CN108991002A - A kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin and its application - Google Patents

A kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin and its application Download PDF

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Publication number
CN108991002A
CN108991002A CN201810969406.9A CN201810969406A CN108991002A CN 108991002 A CN108991002 A CN 108991002A CN 201810969406 A CN201810969406 A CN 201810969406A CN 108991002 A CN108991002 A CN 108991002A
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ningnanmycin
benzene metsulfovax
bactericidal composition
agent
composition containing
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詹敏敏
冯亚东
杨的妹
柴明根
甄长征
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JIANGXI HEYI CHEMICALS Co Ltd
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JIANGXI HEYI CHEMICALS Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N47/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
    • A01N47/08Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
    • A01N47/10Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
    • A01N47/12Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/48Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
    • A01N43/541,3-Diazines; Hydrogenated 1,3-diazines

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin, its effective component is benzene metsulfovax and Ningnanmycin, the mass ratio of benzene metsulfovax and Ningnanmycin is 50:1-1:50, remaining for allow in pesticide using with acceptable auxiliary element.Bactericidal composition of the present invention can be prepared into suspending agent, wettable powder, water dispersible granules, microemulsion, aqueous emulsion, granule and suspension seed-coating agent by known method.The present composition has apparent synergistic effect, can be used for preventing and treating the plurality of plant diseases on the crops such as vegetables, melon and fruit, is particularly suitable for prevention and treatment downy mildew, late blight and powdery mildew.

Description

A kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin and its application
Technical field
The present invention relates to a kind of bactericidal composition, especially a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin And its application of disease on crops is prevented and treated, belong to technical field of pesticide.
Background technique
Benzene metsulfovax, English name: Benthiavalicarb-isopropyl, chemical name: [[(6- fluoro benzothiazole- 2- yl)-ethylaminocarbonyl] -2- methyl-propyl] carbamic acid isopropyl ester.Benzene metsulfovax are by Japanese combinatorial chemistry company The new type bactericide developed jointly with Ihara company in 1992, active site is substitution fluorine atom and amino acid on phenyl ring Peptide structure, the compound have it is very highly selective, the late blight and downy mildew mainly caused to crop by Oomycete germ has Good prevention effect and therapeutic activity.
Ningnanmycin, English name: Ningnanmycin, molecular formula: C16H23O8N7,.Ningnanmycin is by Chinese science The innovation biological pesticide that institute's Chengdu biological study is succeeded in developing is Cystosine nucleocide type antibiotics, it is by extending disease The incubation period of bacterium destroys virion structure, reduces virion concentration and adjusting organism metabolism is disease-resistant to enhance plant Ability and immunity.At present mainly for crop virosis such as prevention and treatment tomato, capsicum, tobaccos.
In recent years, due to single drug and unscientific application method, lead to many kinds of pathogens such as powdery mildew, downy mildew Bacterium etc. produces resistance to many currently used pesticides, and drug resistance is increasingly severe, while formulation rate after resistance generation With the increase of spraying times, not only increase control cost, and aggravated environmental pollution, become chemical prevention one is big Problem.Therefore, it is badly in need of microbicide compositions efficiently, environmentally friendly, less toxic.The mechanism of action of benzene metsulfovax and Ningnanmycin fungicide Difference mutually compounds and expands fungicidal spectrum in range on one point, improves the bactericidal effect of fungicide, avoid or delay disease drug resistance Generation, be conducive to the integrated control of crop disease.In conclusion Bactericidal composite of the exploitation containing benzene metsulfovax and Ningnanmycin Object is of great significance.
Summary of the invention
It is suitble to agriculturally produce and use the object of the present invention is to provide a kind of, high-efficiency environment friendly, wide spectrum and helps to subtract Few dosage delays pathogen to generate resistance and reduces the bactericidal composition of use cost.
A kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin, effective component are that benzene metsulfovax and Ningnan are mould The mass ratio of element, benzene metsulfovax and Ningnanmycin is 50:1-1:50.
Preferably, the mass ratio of effective component benzene metsulfovax and Ningnanmycin is 20:1-1:20.
