CN109005984A - A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection - Google Patents
A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection Download PDFInfo
- Publication number
- CN109005984A CN109005984A CN201811111955.9A CN201811111955A CN109005984A CN 109005984 A CN109005984 A CN 109005984A CN 201811111955 A CN201811111955 A CN 201811111955A CN 109005984 A CN109005984 A CN 109005984A
- Authority
- CN
- China
- Prior art keywords
- growth
- grafting
- sprout
- agent
- oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D1/00—Fertilisers containing potassium
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to a kind of oil-tea sprout with grafting to survive agent and preparation method thereof with environmental protection, belongs to plant growth preparation technical field.The present invention promotes garden seedling tree quickly to breed by the auximone extracted in growth period sorghum fringe, and nursery stock is made to keep excellent growth conditions, which adds utility ratio of cuttage technique during nursery stock production, the gluing liquid cladding grafting wound being made of simultaneously chitosan and starch aquation, the overlap joint channel of fixed sprout and wound, and there is callus effect, and as carrier loaded plant ash, potassium permanganate is as sprout nutrient solution, the nutrient of growth required consumption is provided for sprout, and chitosan also has antibacterial action, resist injury of the external germ to sprout growth survival in sprout growth course, improve grafting survival performance.
Description
Technical field
The present invention relates to a kind of oil-tea sprout with grafting to survive agent and preparation method thereof with environmental protection, belongs to plant growth preparation skill
Art field.
Background technique
Oil tea is primarily referred to as a kind of plant that is higher containing grease in Theaceae Camellia Plants and having cultivation economic value
General name, be the important woody oleiferous plants crop in China.Tea-seed oil and its byproduct all have very high use value and advanced
Vegetable edible oil has nutrition, medical treatment, health care, beauty and other effects.Meanwhile oil tea impoverishment tolerant ability is strong, does not strive ground with crop,
Have both conserve water and soil, a variety of ecological benefits such as water conservation.Oil tea is resourceful, and cultivation history is long, and wherein mountain shaddock tea produces
Tea-seed oil has the mouthfeel and quality different from interior ground tea-seed oil, and price is 10 times or so of interior ground tea seed oil price lattice.Oil tea is different
Pollinated plant is spent, is bred between plant by natural hybrization, characters of progenies differentiation is larger, and individual plants are very different, sexual propagation
The economic characters separation of offspring is obvious, using vegetative propagations such as grafting and cuttages, is able to maintain the high yield of elite stand, stable yields, resists
The good characteristics such as disease, thus produce and the breeding of Nurse seed grafting or cuttage progress high-yield tea-oil breeding and popularization is mainly taken to answer
With.
Propagation by grafiting, which has, to keep good characteristic, enhancing plant resistance and adaptability, expanding propagation coefficient, changes plant sight
The advantages that appreciating form can be used as the important means of new varieties initial stage breeding.Both at home and abroad about plant graft fast breeding technique study compared with
It is more, mainly pass through the influence of Orthogonal Experiment and Design or randomized block experiment design search various factors to graft survival rate.It selects
Excellent scion and stock is grafted, and plant resistance and disease resistance can be enhanced, and promotes vine growth and development, improves yield.
But oil-tea sprout anvil graft technology requires high, seasonal strong, production Sheng Bengao low compared with cutting propagation coefficient；And it adopts
With cottage method, technical requirements are fairly simple, and production cost is low, but there is the problems such as slow, root system is undeveloped that take root.Therefore, have
Necessity carries out a series of careful deep oil tea cuttage researchs.
Summary of the invention
The technical problems to be solved by the invention: requiring height for existing oil-tea sprout anvil graft technology, seasonal strong, compared with
Cutting propagation coefficient is low, produces Sheng Bengao, and uses cottage method, and technical requirements are fairly simple, and production cost is low, but there is life
Root is slow, the underdeveloped problem of root system, provides a kind of oil-tea sprout with grafting with environmental protection and survives agent and preparation method thereof.
