CN109134012A - A kind of technique of resource utilization human faecal mass manufacture biological organic fertilizer - Google Patents
A kind of technique of resource utilization human faecal mass manufacture biological organic fertilizer Download PDFInfo
- Publication number
- CN109134012A CN109134012A CN201811096106.0A CN201811096106A CN109134012A CN 109134012 A CN109134012 A CN 109134012A CN 201811096106 A CN201811096106 A CN 201811096106A CN 109134012 A CN109134012 A CN 109134012A
- Authority
- CN
- China
- Prior art keywords
- organic fertilizer
- resource utilization
- technique
- biological organic
- human feces
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
- C05F3/04—Fertilisers from human or animal excrements, e.g. manure from human faecal masses
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Fertilizers (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of techniques of resource utilization human feces manufacture biological organic fertilizer, efficient, green human excrement fermenting organism organic fertilizer is developed using excrement mixed microorganism solid fermentation process, wherein complex micro organism fungicide includes bacillus coagulans, bacillus subtilis and sporotrichum thermophile bacterium.The technique uses more bacterium mixed fungus fermentations, by the combination of different metabolic ability, the complicated metabolism that single strain is difficult to complete, effective conversion by cooperative fermentation between microorganism to human excrement are completed, sanitary index is up to state standards requirement, and product is decomposed and growth-promoting long pointer is obviously improved.The zymotechnique is simple and efficient, deodorizing sterilizing effect is good, and production cost is low, is easy to industrial-scale production.
Description
Technical field
It is especially a kind of compound micro- the present invention relates to a kind of technique of resource utilization human feces manufacture biological organic fertilizer
Bacteria agent solid fermentation night soil quickly prepares the production technology of biological organic fertilizer, belongs to bioengineering field.
Background technique
Currently, dry pail latrine transformation is generally carried out in China's the vast rural areas, also produced while clean rural environment
A large amount of waste is likely to generate secondary pollution as resource utilization is improper, if realizing, resource utilization is not only contributed to
The secondary pollution of rural environment is avoided, and is conducive to turn waste into wealth, realizes resource utilization, thus there is highly important meaning
Justice.Due in human excrement contain a large amount of human urine, at present in terms of innoxious use human excrement mainly by anaerobic methane ferment or
Compost fermentation as patent CN201410015979 discloses a kind of novel quick human excrement composting process, and specifically discloses harmless
Change processing, high temperature is decomposed, liquid film modification process flow.But for current technique, anaerobic methane zymotechnique requires more multiple
It is miscellaneous, it is difficult in different zones, Various Seasonal popularity application, to be such as difficult in the area that north of china in winter does not have exogenous heat source
Realize producing methane through anaerobic fermentation；The shortcomings that composting fermentation technology, needs to separate using fecaluria more, and the compost fermentation time is longer, temperature
It is higher, it not only be easy to cause secondary pollution but also increases production cost, it is difficult to popularity application.
In terms of resource utilization human excrement prepares biological organic fertilizer, patent 201710087922.4 discloses a kind of recycling
Using the method for waste production bio-organic fertilizer, this method is produced using waste and building waste using fermentation technique technique
Fertilizer efficiency to bio-organic fertilizer, the fertilizer is good, and resource utilization rate is high, and low production cost；But the patent is not directed to
Human excrement and human urine are mixed to the technical inspiration that biological organic fertilizer is prepared using solid fermentation.In terms of comprehensive, in the field at present
Comprehensive utilization night soil solid fermentation prepares biological organic fertilizer and is rarely reported.
Summary of the invention
In order to overcome the above-mentioned deficiencies of the prior art, meet the harmless treatment of dry pail latrine night soil and sustainable agriculture development
Demand to the bio-organic fertilizer with efficient growth-promoting, volume increase, improvement soil and other effects, the present invention provide a kind of recycling benefit
The technique that biological organic fertilizer is manufactured with human feces integrates modern mixed bacteria microorganism fermentation technique, develops efficient, green
Fertilizer Transformed high efficient technology system, which is simple and efficient, resource utilization rate is high, and production cost is low, is easy to industrialize
Large-scale production.
