CN109007263A - A kind of paper mulberry ensilage and preparation method thereof - Google Patents

A kind of paper mulberry ensilage and preparation method thereof Download PDF

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Publication number
CN109007263A
CN109007263A CN201810874144.8A CN201810874144A CN109007263A CN 109007263 A CN109007263 A CN 109007263A CN 201810874144 A CN201810874144 A CN 201810874144A CN 109007263 A CN109007263 A CN 109007263A
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parts
feed
mixture
paper
leavening
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黎庶凯
曾俊
黄世洋
罗荣太
包熙旺
梁琼
帕明秀
卢肇高
张友泓
陈思业
黄静丽
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Guangxi Zhuang Autonomous Region Forage Workstation
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A23K10/18Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/142Amino acids; Derivatives thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/163Sugars; Polysaccharides
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/22Compounds of alkali metals
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/26Compounds containing phosphorus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/30Oligoelements
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/10Feeding-stuffs specially adapted for particular animals for ruminants
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Polymers & Plastics (AREA)
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Abstract

The invention belongs to feed for sheep technical fields, it is related to a kind of paper mulberry ensilage and preparation method thereof, is mainly made of raw material from the following weight: 60-75 parts of feed with paper-mulberry leaf, 20-35 parts of corn stover, careless No. 1 18-25 parts of the imperatoria in osmanthus, 20-25 parts of feed mulberry leaf, 6-10 parts of Canterburybells, 2-4 parts of composite mineral matter, -2 parts of amino acid 1,0.8-1.5 parts of salt, 6-10 parts of sugar and 1-3 part of leavening;The leavening is the mixture of the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and chaetomium globosum, wherein, the weight part ratio of the sugared sporangium of the green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and chaetomium globosum are as follows: 2-3:2-3:3-6:2-4:1-3.The present invention has the function of that improving feed digestion absorbs, improves palatability and proliferation Bifidobacterium and strengthen immunity, and preparation method provided by the invention is simple, easy to spread.

Description

A kind of paper mulberry ensilage and preparation method thereof
[technical field]
The invention belongs to feed for sheep technical fields, and in particular to a kind of paper mulberry ensilage and preparation method thereof.
[background technique]
After crops harvesting, a large amount of agricultural crop straw cup is discarded or burns, this way not only waste of resource, but also pollution ring Border affects the sustainable development of economic society to a certain extent.By the way that stalk is crushed progress ensiling, ammonification, rubs a microbial Raise livestock afterwards can both save feed cost, and stalk can also be made to realize abdomen returning to the field by excrement of animals, promote agricultural good Property circulation, be a kind of Land use systems that better results.
Ensilage is a kind of feed, is formed, is mainly used for after sealing, fermenting by the plant feed more than moisture content Sheep is ruminated in nursing.Ensilage is better than dry feed than fresh feed shelf-stable, nutritional ingredient.In addition, ensilage storage land occupation It is few, there is no fire problem.The development of ensilage, be derived from traditional agriculture Commercial cultivation mode the shortcomings that.In crop plant cells wall The high-molecular compounds such as cellulose rich in, lignin, since sheep body itself is without stem molecules such as digestion cellulose, lignin The enzyme of compound, thus the substances such as digestibility and utilization cellulose, lignin can not be decomposed, herbvore sheep passes through the symbiosis in its alimentary canal Microorganism is by cellulose decomposition, to be absorbed and utilized.Under normal conditions, people are in order to improve the livestocks such as sheep to ensilage Absorptivity, some is added into the drug for promoting digestion in feed class, can improve sheep although promoting the addition of drug of digestion Digestion and absorption to feed, or even its disease-resistant performance is improved, but abuse is easy to produce drug resistance, cause food safety, to the mankind Body causes greatly to damage;Meanwhile the health-care efficacy containing enhancing sheep immunity etc. in ensilage in the prior art, but imitate Fruit is unobvious.
[summary of the invention]
In view of above content, it is necessary to provide a kind of paper mulberry ensilage, which improves the digestion and absorption of sheep Rate, while also having the function of prebiotics.
