CN109122425A - A method of the very wide water flea in the induction Pacific Ocean produces resting egg - Google Patents
A method of the very wide water flea in the induction Pacific Ocean produces resting egg Download PDFInfo
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- CN109122425A CN109122425A CN201811105423.4A CN201811105423A CN109122425A CN 109122425 A CN109122425 A CN 109122425A CN 201811105423 A CN201811105423 A CN 201811105423A CN 109122425 A CN109122425 A CN 109122425A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
Abstract
The invention discloses a kind of methods that the very wide water flea in the induction Pacific Ocean produces resting egg, the method steps are as follows: the culture solution of P latymonas subcordifomis and Skeletonema Costatum is obtained using culture medium culture, then again by micro algae culturing liquid and sterilizing seawater, and abscisic acid and acetylcholine be mixed into help lure to mix, obtain the culture solution of very wide water flea；Very wide water flea adult is put in a suitable place to breed in culture solution, then uses illumination, temperature and salinity combined stimulation, obtains very wide water flea resting egg.A kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg provided by the invention, strong operability, it is repeatable high, resting egg incidence is high, induced efficiency is high, this method makes full use of the measure of illumination, temperature and salinity combined stimulation, using progressive descending manner temperature-light stimulation method, obtains very wide water flea resting egg more, that energy stores are more sufficient.
Description
Technical field
The present invention relates to technical field of aquatic organism, produce resting egg more particularly, to a kind of very wide water flea in the induction Pacific Ocean
Method.
Technical background
Copepods is under the jurisdiction of Arthropoda, Crustachia, Copepoda, is the important component of zooplankter, is small
Type crustacean, body length < 3mm, battalion swims and parasitic life, in distribution Yu Haiyang, fresh water or brackish water.Because it is most fish
One of the open-mouthed bait of the class young, therefore be of great significance in aquatic ecosystem food web and aquaculture.The Pacific Ocean is very wide
Water flea is under the jurisdiction of calanoida, wide water flea section, very wide Daphnia, is the very common water warm bank Copepods species in less salt area
One of.Very wide water flea mainly appears on summer and autumn in the Pacific Ocean, starts to produce resting egg to autumn end, resting egg sinks to seabed and is embedded in sea
Mud and between continue hatching with recruit population.
Copepods is the key group of aquatic ecosystem, is risen emphatically in substance circulation, energy flow and information transmitting
It acts on.Resting egg is the important surviving policy of Copepods, is played in terms of resisting adverse environment, maintaining population important
Effect has a very important significance the season dynamic and species continuity of copepoda population.Resting egg is Copepods bad
The product of zoogamy continuity offspring is carried out under environmental condition, resting egg, which has, to be resisted extraneous poor environment, is easy to spread and lead to
The important provenance of the features such as recombination generates genetic diversity and the intensive culture of zooplankter is crossed, it is quick by Copepods
Single-female generation mass propagation in a short time, so that Copepods plays as the Seedling production that fresh food is aquatic economic animals
Extremely important effect.
It is also in culture fishery that the generation of resting egg, the formation of induced dormancy ovum and the in due course hatching of resting egg, which utilize,
Significantly project.Copepods is because it is rich in certain essential fatty acids, especially EPA and DHA in vivo, on aquatic fry growing
There are many applications, is frequently used to the open-mouthed bait as some economic fish.With the deeply hair with science and technology of research
Exhibition obtains a large amount of nauplius by Copepods resting egg and is gradually taken seriously and utilizes, and the scale evaluation of resting egg will be
It plays a significant role, has broad application prospects in modern sea-farming.
However the projects such as structure of research resting egg are then limited to only one season within 1 year and could acquire in sea area
Resting egg, and the rhythmicity of the very wide water flea production resting egg in the discovery Pacific Ocean is studied, mainly it is female for extraneous ring experienced
Border stimulation, such as temperature, periodicity of illumination and environment salinity external factor or its animal behavior such as property selection, parasitizing behavior, oviposition
Behavior etc., the influence to filial performance type otherness, therefore how to induce a large amount of resting eggs of production is a difficulty urgently to be resolved
Topic.
