CN108991471A - A kind of natural additive for foodstuff with potent antioxidant activity - Google Patents
A kind of natural additive for foodstuff with potent antioxidant activity Download PDFInfo
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- CN108991471A CN108991471A CN201810678957.XA CN201810678957A CN108991471A CN 108991471 A CN108991471 A CN 108991471A CN 201810678957 A CN201810678957 A CN 201810678957A CN 108991471 A CN108991471 A CN 108991471A
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- anthocyanidin
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- 229910052742 iron Inorganic materials 0.000 description 1
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 230000003910 liver physiology Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000001630 malic acid Substances 0.000 description 1
- 235000011090 malic acid Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000000196 olfactory nerve Anatomy 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 201000007094 prostatitis Diseases 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000001525 retina Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000009759 skin aging Effects 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000012085 test solution Substances 0.000 description 1
- ANRHNWWPFJCPAZ-UHFFFAOYSA-M thionine Chemical compound [Cl-].C1=CC(N)=CC2=[S+]C3=CC(N)=CC=C3N=C21 ANRHNWWPFJCPAZ-UHFFFAOYSA-M 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical class OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000012224 working solution Substances 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/10—Natural spices, flavouring agents or condiments; Extracts thereof
- A23L27/105—Natural spices, flavouring agents or condiments; Extracts thereof obtained from liliaceae, e.g. onions, garlic
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
A kind of natural additive for foodstuff with potent antioxidant activity, belongs to technical field of food additives.By lycium ruthenicum anthocyanidin extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple onion anthocyanidin extraction purification object, granatanine extraction purification object, seaweed plants pigment extraction purification object, constituted according to weight ratio 30-40:30-40:10-20:2-10:2-10 mixing match.A kind of above-mentioned natural additive for foodstuff with potent antioxidant activity, it is its anti-oxidant, anti-radiation, anti-aging with super strength, antifatigue, nourishing liver and kidney, the effect for improving sleep, increasing immunity and Antimicrobial preservative, health nutrient value is high, performance is stablized, it is easy to store and transport for a long time, the deficiencies in the prior art are overcome, the added value of food is substantially increased.
Description
Technical field
The invention belongs to technical field of food additives, specially a kind of wholefood with potent antioxidant activity adds
Add agent.
Background technique
Food additives refer to improve the quality and color of food, while can extend the shelf-life of food and be added
Substance in food.With the rapid development of food industry, and in the current of " advocate green safe, return natural gray ",
Many food additives have the function of health nutrient.For example it is added with the vitamin C beneficial to human body, seaweeds, iron sheet
The food additives of dendrobium nobile, lucidum spore powder, microelement, amino acid etc. have the function such as good health care, anti-oxidant, anti-corrosion
Can, food additives refer to be effectively improved the quality of food and color, and to extend the storage life of food and
The chemical synthesis substance in food or natural materials are added.Food under certain environmental conditions, because the effect of microorganism can make
Food loses original nutritive value and putrid and deteriorated, and reason is mainly food apoilage caused by the breeding of microorganism.
Food preservative can prevent food because putrid and deteriorated caused by microbial reproduction, make food have in general natural environment compared with
Long storage life.
With the continuous improvement of people's dietetic level, food additives more and more come into daily life, and
Widely received by people.Health and health of the today's society consumer more concerned about oneself diet.Accordingly, with respect to food additives
Variety of problems, the also natural hot spot at people's common concern in terms of diet in recent years.The food kind that people eat is got over
Come more, it is which type of additive that whether focus more in food, which has additive, be added it health nutrient value where,
It is either with or without side effect etc..Therefore, it is pursuing color, smell, taste, shape, aesthetic quality and health value higher and higher today, is disappearing
Expense person will constantly pursue, and there is very highly nourishing health care effect, pure natural, edible safety, the wholefood having no toxic side effect to add
Add agent.
Anthocyanidin is one of most effective antioxidant of the plant origin found so far, and with super strength anti-oxidant and removing is certainly
By the activity of base, there is the superpower effect improved the immunity of the human body.Anthocyanidin is different from most of antioxidants, and free radical is to make
At the one of the major reasons of aging and many diseases, it is estimated that about 90% or so senile and degenerative disorders are all and free
Base is related, so anthocyanidin is anti-oxidant to human body, improves immunity extremely strong effect.Anthocyanidin belongs to natural sunlight covering
Object, can stop ultraviolet damage skin, and protecting human bodies skin is encroached on from free radical, moreover it is possible to repair injured collagen and bullet
Property fiber.Anthocyanidin can also improve the microcirculation of brain and heart, improve the fragility and permeability of capilary, slow down senile
And degenerative disorders.
Summary of the invention
For the above-mentioned problems in the prior art, it is an object of the invention to design to provide one kind with potent antioxygen
Change the technical solution of active natural additive for foodstuff, anti-oxidant, anti-radiation, anti-aging with super strength, antifatigue, tonifying liver
Kidney, the effect for improving sleep, increasing immunity and Antimicrobial preservative, health nutrient value is high, performance is stablized, be easy to store for a long time and
Transport, overcomes the deficiencies in the prior art, substantially increases the added value of food.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that by lycium ruthenicum anthocyanidin
Extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple onion anthocyanidin extraction purification object, granatanine extract
Purified, seaweed plants pigment extraction purification object, according to weight ratio 30-40:30-40:10-20:2-10:2-10 mixing match structure
At.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that the lycium ruthenicum flower
Green element extraction purification object is prepared using following methods: the black fruit fructus lycii of low-temperature air-drying being placed in historrhexis's extractor, is added
The ethanol solution room temperature that solid-to-liquid ratio is the 50%-70% of 3.0~pH of pH 4.0 of 1:2~1:5 is broken to extract 1-3 min.It filters,
The ethanol solution of the 50%-65% for 3.0~pH of pH 4.0 that solid-to-liquid ratio is 1:2~1:5 is added in gained filter residue, uses ultrasonic extraction instead
It is method room temperature ultrasonic extraction 1-2 times, 20-40 minutes each, it filters；Merge historrhexis's extracting solution and ultrasonic extraction liquid, in 50-
The 1/2-1/5 of original volume is concentrated under the conditions of 60 DEG C using flash concentration unit flashing, obtains concentrate；Concentrate is passed through big
Macroporous adsorbent resin Diaion HP 2MGL column chromatography carries out the enriching and purifying of total anthocyanidin；It is first eluted with distilled water, adjusts 0.5-
After the flow velocity of 1.5 mL/min is in colorless and transparent to eluent, uses 55~70% ethanol solution elution of pH 2.0-4.0 instead, receive
Collect the pigment eluent of aubergine, eluent flow rate is controlled in 0.5~1.5 mL/min；The aubergine eluent of collection is being revolved
