BR112018069624A2 - método de amplificação de ácido nucleico e tampão de lise - Google Patents
método de amplificação de ácido nucleico e tampão de liseInfo
- Publication number
- BR112018069624A2 BR112018069624A2 BR112018069624A BR112018069624A BR112018069624A2 BR 112018069624 A2 BR112018069624 A2 BR 112018069624A2 BR 112018069624 A BR112018069624 A BR 112018069624A BR 112018069624 A BR112018069624 A BR 112018069624A BR 112018069624 A2 BR112018069624 A2 BR 112018069624A2
- Authority
- BR
- Brazil
- Prior art keywords
- nucleic acid
- acid amplification
- aqueous composition
- enrichment culture
- lysis buffer
- Prior art date
Links
- 238000003199 nucleic acid amplification method Methods 0.000 title abstract 4
- 239000012139 lysis buffer Substances 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 abstract 7
- 238000000034 method Methods 0.000 abstract 5
- 230000003321 amplification Effects 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 3
- 102000039446 nucleic acids Human genes 0.000 abstract 3
- 108020004707 nucleic acids Proteins 0.000 abstract 3
- 230000009089 cytolysis Effects 0.000 abstract 2
- 238000009472 formulation Methods 0.000 abstract 1
- 239000002198 insoluble material Substances 0.000 abstract 1
- 230000014759 maintenance of location Effects 0.000 abstract 1
- 230000001404 mediated effect Effects 0.000 abstract 1
- 244000005700 microbiome Species 0.000 abstract 1
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
a presente revelação fornece um método de amplificação de ácidos nucleicos. o método compreende formar uma cultura de enriquecimento mediante o contato de uma amostra com um meio nutritivo que tem uma formulação que não inclui um componente de tampão de fosfato; manter a cultura de enriquecimento durante um período de tempo a uma temperatura que facilita o crescimento de um micro-organismo alvo; após manter a cultura de enriquecimento, formar uma composição aquosa mediante a mistura de um primeiro volume da cultura de enriquecimento com um segundo volume de um tampão de lise; colocar em contato a composição aquosa com uma quantidade eficaz de um material insolúvel em água que sequestra uma substância que interfere com uma reação de amplificação de ácidos nucleicos mediada por polimerase; submeter a composição aquosa a um processo de lise térmica e, após submeter a composição aquosa ao processo de lise térmica, submeter uma porção da composição aquosa a um processo de amplificação de ácidos nucleicos. uma composição para um tampão de lise é revelada também.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662319876P | 2016-04-08 | 2016-04-08 | |
| PCT/US2017/023951 WO2017176469A1 (en) | 2016-04-08 | 2017-03-24 | Process for cell lysis and nucleic acid amplification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BR112018069624A2 true BR112018069624A2 (pt) | 2019-10-01 |
Family
ID=58633077
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112018069624A BR112018069624A2 (pt) | 2016-04-08 | 2017-03-24 | método de amplificação de ácido nucleico e tampão de lise |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US11168352B2 (pt) |
| EP (1) | EP3440223B1 (pt) |
| JP (1) | JP6979409B2 (pt) |
| CN (1) | CN109072286B (pt) |
| BR (1) | BR112018069624A2 (pt) |
| WO (1) | WO2017176469A1 (pt) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116724122A (zh) * | 2020-12-16 | 2023-09-08 | 拜奥法尔诊断有限责任公司 | 用于浓缩和鉴定血液中微生物的系统、方法和设备 |
| WO2023031691A1 (en) | 2021-09-01 | 2023-03-09 | 3M Innovative Properties Company | Compositions and methods of detecting analytes |