Preferably, the gross mass percentage composition of effective component benzene metsulfovax and Ningnanmycin in bactericidal composition is 1%- 90%。
The present invention also provides a kind of applications of bactericidal composition containing benzene metsulfovax and Ningnanmycin, by selecting not Same adjuvant, the dosage form of the bactericidal composition can be suspending agent, wettable powder, water dispersible granules, microemulsion, water and milk Agent, granule and suspension seed-coating agent.
Preferably, the bactericidal composition is used to prevent and treat the plurality of plant diseases on the crops such as vegetables, melon and fruit, especially suitable In prevention and treatment downy mildew, late blight and powdery mildew.
Compared with prior art, the present invention the beneficial effects are as follows:
1, bactericidal composition of the invention has good synergistic effect in range on one point, and control efficiency obviously mentions compared with single dose It is high;
2, field dosage can be greatly decreased, reduce production and application cost, reduce pesticide residue, be conducive to environment Sustainable development;
3, the mechanism of action of effective component is different in bactericidal composition, is conducive to the generation for avoiding or delaying disease drug resistance;
4, expand prevention spectrum, while preventing and treating crop downy mildew, late blight and powdery mildew, virosis can be prevented and treated.
Specific embodiment
In order to keep the objectives, technical solutions, and advantages of the present invention more clear, the following specific implementation of the present invention Example is described in detail, but the invention is not limited to these examples, and that is done all within the spirits and principles of the present invention any repairs Change, equivalent replacement and improvement etc., should all be included in the protection scope of the present invention.Therefore, the scope of protection of the patent of the present invention It should be subject to the appended claims.
Different pesticide active ingredients is compounded, is drug effect and resistance etc. in current solution pesticide single dose application process A kind of effective means of problem.After different pesticide active ingredients is compounded, three kinds of type of action are typically exhibited, that is, is added and makees It with, synergistic effect and antagonism, but is specially which kind of effect, it is unpredictable, only just it can know that by a large number of experiments.Compounding Synergy is formulated well, can be improved practical control efficiency, be reduced the usage amount of pesticide, facilitate the generation of delaying drug resistance, be section Learn the important means of controlling disease.
Bactericidal composition of the present invention, for a kind of effective component, is another effective component with Ningnanmycin with benzene metsulfovax, Combination between them has obvious synergistic function downy mildew, late blight and powdery mildew, rather than just two kinds of medicines The simple addition of effect effect, is specifically illustrated with following bioassay example.
Assays Example 1, benzene metsulfovax compound the indoor toxicity determination test to cucumber downy mildew with Ningnanmycin
Subjects: cucumber downy mildew germ
This test uses pot-culture method.It is dipped to pick up from artificial climate room under 10 DEG C or so of distillation washing with writing brush and has downy mildew The sporangium at the cucumber leaves back side of germ, is made into 2.0-3 × 105The suspension of a sporangium/mL.Choose growing way consistent two Piece leaf period cucumber seedling, each processing select 5 basins for trying melon seedling, spraying under 50PSI pressure with Potter spray tower, each place Manage about 5mL.5 concentration gradients are arranged in each medicament, to spray equivalent clear water as blank control.Chemicals treatment is sprayed afterwards for 24 hours Mist is inoculated with bacterium of downy mildew of cucumber sporangia suspension, and cucumber seedling is placed in growth cabinet (relative humidity 100%, temperature after inoculation 15-20 DEG C of degree) culture, it keeps 16-24 DEG C of temperature, 90% or so moisturizing of relative humidity to induce afterwards for 24 hours, the state of an illness is investigated after 5 days and is referred to Number, and calculate control efficiency.
Grade scale
0 grade: blade disease-free spot;
1 grade: lesion area accounts for 5% or less full wafer leaf area;
3 grades: lesion area accounts for the 6%-10% of full wafer leaf area;
5 grades: lesion area accounts for the 11%-25% of full wafer leaf area;
7 grades: lesion area accounts for the 26%-50% of full wafer leaf area;
9 grades: lesion area accounts for 50% or more full wafer leaf area.
Drug effect calculation method:
Disease index (%)=
Control efficiency (%)=
Control efficiency is converted into probability value (y), and liquor strength (μ g/mL) is converted into logarithm (x), calculates poison with least square method Concentration EC in power equation and inhibition50, the toxicity index and co-toxicity coefficient (CTC) of medicament are calculated according to Sun Yunpei's Method.
Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50)×100
B in percentage composition+B medicament toxicity index × mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index × mixture Percentage composition
Co-toxicity coefficient (CTI)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)] × 100
When CTC≤80, then composition shows as antagonism, and when 80 < CTC < 120, then composition shows as summation action, when CTC >=120, then composition shows as synergistic effect.
The Toxicity Determination result is referring to table 1
1 benzene metsulfovax of table compound the The Toxicity Determination result to cucumber downy mildew with Ningnanmycin
Test result shows that benzene metsulfovax compound prevention and treatment cucumber downy mildew with Ningnanmycin, when matching between 50:1-1:50, Co-toxicity coefficient is all 120.0 or more, and when having certain synergistic effect, and matching between 10:1-1:10, co-toxicity coefficient is high In 200.0, synergistic effect is become apparent from.
Assays Example 2, benzene metsulfovax compound the indoor toxicity determination test to tomato late blight with Ningnanmycin
Subjects: tomato late blight germ
This test uses pot-culture method.When tomato potting grows to 2-4 leaf periods, number spare.Test is existed with pathogen It is cultivated on suitable culture medium, after sporangium to be generated, sporangium is washed down with sterile water, is filtered with double gauze, it is made 1 × 105The suspension of a sporangium/mL.According to agent activity, 5-7 series mass concentration is set.Medical fluid is uniformly sprayed on leaf Face is spare to medical fluid natural air drying to whole wettings.3 basins of every processing, 4 repetitions, and set containing only solvent and surfactant and Blank control is made in processing without effective component.Chemicals treatment for 24 hours after, with crops sprayer on tomato leaf uniformly sprinkling connect Kind.After inoculation, cultivated 7 days under conditions of 18-20 DEG C of temperature, 90% or more relative humidity.Count the disease on every plant of tomato leaf Spot area, investigation prevention effect.30 leaves, stage division are at least investigated in every processing are as follows:
0 grade: blade disease-free spot;
1 grade: only a small amount of small scab, lesion area account for 10% or less full wafer leaf area on blade;
3 grades: lesion area accounts for the 10%-25% of full wafer leaf area on blade;
5 grades: lesion area accounts for the 26%-50% of full wafer leaf area on blade;
7 grades: lesion area accounts for 50% or more of full wafer leaf area on blade;
9 grades: full leaf morbidity is withered.
Drug effect calculates, toxicity index and co-toxicity coefficient (CTC) calculating, analysis method are same as above.
The Toxicity Determination result is referring to table 2
2 benzene metsulfovax of table compound the The Toxicity Determination result to tomato late blight with Ningnanmycin
Test result shows that benzene metsulfovax compound prevention and treatment tomato late blight with Ningnanmycin, when matching between 50:1-1:50, Co-toxicity coefficient is all 120.0 or more, and when having certain synergistic effect, and matching between 10:1-1:10, co-toxicity coefficient is high In 200.0, synergistic effect is become apparent from.
Assays Example 3, benzene metsulfovax compound the indoor toxicity test examination to cucumber powdery mildew's pathogen with Ningnanmycin It tests
Subjects: cucumber powdery mildew's pathogen
This test uses pot-culture method.Potting grows to 4-5 piece true leaf, numbers spare.By interior generation for 24 hours on cucumber leaves of falling ill Powdery Mildew Fresh spores are uniformly shaken off to be inoculated on examination cucumber seedling.According to agent activity, it is dense that 5-7 series mass is set Degree.After inoculation 24 hours, medical fluid is uniformly sprayed on to blade face to whole wettings, it is spare to medical fluid natural air drying.3 basins of every processing, 4 Secondary repetition, and set containing only solvent and surfactant and make blank control without containing the processing for imitating ingredient.Test material after inoculation is moved Into growth cabinet, (relative humidity 100%, 20 ± 2 DEG C of temperature) is cultivated 7 days.The lesion area on every basin cucumber leaves is counted, Disease index is investigated, and calculates control efficiency.
Grade scale
0 grade: blade disease-free spot;
1 grade: lesion area accounts for 5% or less full wafer leaf area;
3 grades: lesion area accounts for the 6%-15% of full wafer leaf area;
5 grades: lesion area accounts for the 16%-25% of full wafer leaf area;
7 grades: lesion area accounts for the 26%-50% of full wafer leaf area;
9 grades: lesion area accounts for 50% or more full wafer leaf area.
