CN109090163A - A kind of bacteriostatic agent and preparation method thereof of pure plant formula - Google Patents
A kind of bacteriostatic agent and preparation method thereof of pure plant formula Download PDFInfo
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- CN109090163A CN109090163A CN201810756882.2A CN201810756882A CN109090163A CN 109090163 A CN109090163 A CN 109090163A CN 201810756882 A CN201810756882 A CN 201810756882A CN 109090163 A CN109090163 A CN 109090163A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
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Abstract
The invention discloses a kind of bacteriostatic agents of pure plant formula, including below according to the raw material of parts by weight: 35 parts of leaf of Chinese ilex, 28 parts of leek, 22 parts of dried peppermint leaf, 13 parts of pithecellobium clypearia, 11 parts of kuh-seng, 3 parts of solubilizer.The present invention is combined using various plants formula, is used securely and reliably, and the weaker defect of existing bacteriostatic agent broad-spectrum antibacterial effect can be effectively overcomed under the synergistic effect of each component, sphere of action is wider, and effect is more preferable.
Description
Technical field
The present invention relates to a kind of bacteriostatic agent, the bacteriostatic agent and preparation method thereof of specifically a kind of pure plant formula.
Background technique
Around germ, at every moment encroaching on our body, most of the time, our intracorporal immune systems,
The invasion of these germs can be dealt with.But if the unexpected mass propagation of germ has been more than the range that system of defense can be dealt with,
We will be sick.Therefore, from the perspective of with regard to preventive medicine, it is critically important for inhibiting the growth of bacterium.
Extending daily necessities storage life, during guaranteeing daily necessities quality, daily necessities bacteriostatic agent has very important
Effect.The main component of traditional daily necessities bacteriostatic agent is synthetic, and this synthesis bacteriostatic agent has toxicity,
Many countries are used by gradually limitation, therefore daily necessities additive has been formed toward nontoxic, safety, what natural direction was developed
International trend.
Existing plant bacteriostatic agent mostly using single plant as raw material, although to a few class daily necessities harmful bacteria inhibitory effects compared with
It is good, but broad-spectrum antibacterial effect is weaker, and overall performance is not satisfactory.
Summary of the invention
It is mentioned above in the background art to solve the purpose of the present invention is to provide a kind of bacteriostatic agent of pure plant formula
Problem.
To achieve the above object, the invention provides the following technical scheme:
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 35-46 parts of leaf of Chinese ilex, leek 28-
36 parts, 22-31 parts of dried peppermint leaf, 13-21 parts of pithecellobium clypearia, 11-19 parts of kuh-seng, 3-7 parts of solubilizer.
As a further solution of the present invention: including below according to the raw material of parts by weight: 38-43 parts of leaf of Chinese ilex, leek 30-
34 parts, 24-29 parts of dried peppermint leaf, 15-19 parts of pithecellobium clypearia, 13-17 parts of kuh-seng, 4-6 parts of solubilizer.
As further scheme of the invention: including below according to the raw material of parts by weight: 41 parts of leaf of Chinese ilex, leek 32
Part, 26 parts of dried peppermint leaf, 17 parts of pithecellobium clypearia, 15 parts of kuh-seng, 5 parts of solubilizer.
As the present invention further scheme: the solubilizer by a kind of in polyethylene glycol 400, Macrogol 600 or
Two kinds of person are composed.
As the present invention further scheme: the solubilizer by polyethylene glycol 400 and Macrogol 600 by weight
1:2 is mixed.
The preparation method of the bacteriostatic agent of the pure plant formula, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf being crushed, the dehydrated alcohol that 8-11 times of quality times of the two is added extracts twice, each
Extraction temperature is 65-78 DEG C, extraction time 1.3-1.5h, merges extracting solution twice, is concentrated into the 15-20% of original volume, is made
Obtain the first extracting solution;
2) crush, sieve with 100 mesh sieve after dehydrating leek, be added 7-9 times of quality times distillation flooding three times, every time
Extraction temperature be 60-72 DEG C.Extraction time is 2.2-2.6h, merges extracting solution three times, is concentrated into the 20-25% of original volume,
Leek extracts are made;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1-1.2h is carried out at 62-70 DEG C, is during which added thereto solubilizer, must mix after reaction
Liquid;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 40-55% of original volume to get the bacteriostatic agent.
As further scheme of the invention: ultrasonic power is 1000W in the step 4).
Compared with prior art, the beneficial effects of the present invention are:
The present invention is combined using various plants formula, is used securely and reliably, and in the synergistic effect of each component
The weaker defect of existing bacteriostatic agent broad-spectrum antibacterial effect can be effectively overcomed down, and sphere of action is wider, and effect is more preferable.
Specific embodiment
The technical solution of the patent is explained in further detail With reference to embodiment.
