CN109169499A - A kind of poultry cultivation method for reducing antibiotic medicine and using - Google Patents
A kind of poultry cultivation method for reducing antibiotic medicine and using Download PDFInfo
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- CN109169499A CN109169499A CN201810922302.2A CN201810922302A CN109169499A CN 109169499 A CN109169499 A CN 109169499A CN 201810922302 A CN201810922302 A CN 201810922302A CN 109169499 A CN109169499 A CN 109169499A
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Classifications
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- A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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Abstract
The invention discloses a kind of poultry cultivation methods that reduction antibiotic medicine uses, by feeding probiotics in the young bird phase, immunopotentiator is added in feed, the autoimmunity of a variety of methods enhancing cultured birds such as Immune-enhancing effect substance and atomization Chinese herbal medicine extract is added in drinking-water, and institute's application method is free of any poisonous and harmful substance, also the risk of Metabolic residue is not present, simultaneously, the characteristics of being developed according to bird reasonable arrangement difference immunologic facilitation means use the time, it is all linked with one another between means of different, resistance of the comprehensive raising birds to epidemic disease, it effectively reduces even without using antibiotic, keep birds product safer, improve culture efficiency.
Description
Technical field
The present invention relates to water pollution process field, in particular to a kind of poultry cultivation side for reducing antibiotic medicine and using
Method.
Background technique
Antibiotic be with sterilization or bacteriostasis a kind of chemical substance general name, can be used for treating humans and animals due to
Bacterium infection and caused by disease.The development of feeding antibiotic and the antibiotic that the mankind use are almost synchronous, with animal-breeding
Scale constantly increases, and the demand of animal food is multiplied, and the use of feeding antibiotic also quicklys increase therewith.
Chinese aquaculture is the maximum field of antibiotic usage amount, more than the half of domestic antibiotic total quantity consumed.Due to
China's scale in recent years, intensive aquaculture are grown rapidly, and feeding antibiotic dosage significantly increases antibiotic in aquaculture
Use be broadly divided into two large divisions.A part is that Feed Enterprise is added during producing full price, concentration, premix, main
It is used to prevent epidemic disease and promotes growth.Another part be raiser use in the breeding process, using spice, drinking-water, injection,
Gavage and environment sprinkling etc. various ways, use purpose be mostly prevent and treat livestock and poultry pestilence.
Common feeding antibiotic class drug mainly includes Tetracyclines, phthalein amine, promise ketone in p mono- on the market at present
Antibiotic etc..Tetracycline medication can prevent and treat a variety of diseases of poultry, such as typhoid fever, dysentery characterized by white mucous stool, cholera, infectiousness synovitis, infect
Property rhinitis, chronic respiratory tract disease, streptococcosis, staphylococosis, listerellosis, spirochetosis and globidiosis etc..In addition, also
With mitigation stress reaction, increases egg production and improve hatching rate equivalent force.Penicillin in interior phthalein amine antibiotic is then used for
The treatment of chicken staphylococcus aureus, colibacillosis；Head embraces bacteriums and is then chiefly used in chicken staphylococcus aureus, Salmonella bar
The prevention and treatment of bacterium, colibacillosis；Promise ketone antibiotic can treat chronic respiratory tract disease, colibacillosis, white diarrhea, golden yellow
Color staphylococosis, streptococcosis.
Although at present country in the use of veterinary antibiotic strict control, in actual production, some farms and
Antibiotic off-drug period regulation is ignored by decentralized household, to cause antibiotic residue in birds product exceeded, directly endangers eater's body
Body health.In daily animal food, if long-term, high-dose uses certain antibiotic, certain pathogenic bacteria can be made to generate anti-medicine
Property or drug resistance, when treating resulting human body diseases, the therapeutic effect of antibiotic of the same race will have a greatly reduced quality, need plus
Big drug dose, and the antibiotic for needing spectrum long-acting could work.The abuse of feeding antibiotic also leads to infecting both domestic animals and human disease
Disease increases.The animal food of intake low dosage antibiotic residue will cause antibiotic and accumulate in human body for a long time, cause each
Histoorgan lesion.It can additionally cause some special populations that hypersensitivity occurs.