In order to achieve the above objectives, the technical scheme adopted by the invention is that:
A kind of paper mulberry ensilage, is mainly made of raw material from the following weight: 60-75 parts of feed with paper-mulberry leaf, corn stover 20- 35 parts, osmanthus careless No. 1 18-25 parts of imperatoria, 20-25 parts of feed mulberry leaf, 6-10 parts of Canterburybells, 2-4 parts of composite mineral matter, amino acid 1- 2 parts, 0.8-1.5 parts of salt, 6-10 parts of sugar and 1-3 parts of leavening;The leavening be the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and the mixture of chaetomium globosum, wherein the green sugar sporangium, Neurospora sitophila, heat are fine The weight part ratio of clostridium, Melon fusarium Wilt and chaetomium globosum is 2-3:2-3:3-6:2-4:1-3.
In the present invention, further instruction, the paper mulberry ensilage are mainly made of raw material from the following weight: structure 70 parts of leaf, 30 parts of corn stover, osmanthus careless No. 1 20 parts of imperatoria, 23 parts of feed mulberry leaf, 8 parts of Canterburybells, 3 parts of composite mineral matter, ammonia 1.5 parts of base acid, 1 part of salt, 8 parts and 2 parts of leavening of sugar;The leavening is the sugared sporangium of green, Neurospora sitophila, the fine shuttle of heat Bacterium, Melon fusarium Wilt and chaetomium globosum mixture, wherein the sugared sporangium of the green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and the weight part ratio of chaetomium globosum are 2.5:2.5:5:3:2.
In the present invention, further instruction, the composite mineral matter be calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and The mixture of selenium, the calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium are with 2:2:2:0.6:0.5:0.5:0.4:0.4:0.3: 0.3 weight ratio mixes.
In the present invention, further instruction, the amino acid are the mixed of tryptophan, lysine, histidine and threonine Object is closed, the tryptophan, lysine, histidine and threonine are mixed with the weight ratio of 2:2:1.5:1.
A method of preparing paper mulberry ensilage, comprising the following steps:
(1) feed with paper-mulberry leaf, corn stover, careless No. 1 imperatoria in osmanthus, feed mulberry leaf and Canterburybells are weighed respectively by weight, it will be upper Stating raw material and cleaning respectively and be dried to moisture content is 55-75%, then by feed with paper-mulberry leaf, corn stover, the osmanthus grass 1 after drying Imperatoria, feed mulberry leaf and Canterburybells are switched to broken section that length is 1-2cm respectively, and it is broken to respectively obtain broken section of feed with paper-mulberry leaf, corn stover Section, careless No. 1 broken section of the imperatoria in osmanthus, broken section of feed mulberry leaf and broken section of Canterburybells, broken section of the above raw material is mixed, mixture I is obtained;
(2) composite mineral matter, amino acid, salt and sugar are weighed respectively by weight, then by the composite mineral matter, institute It states amino acid, the salt and the sugar to be separately added into the mixture I that step (1) obtains, and stirs so that above-mentioned substance is mixed It closes uniformly, obtains mixture II;
(3) leavening is weighed by weight, and weighs the water of 25-30 times of leavening by weight, then by the fermentation Agent is added in above-mentioned water, is sufficiently stirred so that leavening is uniformly mixed with water, obtains diluting bacterium solution;
(4) dilution bacterium solution obtained in step (3) is added in the obtained mixture II of step (2), is sufficiently stirred So that the dilution bacterium solution is uniformly mixed with the mixture II, mixture III is obtained;Then mixture III is compacted, then used Plastic film is placed in container after being sealed to ferment, and the time of fermentation is 35-45 days to get ensilage.
In the present invention, further instruction, in the preparation method of paper mulberry ensilage, the water in step (2) is nothing Bacterium water.
In the present invention, further instruction, in the preparation method of paper mulberry ensilage, the temperature of fermentation in step (4) Degree is 20-35 DEG C.
