CN109116013B

CN109116013B - Multiple immunity analyzer

Info

Publication number: CN109116013B
Application number: CN201811115598.3A
Authority: CN
Inventors: 柳邦源; 林艳; 向裕; 罗全胜; 郑岳生
Original assignee: Zhuhai Livzon Diagnostics Inc
Current assignee: Zhuhai Livzon Diagnostics Inc
Priority date: 2018-09-25
Filing date: 2018-09-25
Publication date: 2021-07-20
Anticipated expiration: 2038-09-25
Also published as: CN109116013A

Abstract

The invention relates to the technical field of medical detection equipment, in particular to a multiple immunity analyzer, which comprises a rack, a first picking and placing mechanism, an automatic conveying device, a blending device, an incubation oscillating device, a magnetic sheet tiling device, a transfer mechanism and a second picking and placing mechanism, wherein the automatic conveying device is used for conveying a reaction tube for an experiment, the experimental specimen which is respectively arranged on the first taking and placing mechanism, the diluent which is arranged on the second taking and placing mechanism and the reagent which is arranged on the blending device are injected into the reaction tube through the transfer mechanism, the temperature and the mixing condition required by the reaction of the experimental sample and the reagent are provided by the incubation oscillating mechanism for incubation, and then the magnetic sheet is tiled at the bottom of the reaction tube by the magnetic sheet tiling device, so that the detection accuracy is improved.

Description

Multiple immunity analyzer

Technical Field

The invention relates to the technical field of medical detection equipment, in particular to a multiple immunity analyzer.

Background

At present, for the detection experiment of the multiple methods, the laboratory also depends on manual operation and instruments with different functions to complete the requirements of each step of the experiment, which necessarily causes low experiment efficiency, inaccurate control of each process, inaccurate interpretation result and large waste of manpower and material resources, so that an instrument capable of automatically carrying out the multiple method experiment, accurately controlling the requirements in each step of the experiment and obtaining an accurate interpretation result is urgently needed.

Disclosure of Invention

The technical scheme adopted by the invention to solve the problems is to provide a multiple immunity analyzer, which comprises a frame and also comprises: the first taking and placing mechanism can take and place the experimental specimen; an automatic transfer device capable of transferring the reaction tube; a mixing device capable of mixing reagents; the incubation oscillation device can oscillate and uniformly mix the experimental sample and the reagent and provide the temperature required by the reaction of the experimental sample and the reagent; a transfer mechanism capable of transferring the reagent and the detection solution; and the second taking and placing mechanism can take and place the diluent.

As a further improvement of the scheme, the automatic conveying device comprises an X-direction pushing mechanism for pushing the reaction tube to move along the X direction and a Y-direction pushing mechanism for pushing the reaction tube to move along the Y direction.

As a further improvement of the above scheme, the Y-direction pushing mechanism includes a second pushing block capable of moving along the Y-direction and the Z-direction, and the second pushing block is provided with an elastic pressing block.

As a further improvement of the above scheme, the blending device comprises a rotatable reagent turntable and a reagent seat eccentrically arranged on the reagent turntable, wherein the reagent seat can rotate, and a connecting seat capable of rotating is arranged in the reagent seat.

As a further improvement of the scheme, a first transmission gear is fixedly arranged in the reagent rotating disc, the connecting seat is connected with a second transmission gear meshed with the first transmission gear, and when the connecting seat rotates along with the reagent seat, the second transmission gear is meshed with the first transmission gear to rotate so as to drive the connecting seat to rotate.

As a further improvement of the above scheme, the incubation oscillation device comprises a rotation source, an eccentric part arranged at an output end of the rotation source and a first tray arranged on the rotation source, wherein the rotation source drives the eccentric part to rotate, and the first tray oscillates along the circumferential direction along with the eccentric part.

As a further improvement of the above scheme, the incubation oscillation device further includes a heat-generating incubation mechanism capable of accommodating the first tray, and an X-direction displacement mechanism for driving the first tray to move.

As a further improvement of the scheme, the device also comprises a magnetic sheet spreading device which can spread the magnetic sheet in the detection liquid at the bottom of the reaction tube.

As a further improvement of the scheme, the magnetic sheet flatly-laying device comprises an eccentric rotating shaft and a second tray arranged on the upper portion of the eccentric rotating shaft, and the eccentric rotating shaft drives the second tray to swing along the circumferential direction.

As a further improvement of the scheme, the magnetic sheet tiling device further comprises a second base fixedly installed and a reset assembly arranged on the second base, and the upper end of the reset assembly is abutted to the second tray.

The invention has the beneficial effects that: according to the invention, the reaction tube is conveyed for an experiment through the automatic conveying device, the experiment sample, the diluent and the reagent are respectively injected into the reaction tube, the incubation oscillating mechanism is used for providing the temperature and the mixing condition required by the reaction of the experiment sample and the reagent for incubation, and the magnetic sheet is flatly laid at the bottom of the reaction tube through the magnetic sheet flatly laying device, so that the detection accuracy is improved.

Drawings

The invention is further illustrated by the following figures and examples.