Turn to be concentrated in vacuo dry or freeze-drying in evaporimeter, obtains black fruit fructus lycii anthocyanidin extraction purification object dry powder.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that the black pearl kind
Eggplant anthocyanidin extraction purification object is prepared using following methods: black pearl tomato being placed in historrhexis's extractor, volume is added
The ethanol solution room temperature of 55%-65% than 2.0~pH of pH 4.0 for 1:3~1:6 is broken to extract 1-3 min；It is squeezed with coarse cloth
Pressure obtains extracting solution；Extracting solution is concentrated under reduced pressure into 50-60 DEG C of water-bath using flash concentration device to the 1/3-1/ of original volume
6, obtain concentrate；Concentrate is carried out to the enrichment of total anthocyanidin by macroporous absorbent resin Diaion HP 2MGL column chromatography
Purifying；It is first eluted with distilled water, adjusts the flow velocity of 0.5-1.5 mL/min to eluent in after colorless and transparent, then with pH 3.0-
4.0 55~70% ethanol solution elution, collects the pigment eluent of aubergine, and eluent flow rate is controlled 0.5~1.5
mL/min；The aubergine eluent of collection is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, obtains black pearl tomato
Anthocyanidin extraction purification object dry powder.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that the purple onion
Anthocyanidin extraction purification object is prepared using following methods: purple onion being placed in historrhexis's extractor, addition volume ratio is 1:3
The ethanol solution room temperature of the 50%-60% of 3.0~pH of pH 4.0 of~1:6 is broken to extract 1-3 min；It filters, gained filter residue is added
Volume ratio is the ethanol solution of the 50%-70% of 3.0~pH of pH 4.0 of 1:3~1:6, is mentioned using ultrasonic extraction room temperature ultrasound
It takes 1 time, it is 20-40 minutes each, it filters；Merge historrhexis's extracting solution and ultrasonic extraction liquid, using sudden strain of a muscle under the conditions of 50-60 DEG C
Inspissation compression apparatus flash concentration obtains concentrate to the 1/2-1/6 of original volume；Concentrate is passed through into macroporous absorbent resin
Diaion HP 2MGL column chromatography carries out the enriching and purifying of purple Onion pigment；It is first eluted with distilled water, adjusts 0.5-1.5 mL/
The flow velocity of min is eluted to eluent in after colorless and transparent, then with 55~70% ethanol solution of pH 3.0-4.0, collection pigment
Eluent, eluent flow rate are controlled in 0.5~1.5 mL/min；By the pigment eluent of collection, vacuum is dense in Rotary Evaporators
Contracting is dry or is freeze-dried, and obtains black pearl tomato anthocyanidin extraction purification object dry powder.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that the granatum list
Peaceful extraction purification object is prepared using following methods: the granatum of low-temperature air-drying being placed in historrhexis's extractor, solid-liquid is added
The broken extraction 1-3 min of acetone soln room temperature than the 60%-70% for 1:4~1:8；It filters, by extracting solution in 50-60 DEG C of condition
The lower 1/4-1/8 that original volume is concentrated into using flash concentration unit flashing, obtained concentrate pass through 20 macropore of Diaion HP
Adsorbent resin column chromatography, respectively with liquid-to-solid ratio be the 1:20-50 times of deionized water measured, 10% ethyl alcohol, 20% ethyl alcohol, 40% ethyl alcohol,
60% ethyl alcohol and 80% ethyl alcohol carry out gradient elution according to the flow velocity of 2.5-10.0 mL/min, collect each eluent, merge 20% second
Alcohol, 40% ethyl alcohol, 60% ethanol eluate carry out flash concentration to the 1/10-1/20 of original volume in 50-60 DEG C of water-bath, obtain
Concentrate use Rotary Evaporators instead and carry out concentrated by rotary evaporation to dry, obtain granatanine extraction purification object dry powder.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that the seaweed plants
Pigment extraction purification object is prepared using following methods: dry seaweed being placed in historrhexis's extractor, solid-to-liquid ratio, which is added, is
The acetone room temperature of the 70%-80% of 1:3~1:8 is broken to extract 1-3 min；It filters, extracting solution is carried out to flash at 50-60 DEG C dense
It is reduced in concentrate without acetone；Then it is extracted 2-6 times with the ether that volume ratio is 1:1-1:3；Merge ether extraction liquid, 40
Normal pressure boils off ether in DEG C water-bath, and obtained concentrated extract is freeze-dried, and seaweed plants pigment extraction purification object is obtained.
A kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that lycium ruthenicum anthocyanidin mentions
Purified, black pearl tomato anthocyanidin extraction purification object, purple onion anthocyanidin extraction purification object, granatanine is taken to extract pure
Compound, the weight ratio of seaweed plants pigment extraction purification object are 32-38:32-38:12-18:3-8:3-8, preferably 34-35:34-
35:15-16:4-5:4-5。
Lycium ruthenicum, that is, black fruit fructus lycii belongs to Solanaceae Lycium.For more quil shrubs, multi-branched, branch is hard, the word for being often in
Shape bending, branch white.Black fruit fructus lycii is distributed mainly on the ground such as Qinghai, Ningxia, Xinjiang, Gansu, Shaanxi and the European Central Asia.
All have on Gansu, China Jiuquan, West of Qinghai Province, Ningxia, Xinjiang, Heilongjiang Province Huanan County east, West Inner Mongolia and Tibet and other places
Fragmentary distribution.It is grown in barren hill, the riverbed sandy beach that the mankind can not survive, and possesses extremely strong vitality.Lycium ruthenicum tool
There are various bioactivity, specifically include that 1, anti-aging, improve sleep: lot of anthocyanin contained in lycium ruthenicum has very
Good anti-oxidant, elimination human free radical, has the function of significant anti-aging.Anthocyanidin can pass through blood-brain barrier, can protect
Cranial nerve is not oxidized, can stablize cerebral tissue function, protects injury of the brain not by harmful chemical and toxin.Eat black Chinese holly
After anthocyanidin in Qi, sleep quality can be significantly improved.2, anti-radiation, improvement skin: the anthocyanidin in lycium ruthenicum is day
Right sunlight overcover can prevent injury of the ultraviolet light to skin, can prevent generating ahead of time for wrinkle of skin, more can Replacement Battalion
Support and eliminate harmful free radical in vivo.3, nourishing liver and kidney, anti-asthenopia: the anthocyanidin in lycium ruthenicum can promote retina cell
The regeneration of middle visual purple can prevent laser high myopia and detached retina, have effects that anti-asthenopia and promote eyesight.4, change
Kind circulation, strengthen immunity: the anthocyanidin in lycium ruthenicum can improve blood circulation, restore the capilary effect lost, reinforce
Blood vessel elasticity provides the immunity of body.In addition, the anthocyanidin in lycium ruthenicum also has hypoglycemic, decompression, adjusts lipid metaboli,
The effect of protecting Cardiovascular System.
Black pearl tomato originates in South America, is the treasure of tomato family, gains the name due to its fruit color and fruit are black.Its
Fruit is dietotherapeutic, has strong fruit aroma, and the mouthfeel of moderately sour and sweet is easily absorbed by the body, and nutritive value is high, especially
It is suitble to fresh food.Black pearl tomato is the first-filial generation elite seed introduced from foreign countries, has mouthfeel good, colours excellent, pol height,
Harvest the advantages that uniformity is good, and disease resistance is strong.Containing high 10 times of the Lycopene than in Fructus Lycopersici esculenti in black pearl tomato, also containing big
The antioxidants such as the anthocyanidin of amount and vitamin C.Anthocyanidin in black pearl tomato can generate strong biology in human body
Effect, have shield kidney and enhancing body, treatment prostatitis, prevention breast cancer, cervix cancer, prostate cancer, colon cancer with it is cardiopathic
Effect, edible black tomato can reduce male and suffer from prostate cancer and cardiopathic probability.