The invention has the following advantages:
1. ensilage of the present invention meets demand of the sheep to basic nutrition, while improving the palatability and digestion of sheep Absorptivity also has the function of prebiotics, specifically: its raw material is with feed with paper-mulberry leaf, corn stover, careless No. 1 imperatoria in osmanthus and feed The plant of the sheep often feeding such as mulberry leaf is basic raw material, simultaneously added with substances such as composite mineral matter and amino acid, the above raw material Containing basic nutrition ingredient needed for sheep, the ratio of each component is prepared according to the nutritional need feature of goat, is very suitable to mountain The nutritional need of sheep constitution and growth and development.Cellulose and lignin rich in plant cell wall, it is difficult to for general food Careless sheep institute digestibility and utilization, thus sheep is reduced to using plant as the mesotrophic abundant digestion and absorption of the feed of raw material, certain The waste of nutrition is caused in degree, in this regard, the present invention by added in leavening the sugared sporangium of green, Neurospora sitophila and Clostridium thermocellum has the function that degrading plant cell wall cellulose and lignin, Green sugar sporangium can produce lignin mistake Oxide enzyme, lignin peroxidase are a kind of lignin-degrading enzymes, can form free radical in lignin polymer, cause Key, which is stablized, to be deteriorated to destroy lignin macromolecular;Neurospora sitophila can produce manganese peroxidase, and manganese peroxidase passes through Oxidation has the function that phenolic lignin of degrading, and lignin peroxidase and manganese peroxidase are reached by different approach The effect of lignin degrading, thus in the case that the two exists concurrently with, the degradation to lignin can be enhanced;Clostridium thermocellum By eccrine fiber corpusculum and free cellulase to degrade to cellulose, therefore the sugared sporangium of green, eat arteries and veins well The addition of spore bacterium and Clostridium thermocellum can cellulose and lignin effectively in degrading plant cell wall, make the fibre for being difficult to digest and assimilate High-molecular compound in the cell walls such as dimension element and lignin is degraded to small molecule compound, so that it is easy to sheep digestion and absorption, into And accelerate the growth of sheep, test result is shown, after continuously feeding 30 days with ensilage of the present invention, tests the average weight of goat Increment is up to 1430g;Simultaneously because one of main degradation products of lignin are thus to play raising containing aromatic compound The effect of the palatability of sheep, test result show that the test goat average daily grazing rate fed using finished product of the present invention is up to 96%, and the average daily grazing rate of test goat for using commercially available corn silage stalk to feed is only 74%.Also contain sweet tea in leavening Cucurbit wilt bacterium and chaetomium globosum, Melon fusarium Wilt can produce inulinase, and hydrolysis of inulin can be oligofructose by inulinase, this Canterburybells in the raw material of invention provides source for inulin, and oligofructose is functional oligose, has proliferation bifid The effect of bacillus, enhancing immunity of organisms etc.;Chaetomium globosum then can produce zytase, and zytase can contain plant cell Xylan hydrolysis be xylo-oligosaccharide, xylo-oligosaccharide is all functional oligose, optionally promotes intestinal bifidobacteria Proliferation activity, while stimulating the immune system of sheep body, improve the effect of immunity, studies have shown that oligofructose and oligomeric Xylose is in certain proportion in use, have the function of synergy to immunity is improved, test result shows using the present invention The bacterium colony content of Bifidobacterium is up to 7.62log cfu/g in the goat excrement of forage feed, shows that the present invention has increment double The effect of discrimination bacillus.To sum up, the present invention solves the problems, such as to be difficult to digest and assimilate by the feed of raw material of plant, improves simultaneously The palatability of sheep also has the function of being proliferated intestinal bifidobacteria, strengthen immunity.
2. ensilage raw materials of the present invention source is wide, required laboratory apparatus is simple, and preparation method is simple, easily In popularization.
[specific embodiment]
The present invention provides a kind of paper mulberry ensilage and preparation method thereof is provided, to make the purpose of the present invention, technical side Case and effect are clearer, clear, and the present invention is described in more detail below.It should be appreciated that specific reality described herein It applies example to be only used to explain the present invention, be not intended to limit the present invention.
