CN106561115B - Method for promoting germination of suaeda salsa seeds - Google Patents
Method for promoting germination of suaeda salsa seeds Download PDFInfo
- Publication number
- CN106561115B CN106561115B CN201611006226.8A CN201611006226A CN106561115B CN 106561115 B CN106561115 B CN 106561115B CN 201611006226 A CN201611006226 A CN 201611006226A CN 106561115 B CN106561115 B CN 106561115B
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- seeds
- germination
- suaeda
- suaeda salsa
- potassium permanganate
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
Abstract
The invention discloses a method for promoting the germination of suaeda salsa seeds, which comprises the following steps: selecting Suaeda salsa seeds by a flotation method, soaking the selected Suaeda salsa seeds in a 1% potassium permanganate solution for 8 hours, placing the Suaeda salsa seeds in a culture dish padded with double-layer filter paper, and culturing the Suaeda salsa seeds in distilled water at 25 ℃ to obtain germinated seeds. The method for promoting the germination of the suaeda salsa seeds, which is provided by the invention, has the advantages that the technical operation is simple, the problem of low germination rate of the suaeda salsa seeds is solved, the germination rate of the seeds is high, and the germination rate is improved to more than 88%.
Description
Technical Field
The invention relates to the technical field of plant planting, in particular to a method for promoting the germination of suaeda salsa seeds.
Background
Suaeda salsa (Suaeda salsa), an annual herbaceous plant, is generally grown in saline-alkali soil, seaside and lake, is a typical saline-alkali soil indicator plant and is distributed in europe, asia and china in northeast, inner Mongolia, Xinjiang, Gansu, Shanxi, Hebei, Shandong, Zhejiang and the like. The stems and leaves of the suaeda glauca seedling are fresh and tender, the nutrition is rich, the suaeda glauca seedling can be used as a vegetable, and also can be dried in the sun and ground into powder to be used for making wheaten food, cakes and the like, and the leaves contain about 50 percent of protein; the suaeda glauca seeds have the oil content of 26 percent, the content of unsaturated fatty acid in the oil accounts for 70 percent, and the suaeda glauca seeds have extremely high health care value and are particularly suitable for people suffering from hypertension, coronary heart disease and hyperlipidemia. Has the efficacies of preventing and treating various cancers, reducing biological accompanying fat and the like in the medical field, and has been paid attention at home and abroad. Salt land in the farming and animal husbandry of China is about 0.4 hundred million hm, common crops cannot be planted normally, and waste of land is caused. The suaeda salsa has excellent saline-alkali resistance and drought tolerance, and can normally grow in heavy saline-alkali soil. However, in the natural state, the germination rate of the suaeda salsa seeds is low, and no matter in wetland vegetation restoration or artificial cultivation, the invention of the method for promoting the germination of the suaeda salsa seeds is imperative. Based on the statement, the invention provides a method for promoting the germination of suaeda salsa seeds.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a method for promoting the germination of suaeda salsa seeds.
A method for promoting the germination of suaeda salsa seeds comprises the following steps:
s1, seed selection: selecting high-quality suaeda salsa seeds, soaking the seeds in water at the temperature of 20-30 ℃ for 2 hours, selecting the seeds with the bottom sinking, taking out and drying the seeds without peeling off the seeds before soaking;
s2, seed soaking treatment: preparing a potassium permanganate solution with the concentration of 1%, taking the seeds dried in the step S1, and placing the seeds in the prepared potassium permanganate solution for seed soaking treatment for 8 hours;
s3, germination culture: spreading the suaeda glauca seeds treated in the step S2 in a culture dish, putting the suaeda glauca seeds into an incubator for culturing until the suaeda glauca seeds germinate, wherein the culture temperature is 25 ℃, the illumination time is 12h/d, and the illumination intensity is 2000 lux;
and S4, calculating the germination rate according to the germination culture result in the step S3.
Preferably, the high-quality suaeda glauca seeds in the step S1 are suaeda glauca seeds matured and dropped in 11 months in the last year.
Preferably, the potassium permanganate solution in step S2 is analytically pure potassium permanganate.
Preferably, the relationship between the seed weight and the amount of the potassium permanganate solution in the step S2 is as follows: each 500g of seeds were soaked in 1L of potassium permanganate solution.
Preferably, the culture dish in the step S3 is padded with a double-layer filter paper, and 10ml of distilled water is added.
Preferably, the calculation formula of the seed germination rate in step S4 is as follows:
the method for promoting the germination of the suaeda salsa seeds, which is provided by the invention, has the advantages that the technical operation is simple, the problem of low germination rate of the suaeda salsa seeds is solved, the germination rate of the seeds is high, and the germination rate is improved to more than 88%.
Detailed Description
The present invention will be further illustrated with reference to the following specific examples.
Example one
The invention provides a method for promoting the germination of suaeda salsa seeds, which comprises the following steps:
s1, seed selection: selecting seeds of suaeda glauca which are mature and fall off in the last 11 months, soaking in water at 25 ℃ for 2h without peeling off the seed coats before soaking, selecting the seeds with the bottom sinking, taking out and drying in the air;
s2, seed soaking treatment: preparing 1% of analytically pure potassium permanganate solution, placing the seeds dried in the step S1 in the prepared potassium permanganate solution for soaking for 8 hours, wherein each 500g of the seeds are soaked in 1L of the potassium permanganate solution;
s3, germination culture: spreading the suaeda glauca seeds treated in the step S2 on a culture dish which is padded with two layers of filter paper, adding 10ml of distilled water into the culture dish, putting the culture dish into an incubator for culturing until the seeds germinate, wherein the culture temperature is 25 ℃, the illumination time is 12h/d, and the illumination intensity is 2000 lux;
s4, according to the germination culture result in the step S3 and according to the formula
And calculating the germination rate of the seeds.