Onion belongs to 2 years raw herbaceous plant of Liliaceae allium.Onion is a kind of collection vegetables, drug, condiment, sterilization and health care
Multi-functional, the multiduty food being integrated.Purple onion appearance aubergine, purple onion are rich in anthocyanidin, and anthocyanidin facilitates
Color is provided for water fruits and vegetables, it belongs to the flavonoids in fruits and vegetables, and the body of people oneself cannot generate anthocyanidin, mainly by taking the photograph
Dark food materials are taken to supplement.Purple onion kill human cancer cell is most effective, this is because the kind contains more antioxidant
Anthocyanidin and Quercetin.Purple onion can activate the path for encouraging cancer cell death, they promote the formation of cancer cell adverse environment,
The communication between cancer cell is destroyed, to inhibit the growth of tumour.Studies have shown that due to a large amount of cyanine of content in purple onion
Element, Quercetin and other biological activities substance, therefore effectively prevent skin aging, brain tonic eyeshield, protection liver kidney, remove body
Interior rubbish, purification blood prevent three height and cardiovascular and cerebrovascular disease, eliminate carcinogen, prevention arthritis, liver kidney portion illness, resist
Cancer and bactericidal effect.
The fragrant and sweet deliciousness of pomegranate is summer most popular one of fruit, full of nutrition, and whole body is all precious up and down.Pomegranate
Skin is the pericarp of Punicaceae plant pomegranate.Contain the ingredients such as malic acid, tannin in granatum.Tannin is also known as tannin, is to be present in
The intracorporal complicated polyhydric phenols of one kind of plant.Studies have shown that granatum has apparent antibacterial and convergence function,
Intestinal mucosa can be made to restrain, so that intestines is sticked cured secretion and reduce, so diarrhea can be effectively treated；Granatum contains matter of mixing more, in addition to
Have the effects that also there is hemostasis and astriction outside anti-oxidant antifatigue, removing free radical well.
Algal pigment mainly contains carotenoid, including astaxanthin, it and lycopene etc. are all carotenogenesis
Intermediate product.It is widely present in living nature, such as shrimp, crab, fish, frond, the leaf of plant, flower are main in marine organisms body in fruit
One of carotenoid.Astaxanthin pigment in algae is a kind of carotenoid based colorant, is strongest in the world
One of natural can effectively clear intracellular oxygen radical, enhance cytothesis ability, has anti-aging, alleviates
Sports fatigue is invigorated, the occurrence and development of the chronic diseases such as pre- preventing tumor, cardiovascular disease, diabetes, delay senescence etc.
The effect of aspect.Astaxanthin can be across barrier from blood to brain, so astaxanthin is to brain, central nervous system and double
Eye can all play a protective role, and can alleviate asthenopia symptom, improve visual sensitivity；With ultraviolet radiation preventing, alleviate pigment
It is calm；Cell factor is adjusted, inflammatory cytokine is inhibited to show；The anti-inflammatory series of characteristics such as anti-infective.Algae is nature
Astaxanthin-containing biology abundant in boundary, is known as the concentrate of natural astaxanthin.Using algae from ocean and fresh water algae
Extracting astaxanthin has vast potential for future development, it has also become the research hotspot of natural astaxanthin production in the world.
A kind of above-mentioned natural additive for foodstuff with potent antioxidant activity, has the advantages that
1. being added according to the pigmentary structures such as anthocyan, tannins, carotenoid heat, the unstability of wet, light, air
The factors such as heat, humidity, air, light influence and are easier under solution state the rotten feature that is oxidized and degrades, black extracting
When fruit fructus lycii anthocyanidin and purple Onion pigment, the present invention combines room temperature texture's Smashing extraction method and room temperature ultrasonic extraction,
By room temperature texture it is broken extract effect ingredient in plant tissue cell in a short time rapidly and efficiently turn be dissolved in solvent
In, in conjunction with room temperature ultrasonic extraction, mentions use up functional component in a relatively short period of time；Extracting black pearl tomato anthocyanidin, stone
It, will broken and extraction process in a few minutes clock time using room temperature texture's Smashing extraction method when pomegranate skin tannin and seaweed plants pigment
It is combined into one, has been completed at the same time the process for being crushed, mix, stir, homogenizing and extracting, has made in histocyte in a short time
Ingredient is quickly transferred in solvent, is realized and is extracted quick and complete high efficiency extraction, and guarantees functional component to greatest extent
Structure is not destroyed, it is ensured that the high storage rate and the rate of transform of functional component.
2. existing synthesis antioxidant, although the advantage low, inexpensive with production cost, its toxic side effect
Hidden danger is brought to the application of these food additives.In comparison, natural additive for foodstuff has environmentally protective, nontoxic secondary work
With, use safe advantage.Existing natural inhibitor is mostly tea polyphenols, vitamin E, flavonoids, Polyphenols.As food
The drawback that product additive has at high cost, anti-oxidant and Scavenging ability not strong enough.Food additives of the invention, raw material
It is green safe from pure natural, it is without any side effects.It is anti-oxidant, anti-aging with super strength, antifatigue and remove free radical
Activity, it is anti-oxidant and remove free radical activity be far longer than the strong antioxidants such as tea polyphenols, vitamin E, antioxidant activity
It is tea polyphenols and the decades of times of VE.
3. being present in the anthocyanidin in various fruits and vegetables and plant, tannins and carotenoid pigment is with strong anti-oxidation
Active ingredient, it is not easy to maintain after these fruit-vegetable plants are mature, it is easy to rot, and ingress of air is oxidized quickly.And it is new
Fresh fruits and vegetables largely use, because containing more carbohydrate, general population should not be largely used for a long time, it is necessary to add in time
Work is made into fruit wine, jam etc. such as disinfection, canning sealing after squeezing the juice, but all has sugar content more, and unsuitable most of crowds use
Etc. drawbacks.The present invention is to carry out low temperature rapidly extracting and flash concentration by special technique and technology, then passes through macropore tree
Extraction purification of the effect of rouge enriching and purifying eliminates a large amount of sugar, protein and other impurities, obtains high-content except ingredient
Object overcomes directly edible fruit-vegetable plant bring adverse effect.
4. five kinds of natural plants are from different fruit-vegetable plants, and contained oxidation-resistant active ingredient type is different, including flower
The effect of green element class, tannins, carotenoids, three kinds of different types of structure ingredient efficient combination, played mutually coordinated
Synergistic effect greatly strengthens its anti-oxidant and removing free radical activity.Show this with clinical application by largely testing
Natural additive for foodstuff anti-oxidant, anti-aging with super strength, it is antifatigue and remove free radical activity, also with health care
Effect.