Embodiment 1
Paper mulberry ensilage provided by the invention is prepared by formula as below and method:
A kind of paper mulberry ensilage, is mainly made of raw material from the following weight: 60 parts of feed with paper-mulberry leaf, 20 parts of corn stover, Osmanthus No. 1 18 parts of imperatoria of grass, 20 parts of feed mulberry leaf, 6 parts of Canterburybells, 2 parts of composite mineral matter, amino acid 1 part, 0.8 part of salt, sugar 6 Part and 1 part of leavening;The leavening is the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and ball hair The mixture of shell bacterium, wherein the green sugar sporangium, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and chaetomium globosum Weight part ratio be 2:2:3:2:1.Wherein, the composite mineral matter is the mixed of calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium Object is closed, the calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium are with 2:2:2:0.6:0.5:0.5:0.4:0.4:0.3:0.3's Weight ratio mixes;The amino acid is the mixture of tryptophan, lysine, histidine and threonine, and the tryptophan relies Propylhomoserin, histidine and threonine are mixed with the weight ratio of 2:2:1.5:1.
Paper mulberry ensilage the preparation method is as follows:
(1) feed with paper-mulberry leaf, corn stover, careless No. 1 imperatoria in osmanthus, feed mulberry leaf and Canterburybells are weighed respectively by weight, it will be upper Stating raw material and cleaning respectively and be dried to moisture content is 55%, then by the feed with paper-mulberry leaf after drying, corn stover, careless No. 1 king in osmanthus Grass, feed mulberry leaf and Canterburybells are switched to broken section that length is 1cm respectively, respectively obtain broken section of feed with paper-mulberry leaf, broken section of corn stover, osmanthus Careless No. 1 broken section of imperatoria, broken section of feed mulberry leaf and broken section of Canterburybells, broken section of the above raw material is mixed, mixture I is obtained;
(2) composite mineral matter, amino acid, salt and sugar are weighed respectively by weight, then by the composite mineral matter, institute It states amino acid, the salt and the sugar to be separately added into the mixture I that step (1) obtains, and stirs so that above-mentioned substance is mixed It closes uniformly, obtains mixture II;
(3) leavening is weighed by weight, and weighs the sterile water of 25 times of leavenings by weight, then by the fermentation Agent is added in above-mentioned sterile water, is sufficiently stirred so that leavening is uniformly mixed with sterile water, obtains diluting bacterium solution;
(4) dilution bacterium solution obtained in step (3) is added in the obtained mixture II of step (2), is sufficiently stirred So that the dilution bacterium solution is uniformly mixed with the mixture II, mixture III is obtained;Then mixture III is compacted, then used Plastic film is placed in container after being sealed, the time for making mixture III ferment under conditions of 20 DEG C of temperature of fermentation For 35 days to get ensilage.
Embodiment 2
Paper mulberry ensilage provided by the invention is prepared by formula as below and method:
A kind of paper mulberry ensilage, is mainly made of raw material from the following weight: 70 parts of feed with paper-mulberry leaf, 30 parts of corn stover, Osmanthus No. 1 20 parts of imperatoria of grass, 23 parts of feed mulberry leaf, 8 parts of Canterburybells, 3 parts of composite mineral matter .5 parts of amino acid 1,1 part of salt, sugar 8 Part and 2 parts of leavening;The leavening is the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and ball hair The mixture of shell bacterium, wherein the green sugar sporangium, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and chaetomium globosum Weight part ratio be 2.5:2.5:5:3:2.Wherein, the composite mineral matter is calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium Mixture, the calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium are with 2:2:2:0.6:0.5:0.5:0.4:0.4:0.3: 0.3 weight ratio mixes;The amino acid is the mixture of tryptophan, lysine, histidine and threonine, the color ammonia Acid, lysine, histidine and threonine are mixed with the weight ratio of 2:2:1.5:1.