FIG. 1 is an exploded view of a multiple immunoassay analyzer of the present invention;

FIG. 2 is a top view of a multiple immunoassay analyzer of the present invention;

fig. 3 is an exploded view of the first pick and place mechanism of the present invention;

FIG. 4 is a side view of the structure of the automatic transfer device of the present invention;

FIG. 5 is a two-dimensional front view of the automated transfer device of the present invention;

FIG. 6 is a two-dimensional cross-sectional view of an automated transfer device of the present invention;

FIG. 7 is a side view showing the structure of a Y-direction pushing mechanism of the automatic transfer device of the present invention;

FIG. 8 is an exploded view of the blending apparatus of the present invention;

FIG. 9 is a two-dimensional bottom view of the blending apparatus of the present invention;

FIG. 10 is a sectional view showing the structure of the kneading apparatus of the present invention;

FIG. 11 is an exploded view of the incubation oscillation device of the present invention;

FIG. 12 is an exploded view of the oscillating mechanism of the incubation oscillating device of the present invention;

FIG. 13 is a sectional view of a two-dimensional structure of an incubation oscillation device of the present invention;

FIG. 14 is an exploded view of the washing mechanism of the present invention;

fig. 15 is an exploded view of the magnetic sheet tiling apparatus of the present invention;

fig. 16 is a schematic structural view of a reset assembly of the magnetic sheet tiling apparatus of the present invention;

figure 17 is a side view of the configuration of the magnetic sheet tiling apparatus of the present invention;

FIG. 18 is an exploded view of the transfer mechanism of the present invention;

FIG. 19 is an exploded view of the card pushing mechanism of the present invention;

fig. 20 is an exploded view of the second pick and place mechanism of the present invention;

FIG. 21 is a three-dimensional side view of a reaction tube of the present invention;

FIG. 22 is a two-dimensional front view of a reaction tube of the present invention;

FIG. 23 is a two-dimensional plan view of a reaction tube of the present invention.

Detailed Description

The invention will now be described in further detail with reference to the drawings and preferred embodiments.

As shown in fig. 1, the multiple immunity analyzer includes a frame and an electrical control module, and further includes a first pick-and-place mechanism 1, an automatic transmission device 2, a blending device 3, an incubation oscillation device 4, a washing mechanism 5, an interpretation module 6, a magnetic sheet laying device 7, a transfer mechanism 8, a card pushing mechanism 10 and a second pick-and-place mechanism 11, which are respectively disposed on the frame, specifically, as shown in fig. 2, the automatic transmission device 2 is disposed at the front end of the frame, the first pick-and-place mechanism 1 and the second pick-and-place mechanism 11 are disposed side by side at the front end of the automatic transmission device 2, the blending device 3 is disposed at the tail end of the automatic transmission device 2, the washing mechanism 5 is disposed at the right rear end of the frame, the incubation oscillation device is disposed between the blending device 3 and the washing mechanism 5, and the other expression is that the first pick-and-place mechanism 1 and the incubation oscillation device 4 are respectively disposed at the left and right ends of the frame, the interpretation module 6 is arranged at the rear end of the rack, the card pushing mechanism 10 is arranged in the area between the automatic conveying device 2 and the interpretation module 6 on the rack, and the magnetic sheet tiling device 7 is arranged at the left side of the rear end of the rack.

As shown in fig. 3, the first pick-and-place mechanism 1 is used for placing an experimental specimen and scanning and reading information of the experimental specimen, and includes a first base 101 fixedly mounted on the rack, a specimen rack 102 detachably mounted on the first base 101, a dragging assembly 103 and a scanning assembly 104 arranged at the rear end of the first base 101, the first base 101 is provided with a plurality of sliding grooves 105, the specimen rack 102 can slide along the sliding grooves 105, in this embodiment, the first base 101 can be simultaneously detachably mounted with five specimen racks 102, each specimen rack 102 can be used for placing eight specimen test tubes, it can also adjust the number of the specimen racks 102 detachably mounted on the first base 101 and the number of the specimen test tubes that can be placed on the specimen racks 102 at the same time according to actual requirements, the head end of each sliding groove 105 is provided with a travel switch 106, and the travel switch 106 can specifically include a proximity sensor and a signal lamp, the travel switch 106 is used for detecting whether the specimen holder 102 is placed on the sliding chute 105, and if the specimen holder 102 is placed on the sliding chute 105, the proximity sensor generates a detection signal and the signal lamp is turned on; the dragging component 103 comprises a first linear sliding table component capable of sliding transversely and longitudinally and a grabbing component 107 for grabbing the specimen holder 102, the first linear sliding table component can slide transversely and longitudinally through two linear sliding tables which are perpendicular to each other, the grabbing component 107 is arranged on the first linear sliding table component and can move along with the first linear sliding table component, the grabbing component 107 can be a clamping jaw or can grab through a magnetic attraction device, namely a magnetic induction switch is arranged on the first linear sliding table component, a magnetic induction block is arranged on the specimen holder 102, the magnetic induction switch is turned on to generate magnetism, the magnetic induction block is adsorbed by the magnetic induction switch to grab the specimen holder 102, and the first linear sliding table component slides to drag and/or longitudinally drag the specimen holder 102; the scanning assembly 104 comprises a scanning gun electrically connected with an upper computer, after the specimen rack 102 is dragged out of the first base 101 by the dragging mechanism, the scanning assembly 104 scans information codes on the specimen rack 102 and sends the information acquired by scanning to the upper computer so as to realize information input and management of the experimental specimen.

