sequentially adding corn sprout extract, ferulic acid, locust honey, ostrich oil, Cyrtymenia Sparsa polysaccharide, and agarose graft modified hyaluronic acid, and stirring at 300 r/min for 20 min; and finally, cooling to 30 ℃ to obtain the female hip treatment and protection essence.

The agarose graft modified hyaluronic acid is obtained by the following method: dissolving 5g of agarose in 500g of water with the temperature of 60 ℃, fully and uniformly mixing, naturally cooling to 30 ℃, and standing for 1 hour; adding 2.5g of sodium hydroxide and 12g of epoxy chloropropane, and stirring at 30 ℃ at 80 rpm for 3 hours; drying under reduced pressure at 55 deg.C and 0.04MPa for 30 min to remove excessive epichlorohydrin and obtain concentrated solution; adding deionized water with the same volume as the concentrated solution into the concentrated solution, centrifuging at 4000 rpm for 10 minutes, and collecting supernatant; adding 5g hyaluronic acid into the supernatant, reacting at 30 deg.C under stirring at 80 rpm for 48 hr, and collecting the reaction solution; drying the reaction solution at 60 deg.C under 0.04MPa for 4 hr, and dialyzing with dialysis bag with cut-off molecular weight of 12000 for 72 hr to obtain a retention solution; and (4) carrying out vacuum freeze drying on the retention solution to obtain the agarose graft modified hyaluronic acid.

Example 3

The female hip treatment hip-protection essence is prepared from the following raw materials in parts by weight: 70 parts of deionized water, 5 parts of astaxanthin nano-composite, 4 parts of corn sprout extract, 0.02 part of ferulic acid, 10 parts of glycerol, 2 parts of acacia honey, 3 parts of ostrich oil, 0.5 part of carbomer, 0.2 part of sargassum fusiforme polysaccharide, 3 parts of polyethylene glycol starch glycoside and 0.004 part of agarose graft modified hyaluronic acid.

The preparation method of the female hip-treatment hip-protection essence comprises the following steps: adding deionized water, glycerol, carbomer and polyethylene glycol starch glucoside into a water bath, heating to 80 ℃, and stirring at 800 revolutions per minute for 30 minutes; stopping stirring, cooling to 40 deg.C, sequentially adding astaxanthin nanometer complex, corn sprout extract, ferulic acid, locust honey, ostrich oil, Cyrtymenia Sparsa polysaccharide, and agarose graft-modified hyaluronic acid, and stirring at 300 rpm for 20 min; and finally, cooling to 30 ℃ to obtain the female hip treatment and protection essence.

The astaxanthin nano-composite is chitosan/lecithin/astaxanthin nano-composite and is prepared by the following method: dissolving 500mg of astaxanthin in 200mL of lecithin ethanol solution with the concentration of 25mg/mL to obtain solution A; dissolving 250mg of chitosan into 25mL of hydrochloric acid solution with the concentration of 0.27mol/L to obtain solution B; injecting the solution B into the solution A by using a sterile syringe with a needle head diameter of 0.7mm, then adding 2000mL of 1.8 mass percent Tween-80 aqueous solution as an emulsifier, fully stirring, and performing ultrasonic treatment under the condition of ultrasonic power of 300W for 16 minutes to obtain solution C; standing the solution C at 4 deg.C for 6 hr, dialyzing with dialysis bag with cut-off molecular weight of 12000Da for 4 hr, and collecting the remaining solution; and (4) carrying out vacuum freeze drying on the retention solution to obtain the chitosan/lecithin/astaxanthin nano compound.

