CN106491411A - One kind is rich in natural Flos Matricariae chamomillae petal and its hydrosol - Google Patents
One kind is rich in natural Flos Matricariae chamomillae petal and its hydrosol Download PDFInfo
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- CN106491411A CN106491411A CN201610887135.3A CN201610887135A CN106491411A CN 106491411 A CN106491411 A CN 106491411A CN 201610887135 A CN201610887135 A CN 201610887135A CN 106491411 A CN106491411 A CN 106491411A
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- picked
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- matricariae chamomillae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/4973—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom
- A61K8/498—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with oxygen as the only hetero atom having 6-membered rings or their condensed derivatives, e.g. coumarin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/005—Preparations for sensitive skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/10—Washing or bathing preparations
Abstract
The invention discloses a kind of natural Flos Matricariae chamomillae petal and its hydrosol rich in picked by hand, the main natural Flos Matricariae chamomillae petal for including picked by hand, Flos Matricariae chamomillae flavone etc..Moisturizing, antibacterial, improve allergy skin, delay skin aging.
Description
Technical field
The present invention relates to daily necessities field, specially one kind is rich in natural Flos Matricariae chamomillae petal and its hydrosol.
Background technology
Bath gel be a kind of formed by surfactant and conditioner modulation, usual only clean result, single function,
With the raising of human living standard, effect that bath gel is had also constantly is increasing, such as antibacterial, anti-ageing work(of waiting for a long time
Effect is increasingly welcome by consumers in general.《A kind of Value of natural bath ＆ shower dew for baby》(application number：CN201210456051.6),
Disclose a kind of containing Semen Coicis, Paeonia suffruticosa extracting solution, Aloe juice, Olive oil quintessence, Poria, Cortex Phellodendri, natural propolis, vitamin, plant
The bath gel of the compositions such as Flos Matricariae chamomillae, cleanable skin, anti-inflammation, but it has been short of in terms of antioxidation；《A kind of natural beauty
White bath gel》(application number：CN201210316043.1), disclose one kind and contain Flos Matricariae chamomillae, Herba Dracocephali, Lac Bovis seu Bubali, Cortex cocois radiciss oils and fatss
Fat acid diglycollic amide, the bath gel of the composition such as almond oil, strong detergency, property are gentle, have no stimulation, moist skin, strong
Body disappears fatigue, in terms of being equally not directed to antioxidation, and uses Flos Matricariae chamomillae herb, and effect is not notable.
Content of the invention
For making up problems and shortcomings present in existing bath gel, the invention discloses a kind of rich in the natural of picked by hand
Flos Matricariae chamomillae petal and its hydrosol.Fragrant odour, relieving mood, foaming abundancy are easily rinsed, moderate performance, with moisturizing, antibacterial
While function, the free radical of skin surface can also be removed, and delay skin aging makes skin recover delicate.
The present invention is achieved by the following scheme：
A kind of natural Flos Matricariae chamomillae petal and its hydrosol rich in picked by hand, mainly includes following component：
| Component | Weight portion/part |
| Natural Flos Matricariae chamomillae petal | 2-5 |
| Flos Matricariae chamomillae flavone | 0.02-0.08 |
| Surfactant | 11.5-25 |
| Preservative | 0.015-0.048 |
| Chelating agen | 0.1-0.2 |
| PH regulators | 0.05-0.1 |
| Dipropylene glycol | 5-10 |
| Polyethylene Glycol -90M | 0.2-1.5 |
| UVNUL MS-40 | 0.02-0.07 |
| Glycine | 0.05-0.1 |
| Essence | 0.1-0.2 |
| Pigment | 0.05-0.1 |
| Deionized water | 48-85 |
Further, the surfactant is sodium laureth sulfate, cocamido propyl betaine, lauryl alcohol
Sodium sulfovinate, the mixture of coconut oleoyl amine DEA.
Further, the preservative is Methylisothiazolinone, the mixture of iodine propilolic alcohol butyl mephenesin Carbamate.
Further, the chelating agen is acrylic aldehyde/acrylic copolymer, the mixture of EDETATE SODIUM.
Further, the PH regulators are potassium hydroxide, the mixture of citric acid.
Further, the pigment is CI 19140, the mixture of CI 10020.