In order to solve the above technical problems, the technical solution adopted by the present invention is that:
A kind of oil-tea sprout with grafting survives agent with environmental protection, described to survive agent and be made of growth-promoting media and nutrient solution, the growth-promoting media with
The mass ratio of nutrient solution is 1:2~1:5.
The growth-promoting media is the ethanol solution extracting solution of sorghum fringe.
The sorghum fringe that the sorghum fringe is growth period 2~3 months, ground 40 mesh are made.
The mass fraction of the ethanol solution is 50%, and the mass ratio of sorghum fringe and ethanol solution is 1:3~1:10.
The nutrient solution is that 2% acetum, starch, plant ash, potassium permanganate form by chitosan, mass fraction.
Nutrient solution composition parts by weight are 2~3 parts of chitosans, and 300~400 parts of mass fractions are 2% acetum, 40
~80 parts of starch, 10~20 parts of plant ash, 3.0~4.5 parts of potassium permanganate.
A kind of oil-tea sprout with grafting environmental protection survives the preparation method of agent, specific steps are as follows:
(1) it is ground after taking growth period 2~3 months sorghum fringes, washing to air-dry, crosses 40 meshes, obtain sorghum fringe particle；
(2) take sorghum fringe particle be added mass fraction be 50% ethanol solution in, after mixing heating stirring to 50~60 DEG C protect
Temperature 2~6h of stirring, then be centrifugated and take supernatant, supernatant is dried at 80~90 DEG C to the 40~50% of stoste volume, is obtained
Growth-promoting media；
(3) taking chitosan that mass fraction is added is to be stirring evenly and then adding into starch in 2% acetum, is kept the temperature at 60~80 DEG C
1~2h is stirred, plant ash, potassium permanganate are added after being cooled to room temperature, stirs evenly and filters removal solid particle, obtain nutrition
Liquid；
(4) 1:2~1:5 in mass ratio mixes growth-promoting media with nutrient solution, obtains oil-tea sprout with grafting with environmental protection and survives agent.
The present invention is compared with other methods, and advantageous effects are:
(1) present invention promotes garden seedling tree quickly to breed by the auximone extracted in growth period sorghum fringe, and makes
Nursery stock keeps excellent growth conditions, and which adds utility ratio of cuttage technique during nursery stock production, while by shell
The gluing liquid cladding grafting wound of glycan and starch aquation composition, the overlap joint channel of fixed sprout and wound, and there is callus to make
With, and the nutrition member of growth required consumption is provided for sprout as sprout nutrient solution as carrier loaded plant ash, potassium permanganate
Element, and chitosan also has antibacterial action, resists injury of the external germ to sprout growth survival in sprout growth course, mentions
High grafting survival performance；
(2) oil-tea sprout with grafting prepared by the present invention, which survives agent with environmental protection, can promote the rapid flourish of sprout, and lose in parent
Aspect better effect is passed, in addition, the period of the mode of cultivation is shorter, and biggish investment will not be spent, the differentiation of nursery stock callus,
Inducing effect is good, is worth of widely use.
Specific embodiment
Growth period 2~3 months sorghum fringes, natural air drying after being washed with deionized 2~3 times are taken, then is transferred to and is ground
1~2h is ground in grinding machine, 40 meshes is crossed, obtains sorghum fringe particle, takes 300~500g sorghum fringe particle, and 1.5~3.0kg matter is added
Measuring score is heating stirring to 50~60 DEG C of 2~6h of insulated and stirred after mixing, after to be mixed in 50% ethanol solution
It is cooled to room temperature and is transferred in centrifuge and be centrifugated, 10~30min is centrifugated with 3000~4000r/min, takes supernatant,
Supernatant is placed in drying box, is dried at 80~90 DEG C to the 40~50% of stoste volume, is obtained growth-promoting media, take 2~3g shell poly-
Sugar, be added 300~400g mass fraction be 2% acetum in, with 300~400r/min stir 20~30min after be added 40~
10~20g plant ash, 3.0~4.5g Gao Meng is added in 80g starch, 1~2h of insulated and stirred at 60~80 DEG C after being cooled to room temperature
Sour potassium continues filtering removal solid particle after 30~40min of stirring, obtains nutrient solution, 1:2~1:5 in mass ratio by growth-promoting media with
Nutrient solution mixing, obtains oil-tea sprout with grafting with environmental protection and survives agent.