Summary of the invention
The purpose of the present invention is to provide a kind of strong operabilitys, and repeatability is high, and resting egg incidence is high, induced efficiency
Method that the high induction Pacific Ocean very wide water flea produces resting egg, this method make full use of illumination, temperature, salinity combined stimulation to arrange
It applies to complete high efficiency induction, using progressive descending manner temperature-light stimulation method, obtains more sufficient true of more, energy stores
Wide water flea resting egg.
The present invention in background technique aiming at the problem that mentioning, the technical solution taken are as follows:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, is by carrying out progressive decline to very wide water flea adult
Formula illumination, temperature and salinity combined stimulation, obtain and collect resting egg.This method is simple and convenient, make full use of illumination, temperature with
The measure of salinity combined stimulation induces to complete high efficiency, can be stimulated using progressive descending manner temperature-light stimulation method very wide
Water flea mother obtains higher resting egg incidence for zoogamy, realizes the purpose of quickly and efficiently induction production resting egg.
Preferably, the specific steps of progressive descending manner illumination, temperature and salinity combined stimulation are as follows:
Step 1: adjusting culture solution salinity is 2.8~3.1%, temperature be 24~27 DEG C, intensity of illumination be 2500~
Under conditions of 3000lux, light and shade time are 16h:8h, 3~5d is temporarily supported；
Step 2: culture solution salinity is adjusted to 3.3~3.6%, temperature be 18~22 DEG C, intensity of illumination be 1800~
2000lux cultivates 23~26h under conditions of the light and shade time is 12h:12h；
Step 3: culture solution salinity is adjusted to 3.3~3.6%, temperature be 15~18 DEG C, intensity of illumination be 1800~
2000lux cultivates 23~26h under conditions of the light and shade time is 12h:12h；
Step 4: culture solution salinity is adjusted to 3.3~3.6%, temperature be 12~16 DEG C, intensity of illumination be 1800~
2000lux, under conditions of the light and shade time is 12h:12h, culture to resting egg is generated.Illumination, temperature, water environment salinity, humidity,
The abiotic factors such as food have certain inducing action to the reproduction of zooplankter and the production of resting egg, such as higher
At a temperature of, the growth of very wide water flea is accelerated and promoted to very wide water flea the time needed for shortening sexal maturity, at accelerated development is known from experience
And reproduction, and lower temperature can induce the bisexual reproduction of Copepods；And other abiotic factors are also all to very wide water flea
Reproduction amount and resting egg incidence have apparent influence.
Preferably, population density of the very wide water flea adult in culture solution is 0.06~1.6/ml.Population density is
A key factor of the animal self-control to keep population stable, as population density increases, collision rate between very wide water flea
Increase, the probability for generating resting egg significantly increases, and controls population density in right amount, when being avoided that population density is excessive, can cause
Inanition and space it is crowded, and then avoid reducing its reproduction amount.
Preferably, the collection of resting egg is that the bolting silk for the use of aperture being 45~50 μm is collected.
Preferably, the very wide water flea in the induction Pacific Ocean produces in culture solution used in resting egg comprising by abscisic acid and acetyl gallbladder
Alkali composition helps lure.Abscisic acid and acetylcholine form high activity state, contain in molecule after receiving light stimulation
α and beta unsaturated ketone structure, be easy in conjunction with the hydrogen atom of Amino Acids in Proteins, and then with the diapause in oocyte membrane
Hormone receptor combines, and the activity of trehalase in oocyte membrane is improved, so that the trehalose in maternal blood be resolved into
Glucose, glucose enter egg mother cell by cell membrane, and a large amount of glycogen is synthesized in egg mother cell and is storeed, so that
Embryo of the fertilized eggs given birth to before the differentiation of embryo's corium is completed enters dormant state at initial stage, and the incidence of resting egg is caused to obtain
While further promotion, more sufficient energy can be stored for resting egg.
