CN101658147A - Method for improving survival rate of pomfret fry - Google Patents
Method for improving survival rate of pomfret fry Download PDFInfo
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- CN101658147A CN101658147A CN200810042030A CN200810042030A CN101658147A CN 101658147 A CN101658147 A CN 101658147A CN 200810042030 A CN200810042030 A CN 200810042030A CN 200810042030 A CN200810042030 A CN 200810042030A CN 101658147 A CN101658147 A CN 101658147A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for improving survival rate of pomfret fry, relates to breeding of pomfret fry, and needs to provide a method for improving survival rate and growth speed of pomfret fry. The method comprises the following steps: measuring a DHA/EPA/ARA proportion in pomfret parent fish tissues and body fatty acid compositions of the pomfret fry, wherein the step is characterized by mixing cuttlefish liver oil, refined fish oil, doxicon and peanut oil to form emulsified oil of which DHA/EPA/ARA proportion is 2.5/1.0/0.2; adding 0.25 gram, 0.35 gram and 0.45 gram of the emulsified oil into the breeding water in terms of each liter of rotifers to reinforce the rotifers respectively; and feeding the pomfret fry with the rotifers reinforced by adopting the best DHA/EPA/ARA allocation proportion reinforcing baits from the 4th day to the 12th day under the illumination condition of 1,000 to 50,001x at the water temperature of between 20 and 24 DEG C in the water with pomfretfry breeding density of 15,000 to 20,000 fishes per cube. The method is used for breeding the pomfret fry.
Description
Technical field:
The present invention relates to the pomfret fry breeding method.
Background technology
In seawater fish artificial seed cultivating process, the level of necessary high unsaturated fatty acid is one of principal element that influences seedling early growth speed and survival rate height in the nutrition supply of prelarva, particularly marine fish young growth and development process.The different marine fish young and the different developmental phases young of marine fish of the same race are to the demand difference of the high unsaturated fatty acid in the bait.
Studies show that the optimal proportion of DHA/EPA/ARA is 1.8/1.0/0.12 in the turbot prelarva early development.With DHA/EPA is that 2/1 reinforcement living bait is cultivated the young postlarva of turbot, has prevented the generation of turbot albinism effectively.
The optimal proportion of DHA/EPA is approximately 2/1 in the feed of the young postlarva of perch, and the best proportioning of EPA/ARA is approximately 1/1.
The ratio of the best DHA/EPA/ARA of haddock young bait is 10/1/1.
The optimal proportion of EPA and ARA is 2/1 in the flounder baby fish experiment particulate feed.
The ratio of DHA/EPA is 2.31 o'clock in the bait wheel polypide of the golden head porgy young, and the growth rate of golden head porgy prelarva is the fastest.
Above-mentioned studies show that, there is notable difference in the different seawater fish young to the demand of the ratio of DHA/EPA/ARA in the bait, and at different cultured fishes, it is necessary selecting fatty acid to form appropriate H UFA hardening agent.
Silvery pomfret has very high economic worth, and the silvery pomfret growth just can reach commercial specification rapidly then in natural waters, has good DEVELOPMENT PROSPECT as culturing new varieties.Still there is ample room for improvement for the survival rate of pomfret fingerlings (the silvery pomfret young of 3-12 age in days) cultivation at present and growth rate.But in growing seedlings, silvery pomfret the research of fatty acid demand in the bait be yet there are no report about pomfret fry.In seed produces, just be used to the fry of throwing something and feeding usually, go back the ratio of neither one at the DHA/EPA/ARA of the reinforcement bait that improves pomfret fry growth rate and survival rate with the wheel animalcule of marine chlorella or fish oil hot housing.
Summary of the invention
The technical issues that need to address of the present invention be the silvery pomfret young by exploring the 3-12 age in days to the demand of DHA/EPA/ARA ratio in the bait, a kind of method that improves survival rate of pomfret fry and growth rate is provided.
