CN109123680A - 一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法 - Google Patents
一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法 Download PDFInfo
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Abstract
本发明涉及益生菌制剂技术领域,具体涉及一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法,其原料是由嗜酸乳杆菌干粉30‑50份、嗜热链球菌干粉20‑35份、罗伊氏乳杆菌干粉15‑30份、乳双歧杆菌干粉30‑40份、长双歧杆菌干粉25‑35份、鼠李糖乳杆菌干粉15‑25份、低聚果糖6‑15份、低聚木糖5‑10份和纳豆粉8‑20份混合制成。本发明采用最佳科学配比,各组分相互配合,协同作用,共同调节机体肠道正常菌群,保持微生态平衡,而且在较长时间内稳定性好,保质期长,含糖量低;本发明制备方法操作简便、运行可靠、生产效益高,适用于工业化生产。
Description
技术领域
本发明涉及益生菌制剂技术领域,具体涉及一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法。
背景技术
菌群平衡与肠道健康息息相关,肠道菌群包括有益菌和有害菌,健康人体中有益菌占优势,并与有害菌相互作用,共同维持着肠内微生态的平衡,维持人体健康。如果因各种因素影响而使肠道中的益生菌减少,有害菌大量繁殖,肠内微生态平衡被打破,就会引起腹泻等多种临床症状。临床上通常会大量使用广谱抗生素,虽然能有效杀灭有害菌,但也会同时杀灭有益菌,造成肠道菌群失调,导致疾病。
对于肠道菌群失调者,经口补充益生菌制剂是直接有效调节肠道菌群的办法。其可以直接或者间接地调整宿主肠道微生物的组成,激活宿主内源性微生物群或者免疫系统的活性来实现益生作用。
目前市面上的各种益生菌食品,制造商为求适应消费者怕酸的口味,大多添加了大量的糖分、食用香精等添加剂来调味。另一方面,对于乳酸菌酸奶等液态产品还存在保质期短、贮藏运输成本高等缺点。再者,由于乳酸菌种类繁多,如何选择合适的菌种并进行合理搭配以达到更好的保健效果也是现今本领域的一个难点,因此研制一种菌种搭配合理、营养好、含糖量低、保质期长、质量稳定,并具有很好的保健效果的乳酸菌组合物是非常有必要的。
发明内容
本发明的目的在于针对现有技术中的不足而提供一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法,所制备的乳酸菌组合物原料配伍合理、营养好、含糖量低、保质期长、质量稳定,具有很好的保健效果的,而且制备工艺简单。
本发明的目的通过以下技术方案实现:
提供一种具有调节肠道菌群功能的乳酸菌组合物,由以下重量份的原料制成:
优选的,所述的一种具有调节肠道菌群功能的乳酸菌组合物,由以下重量份的原料制成:
更优选的,所述的一种具有调节肠道菌群功能的乳酸菌组合物,由以下重量份的原料制成:
优选的,所述乳酸菌组合物中益生菌的总浓度为5.0-8.0×109cfu/g。
本发明还提供上述一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,包括以下步骤:
步骤a、按重量份分别称取嗜酸乳杆菌干粉、嗜热链球菌干粉、罗伊氏乳杆菌干粉、乳双歧杆菌干粉、长双歧杆菌干粉、鼠李糖乳杆菌干粉、低聚果糖、低聚木糖和纳豆粉,混合均匀;
步骤b、将混合均匀的混合物过筛,得到所述乳酸菌组合物。
优选的,步骤b中,过筛的筛网孔径为80-100目。
本发明人结合各类型益生菌的安全性、定植、黏附力、抑菌、耐酸和耐胆盐能力等功能性特征,通过大量的研究与实验,确定了嗜酸乳杆菌、嗜热链球菌、罗伊氏乳杆菌、乳双歧杆菌、长双歧杆菌以及鼠李糖乳杆菌的益生菌组合,此外开创性地添加了活性纳豆粉。其中,乳杆菌和双歧杆菌是人类肠道重要的生理菌,对机体有多种其他正常生理菌无法比拟的生理作用,包括:调节肠道菌群、增强宿主肠道抵抗力、预防和治疗腹泻等等。嗜酸乳杆菌和鼠李糖乳杆菌属于乳杆菌,前者存在于非常严格厌氧环境的小肠部位,具有较强的耐酸及耐胆汁能力,能顺利通过肠胃环境而定植于大肠内;而鼠李糖乳杆菌在厌氧情况下能很好生长,肠道黏着率较高,定植能力强,其耐肠道环境的能力优于普通酸奶菌种和其它益生菌菌种,起到调节肠道菌群、预防和治疗腹泻等作用;嗜热链球菌作为潜在的有益菌,可到达小肠的上半部,有一定的胃肠道粘附性和转运活性;长双歧杆菌、乳双歧杆菌存在于大肠部,能粘附于宿主肠粘膜上皮细胞表面,形成稳定的菌群,起到生物屏障和生物拮抗作用。长双歧杆菌通过自身及产生的代谢产物排斥致病菌,在肠道中保持菌群优势,并与其它菌群相互作用,调整菌群间的关系,并维持菌群平衡。