Further preferably, optimal technical solution is that help in lure the weight ratio of abscisic acid and acetylcholine be 2~2.5:
1.5。
Further preferably, the induction Pacific Ocean very wide water flea produce in culture solution used in resting egg also comprising P latymonas subcordifomis and
The culture solution of Skeletonema Costatum, preparation step are as follows: P latymonas subcordifomis and Skeletonema Costatum are inoculated on MS fluid nutrient medium,
Adjusting medium pH is 7.5~8.5, and salinity is 15~35 ‰, in temperature 20~30, DEG C 5000~10000lux of intensity of illumination
Under the conditions of, it cultivates to obtain the final product.Micro Algae not only size properly can by feeding of zooplankton, it is prior can be quick in animal body
It digests and assimilates, and nutrition rich in, facilitates the growth and development of animal, since many kinds of and its floating characteristics become floating
The object of looking for food of travelling object, by being ideal zooplankter bait after nutrition arrangement.
Further preferably, it includes following raw material that the very wide water flea in the induction Pacific Ocean, which produces culture solution used in resting egg: 5
~10 parts of P latymonas subcordifomis and Skeletonema Costatum culture solution, 20~25 parts of sterilizing seawater, 0.25~0.55 part help lure.
Still further preferably, sterilizing seawater is that the bolting silk for taking natural sea-water aperture to be 35~45 μm filters off quick sand and sundries
Afterwards, sterilizing and 20~25h of continuous charge to obtain the final product.
Compared with the prior art, the advantages of the present invention are as follows: 1) the provided in the present invention induction Pacific Ocean very wide water flea produce
The method of resting egg efficiently solves the acquisition problems of the very wide water flea resting egg in the Pacific Ocean, can be not only restricted to adopting for field sea area
The rhythmicity of collection and biology oviposition can obtain resting egg more, that energy stores are more sufficient；2) peaceful in induction in the present invention
During the very wide water flea in ocean produces resting egg, high efficiency induction is completed using the measure of illumination, temperature and salinity combined stimulation,
The zoogamy that very wide water flea mother generation is stimulated using progressive descending manner temperature-light stimulation method, obtains higher resting egg
Incidence realizes the purpose of quickly and efficiently induction production resting egg；3) the very wide water in the induction Pacific Ocean provided in the present invention
The method that flea produces resting egg is simple and convenient, strong operability, has repeatability, and resting egg incidence is high, and induced efficiency is high,
The resting egg of higher proportion can be obtained.
Specific embodiment
The present invention program is described further below by embodiment:
Embodiment 1:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, is by good through 2 domestication cultures from generation to generation, vigor
Very wide water flea adult carry out progressive descending manner illumination, temperature and salinity combined stimulation, obtain and collect resting egg.This method letter
Single convenient, strong operability makes full use of the measure of illumination, temperature and salinity combined stimulation to complete high efficiency induction, utilizes
Progressive descending manner temperature-light stimulation method can stimulate very wide water flea mother for zoogamy, obtain higher resting egg incidence,
Realize the purpose of quickly and efficiently induction production resting egg.
Progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 2.8%, temperature is 24 DEG C, intensity of illumination 2500lux, light and shade time are
Under conditions of 16h:8h, 3d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.3%, is 18 DEG C, intensity of illumination 1800lux in temperature, the light and shade time is
Under conditions of 12h:12h, 23h is cultivated；
Step 3: culture solution salinity being adjusted to 3.3%, is 15 DEG C, intensity of illumination 1800lux in temperature, the light and shade time is
Under conditions of 12h:12h, 23h is cultivated；
Step 4: culture solution salinity being adjusted to 3.3%, is 12 DEG C, intensity of illumination 1800lux in temperature, the light and shade time is
Under conditions of 12h:12h, culture to resting egg is generated.The abiotic factors pair such as illumination, temperature, water environment salinity, humidity, food
The reproduction of zooplankter and the production of resting egg have certain inducing action, such as at a higher temperature, very wide water flea at
Know from experience accelerated development, the time needed for shortening sexal maturity, accelerates and promote the growth and reproduction of very wide water flea, and lower temperature
Degree can induce the bisexual reproduction of Copepods；And also the reproduction amount all to very wide water flea and resting egg occur other abiotic factors
Rate has apparent influence.