Example 4

The astaxanthin nano-composite is a chitosan/astaxanthin/DNA nano-composite and is prepared by the following method: dissolving 500mg of chitosan into 40mL of deionized water, and stirring for 2 hours at 300 revolutions per minute to obtain a chitosan hydration solution; 40mL of hydrochloric acid solution with the pH value of 2 is dropwise added into the chitosan hydration solution at the speed of 50mL/h, and after stirring for 2 hours at 500 revolutions per minute, the pH value is adjusted to 7 by using sodium hydroxide aqueous solution with the pH value of 13; finally, deionized water is added to obtain chitosan mother liquor with the chitosan concentration of 0.02mg/mL as solution A; dissolving 100mg of astaxanthin into 180mL of absolute ethyl alcohol, stirring for 24 hours at 500 revolutions per minute in a dark environment, centrifuging for 5 minutes at high speed at 12000 revolutions per minute, and taking supernatant to obtain an astaxanthin ethyl alcohol solution as solution B; dissolving salmon sperm DNA into deionized water to prepare salmon sperm DNA solution with the concentration of 0.02mg/mL as solution C; uniformly mixing the solution A and the solution B under the condition of keeping out of the sun, and dropwise adding the solution C at the speed of 50mL/h, wherein the volume ratio of the solution A to the solution B to the solution C is 2: 1: 1, fully and uniformly mixing, and carrying out vacuum freeze drying to obtain the chitosan/astaxanthin/DNA nano compound.

Example 5

The astaxanthin nano-composite is a chitosan/astaxanthin/DNA nano-composite and is prepared by the following method: dissolving 500mg of chitosan into 40mL of deionized water, and stirring for 2 hours at 300 revolutions per minute to obtain a chitosan hydration solution; 40mL of hydrochloric acid solution with the pH value of 2 is dropwise added into the chitosan hydration solution at the speed of 50mL/h, and after stirring for 2 hours at 500 revolutions per minute, the pH value is adjusted to 7 by using sodium hydroxide aqueous solution with the pH value of 13; finally, deionized water is added to obtain chitosan mother liquor with the chitosan concentration of 0.02mg/mL as solution A; dissolving 100mg of astaxanthin into 180mL of absolute ethyl alcohol, stirring for 24 hours at 500 revolutions per minute in a dark environment, centrifuging for 5 minutes at high speed at 12000 revolutions per minute, and taking supernatant to obtain an astaxanthin ethyl alcohol solution as solution B; dissolving salmon sperm DNA into deionized water to prepare salmon sperm DNA solution with the concentration of 0.08mg/mL as solution C; uniformly mixing the solution A and the solution B under the condition of keeping out of the sun, and dropwise adding the solution C at the speed of 50mL/h, wherein the volume ratio of the solution A to the solution B to the solution C is 2: 1: 1, fully and uniformly mixing, and carrying out vacuum freeze drying to obtain the chitosan/astaxanthin/DNA nano compound.

Example 6

The astaxanthin nano-composite is a chitosan/astaxanthin/DNA nano-composite and is prepared by the following method: dissolving 500mg of chitosan into 40mL of deionized water, and stirring for 2 hours at 300 revolutions per minute to obtain a chitosan hydration solution; 40mL of hydrochloric acid solution with the pH value of 2 is dropwise added into the chitosan hydration solution at the speed of 50mL/h, and after stirring for 2 hours at 500 revolutions per minute, the pH value is adjusted to 7 by using sodium hydroxide aqueous solution with the pH value of 13; finally, deionized water is added to obtain chitosan mother liquor with the chitosan concentration of 0.02mg/mL as solution A; dissolving 100mg of astaxanthin into 180mL of absolute ethyl alcohol, stirring for 24 hours at 500 revolutions per minute in a dark environment, centrifuging for 5 minutes at high speed at 12000 revolutions per minute, and taking supernatant to obtain an astaxanthin ethyl alcohol solution as solution B; dissolving salmon sperm DNA into deionized water to prepare salmon sperm DNA solution with the concentration of 0.16mg/mL as solution C; uniformly mixing the solution A and the solution B under the condition of keeping out of the sun, and dropwise adding the solution C at the speed of 50mL/h, wherein the volume ratio of the solution A to the solution B to the solution C is 2: 1: 1, fully and uniformly mixing, and carrying out vacuum freeze drying to obtain the chitosan/astaxanthin/DNA nano compound.