此外,本发明配方中添加了纳豆粉,纳豆粉含有的活性纳豆菌进入肠道后纳豆菌生长过程中通过“生物夺氧”的方式消耗肠内过量的气体,形成厌氧环境并与有害细菌(如大肠杆菌)形成空间和营养的竞争,为益生菌在肠道内的定植创造了良好的生存环境,可以帮助益生菌顺利在肠道内定植,有助于益生菌的生长,纳豆菌和益生菌组合相辅相成,起协同作用,共同调节菌群,维持肠道微生态平衡,从而使失调的菌群平衡调整到正常的状态。
本发明的有益效果:
本发明的一种具有调节肠道菌群功能的乳酸菌组合物及其制备方法,其原料是由嗜酸乳杆菌干粉30-50份、嗜热链球菌干粉20-35份、罗伊氏乳杆菌干粉15-30份、乳双歧杆菌干粉30-40份、长双歧杆菌干粉25-35份、鼠李糖乳杆菌干粉15-25份、低聚果糖6-15份、低聚木糖5-10份和纳豆粉8-20份混合制成。本发明通过对肠道益生菌的研究以及大量的实验以及对比试验确定了最佳科学配比,上述原料组分相互配合,协同作用,调节机体肠道正常菌群,保持微生态平衡,恢复肠道正常的pH值等功能,本发明的乳酸菌组合物中益生菌总浓度达到5.0-8.0×109cfu/g。与现有技术相比,本发明具有以下优点:(1)本发明配方科学合理,各种组分相互促进,共同调节肠道菌群功能,比普通益生菌食品效果更好,而且该乳酸菌组合物在较长时间内稳定性好,保质期长,而且含糖量低,适合糖尿病人食用;(2)本发明在原料中添加了本身具有多重益生保健功能的纳豆粉,纳豆粉中的纳豆菌和其它益生菌相辅相成,共同调节肠道菌群,维持肠道微生态平衡;(3)本发明制备方法操作简便、运行可靠、生产效益高,适用于工业化生产。
具体实施方式
结合以下实施例对本发明作进一步描述。
实施例1:
本实施例的一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,包括以下步骤:
步骤a、按重量份分别称取嗜酸乳杆菌干粉40份、嗜热链球菌干粉25份、罗伊氏乳杆菌干粉20份、乳双歧杆菌干粉35份、长双歧杆菌干粉30份、鼠李糖乳杆菌干粉20份、低聚果糖9份、低聚木糖7份和纳豆粉10份,混合均匀;
步骤b、将混合均匀的混合物过筛,筛网孔径为80目,即得到所述乳酸菌组合物。
实施例2:
本实施例的一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,包括以下步骤:
步骤a、按重量份分别称取嗜酸乳杆菌干粉35份、嗜热链球菌干粉20份、罗伊氏乳杆菌干粉15份、乳双歧杆菌干粉30份、长双歧杆菌干粉35份、鼠李糖乳杆菌干粉15份、低聚果糖12份、低聚木糖5份和纳豆粉8份,混合均匀;
步骤b、将混合均匀的混合物过筛,筛网孔径为90目,即得到所述乳酸菌组合物。
实施例3:
本实施例的一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,包括以下步骤:
步骤a、按重量份分别称取嗜酸乳杆菌干粉45份、嗜热链球菌干粉30份、罗伊氏乳杆菌干粉25份、乳双歧杆菌干粉40份、长双歧杆菌干粉25份、鼠李糖乳杆菌干粉25份、低聚果糖6份、低聚木糖10份和纳豆粉15份,混合均匀;
步骤b、将混合均匀的混合物过筛,筛网孔径为100目,即得到所述乳酸菌组合物。
对比例1:
按重量份分别称取嗜酸乳杆菌干粉40份、嗜热链球菌干粉25份、罗伊氏乳杆菌干粉20份、乳双歧杆菌干粉35份、长双歧杆菌干粉30份、鼠李糖乳杆菌干粉20份、低聚果糖9份和低聚木糖7份,混合均匀后过筛,筛网孔径为80目,得到乳酸菌组合物。
对比例2:
按重量份称取纳豆粉10份。
对小鼠肠道有害菌菌群变化量的影响实验:
1、选取检疫合格的雄性Balb/c小鼠100只(SPF级),体重范围19.2~22.9g,实验动物按体重随机分为4组,即空白对照组(给予等量正常空白饲料)、实施例1-3组,每组25只,按20/kg灌胃给予相应受试物(正常对照组灌胃给予等量正常空白饲料),每日一次,连续给样30d。给予受试物前及末次给样24h后检测肠道菌群。