The automatic transport device 2 as shown in fig. 4 to 6 is used for automatically pushing the reaction tube 9 required by the experiment, and comprises a storage bin plate 201, wherein the storage bin plate 201 is provided with an X-direction guide slot 202 for accommodating the reaction tube 9 and an X-direction pushing mechanism 203 for pushing the reaction tube 9 to slide along the X-direction guide slot 202, the end of the X-direction guide slot 202 is provided with a Y-direction guide slot 204 and a Y-direction pushing mechanism 210 for pushing the reaction tube 9 to slide along the Y-direction guide slot 204, the Y-direction pushing mechanism 210 comprises a second pushing block 205 and an elastic pressing block 206 which can move along the Y-direction and the Z-direction, the second pushing block 205 moves along the Z-direction with the Y-direction pushing mechanism 210 to the side end of the reaction tube 9 and then moves along the Y-direction to push the reaction tube 9, the elastic pressing block 206 is pressed on the upper end of the reaction tube 9, preferably, the X-direction guide slot 202 and the Y-direction guide slot 204 are vertically arranged on the horizontal plane, and the X-direction pushing mechanism 203 comprises a first pushing block 207 accommodated in the X-direction guide slot 202, The first pushing block 207 is driven to slide along the X-direction guide groove 202 by the X-direction lead screw 208 and the X-direction driving member 209, more preferably, at least two X-direction guide grooves 202 and the first pushing block 207 matched with the X-direction guide grooves 202 may be arranged side by side on the storage bin plate 201, and the X-direction pushing mechanism 203 enables the X-direction driving member 209 to simultaneously drive the first pushing blocks 207 in the two X-direction guide grooves 202 to move through the function of the X-direction synchronous belt, so as to improve the working efficiency of the device; the Y-direction guide groove 204 is also provided with a third sensor used for sensing the position of the reaction tube 9, the third sensor is matched with the X-direction driving piece 209, when the third sensor senses the reaction tube 9, namely the reaction tube 9 enters the Y-direction guide groove 204, the third sensor feeds a sensing signal back to the electric control module, the electric control module controls the X-direction driving piece 209 to stop moving, controls the Y-direction pushing mechanism to move, and moves the reaction tube 9 along the Y-direction guide groove 204.

The storage bin plate 201 can be directly and fixedly connected to the rack through the supporting plate 211, the supporting plate 211 can also be connected with the bottom plate 220, and the automatic conveying device 2 is integrally and fixedly arranged on the rack through the bottom plate 220, so that the advantage of modularized assembly is realized, and the installation and maintenance of each mechanism or module are facilitated.

As a further improvement of the above solution, as shown in fig. 7, the Y-direction pushing mechanism 210 includes a first fixing plate 212, a Z-direction driving member 213 for driving the first fixing plate 212 to move along the Z-direction, and a Y-direction driving member 214 for driving the Z-direction driving member 213 to move along the Y-direction, that is, the first fixing plate 212 can move along the Z-direction and the Y-direction, the first fixing plate 212 is provided with a second pushing block 205 fixedly connected by bolts or welding and at least two elastic pressing blocks 206 movably arranged side by side in the vertical direction, a first elastic member 215 is arranged between the elastic pressing blocks 206 and the second pushing block 205, the first elastic member 215 can be a spring, the second pushing block 205 moves downwards along the Z direction along with the first fixing plate 212 to a position between the two reaction tubes 9, at the same time, the elastic pressing block 206 is pressed against the upper end of the reaction tube 9 to confine the reaction tube 9 in the Y-guide groove 204.

As a further improvement of the above scheme, as shown in fig. 5 and 6, a reaction tube rack and a cover rotatably connected to the reaction tube rack are disposed on the storage bin plate 201, the reaction tube rack can accommodate a plurality of reaction tubes 9, in this embodiment, the reaction tube rack can accommodate a plurality of reaction tubes 9, one side of the cover opposite to the reaction tubes 9 is provided with a plurality of first protrusions for crimping the reaction tubes 9, the upper ends of the reaction tubes 9 are crimped by the first protrusions, and then the reaction tubes 9 on the reaction tube rack are crimped into the X-direction guide groove 202, the cover includes a left cover 217 and a right cover 218 hinged to the storage bin plate 201, the storage bin plate 201 is further provided with an electromagnetic lock 219, and the left cover 217 and the right cover 218 are locked to the storage bin plate 201 by the electromagnetic lock 219 in the operation process of the device, so as to prevent accidental operation.

The working principle of the automatic conveying device 2 of the invention is that a reaction tube 9 is loaded on a storage bin plate 201 through a reaction tube rack, a left closing cover 217 and a right closing cover 218 which are hinged on the storage bin plate 201 are closed and pressed on the reaction tube 9, so that the reaction tube 9 falls off from the reaction tube rack into an X-direction guide groove 202, the left closing cover 217 and the right closing cover 218 are locked through an electromagnetic lock 219, the closing covers can not be manually opened when the device works, and misoperation is prevented; the X-direction driving piece 209 drives the first pushing block 207 to push the reaction tube 9 to the Y-direction guide groove 204 along the X-direction guide groove 202, the third sensor senses the reaction tube 9 and then feeds back a sensing signal to the electric control module, the electric control module controls the X-direction driving piece 209 to stop moving and controls the Y-direction pushing mechanism 210 to move, the second pushing block 205 and the elastic pressing block 206 move downwards under the action of the Z-direction driving piece 213, the second pushing block 205 moves to the side surface of the reaction tube 9, the elastic pressing block 206 is in pressure joint with the upper end of the reaction tube 9, and the Y-direction driving piece 214 drives the second pushing block 205 to push the reaction tube 9 out along the Y-direction guide groove 204 to wait for the action of the next process; it should be noted that, automatic conveying device 2 can once only add a plurality of reaction tube 9 through the reaction tube support, and convey reaction tube 9 to specific position, need not artifical manual interpolation one by one, greatly promoted work efficiency, reduce artifical intensity of labour, and can add reaction tube 9 midway at random, make things convenient for humanized experiment operation, during specific operation, can pause automatic conveying device 2 work through electric control module, X resets to pushing mechanism 203 automatically this moment, and electromagnetic lock 219 closes, the lid can be opened and shut to the staff, thereby add reaction tube 9 in the reaction tube support.