Technical scheme of the present invention adopts soma of the ripe silvery pomfret parent population of mensuration natural waters and fat content, the fatty acid percentage composition of the natural waters pomfret young, the ratio of DHA/EPA/ARA during body fat acid is formed, it is characterized in that be raw material according to soma of silvery pomfret parent population and fat content, the fatty acid percentage composition measurement result of the pomfret young with inkfish liver oil, refined fish oil, polyenoid health and peanut oil, be mixed and made into the oil emulsion of DHA/EPA/ARA=2.5/1.0/0.2 ratio; Cultivate the dosage that adds 0.25g, 0.35g and 0.45g in the water body with above-mentioned oil emulsion by every liter of wheel animalcule and strengthened brachionus plicatilis respectively 12 hours, survival rate that the statistics wheel animalcule is strengthened and the fat content of measuring wheel animalcule and fatty acid are formed; Cultivating density at pomfret fry is 15000-20000 tail/cube water body, under illumination 1000-5000lx, water temperature 20-24 ℃ the condition, by identical management condition each group is strengthened back wheel animalcule silvery pomfret 3～12 age in days prelarvas of throwing something and feeding respectively, the good wheel animalcule of primary reinforcement of throwing something and feeding on per 4 hours of daytime, each wheel animalcule 5～15/ml of density that throws something and feeds; Measuring different survival rate and the long growth of body, body fat and the fatty acid of strengthening the pomfret fry of bait cultivation when prelarva 13 ages in days forms; Survival rate, body according to the pomfret fry of strengthening the wheel animalcule nursing increase and the fatty acid composition, determine the best allotment ratio of strengthening bait of 3～12 age in days pomfret fries; Throwing something and feeding for continuously the silvery pomfret seedling from the 4th day to 12 days adopts the best allotment ratio of DHA/EPA/ARA to strengthen the wheel animalcule that bait is strengthened.
The present invention compares with conventional seedbed system with the 3-12 age in days pomfret fry of throwing something and feeding of the wheel animalcule after strengthening, and the growth rate of pomfret fry improves 2.6%, and survival rate improves more than 10%.
Embodiment
The specific embodiment of the present invention is carried out according to the following steps:
(1) gathers the gonadal tissue and the natural waters pomfret young of the ripe silvery pomfret parent population of natural waters respectively, adopt the chloroform methanol method to measure the fat content of soma, adopt gas chromatograph, with the fatty acid standard items is reference, calculate the fatty acid percentage composition of the sample of respectively originating with normalization method, and the difference of dividing the different sample samples of folding, drawing the normal body fat content of the early stage larval stages of silvery pomfret is 9.98%, DHA/EPA/ARA=3.0/1.0/0.19 during body fat acid is formed;
(2) according to the measurement result of step (1), be raw material, be mixed and made into the DHA/EPA/AEA ratio and strengthen bait for the oil emulsion of=2.5/1.0/0.2 with inkfish liver oil, refined fish oil, polyenoid health (being rich in DHA and EPA) and peanut oil;
(3) cultivate the dosage that adds 0.25g, 0.35g and 0.45g in the water body with above-mentioned oil emulsion by every liter of wheel animalcule and strengthened fold arm tail rifle worm respectively 12 hours, the density of wheel animalcule is 500/milliliter, and survival rate that the statistics wheel animalcule is strengthened and the fat content of measuring wheel animalcule and fatty acid are formed;
(4) pomfret fry cultivation density is 15000-20000 tail/cube water body; Under illumination 1000-5000lx, water temperature 21-24 ℃ condition, each group is strengthened back wheel animalcule silvery pomfret 3-12 age in days prelarva of throwing something and feeding respectively, the good wheel animalcule of primary reinforcement of throwing something and feeding on per 4 hours of daytime, the each wheel animalcule density 5-15/ml that throws something and feeds; Survival rate and the body length of measuring the pomfret fry of difference reinforcement bait cultivation when prelarva 13 ages in days increase, and the body fat and the fatty acid of the pomfret fry of analysis difference reinforcement bait cultivation are formed;
(5) survival rate, the body of forming and strengthen the pomfret fry that wheel animalcule feeds according to the survival rate of strengthening wheel animalcule, fatty acid increases and fatty acid is formed, determine that the best bait of strengthening of 3～12 age in days pomfret fries is formed by inkfish liver oil, refined fish oil and the allotment of polyenoid health, the DHA/EPA/ARA ratio that bait is strengthened in the allotment back is 2.5/1/0.2, cultures the best results of 3～12 age in days pomfret fries;
The wheel animalcule of throwing something and feeding continuously and strengthening through the reinforcement bait of DHA/EPA/ARA=2.5/1/0.2 from the 4th day to 12 days is compared with conventional breeding method after throwing something and feeding 13 days, and the prelarva growth rate improves 2.6%, and survival rate improves 10%.