Paper mulberry ensilage the preparation method is as follows:
(1) feed with paper-mulberry leaf, corn stover, osmanthus careless No. 1 imperatoria, feed mulberry leaf, Canterburybells are weighed respectively by weight, it will be above-mentioned It is 66% that raw material, which are cleaned respectively and are dried to moisture content, then by the feed with paper-mulberry leaf after drying, corn stover, careless No. 1 imperatoria in osmanthus, Feed mulberry leaf and Canterburybells are switched to broken section that length is 1.5cm respectively, respectively obtain broken section of feed with paper-mulberry leaf, broken section of corn stover, osmanthus Careless No. 1 broken section of imperatoria, broken section of feed mulberry leaf and broken section of Canterburybells, broken section of the above raw material is mixed, mixture I is obtained;
(2) composite mineral matter, amino acid, salt and sugar are weighed respectively by weight, then by the composite mineral matter, institute It states amino acid, the salt and the sugar to be separately added into the mixture I that step (1) obtains, and stirs so that above-mentioned substance is mixed It closes uniformly, obtains mixture II;
(3) leavening is weighed by weight, and weighs the sterile water of 28 times of leavenings by weight, then by the fermentation Agent is added in above-mentioned sterile water, is sufficiently stirred so that leavening is uniformly mixed with sterile water, obtains diluting bacterium solution;
(4) dilution bacterium solution obtained in step (3) is added in the obtained mixture II of step (2), is sufficiently stirred So that the dilution bacterium solution is uniformly mixed with the mixture II, mixture III is obtained;Then mixture III is compacted, then used Plastic film is placed in container after being sealed, the time for making mixture III ferment under conditions of 28 DEG C of temperature of fermentation For 40 days to get ensilage.
Embodiment 3
Paper mulberry ensilage provided by the invention is prepared by formula as below and method:
A kind of paper mulberry ensilage, is mainly made of raw material from the following weight: 75 parts of feed with paper-mulberry leaf, 35 parts of corn stover, Osmanthus No. 1 25 parts of imperatoria of grass, 25 parts of feed mulberry leaf, 10 parts of Canterburybells, 4 parts of composite mineral matter, 2 parts of amino acid, 1.5 parts of salt, sugar 10 parts and 3 parts of leavening;The leavening is the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and ball The mixture of cupreum, wherein the green sugar sporangium, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and ball hair shell The weight part ratio of bacterium is 3:3:6:4:3.Wherein, the composite mineral matter is calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium Mixture, the calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium are with 2:2:2:0.6:0.5:0.5:0.4:0.4:0.3:0.3 Weight ratio mix;The amino acid be tryptophan, lysine, histidine and threonine mixture, the tryptophan, Lysine, histidine and threonine are mixed with the weight ratio of 2:2:1.5:1.
Paper mulberry ensilage the preparation method is as follows:
(1) feed with paper-mulberry leaf, corn stover, careless No. 1 imperatoria in osmanthus, feed mulberry leaf and Canterburybells are weighed respectively by weight, it will be upper Stating raw material and cleaning respectively and be dried to moisture content is 75%, then by the feed with paper-mulberry leaf after drying, corn stover, careless No. 1 king in osmanthus Grass, feed mulberry leaf and Canterburybells are switched to broken section that length is 2cm respectively, respectively obtain broken section of feed with paper-mulberry leaf, broken section of corn stover, osmanthus Careless No. 1 broken section of imperatoria, broken section of feed mulberry leaf and broken section of Canterburybells, broken section of the above raw material is mixed, mixture I is obtained;
(2) composite mineral matter, amino acid, salt and sugar are weighed respectively by weight, then by the composite mineral matter, institute It states amino acid, the salt and the sugar to be separately added into the mixture I that step (1) obtains, and stirs so that above-mentioned substance is mixed It closes uniformly, obtains mixture II;
(3) leavening is weighed by weight, and weighs the sterile water of 30 times of leavenings by weight, then by the fermentation Agent is added in above-mentioned sterile water, is sufficiently stirred so that leavening is uniformly mixed with sterile water, obtains diluting bacterium solution;
(4) dilution bacterium solution obtained in step (3) is added in the obtained mixture II of step (2), is sufficiently stirred So that the dilution bacterium solution is uniformly mixed with the mixture II, mixture III is obtained;Then mixture III is compacted, then used Plastic film is placed in container after being sealed, the time for making mixture III ferment under conditions of 35 DEG C of temperature of fermentation For 45 days to get ensilage.
Embodiment 4
Paper mulberry ensilage provided by the invention is prepared by formula as below and method:
A kind of paper mulberry ensilage, without the sugared sporangium of green, Neurospora sitophila and Clostridium thermocellum in leavening, in Raw material and preparation method are same as Example 2.
Embodiment 5
Paper mulberry ensilage provided by the invention is prepared by formula as below and method:
A kind of paper mulberry ensilage is free of Melon fusarium Wilt and chaetomium globosum in leavening, in raw material and Preparation method is same as Example 2.