As shown in fig. 8, the blending device 3 is used for storing and blending reagents, and includes a loading base 301 having an inner cavity, the bottom of the loading base 301 is provided with a first refrigeration assembly 302, a reagent turntable 306 capable of rotating automatically and a reagent seat 304 eccentrically arranged in the reagent turntable 306 are arranged in the loading base 301, the reagent seat 304 is capable of rotating automatically, specifically, the reagent turntable 306 is connected with a motor through a coupling, which may be a servo motor, and the motor drives the reagent turntable 306 to rotate automatically, so that the reagent seat 304 in the reagent turntable 306 rotates at a certain angle along the axis of the reagent turntable 306; the reagent seat 304 is provided with at least two reagent seats which are eccentrically arranged in the reagent rotating disc 306 along the circumferential direction, in the embodiment, preferably, five reagent seats 304 are uniformly distributed in the reagent rotating disc 306 along the circumferential direction, a connecting seat 309 capable of rotating is arranged in the reagent seat 304, the reagent bottles can be separately arranged on the connecting seat 309 and rotate along with the connecting seat 309, preferably, a reagent bottle sleeve 303 is detachably arranged on the reagent seat 304, and the reagent bottles 305 are accommodated in the reagent bottle sleeve 303, so that the reagent bottles 305 cannot be thrown out of the reagent seats 304 during rotation, and the operation stability of the device is improved; the bottom of the reagent holder 304 is provided with a first transmission assembly for driving the connecting seat 309 to rotate, that is, the connecting seat 309 can rotate with the reagent holder 304 while rotating, the reagent turntable 306 is internally and fixedly provided with a first transmission gear 307, the first transmission assembly comprises a second transmission gear 308 meshed with the first transmission gear 307, the second transmission gear 308 can be eccentrically and rotatably arranged at the bottom of the reagent holder 304, the purpose is that the second transmission gear 308 corresponds to the connecting seat 309 one by one, the eccentric arrangement of the second transmission gear 308 at the bottom of the connecting seat 309 can not only leave a design space, but also ensure that the second transmission gear 308 can be meshed with the first transmission gear 307 to rotate when rotating with the reagent holder 304, specifically, the first transmission gear 307 is an internal gear, the second transmission gear 308 is an external gear, when the second transmission gear 308 rotates with the reagent holder 304, the second transmission gear is meshed with the first transmission gear 307 to rotate the second transmission gear 308, thereby driving the connecting seat 309 to rotate; a connecting shaft and a first limit pin 310 are arranged between the second transmission gear 308 and the connecting seat 309, a first limit baffle matched with the first limit pin 310 is arranged at the bottom of the connecting seat 309, the first limit pin 310 is fixedly connected with the second transmission gear 308 through a first transmission gear 307, when the first limit pin 310 rotates along with the second transmission gear 308, the first limit pin 310 abuts against and pushes the first limit baffle to drive the connecting seat 309 to rotate, preferably, at least two first limit pins 310 are arranged on the left side and the right side of the first limit baffle respectively, so that when the device stops rotating, the connecting seat 309 can be quickly braked through the first limit pin 310, and the first limit pins 310 are arranged on the left side and the right side of the first limit baffle respectively, preferably, the first limit baffle is relatively fixed in the rotating direction relative to the first limit pin 310, so as to improve the stability of the rotation of the connecting seat 309, and prevents the reagent bottle 305 from reversely rotating to cause the reagent in the bottle to be poured out when the reagent seat 304 stops rotating; the reagent holder 304 is connected with a first power member 312, a second transmission assembly is arranged between the reagent holder 304 and the first power member 312, the second transmission assembly comprises a third transmission gear 313 arranged at the output end of the first power member 312 and a fourth transmission gear 314 arranged on the reagent holder 304, the fourth transmission gear 314 corresponds to the reagent holder 304 one by one, the fourth transmission gear 314 is detachably meshed with the third transmission gear 313, when the fourth transmission gear 314 rotates along with the reagent turntable 306 to a position where the third transmission gear 313 is meshed, the fourth transmission gear 314 is driven to rotate by the third transmission gear 313, so that the reagent holder 304 rotates along with the fourth transmission gear 314, and the reagent holder 304 in the reagent turntable 306 is switched to rotate by the rotation of the reagent turntable 306; the blending device 3 further comprises a second fixing plate 315 for fixedly mounting the first power element 312, the second fixing plate 315 is fixedly mounted on the rack in a threaded connection or welding manner, the second fixing plate 315 is further provided with a first sensor for detecting the rotation position of the connecting seat 309, the first sensor is electrically connected with the electrical control module, the first sensor can be a proximity sensor, and preferably, the fourth transmission gear 314 is provided with a first protrusion 317 matched with the first sensor; because connecting seat 309 sets up on reagent seat 304 along circumference branch, when fourth drive gear 314 rotated to first sensor and induced first arch 317, first power piece 312 stopped the drive, then can confirm the position of connecting seat 309 relative reagent seat 304 to the position of reagent bottle 305 can accurately be confirmed to the mechanism of snatching or imbibing of analysis appearance mechanism, assurance device operation's accuracy and stability.

As a further improvement of the above scheme, as shown in fig. 9 and 10, the first refrigeration assembly 302 includes a refrigeration sheet 318, a refrigeration surface of the refrigeration sheet 318 is attached to the bottom of the loading base 301, a heating surface is attached to a water cooling head 319, the water cooling head 319 is connected to a water pump, preferably, the refrigeration sheet 318 and the water cooling head 319 are fixedly connected to the bottom of the loading base 301 through a compression sheet 320 and a screw thread, and the first refrigeration assembly 302 has a working principle that an inner cavity of the loading base 301 is kept at a proper constant temperature by a mechanism of refrigeration on one side of the refrigeration sheet 318 and heat release on the other side, and the heat dissipated by the refrigeration sheet 318 is taken out by the water cooling liquid in the water cooling head 319, so as to ensure the stability of the refrigeration sheet 318 and realize circulating refrigeration; it should be noted that the reagent rotating disk 306 is connected to a motor to realize a rotation function, and preferably, the reagent rotating disk 306 may be connected to a servo motor for switching different blending mechanisms to cooperate with the first power component 312, i.e. switching the third transmission gear 313 of the synchronous reagent holder 304 to mesh with the fourth transmission gear 314.