Wherein, described Flos Matricariae chamomillae flavone is prepared by the following method and forms：
(1) the natural Flos Matricariae chamomillae petal of 1 part of picked by hand is weighed, by 1:The petal is immersed 80% by 10 solid-liquid ratio
Ethanol soaks 30min；
(2) mixed liquor of step (1) is placed in heating and refluxing extraction 1.5h in water-bath, is filtered, collect filtrate, filtering residue uses 8
80% ethanol of amount repeats to extract once again, merges filtrate twice；
(3) by the filtrate reduced in volume in step (2) to without alcohol taste, concentrated solution is inhaled by AB-8 macroporous resin column
Attached, first with distillation water elution, then 60% ethanol elution is used, collect 60% ethanol elution, Flos Matricariae chamomillae is obtained after concentrating under reduced pressure yellow
Ketone.
Further, the preparation method of the hydrosol is as follows：
(1) deionized water in formula is weighed, 75-80 DEG C is heated to, surfactant, chela under stirring condition, is added according to this
Mixture, glycine, Polyethylene Glycol -90M, dipropylene glycol, homogenizing, to being completely dissolved；
(2) addition essence, UVNUL MS-40, Flos Matricariae chamomillae flavone, pigment, preservative when being cooled to 40-45 DEG C, stirring, homogenizing,
PH is adjusted, after being down to room temperature, Flos Matricariae chamomillae petal is added.
Research shows that Flos Matricariae chamomillae flavone is to gram-negative bacteria, enterobacter cloacae, streptococcus pyogenes, staphylococcus aureuses
Have good inhibitory action (《The antibacterial activity of Flos Chrysanthemi quintessence oil and chemical composition》, Sun Jie), while Flos Matricariae chamomillae flavone is to hexichol
For bitterness acyl group (DPPH), hydroxy radical (OH) and 2,2- azino-two (3- ethyls-benzothiazole -6- sulfonic acid) di-ammonium salts
Free radical also have good scavenging action (《In Flos Matricariae chamomillae, polysaccharide is ground with flavone gruff bring up substance antioxidation and suppression nitrosification
Study carefully》, Xia Na, Tao Haiyan), there is good antibacterial and antioxidant effect.The present invention passes through to add Flos Matricariae chamomillae Huang in bath gel
Ketone, is allow product that there is good fungistatic effect, and is used with the Flos Matricariae chamomillae petal compounding collaboration of presence in bath gel itself,
Fungistatic effect is more notable.
Petal selected by of the invention is got in morning band dew picked by hand, especially selects tender flower, per piece
Petal thickness is uniform, and frivolous softness farthest remains the beneficiating ingredient in Flos Matricariae chamomillae petal.
The invention has the advantages that：The anthemidis flos of natural picked by hand is added in the bath gel of the present invention
Lobe, the natural aromatic and petal of Flos Matricariae chamomillae are massaged to the tenderness of skin surface, whole bathing experience can be made full of temperament and interest, relaxed
Slow mood, discharges pressure, and is conducive to the further removal of skin dirt；Further, add in formula by fresh ocean
The Flos Matricariae chamomillae flavone that Flos Chrysanthemi is extracted, suffers from good inhibition to the antibacterial and free radical of human body surface, makes this product exist
While there is good cleaning moistening effect, moreover it is possible to long-acting bacteriostatic, improve allergy skin, life-time service can slow down skin
Daily aging course, allows skin gradually to recover young vigor.
Description of the drawings
Fig. 1 is impact figure of the test sample to pathogen growth curve
Specific embodiment
The integral production method of bath gel of the present invention is as follows：
(1) deionized water in formula is weighed, 75-80 DEG C is heated to, surfactant, chela under stirring condition, is added according to this
Mixture, glycine, Polyethylene Glycol -90M, dipropylene glycol, homogenizing, to being completely dissolved；
(2) addition essence, UVNUL MS-40, Flos Matricariae chamomillae flavone, pigment, preservative when being cooled to 40-45 DEG C, stirring, homogenizing,
PH is adjusted, after being down to room temperature, Flos Matricariae chamomillae petal is added.