2、实验试剂:伊红美蓝琼脂、叠氮钠-结晶紫-七叶苷琼脂、双歧杆菌选择性培养基(BBL)、乳杆菌选择性培养基(Lbs)、胰胨-亚硫酸盐-环丝氨酸琼脂基础(TSC)、D-环丝氨酸溶液、甘油、生理盐水,低聚果糖,乙酸,吐温80。
在给药前,无菌采集小鼠粪便,加20%灭菌甘油生理盐水,以1:10稀释,制成均匀混悬液,1000转速离心,取上清,存于-80℃冰箱,待分析,最后一次给予组合物24h后,与实验前同样方式取直肠粪便,处理方法同上。
3、肠道菌实验样品处理方法:将采集到的肠道菌实验粪便样品,依次10倍递增稀释成一系列不同稀释度的含菌液至10-8,选择合适的稀释浓度分别接种在各培养基上。培养后,以菌落形态、格兰染色镜检、生化反应等鉴定计数菌落,计算出每克湿便中的菌落,取对数后进行统计学处理。肠道菌的培养条件及鉴定方法见表1。
表1.肠道菌的培养条件及鉴定方法
4、雄性Balb/c小鼠给予受试物前及末次给样后24h,按《保健食品检验与评价技术规范》2003版规定检测肠道菌群菌落数,连续两只小鼠的粪便放入同一无菌离心管,结果以菌落数的对数值(lg CFU)表示,并按规范规定的结果判定标准对实验结果进行分析,结果见表2。
表2.对小鼠肠道有害菌菌群变化量的影响
给药前各组间比较,肠杆菌、肠球菌、产气荚膜梭菌的数量差异均无显著性(t检验,*P>0.05)。
给药30d后各组与空白对照组比较,实施例1-3组的肠杆菌数量均显著低于空白对照组小鼠(t检验,*P<0.05)。给药30d后各组与空白对照组比较,实施例1-3的三组肠球菌数量均显著低于空白对照组小鼠(t检验,*P<0.05)。给药30d后各组与空白对照组比较,实施例1-3的三组产气荚膜梭菌数量均显著低于空白对照组小鼠(t检验,*P<0.05)。
表3.对小鼠肠道有益菌菌群变化量的影响
给药前各组之间比较,双歧杆菌、乳杆菌的数量差异均无显著性(t检验,*P>0.05)。给药30d后各组与空白对照组比较,实施例1-3组的双歧杆菌、乳杆菌数量显著高于空白对照组小鼠(t检验,*P<0.05)。
表4.对小鼠肠道菌群变化量(百分比)的影响
试验结果表明:按lg CFU计,给样前各组间小鼠粪便肠杆菌、肠球菌、产气荚膜梭菌、乳杆菌和双歧杆菌菌落数均无统计学差异。给样后,与空白对照组相比,实施例1、2的肠杆菌、肠球菌、产气荚膜梭菌显著降低,乳杆菌和双歧杆菌显著升高。对比实施例1和2的肠道菌群均有相同变趋势,其中实施例1的配方调理肠胃效果最好。对比没有添加纳豆粉的对比例1和只有纳豆粉的对比例2,实施例1和2的效果更好。这表明本发明的乳酸菌组合物配伍科学,所有组分在调节肠道菌群方面的确有协同作用,共同起调理肠道菌群的作用。
以上实验结果表明,本发明的乳酸菌组合物具有提高益生菌的数量,减少有害菌的数量,并能够改善肠道菌群结构的优点,具有广阔的应用前景。
本实施例仅用以说明本发明的技术方案,而非对本发明保护范围的限制,尽管参照较佳实施例对本发明作了详细地说明,本领域的普通技术人员应当理解,可以对本发明的技术方案进行修改或者等同替换,而不脱离本发明技术方案的实质和范围。
Claims (6)
1.一种具有调节肠道菌群功能的乳酸菌组合物,其特征在于:由以下重量份的原料制成:
2.根据权利要求1所述的一种具有调节肠道菌群功能的乳酸菌组合物,其特征在于:由以下重量份的原料制成:
3.根据权利要求2所述的一种具有调节肠道菌群功能的乳酸菌组合物,其特征在于:由以下重量份的原料制成:
4.根据权利要求1所述的一种具有调节肠道菌群功能的乳酸菌组合物,其特征在于:所述乳酸菌组合物中益生菌的总浓度为5.0-8.0×109cfu/g。
5.如权利要求1至4任意一项所述的一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,其特征在于:包括以下步骤:
步骤a、按重量份分别称取嗜酸乳杆菌干粉、嗜热链球菌干粉、罗伊氏乳杆菌干粉、乳双歧杆菌干粉、长双歧杆菌干粉、鼠李糖乳杆菌干粉、低聚果糖、低聚木糖和纳豆粉,混合均匀;
步骤b、将混合均匀的混合物过筛,得到所述乳酸菌组合物。
6.根据权利要求5所述的一种具有调节肠道菌群功能的乳酸菌组合物的制备方法,其特征在于:步骤b中,过筛的筛网孔径为80-100目。
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