Population density of the very wide water flea adult in culture solution is 0.06/ml.Population density be animal self-control with
A key factor for keeping population stable, as population density increases, collision rate increases between very wide water flea, generates resting egg
Probability significantly increase, and control population density in right amount, when being avoided that population density is excessive, can caused by inanition and space
It is crowded, and then avoid reducing its reproduction amount.
The collection of resting egg is that the bolting silk for the use of aperture being 45 μm is collected.
It includes to be made of in culture solution used in resting egg abscisic acid and acetylcholine that the very wide water flea in the induction Pacific Ocean, which produces,
Lure is helped, helping the weight ratio of abscisic acid and acetylcholine in lure is 2:1.5, and abscisic acid and acetylcholine are receiving light stimulation
Afterwards, high activity state, the α contained in molecule and beta unsaturated ketone structure are formed, is easy former with the hydrogen of Amino Acids in Proteins
Son combines, and then in conjunction with the diapause hormone receptor in oocyte membrane, improves the activity of trehalase in oocyte membrane,
To which the trehalose in maternal blood is resolved into glucose, glucose enters egg mother cell by cell membrane, in egg mother cell
It is middle to synthesize a large amount of glycogen and store, so that embryo initial stage of the fertilized eggs given birth to before the differentiation of embryo's corium is completed enters
Dormant state while causing the incidence of resting egg further to be promoted, can store more sufficient energy for resting egg.
Inducing the very wide water flea in the Pacific Ocean to produce also includes P latymonas subcordifomis and Skeletonema Costatum in culture solution used in resting egg
Culture solution, preparation step are as follows: P latymonas subcordifomis and Skeletonema Costatum are inoculated on MS fluid nutrient medium, adjust culture medium
PH is 7.5, and salinity is 15 ‰, under conditions of 20 DEG C of temperature, intensity of illumination 5000lux, is cultivated to obtain the final product.Micro Algae not only size
Properly can be prior to be absorbed in animal body by rapid digestion by feeding of zooplankton, and nutrition rich in, it helps
In the growth and development of animal, since many kinds of and its floating characteristics become the object of looking for food of zooplankter, by nutrition arrangement
It is ideal zooplankter bait later.
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 5 parts sub- heart-shaped flat
Algae and Skeletonema Costatum culture solution, 20 parts of sterilizing seawater, 0.25 part help lure.
Sterilizing seawater is after the bolting silk for taking natural sea-water aperture to be 35 μm filters off quick sand and sundries, and sterilize simultaneously continuous charge
20h to obtain the final product.
Embodiment 2:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, wherein the very wide water flea in the induction Pacific Ocean produces resting egg
Step are as follows: will be placed in culture solution through 2 good very wide water flea adults of domestication culture from generation to generation, vigor, very wide water flea adult is made to exist
Population density in culture solution is 0.26/ml, passes through progressive descending manner illumination, temperature and salinity combined stimulation in the training period
Measure, obtain simultaneously using aperture be 50 μm bolting silk collect resting egg.
Progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 3.0%, temperature is 27 DEG C, intensity of illumination 3000lux, light and shade time are
Under conditions of 16h:8h, 4d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.6%, is 20 DEG C, intensity of illumination 1900lux in temperature, the light and shade time is
Under conditions of 12h:12h, 25h is cultivated；
Step 3: culture solution salinity being adjusted to 3.6%, is 16 DEG C, intensity of illumination 1900lux in temperature, the light and shade time is
Under conditions of 12h:12h, 25h is cultivated；
Step 4: culture solution salinity being adjusted to 3.6%, is 14 DEG C, intensity of illumination 1900lux in temperature, the light and shade time is
Under conditions of 12h:12h, culture to resting egg is generated.
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 8 parts sub- heart-shaped flat
Algae and Skeletonema Costatum culture solution, 25 parts of sterilizing seawater, 0.50 part help lure, it is above-mentioned to help abscisic acid and acetylcholine in lure
Weight ratio is 2.5:1.5.