5. the natural additive for foodstuff anti-oxidant, anti-radiation, anti-aging with super strength, antifatigue, nourishing liver and kidney, improvement are slept
It sleeps, the effect of increase immunity and Antimicrobial preservative, health nutrient value is abundant, performance is stablized, it is easy to storage for a long time and transport, gram
The deficiencies in the prior art are taken.Due to having carried out the packaging of two steps, air and moisture is completely cut off, have avoided the shadow of illumination
It rings, increases the stability of functional component and shelf-life in product.As food additives, varieties of food items and cake can be added to
In point, beautiful, anti-oxidant and obvious health-care efficacy bright in colour.
Specific embodiment
Below in conjunction with specific embodiment, the invention will be further described.Based on the embodiments of the present invention, this field is general
Logical technical staff every other embodiment obtained without making creative work belongs to what the present invention protected
Range.
Embodiment 1: using lycium ruthenicum anthocyanidin extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple ocean
Green onion anthocyanidin extraction purification object, granatanine extraction purification object, seaweed plants pigment extraction purification object, according to weight ratio 40:
30:15:5:10 mixing match is sealed according to every 10g and is protected from light pack, is put into brown sealed plastic bottle to obtain the final product.
The black fruit fructus lycii anthocyanidin extraction purification object the preparation method is as follows: the black fruit fructus lycii of low-temperature air-drying is placed in
In historrhexis's extractor, 1.5 min of the broken extraction of 60% ethanol solution room temperature for the pH 3.0 that solid-to-liquid ratio is 1:4 are added.It takes out
Filter, gained filter residue are added 60% ethanol solution for the pH 3.0 that solid-to-liquid ratio is 1:4, use ultrasonic extraction room temperature ultrasonic extraction 1 instead
It is secondary, it 30 minutes every time, filters.Merge historrhexis's extracting solution and ultrasonic extraction liquid, is filled under the conditions of 55 DEG C using flash concentration
Flash concentration is set to the 1/5 of original volume, obtains concentrate.Concentrate is passed through into macroporous absorbent resin Diaion HP 2MGL column
Chromatography carries out the enriching and purifying of total anthocyanidin.It is first eluted with distilled water, it is in colourless for adjusting flow velocity to the eluent of 1.5 mL/min
After transparent, the 65% ethanol solution elution of pH 4.0 being used instead, collecting the pigment eluent of aubergine, eluent flow rate control exists
1.5 mL/min.The aubergine eluent of collection is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, obtains black fruit Chinese holly
Qi anthocyanidin extraction purification object dry powder, weighing calculate yield, and sample is protected from light kept dry.Obtained black fruit fructus lycii anthocyanidin extracts
The yield of purified is 14.5%, and the content of total anthocyanidin reaches 46.6% in extraction purification object.
The black pearl tomato extraction purification object extracts the preparation method is as follows: black pearl tomato is placed in historrhexis
In device, the broken extraction 1min of 55% ethanol solution room temperature for the pH 3.0 that volume ratio is 1:4 is added.It is squeezed, is mentioned with coarse cloth
Take liquid.Extracting solution is concentrated under reduced pressure into the 1/5 of original volume using flash concentration device in 60 DEG C of water-baths, obtains concentrate.It will
Concentrate carries out the enriching and purifying of total anthocyanidin by macroporous absorbent resin Diaion HP 2MGL column chromatography.First use distilled water
Elution adjusts the flow velocity of 1.0 mL/min to eluent in after colorless and transparent, then with the 55% ethanol solution elution of pH 3.0,
The pigment eluent of aubergine is collected, eluent flow rate is controlled in 1.5 mL/min.The aubergine eluent of collection is being rotated
It is concentrated in vacuo dry or freeze-drying in evaporimeter, obtains black pearl tomato anthocyanidin extraction purification object dry powder, weighing calculates yield,
Sample is protected from light kept dry.The yield of obtained black pearl tomato anthocyanidin extraction purification object is 6.5%, total in extraction purification object
The content of anthocyanidin reaches 25.1.
The purple Onion pigment extraction purification object the preparation method is as follows: purple onion is placed in historrhexis's extractor
In, 1 min of the broken extraction of 60% ethanol solution room temperature for the pH 4.0 that volume ratio is 1:4 is added.It filters, gained filter residue is added
Volume ratio is 60% ethanol solution of the pH pH 4.0 of 1:5, using ultrasonic extraction room temperature ultrasonic extraction 1 time, extracts 20 points
Clock filters.Merge historrhexis's extracting solution and ultrasonic extraction liquid, is concentrated under the conditions of 50 DEG C using flash concentration unit flashing
The 1/4 of original volume, obtains concentrate.Concentrate is subjected to purple ocean by macroporous absorbent resin Diaion HP 2MGL column chromatography
The enriching and purifying of green onion pigment.It is first eluted with distilled water, after the flow velocity of 1.5 mL/min of adjusting is in colorless and transparent to eluent, then is used
65% ethanol solution of pH 4.0 elutes, and collects pigment eluent, and eluent flow rate is controlled in 1.5 mL/min.By collection
Pigment eluent is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, and it is dry to obtain black pearl tomato anthocyanidin extraction purification object
Powder, weighing calculate yield, and sample is protected from light kept dry.The yield of obtained purple Onion pigment extraction purification object is 10.5%, is extracted
The content of total pigment reaches 18.4% in purified.
The granatanine extraction purification object the preparation method is as follows: the granatum of low-temperature air-drying is placed in tissue
In smashing extractor, 3 min of the broken extraction of 70% acetone soln room temperature that solid-to-liquid ratio is 1:8 are added.It filters, extracting solution is existed
It is concentrated into the 1/8 of original volume using flash concentration unit flashing under the conditions of 60 DEG C, obtained concentrate passes through Diaion HP 20
Large pore resin absorption column chromatography, respectively with liquid-to-solid ratio be the 1:30 times of deionized water measured, 10% ethyl alcohol, 20% ethyl alcohol, 40% ethyl alcohol,
60% ethyl alcohol and 80% ethyl alcohol carry out gradient elution according to the flow velocity of 8.0 mL/min, collect each eluent, merge 20% ethyl alcohol,
40% ethyl alcohol, 60% ethanol eluate, carry out flash concentration to the 1/20 of original volume in 50-60 DEG C of water-bath, obtained concentrate
It uses Rotary Evaporators instead and carries out concentrated by rotary evaporation to doing, obtain granatanine extraction purification object dry powder.Weighing calculates yield, sample
It is protected from light kept dry.The yield of obtained granatanine extraction purification object is 16.3%.
The seaweed plants pigment extraction purification object mentions the preparation method is as follows: dry seaweed is placed in historrhexis
It takes in device, the broken extraction 1-3 min of 70% acetone room temperature that solid-to-liquid ratio is 1:8 is added.It filters, by extracting solution at 50-60 DEG C
It carries out flash concentration and is free of acetone into concentrate.Then it is extracted 4 times with the ether that volume ratio is 1:2.Merge ether extraction liquid,
Normal pressure boils off ether in 40 DEG C of water-baths, and obtained concentrated extract is freeze-dried, and obtains seaweed plants pigment extraction purification
Object.Weighing calculates yield, and sample is protected from light kept dry.The yield of obtained seaweed plants pigment extraction purification object is 9.4%.