Drug effect calculates, toxicity index and co-toxicity coefficient (CTC) calculating, analysis method are same as above.
The Toxicity Determination result is referring to table 3
3 benzene metsulfovax of table compound the The Toxicity Determination result to cucumber powdery mildew's pathogen with Ningnanmycin
Test result shows that benzene metsulfovax compound prevention and treatment cucumber powdery mildew's pathogen with Ningnanmycin, matches between 50:1-1:50 When, co-toxicity coefficient is all in 120.0 or more, when having certain synergistic effect, and matching between 10:1-1:10, co-toxicity coefficient 200.0 are above, synergistic effect becomes apparent from.
The above measurement result shows that benzene metsulfovax compound prevention and treatment cucumber downy mildew, tomato late blight and Huang with Ningnanmycin When the proportion of melon powdery mildew is between 50:1-1:50, co-toxicity coefficient all 120.0 or more, has certain synergistic effect, and matches When than between 10:1-1:10,200.0 or more, synergistic effect becomes apparent from co-toxicity coefficient.
Combination sterilization composition of the present invention can be prepared into suspending agent, the wettable of suitable agricultural use by known method Pulvis, water dispersible granules, microemulsion, aqueous emulsion, granule and suspension seed-coating agent.It is illustrated, is matched with specific embodiment below Percentage is mass percent in side, and benzene metsulfovax raw medicine effective content is 95%, Ningnanmycin mother's medicine effective content is 40%, Effective content folding hundred is pressed when use to calculate, and is not being repeated below.
Suspending agent embodiment
By each components such as pesticide active ingredient, dispersing agent, wetting agent, thickener, defoaming agent, antifreezing agent and water in the ratio of formula It is uniformly mixed, obtains half product after sand mill wet process sand milling, water supplement, which is uniformly mixed, after analysis filters up to suspending agent.
Embodiment 1:31% benzene metsulfovax Ningnanmycin suspending agent
Benzene metsulfovax 30%, Ningnanmycin 1%, ethylene glycol (antifreezing agent) 5%, pull open powder (wetting agent) 2%, polycarboxylate (dispersion Agent) 2%, xanthan gum (thickener) 0.15%, aluminium-magnesium silicate (thickener) 0.5%, sodium benzoate (preservative) 0.2%, organosilicon disappear Infusion (defoaming agent) 0.1%, deionized water polishing to 100%.
Embodiment 2:10.5% benzene metsulfovax Ningnanmycin suspending agent
Benzene metsulfovax 0.5%, Ningnanmycin 10%, ethylene glycol (antifreezing agent) 5%, pull open powder (wetting agent) 1.5%, lignin sulfonic acid Sodium (dispersing agent) 3%, aluminium-magnesium silicate (thickener) 0.5%, sodium benzoate (preservative) 0.2%, has xanthan gum (thickener) 0.2% Machine silicon defoaming agent (defoaming agent) 0.1%, deionized water polishing to 100%.
Wettable powder embodiment
Pesticide active ingredient, auxiliary agent and filler are mixed according to the formula uniformly, remix to obtain after air-flow crushing wettable Property pulvis.
Embodiment 3:51% benzene metsulfovax Ningnanmycin wettable powder
Benzene metsulfovax 50%, Ningnanmycin 1%, lauryl sodium sulfate (wetting agent) 2%, Negel (dispersing agent) 3%, Sodium lignin sulfonate (dispersing agent) 7%, white carbon black (filler) 5%, kaolin (filler) polishing to 100%.
Embodiment 4:32% benzene metsulfovax Ningnanmycin wettable powder
Benzene metsulfovax 2%, Ningnanmycin 30%, lauryl sodium sulfate (wetting agent) 2%, Negel (dispersing agent) 2%, Sodium lignin sulfonate (dispersing agent) 4%, white carbon black (filler) 5%, kaolin (filler) polishing to 100%.
Embodiment 5:90% benzene metsulfovax Ningnanmycin wettable powder
Benzene metsulfovax 88%, Ningnanmycin 2%, lauryl sodium sulfate (wetting agent) 1%, polycarboxylate (dispersing agent) 1%, kaolinite Native (filler) polishing is to 100%.