The present invention is achieved through the following technical solutions above-mentioned technical effect:
A kind of technique of resource utilization human feces manufacture biological organic fertilizer, it is characterised in that after mixing night soil with auxiliary material
Then biology is made by solid fermentation, screening, granulation, cooling dry, screening, technology of the package in inoculating complex microorganism microbial inoculum
Organic fertilizer, specific preparation process include:
(1) mixing: night soil is squeezed into mixing machine by slurries pump, adds plant waste as auxiliary material, by auxiliary material and human excrement
Urine is uniformly mixed, and material is then delivered to fermentor by auger；
(2) compound microorganism ferments: then the inoculating complex microorganism microbial inoculum into mixed material is aerated solid fermentation
3~5 days, wherein the complex micro organism fungicide includes bacillus coagulans, bacillus subtilis and sporotrichum thermophile bacterium；
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration；
(4) it pelletizes: material input granulator after screening is granulated；
(5) cooling drying: the material after granulation is inputted into cooler, the moisture content of material 15~18% after cooling drying；
(6) regrading: the material after drying is sieved, and removes powder；
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Auxiliary material in above-mentioned steps (1) is one or more of sawdust, rice bran, wheat bran, agricultural crop straw, mushroom residue
Mixture.
The standard of night soil and ratio of adjuvant is between mixed material moisture 55~65% in above-mentioned steps (1).
The inoculum concentration of above-mentioned steps (2) complex micro organism fungicide is the 0.2~0.3% of solid material mass ratio.
Bacillus coagulans, bacillus subtilis in microbial bacterial agent in above-mentioned steps (2) in complex micro organism fungicide
Amount ratio with sporotrichum thermophile bacterium is 2~3:3~5:2~4.
Further, bacillus coagulans, withered grass in the microbial bacterial agent in above-mentioned steps (2) in complex micro organism fungicide
The optimal amount ratio of bacillus and sporotrichum thermophile bacterium is 3:4.5:3.
The seed liquor of above-mentioned bacillus coagulans and bacillus subtilis is the preparation method comprises the following steps: aseptically, using LB
Culture medium is cultivated for 24 hours under the conditions of 28~32 DEG C, and liquid condensation bacillus, bacillus subtilis, seed liquor is made, it is desirable that kind
Living bacteria count >=10 of sub- liquid9cfu/mL。
The seed liquor of above-mentioned sporotrichum thermophile bacterium the preparation method comprises the following steps: aseptically, using potato culture medium 35
It is cultivated under the conditions of~40 DEG C for 24 hours, sporotrichum thermophile bacterium seed liquor is made, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
It is carried out in Sealed Solid fermentor in above-mentioned solid fermentation process, fermentation ventilation quantity maintains heap body oxygen content to exist
12~16%；Deodorization device is equipped at the top of fermentor.
The present invention provides a kind of technique of resource utilization human feces manufacture biological organic fertilizer, gained fermenting organism has
Machine fertilizer stink all disappears, and quality is soft, not agglomerating, rich in the nutritional ingredients such as probiotics, water soluble organic nitrogen, product in product
The middle content of organic matter >=60%, content of microorganisms >=200,000,000/g；Product quality meets the regulation of NY525-2012, and roundworm egg is dead
Rate and coli-group number index meet the requirement of NY884-2012.It is significant that results from pot experiment test shows that the application product has
Growth promotion, improves the effects of soil environment at volume increase, is a kind of excellent novel bio-organic fertilizer.
The present invention provides a kind of technique of resource utilization human feces manufacture biological organic fertilizer, compared with prior art,
With following significant advantage:
(1) the application direct utilization night soil is changed after traditional resourceization is first separated by solid-liquid separation using night soil and is sent out again
The state of the art that ferment utilizes or direct liquid fermentation utilizes, has broken traditional inertia thinking, has reduced resource utilization cost,
Improve resource utilization efficiency.