Embodiment 2: using lycium ruthenicum anthocyanidin extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple ocean
Green onion anthocyanidin extraction purification object, granatanine extraction purification object, seaweed plants pigment extraction purification object, according to weight ratio 35:
40:10:5:10 mixing match is sealed according to every 10g and is protected from light pack, is put into brown sealed plastic bottle to obtain the final product.
The black fruit fructus lycii anthocyanidin extraction purification object the preparation method is as follows: the black fruit fructus lycii of low-temperature air-drying is placed in
In historrhexis's extractor, 2 min of the broken extraction of 50% ethanol solution room temperature for the pH 3.0 that solid-to-liquid ratio is 1:5 are added.It takes out
Filter, gained filter residue are added 50% ethanol solution for the pH 3.00 that solid-to-liquid ratio is 1:5, use ultrasonic extraction room temperature ultrasonic extraction instead
It 1 time, 20 minutes every time, filters.Merge historrhexis's extracting solution and ultrasonic extraction liquid, is filled under the conditions of 60 DEG C using flash concentration
Flash concentration is set to the 1/5 of original volume, obtains concentrate.Concentrate is passed through into macroporous absorbent resin Diaion HP 2MGL column
Chromatography carries out the enriching and purifying of total anthocyanidin.It is first eluted with distilled water, it is in colourless for adjusting flow velocity to the eluent of 1.5 mL/min
After transparent, the 60% ethanol solution elution of pH 4.0 being used instead, collecting the pigment eluent of aubergine, eluent flow rate control exists
1.0 mL/min.The aubergine eluent of collection is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, obtains black fruit Chinese holly
Qi anthocyanidin extraction purification object dry powder, weighing calculate yield, and sample is protected from light kept dry.Obtained black fruit fructus lycii anthocyanidin extracts
The yield of purified is 14.4%, and the content of total anthocyanidin reaches 51.2% in extraction purification object.
The black pearl tomato extraction purification object extracts the preparation method is as follows: black pearl tomato is placed in historrhexis
In device, 2 min of the broken extraction of 55% ethanol solution room temperature for the pH 4.0 that volume ratio is 1:6 are added.It is squeezed, is obtained with coarse cloth
Extracting solution.Extracting solution is concentrated under reduced pressure into the 1/6 of original volume using flash concentration device in 50-60 DEG C of water-bath, is concentrated
Liquid.Concentrate is carried out to the enriching and purifying of total anthocyanidin by macroporous absorbent resin Diaion HP 2MGL column chromatography.First with steaming
Distilled water elution adjusts the flow velocity of .5 mL/min and washes to eluent in after colorless and transparent, then with 65% ethanol solution of pH4.0
It is de-, the pigment eluent of aubergine is collected, eluent flow rate is controlled in 1.0 mL/min.The aubergine eluent of collection is being revolved
Turn to be concentrated in vacuo dry or freeze-drying in evaporimeter, obtain black pearl tomato anthocyanidin extraction purification object dry powder, weighing calculates
Rate, sample are protected from light kept dry.The yield of obtained black pearl tomato anthocyanidin extraction purification object is 8.2%, in extraction purification object
The content of total anthocyanidin reaches 29.7%.
The purple Onion pigment extraction purification object the preparation method is as follows: purple onion is placed in historrhexis's extractor
In, 1 min of the broken extraction of 60% ethanol solution room temperature for the pH 3.0 that volume ratio is 1:5 is added.It filters, gained filter residue is added
Volume ratio is 60% ethanol solution of the pH 3.0 of 1:4, using ultrasonic extraction room temperature ultrasonic extraction 1 time, every time 25 minutes,
It filters.Merge historrhexis's extracting solution and ultrasonic extraction liquid, is concentrated under the conditions of 50-60 DEG C using flash concentration unit flashing
The 1/5 of original volume, obtains concentrate.Concentrate is subjected to purple ocean by macroporous absorbent resin Diaion HP 2MGL column chromatography
The enriching and purifying of green onion pigment.It is first eluted with distilled water, after the flow velocity of 1.5 mL/min of adjusting is in colorless and transparent to eluent, then is used
70% ethanol solution of pH 4.0 elutes, and collects pigment eluent, and eluent flow rate is controlled in 1.5 mL/min.By collection
Pigment eluent is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, and it is dry to obtain black pearl tomato anthocyanidin extraction purification object
Powder, weighing calculate yield, and sample is protected from light kept dry.The yield of obtained purple Onion pigment extraction purification object is 11.2%, is extracted
The content of total pigment reaches 19.6% in purified.
The granatanine extraction purification object the preparation method is as follows: the granatum of low-temperature air-drying is placed in tissue
In smashing extractor, 3 min of the broken extraction of 70% acetone soln room temperature that solid-to-liquid ratio is 1:6 are added.It filters, extracting solution is existed
It is concentrated into the 1/6 of original volume using flash concentration unit flashing under the conditions of 60 DEG C, obtained concentrate passes through Diaion HP 20
Large pore resin absorption column chromatography, respectively with liquid-to-solid ratio be the 1:25 times of deionized water measured, 10% ethyl alcohol, 20% ethyl alcohol, 40% ethyl alcohol,
60% ethyl alcohol and 80% ethyl alcohol carry out gradient elution according to the flow velocity of 7.0 mL/min, collect each eluent, merge 20% ethyl alcohol,
40% ethyl alcohol, 60% ethanol eluate carry out flash concentration to the 1/20 of original volume in 60 DEG C of water-baths, and obtained concentrate is used instead
Rotary Evaporators carry out concentrated by rotary evaporation to doing, and obtain granatanine extraction purification object dry powder.Weighing calculates yield, and sample is protected from light
Kept dry.The yield of obtained granatanine extraction purification object is 16.1%.
The seaweed plants pigment extraction purification object mentions the preparation method is as follows: dry seaweed is placed in historrhexis
It takes in device, 3 min of the broken extraction of 75% acetone room temperature that solid-to-liquid ratio is 1:6 is added.Filter, by extracting solution at 50-60 DEG C into
Row flash concentration is free of acetone into concentrate.Then it is extracted 3 times with the ether that volume ratio is 1:2.Merge ether extraction liquid,
Normal pressure boils off ether in 40 DEG C of water-baths, and obtained concentrated extract is freeze-dried, and obtains seaweed plants pigment extraction purification object.
Weighing calculates yield, and sample is protected from light kept dry.The yield of obtained seaweed plants pigment extraction purification object is 11.8%.
Embodiment 3: using lycium ruthenicum anthocyanidin extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple ocean
Green onion anthocyanidin extraction purification object, granatanine extraction purification object, seaweed plants pigment extraction purification object, according to weight ratio 40:
37:10:5:8 mixing match is sealed according to every 10g and is protected from light pack, is put into brown sealed plastic bottle to obtain the final product.