Therefore, it is extremely urgent to develop a kind of method that reduction even carries out poultry cultivation without using antibiotic medicine, passes through
The immunity of birds itself is improved to inhibit the generation of epidemic disease, the safety of birds product not only can be improved, can also reduce fowl
Class aquaculture cost improves culture efficiency.
Summary of the invention
The technical problems to be solved by the invention: existing in the prior art a variety of scarce for purifying eutrophication water body
Point, the present invention provide a kind of poultry cultivation method that uses of reduction antibiotic medicine and efficiently solve these problems and disadvantage.
In order to solve the above technical problems, the present invention provides technical solution below:
A kind of poultry cultivation method for reducing antibiotic medicine and using, specifically includes the following steps:
(1) 1 age in days of birds young bird continuously gavages probiotic microcapsule respectively, and 1 times/day, totally 7 days, each every probiotics
Number is 4 × 108CFU, stomach-filling volume are 0.1mL/10g；
(2) when 7 age in days of birds young bird, immunopotentiator is added in daily ration, adding proportion is 1~10wt% of daily ration,
Addition is primary every three days, feeding to 60~90 ages in days；
(3) when 15 age in days of birds young bird, polyunsaturated fatty acid is added in daily ration and carotenoid, adding proportion are
0.1~0.5wt% of daily ration, daily to add, continuous feeding to 60~90 ages in days；
(4) after 30 age in days of birds young bird, astragalus polyose and mushroom extract, astragalus polyose adding proportion are added in drinking-water
For 1~5wt% of drinking-water, mushroom extract adding proportion is 0.1~0.5wt% of drinking-water, and addition is primary every three days, feeding
To 60~90 ages in days；
(5) after 45 age in days of birds young bird, in spraying daily primary in breeding room, spraying middle addition Chinese herbal medicine extract, mist
Dripping size is 50~150 μm, and spray time is 15~30min；
(6) stocking density of birds is 5~20/m2, at 22~30 DEG C, other method for breeding are for raising temperature control
Conventional birds method for breeding.
Preferably, the probiotic microcapsule the preparation method comprises the following steps:
(1) by the MRS fluid nutrient medium containing Bacillus acidi lactici LA-5, bacillus licheniformis saved in -80 DEG C of refrigerators point
It is not activated through MRS solid plate culture medium, picking single bacterium colony is inoculated in MRS fluid nutrient medium under gnotobasis, 37 DEG C of trainings
It supports for 24 hours, it is continuous to transfer for 3 generations, Bacillus acidi lactici LA-5 and bacillus licheniformis is collected by centrifugation, washs bacterium with sterile saline respectively
Body three times, and is resuspended and counts up to 1.6 × 1010/mL；
(2) by the bacterium solution of Bacillus acidi lactici LA-5 and bacillus licheniformis, 1:1 is mixed by volume；
(3) mixed bacteria liquid is mixed with 2% sodium alginate by 1:1 volume ratio, instills pre-cooling dropwise by lml syringe
0.2mol/L CaCl2In solution, solidify 4h, physiology salt is washed 3 times, and 4 DEG C of refrigerators save backup.
Preferably, the birds are one of chicken, duck, goose.
Preferably, the polyunsaturated fatty acid is the mixture of ω -3 and omega 6 polyunsaturated fatty acid, mass ratio ω -
3: ω -6=1:1~1:4, the omega-3 polyunsaturated fatty acids are one of linseed oil or fish oil, and the ω -6 is mostly not
Saturated fatty acid is corn oil；The carotenoid is in alpha-carotene, bata-carotene, gamma carotene and lycopene
It is at least one.