Under the same culture conditions, the soaking in the potassium permanganate solution in the step S2 is replaced by soaking in distilled water, tap water, potassium nitrate and hydrogen peroxide, and a comparison test is carried out;
according to the culture scheme in the embodiment of the invention, the culture is repeated for three times, the results are averaged, and the germination time of the seeds is counted from sowing to germination (counting when the germs grow 1-2 mm), and is counted in hours (h). The germination rate of the seeds was calculated as follows:
comparative example No. two
The invention provides a method for promoting the germination of suaeda salsa seeds, which comprises the following steps:
the invention provides a method for promoting the germination of suaeda salsa seeds, which comprises the following steps:
s1, seed selection: selecting seeds of suaeda glauca which are mature and fall off in the last 11 months, soaking in water at 25 ℃ for 2h without peeling off the seed coats before soaking, selecting the seeds with the bottom sinking, taking out and drying in the air;
s2, seed soaking treatment: preparing 1% of analytically pure potassium permanganate solution, placing the seeds dried in the step S1 in the prepared potassium permanganate solution for soaking for 8 hours, wherein each 500g of the seeds are soaked in 1L of the potassium permanganate solution;
s3, germination culture: spreading the suaeda glauca seeds treated in the step S2 on a mat with two layers of filter paper, adding 10ml of 2% sodium chloride solution into a culture dish, placing the culture dish into an incubator for culturing until the seeds germinate, wherein the culture temperature is 25 ℃, the illumination time is 12h/d, and the illumination intensity is 2000 lux;
s4, according to the germination culture result in the step S3 and according to the formula
And calculating the germination rate of the seeds.
Under the same culture conditions, the soaking with the potassium permanganate solution in the step S2 was replaced by soaking without, distilled water, potassium nitrate and hydrogen peroxide, and comparative tests were performed.
According to the culture scheme in the embodiment of the invention, the culture is repeated for three times, the results are averaged, and the germination time of the seeds is counted from sowing to germination (counting when the germs grow 1-2 mm), and is counted in hours (h). The germination rate of the seeds was calculated as follows:
from table 1 in example one and table 2 in example two, it can be seen that: when the selected suaeda glauca seeds are soaked in 1% potassium permanganate solution for 8 hours and then placed into a culture dish to be cultured by distilled water, the highest germination rate is achieved, and the germination rate reaches 88.82%. Illustrating the feasibility of the present invention.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (4)
1. A method for promoting the germination of suaeda salsa seeds is characterized by comprising the following steps:
s1, seed selection: selecting high-quality suaeda salsa seeds, soaking the seeds in water at the temperature of 20-30 ℃ for 2 hours, selecting the seeds with the bottom sinking, taking out and drying the seeds without peeling off the seeds before soaking;
s2, seed soaking treatment: preparing a potassium permanganate solution with the concentration of 1%, taking the seeds dried in the step S1, and placing the seeds in the prepared potassium permanganate solution for seed soaking treatment for 8 hours;
s3, germination culture: spreading the suaeda glauca seeds treated in the step S2 in a culture dish, putting the suaeda glauca seeds into an incubator for culturing until the suaeda glauca seeds germinate, wherein the culture temperature is 25 ℃, the illumination time is 12h/d, and the illumination intensity is 2000 lux;
s4, calculating the germination rate according to the germination culture result in the step S3;
the high-quality suaeda glauca seeds in the step S1 are seeds of suaeda glauca which are ripe and fall off in 11 months in the last year, and the relationship between the weight of the seeds and the using amount of the potassium permanganate solution in the step S2 is as follows: each 500g of seeds were soaked in 1L of potassium permanganate solution.
2. The method for promoting the germination of the seeds of the suaeda salsa, according to claim 1, wherein the potassium permanganate solution in the step S2 is analytically pure potassium permanganate.
3. The method for promoting the germination of the seeds of suaeda salsa according to claim 1, wherein the double-layer filter paper is padded in the culture dish in the step S3, and 10ml of distilled water is added.
4. The method for promoting the germination of the suaeda salsa seeds as claimed in claim 1, wherein the calculation formula of the germination rate of the seeds in the step S4 is as follows: the seed germination rate is 100% of the number of germinated seeds/total number of seeds.
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| CN107509524A (en) * | 2017-09-27 | 2017-12-26 | 盐城工学院 | It is a kind of to promote the fluffy seed of alkali to sprout neat method and the fluffy implantation methods of alkali |
| CN109451914A (en) * | 2018-12-21 | 2019-03-12 | 中国科学院东北地理与农业生态研究所 | A method of improving the seed sprouting of seedling percent after the transplanting of saline-alkali wetland Suaeda heteroptera |
| CN115997518A (en) * | 2023-02-02 | 2023-04-25 | 新疆一路向前农业科技有限公司 | Device and method for repairing saline-alkali soil by utilizing suaeda salsa |
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| 盐碱胁迫对盐生植物种子萌发的影响;代莉慧 等;《干旱地区农业研究》;20121110;第30卷(第06期);第134页第2栏，第135页图1、第136页图3、第137页图5 * |
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Address after: 266100 Hongkong East Road, Laoshan District, Qingdao, Shandong Province, No. 7 Patentee after: QINGDAO University Address before: 266071 Shandong city of Qingdao province Ningxia City Road No. 308 Patentee before: QINGDAO University |