Compliance test result
The application value of paper mulberry ensilage in order to further illustrate the present invention, after finished product is made in embodiment 1-5 Feeding trial is carried out to goat.
(1) compliance test result of palatability, digestibility and strengthen immunity effect
Test goat object: the good little goat of 18 big health status of 50 ages in days is selected as test sheep, gender Ratio is 1:1, and the figure of these little goats is suitable, weight is suitable, these little goats are randomly divided into 6 groups, each group 3, often Battery of tests little goat is continuously fed 30 days with corresponding feed, and each test little goat cannot contact during guarantee test Other foods.
Palatability verification method: being with reference to the evaluation point as feed palatability, by each examination using the grazing rate of little goat The grazing rate for testing group little goat is reported in Table 1 below, specifically: a food basin is provided for each test group, daily feeding 3 is eaten, often Meal feeds identical amount, and record provides the total amount of feed every day, and the feed for re-recording each food basin before the meal by second day first is surplus Surplus, calculates the grazing rate of each test group little goat, then calculates the average daily grazing rate of each test group little goat, and will be each The average daily grazing rate of test group little goat is reported in Table 1 below.Wherein, the calculation of grazing rate are as follows: grazing rate=(provide feed Total amount-feed surplus)/provide feed total amount * 100%.
Digest and assimilate situation and immunity situation verification method: using the average weight increment of little goat be with reference to as The evaluation point that feed digestion absorbs is with reference to the evaluation point as immunity situation, wherein 5 examinations using the health status of little goat It tests group finished product made of embodiment 1-5 to be fed, another test group is fed with commercially available Silage Corn Straw, often One group is continuously fed 30 days with corresponding feed, measures each test group little goat being averaged after continuous feed 30 days respectively Weight, counts the average weight increment of each test group little goat, and is reported in Table 1 below respectively, observes during test Each test group tests the health condition of little goat, evaluates the health condition of each test group lamb, comments respectively Price card is quasi- are as follows: little goat do not occur disease condition be chosen as it is first-class;Little goat occurs wherein the one of mild diarrhea, lassitude etc. Kind of illness is chosen as the second grade, and what the two or more illnesss such as diarrhea, lassitude occurred in little goat is chosen as the third gradegrade C, by the above evaluation mark Standard evaluates the health status of the test little goat of each test group, and is reported in Table 1 below respectively.Test result such as the following table 1 It is shown:
Table 1
Test group The feed of nursing Average daily grazing rate Average weight increment Health status grade
Test group 1 1 finished product of embodiment 95% 1430g It is first-class
Test group 2 2 finished product of embodiment 96% 1421g It is first-class
Test group 3 3 finished product of embodiment 94% 1395g It is first-class
Test group 4 4 finished product of embodiment 74% 1038g It is first-class
Test group 5 5 finished product of embodiment 93% 1390g The second grade
Control group Commercially available Silage Corn Straw 75% 1042g The second grade
In upper table, embodiment 1-3 finished product is ensilage of the present invention, and embodiment 4-5 finished product is made of comparative example Finished product, control group finished product is commercially available Silage Corn Straw, wherein without the sugared sporangium of green, good in the leavening of embodiment 4 Eat neurospora and Clostridium thermocellum;Melon fusarium Wilt and chaetomium globosum are free of in the leavening of embodiment 5;And in this test, Xylo-oligosaccharide or oligofructose are not contained in commercially available Silage Corn Straw finished product used in control group.
By upper table data it is found that the average daily grazing rate of test group 1-3, average weight increment are obviously higher than other tests Group, health status is good, and the average daily grazing rate of control group, average weight increment and health status can not show a candle to use The test group that finished product of the present invention is fed, it was demonstrated that progress place of the invention.The data of comparative test group 4 and test group 2, it will be seen that examination The average daily grazing rate and average body weight increase amount for testing group 4 are not so good as test group 2, it was demonstrated that green sugar sporangium, Neurospora sitophila and The addition of Clostridium thermocellum has digestion and absorption effect of the enhancing goat to feed, while improving the palatability of feed;To having a competition Test the data of group 5 and test group 2, it was demonstrated that the addition of Melon fusarium Wilt and chaetomium globosum is so that raw material pass through series reaction The beneficial agents of goat immune power can be improved to generate sufficient amount.The present invention demonstrated above, which has, improves palatability, digestion suction The effect of yield and strengthen immunity.