Water dispersible granules embodiment
Pesticide active ingredient, auxiliary agent and filler are mixed according to the formula uniformly, wettable powder is obtained after air-flow crushing, then A certain amount of water is added, through kneading, granulation, drying, sieves obtained water dispersible granules.
Embodiment 6:10% benzene metsulfovax Ningnanmycin water dispersible granules
Benzene metsulfovax 5%, Ningnanmycin 5%, lauryl sodium sulfate (wetting agent) 2%, Negel (dispersing agent) 2%, Sodium lignin sulfonate (dispersing agent) 4%, polycarboxylate (dispersing agent) 2%, ammonium sulfate (disintegrating agent) 15%, kaolin (filler) polishing To 100%.
Embodiment 7:52% benzene metsulfovax Ningnanmycin water-dispersible grain
Benzene metsulfovax 50%, Ningnanmycin 2%, lauryl sodium sulfate (wetting agent) 4%, sodium lignin sulfonate (dispersing agent) 7%, Negel (dispersing agent) 2%, polycarboxylate (dispersing agent) 2%, ammonium sulfate (disintegrating agent) 10%, kaolin (filler) polishing To 100%.
Embodiment 8:22% benzene metsulfovax Ningnanmycin water dispersible granules
Benzene metsulfovax 20%, Ningnanmycin 2%, lauryl sodium sulfate (wetting agent) 2%, Negel (dispersing agent) 6%, sodium lignin sulfonate (dispersing agent) 4%, ammonium sulfate (disintegrating agent) 15%, kaolin (filler) polishing to 100%.
Microemulsion embodiment
Pesticide active ingredient is dissolved in solvent and cosolvent, surfactant is added, is uniformly mixed and obtains microemulsion.
Embodiment 9:20.5% benzene metsulfovax Ningnanmycin microemulsion
Benzene metsulfovax 0.5%, Ningnanmycin 20%, N-Methyl pyrrolidone (solvent) 4%, phenethyl phenol polyethenoxy ether (emulsification Agent) 6%, calcium dodecyl benzene sulfonate (emulsifier) 4%, castor oil polyoxyethylene ether (emulsifier) 6%, deionized water polishing extremely 100%。
Aqueous emulsion embodiment
Pesticide active ingredient is dissolved in solvent and cosolvent, surfactant is added, is uniformly mixed and obtains oily phase;It will be water-soluble The property components such as component and deionized water are mixed to prepare water phase, then during the oil phase is added to the aqueous phase, stirring while adding, high speed shear is allowed to Aqueous emulsion is obtained as uniform emulsion.
Embodiment 10:15% benzene metsulfovax Ningnanmycin aqueous emulsion
Benzene metsulfovax 5%, Ningnanmycin 10%, ethylene glycol (antifreezing agent) 5%, N-Methyl pyrrolidone (solvent) 4%, cyclohexanone (solvent) 9%, phenethyl phenol polyethenoxy ether (emulsifier) 7%, calcium dodecyl benzene sulfonate (emulsifier) 5%, deionized water polishing To 100%.
Granule embodiment
Embodiment 11:5.5% benzene metsulfovax Ningnanmycin granule
Benzene metsulfovax 0.5%, Ningnanmycin 5%, fatty alcohol polyoxyethylene ether (emulsifier) 1%, N-Methyl pyrrolidone (solvent) 5%, white carbon black (adsorbent) 3%, brick slag (carrier) polishing to 100%.
Embodiment 12:1% benzene metsulfovax Ningnanmycin granule
Benzene metsulfovax 0.5%, Ningnanmycin 0.5%, fatty alcohol polyoxyethylene ether (emulsifier) 1%, N-Methyl pyrrolidone are (molten Agent) 1%, white carbon black (adsorbent) 2%, brick slag (carrier) polishing to 100%.
Suspension seed-coating agent embodiment
By each components such as pesticide active ingredient, dispersing agent, wetting agent, thickener, defoaming agent, antifreezing agent and water in the ratio of formula It is uniformly mixed, obtains half product after sand mill wet process sand milling, water supplement, film forming agent and colorant are uniformly mixed filtering after analysis Up to suspension seed-coating agent.