Finally, according in the preparation method, the difference of each component content can specifically be divided into following 5 embodiments：
Embodiment 1
Embodiment 2
| Component | Weight portion/part |
| Natural Flos Matricariae chamomillae petal | 5 |
| Flos Matricariae chamomillae flavone | 0.08 |
| Sodium laureth sulfate | 10 |
| Cocamido propyl betaine | 5 |
| Sodium lauryl sulfate | 8 |
| Coconut oleoyl amine DEA | 2 |
| Methylisothiazolinone | 0.008 |
| Iodine propilolic alcohol butyl mephenesin Carbamate | 0.05 |
| Acrylic aldehyde/acrylic copolymer | 0.1 |
| EDETATE SODIUM | 0.1 |
| Potassium hydroxide | 0.04 |
| Citric acid | 0.06 |
| Dipropylene glycol | 10 |
| Polyethylene Glycol -90M | 1.5 |
| UVNUL MS-40 | 0.07 |
| Glycine | 0.1 |
| Essence | 0.2 |
| CI19140 | 0.04 |
| CI10020 | 0.06 |
| Deionized water | 58 |
Embodiment 3
Embodiment 4
| Component | Weight portion/part |
| Natural Flos Matricariae chamomillae petal | 3 |
| Flos Matricariae chamomillae flavone | 0.035 |
| Sodium laureth sulfate | 6.25 |
| Cocamido propyl betaine | 3.5 |
| Sodium lauryl sulfate | 4.25 |
| Coconut oleoyl amine DEA | 0.875 |
| Methylisothiazolinone | 0.0035 |
| Iodine propilolic alcohol butyl mephenesin Carbamate | 0.0275 |
| Acrylic aldehyde/acrylic copolymer | 0.0625 |
| EDETATE SODIUM | 0.0625 |
| Potassium hydroxide | 0.025 |
| Citric acid | 0.0375 |
| Dipropylene glycol | 6.25 |
| Polyethylene Glycol -90M | 0.525 |
| UVNUL MS-40 | 0.035 |
| Glycine | 0.0625 |
| Essence | 0.125 |
| CI19140 | 0.0215 |
| CI10020 | 0.041 |
| Deionized water | 75 |
Embodiment 5
| Component | Weight portion/part |
| Natural Flos Matricariae chamomillae petal | 4 |
| Flos Matricariae chamomillae flavone | 0.065 |
| Sodium laureth sulfate | 8.75 |
| Cocamido propyl betaine | 4.5 |
| Sodium lauryl sulfate | 6.75 |
| Coconut oleoyl amine DEA | 1.625 |
| Methylisothiazolinone | 0.0065 |
| Iodine propilolic alcohol butyl mephenesin Carbamate | 0.0425 |
| Acrylic aldehyde/acrylic copolymer | 0.0875 |
| EDETATE SODIUM | 0.0875 |
| Potassium hydroxide | 0.035 |
| Citric acid | 0.0525 |
| Dipropylene glycol | 8.75 |
| Polyethylene Glycol -90M | 1.175 |
| UVNUL MS-40 | 0.0575 |
| Glycine | 0.0875 |
| Essence | 0.175 |
| Pigment | 0.0875 |
| CI19140 | 0.0424 |
| CI10020 | 0.0451 |
| Deionized water | 64 |
Comparative example 1
Comparative example 2
| Component | Weight portion/part |
| Natural Flos Matricariae chamomillae petal | 3 |
| Sodium laureth sulfate | 5.5 |
| Cocamido propyl betaine | 4 |
| Sodium lauryl sulfate | 5.5 |
| Coconut oleoyl amine DEA | 1.25 |
| Methylisothiazolinone | 0.005 |
| Iodine propilolic alcohol butyl mephenesin Carbamate | 0.035 |
| Acrylic aldehyde/acrylic copolymer | 0.075 |
| EDETATE SODIUM | 0.075 |
| Potassium hydroxide | 0.03 |
| Citric acid | 0.045 |
| Dipropylene glycol | 7.5 |
| Polyethylene Glycol -90M | 1.5 |
| UVNUL MS-40 | 0.045 |
| Glycine | 0.075 |
| Essence | 0.15 |
| CI19140 | 0.035 |
| CI10020 | 0.04 |
| Deionized water | 74 |
Efficacy validation
1) performance of keeping humidity test
Following EXPERIMENTAL DESIGN table 1 formulation samples each 10g is weighed respectively, and uniform application is 40* in 6cm adhesive plasters, size is stained with
In 25 weighing botle, do not add a cover, be placed in 29 DEG C, during relative humidity (RH) is for 40% ± 5% constant temperature and humidity plant incubator, per
Took out every 3 hours and weigh, as shown in Table 1, parallel test 3 times calculates average percentage of water loss to weighing results according to the following formula：
Percentage of water loss %=W1-W2/W1-W × 100%；
Wherein, W1 is the weighing botle weight of sample before experiment, gram；
W2 is sample machine weighing botle weight after specific time interval, gram；
W is weighing botle weight, gram.