Example 7

The female hip treatment hip-protection essence is prepared from the following raw materials in parts by weight: 70 parts of deionized water, 5 parts of astaxanthin nano-composite, 4 parts of corn sprout extract, 0.02 part of ferulic acid, 10 parts of glycerol, 2 parts of acacia honey, 3 parts of ostrich oil, 0.5 part of carbomer, 0.2 part of sargassum fusiforme polysaccharide, 3 parts of polyethylene glycol glucoside and 0.004 part of agarose graft modified hyaluronic acid.

The preparation method of the female hip-treatment hip-protection essence comprises the following steps: adding deionized water, glycerol, carbomer and polyethylene glycol glucoside into a water bath, heating to 80 ℃, and stirring at 800 revolutions per minute for 30 minutes; stopping stirring, cooling to 40 deg.C, sequentially adding astaxanthin nanometer complex, corn sprout extract, ferulic acid, locust honey, ostrich oil, Cyrtymenia Sparsa polysaccharide, and agarose graft-modified hyaluronic acid, and stirring at 300 rpm for 20 min; and finally, cooling to 30 ℃ to obtain the female hip treatment and protection essence.

Example 8

The female hip treatment hip-protection essence is prepared from the following raw materials in parts by weight: 70 parts of deionized water, 5 parts of astaxanthin nano-composite, 4 parts of corn sprout extract, 0.02 part of ferulic acid, 10 parts of glycerol, 2 parts of acacia honey, 3 parts of ostrich oil, 0.5 part of carbomer, 0.2 part of sargassum fusiforme polysaccharide, 1.5 parts of polyethylene glycol starch glycoside, 1.5 parts of polyethylene glycol glucoside and 0.004 part of agarose graft-modified hyaluronic acid.

The preparation method of the female hip-treatment hip-protection essence comprises the following steps: adding deionized water, glycerol, carbomer, polyethylene glycol starch glucoside and polyethylene glycol glucoside into a water bath, heating to 80 ℃, and stirring at 800 revolutions per minute for 30 minutes; stopping stirring, cooling to 40 deg.C, sequentially adding astaxanthin nanometer complex, corn sprout extract, ferulic acid, locust honey, ostrich oil, Cyrtymenia Sparsa polysaccharide, and agarose graft-modified hyaluronic acid, and stirring at 300 rpm for 20 min; and finally, cooling to 30 ℃ to obtain the female hip treatment and protection essence.

Effect example 1

The safety performance of the hip-care essence for hip therapy of women in examples 1-8 was evaluated.

Test animals: new Zealand white rabbits are common-grade, 2.4-3.0 kg in weight and are half female and half male.

Acute skin irritation test method

New Zealand white rabbits are randomly divided into two groups, namely a complete skin group and a damaged skin group, and the male and female parts are respectively half. 24 hours before the test, the two sides of the dorsal spine of the New Zealand white rabbit are divided into 2 blocks from left to right, the unhairing range of each block is 3cm multiplied by 6cm, and the skin cannot be injured. The damaged skin group was scratched with a sterilized needle at the depilated site for 2cm × 2cm of a # -shaped scar, and attention was paid to scratching only the epidermis without damaging the dermis to a degree of mild bleeding. The test uses self left and right controls. Before application, the hair area is sterilized with medical alcohol, 3mL of the test substance is applied to the skin, the left side is coated with the test substance, and the right side is coated with an equal amount of physiological saline as a control. Each New Zealand white rabbit was raised in one cage, and the residual test substance was removed with warm water 24 hours after application. The skin reaction of the applied part was observed at 1 hour, 24 hours and 48 hours after the removal of the test substance, and skin reaction integration and stimulation intensity evaluation were performed according to the literature. The specific operation and evaluation are referred to GB7919-87 cosmetic safety evaluation program and method, and toxicology foundation (Jintai 24297, Shanghai Compound denier university Press, 2003: 248-.

The results of the acute skin irritation test are shown in table 1.