Example 1
Growth period 2 months sorghum fringes, natural air drying after being washed with deionized 2 times are taken, then is transferred in grinder and is ground
1h crosses 40 meshes, obtains sorghum fringe particle, takes 300g sorghum fringe particle, and it is to mix in 50% ethanol solution that 1.5kg mass fraction, which is added,
Heating stirring is cooled to room temperature after to be mixed to 50 DEG C of insulated and stirred 2h and is transferred to centrifugation point in centrifuge after closing uniformly
From, 10min is centrifugated with 3000r/min, supernatant is taken, supernatant is placed in drying box, it is dry to stoste at 80 DEG C
The 40% of volume, obtains growth-promoting media, takes 2g chitosan, and it is to be stirred in 2% acetum with 300r/min that 300g mass fraction, which is added,
Addition 40g starch after 20min, the insulated and stirred 1h at 60 DEG C, addition 10g plant ash after being cooled to room temperature, 3.0g potassium permanganate,
Continue filtering after stirring 30min and remove solid particle, obtains nutrient solution, 1:2 in mass ratio mixes growth-promoting media with nutrient solution, obtains oily
Tea sprout grafting survives agent with environmental protection.
Example 2
Growth period 3 months sorghum fringes, natural air drying after being washed with deionized 3 times are taken, then is transferred in grinder and is ground
1h crosses 40 meshes, obtains sorghum fringe particle, takes 400g sorghum fringe particle, and it is to mix in 50% ethanol solution that 2.0kg mass fraction, which is added,
Heating stirring is cooled to room temperature after to be mixed to 55 DEG C of insulated and stirred 4h and is transferred to centrifugation point in centrifuge after closing uniformly
From, 15min is centrifugated with 3000r/min, supernatant is taken, supernatant is placed in drying box, it is dry to stoste at 85 DEG C
The 45% of volume, obtains growth-promoting media, takes 2g chitosan, and it is to be stirred in 2% acetum with 350r/min that 350g mass fraction, which is added,
Addition 60g starch after 25min, the insulated and stirred 1h at 70 DEG C, addition 15g plant ash after being cooled to room temperature, 3.6g potassium permanganate,
Continue filtering after stirring 35min and remove solid particle, obtains nutrient solution, 1:3 in mass ratio mixes growth-promoting media with nutrient solution, obtains oily
Tea sprout grafting survives agent with environmental protection.
Example 3
Growth period 3 months sorghum fringes, natural air drying after being washed with deionized 3 times are taken, then is transferred in grinder and is ground
2h crosses 40 meshes, obtains sorghum fringe particle, takes 500g sorghum fringe particle, and it is to mix in 50% ethanol solution that 3.0kg mass fraction, which is added,
Heating stirring is cooled to room temperature after to be mixed to 60 DEG C of insulated and stirred 6h and is transferred to centrifugation point in centrifuge after closing uniformly
From, 30min is centrifugated with 4000r/min, supernatant is taken, supernatant is placed in drying box, it is dry to stoste at 90 DEG C
The 50% of volume, obtains growth-promoting media, takes 3g chitosan, and it is to be stirred in 2% acetum with 400r/min that 400g mass fraction, which is added,
Addition 80g starch after 30min, the insulated and stirred 2h at 80 DEG C, addition 20g plant ash after being cooled to room temperature, 4.5g potassium permanganate,
Continue filtering after stirring 40min and remove solid particle, obtains nutrient solution, 1:5 in mass ratio mixes growth-promoting media with nutrient solution, obtains oily
Tea sprout grafting survives agent with environmental protection.
Reference examples: the environmental protection of Nanjing company production survives agent.