The black fruit fructus lycii anthocyanidin extraction purification object the preparation method is as follows: the black fruit fructus lycii of low-temperature air-drying is placed in
In historrhexis's extractor, 1.5 min of the broken extraction of 55% ethanol solution room temperature for the pH 3.0 that solid-to-liquid ratio is 1:5 are added.It takes out
Filter, gained filter residue are added 55% ethanol solution for the pH 3.0 that solid-to-liquid ratio is 1:2, use ultrasonic extraction room temperature ultrasonic extraction 2 instead
It is secondary, it 20 minutes every time, filters.Merge historrhexis's extracting solution and ultrasonic extraction liquid, is filled under the conditions of 55 DEG C using flash concentration
Flash concentration is set to the 1/4 of original volume, obtains concentrate.Concentrate is passed through into macroporous absorbent resin Diaion HP 2MGL column
Chromatography carries out the enriching and purifying of total anthocyanidin.It is first eluted with distilled water, it is in colourless for adjusting flow velocity to the eluent of 1.5 mL/min
After transparent, the 65% ethanol solution elution of pH4.0 being used instead, collecting the pigment eluent of aubergine, eluent flow rate control exists
1.5 mL/min.The aubergine eluent of collection is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, obtains black fruit Chinese holly
Qi anthocyanidin extraction purification object dry powder, weighing calculate yield, and sample is protected from light kept dry.Obtained black fruit fructus lycii anthocyanidin extracts
The yield of purified is 14.6%, and the content of total anthocyanidin reaches 55.2% in extraction purification object.
The black pearl tomato extraction purification object extracts the preparation method is as follows: black pearl tomato is placed in historrhexis
In device, the broken extraction 2min of 60% ethanol solution room temperature for the pH pH 4.0 that volume ratio is 1:5 is added.It is squeezed, is obtained with coarse cloth
Obtain extracting solution.Extracting solution is concentrated under reduced pressure into the 1/5 of original volume using flash concentration device in 60 DEG C of water-baths, is concentrated
Liquid.Concentrate is carried out to the enriching and purifying of total anthocyanidin by macroporous absorbent resin Diaion HP 2MGL column chromatography.First with steaming
Distilled water elution adjusts the flow velocity of 1.0 mL/min and washes to eluent in after colorless and transparent, then with 60% ethanol solution of pH 4.0
It is de-, the pigment eluent of aubergine is collected, eluent flow rate is controlled in 1.5 mL/min.The aubergine eluent of collection is being revolved
Turn to be concentrated in vacuo dry or freeze-drying in evaporimeter, obtain black pearl tomato anthocyanidin extraction purification object dry powder, weighing calculates
Rate, sample are protected from light kept dry.The yield of obtained black pearl tomato anthocyanidin extraction purification object is 6.9%, in extraction purification object
The content of total anthocyanidin reaches 31.3%.
The purple Onion pigment extraction purification object the preparation method is as follows: purple onion is placed in historrhexis's extractor
In, the broken extraction 1min of 50% ethanol solution room temperature for the pH 4.0 that volume ratio is 1:4 is added.It filters, body is added in gained filter residue
60% ethanol solution of the product than the pH 4.0 for being 1:5 is extracted 30 minutes using ultrasonic extraction room temperature ultrasonic extraction 1 time, is taken out
Filter.Merge historrhexis's extracting solution and ultrasonic extraction liquid, substance is concentrated into using flash concentration unit flashing under the conditions of 55 DEG C
Long-pending 1/5, obtains concentrate.Concentrate is subjected to purple onion color by macroporous absorbent resin Diaion HP 2MGL column chromatography
The enriching and purifying of element.It is first eluted with distilled water, after the flow velocity of 1.5 mL/min of adjusting is in colorless and transparent to eluent, then is used
60% ethanol solution of pH4.0 elutes, and collects pigment eluent, and eluent flow rate is controlled in 1.5 mL/min.By the color of collection
Plain eluent is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, and it is dry to obtain black pearl tomato anthocyanidin extraction purification object
Powder, weighing calculate yield, and sample is protected from light kept dry.The yield of obtained purple Onion pigment extraction purification object is 9.8%, is extracted
The content of total pigment reaches 20.3% in purified.
The granatanine extraction purification object the preparation method is as follows: the granatum of low-temperature air-drying is placed in tissue
In smashing extractor, the broken extraction 1-3 min of 66% acetone soln room temperature that solid-to-liquid ratio is 1:7 is added.It filters, by extracting solution
It is concentrated into the 1/7 of original volume using flash concentration unit flashing under the conditions of 60 DEG C, obtained concentrate passes through Diaion HP
20 large pore resin absorption column chromatographies are respectively the 1:30 times of deionized water measured, 10% ethyl alcohol, 20% ethyl alcohol, 40% second with liquid-to-solid ratio
Alcohol, 60% ethyl alcohol and 80% ethyl alcohol carry out gradient elution according to the flow velocity of 10.0 mL/min, collect each eluent, merge 20% second
Alcohol, 40% ethyl alcohol, 60% ethanol eluate, carry out flash concentration to the 1/20 of original volume in 60 DEG C of water-baths, obtained concentrate
It uses Rotary Evaporators instead and carries out concentrated by rotary evaporation to doing, obtain granatanine extraction purification object dry powder.Weighing calculates yield, sample
It is protected from light kept dry.The yield of obtained granatanine extraction purification object is 16.7%.
The seaweed plants pigment extraction purification object mentions the preparation method is as follows: dry seaweed is placed in historrhexis
It takes in device, 2.5 min of the broken extraction of 75% acetone room temperature that solid-to-liquid ratio is 1:7 is added.Filter, by extracting solution at 55 DEG C into
Row flash concentration is free of acetone into concentrate.Then it is extracted 4 times with the ether that volume ratio is 1:2.Merge ether extraction liquid,
Normal pressure boils off ether in 40 DEG C of water-baths, and obtained concentrated extract is freeze-dried, and obtains seaweed plants pigment extraction purification object.
Weighing calculates yield, and sample is protected from light kept dry.The yield of obtained seaweed plants pigment extraction purification object is 17.4%.
Beneficial effects of the present invention are further proved below by way of the test data of test.
1, embodiment 1-3 is anti-oxidant and anti-fatigue test
1) the measurement test of anti-oxidant and free-radical scavenging activity
The measurement of DPPH free radical scavenging ability: DPPH method is one of the common method of free radical scavenging ability detection, sample measurement
Make positive control with Trolox in the process, and the total oxidation resistance of sample is conversed with this.Measurement result is expressed as reaching
To Trolox concentration required for the comparable oxidation resistance of a certain concentration test substances.This method is referred to as TEAC method.TEAC value
Sample is equivalent to the content of Trolox when being expressed as reaching half elimination ratio.TEAC value is bigger, shows to remove free radical energy
Power is stronger.IC50Value is the parameter for being frequently used for evaluation oxidation resistance, it refers to the DPPH of antioxidant for clearing 50% certainly
Required concentration when by base.Its value is smaller, and when expression reaches 50% clearance rate, the concentration dose of free radical scavenger used is got over
Small, radicals scavenging effect is also better, and corresponding sample antioxidant activity of participating in the experiment is stronger.Measuring method: one is accurately weighed
Quantitative DPPH reagent, is dissolved, and be quantitatively transferred in 100 mL measuring bottles with 95% ethyl alcohol, with 95 % ethyl alcohol constant volumes, shakes up centainly dense
The DPPH stock solution of degree, be placed in refrigerator refrigerate it is spare.In 96 hole elisa Plates respectively plus various concentration sample solution and
DPPH test solution after concussion after 24 DEG C of 30 min of heat preservation, measures its light absorption value (Ap), while measurement is not added under 517 nm wavelength
The sample blank light absorption value (Ac) of DPPH and adds DPPH but the light absorption value (Amax) of sample is not added.With the concentration of each test sample
It is that ordinate draws directrix curve with the free radical scavenging activity (Y) measured, according to regression equation calculation IC for abscissa50.Free radical
Clearance rate=1- (Ap-Ac)/Amax × 100% converses the TEAC value of sample, evaluates oxidation resistance with this.