TABLE 1 acute skin irritation test results

According to the GB7919-87 stimulation intensity grading standard: the integral value is 0-0.4 nonirritant, the light irritancy is 0.5-1.9, the moderate irritancy is 2.0-5.9, and the strong irritancy is 6.0-8.0.

(II) multiple skin irritation test method

New Zealand white rabbits were taken and grouped and subjected to the same skin smearing test. The test substance 3mL is smeared on the hair removal area on the left side of the skin, covered by a layer of oilpaper and fixed by a non-irritant adhesive plaster and a bandage. The right unhairing zone was treated with saline as a control, 2 times daily for 14 days. Shearing hair before applying medicine every time, and the skin should not be damaged. Skin reactions were observed daily and skin reaction integrals were performed as per literature. The specific operation and evaluation are referred to GB7919-87 cosmetic safety evaluation program and method, and toxicology foundation (Jintai 24297, Shanghai Compound denier university Press, 2003: 248-.

The results of the multiple skin irritation tests are shown in table 2.

TABLE 2 results of multiple skin irritation tests

According to the GB7919-87 stimulation intensity grading standard: the integral value is 0-0.4 nonirritant, 0.5-1.9 light irritant, 2.0-5.9 medium irritant and 6.0-8.0 strong irritant. The female hip-treatment hip-care essence disclosed by the invention has no irritation to skin. Moreover, observation shows that after the buttocks-treatment and buttocks-protection essence lotion is smeared on new zealand rabbits with complete skin groups, the skin has no red swelling and edema phenomenon, and the weight and diet are normal.

Effect example 2

The female hip therapy hip-care essence lotions of examples 1 to 8 were evaluated for skin healing promoting performance.

Test animals: kunming mice, 6-8 weeks, spf grade, supplied by Beijing Wittingerihua laboratory animals Co.

Kunming mice were randomized to experimental and blank control groups, respectively. 3% sodium pentobarbital (80mg/kg) normal saline is injected into the abdominal cavity for anesthesia. After the back skin is disinfected by iodine, the skin and the deep fascia of the back are cut to reach the muscular layer, and a circular wound surface with the diameter of 1.5cm is formed. After hemostasis, 3mL of female hip treatment and protection essence lotion coating film is smeared on the wound surface of the experimental group and fixed by using a bandage, and the same amount of normal saline is smeared on the blank control group.

After 3 weeks, each group of mice was sacrificed with excess ether, and the amounts of collagen type i and collagen type iii secreted from the wound surface skin tissues of the mice were counted.

The secretion amounts of type I collagen and type III collagen were determined by reference to "the influence of gelatin degradation products on proliferation of rat dermal fibroblasts and type I collagen secretion" (Jingwei, J.Microtraumatic surgery, 2007, 08 th.).

The statistical data are expressed as the multiples of the collagen type I and III secretion amounts of the experimental group relative to the collagen type I and III secretion amounts of the control group.

The specific test results are shown in table 3.

TABLE 3 statistics of type I and type III collagen secretion

The chitosan belongs to linear macromolecules and can be completely absorbed by organisms, the lecithin has good skin compatibility, and the astaxanthin, the chitosan and the lecithin are compounded, so that the stability of the astaxanthin can be improved, and the astaxanthin can be absorbed and utilized by human bodies more conveniently. In the embodiment 3 of the invention, a hydrophobic group of lecithin is introduced into a chitosan hydrophilic chain under the action of ultrasonic assistance, and self-aggregation is carried out by virtue of driving forces such as hydrophobic and electrostatic effects and the like to form a chitosan/lecithin/astaxanthin nano compound with a core-shell structure of a hydrophilic shell and a hydrophobic core.

Different from embodiment 3, embodiment 4~ 6 form the nanoparticle through chitosan and DNA macromolecule through self-assembly, exist hydrophobic micro-area in the spatial structure of nanoparticle, and hydrophobic micro-area combines together with fat-soluble and astaxanthin, wraps up astaxanthin, further improves astaxanthin water-solubility and stability for its activity and biological utilization degree improve. Wherein, in the embodiments 4-6, when the salmon sperm DNA solution is 0.02mg/mL, the chitosan/astaxanthin/DNA nano compound has the most compact combination among the molecules, and the oxidation resistance and the stability are strongest.