The culture solution preparation step of P latymonas subcordifomis and Skeletonema Costatum are as follows: be inoculated with P latymonas subcordifomis and Skeletonema Costatum
It is 8.5 on MS fluid nutrient medium, adjusting medium pH, salinity is 30 ‰, in 25 DEG C of temperature, the item of intensity of illumination 10000lux
Under part, cultivate to obtain the final product.
Sterilizing seawater is after the bolting silk for taking natural sea-water aperture to be 40 μm filters off quick sand and sundries, and sterilize simultaneously continuous charge
22h to obtain the final product.
Embodiment 3:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, wherein the very wide water flea in the induction Pacific Ocean produces resting egg
Step are as follows: will be placed in culture solution through 2 good very wide water flea adults of domestication culture from generation to generation, vigor, very wide water flea adult is made to exist
Population density in culture solution is 0.8/ml, passes through progressive descending manner illumination, temperature and salinity combined stimulation in the training period
Measure, obtain simultaneously using aperture be 48 μm bolting silk collect resting egg.
Progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 3.0%, temperature is 25 DEG C, intensity of illumination 2600lux, light and shade time are
Under conditions of 16h:8h, 3d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.5%, is 22 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 3: culture solution salinity being adjusted to 3.5%, is 18 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 4: culture solution salinity being adjusted to 3.5%, is 16 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, culture to resting egg is generated.
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 10 parts sub- heart-shaped flat
Algae and Skeletonema Costatum culture solution, 22 parts of sterilizing seawater, 0.35 part help lure, it is above-mentioned to help abscisic acid and acetylcholine in lure
Weight ratio is 2.3:1.5.
The culture solution preparation step of P latymonas subcordifomis and Skeletonema Costatum are as follows: be inoculated with P latymonas subcordifomis and Skeletonema Costatum
It is 8.0 on MS fluid nutrient medium, adjusting medium pH, salinity is 25 ‰, in 28 DEG C of temperature, the item of intensity of illumination 8000lux
Under part, cultivate to obtain the final product.
Sterilizing seawater is after the bolting silk for taking natural sea-water aperture to be 45 μm filters off quick sand and sundries, and sterilize simultaneously continuous charge
For 24 hours to obtain the final product.
Embodiment 4:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, advanced optimizes scheme are as follows:
When the very wide water flea production resting egg in the induction Pacific Ocean adjusts salinity with culture solution in step 4, added into culture solution
The glutamine that weight accounting is 0.065%, L-Glutamine and the weight ratio of D-Gln are 5:0.8 in glutamine,
The microalgae in culture solution is searched for food for a long time through very wide water flea at this time, be in the lower level of microalgae content, very wide water flea compared with
It can be in starvation in the short time, cause very wide water flea intestinal mucosal permeability to increase, the glutamine of the proportion is added
In culture solution, enteron aisle can be stimulated to keep mucous membrane structure, enhancing intestinal cell activity promotes insulin and testosterone secretion to increase,
Promote the zoogamy of adult, while can be formed and be coupled with antifreeze protein and opsin, period internal stimulus parent is quick looking after
Reach Diapause inducement sensitive periods, release more diapause hormones, mother is converted to diapause for received extraneous pessimal stimulation signal
Signal directly acts on filial generation, so that induced efficiency improves, and have stimulated more, the more stable resting egg of female generation generation.
The step of inducing the very wide water flea in the Pacific Ocean to produce resting egg are as follows: by the very wide water good through 2 domestication cultures from generation to generation, vigor
Flea adult is placed in culture solution, is made population density 0.8/ml of the very wide water flea adult in culture solution, is led in the training period
Progressive descending manner illumination, the measure of temperature and salinity combined stimulation are crossed, the bolting silk collection suspend mode for obtaining and the use of aperture being 48 μm
Ovum.
Progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 3.0%, temperature is 25 DEG C, intensity of illumination 2600lux, light and shade time are
Under conditions of 16h:8h, 3d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.5%, is 22 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 3: culture solution salinity being adjusted to 3.5%, is 18 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 4: culture solution salinity being adjusted to 3.5%, is 16 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, culture to resting egg is generated.