Preferably, the Chinese herbal medicine extract be Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum water extract, by Radix Isatidis,
The dried object of dandelion, Herba Kalimeridis and chrysanthemum is mixed by the weight ratio of 1:2:2:1, and mixture is added by the weight ratio of 1:100
Clear water impregnates 12h, is cooled to room temperature after boiling, is filtered with double gauze, and filtrate is for spraying.
Preferably, the mushroom extract is one of mushroom, Hericium erinaceus or cordyceps sinensis flower extract, preparation method
Are as follows:
(1) mushroom, Hericium erinaceus or cordyceps flower dry product are pulverized, clear water is added by weight 1:500, impregnates for 24 hours；
(2) slight boiling condition 3h, three layers of filtered through gauze are kept after boiling above-mentioned soak；
(3) filtrate is concentrated into the 1/20 of stoste volume by vacuum distillation at 40 DEG C；
It (4) is mushroom extract after concentrate is cooled to room temperature, aseptic subpackaged rear 4 DEG C of preservations.
Preferably, the component of the immunopotentiator is by weight are as follows: 1~3 part of vitamin E, dehydroretinol~5 part,
2~6 parts of vitamin C, 1~10 part of chitosan, 5~15 parts of cocounut oil, 0.1~0.5 part of grape seed extract, 1~5 part of zinc oxide,
0.5~2.5 part of capsaicine, 10~20 parts of medical stone, 5~25 parts of polyfructosan.
It is that the present invention obtains the utility model has the advantages that the present invention is by feeding probiotics in the young bird phase, adding immunologic facilitation in feed
Itself exempting from for a variety of methods enhancing cultured birds such as Immune-enhancing effect substance and atomization Chinese herbal medicine extract is added in agent in drinking-water
Epidemic disease power, and institute's application method is free of any poisonous and harmful substance, and the risk of Metabolic residue is also not present, meanwhile, it is raw according to birds
Long the characteristics of developing reasonable arrangement difference immunologic facilitation means use time, all linked with one another, comprehensive raising between means of different
Birds effectively reduce even without using antibiotic to the resistance of epidemic disease, keep birds product safer, improve culture efficiency.
Specific embodiment
Specific embodiments of the present invention will be described in further detail below, to help those skilled in the art couple
Inventive concept of the invention, technical solution have more complete, accurate and deep understanding.
Embodiment 1:
The blue Cold boiled chicken in sea is cultivated with the following method:
(1) probiotic microcapsule is prepared, the specific method is as follows:
(A) by the MRS fluid nutrient medium containing Bacillus acidi lactici LA-5, bacillus licheniformis saved in -80 DEG C of refrigerators point
It is not activated through MRS solid plate culture medium, picking single bacterium colony is inoculated in MRS fluid nutrient medium under gnotobasis, 37 DEG C of trainings
It supports for 24 hours, it is continuous to transfer for 3 generations, Bacillus acidi lactici LA-5 and bacillus licheniformis is collected by centrifugation, washs bacterium with sterile saline respectively
Body three times, and is resuspended and counts up to 1.6 × 1010/mL；
(B) by the bacterium solution of Bacillus acidi lactici LA-5 and bacillus licheniformis, 1:1 is mixed by volume；
(C) mixed bacteria liquid is mixed with 2% sodium alginate by 1:1 volume ratio, instills pre-cooling dropwise by lml syringe
0.2mol/L CaCl2In solution, solidify 4h, physiology salt is washed 3 times, and 4 DEG C of refrigerators save backup.