As shown in fig. 11 to 13, the incubation oscillating device 4 includes a first substrate 401 fixedly mounted on the frame, a heating incubation mechanism and an X-direction shifting mechanism 402 are disposed on the first substrate 401, the heating incubation mechanism includes a heating chamber 403 and a heating film 404 disposed at the bottom of the heating chamber 403, an oscillating mechanism 405 is disposed in the heating chamber 403, the X-direction shifting mechanism 402 is used for driving the oscillating mechanism 405 to move in the heating chamber 403, the heating film 404 includes at least two circles of copper wires circumferentially disposed at the bottom of the heating chamber 403, the two circles of copper wires are divided into an inner circle and an outer circle, because the heat dissipation of the outer circle of the heating chamber 403 is faster, the arrangement density of the outer circle of copper wires can be set to be greater than that of the inner circle of copper wires, such a way can effectively ensure the uniformity of the temperature in the chamber, and the copper wires are divided into a plurality of circles of copper wires, and can be heated individually, so as to achieve the purpose of selective heating, the temperature in the bin can be better adjusted, the energy consumption is reduced, and the heating time is shortened; the X-direction shifting mechanism 402 comprises a third power member 406 and a conveyor belt 407, and the X-direction shifting mechanism 402 drives the oscillating mechanism 405 to move along the X-direction through the conveyor belt 407; the oscillating mechanism 405 comprises an oscillating base block 408 fixedly mounted on a conveyor 407, and further comprises an oscillating lower fixing plate 409, a second elastic member 410 and an oscillating upper fixing plate 411 which are fixedly connected from bottom to top in sequence, the oscillating lower fixing plate 409 is fixed at the upper end of the oscillating base block 408 in a threaded connection manner, the second elastic member 410 can be a rubber column, the bottom surface of the oscillating upper fixing plate 411 is fixedly connected with a fixing seat 412 through a bolt structure, the fixing seat 412 is used for fixedly connecting a rotating source 413, the rotating source 413 is vertically arranged, the output end of the rotating source 413 is accommodated in the area between the fixing seat 412 and the oscillating upper fixing plate 411, the output end of the rotating source 413 is connected with an eccentric member 414, the eccentric member 414 can be an eccentric member, preferably, a groove for accommodating the eccentric member 414 is formed in the fixing seat 412, a connecting block 415 is fixed on the fixing seat 412, and the connecting block 415 vertically penetrates through the oscillating upper fixing plate 411, the connecting block 415 is provided with a first tray 416, the first tray 416 is provided with a plurality of clamping grooves for placing the reaction tubes 9 in a clearance manner, the rotating source 413 drives the eccentric member 414 to rotate, then the first tray 416 is driven to move through the fixing seat 412 and the connecting block 415, and the reaction tubes 9 accommodated on the first tray 416 are driven to form a circumferential vortex oscillation effect under the centrifugal force of the eccentric member 414 and the automatic resetting characteristic of the second elastic member 410.

Preferably, the connection block 415 of the oscillating mechanism 405 passes through the oscillating upper fixing plate 411 and the heating chamber 403 in sequence, so that the first tray 416 can be accommodated in the heating chamber 403, and the heating film 404 is disposed at the bottom of the heating chamber 403, that is, the heating film 404 is disposed at a position between the oscillating upper fixing plate 411 and the first tray 416, preferably, a waist-shaped hole for allowing the connection block 415 to pass through is disposed on the heating film 404, and the connection block 415 can oscillate horizontally along a direction perpendicular to the waist-shaped hole, for the purpose that the oscillating motion refers to a centrifugal rotating motion along the circumferential direction horizontally, and therefore, the hole width of the waist-shaped hole is sized to ensure that the connection block 415 can perform the centrifugal rotating motion on the horizontal plane.

The realization principle of this embodiment is, place reaction tube 9 through the first tray 416 of holding in the storehouse 403 that generates heat, generate heat in the storehouse 403 because of the effect of heating film 404 produces homothermal effect, the rotation source 413 that first tray 416 is connected rotates simultaneously, it rotates to drive eccentric 414 to do the centrifugation, then drive fixing base 412, rotation source 413, connecting block 415 and first tray 416 all are oscillatory motion, its specific combined action of restoring force with second elastic component 410, make reaction tube 9 reach the effect of swirl oscillation, make experiment sample and reagent in reaction tube 9 can fully mix, through X to displacement mechanism 402 drive oscillating mechanism 405 removal forward continuous transport reaction tube 9, reaction tube 9 can also incubate the oscillation when continuously conveying forward promptly, work efficiency is promoted.

In this embodiment, the rotating source 413 and the third power member 406 may be motors, and the third power member 406 is preferably a servo motor.

As shown in fig. 14, the washing mechanism 5 includes a second substrate 501 fixed on the rack, and a liquid distribution assembly disposed on the second substrate 501, the liquid distribution assembly includes a liquid injection peristaltic pump 502 and a liquid suction peristaltic pump 503, preferably, three liquid injection peristaltic pumps 502 and one liquid suction peristaltic pump 503 are adopted in this embodiment, the liquid injection peristaltic pumps 502 and the peristaltic pump 503 are respectively connected with a cleaning needle 504 in a pipe, the liquid injection peristaltic pumps 502 are used for respectively injecting the experimental sample, the diluent and the reagent into the reaction tube 9, and the liquid suction peristaltic pump 503 is used for sucking away the waste liquid or the cleaning liquid in the reaction tube 9; the second base plate 501 is further provided with a first motion assembly 505 for driving the cleaning needle 504 to move along the transverse direction and the vertical direction, and the first motion assembly 505 can be a combination of a transverse linear sliding table 803 and a vertical linear sliding table commonly used in the market.