It environmental protection is prepared in example and reference examples survives agent and detect, specific test result such as table 1.
1 performance characterization contrast table of table
| Detection project | Example 1 | Example 2 | Example 3 | Reference examples |
| Survival rate/% | 75.25 | 78.75 | 81.34 | 62.50 |
| Callus rate/% | 57.08 | 61.14 | 64.23 | 45.89 |
| Rooting rate/% | 61.1 | 63.2 | 69.3 | 52.1 |
As shown in Table 1, oil-tea sprout with grafting prepared by the present invention, which survives agent with environmental protection, can promote the rapid flourish of sprout, can make
The differentiation of nursery stock callus, inducing effect are good, are worth of widely use.
Claims (7)
1. a kind of oil-tea sprout with grafting survives agent with environmental protection, which is characterized in that it is described to survive agent and be made of growth-promoting media and nutrient solution,
The mass ratio of the growth-promoting media and nutrient solution is 1:2~1:5.
2. a kind of oil-tea sprout with grafting as described in claim 1 survives agent with environmental protection, which is characterized in that the growth-promoting media is height
The ethanol solution extracting solution of fine strain of millet fringe.
3. a kind of oil-tea sprout with grafting as claimed in claim 2 survives agent with environmental protection, which is characterized in that the sorghum fringe is made a living
Long-term 2~3 months sorghum fringes, ground 40 mesh are made.
4. a kind of oil-tea sprout with grafting as claimed in claim 2 survives agent with environmental protection, which is characterized in that the ethanol solution
Mass fraction is 50%, and the mass ratio of sorghum fringe and ethanol solution is 1:3~1:10.
5. a kind of oil-tea sprout with grafting as described in claim 1 survives agent with environmental protection, which is characterized in that the nutrient solution is by shell
Glycan, mass fraction are 2% acetum, starch, plant ash, potassium permanganate composition.
6. a kind of oil-tea sprout with grafting as claimed in claim 5 survives agent with environmental protection, which is characterized in that the nutrient solution composition
Parts by weight are 2~3 parts of chitosans, and 300~400 parts of mass fractions are 2% acetum, 40~80 parts of starch, 10~20 portions of vegetations
Ash, 3.0~4.5 parts of potassium permanganate.
7. a kind of oil-tea sprout with grafting environmental protection as described in claim 1~6 any one survives the preparation method of agent, special
Sign is, specific steps are as follows:
(1) it is ground after taking growth period 2~3 months sorghum fringes, washing to air-dry, crosses 40 meshes, obtain sorghum fringe particle；
(2) take sorghum fringe particle be added mass fraction be 50% ethanol solution in, after mixing heating stirring to 50~60 DEG C protect
Temperature 2~6h of stirring, then be centrifugated and take supernatant, supernatant is dried at 80~90 DEG C to the 40~50% of stoste volume, is obtained
Growth-promoting media；
(3) taking chitosan that mass fraction is added is to be stirring evenly and then adding into starch in 2% acetum, is kept the temperature at 60~80 DEG C
1~2h is stirred, plant ash, potassium permanganate are added after being cooled to room temperature, stirs evenly and filters removal solid particle, obtain nutrition
Liquid；
(4) 1:2~1:5 in mass ratio mixes growth-promoting media with nutrient solution, obtains oil-tea sprout with grafting with environmental protection and survives agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811111955.9A CN109005984A (en) | 2018-09-25 | 2018-09-25 | A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811111955.9A CN109005984A (en) | 2018-09-25 | 2018-09-25 | A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109005984A true CN109005984A (en) | 2018-12-18 |
Family
ID=64618201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811111955.9A Pending CN109005984A (en) | 2018-09-25 | 2018-09-25 | A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109005984A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055859A (en) * | 1990-04-21 | 1991-11-06 | 冯成汉 | A kind of production method of phytohormone emulsion |