(2) 1 age in days of chick continuously gavages probiotic microcapsule respectively, and 1 times/day, totally 7 days, every prebiotic bacterium number was 4 every time
×108CFU, stomach-filling volume are 0.1mL/10g；
(3) each component accurately the preparation of immunopotentiator: is weighed by following parts by weight: 1 part of vitamin E, vitamin A 2
Part, 2 parts of vitamin C, 1 part of chitosan, 5 parts of cocounut oil, 0.1 part of grape seed extract, 1 part of zinc oxide, 0.5 part of capsaicine, wheat meal
10 parts of stone, 5 parts of polyfructosan；Said components are spare after evenly mixing；
(4) when 7 age in days of chick, immunopotentiator is added in daily ration, adding proportion is the 6wt% of daily ration, is added every three days
Add primary, feeding to 60 ages in days；Polyfructosan, cocounut oil and chitosan in immunopotentiator can effectively improve probiotics in chick
Activity in enteron aisle simultaneously promotes the proliferation of probiotics and colonizes, and capsaicine then can inhibit the breeding of pathogenic bacteria in enteron aisle, vitamin
A, E, C and grape seed extract can be anti-oxidant, and the immunity of nonspecific enhancing chicken body, medical stone and zinc oxide can supplement micro-
Secondary element.
(5) when 15 age in days of chick, polyunsaturated fatty acid is added in daily ration and carotenoid, adding proportion are daily ration
0.3wt%, daily to add, continuous feeding to 60 ages in days；Polyunsaturated fatty acid is the mixture of linseed oil and corn oil,
The mixing of 1:1 in mass ratio.Carotenoid is mixed using lycopene and alpha-carotene 2:1 in mass ratio.
(6) it prepares mushroom extract: mushroom dry product is pulverized, clear water is added by weight 1:500, impregnates for 24 hours；It will
Above-mentioned soak keeps slight boiling condition 3h, three layers of filtered through gauze after boiling；Filtrate is concentrated into stoste at 40 DEG C, by vacuum distillation
The 1/20 of volume；Concentrate is mushroom extract after being cooled to room temperature, aseptic subpackaged rear 4 DEG C of preservations.
(7) after 30 age in days of chick, astragalus polyose and mushroom extract are added in drinking-water, astragalus polyose adding proportion is drink
The 3wt% of water, mushroom extract adding proportion are the 0.3wt% of drinking-water, and addition is primary every three days, feeding to 60 ages in days；
(8) water extract is prepared using Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum: by Radix Isatidis, dandelion, Herba Kalimeridis and
The dried object of chrysanthemum is mixed by the weight ratio of 1:2:2:1, and clear water is added by the weight ratio of 1:100 in mixture, 12h is impregnated, boils
It is cooled to room temperature after boiling, is filtered with double gauze, filtrate is for spraying.
(9) after 45 age in days of chick, in spraying daily primary in breeding room, spraying middle addition Chinese herbal medicine extract, droplet is big
Small is 100 μm, spray time 15min；Chinese herbal medicine effective ingredients in droplet can be absorbed by the respiratory mucosa of chicken, be played
Invigorating the spleen to clear away damp pathogen, it is clearing heat and detoxicating, inhibit the effect of harmful bacteria proliferation, effectively improves the immunity of chicken.
(10) stocking density of the blue Cold boiled chicken in sea is 20/m2, at 22~25 DEG C, other method for breeding are equal for raising temperature control
To be raised by the conventinal breeding method in " chicken and duck goose raises new technology (second edition) ".
Embodiment 2:
Jianchang duck is cultivated with the following method:
(1) probiotic microcapsule is prepared, specific the preparation method is the same as that of Example 1；
(2) 1 age in days of duckling continuously gavages probiotic microcapsule respectively, and 1 times/day, totally 7 days, every prebiotic bacterium number was 4 every time
×108CFU, stomach-filling volume are 0.1mL/10g；
(3) each component accurately the preparation of immunopotentiator: is weighed by following parts by weight: 2 parts of vitamin E, vitamin A
3.5 parts, 4 parts of vitamin C, 6 parts of chitosan, 10 parts of cocounut oil, 0.3 part of grape seed extract, 3 parts of zinc oxide, 1.5 parts of capsaicine,
15 parts of medical stone, 15 parts of polyfructosan；Said components are spare after evenly mixing；
(4) when 7 age in days of duckling, immunopotentiator is added in daily ration, adding proportion is the 1wt% of daily ration, is added every three days
Add primary, feeding to 75 ages in days；Polyfructosan, cocounut oil and chitosan in immunopotentiator can effectively improve probiotics in duckling
Activity in enteron aisle simultaneously promotes the proliferation of probiotics and colonizes, and capsaicine then can inhibit the breeding of pathogenic bacteria in enteron aisle, vitamin
A, E, C and grape seed extract can be anti-oxidant, and the immunity of nonspecific enhancing duck body, medical stone and zinc oxide can supplement micro-
Secondary element.