As a further improvement of the above scheme, the washing mechanism 5 further includes a magnetic plate assembly 506, the magnetic plate assembly 506 includes a third fixing plate with a chute 105 and magnets respectively disposed at two sides of the chute 105, the reaction tube 9 is conveyed into the chute 105 of the magnetic plate assembly 506, the magnets at two sides can adsorb the magnetic beads or magnetic sheets of the experimental samples attached to the reaction tube 9, thereby facilitating the washing operation of the reaction tube 9, and ensuring that the magnetic beads or magnetic sheets are not lost in the washing process to affect the experimental results.

The interpretation module 6 can be understood as an optical detection system commonly used in the market, and comprises a platform for accommodating the reaction tube 9, a light source emitting assembly arranged at the bottom of the platform and a light receiver arranged at the top of the platform, namely, light is transmitted from top to bottom and passes through the reaction tube 9 and is received by the backlight receiver behind the magnetic sheet or the magnetic bead in the reaction tube 9, so that the magnetic sheet or the magnetic bead is optically detected, and the interpretation of the experimental result is realized.

As shown in fig. 15 and 17, the magnetic sheet tiling apparatus 7 includes a third base plate 701, vertical plates 702, a fourth fixing plate 703, a second base 704 and a second tray 705, which are sequentially disposed from bottom to top, in this embodiment, the multiple immunoassay analyzer preferably uses a reagent containing magnetic sheets, the third base plate 701 is fixedly connected to the rack, the vertical plates 702 are respectively disposed on the third base plate 701 by bolts or welding, the fourth fixing plate 703 is disposed at a position between the two vertical plates 702, the fourth fixing plate 703 is used for fixedly mounting a fourth power member 706, the second base 704 is fixedly mounted at the top ends of the vertical plates 702, stated another way, the second base 704 is disposed in an area between the fourth fixing plate 703 and the second tray 705, an output end of the fourth power member 706 is connected to an eccentric rotating shaft 707, the eccentric rotating shaft 707 is connected to the second tray 705, the fourth power member 706 drives the eccentric rotating shaft 707 to rotate, the second tray 705 is driven to swing along the circumferential direction, a clamping groove 717 matched with the reaction tube rack is formed in the second tray 705, the reaction tube 9 is arranged on the reaction tube rack, the reaction tube rack and the second tray 705 are detachably connected, namely the reaction tube rack is clamped on the second tray 705 from the clamping groove 717, so that the reaction tube 9 can swing along the circumferential direction along with the second tray 705 to achieve the functions of uniformly mixing magnetic sheets and flatly spreading the magnetic sheets at the bottom of the reaction tube 9.

As a further improvement of the above solution, as shown in fig. 15 and 16, a plurality of reset components 708 are disposed between the second base 704 and the second tray 705, in this embodiment, preferably, the second base 704 is square, the reset components 708 are disposed at four corners of the second base 704, specifically, reset grooves for accommodating the reset components 708 are disposed at four corners of the second base 704, the reset components 708 include a reset sleeve 709 accommodated in the reset grooves, a reset rod 710 sleeved in the reset sleeve 709, and a third elastic member 711 disposed in an area between the reset sleeve 709 and the reset grooves, the reset rod 710 and the reset sleeve 709 are in interference fit connection, the third elastic member 711 may be a compression spring, the tray is pressed against the top of the reset rod 710 due to the self gravity, the upper reset rod 710 moves vertically downward to be pressed against the third elastic member 711, the third elastic member 711 is deformed by compression, its aim at makes second tray 705 can with second base 704 flexonics, guarantees that second tray 705 smoothly swings along circumference, realizes the function of magnetic sheet mixing and tiling in reaction tube 9 bottom.

As a further improvement of the above scheme, as shown in fig. 15, the eccentric rotating shaft 707 may be connected to an output end of the fourth power member 706 through a coupling or an interference fit, the fourth power member 706 preferably employs a servo motor, and a counterweight 712 is disposed on the eccentric rotating shaft 707, so as to increase a centrifugal force of the eccentric rotating shaft 707 and enhance a circumferential swinging effect of the second tray 705; a bearing 713 and a second limit pin 714 for fixedly connecting the eccentric rotating shaft 707 and the bearing 713 are arranged between the second tray 705 and the eccentric rotating shaft 707, specifically, a groove for accommodating the bearing 713 is arranged at the bottom of the second tray 705, the upper end of the eccentric rotating shaft 707 is sleeved on the inner ring of the bearing 713, the bearing 713 is fixedly connected with the second tray 705 in an interference manner, and the upper end of the eccentric rotating shaft 707 prevents the bearing 713 and the second tray 705 from coming off through the second limit pin 714; preferably, the bottom of the second tray 705 is further provided with a limiting hole for receiving the upper end of the reset rod 710, which aims to ensure the swing amplitude of the second tray 705 through the cooperation of the reset rod 710 and the limiting hole, and to realize the positioning or resetting of the second tray 705 through the cooperation of the four reset rods 710 and the reset holes when the device stops operating.

As a further improvement of the above solution, as shown in fig. 15, a second sensor 715 for detecting the rotation position of the reaction tube 9 is disposed on the fourth fixing plate 703, and a sensing second protrusion 716 matched with the second sensor 715 is disposed on the sidewall of the counterweight 712, preferably, the second sensor 715 may be a photoelectric sensor, since the reaction tube loading cassette is matched with the second tray 705 in a clamping manner, the matching angle is fixed, and the swing direction of the second tray 705 can be determined by detecting the rotation position of the eccentric rotating shaft 707, so as to detect the position of the reaction tube 9.