(2) the application directly carries out solid fermentation using complex micro organism fungicide and prepares biological organic fertilizer, makes full use of micro-
Synergistic effect between biological inoculum, elimination restriction night soil Fertilizer Transformed is big using existing stink, there are a large amount of pathogenic bacteria, a huge sum of money
Belong to the drawbacks such as exceeded, improve the fertilizer efficiency of fermenting organism organic fertilizer, to be obviously improved the fertile value of human excrement, in which:
The ability of sporotrichum thermophile bacterium assimilation starch and cellulose is strong, the structural carbohydrate of human excrement can be degraded to bacillus
The monosaccharide substance such as available monosaccharide and disaccharide, makes bacillus subtilis, bacillus coagulans etc. be able to good growth；It is withered
Careless bacillus can kill the pathogenic bacteria in human excrement during hot fermentation, and can improve prebiotic in bio-organic fertilizer
Bacterial content makes bio-organic fertilizer rich in a large amount of probiotics, can effectively adjust the balance of Soil Microorganism, decomposes in soil
The organic or inorganics ingredient such as organic matter, minerals eliminates soil hardening, desertification, salinization caused by relying on chemical fertilizer abuse throughout the year etc.,
It is effectively improved soil, improves the special efficacy of crop anti-adversity, improves crops production capacity；Can especially it promote organic in soil
The release of matter improves the physicochemical structure of salinization soil, increases soil agreegate and permeability, improves soil fertility；Condensation
Bacillus belongs to facultative anaerobic bacteria, can grow under aerobic and oxygen free condition, and the application passes through addition bacillus coagulans
Overcome the defect that microbial bacterial agent can not be grown in anaerobism subenvironment that may be present in solid-state fermentation tank, and test result table
Bright, the pH value of fermentation medium can be significantly reduced in the presence of bacillus coagulans, promotes sporotrichum thermophile bacterium and withered grass gemma
The growth reproduction of bacillus.
(3) good using solid this application provides a kind of technique of resource utilization human feces manufacture biological organic fertilizer
Aerobe fermentation technique will can effectively eliminate the pathogenic bacteria such as Shigella present in human excrement, and can be by odorous substance NH3It transforms into
Nitrate, H2S is changed into H2SO4, other substances can be converted to CO2And H2O.Sporotrichum thermophile bacterium and bacillus subtilis can divide
It secretes a variety of enzymes and helps degraded cellulose and poisonous and harmful substance, improve the rotten degree of human excrement, significantly improve the growth-promoting of product
Increase production effect.
(4) the application uses two step sieving technologies, masonry tile fragment of not going out before solid fermentation process, plastics chemical fibre etc.
Impurity, but once sieved after fermentation, to remove above-mentioned impurity, the cost of removal of impurities is reduced, improves removal of impurities effect
Fruit.
(5) present invention uses solid fermentation, and technological condition for fermentation is mild, and low energy consumption, production cost is low, and the technical program is raw
Producing cost is only to have reported the 1/2 of technical level at present, important using having to industrial economy benefit and large-scale promotion is promoted
Effect；And non-environmental-pollution, investment it is small, be easy to large-scale production.