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 10 parts sub- heart-shaped flat
Algae and Skeletonema Costatum culture solution, 22 parts of sterilizing seawater, 0.35 part help lure, it is above-mentioned to help abscisic acid and acetylcholine in lure
Weight ratio is 2.3:1.5.
The culture solution preparation step of P latymonas subcordifomis and Skeletonema Costatum are as follows: be inoculated with P latymonas subcordifomis and Skeletonema Costatum
It is 8.0 on MS fluid nutrient medium, adjusting medium pH, salinity is 25 ‰, in 28 DEG C of temperature, the item of intensity of illumination 8000lux
Under part, cultivate to obtain the final product.
Sterilizing seawater is after the bolting silk for taking natural sea-water aperture to be 45 μm filters off quick sand and sundries, and sterilize simultaneously continuous charge
For 24 hours to obtain the final product.
Comparative example:
A method of the very wide water flea in the induction Pacific Ocean produces resting egg, wherein the very wide water flea in the induction Pacific Ocean produces resting egg
Step are as follows: will be placed in culture solution through 2 good very wide water flea adults of domestication culture from generation to generation, vigor, very wide water flea adult is made to exist
Population density in culture solution is 0.8/ml, passes through progressive descending manner illumination, temperature and salinity combined stimulation in the training period
Measure, obtain simultaneously using aperture be 48 μm bolting silk collect resting egg.
Progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 3.0%, temperature is 25 DEG C, intensity of illumination 2600lux, light and shade time are
Under conditions of 16h:8h, 3d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.5%, is 22 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 3: culture solution salinity being adjusted to 3.5%, is 18 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, cultivation is for 24 hours；
Step 4: culture solution salinity being adjusted to 3.5%, is 16 DEG C, intensity of illumination 2000lux in temperature, the light and shade time is
Under conditions of 12h:12h, culture to resting egg is generated.
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 10 parts sub- heart-shaped flat
Algae and Skeletonema Costatum culture solution, 22 parts of sterilizing seawater.
In this comparative example, the very wide water flea in the induction Pacific Ocean, which produces to be not added in culture solution used in resting egg, helps lure, other
In the same manner as in Example 3, very wide water flea resting egg is made.
Embodiment 5:
The very wide water flea resting egg in the Pacific Ocean induces pilot production
The very wide water flea adult good through 2 domestication cultures from generation to generation, vigor is chosen, respectively according to Examples 1 to 4 and comparative example
Condition be placed in 5 culture ponds, while one group of blank group is set, takes that normal light shines, salinity and temperature condition are trained
It educates.In the nurturing period, each experimental condition of strict control, when detecting breeding parent number less than 2, off-test.Experiment knot
Fruit statistics is as follows.
The very wide water flea resting egg in 1 Pacific Ocean of table induces pilot production
| Male body occurrence rate/% | Resting egg incidence/% | Normal ovum incidence/% | |
| Embodiment 1 | 40.7 | 64.5 | 35.5 |
| Embodiment 2 | 40.6 | 64.7 | 35.3 |
| Embodiment 3 | 42.7 | 65.3 | 34.7 |
| Embodiment 4 | 43.3 | 65.5 | 34.5 |
| Comparative example | 37.8 | 57.3 | 42.7 |
| Blank group | 33.3 | 50.5 | 49.5 |
By upper Biao Ke get, the male body occurrence rate of very wide water flea obtained by Examples 1 to 4 is apparently higher than comparative example, especially in fact
It applies and is higher than comparative example 13.7%, also significantly greater than blank group 29.1% obtained by example 3 and 4；The resting egg incidence of Examples 1 to 4
Difference is little, is averagely higher than comparative example 7.7%, is higher than blank group 15%；There is experimental result that can obtain, is given birth in the embodiment of the present invention
For the resting egg incidence of production obviously by progressive descending manner illumination, the combined stimulation of temperature and salinity, resting egg incidence is obvious
Improve, especially embodiment 3 and 4, while also by helping lure to be significantly affected, illustrate in the present invention using illumination, temperature with
The measure of salinity combined stimulation can complete the induction to very wide water flea resting egg, while can obtain higher resting egg incidence,
Resting egg more, that energy stores are more sufficient is obtained, realizes the purpose of quickly and efficiently induction production resting egg.