(5) when 15 age in days of duckling, polyunsaturated fatty acid is added in daily ration and carotenoid, adding proportion are daily ration
0.1wt%, daily to add, continuous feeding to 75 ages in days；Polyunsaturated fatty acid is the mixture of fish oil and corn oil, by matter
Amount is mixed than 1:2.5.Carotenoid is mixed using beta carotene and alpha-carotene 1:1 in mass ratio.
(6) it prepares mushroom extract: Hericium erinaceus dry product is pulverized, clear water is added by weight 1:500, impregnates for 24 hours；
Slight boiling condition 3h, three layers of filtered through gauze are kept after above-mentioned soak is boiled；Filtrate is concentrated into original at 40 DEG C, by vacuum distillation
The 1/20 of liquid product；Concentrate is mushroom extract after being cooled to room temperature, aseptic subpackaged rear 4 DEG C of preservations.
(7) after 30 age in days of duckling, astragalus polyose and mushroom extract are added in drinking-water, astragalus polyose adding proportion is drink
The 1wt% of water, mushroom extract adding proportion are the 0.1wt% of drinking-water, and addition is primary every three days, feeding to 75 ages in days；
(8) water extract is prepared using Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum: by Radix Isatidis, dandelion, Herba Kalimeridis and
The dried object of chrysanthemum is mixed by the weight ratio of 1:1:1:1, and clear water is added by the weight ratio of 1:100 in mixture, 12h is impregnated, boils
It is cooled to room temperature after boiling, is filtered with double gauze, filtrate is for spraying.
(9) after 45 age in days of duckling, in spraying daily primary in breeding room, spraying middle addition Chinese herbal medicine extract, droplet is big
Small is 50 μm, spray time 25min；Chinese herbal medicine effective ingredients in droplet can be absorbed by the respiratory mucosa of duck, be played
Invigorating the spleen to clear away damp pathogen, it is clearing heat and detoxicating, inhibit the effect of harmful bacteria proliferation, effectively improves the immunity of duck.
(10) stocking density of Jianchang duck is 13/m2, at 24~28 DEG C, other method for breeding are for raising temperature control
By the conventinal breeding method raising in " duck duck goose raises new technology (second edition) ".
Embodiment 3:
Lion-headed goose is cultivated with the following method:
(1) probiotic microcapsule is prepared, specific the preparation method is the same as that of Example 1；
(2) young 1 age in days of goose continuously gavages probiotic microcapsule respectively, and 1 times/day, totally 7 days, every prebiotic bacterium number was 4 every time
×108CFU, stomach-filling volume are 0.1mL/10g；
(3) each component accurately the preparation of immunopotentiator: is weighed by following parts by weight: 3 parts of vitamin E, vitamin A 5
Part, 6 parts of vitamin C, 10 parts of chitosan, 15 parts of cocounut oil, 0.5 part of grape seed extract, 5 parts of zinc oxide, 2.5 parts of capsaicine, wheat
20 parts of meal stone, 25 parts of polyfructosan；Said components are spare after evenly mixing；
(4) when young 7 age in days of goose, immunopotentiator is added in daily ration, adding proportion is the 10wt% of daily ration, is added every three days
Add primary, feeding to 90 ages in days；Polyfructosan, cocounut oil and chitosan in immunopotentiator can effectively improve probiotics in young goose
Activity in enteron aisle simultaneously promotes the proliferation of probiotics and colonizes, and capsaicine then can inhibit the breeding of pathogenic bacteria in enteron aisle, vitamin
A, E, C and grape seed extract can be anti-oxidant, and the immunity of nonspecific enhancing goose body, medical stone and zinc oxide can supplement micro-
Secondary element.