Specific embodiment
The seed liquor of fermenting microbe in following embodiment is all made of following technique:
(1) seed liquor of bacillus coagulans and bacillus subtilis is the preparation method comprises the following steps: aseptically, using LB culture medium
It is cultivated under the conditions of 28~32 DEG C for 24 hours, liquid condensation bacillus, bacillus subtilis, seed liquor is made, it is desirable that seed liquor
Living bacteria count >=109cfu/mL。
(2) seed liquor of sporotrichum thermophile bacterium the preparation method comprises the following steps: aseptically, using potato culture medium 35~
It is cultivated under the conditions of 40 DEG C for 24 hours, sporotrichum thermophile bacterium seed liquor is made, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
Embodiment 1
A kind of technique of resource utilization human feces manufacture biological organic fertilizer, specific process step include:
(1) mixing: night soil is squeezed into mixing machine by slurries pump, auxiliary material is added, auxiliary material and night soil is uniformly mixed, control
Then material is delivered to fermentor by auger by material moisture 55~65%；Wherein the auxiliary material is rice bran；
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration 0.25%, then into
Row ventilation solid fermentation 4 days, wherein the complex micro organism fungicide includes bacillus coagulans, bacillus subtilis and thermophilic side
Spore mould；Bacillus coagulans, bacillus subtilis and sporotrichum thermophile bacterium in microbial bacterial agent in complex micro organism fungicide
Optimal amount ratio be 3:4.5:3；Ventilation quantity of fermenting maintains heap body oxygen content 12~16%；
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration；
(4) it pelletizes: material input granulator after screening is granulated；
(5) cooling drying: the material after granulation is inputted into cooler, the moisture content of material 15~18% after cooling drying；
(6) regrading: the material after drying is sieved, and removes powder；
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 2
A kind of technique of resource utilization human feces manufacture biological organic fertilizer, specific process step include:
(1) mixing: night soil is squeezed into mixing machine by slurries pump, auxiliary material is added, auxiliary material and night soil is uniformly mixed, control
Then material is delivered to fermentor by auger by material moisture 55~65%；Wherein the auxiliary material is wheat bran；
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration 0.2%, then into
Row ventilation solid fermentation 5 days, wherein the complex micro organism fungicide includes bacillus coagulans, bacillus subtilis and thermophilic side
Spore mould；Bacillus coagulans, bacillus subtilis and thermophilic side spore in microbial bacterial agent in self weight complex micro organism fungicide
The amount ratio of mould is 3:5:4；Ventilation quantity of fermenting maintains heap body oxygen content 12~16%；
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration；
(4) it pelletizes: material input granulator after screening is granulated；
(5) cooling drying: the material after granulation is inputted into cooler, the moisture content of material 15~18% after cooling drying；
(6) regrading: the material after drying is sieved, and removes powder；
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 3
A kind of technique of resource utilization human feces manufacture biological organic fertilizer, specific process step include:
(1) mixing: night soil is squeezed into mixing machine by slurries pump, auxiliary material is added, auxiliary material and night soil is uniformly mixed, control
Then material is delivered to fermentor by auger by material moisture 55~65%；Wherein the auxiliary material is agricultural crop straw；
(2) compound microorganism ferments: the inoculating complex microorganism microbial inoculum into mixed material, inoculum concentration 0.3%, then into
Row ventilation solid fermentation 3 days, wherein the complex micro organism fungicide includes bacillus coagulans, bacillus subtilis and thermophilic side
Spore mould；Wherein bacillus coagulans, bacillus subtilis and thermophilic side spore in the microbial bacterial agent in complex micro organism fungicide
The amount ratio of mould is 2:5:2；Ventilation quantity of fermenting maintains heap body oxygen content 12~16%；
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration；
(4) it pelletizes: material input granulator after screening is granulated；
(5) cooling drying: the material after granulation is inputted into cooler, the moisture content of material 15~18% after cooling drying；