The measurement of FRAB oxidation resistance: 10mmolL is taken respectively-1TPTZ(be dissolved in 40mmolL-1Hydrochloric acid),
20mmol·L-1Liquor ferri trichloridi and 0.lmolL-1Acetate buffer solution (pH3.6), mixed with the volume ratio of 1:1:10
Uniformly obtain FRAP reagent.Precision measures sample solution and FRAP reagent, its extinction is surveyed in the two reaction at 593nm after ten minutes
Angle value.It takes equivalent dehydrated alcohol to substitute testing sample solution, while appropriate FRAP working solution is added as blank control.Most
Afterwards, the antioxidant activity power of sample can be calculated according to the size of absorbance value.Absorbance value size and anti-oxidant energy
Power power is proportional, i.e., absorbance value is bigger, and antioxidant activity is stronger, and in other words, FRAP value is bigger, and antioxidant activity is got over
By force.
The total reducing power measurement of TRP: the Trolox comparison liquid of sample solution and 5 concentration gradients is taken, phosphoric acid is separately added into
In buffer, 1% K is then added3Fe(CN)6, after heating 30 minutes, 10 % trichloroacetic acids are added after being placed at room temperature for, mix
It is centrifuged 10 min afterwards.2.5 mL of supernatant is taken, distilled water is rapidly added and 0.1% ferric trichloride is mixed, at 700 nm of wavelength
Carry out absorbance measurement.Using Trolox concentration as abscissa, standard curve is drawn by ordinate of absorbance, method is same as above.Knot
Fruit indicates with Trolox equivalent TEAC, evaluates oxidation resistance with this.It is shown in Table 1.
The antioxidant activity test result (TEAC value, unit: mg TE/g DW) of 1 embodiment 1-3 of table
| Sample | DPPH method | TRP method | FRAP method |
| Embodiment 1 | 798.4 | 123.3 | 68.3 |
| Embodiment 2 | 823.6 | 134.5 | 72.6 |
| Embodiment 3 | 812.9 | 129.8 | 72.1 |
Test result shows embodiment 1-3 oxidation resistance with super strength, and activity that is anti-oxidant and removing free radical is much
Greater than antioxidant such as tea polyphenols, vitamin Es, antioxidant activity is equivalent to tea polyphenols and the decades of times of VE.
2) anti-fatigue test of mice burden swimming
The cleaning grade male mice in kunming for taking weight 18-22g, with dexamethasone sodium phosphate injection 0.5mg/ only intramuscular note
It penetrates, continuous injection 7 days prepares kidney-yang deficiency animal model.Mouse is randomly divided into 6 groups, respectively Normal group, model group,
Positive group, high dose group (3.0g/kg) of the embodiment 1 as drug, middle dose group (1.5g/kg) and low dose group (0.75g/
Kg), every group 10, each group give test medicine by 0.1mL/10g is oral respectively, and Normal group and model group are during administration
The dosage physiological saline such as injection, the red drink heavily material of dosage such as injection when positive group is administered.One time a day, successive administration 15 days.It is every group small
Mouse is randomly divided into two groups, and one group is used for Loaned swimming test, and another group for taking mouse hepatic glycogen, serum urea and serum lactic
Carry out assay.
The antifatigue experiment of mice burden swimming: first by animal training 3 times, all mouse are in last dose 30min postposition
Mandatory swimming test is done in swimming trunk.The sheet lead of 10% weight of mouse tail root load.Record is from swim to mouse head
The time that portion's whole submerged cannot emerge as the final exhausted swimming time (s), is shown in Table 2.With normal control
Group is compared, and the mice burden swimming time of kidney-yang deficiency model group significantly reduces.And compared with model group, with the increasing of dosage
Add, the mice burden swimming time gradually extends.Wherein walking weight load of the high dose group of embodiment 1-3 to mice with kidney-yang deficiency
It is extended more, illustrate that embodiment 1-3 has the function of good antifatigue and tonifying kidney and strengthening yang.
The measurement of hepatic glycogen content: the storage capacity of hepatic glycogen can directly affect the locomitivity of body.Therefore glycogen contains
Amount can illustrate the speed or degree of fatigue generation.After embodiment 1-3 is as the last dose 30min of drug, mouse is placed in water
Not swimming with a load attached to the body 90min in the swimming trunk of 25 DEG C of temperature takes out mouse cervical dislocation and puts to death taking-up liver after swimming.By liver physiology
After saline rinse, the content of glycogen kit and microplate reader measurement hepatic glycogen is used.Measurement result is shown in Table 3.It can from table 3
Out, for model group compared with normally group mouse, hepatic glycogen content has conspicuousness reduction.And the hepatic glycogen content of each medicine group mouse is bright
It is aobvious to increase.With the increase of drug dose, hepatic glycogen content also obviously increases therewith.Or even medicine group high dose group hepatic glycogen
Content is higher than positive group, and middle dose group content is suitable with positive group.Show that the various dose group of embodiment 1-3 is shown very
Strong liver glycogen reserves function, therefore embodiment 1-3 has stronger anti-oxidant and antifatigue effect.
The assay of blood lactase acid: the most common biochemistry of body degree of fatigue refers to when blood lactase acid measurement is evaluation strenuous exercise
Mark.After 30 min of mouse last dose, then venous blood collection stops in 30 DEG C of water after not swimming with a load attached to the body 10min, respectively at small
0 min, 30 min acquire blood to mouse before swimming, after swimming, and centrifuging and taking serum measures blood using lactic acid testing cassete and microplate reader
Clear lactic acid content.It being compared with Normal group, kidney-yang deficiency model group mouse serum lactic content after swimming, which has, to be obviously increased,
And blood lactate concentration decline is very slow after 30 min of rest.And medicine group mouse is after swimming, and serum cream after 30 min that rested
Acid concentration declines clearly, especially high and middle dose group can be most significant reduce the serum lactic after deficiency of yang mouse movement and contain
Amount shows that embodiment 1-3 has and significantly relieves fatigue effect.It is shown in Table 4.
2, embodiment 1-3 is antifatigue to age groups crowd, Oxidation Resistance Test effect
To causing the young crowd of asthenopia using embodiment 1-3 with computer cell phone for a long time, compare the effectiveness results relieved fatigue
It is shown in Table 5.
To the work of long-term excessive overload, pressure is excessive, energy is not concentrated, has not enough sleep, body & mind over fatigue, face
50-60 years old dark and gloomy crowd uses embodiment 1-3, observes improvement after a period of use, the results are shown in Table 6.