| EP4163369A1 (en) * | 2021-10-08 | 2023-04-12 | AXAGARIUS GmbH & Co. KG | Use of mixtures of polyvinylpyrrolidone and ammonium sulfate in the isolation of nucleic acids |
Family Cites Families (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH056607A (ja) | 1991-06-28 | 1993-01-14 | Sony Corp | デイスクプレーヤ |
| US5712095A (en) | 1994-06-16 | 1998-01-27 | Becton Dickinson And Company | Rapid and sensitive detection of antibiotic-resistant mycobacteria using oligonucleotide probes specific for ribosomal RNA precursors |
| JPH08149974A (ja) * | 1994-11-30 | 1996-06-11 | Q P Corp | Ｐｃｒ用増菌培地 |
| US5620869A (en) * | 1995-09-28 | 1997-04-15 | Becton, Dickinson And Company | Methods for reducing inhibition of nucleic acid amplification reactions |
| CA2255624C (en) | 1996-05-22 | 2007-09-04 | Gen-Probe Incorporated | Compositions and methods for the detection of mycobacterium kansasii |
| JP5006607B2 (ja) | 2006-09-19 | 2012-08-22 | 栄研化学株式会社 | 核酸増幅反応における阻害を回避する方法 |
| US20140038174A1 (en) * | 2011-04-26 | 2014-02-06 | Longhorn Vaccines And Diagnostics, Llc | Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples |
| KR101039521B1 (ko) * | 2008-02-01 | 2011-06-08 | 주식회사 인트론바이오테크놀로지 | 금속 이온에 의해 발생하는 ｐｃｒ 저해 및 비특이 증폭을완화시키거나 막을 수 있는 에틸렌글리콜테트라아세틱 산을킬레이트제로 포함하는 ｐｃｒ 반응용 조성물 |
| JP2011097828A (ja) | 2008-07-09 | 2011-05-19 | Beckman Coulter Inc | 全血試料の前処理方法及び核酸増幅方法 |
| US20120003710A1 (en) | 2010-02-19 | 2012-01-05 | Barbara Dawn Leinweber | Isolation of Biomolecules from Biological Samples |
| EP2634254A1 (en) * | 2012-02-29 | 2013-09-04 | QIAGEN GmbH | Method for isolating nucleic acids from a food sample |
| WO2013147043A1 (ja) | 2012-03-29 | 2013-10-03 | 株式会社シノテスト | 核酸の検出又は定量方法及び検出又は定量用試薬キット |
| US20150225712A1 (en) | 2012-08-21 | 2015-08-13 | Qiagen Gmbh | Method for isolating nucleic acids from a formaldehyde releaser stabilized sample |
| EP2730653A1 (en) | 2012-11-07 | 2014-05-14 | QIAGEN GmbH | Method for lysing a fixed biological sample |
| EP3189162A1 (en) | 2014-09-02 | 2017-07-12 | Pharmozyme, Inc. | Genetic detection platform |
| CN105462957A (zh) * | 2014-09-30 | 2016-04-06 | 鸿慧(上海)生物科技有限公司 | 一种裂解组合物及其用途、试剂盒、利用该裂解组合物制备核酸的方法、核酸分析的方法 |
| JP6650944B2 (ja) | 2014-12-23 | 2020-02-19 | スリーエム イノベイティブ プロパティズ カンパニー | 核酸増幅の阻害を低減するための組成物 |
| US10619189B2 (en) | 2015-05-11 | 2020-04-14 | 3M Innovative Properties Company | Composition for reducing inhibition of nucleic acid amplification |
-
2017
- 2017-03-24 EP EP17719745.6A patent/EP3440223B1/en active Active
- 2017-03-24 BR BR112018069624A patent/BR112018069624A2/pt active Search and Examination
- 2017-03-24 JP JP2018552671A patent/JP6979409B2/ja active Active
- 2017-03-24 CN CN201780022472.9A patent/CN109072286B/zh active Active
- 2017-03-24 US US16/087,750 patent/US11168352B2/en active Active
- 2017-03-24 WO PCT/US2017/023951 patent/WO2017176469A1/en active Application Filing
Also Published As
| Publication number | Publication date |
|---|---|
| CN109072286A (zh) | 2018-12-21 |
| JP2019517778A (ja) | 2019-06-27 |
| CN109072286B (zh) | 2023-08-11 |
| EP3440223B1 (en) | 2020-12-23 |
| US11168352B2 (en) | 2021-11-09 |
| US20190112637A1 (en) | 2019-04-18 |
| EP3440223A1 (en) | 2019-02-13 |
| JP6979409B2 (ja) | 2021-12-15 |
| WO2017176469A1 (en) | 2017-10-12 |
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| B06W | Patent application suspended after preliminary examination (for patents with searches from other patent authorities) chapter 6.23 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B09X | Republication of the decision to grant [chapter 9.1.3 patent gazette] |