(6) regrading: the material after drying is sieved, and removes powder；
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
Embodiment 4 compares influence of the different fermentations technique to bio-organic fertilizer index
1, index determining method
Content of cellulose: using acid detergent method (ADF), weighs the air-dry sample 1.00g sieved by 1mm, is added with 16
The acid detergent that the sulfuric acid of alkyl trimethyl ammonium bromide (CTAB) and 0.50mol/L are prepared, heating are condensed back after boiling
60min, then sample is depressurized with glass pot formula filter and is filtered, residue is stirred after dissipating and use hot water injection, and instills acetone promotion
Colloidal substance dissolution drains acetone after filter is net, is put into 3h in 100 DEG C of air dry ovens, and cooling weighing obtains crude fibre after calculating
Percentage composition；
Content of humic acid: weighing 20 mesh sample 0.2g, and the 1L alkalinity leaching configured with 15g sodium pyrophosphate and 7g sodium hydroxide is added
Extract 70mL, boiling water bath heat 30min, take 5mL that 0.8N potassium bichromate 5mL and the 15mL concentrated sulfuric acid, then boiling water bath is added after constant volume
It after heating 30min, instills Phen indicator 3 and drips, become brick-red for titration from yellow green with ferrous sulfate titration solution
Terminal records ferrous sulfate dosage, and replaces sample to do blank test with above-mentioned steps with quartz sand；
The measurement of germination index: fresh sample mixes (W/V) with deionized water with 1:10, is placed in mistake after horizontal shaker concussion 2h
Filter.It takes 5mL filtrate to be added to be covered in the culture dish of filter paper, 20 Lepidium apetalum seeds is placed in each culture dish, blank control is
Deionized water.Culture dish is placed in 25 DEG C of constant incubators after dark culture 3 days, measures chitting piece number and root long, each
Sample setting repeats three times.Chitting piece number=sample germination percentage (%) × sample root long × 100%/control germination percentage (%) × sample
Root long；
2, test grouping
Test is divided into embodiment 1-3 test group, (redistilled water replaces complex micro organism fungicide to blank control group, remaining technique is the same as real
Apply example 1), control 1 group (complex micro organism fungicide contains only sporotrichum thermophile bacterium, remaining zymotechnique includes the same embodiment of inoculum concentration
1) it, compares 2 groups (complex micro organism fungicide contains only bacillus subtilis, remaining zymotechnique includes inoculum concentration with embodiment 1), right
According to 3 groups (complex micro organism fungicide contains only bacillus coagulans, remaining zymotechnique includes inoculum concentration with embodiment 1)；
3, data analysis statistical
The random single factor analysis of data application SPSS11.5 software, and LSD multiple comparison graph is used, with " average value ± standard
Poor (SD) " is indicated；
4, test result
Influence of 1 different technology conditions of table to bio-organic fertilizer index
| Testing index | Crude fibre (%) | Humic acid (%) | Germination index (%) |
| Blank control group | 36.34±2.63 | 10.83±0.91 | 29.18±1.73 |
| 1 test group of embodiment | 10.05±0.87 | 33.12±2.03 | 90.31±2.05 |
| 2 test group of embodiment | 14.76±1.08 | 29.25±1.66 | 89.72±1.92 |
| 3 test group of embodiment | 13.73±1.56 | 30.77±2.18 | 87.86±2.28 |
| Compare 1 group | 27.07±1.21 | 20.16±1.81 | 40.54±1.79 |
| Compare 2 groups | 30.52±1.58 | 24.65±1.29 | 57.83±1.95 |
| Compare 3 groups | 33.95±0.97 | 14.02±1.15 | 36.39±1.78 |
Above-mentioned test result shows that the synergistic effect between composite microbial fermentation bacteria group has been obviously improved the growth promoting volume increase effect of product
Fruit, the composition for changing fermentative microflora can significantly reduce product rotten index.
The above examples are only used to illustrate the technical scheme of the present invention, rather than is limited；Although referring to aforementioned reality
It applies example to be described in detail to by invention, but for those of ordinary skill in the art, it still can be to aforementioned reality
Technical solution documented by example is applied to modify or equivalent replacement of some of the technical features；And to these modifications
Or replacement, the spirit and scope for claimed technical solution of the invention that it does not separate the essence of the corresponding technical solution.