Embodiment 1-3 to have an old and weathered face, weak and sickly, few gas is powerless, memory is decreased obviously, face has the 60-70 of senile plaque
Year, old man carried out experimental observation, after embodiment 1-3 tri- months, in terms of playing anti-oxidant, anti-aging and improving immunity
Improvement.It the results are shown in Table 7.
。
Claims (8)
1. a kind of natural additive for foodstuff with potent antioxidant activity, it is characterised in that by lycium ruthenicum anthocyanidin extraction purification
Object, black pearl tomato anthocyanidin extraction purification object, purple onion anthocyanidin extraction purification object, granatanine extraction purification object,
Seaweed plants pigment extraction purification object is constituted according to weight ratio 30-40:30-40:10-20:2-10:2-10 mixing match.
2. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that described
Lycium ruthenicum anthocyanidin extraction purification object using following methods prepare: by the black fruit fructus lycii of low-temperature air-drying be placed in historrhexis extract
In device, the broken extraction 1-3 of ethanol solution room temperature of the 50%-70% for 3.0~pH of pH 4.0 that solid-to-liquid ratio is 1:2~1:5 is added
min。
3. filtering, the ethanol solution of the 50%-65% for 3.0~pH of pH 4.0 that solid-to-liquid ratio is 1:2~1:5 is added in gained filter residue, is changed
It is 20-40 minutes each with ultrasonic extraction room temperature ultrasonic extraction 1-2 times, it filters；Merge historrhexis's extracting solution and ultrasound mentions
Liquid is taken, the 1/2-1/5 of original volume is concentrated into using flash concentration unit flashing under the conditions of 50-60 DEG C, obtains concentrate；It will be dense
Contracting liquid carries out the enriching and purifying of total anthocyanidin by macroporous absorbent resin Diaion HP 2MGL column chromatography；First drenched with distilled water
It washes, after the flow velocity of adjusting 0.5-1.5 mL/min is in colorless and transparent to eluent, uses 55~70% ethyl alcohol of pH 2.0-4.0 instead
Solution elution, collects the pigment eluent of aubergine, and eluent flow rate is controlled in 0.5~1.5 mL/min；By the purplish red of collection
Color eluent is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, obtains black fruit fructus lycii anthocyanidin extraction purification object dry powder.
4. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that described
Black pearl tomato anthocyanidin extraction purification object using following methods prepare: black pearl tomato is placed in historrhexis's extractor
In, the broken extraction 1-3 of ethanol solution room temperature of the 55%-65% for 2.0~pH of pH 4.0 that volume ratio is 1:3~1:6 is added
min；It is squeezed with coarse cloth, obtains extracting solution；Extracting solution is concentrated under reduced pressure into original using flash concentration device in 50-60 DEG C of water-bath
The 1/3-1/6 of volume, obtains concentrate；Concentrate is carried out always by macroporous absorbent resin Diaion HP 2MGL column chromatography
The enriching and purifying of anthocyanidin；First eluted with distilled water, after the flow velocity of adjusting 0.5-1.5 mL/min is in colorless and transparent to eluent,
It is eluted again with 55~70% ethanol solution of pH 3.0-4.0, collects the pigment eluent of aubergine, eluent flow rate control exists
0.5~1.5 mL/min；The aubergine eluent of collection is concentrated in vacuo dry or freeze-drying in Rotary Evaporators, is obtained black
Peral tomato anthocyanidin extraction purification object dry powder.
5. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that described
Purple onion anthocyanidin extraction purification object using following methods prepare: purple onion is placed in historrhexis's extractor, be added
The ethanol solution room temperature that volume ratio is the 50%-60% of 3.0~pH of pH 4.0 of 1:3~1:6 is broken to extract 1-3 min；It filters,
The ethanol solution of the 50%-70% for 3.0~pH of pH 4.0 that volume ratio is 1:3~1:6 is added in gained filter residue, using ultrasonic extraction
Method room temperature ultrasonic extraction 1 time, it is 20-40 minutes each, it filters；Merge historrhexis's extracting solution and ultrasonic extraction liquid, at 50-60 DEG C
Under the conditions of the 1/2-1/6 of original volume is concentrated into using flash concentration unit flashing, obtain concentrate；Concentrate is inhaled by macropore
Attached resin Diaion HP 2MGL column chromatography carries out the enriching and purifying of purple Onion pigment；It is first eluted with distilled water, adjusts 0.5-
The flow velocity of 1.5 mL/min is eluted to eluent in after colorless and transparent, then with 55~70% ethanol solution of pH 3.0-4.0, receipts
Collect pigment eluent, eluent flow rate is controlled in 0.5~1.5 mL/min；By the pigment eluent of collection in Rotary Evaporators
Vacuum concentration is dry or is freeze-dried, and obtains black pearl tomato anthocyanidin extraction purification object dry powder.
6. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that described
Granatanine extraction purification object using following methods prepare: the granatum of low-temperature air-drying is placed in historrhexis's extractor
In, the broken extraction 1-3 min of acetone soln room temperature for the 60%-70% that solid-to-liquid ratio is 1:4~1:8 is added；It filters, extracting solution is existed
The 1/4-1/8 of original volume is concentrated under the conditions of 50-60 DEG C using flash concentration unit flashing, obtained concentrate passes through Diaion
20 large pore resin absorption column chromatography of HP, respectively with liquid-to-solid ratio be the 1:20-50 times of deionized water measured, 10% ethyl alcohol, 20% ethyl alcohol,
40% ethyl alcohol, 60% ethyl alcohol and 80% ethyl alcohol carry out gradient elution according to the flow velocity of 2.5-10.0 mL/min, collect each eluent,
Merge 20% ethyl alcohol, 40% ethyl alcohol, 60% ethanol eluate, the 1/10- of flash concentration to original volume is carried out in 50-60 DEG C of water-bath
1/20, obtained concentrate uses Rotary Evaporators instead and carries out concentrated by rotary evaporation to doing, and obtains granatanine extraction purification object dry powder.
7. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that described
Seaweed plants pigment extraction purification object using following methods prepare: dry seaweed is placed in historrhexis's extractor, is added
Enter the broken extraction 1-3 min of acetone room temperature for the 70%-80% that solid-to-liquid ratio is 1:3~1:8；It filters, by extracting solution at 50-60 DEG C
It carries out flash concentration and is free of acetone into concentrate；Then it is extracted 2-6 times with the ether that volume ratio is 1:1-1:3；Merge ether
Extract liquor, normal pressure boils off ether in 40 DEG C of water-baths, and obtained concentrated extract is freeze-dried, and obtains seaweed plants pigment and mentions
Take purified.
8. a kind of natural additive for foodstuff with potent antioxidant activity as described in claim 1, it is characterised in that black Chinese holly
Qi anthocyanidin extraction purification object, black pearl tomato anthocyanidin extraction purification object, purple onion anthocyanidin extraction purification object, granatum
Tannin extraction purification object, seaweed plants pigment extraction purification object weight ratio be 32-38:32-38:12-18:3-8:3-8, preferably
34-35:34-35:15-16:4-5:4-5。
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