(2) it is proliferated intestinal bifidobacteria compliance test result
Select the good little goat of 18 big health status of 50 ages in days as test sheep, sex ratio 1:1, these are small The figure of goat is suitable, weight is suitable, and test little goat is randomly divided into 6 groups, and every group 3.Wherein 5 test groups are implemented Example 1-5 made of finished feed little goat is fed, wherein one group as a control group, with commercially available Silage Corn Straw pair Little goat is fed, continuous to feed 14 days, and every meal supplies each test group little goat the feed of same amount daily, and guarantees to try Each test little goat cannot contact other foods during testing, and before giving feed and give feed last time for 24 hours Afterwards, the excrement 0.1-0.4g of each test group little goat of aseptic collection, and the quantity of each test group Bifidobacterium is measured, It is reported in Table 2 below respectively.Test result is as shown in table 2 below:
Table 2
Test group The feed of nursing Before nursing (log cfu/g) After feeding 14 days (log cfu/g)
Test group 1 1 finished product of embodiment 7.11 7.56
Test group 2 2 finished product of embodiment 7.13 7.54
Test group 3 3 finished product of embodiment 7.08 7.48
Test group 4 4 finished product of embodiment 7.20 7.62
Test group 5 5 finished product of embodiment 7.11 7.15
Control group Commercially available Silage Corn Straw 7.18 7.16
As seen from the above table, the quantity of Bifidobacterium of the test group 1-4 before and after with corresponding forage feed in excrement has Different degrees of increases, and the data of comparative test group 5 and test group 2 and control group respectively, it was demonstrated that muskmelon is withered in leavening The addition of bacillus or chaetomium globosum, which can promote raw material to generate Bifidobacterium in proliferation enteron aisle by a series of reaction, to be had The beneficial factor.The present invention demonstrated above has the function of being proliferated intestinal bifidobacteria.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, fall within the scope of the claimed invention without departing from theon the basis of the spirit of the present invention.

Claims (7)

1. a kind of paper mulberry ensilage, which is characterized in that be mainly made of raw material from the following weight: 60-75 parts of feed with paper-mulberry leaf, jade 20-35 parts of stalk of rice, osmanthus careless No. 1 18-25 parts of imperatoria, 20-25 parts of feed mulberry leaf, 6-10 parts of Canterburybells, composite mineral matter 2-4 Part, -2 parts of amino acid 1,0.8-1.5 parts of salt, 6-10 parts of sugar and 1-3 parts of leavening;The leavening be the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and the mixture of chaetomium globosum, wherein green sugared sporangium, the well food Neurospora, Clostridium thermocellum, Melon fusarium Wilt and chaetomium globosum weight part ratio be 2-3:2-3:3-6:2-4:1-3.
2. a kind of paper mulberry ensilage according to claim 1, which is characterized in that mainly by the raw material system of following parts by weight At: 70 parts of feed with paper-mulberry leaf, 30 parts of corn stover, osmanthus careless No. 1 20 parts of imperatoria, 23 parts of feed mulberry leaf, 8 parts of Canterburybells, composite mineral matter 3 Part .5 parts of amino acid 1,1 part of salt, 8 parts and 2 parts of leavening of sugar;The leavening be the sugared sporangium of green, Neurospora sitophila, Clostridium thermocellum, Melon fusarium Wilt and the mixture of chaetomium globosum, wherein the green sugar sporangium, Neurospora sitophila, heat are fine The weight part ratio of clostridium, Melon fusarium Wilt and chaetomium globosum is 2.5:2.5:5:3:2.
3. a kind of paper mulberry ensilage according to claim 1 or 2, it is characterised in that: the composite mineral matter be calcium, Phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium mixture, the calcium, phosphorus, sodium, copper, iron, silicon, manganese, cobalt, iodine and selenium are with 2:2: 2:0.6:0.5:0.5:0.4:0.4:0.3:0.3 weight ratio mix.
4. a kind of paper mulberry ensilage according to claim 1 or 2, it is characterised in that: the amino acid is tryptophan, relies The mixture of propylhomoserin, histidine and threonine, the tryptophan, lysine, histidine and threonine are with the weight of 2:2:1.5:1 Than mixing.