Claims (9)
1. a kind of technique of resource utilization human feces manufacture biological organic fertilizer, it is characterised in that mix night soil with auxiliary material
After be inoculated with complex micro organism fungicide, life is then made by solid fermentation, screening, granulation, cooling dry, screening, technology of the package
Organic fertilizer, specific preparation process include:
(1) mixing: night soil is squeezed into mixing machine by slurries pump, adds plant waste as auxiliary material, by auxiliary material and human excrement
Urine is uniformly mixed, and material is then delivered to fermentor by totally-enclosed auger；
(2) compound microorganism ferments: then the inoculating complex microorganism microbial inoculum into mixed material is aerated solid fermentation
3~5 days, wherein the complex micro organism fungicide will include bacillus coagulans, bacillus subtilis and thermophilic side spore in excrement
Mould；
(3) it sieves: the material after fermentation is squeezed into screening machine, removal masonry tile fragment, plastics chemical fibre and bulky grain agglomeration and sundries；
(4) it pelletizes: material input granulator after screening is granulated；
(5) cooling drying: the material after granulation is inputted into cooler, the moisture content of material 15~18% after cooling drying；
(6) regrading: the material after drying is sieved, and removes powder；
(7) it packs: the material after regrading is packed, that is, be worth bio-organic fertilizer.
2. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
Auxiliary material in the step (1) is the mixing of one or more of sawdust, rice bran, wheat bran, agricultural crop straw, mushroom residue
Object.
3. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
The standard of night soil and ratio of adjuvant is between mixed material moisture 55~65% in the step (1).
4. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
In the step (2) complex micro organism fungicide inoculum concentration be solid material mass ratio 0.2~0.3%.
5. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
Bacillus coagulans in microbial bacterial agent in the step (2) in complex micro organism fungicide, bacillus subtilis and thermophilic
The amount ratio of side spore mould is 2~3:3~5:2~4.
6. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
Bacillus coagulans in microbial bacterial agent in the step (2) in complex micro organism fungicide, bacillus subtilis and thermophilic
The optimal amount ratio of side spore mould is 3:4.5:3.
7. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
In the seed liquor of the bacillus coagulans and bacillus subtilis the preparation method comprises the following steps: aseptically, using LB culture medium
It is cultivated under the conditions of 28~32 DEG C for 24 hours, liquid condensation bacillus, bacillus subtilis, seed liquor is made, it is desirable that seed liquor
Living bacteria count >=109cfu/mL。
8. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
In the sporotrichum thermophile bacterium seed liquor the preparation method comprises the following steps: aseptically, using potato culture medium at 35~40 DEG C
Under the conditions of cultivate for 24 hours, be made sporotrichum thermophile bacterium seed liquor, it is desirable that living bacteria count >=10 of seed liquor9cfu/mL。
9. a kind of technique of resource utilization human feces manufacture biological organic fertilizer according to claim 1, feature exist
Carried out in Sealed Solid fermentor in the solid fermentation process, fermentation ventilation quantity maintain heap body oxygen content 12~
16%；Deodorization device is equipped at the top of fermentor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811096106.0A CN109134012A (en) | 2018-09-19 | 2018-09-19 | A kind of technique of resource utilization human faecal mass manufacture biological organic fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811096106.0A CN109134012A (en) | 2018-09-19 | 2018-09-19 | A kind of technique of resource utilization human faecal mass manufacture biological organic fertilizer |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109134012A true CN109134012A (en) | 2019-01-04 |
Family
ID=64814972
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811096106.0A Pending CN109134012A (en) | 2018-09-19 | 2018-09-19 | A kind of technique of resource utilization human faecal mass manufacture biological organic fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109134012A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115960970A (en) * | 2022-11-22 | 2023-04-14 | 哈尔滨工业大学 | Method for producing hydrogen by fermenting human excrement |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140079669A1 (en) * | 2004-07-13 | 2014-03-20 | William Brower | Formulation and method for treating plants to control or suppress a plant pathogen |