Table 1：Bath gel and the performance of keeping humidity test and comparison of like product prepared by embodiment, comparative example
The moistening effect that bath gel of the present invention be can be seen that by the experimental data table of embodiment is good, and moistening effect will
Certain brand bath gel better than same type；Lack the bath gel product of fresh Flos Matricariae chamomillae petal and Flos Matricariae chamomillae flavone respectively, which is protected
Bath gel finished product made by moist comparative example to be less than.And in the complete embodiment of two kinds of compositions, Flos Matricariae chamomillae flavone with fresh
Petal composition Synergistic, have the moistening effect of bath gel of the present invention and be obviously improved.
2) antioxygenic property test：
Determined with spectrophotometer method, plus the decline that OD514 absorbs after antioxidant represents which is eliminated to organic free radical
Ability.DPPH is first dissolved with a small amount of ethanol solution, and is configured to 1 × 10-4mol/L with 10% ethanol, is kept in dark place.Take
2molDPPH solution, adds 2mL bath gel to be sufficiently mixed, determines which in 514nm (maximum absorption wave strong point) after 30 minutes
OD514, to remove bath gel amount needed for DPPH50% i.e. IC50 as non-oxidizability index.Clearance rate P calculates available following formula：
P=[A 0- (A i-A j)]/A 0
In formula, the absorbance of A0=2mLDPPH solution+2mL solution；Ai=2mLDPPH solution+2mL the present embodiment 1-5 and
The absorbance of comparative example 1-4 bath gel；The absorbance of Aj=2mL the present embodiment bath gel+2mL solvents.
Test result indicate that, the external each embodiment for removing DPPH free radicals (1 × 10-4mol/L) 50% i.e. needed for IC50
And the bath gel amount needed for comparative example is as shown in the table：
Table 2：Antioxygenic property table
As can be seen that compared with comparative example and certain bath gel, bath gel of the present invention has prominent external removing free radical
Ability, the performance with good antioxidation delay skin aging.
3) fungistatic effect test
In order to study inhibition of the bath gel product of the present invention that with the addition of Flos Matricariae chamomillae flavone to body surface antibacterial, take
Same amount of embodiment 3, comparative example 1, the sample of comparative example 2, test to identical bacterium colony sample, bacterium colony in analysis each group
Growth curve, as shown in Figure 1.
The result shown from Fig. 1 can be seen that the bath gel fungistatic effect of the present invention is good, and it is yellow to add Flos Matricariae chamomillae simultaneously
Due to both Synergistics after ketone and fresh Flos Matricariae chamomillae petal, its fungistatic effect will be obviously improved.
4) using effect：
25-35 year is respectively chosen for embodiment 1-5, comparative example 1-2 and certain brand bath gel different degrees of skin mistake
The women of quick, prurituss is each 100.
Usage cycles 2 months, take a shower using above-mentioned bath gel product every time, are tied after observation using effect as follows
Really：
3 using effect of table is counted
Shown using result, after the bath gel bath prepared by the present invention, skin smooth moistens, and life-time service can suppress flesh
Skin aging makes which recover elasticity in the past, while the antibacterial of body surface can be suppressed, builds the skin environment of health, using effect
Better than certain the brand bath gel on market；And under conditions of Flos Matricariae chamomillae flavone or Flos Matricariae chamomillae petal is lacked, two kinds of compositions without
Method Synergistic so as to which beneficial effect is substantially reduced.
Embodiment described above only expresses the several embodiments of the present invention, and its description is more concrete and detailed, but simultaneously
Therefore the restriction to the scope of the claims of the present invention can not be interpreted as.It should be pointed out that for one of ordinary skill in the art
For, without departing from the inventive concept of the premise, some deformations and improvement can also be made, these belong to the guarantor of the present invention
Shield scope.