(5) when young 15 age in days of goose, polyunsaturated fatty acid is added in daily ration and carotenoid, adding proportion are daily ration
0.5wt%, daily to add, continuous feeding to 90 ages in days；Polyunsaturated fatty acid is the mixture of linseed oil and corn oil,
The mixing of 1:4 in mass ratio.Carotenoid is mixed using lycopene and gamma carotene 2:3 in mass ratio.
(6) it prepares mushroom extract: cordyceps flower dry product is pulverized, clear water is added by weight 1:500, impregnates for 24 hours；
Slight boiling condition 4h, three layers of filtered through gauze are kept after above-mentioned soak is boiled；Filtrate is concentrated into original at 50 DEG C, by vacuum distillation
The 1/20 of liquid product；Concentrate is mushroom extract after being cooled to room temperature, aseptic subpackaged rear 4 DEG C of preservations.
(7) after 30 age in days of young goose, astragalus polyose and mushroom extract are added in drinking-water, astragalus polyose adding proportion is drink
The 5wt% of water, mushroom extract adding proportion are the 0.5wt% of drinking-water, and addition is primary every three days, feeding to 90 ages in days；
(8) water extract is prepared using Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum: by Radix Isatidis, dandelion, Herba Kalimeridis and
The dried object of chrysanthemum is mixed by the weight ratio of 1:3:4:2, and clear water is added by the weight ratio of 1:100 in mixture, 12h is impregnated, boils
It is cooled to room temperature after boiling, is filtered with double gauze, filtrate is for spraying.
(9) after young bird 45 age in days of goose, in spraying daily primary in breeding room, spraying middle addition Chinese herbal medicine extract, droplet is big
Small is 150 μm, spray time 30min；Chinese herbal medicine effective ingredients in droplet can be absorbed by the respiratory mucosa of goose, be played
Invigorating the spleen to clear away damp pathogen, it is clearing heat and detoxicating, inhibit the effect of harmful bacteria proliferation, effectively improves the immunity of goose.
(10) stocking density of lion-headed goose is 5/m2, at 25~30 DEG C, other method for breeding are for raising temperature control
By the conventinal breeding method raising in " goose duck goose raises new technology (second edition) ".
The detection present invention of influence for to(for) cultured birds immunity, is tested as follows:
(1) the blue Cold boiled chicken in sea, 75 days Jianchang ducks and each random pumping of 90 days lion-headed gooses for 60 days being cultivated in embodiment 1-3
Take 10, half male and half female, and control group is set, control group takes the conventional method of identical age in days to raise sea blue Cold boiled chicken, Jianchang respectively
Duck and lion-headed goose each 10, half male and half female, Culling heart blood put to death, quickly take immune organ (thymus gland, spleen, the bursa of farbricius), set
In 10% neutral formalin sucrose PBS buffer solution of 4 DEG C of refrigerators pre-cooling, for T in immune organANAE+Cell and thick liquid cell
Quantity.
(2) immune organ and tissue T lymphocyte quantity detection uses tissue paraffin section de and acid-α-naphthalene acetate esterase enzyme
(acid nons-pecific 1-naphthyl acetate esterase, ANAE) decoration method；Immune organ and tissue thick liquid cell
The detection of quantity uses DNA/RNA decoration method.