| CN104261913A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process of preparing pig manure bio-organic fertilizer by microbial fermentation method |
| CN105693412A (en) * | 2016-04-13 | 2016-06-22 | 嘉兴职业技术学院 | Preparation method of goat manure organic fertilizer and processing equipment thereof |
| CN107857689A (en) * | 2017-12-28 | 2018-03-30 | 青岛嘉瑞生物技术有限公司 | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure |
-
2018
- 2018-09-19 CN CN201811096106.0A patent/CN109134012A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20140079669A1 (en) * | 2004-07-13 | 2014-03-20 | William Brower | Formulation and method for treating plants to control or suppress a plant pathogen |
| CN104261913A (en) * | 2014-09-30 | 2015-01-07 | 青岛嘉瑞生物技术有限公司 | Process of preparing pig manure bio-organic fertilizer by microbial fermentation method |
| CN105693412A (en) * | 2016-04-13 | 2016-06-22 | 嘉兴职业技术学院 | Preparation method of goat manure organic fertilizer and processing equipment thereof |
| CN107857689A (en) * | 2017-12-28 | 2018-03-30 | 青岛嘉瑞生物技术有限公司 | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115960970A (en) * | 2022-11-22 | 2023-04-14 | 哈尔滨工业大学 | Method for producing hydrogen by fermenting human excrement |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100509709C (en) | Process of twice fermenting garbage-sludge mixture to produce microbial fertilizer | |
| CN107162656A (en) | A kind of feces of livestock and poultry superhigh temperature compost method | |
| CN107857689A (en) | The technique that a kind of bioanalysis prepares bio-organic fertilizer using chicken manure | |
| CN106748341A (en) | Potato microbial bacterial manure special and preparation method thereof | |
| CN105218177B (en) | The method for preparing composite microbic bacterial fertilizer using silkworm excrement fermentation | |
| CN101463334B (en) | Fermentation liquor composition for preparing biological organic fertilizer and preparation and use thereof | |
| CN109180236A (en) | A kind of new process of aerobic fermentation processing chicken manure | |
| CN101817702A (en) | Special bio-organic fertilizer for peaches and preparation method thereof | |
| CN101863692A (en) | Method for preparing bioorganic fertilizer by using city life sludge | |
| CN105384480A (en) | Biological humic acid organic material decomposition agent having deodorization function | |
| CN101914445B (en) | Indigenous probiotic microorganism solid fungicide and preparation method and application thereof | |
| CN103992184A (en) | Bio-organic fertilizer | |
| CN111254079B (en) | Composite fermentation microbial inoculum and application thereof in preparation of citrus pulp bio-organic fertilizer | |
| CN112501072B (en) | Crop rhizosphere soil conditioner and preparation method and application thereof | |
| CN109022327A (en) | A kind of preparation method of microorganism mix bacterium agent and the application in During High-Temperature Composting | |
| CN106946630A (en) | A kind of organic fungi-manure using sludge as raw material and preparation method thereof | |
| CN109180240A (en) | A kind of method of quick aerobic compost | |
| CN104293719B (en) | Fast decomposing agent for fermentation bed aging padding, organic fertilizer and production method of organic fertilizer | |
| CN109134008A (en) | A method of organic fertilizer is prepared using microorganism decomposition pig manure | |
| CN107879774A (en) | A kind of preparation method of biological decomposing agent | |
| CN103319253B (en) | Preparation method for composite microorganism fertilizer specially used for improving soil for vegetables and composite microorganism fertilizer | |
| CN106699474A (en) | Novel bio-organic fertilizer and preparation method thereof | |
| CN107311744A (en) | A kind of method that utilization pig manure produces organic fertilizer | |
| CN105016908A (en) | Compound microbial fertilizer and preparation method thereof | |
| CN107937303A (en) | A kind of method of extreme thermophilic cooperative fermentation production composite ultra-high temperature compost bacterium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20190312 Address after: 250000 No. 19 Sangyuan Road, Jinan City, Shandong Province Applicant after: Shandong Academy of Agricultural Machinery Sciences Applicant after: SHANDONG HUIMIN AVIAN LE ANIMAL HUSBANDRY MACHINERY Co.,Ltd. Address before: 251700 No. 315 Qingzi Road, Huimin County, Binzhou City, Shandong Province Applicant before: SHANDONG HUIMIN AVIAN LE ANIMAL HUSBANDRY MACHINERY Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |
|
| RJ01 | Rejection of invention patent application after publication |