CN109072286B - 细胞裂解和核酸扩增的方法 - Google Patents

细胞裂解和核酸扩增的方法 Download PDF

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Publication number
CN109072286B
CN109072286B CN201780022472.9A CN201780022472A CN109072286B CN 109072286 B CN109072286 B CN 109072286B CN 201780022472 A CN201780022472 A CN 201780022472A CN 109072286 B CN109072286 B CN 109072286B
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aqueous composition
volume
nucleic acid
buffer
lysis buffer
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Chinese (zh)
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CN109072286A (zh
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格雷戈里·W·西顿
尼尔·帕西
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3M Innovative Properties Co
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3M Innovative Properties Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201780022472.9A 2016-04-08 2017-03-24 细胞裂解和核酸扩增的方法 Active CN109072286B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662319876P 2016-04-08 2016-04-08
US62/319,876 2016-04-08
PCT/US2017/023951 WO2017176469A1 (en) 2016-04-08 2017-03-24 Process for cell lysis and nucleic acid amplification

Publications (2)

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CN109072286A CN109072286A (zh) 2018-12-21
CN109072286B true CN109072286B (zh) 2023-08-11

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US (1) US11168352B2 (enExample)
EP (1) EP3440223B1 (enExample)
JP (1) JP6979409B2 (enExample)
CN (1) CN109072286B (enExample)
WO (1) WO2017176469A1 (enExample)

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AU2021400825A1 (en) * 2020-12-16 2023-07-06 Biofire Diagnostics, Llc Systems, methods, and apparatuses for concentration and identification of a microorganism from blood
KR20240070556A (ko) 2021-09-01 2024-05-21 쓰리엠 이노베이티브 프로퍼티즈 컴파니 피분석물을 검출하는 방법 및 조성물
EP4163369A1 (en) * 2021-10-08 2023-04-12 AXAGARIUS GmbH & Co. KG Use of mixtures of polyvinylpyrrolidone and ammonium sulfate in the isolation of nucleic acids

Citations (4)

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JPH08149974A (ja) * 1994-11-30 1996-06-11 Q P Corp Ｐｃｒ用増菌培地
US5620869A (en) * 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
US5726021A (en) * 1994-06-16 1998-03-10 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
CN105462957A (zh) * 2014-09-30 2016-04-06 鸿慧(上海)生物科技有限公司 一种裂解组合物及其用途、试剂盒、利用该裂解组合物制备核酸的方法、核酸分析的方法

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JPH056607A (ja) 1991-06-28 1993-01-14 Sony Corp デイスクプレーヤ
AU710996B2 (en) * 1996-05-22 1999-10-07 Gen-Probe Incorporated Compositions and methods for the detection of mycobacterium kansasii
JP5006607B2 (ja) 2006-09-19 2012-08-22 栄研化学株式会社 核酸増幅反応における阻害を回避する方法
US20140038174A1 (en) * 2011-04-26 2014-02-06 Longhorn Vaccines And Diagnostics, Llc Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples
KR101039521B1 (ko) * 2008-02-01 2011-06-08 주식회사 인트론바이오테크놀로지 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물
JP2011097828A (ja) 2008-07-09 2011-05-19 Beckman Coulter Inc 全血試料の前処理方法及び核酸増幅方法
WO2011103539A1 (en) * 2010-02-19 2011-08-25 Argylla Technologies Isolation of biomolecules from biological samples
EP2634254A1 (en) * 2012-02-29 2013-09-04 QIAGEN GmbH Method for isolating nucleic acids from a food sample
JP6402347B2 (ja) * 2012-03-29 2018-10-10 株式会社シノテスト 核酸の検出又は定量方法及び検出又は定量用試薬キット
US20150225712A1 (en) 2012-08-21 2015-08-13 Qiagen Gmbh Method for isolating nucleic acids from a formaldehyde releaser stabilized sample
EP2730653A1 (en) * 2012-11-07 2014-05-14 QIAGEN GmbH Method for lysing a fixed biological sample
EP3189162A1 (en) 2014-09-02 2017-07-12 Pharmozyme, Inc. Genetic detection platform
CN107109402B (zh) * 2014-12-23 2020-09-25 3M创新有限公司 用于减少核酸扩增抑制的组合物
WO2016183012A1 (en) * 2015-05-11 2016-11-17 3M Innovative Properties Company Composition for reducing inhibition of nucleic acid amplification

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5726021A (en) * 1994-06-16 1998-03-10 Becton Dickinson And Company Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors
JPH08149974A (ja) * 1994-11-30 1996-06-11 Q P Corp Ｐｃｒ用増菌培地
US5620869A (en) * 1995-09-28 1997-04-15 Becton, Dickinson And Company Methods for reducing inhibition of nucleic acid amplification reactions
CN105462957A (zh) * 2014-09-30 2016-04-06 鸿慧(上海)生物科技有限公司 一种裂解组合物及其用途、试剂盒、利用该裂解组合物制备核酸的方法、核酸分析的方法

Also Published As

Publication number Publication date
JP2019517778A (ja) 2019-06-27
CN109072286A (zh) 2018-12-21
US20190112637A1 (en) 2019-04-18
US11168352B2 (en) 2021-11-09
WO2017176469A1 (en) 2017-10-12
EP3440223A1 (en) 2019-02-13
BR112018069624A2 (pt) 2019-10-01
JP6979409B2 (ja) 2021-12-15
EP3440223B1 (en) 2020-12-23

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