As a result as follows:
T in 1 thymus gland of tableANAE+Cell average amount (unit: × 103A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 32.8 | _ | _ |
| Embodiment 2 | _ | 33.9 | _ |
| Embodiment 3 | _ | _ | 45.2 |
| Control group | 26.8 | 29.4 | 38.4 |
T in 2 spleen of tableANAE+Cell average amount (unit: × 103A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 39.8 | _ | _ |
| Embodiment 2 | _ | 42.9 | _ |
| Embodiment 3 | _ | _ | 48.4 |
| Control group | 31.9 | 33.4 | 40.1 |
T in 3 French capsule of tableANAE+Cell average amount (unit: × 103A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 21.1 | _ | _ |
| Embodiment 2 | _ | 26.8 | _ |
| Embodiment 3 | _ | _ | 25.7 |
| Control group | 14.3 | 18.4 | 21.3 |
4 thymus gland mesoplasmatocyte par (unit: × 10 of table3A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 15.2 | _ | _ |
| Embodiment 2 | _ | 14.9 | _ |
| Embodiment 3 | _ | _ | 23.4 |
| Control group | 9.8 | 10.4 | 12.1 |
5 spleen mesoplasmatocyte par (unit: × 10 of table3A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 28.3 | _ | _ |
| Embodiment 2 | _ | 29.4 | _ |
| Embodiment 3 | _ | _ | 31.1 |
| Control group | 18.8 | 20.9 | 25.8 |
6 French capsule mesoplasmatocyte par (unit: × 10 of table3A/mm2)
| Group | Extra large orchid Cold boiled chicken | Jianchang duck | Lion-headed goose |
| Embodiment 1 | 14.4 | _ | _ |
| Embodiment 2 | _ | 16.9 | _ |
| Embodiment 3 | _ | _ | 20.8 |
| Control group | 8.8 | 9.4 | 10.9 |
By the result of table 1-6 as it can be seen that the present invention can effectively improve the number of T cell and B cell in the immune organ of chicken and duck goose
Amount, effectively increases the inherent immunity and adaptive immunity power of cultured birds.
In conclusion the present invention is by adding immunopotentiator in young bird phase feeding probiotics, feed, adding in drinking-water
Add the autoimmunity of a variety of methods enhancing cultured birds such as Immune-enhancing effect substance and atomization Chinese herbal medicine extract, and is used
Method is free of any poisonous and harmful substance, and the risk of Metabolic residue is also not present, meanwhile, the characteristics of being developed according to bird conjunction
Reason arranges the time that uses of different immunologic facilitation means, and all linked with one another between means of different, comprehensive raising birds support epidemic disease
Drag effectively reduces even without using antibiotic, keeps birds product safer, improves culture efficiency.
The above examples only illustrate the technical idea of the present invention, and this does not limit the scope of protection of the present invention, all
According to the technical idea provided by the invention, any changes made on the basis of the technical scheme each falls within the scope of the present invention
Within；The technology that the present invention is not directed to can be realized by the prior art.
Claims (7)
1. a kind of poultry cultivation method for reducing antibiotic medicine and using, which is characterized in that specifically include the following steps:
(1) 1 age in days of birds young bird continuously gavages probiotic microcapsule respectively, and 1 times/day, totally 7 days, every prebiotic bacterium number was 4 every time
×108CFU, stomach-filling volume are 0.1mL/10g；
(2) when 7 age in days of birds young bird, immunopotentiator is added in daily ration, adding proportion is 1~10wt% of daily ration, every three
Its addition is primary, feeding to 60~90 ages in days；
(3) when 15 age in days of birds young bird, polyunsaturated fatty acid is added in daily ration and carotenoid, adding proportion are daily ration
0.1~0.5wt%, daily to add, continuous feeding to 60~90 ages in days；
(4) after 30 age in days of birds young bird, astragalus polyose and mushroom extract are added in drinking-water, astragalus polyose adding proportion is drink
1~5wt% of water, mushroom extract adding proportion are 0.1~0.5wt% of drinking-water, and addition is primary every three days, feeding to 60
~90 ages in days；
(5) after 45 age in days of birds young bird, in spraying daily primary in breeding room, spraying middle addition Chinese herbal medicine extract, droplet is big
Small is 50~150 μm, and spray time is 15~30min；
(6) stocking density of birds is 5~20/m2, at 22~30 DEG C, other method for breeding are routine for raising temperature control
Birds method for breeding.
2. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, which is characterized in that institute
State probiotic microcapsule the preparation method comprises the following steps:
(1) the MRS fluid nutrient medium containing Bacillus acidi lactici LA-5, bacillus licheniformis saved in -80 DEG C of refrigerators is passed through respectively
MRS solid plate culture medium activates, and picking single bacterium colony is inoculated in MRS fluid nutrient medium under gnotobasis, 37 DEG C of cultures
For 24 hours, it continuously transferred for 3 generations, Bacillus acidi lactici LA-5 and bacillus licheniformis is collected by centrifugation, use sterile saline washing thalline respectively
Three times, and resuspension counts up to 1.6 × 1010/mL；
(2) by the bacterium solution of Bacillus acidi lactici LA-5 and bacillus licheniformis, 1:1 is mixed by volume；
(3) mixed bacteria liquid is mixed with 2% sodium alginate by 1:1 volume ratio, instills pre-cooling dropwise by lml syringe
0.2mol/L CaCl2In solution, solidify 4h, physiology salt is washed 3 times, and 4 DEG C of refrigerators save backup.
3. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, it is characterised in that: institute
Stating birds is one of chicken, duck, goose.
4. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, it is characterised in that: institute
State polyunsaturated fatty acid be ω -3 and omega 6 polyunsaturated fatty acid mixture, ω -3: ω -6=1:1 of mass ratio~1:4,
The omega-3 polyunsaturated fatty acids are one of linseed oil or fish oil, and the omega 6 polyunsaturated fatty acid is corn
Oil；The carotenoid is at least one of alpha-carotene, bata-carotene, gamma carotene and lycopene.
5. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, it is characterised in that: institute
State Chinese herbal medicine extract be Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum water extract, by Radix Isatidis, dandelion, Herba Kalimeridis and chrysanthemum
Colored dried object is mixed by the weight ratio of 1:2:2:1, and clear water is added by the weight ratio of 1:100 in mixture, 12h is impregnated, boils
After be cooled to room temperature, filtered with double gauze, filtrate is for spraying.
6. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, it is characterised in that: institute
Stating mushroom extract is one of mushroom, Hericium erinaceus or cordyceps sinensis flower extract, preparation method are as follows:
(1) mushroom, Hericium erinaceus or cordyceps flower dry product are pulverized, clear water is added by weight 1:500, impregnates for 24 hours；
(2) slight boiling condition 3h, three layers of filtered through gauze are kept after boiling above-mentioned soak；
(3) filtrate is concentrated into the 1/20 of stoste volume by vacuum distillation at 40 DEG C；
It (4) is mushroom extract after concentrate is cooled to room temperature, aseptic subpackaged rear 4 DEG C of preservations.
7. a kind of poultry cultivation method for reducing antibiotic medicine and using according to claim 1, it is characterised in that: institute
State the component of immunopotentiator by weight are as follows: 1~3 part of vitamin E, 2~5 parts of vitamin A, 2~6 parts of vitamin C,
1~10 part of chitosan, 5~15 parts of cocounut oil, 0.1~0.5 part of grape seed extract, 1~5 part of zinc oxide, capsaicine 0.5~2.5
Part, 10~20 parts of medical stone, 5~25 parts of polyfructosan.
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Cited By (1)
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| CN112042832A (en) * | 2020-09-28 | 2020-12-08 | 井冈山大学 | Poultry breeding microbial feed additive and preparation method and application thereof |
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Application publication date: 20190111 |