Effect example 3

The elasticity and moisture retention performance of the hip-care essence lotion for hip therapy of women in examples 1-8 are evaluated.

The experimental method comprises the following steps: selecting healthy, normal skin and non-cosmetic allergy history test population, wherein the age is 20-45 years, and the population is half of male and female. One week is a treatment course, four treatment courses are provided, the buttocks are cleaned before each use, the cleaning time is the same for each time, the corresponding product is used after the skin is dried, each group uses the corresponding product 1 time every day, and 10g of the product is uniformly smeared on a tested area.

Measurement: the buttocks were selected as the test area and the test was performed 10 minutes after application of the product. And measuring a skin elasticity curve by using a skin elasticity tester, and evaluating the elasticity protection performance of the female hip therapy hip protection essence lotion according to the stretching amount and the resilience data of the skin.

The experimental results are as follows: the skin elasticity test is based on the principle of suction and stretching, negative pressure is generated on the surface of the skin to be tested, the skin is sucked into a special test probe, the skin suction depth is measured through a non-contact optical detection system, the measurement result is generally expressed by an index, the unit is 1, and the larger the value is, the stronger the skin elasticity is.

The specific test results are shown in table 4.

TABLE 4 ballistic performance test value table

Group of	0 week	1 week	2 weeks	3 weeks	4 weeks
						Example 1	0.491	0.530	0.617	0.647	0.675
Example 2	0.489	0.533	0.625	0.651	0.684
						Example 3	0.494	0.558	0.658	0.691	0.726
Example 4	0.490	0.540	0.630	0.669	0.691
						Example 5	0.497	0.544	0.637	0.672	0.698
Example 6	0.496	0.565	0.671	0.695	0.737
						Example 7	0.487	0.560	0.665	0.690	0.733
Example 8	0.501	0.572	0.685	0.721	0.755

Note: after comparison, statistical analysis is carried out, and the result shows that P is less than 0.05, and the difference has statistical significance.

(II) experimental method: selecting healthy skin, and people with no cosmetic allergy history, wherein the age is 20-45 years, and the age is half of that of male and female. Room temperature 25 +/-1 ℃; humidity 50% + -5%. During the test, the buttocks are cleaned, the cleaning time of each person is the same, after the skin is dried, the skin with the buttocks size of 4cm multiplied by 4cm is selected as a test area, and 0.8g of the skin is uniformly smeared on the buttocks.

In the test area of the buttocks (4 cm. times.4 cm), the test is carried out 10 minutes after the product is to be used.

(1) The moisture content of the hip skin was measured using a digital skin moisture detector. After 1 hour, 2 hours, 4 hours and 6 hours after using the corresponding product, the water content of the skin of the face and the hip is measured, and the average value is obtained by measuring the water content three times.

(2) The amount of water loss in the hip skin was measured by a percutaneous water loss measuring instrument. After 1 hour, 2 hours, 4 hours and 6 hours after the corresponding product was used, the water dispersion loss of the hip skin was measured and the average value was taken for three times.

The experimental results are as follows: the moisture-keeping efficacy of the hip-care essence lotion for female hip therapy is evaluated by measuring the change of the water content of the stratum corneum through testing the change of the capacitance of the stratum corneum of the skin before and after the product is used. The method quantifies the moisture content of the skin cutin, can sensitively reflect the change of the moisture content of the skin, has good reproducibility, and is one of the common methods for evaluating the efficacy of the current moisturizing cosmetics.

The specific test results are shown in table 5.