Embodiment 1
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 35 parts of leaf of Chinese ilex, 28 parts of leek,
22 parts of dried peppermint leaf, 13 parts of pithecellobium clypearia, 11 parts of kuh-seng, 3 parts of solubilizer;
Wherein the solubilizer is polyethylene glycol 400.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 8 times of the two quality times is added extracts each extraction twice
Temperature is 65 DEG C, extraction time 1.3h, merges extracting solution twice, is concentrated into the 15% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 7 times of distillation flooding is added three times, each
Extraction temperature is 60 DEG C.Extraction time is 2.2h, merges extracting solution three times, is concentrated into the 20% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1h is carried out at 62 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 40% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Embodiment 2
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 38 parts of leaf of Chinese ilex, 30 parts of leek,
24 parts of dried peppermint leaf, 15 parts of pithecellobium clypearia, 13 parts of kuh-seng, 4 parts of solubilizer;
Wherein the solubilizer is Macrogol 600.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 9 times of the two quality times is added extracts each extraction twice
Temperature is 70 DEG C, extraction time 1.4h, merges extracting solution twice, is concentrated into the 20% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 8 times of distillation flooding is added three times, each
Extraction temperature is 62 DEG C.Extraction time is 2.4h, merges extracting solution three times, is concentrated into the 20% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1h is carried out at 66 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 45% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Embodiment 3
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 41 parts of leaf of Chinese ilex, 32 parts of leek,
26 parts of dried peppermint leaf, 17 parts of pithecellobium clypearia, 15 parts of kuh-seng, 5 parts of solubilizer;
Wherein the solubilizer is mixed by polyethylene glycol 400 and Macrogol 600 by weight 1:2.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 10 times of the two quality times is added extracts each leaching twice
Temperature raising degree is 70 DEG C, extraction time 1.5h, merges extracting solution twice, is concentrated into the 15% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 8 times of distillation flooding is added three times, each
Extraction temperature is 65 DEG C.Extraction time is 2.4h, merges extracting solution three times, is concentrated into the 20% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1h is carried out at 65 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 50% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Embodiment 4
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 39 parts of leaf of Chinese ilex, 31 parts of leek,
25 parts of dried peppermint leaf, 18 parts of pithecellobium clypearia, 15 parts of kuh-seng, 6 parts of solubilizer;
Wherein the solubilizer is Macrogol 600.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 9 times of the two quality times is added extracts each extraction twice
Temperature is 68 DEG C, extraction time 1.4h, merges extracting solution twice, is concentrated into the 15% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 8 times of distillation flooding is added three times, each
Extraction temperature is 65 DEG C.Extraction time is 2.3h, merges extracting solution three times, is concentrated into the 20% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1.1h is carried out at 62 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 45% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Embodiment 5
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 43 parts of leaf of Chinese ilex, 34 parts of leek,
29 parts of dried peppermint leaf, 19 parts of pithecellobium clypearia, 17 parts of kuh-seng, 6 parts of solubilizer;
Wherein the solubilizer is polyethylene glycol 400.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 11 times of the two quality times is added extracts each leaching twice
Temperature raising degree is 70 DEG C, extraction time 1.4h, merges extracting solution twice, is concentrated into the 15% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 7 times of distillation flooding is added three times, each
Extraction temperature is 65 DEG C.Extraction time is 2.5h, merges extracting solution three times, is concentrated into the 20% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1h is carried out at 65 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 45% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Embodiment 6
A kind of bacteriostatic agent of pure plant formula, including below according to the raw material of parts by weight: 46 parts of leaf of Chinese ilex, 36 parts of leek,
31 parts of dried peppermint leaf, 21 parts of pithecellobium clypearia, 19 parts of kuh-seng, 7 parts of solubilizer;
Wherein the solubilizer is polyethylene glycol 400.
The preparation method of the bacteriostatic agent of pure plant formula in the present embodiment, comprising the following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 11 times of the two quality times is added extracts each leaching twice
Temperature raising degree is 78 DEG C, extraction time 1.5h, merges extracting solution twice, is concentrated into the 20% of original volume, and the first extracting solution is made;
2) it crushes, sieves with 100 mesh sieve after dehydrating leek, quality times 9 times of distillation flooding is added three times, each
Extraction temperature is 72 DEG C.Extraction time is 2.6h, merges extracting solution three times, is concentrated into the 25% of original volume, and leek is made and extracts
Liquid;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonic wave
In processor, ultrasonic treatment 1.2h is carried out at 70 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 55% of original volume to get the bacteriostatic agent.
Ultrasonic power is 1000W in the step 4).
Comparative example 1: in addition to without leaf of Chinese ilex, formula is consistent with preparation process and embodiment 3.
Comparative example 2: in addition to without pithecellobium clypearia, formula is consistent with preparation process and embodiment 3.
Comparative example 3: containing only leaf of Chinese ilex and pithecellobium clypearia, and preparation process and embodiment 3 are consistent.
