CN109072215A - 一种头孢菌素c酰化酶突变体及其应用 - Google Patents

一种头孢菌素c酰化酶突变体及其应用 Download PDF

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CN109072215A
CN109072215A CN201780023015.1A CN201780023015A CN109072215A CN 109072215 A CN109072215 A CN 109072215A CN 201780023015 A CN201780023015 A CN 201780023015A CN 109072215 A CN109072215 A CN 109072215A
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seq
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mutant
cephalosporin
cpc
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CN109072215B (zh
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杨晟
王金刚
蒋宇
陈舒明
梁岩
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Shanxi Shuangyan Biotechnology Co ltd
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Shanghai Xingwei Biotechnology Co ltd
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Abstract

提供了一种通过点突变法构建的头孢菌素C酰化酶突变体，其氨基酸序列分别为SEQ ID NOs:3‑12。相比野生型头孢菌素C酰化酶，这些突变体底物特异性和酶活力得到了提高，能用于以头孢菌素C为底物催化生产7‑氨基头孢烷酸。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN201780023015.1A 2017-03-15 2017-03-15 一种头孢菌素c酰化酶突变体及其应用 Active CN109072215B (zh)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110129305A (zh) * 2019-05-28 2019-08-16 河北凯恩利生物技术有限公司 一种用于制备7-aca的头孢菌素c酰化酶突变体
CN111172142A (zh) * 2020-02-14 2020-05-19 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体
CN112662655A (zh) * 2020-12-29 2021-04-16 山东金城柯瑞化学有限公司 头孢菌素c酰化酶突变体及其制备方法和应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102405289B1 (ko) * 2021-12-24 2022-06-07 아미코젠주식회사 세팔로스포린 c 아실라제 활성을 가지는 폴리펩타이드 및 이의 용도

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CN101240285A (zh) * 2008-03-19 2008-08-13 清华大学 一种头孢菌素c酰化酶及其载体和应用
CN102925423A (zh) * 2012-11-16 2013-02-13 清华大学 一种突变头孢菌素c酰化酶
CN102978192A (zh) * 2012-12-25 2013-03-20 湖南福来格生物技术有限公司 突变型头孢菌素c酰化酶及其制备方法和转化7-aca的方法
CN103060298A (zh) * 2012-12-31 2013-04-24 安徽丰原基因工程技术有限公司 一种头孢菌素c酰化酶突变体及其编码基因与应用
CN105087533A (zh) * 2015-09-30 2015-11-25 湖南福来格生物技术有限公司 一种青霉素g酰化酶的突变体及其制备方法和应用
CN105543201A (zh) * 2016-02-23 2016-05-04 上海星维生物技术有限公司 一种头孢菌素c酰化酶突变体
CN106119233A (zh) * 2016-07-01 2016-11-16 清华大学 头孢菌素酰化酶突变体及其编码基因与应用

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* Cited by examiner, † Cited by third party
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AU2002256860A1 (en) * 2001-03-14 2002-09-24 Klaus-Peter Koller Variant glutaryl amidase (cephalosporin acylase) and uses thereof
KR101728906B1 (ko) * 2013-01-21 2017-04-20 아미코젠주식회사 세파계 항생제 원료물질(7-aca)을 생산하기 위한 변이효소

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101240285A (zh) * 2008-03-19 2008-08-13 清华大学 一种头孢菌素c酰化酶及其载体和应用
CN102925423A (zh) * 2012-11-16 2013-02-13 清华大学 一种突变头孢菌素c酰化酶
CN102978192A (zh) * 2012-12-25 2013-03-20 湖南福来格生物技术有限公司 突变型头孢菌素c酰化酶及其制备方法和转化7-aca的方法
CN103060298A (zh) * 2012-12-31 2013-04-24 安徽丰原基因工程技术有限公司 一种头孢菌素c酰化酶突变体及其编码基因与应用
CN105087533A (zh) * 2015-09-30 2015-11-25 湖南福来格生物技术有限公司 一种青霉素g酰化酶的突变体及其制备方法和应用
CN105543201A (zh) * 2016-02-23 2016-05-04 上海星维生物技术有限公司 一种头孢菌素c酰化酶突变体
CN106119233A (zh) * 2016-07-01 2016-11-16 清华大学 头孢菌素酰化酶突变体及其编码基因与应用

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110129305A (zh) * 2019-05-28 2019-08-16 河北凯恩利生物技术有限公司 一种用于制备7-aca的头孢菌素c酰化酶突变体
CN110129305B (zh) * 2019-05-28 2022-10-28 河北凯恩利生物技术有限公司 一种用于制备7-aca的头孢菌素c酰化酶突变体
CN111172142A (zh) * 2020-02-14 2020-05-19 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体
CN111172142B (zh) * 2020-02-14 2021-09-28 上海陶宇晟生物技术有限责任公司 一种热稳定性高的头孢菌素c酰化酶突变体
CN112662655A (zh) * 2020-12-29 2021-04-16 山东金城柯瑞化学有限公司 头孢菌素c酰化酶突变体及其制备方法和应用

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