Claims (9)
1. a kind of natural chamomile hydrosol rich in picked by hand, the hydrosol mainly include the natural anthemidis flos of picked by hand
Lobe 2-5 parts, Flos Matricariae chamomillae flavone 0.02-0.08 parts.
2. a kind of natural chamomile hydrosol rich in picked by hand according to claim 1, it is characterised in that the hydrosol
Also include the composition of following weight portion：
3. a kind of natural chamomile hydrosol rich in picked by hand according to claim 2, it is characterised in that the surface
Activating agent be sodium laureth sulfate, cocamido propyl betaine, sodium lauryl sulfate, coconut oleoyl amine DEA mixed
Compound.
4. a kind of natural chamomile hydrosol rich in picked by hand according to claim 2, it is characterised in that the anti-corrosion
Agent is Methylisothiazolinone, the mixture of iodine propilolic alcohol butyl mephenesin Carbamate.
5. a kind of natural chamomile hydrosol rich in picked by hand according to claim 2, it is characterised in that the chelating
Agent is acrylic aldehyde/acrylic copolymer, the mixture of EDETATE SODIUM.
6. a kind of natural chamomile hydrosol rich in picked by hand according to claim 2, it is characterised in that the PH is adjusted
Section agent is potassium hydroxide, the mixture of citric acid.
7. a kind of natural chamomile hydrosol rich in picked by hand according to claim 2, it is characterised in that the pigment
For CI 19140, the mixture of CI 10020.
8. a kind of natural chamomile hydrosol rich in picked by hand according to claim 1, it is characterised in that the ocean is sweet
Chrysanthemum flavone is prepared by the following method and forms：
(1) the natural Flos Matricariae chamomillae petal of 1 part of picked by hand is weighed, by 1:The petal is immersed 80% ethanol by 10 solid-liquid ratio
Immersion 30min；
(2) mixed liquor of step (1) is placed in heating and refluxing extraction 1.5h in water-bath, is filtered, collect filtrate, filtering residue is measured with 8 times
80% ethanol repeats to extract once, merges filtrate twice；
(3) by the filtrate reduced in volume of step (2) to without alcohol taste, concentrated solution is adsorbed by AB-8 macroporous resin column, is first used
Distillation water elution, then 60% ethanol elution is used, 60% ethanol elution is collected, Flos Matricariae chamomillae flavone after concentrating under reduced pressure, is obtained.
9. a kind of natural chamomile hydrosol rich in picked by hand according to claim 1-8, it is characterised in which is prepared
Method is as follows：
(1) deionized water in formula is weighed, 75-80 DEG C is heated to, surfactant, chelating under stirring condition, is added according to this
Agent, glycine, Polyethylene Glycol -90M, dipropylene glycol, homogenizing, to being completely dissolved；
(2) essence, UVNUL MS-40, Flos Matricariae chamomillae flavone, pigment, preservative are added when being cooled to 40-45 DEG C, and stirring, homogenizing are adjusted
PH, adds Flos Matricariae chamomillae petal after being down to room temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887135.3A CN106491411A (en) | 2016-10-11 | 2016-10-11 | One kind is rich in natural Flos Matricariae chamomillae petal and its hydrosol |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610887135.3A CN106491411A (en) | 2016-10-11 | 2016-10-11 | One kind is rich in natural Flos Matricariae chamomillae petal and its hydrosol |
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| Publication Number | Publication Date |
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| CN106491411A true CN106491411A (en) | 2017-03-15 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108210538A (en) * | 2018-04-09 | 2018-06-29 | 嘉兴市寒疆芳草生物科技有限公司 | A kind of chamomile hydrosol extracting method of anti-candida albicans |
| CN111714423A (en) * | 2020-07-31 | 2020-09-29 | 广州环亚化妆品科技有限公司 | Traditional Chinese medicine composition extract with effects of resisting aging and relieving allergy, and preparation method and application thereof |
-
2016
- 2016-10-11 CN CN201610887135.3A patent/CN106491411A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108210538A (en) * | 2018-04-09 | 2018-06-29 | 嘉兴市寒疆芳草生物科技有限公司 | A kind of chamomile hydrosol extracting method of anti-candida albicans |
| CN111714423A (en) * | 2020-07-31 | 2020-09-29 | 广州环亚化妆品科技有限公司 | Traditional Chinese medicine composition extract with effects of resisting aging and relieving allergy, and preparation method and application thereof |
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