5. a kind of method for preparing paper mulberry ensilage of any of claims 1 or 2, which comprises the following steps:
(1) feed with paper-mulberry leaf, corn stover, careless No. 1 imperatoria in osmanthus, feed mulberry leaf and Canterburybells are weighed respectively by weight, by above-mentioned original It is 55-75% that material, which is cleaned respectively and is dried to moisture content, then by the feed with paper-mulberry leaf after drying, corn stover, careless No. 1 king in osmanthus Grass, feed mulberry leaf and Canterburybells are switched to broken section that length is 1-2cm respectively, respectively obtain broken section of feed with paper-mulberry leaf, broken section of corn stover, Osmanthus No. 1 broken section of imperatoria of grass, broken section of feed mulberry leaf and broken section of Canterburybells, broken section of the above raw material is mixed, mixture I is obtained;
(2) composite mineral matter, amino acid, salt and sugar are weighed respectively by weight, then by the composite mineral matter, the ammonia Base acid, the salt and the sugar are separately added into the mixture I that step (1) obtains, and are stirred so that above-mentioned substance mixing is equal It is even, obtain mixture II;
(3) leavening is weighed by weight, and weighs the water of 25-30 times of leavening by weight, then adds the leavening Enter in above-mentioned water, be sufficiently stirred so that leavening is uniformly mixed with water, obtains dilution bacterium solution;
(4) will obtained in step (3) dilution bacterium solution be added in the obtained mixture II of step (2), be sufficiently stirred so that The dilution bacterium solution is uniformly mixed with the mixture II, obtains mixture III;Then mixture III is compacted, then uses plastics It is placed in container and ferments after diaphragm seal is good, the time of fermentation is 35-45 days to get ensilage.
6. a kind of method for preparing paper mulberry ensilage according to claim 5, it is characterised in that: the water in step (2) For sterile water.
7. a kind of method for preparing paper mulberry ensilage according to claim 5, it is characterised in that: fermentation in step (4) Temperature be 20-35 DEG C.
CN201810874144.8A 2018-08-03 2018-08-03 A kind of paper mulberry ensilage and preparation method thereof Pending CN109007263A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113229409A (en) * 2021-05-27 2021-08-10 山西省农业科学院饲料兽药研究所 Preparation process of paper mulberry silage
CN114916619A (en) * 2022-03-31 2022-08-19 广西大学 Concentrated feed for goats in gestation period and preparation method and application thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106173362A (en) * 2016-07-29 2016-12-07 广西壮族自治区农业科学院农产品加工研究所 The method producing cattle and sheep complete feed for raw material ferment in second time with Broussonetia papyrifera branch and leaf
CN108041289A (en) * 2017-12-15 2018-05-18 湖北省农业科学院畜牧兽医研究所 A kind of paper mulberry special ensiling additive and application

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106173362A (en) * 2016-07-29 2016-12-07 广西壮族自治区农业科学院农产品加工研究所 The method producing cattle and sheep complete feed for raw material ferment in second time with Broussonetia papyrifera branch and leaf
CN108041289A (en) * 2017-12-15 2018-05-18 湖北省农业科学院畜牧兽医研究所 A kind of paper mulberry special ensiling additive and application

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
丁梦璇等: "绿色糖单孢菌木质素过氧化物酶的分离纯化及鉴定", 《生物技术通报》 *
官敏: "好食脉孢菌(N.sitophila)产锰过氧化物酶培养条件的优化、酶的纯化以及酶学性质的分析", 《中国优秀博硕士学位论文全文数据库(硕士)基础科学辑》 *
胡晓瑜: "酶法生产低聚木糖的研究", 《中国优秀硕士学位论文全文数据库 基础科学辑》 *
陈林等: "热纤梭菌高效降解木质纤维素过程的组学研究进展", 《微生物学报》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113229409A (en) * 2021-05-27 2021-08-10 山西省农业科学院饲料兽药研究所 Preparation process of paper mulberry silage
CN114916619A (en) * 2022-03-31 2022-08-19 广西大学 Concentrated feed for goats in gestation period and preparation method and application thereof

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