The transfer mechanism 8 shown in fig. 18 comprises a sample adding assembly, a gripping assembly 807, a first guide rail 805 and a second guide rail 806 which are arranged in parallel along the X direction, respectively, wherein the sample adding assembly can comprise a transverse linear sliding table 803, a longitudinal linear sliding table 804 and a liquid transferring needle 802, the liquid transferring needle 802 can be driven by the combination of the transverse linear sliding table 803 and the longitudinal linear sliding table 804 to move along the X direction and the Z direction to transfer liquid, the sample adding assembly is divided into a first sample adding assembly for adding an experimental sample and a diluent, a second sample adding assembly for adding a reagent and a third sample adding assembly for adding a detection liquid, the first sample adding assembly and the second sample adding assembly are respectively arranged on the first guide rail 805 and can respectively move along the first guide rail 805, the third sample adding assembly and the gripping assembly 807 are respectively arranged on the second guide rail 806 and can move along the second guide rail 806, preferably, the third sample adding assembly further comprises a rotary air cylinder, the purpose is to realize the rotation of the detection liquid transferring needle 802 and further improve the liquid transferring precision of the detection liquid; the gripping assembly 807 comprises at least two clamping jaws, a clamping cylinder for driving the clamping jaws to move relatively close to and away from each other, and a third movement assembly for driving the clamping jaws to move longitudinally along the second guide rail 806, and the third movement assembly can also be a combination of two linear sliding tables; the immunoassay analyzer of this embodiment, it realizes the interpolation of experiment sample and diluent through first application of sample subassembly, realizes the interpolation of reagent through second application of sample subassembly, realizes transferring of detection liquid through third application of sample subassembly to realize transferring reaction tube 9 through tongs subassembly 807.

A card pushing mechanism 10 shown in fig. 19 for transferring the reaction tube 9 from the previous functional position to the next functional position, i.e. the card pushing mechanism 10 is used for transferring the reaction tube 9; the card pushing mechanism 10 includes a card pushing assembly and a second moving assembly for driving the card pushing assembly to move along the transverse direction and the longitudinal direction, the card pushing assembly includes a push plate 1003, a plurality of clamping positions 1004 arranged on the push plate 1003 and used for pushing the reaction tubes 9 to move, and a press rod 1005 arranged in the clamping positions 1004, the press rod 1005 is sleeved with a fourth elastic member 1006, the fourth elastic member 1006 can be a pressure spring, and when the second moving assembly can drive the card pushing assembly to move down along the longitudinal direction, the press rod 1005 is pressed and connected with the upper ends of the reaction tubes 9 to prevent the reaction tubes 9 from being separated from the working positions; the second moving assembly may include a combination of a motor and a belt, which are commonly used in the market, that is, the second moving assembly may be divided into a first motor and belt assembly 1007 for driving the card pushing assembly to move in the transverse direction and a second motor and belt assembly 1008 for driving the card pushing assembly to move in the longitudinal direction.

As shown in fig. 20, the second pick-and-place mechanism 11 is configured to store a specimen diluent, the second pick-and-place mechanism 11 includes a fourth substrate 1101 fixed on the rack, a washing tank 1102 and a diluent bottle holder 1103 that are respectively disposed on the fourth substrate 1101, a second refrigeration assembly 1104 is disposed at the bottom of the diluent bottle holder 1103, the second refrigeration assembly 1104 includes a refrigeration sheet 318, and after a specimen reagent is added to the first sample adding assembly of the transfer mechanism 8, the first sample adding assembly moves the liquid transfer needle 802 to the washing tank 1102 to clean the first sample adding assembly, sucks the diluent, transfers the diluent to the reaction tube 9, and dilutes the experimental specimen to a certain multiple.

As shown in fig. 21 to 23, the reaction tube 9 includes a support 901, a reaction tank 902 disposed on the support 901, and dilution tanks 903 respectively disposed at the left and right sides of the reaction tank 902, which are used for conveniently diluting the experimental specimen to different times, so as to perform multiple immunoreactions in sequence, wherein the reaction tank 902 is made of an optical plastic material, which may be PMMA, PS, PC, TPX, or the like, and has high light transmittance, which can improve the accuracy of the interpretation result; preferably, the height of the bottom of the reaction tank 902 relative to the upper end face of the support 901 is lower than the height of the bottom of the dilution tank 903 relative to the upper end face of the support 901, that is, the reaction tube 9 is placed on a horizontal plane, and the bottom of the reaction tank 902 is not in contact with the horizontal plane, so as to prevent the bottom of the reaction tank 902 from scratching and affecting the interpretation result; preferably, the upper ends of the reaction tank 902 and the dilution tank 903 are both provided with a convex brim 904 along the circumferential direction, which is used for preventing liquid possibly remaining on the surface of the reaction tube 9 from flowing into the reaction tank 902 or the dilution tank 903 to cause pollution; preferably, the left side and the right side of the support 901 are respectively provided with a gripper position 905, the gripper positions 905 are arranged in an arc shape, one side of the gripper position 905 away from the support 901 is provided with a groove, and the structure of the gripper position 905 is matched with the clamping jaw of the gripper assembly 807, so that the stability of the reaction tube 9 in the transferring process is ensured, and the reaction tube 9 is prevented from falling; secondly, the hand holding position 905 is matched with the second pushing block 205 and the blocking position 1004, so that the second pushing block 205 and the blocking position 1004 can conveniently drive the reaction tube 9 to move, and the moving accuracy of the reaction tube 9 is improved; preferably, the lower ends of the left and right sides of the support 901 are provided with guide grooves 907, the guide grooves 907 are vertically recessed upwards, the guide grooves 907 are matched with the X-direction guide groove 202 and the Y-direction guide groove 204 of the automatic conveying device 2, so that the reaction tubes 9 can be conveniently transferred in the automatic conveying device 2, and the transferring accuracy of the reaction tubes 9 is improved; preferably, the reaction tube 9 of the present invention adopts a symmetrical structure, which aims to reduce the production cost and enable the robot arm structure to flexibly grip the reaction tube 9.