| CN103739402A (en) * | 2014-01-21 | 2014-04-23 | 中南林业科技大学 | Healing agent for jujube grafting and application method thereof |
| CN104350914A (en) * | 2014-10-24 | 2015-02-18 | 定西市农业科学研究院 | Early-spring transplantation and seedling protection method for facility-grafted jujube seedlings |
| CN105454326A (en) * | 2014-10-01 | 2016-04-06 | 饶明高 | Preparation method of selenium-rich sorghum auxin |
| CN105557432A (en) * | 2016-01-07 | 2016-05-11 | 郎溪县姚村乡盛茂贵茶叶种植家庭农场 | Cuttage seedling raising method for promoting quick growth of yellow tea |
-
2018
- 2018-09-25 CN CN201811111955.9A patent/CN109005984A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1055859A (en) * | 1990-04-21 | 1991-11-06 | 冯成汉 | A kind of production method of phytohormone emulsion |
| CN103739402A (en) * | 2014-01-21 | 2014-04-23 | 中南林业科技大学 | Healing agent for jujube grafting and application method thereof |
| CN105454326A (en) * | 2014-10-01 | 2016-04-06 | 饶明高 | Preparation method of selenium-rich sorghum auxin |
| CN104350914A (en) * | 2014-10-24 | 2015-02-18 | 定西市农业科学研究院 | Early-spring transplantation and seedling protection method for facility-grafted jujube seedlings |
| CN105557432A (en) * | 2016-01-07 | 2016-05-11 | 郎溪县姚村乡盛茂贵茶叶种植家庭农场 | Cuttage seedling raising method for promoting quick growth of yellow tea |
Non-Patent Citations (1)
| Title |
|---|
| 贾鸿玉: "《绿色通道·高三生物》", 30 April 2008 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102487627B (en) | Method for effectively breaking dormancy of Paris polyphylla seeds | |
| CN102030581B (en) | Novel multi-functional biological organic seed dressing agent and method for preparing same | |
| CN106717824A (en) | One method for culturing seedlings for growing cotton | |
| CN102823360B (en) | Method for releasing dormancy of Canadian redbud seeds | |
| CN104982217A (en) | Paris polyphylla seed propagating method | |
| CN109220790B (en) | In vitro propagation method of rhododendron simsii | |
| CN102191214B (en) | Preparation method for resveratrol-producing cell and special-purpose suspension medium thereof | |
| CN104137676B (en) | A kind of raw Stipa seeds of graminous pasture sand takes off awns and cleans method | |
| CN105009989A (en) | Macadamia nut seedling cultivation substrate prepared through macadamia nut waste | |
| CN107183080A (en) | A kind of flowers cuttage root-taking agent and preparation method thereof | |
| CN105819983A (en) | Flowering plant coated fertilizer capable of prolonging flowering phase | |
| CN106561115B (en) | Method for promoting germination of suaeda salsa seeds | |
| CN104230404B (en) | A kind of preparation method for digesting high activity seaweed organic fertilizer material and products thereof | |
| CN105917792B (en) | A method of improving paris polyphylla seeding ratio | |
| CN104782361A (en) | Bletilla striata planting method | |
| CN109005984A (en) | A kind of oil-tea sprout with grafting survives agent and preparation method thereof with environmental protection | |
| CN106995347A (en) | A kind of Flower Plants in Hydroponic Culture nutrient solution and preparation method thereof | |
| CN206993640U (en) | A kind of multifunctional agriculture incubator | |
| CN101381256A (en) | Anthurium andreanum nutrient fluid | |
| CN110050541A (en) | A kind of method of Chinese tallow tree seed fast dewaxing vernalization | |
| CN114208848B (en) | Corn growth regulator containing chitosan and preparation and use methods thereof | |
| CN109874667A (en) | A kind of breeding method of Pelargonium roseum polyploid | |
| CN101444156A (en) | Seed treating method used in culture of idesia polycarpa maxim. var. vestita diels | |
| CN108040845A (en) | A kind of method for culturing seedlings of oil Paeonia suffruticosa seed | |
| CN107278426A (en) | A kind of multifunctional agriculture incubator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20181218 |