Table 5 moisturizing performance test values table units: is based on

Group of	0 hour	1 hour	2 hours	4 hours	6 hours
						Example 1	30.52	62.41	60.22	57.46	56.39
Example 2	30.56	62.52	60.37	57.54	56.48
						Example 3	30.49	62.79	60.52	57.85	56.87
Example 4	30.61	63.63	60.44	57.61	56.51
						Example 5	30.47	62.65	60.49	57.74	56.69
Example 6	30.72	62.96	60.74	58.06	57.17
						Example 7	30.64	62.93	60.63	57.99	56.98
Example 8	30.57	63.09	60.82	58.18	57.29

Hyaluronic acid is an important scaffold component in the connective tissue of the skin, having the ability to bind water and good biocompatibility. However, hyaluronic acid is easily degraded, and it is understood from test examples 2 and 3 that the present invention reduces the degradation rate of hyaluronic acid, promotes the proliferation of fibroblasts and the generation of collagen, and promotes skin scar repair and wound repair by using agarose-modified hyaluronic acid. Therefore, the female hip-therapy hip-care essence disclosed by the invention can deeply penetrate into the epidermis or even the dermis of the skin to play a role in maintaining and enhancing the water absorption capacity and barrier function of the stratum corneum, and the water content of the stratum corneum is maintained, so that the skin is prevented from being dried.

Although the present description is described in terms of embodiments, not every embodiment includes only a single embodiment, and such descriptions are merely for clarity and should be taken as a whole by those skilled in the art, and the embodiments in each embodiment may be appropriately combined to form other embodiments as will be appreciated by those skilled in the art.

Claims

1. The female hip treatment and protection essence is characterized by being prepared from the following raw materials in parts by weight: 50-80 parts of water, 2-7 parts of astaxanthin nano-composite, 2-4 parts of corn sprout extract, 0.01-0.05 part of ferulic acid, 1-10 parts of glycerol, 0.5-2.5 parts of honey, 1-3 parts of ostrich oil, 0.2-0.6 part of carbomer, 0.2-0.8 part of sargassum fusiforme polysaccharide, 2-3 parts of glucoside and 0.003-0.006 part of agarose graft modified hyaluronic acid;

the astaxanthin nano-composite is a chitosan/astaxanthin/DNA nano-composite and is prepared by the following method: dissolving 500-1000 mg of chitosan into 40-80 mL of deionized water, and stirring for 2-3 hours to obtain a chitosan hydration solution; adding 40-80 mL of hydrochloric acid solution with the pH of 2 into the chitosan hydration solution, stirring for 2-3 hours, and adjusting the pH to 7 by using sodium hydroxide aqueous solution with the pH of 13; finally, deionized water is added to obtain chitosan mother liquor with the chitosan concentration of 0.02-0.04 mg/mL as solution A; dissolving 100-200 mg of astaxanthin into 150-300 mL of absolute ethyl alcohol, stirring for 24-36 hours in a dark environment, centrifuging for 5-10 minutes, and taking supernatant to obtain an astaxanthin ethanol solution as a solution B; dissolving salmon sperm DNA into deionized water, and preparing to obtain salmon sperm DNA solution with the concentration of 0.02-0.32 mg/mL as solution C; and (3) uniformly mixing the solution A and the solution B under the condition of keeping out of the sun, and adding the solution C, wherein the volume ratio of the solution A to the solution B to the solution C is 2: (0.5-3): 1, fully and uniformly mixing, and carrying out vacuum freeze drying to obtain the chitosan/astaxanthin/DNA nano compound;

the concentration of the salmon sperm DNA solution is 0.02-0.04 mg/mL;

the glucoside is polyethylene glycol starch glucoside and polyethylene glycol glucoside in a mass ratio of 1: 1, in a mixture of the components.

2. The female hip-treatment hip-care essence according to claim 1, wherein the deacetylation degree of chitosan is 90-95%.

3. The female hip therapy hip-care essence lotion according to claim 1, wherein the honey is one or more of acacia honey, all-flower honey, honey of winter honey, honey of astragalus sinicus, honey of lychee, linden honey, honey of sophora flower, honey of osmanthus flower, honey of coptis chinensis, honey of gallnut, honey of red sage root, honey of astragalus mongholicus, honey of gallnut, honey of motherwort, and honey of Chinese wolfberry.