The specific use is that after the experimental sample is scanned and information is recorded, the experimental sample is added into two dilution tanks 903 of the reaction tube 9 through a first sample adding component, after the first sample adding component is cleaned by the cleaning tank 1102, the dilution liquid is injected into the two dilution tanks 903 according to different proportions or components, so that the experimental sample has different dilution times, injecting one of the diluted experimental samples into the reaction tank 902 through the third sample adding assembly, injecting a reagent into the reaction tank 902 through the second sample adding assembly, incubating the reaction tube 9 through oscillation of the incubation oscillation mechanism 405 to enable the experimental sample to fully react with the reagent, ensuring the accuracy of an experimental result, cleaning the reacted solution in the reaction tank 902 through the washing mechanism 5, and adsorbing magnetic sheets or magnetic beads on the inner wall of the reaction tank 902 through the magnetic sheet assembly 506 of the washing mechanism 5 to prevent the magnetic sheets or magnetic beads from being cleaned when the reaction tank 902 is cleaned; and injecting another dilution multiple of the experimental sample into the reaction tank 902, and repeating the reagent adding and oscillation incubation operations to realize multiple immunoreactions and improve the working efficiency.

The electric control module can be a device which is commonly used in the market and is used for driving a device or automatically operating a mechanism, and can comprise a programmable controller or a PLC or a single chip microcomputer, a motor or a motor, a pump and/or a valve, an electric connecting wire and the like.

The above is only a preferred embodiment of the present invention, and the present invention is not limited to the above embodiments, and the present invention shall fall within the protection scope of the present invention as long as the technical effects of the present invention are achieved by the same means.

Claims

1. The utility model provides a multiple immunoassay appearance, including the frame, its characterized in that still includes:

a first pick-and-place mechanism (1) which can pick and place an experimental sample;

an automatic transfer device (2) capable of transferring the reaction tube (9);

a mixing device (3) capable of mixing the reagent;

the incubation oscillation device (4) can oscillate and uniformly mix the experimental sample and the reagent and provide the temperature required by the reaction of the experimental sample and the reagent;

a transfer mechanism (8) capable of transferring the reagent and the detection liquid;

a second pick-and-place mechanism (11) which can pick and place the diluent;

the blending device (3) comprises a rotatable reagent turntable (306) and a reagent seat (304) eccentrically arranged on the reagent turntable (306), the reagent seat (304) can rotate, a rotatable connecting seat (309) is arranged in the reagent seat (304), and the connecting seat (309) is eccentrically arranged on the reagent seat (304);

a first transmission gear (307) is fixedly installed in the reagent rotating disc (306), a second transmission gear (308) meshed with the first transmission gear (307) is connected to the connecting base (309), and when the connecting base (309) rotates along with the reagent base (304), the second transmission gear (308) is meshed with the first transmission gear (307) to rotate, so that the connecting base (309) is driven to rotate;

the first transmission gear (307) is an internal gear, and the second transmission gear (308) is an external gear;

the reagent seat (304) is connected with a power piece (312), a second transmission assembly is arranged between the reagent seat (304) and the power piece (312), and the second transmission assembly comprises a third transmission gear (313) arranged at the output end of the power piece (312) and a fourth transmission gear (314) arranged on the reagent seat (304);

the fourth transmission gears (314) correspond to the reagent seats (304) one by one, and the fourth transmission gears (314) are detachably meshed with the third transmission gears (313).

2. The multiple immunoassay analyzer of claim 1, wherein the automatic transfer device (2) comprises an X-direction pushing mechanism (203) for pushing the reaction tube (9) to move in the X-direction, and a Y-direction pushing mechanism (210) for pushing the reaction tube (9) to move in the Y-direction.

3. The multiple immunoassay analyzer of claim 2, wherein the Y-direction pushing mechanism (210) comprises a second pushing block (205) capable of moving along the Y-direction and the Z-direction, and the second pushing block (205) is provided with an elastic pressing block (206).

4. The multiple immunoassay analyzer of claim 1, wherein the incubation oscillating device comprises a rotation source (413), an eccentric member (414) disposed at an output end of the rotation source (413), and a first tray (416) disposed on the rotation source (413), wherein the rotation source (413) drives the eccentric member (414) to rotate, and the first tray (416) oscillates with the eccentric member (414) along a circumferential direction.

5. The multiple immunoassay analyzer of claim 4, wherein the incubation oscillation device further comprises a heat-generating incubation mechanism capable of accommodating the first tray (416), and an X-direction displacement mechanism (402) for driving the first tray (416) to move.

6. The multiple immunoassay analyzer of claim 1, further comprising a magnetic sheet spreading means (7) capable of spreading the magnetic sheet in the detection solution on the bottom of the reaction tube (9).

7. The multiple immunoassay analyzer of claim 6, wherein the magnetic sheet laying device (7) comprises an eccentric rotating shaft (707) and a second tray (705) disposed on the upper portion of the eccentric rotating shaft (707), and the eccentric rotating shaft (707) drives the second tray (705) to swing along the circumferential direction.

8. The multiple immunoassay analyzer of claim 7, wherein the magnetic sheet tiling means (7) further comprises a second base (704) fixedly installed and a reset assembly (708) disposed on the second base (704), wherein an upper end of the reset assembly (708) abuts against the second tray (705).