Performance test
Bacteriostatic agent corresponding to Example 3 and comparative example 1-3 carries out antibacterial comparative test under the same conditions, takes examination
Tube method is tested, and is carried out minimal inhibitory concentration measurement (MIC), obtains following table after recording data
Data corresponding to embodiment 3 and comparative example 1-3 can be seen that the present invention and make in the collaboration of each component from upper table
Under, have biggish antimicrobial spectrum, effectively overcome the weaker defect of existing bacteriostatic agent broad-spectrum antibacterial effect, acts on model
It encloses more extensively, effect is more preferable.
It is obvious to a person skilled in the art that invention is not limited to the details of the above exemplary embodiments, Er Qie
In the case where without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, the present embodiments are to be considered as illustrative and not restrictive, and the scope of the present invention is by appended power
Benefit requires rather than above description limits, it is intended that all by what is fallen within the meaning and scope of the equivalent elements of the claims
Variation is included within the present invention.
In addition, it should be understood that although this specification is described in terms of embodiments, but not each embodiment is only wrapped
Containing an independent technical solution, this description of the specification is merely for the sake of clarity, and those skilled in the art should
It considers the specification as a whole, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
The other embodiments being understood that.
Claims (7)
1. a kind of bacteriostatic agent of pure plant formula, which is characterized in that including below according to the raw material of parts by weight: leaf of Chinese ilex 35-46
Part, 28-36 parts of leek, 22-31 parts of dried peppermint leaf, 13-21 parts of pithecellobium clypearia, 11-19 parts of kuh-seng, 3-7 parts of solubilizer.
2. the bacteriostatic agent of pure plant formula according to claim 1, which is characterized in that including below according to the original of parts by weight
Material: 38-43 parts of leaf of Chinese ilex, 30-34 parts of leek, 24-29 parts of dried peppermint leaf, 15-19 parts of pithecellobium clypearia, 13-17 parts of kuh-seng, solubilizer 4-
6 parts.
3. the bacteriostatic agent of pure plant formula according to claim 2, which is characterized in that including below according to the original of parts by weight
Material: 41 parts of leaf of Chinese ilex, 32 parts of leek, 26 parts of dried peppermint leaf, 17 parts of pithecellobium clypearia, 15 parts of kuh-seng, 5 parts of solubilizer.
4. the bacteriostatic agent of pure plant formula according to claim 3, which is characterized in that the solubilizer is by polyethylene glycol
400, one or two kinds of in Macrogol 600 to be composed.
5. the bacteriostatic agent of pure plant formula according to claim 3, which is characterized in that the solubilizer is by polyethylene glycol
400 and Macrogol 600 mixed by weight 1:2.
6. a kind of preparation method of the bacteriostatic agent of pure plant formula a method as claimed in any one of claims 1 to 5, which is characterized in that including
Following steps:
1) leaf of Chinese ilex and dried peppermint leaf are crushed, the dehydrated alcohol that 8-11 times of quality times of the two is added extracts each extraction twice
Temperature is 65-78 DEG C, extraction time 1.3-1.5h, merges extracting solution twice, is concentrated into the 15-20% of original volume, is made the
One extracting solution;
2) crush, sieve with 100 mesh sieve after dehydrating leek, be added 7-9 times of quality times distillation flooding three times, each leaching
Temperature raising degree is 60-72 DEG C.Extraction time is 2.2-2.6h, merges extracting solution three times, is concentrated into the 20-25% of original volume, is made
Leek extracts;
3) it will be mixed after pithecellobium clypearia drying with kuh-seng, crush, cross 150 meshes, obtain mixed-powder;
4) merge the first extracting solution and leek extracts, mixed-powder is added and is thoroughly mixed, is subsequently placed in ultrasonication
In device, ultrasonic treatment 1-1.2h is carried out at 62-70 DEG C, is during which added thereto solubilizer, obtain mixed liquor after reaction;
5) mixed liquor is filtered by vacuum, concentrates the filtrate to the 40-55% of original volume to get the bacteriostatic agent.
7. the preparation method of the bacteriostatic agent of pure plant formula according to claim 6, which is characterized in that in the step 4)
Ultrasonic power is 1000W.
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Cited By (1)
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CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
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CN108159108A (en) * | 2018-02-26 | 2018-06-15 | 辽宁昱众合医药科技有限公司 | The preparation of pithecellobium clypearia total phenolics and its application in antimicrobial antiphlogistic drug |
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CN101637189A (en) * | 2009-08-21 | 2010-02-03 | 安徽神健生物工程有限公司 | Compound traditional Chinese medicine disinfection solution and preparation method thereof |
CN103349025A (en) * | 2013-07-17 | 2013-10-16 | 黄利辉 | Botanical disinfecting, sterilizing and deodorizing liquid, and preparation method and application thereof |
CN107788229A (en) * | 2017-07-27 | 2018-03-13 | 浦江县合洪园艺研发有限公司 | A kind of purposes of plant-based bacteriostat |
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CN113349227A (en) * | 2021-05-12 | 2021-09-07 | 湖南省烟草公司株洲市公司 | Tobacco seed germination-promoting stress-resistant seedling-strengthening composition, preparation and use method |
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