Claims (1)
1, improves the method for survival rate of pomfret fry, measure soma of the ripe silvery pomfret parent population of natural waters and fat content, the fatty acid percentage composition of the natural waters pomfret young, the ratio of DHA/EPA/ARA is characterized in that be the oil emulsion that mixed raw material becomes the DHA/EPA/ARA=2.5/1.0/0.2 ratio according to soma of silvery pomfret parent population and fat content, the fatty acid percentage composition measurement result of the pomfret young with inkfish liver oil, refined fish oil, polyenoid health and peanut oil during body fat acid was formed; Cultivate the dosage that adds 0.25g, 0.35g and 0.45g in the water body with above-mentioned oil emulsion by every liter of wheel animalcule and strengthened brachionus plicatilis respectively 12 hours, survival rate that the statistics wheel animalcule is strengthened and the fat content of measuring wheel animalcule and fatty acid are formed; Cultivating density at pomfret fry is 15000-20000 tail/cube water body, under illumination 1000-5000lx, water temperature 20-24 ℃ the condition, by identical management condition each group is strengthened back wheel animalcule silvery pomfret 3～12 age in days prelarvas of throwing something and feeding respectively, the good wheel animalcule of primary reinforcement of throwing something and feeding on per 4 hours of daytime, each wheel animalcule 5～15/ml of density that throws something and feeds; Measuring different survival rate and the long growth of body, body fat and the fatty acid of strengthening the pomfret fry of bait cultivation when prelarva 13 ages in days forms; Survival rate, body according to the pomfret fry of strengthening the wheel animalcule nursing increase and the fatty acid composition, determine the best allotment ratio of strengthening bait of 3～12 age in days pomfret fries; Throwing something and feeding for continuously the silvery pomfret seedling from the 4th day to 12 days adopts the best allotment ratio of DHA/EPA/ARA to strengthen the wheel animalcule that bait is strengthened.
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| CN200810042030.3A CN101658147B (en) | 2008-08-25 | 2008-08-25 | Method for improving survival rate of pomfret fry |
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| CN200810042030.3A CN101658147B (en) | 2008-08-25 | 2008-08-25 | Method for improving survival rate of pomfret fry |
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| CN101658147A true CN101658147A (en) | 2010-03-03 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859218A (en) * | 2014-03-17 | 2014-06-18 | 中国农业科学院饲料研究所 | Starter feed for promoting growth of sturgeon larvae and improving survival rate of sturgeon larvae |
| CN106342731A (en) * | 2016-08-29 | 2017-01-25 | 中国水产科学研究院东海水产研究所 | Wild pampus argenteus seedling acclimatizing method |
| CN110999836A (en) * | 2019-12-31 | 2020-04-14 | 海南晨海水产有限公司 | Nutrition strengthening method for promoting growth of penaeus orientalis larvae |
| CN111280091A (en) * | 2018-12-09 | 2020-06-16 | 宁波大学 | Pomfret ecological seedling raising method |
| CN113016669A (en) * | 2021-03-22 | 2021-06-25 | 浙江省海洋水产研究所 | Domestication method for pomfret to completely ingest pellet feed |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101167443B (en) * | 2006-10-27 | 2010-05-12 | 中国水产科学研究院东海水产研究所 | Pomfret artificial insemination and hatching method |
| CN101167442B (en) * | 2006-10-27 | 2010-05-12 | 中国水产科学研究院东海水产研究所 | Pomfret fingerlings artificial culturing method |
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2008
- 2008-08-25 CN CN200810042030.3A patent/CN101658147B/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103859218A (en) * | 2014-03-17 | 2014-06-18 | 中国农业科学院饲料研究所 | Starter feed for promoting growth of sturgeon larvae and improving survival rate of sturgeon larvae |
| CN103859218B (en) * | 2014-03-17 | 2015-10-28 | 中国农业科学院饲料研究所 | A kind of starter diet promoting sturgeon Larval and improve survival rate |
| CN106342731A (en) * | 2016-08-29 | 2017-01-25 | 中国水产科学研究院东海水产研究所 | Wild pampus argenteus seedling acclimatizing method |
| CN106342731B (en) * | 2016-08-29 | 2019-04-26 | 中国水产科学研究院东海水产研究所 | A kind of acclimation method of silvery pomfret fish wild seed |
| CN111280091A (en) * | 2018-12-09 | 2020-06-16 | 宁波大学 | Pomfret ecological seedling raising method |
| CN111280091B (en) * | 2018-12-09 | 2022-02-15 | 宁波大学 | A kind of silver pomfret ecological seedling raising method |
| CN110999836A (en) * | 2019-12-31 | 2020-04-14 | 海南晨海水产有限公司 | Nutrition strengthening method for promoting growth of penaeus orientalis larvae |
| CN113016669A (en) * | 2021-03-22 | 2021-06-25 | 浙江省海洋水产研究所 | Domestication method for pomfret to completely ingest pellet feed |
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Granted publication date: 20140122 Termination date: 20170825 |