Routine operation in operation of the present invention step is well known to those skilled in the art, herein without repeating.
Technical solution of the present invention is described in detail in embodiment described above, it should be understood that the above is only
For specific embodiments of the present invention, it is not intended to restrict the invention, all any modifications made in spirit of the invention,
Supplement or similar fashion substitution etc., should all be included in the protection scope of the present invention.
Claims (9)
1. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg, it is characterised in that: by being carried out to very wide water flea adult
Progressive descending manner illumination, temperature and salinity combined stimulation, obtain and collect resting egg；
The progressive descending manner illumination, temperature and salinity combined stimulation specific steps are as follows:
Step 1: adjusting culture solution salinity is 2.8 ~ 3.1%, temperature is 24 ~ 27 DEG C, intensity of illumination is 2500 ~ 3000lux, bright
Under conditions of the dark time is 16h:8h, 3 ~ 5d is temporarily supported；
Step 2: culture solution salinity being adjusted to 3.3 ~ 3.6%, is 18 ~ 22 DEG C in temperature, intensity of illumination is 1800 ~ 2000lux, bright
Under conditions of the dark time is 12h:12h, 23 ~ 26h is cultivated；
Step 3: culture solution salinity being adjusted to 3.3 ~ 3.6%, is 15 ~ 18 DEG C in temperature, intensity of illumination is 1800 ~ 2000lux, bright
Under conditions of the dark time is 12h:12h, 23 ~ 26h is cultivated；
Step 4: culture solution salinity being adjusted to 3.3 ~ 3.6%, is 12 ~ 16 DEG C in temperature, intensity of illumination is 1800 ~ 2000lux, bright
Under conditions of the dark time is 12h:12h, culture to resting egg is generated.
2. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 1, it is characterised in that: described
Population density of the very wide water flea adult in culture solution is 0.06 ~ 1.6/ml.
3. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 1, it is characterised in that: described
The collection of resting egg is that the bolting silk for the use of aperture being 45 ~ 50 μm is collected.
4. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 1, it is characterised in that: described
Very wide water flea produces in culture solution used in resting egg comprising helping lure by what abscisic acid and acetylcholine formed in the induction Pacific Ocean.
5. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 4, it is characterised in that: described
Helping the weight ratio of abscisic acid and acetylcholine in lure is 2 ~ 2.5:1.5.
6. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 4, it is characterised in that: described
Inducing the very wide water flea in the Pacific Ocean to produce also includes the culture solution of P latymonas subcordifomis and Skeletonema Costatum in culture solution used in resting egg.
7. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 4, it is characterised in that: described
The culture solution preparation condition of P latymonas subcordifomis and Skeletonema Costatum are as follows: MS fluid nutrient medium is used, medium pH is 7.5 ~ 8.5,
Salinity is 15 ~ 35 ‰, and cultivation temperature is 20 ~ 30 DEG C, and intensity of illumination is 5000 ~ 10000lux.
8. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 4, it is characterised in that: described
Inducing the very wide water flea in the Pacific Ocean to produce culture solution used in resting egg includes following raw material: 5 ~ 10 parts of P latymonas subcordifomis are in
Skeletonemacostatum culture solution, 20 ~ 25 parts of sterilizing seawater, 0.25 ~ 0.55 part help lure.
9. a kind of method that the very wide water flea in the induction Pacific Ocean produces resting egg according to claim 8, it is characterised in that: described
Sterilizing seawater be after the bolting silk for taking natural sea-water aperture to be 35 ~ 45 μm filters off quick sand and sundries sterilize and continuous charge 20 ~
25h to obtain the final product.
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| 刘光兴等: "海洋桡足类滞育卵在海水养殖业中的应用及其前景", 《青岛海洋大学学报》 * |
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| 王庆等: "河口近海桡足类休眠卵生态学研究进展", 《应用生态学报》 * |
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