Embodiment 13:10.5% benzene metsulfovax Ningnanmycin suspension seed-coating agent
Benzene metsulfovax 0.5%, Ningnanmycin 10%, ethylene glycol (antifreezing agent) 5%, sodium methylene bis-naphthalene sulfonate (wetting agent) 1%, wood Quality sodium sulfonate (dispersing agent) 3%, polycarboxylate (dispersing agent) 1%, xanthan gum (thickener) 0.15%, aluminium-magnesium silicate (thickener) 1%, polyvinyl alcohol (PVA) (film forming agent) 0.5%, solvent red (HRR) (colorant) 2%, sodium benzoate (preservative) 0.2%, organic Silicon defoaming agent (defoaming agent) 0.1%, deionized water polishing to 100%.
Field information example 1: benzene metsulfovax compound the field control effectiveness test to powdery mildew of cucumber with Ningnanmycin
Subjects: powdery mildew of cucumber
Test medicine and dosage are shown in Table 4, and every 4 cells of processing repeat, 20 square metres of each plot area.In the early stages of disease Carry out first time application, even spraying is sprayed onto until blade drips, using clear water processing as blank control.It is applied after 7 days second Medicine.The incidence of 7 days investigation powderys mildew of cucumber before first time medicine and after second of medicine.Each cell is sampled using 5 points, Every is investigated two plants, and every plant of all blades of investigation account for the percentage of entire leaf area with lesion area to be classified and record.It calculates each Handle disease index and control efficiency.Grade scale:
0 grade: disease-free spot;
1 grade: lesion area accounts for 5% or less entire leaf area;
3 grades: lesion area accounts for the 6%-15% of entire leaf area;
5 grades: lesion area accounts for the 16%-25% of entire leaf area;
7 grades: lesion area accounts for the 26%-50% of entire leaf area;
9 grades: lesion area accounts for entire 50% or more leaf area;
Drug effect calculation method:
Disease index=
Control efficiency (%)=
Field test results are shown in Table 4
4 benzene metsulfovax of table compound the field test results to powdery mildew of cucumber with Ningnanmycin
As can be seen that benzene metsulfovax, Ningnanmycin single dose are respectively to the preventive effect of powdery mildew of cucumber from 4 test result of table 74.98%, 70.80%, and bactericidal composition of the embodiment of the present invention is significantly increased in preventive effect, minimum preventive effect is 84.03%, It has been up to 92.34%.The result of field trial sufficiently shows after benzene metsulfovax are compounded with Ningnanmycin to powdery mildew of cucumber With significant synergistic function, in the case where mu effective ingredient consumption is reduced compared with single dose, control efficiency is greatly improved.Cause This, the present composition have reduce cost, delaying drug resistance, reduce spraying times and pesticide residue beneficial effect.

Claims (5)

1. a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin, it is characterised in that: its effective component is benzene metsulfovax With Ningnanmycin, the mass ratio of benzene metsulfovax and Ningnanmycin is 50:1-1:50.
2. a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin according to claim 1, it is characterised in that: have The mass ratio for imitating ingredient benzene metsulfovax and Ningnanmycin is 20:1-1:20.
3. a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin according to claim 1, it is characterised in that: have Imitating the gross mass percentage composition of ingredient benzene metsulfovax and Ningnanmycin in bactericidal composition is 1%-90%.
4. a kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin according to claim 3, it is characterised in that: logical It crosses and selects different adjuvants, the dosage form of the bactericidal composition can be suspending agent, wettable powder, water dispersible granules, micro- Emulsion, aqueous emulsion, granule and suspension seed-coating agent.
5. a kind of application of the bactericidal composition according to claim 1 containing benzene metsulfovax and Ningnanmycin, feature Be: the bactericidal composition is used to prevent and treat the plurality of plant diseases on the crops such as vegetables, melon and fruit, is particularly suitable for prevention and treatment downy mildew Disease, late blight and powdery mildew.
CN201810969406.9A 2018-08-24 2018-08-24 A kind of bactericidal composition containing benzene metsulfovax and Ningnanmycin and its application Pending CN108991002A (en)

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刘彦良等: "宁南霉素和多抗霉素对黄瓜白粉病的控制作用研究", 《农药科学与管理》 *

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Application publication date: 20181214