CN108935086A - A kind of chemical seed-soaking liquid and preparation method thereof for corn doubling monoploids - Google Patents
A kind of chemical seed-soaking liquid and preparation method thereof for corn doubling monoploids Download PDFInfo
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- CN108935086A CN108935086A CN201810920405.5A CN201810920405A CN108935086A CN 108935086 A CN108935086 A CN 108935086A CN 201810920405 A CN201810920405 A CN 201810920405A CN 108935086 A CN108935086 A CN 108935086A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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Abstract
The invention discloses a kind of chemical seed-soaking liquids for corn doubling monoploids, including raw material below: sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl sulphoxide aqueous solution, trefanocide aqueous solution.The invention also discloses the preparation methods of the chemical seed-soaking liquid for corn doubling monoploids.Chemistry seed-soaking liquid prepared by the present invention can be used for carrying out chromosome artificial doubling to corn monoploid, plant is set to restore normal fertility, and then stable corn variety is obtained rapidly, pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl sulphoxide aqueous solution and trefanocide aqueous solution cooperate, there is good chromosome doubling effect to corn monoploid, simultaneously, since the toxicity of the raw material of use is smaller, effectively reduce the generation of lopsided seedling, it is handled by repeated ultrasonic and cooperates HIGH PRESSURE TREATMENT, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn, it has a vast market foreground.
Description
Technical field
The present invention relates to haploid breeding technical field, specifically a kind of chemical seed-soaking liquid for corn doubling monoploids
And preparation method thereof.
Background technique
Corn is annual tall and big draft, while being also the highest crops of whole world total output, to solve world food
Problem provides important support.Contain protein, fat, carbohydrate, calcium, phosphorus, iron, carrotene, vitamin B1, dimension in corn
Raw element B2, vitamin E, lysine, niacin, sitosterol, lecithin etc., since nutritional ingredient is more comprehensive, receive people
Widespread love, meanwhile, corn also has yield high, and quality is good, adaptable advantage.Corn is generally divided into Hard grain type, horse
Flute profile, saccharoid type, sweet tea powder type, bursting type, wax-matrix type, has bran type, half dent type, nine class at silty type.
With the continuous development of society, population pressure is also increasing, and then is also being continuously increased to the demand of corn.
In order to alleviate the pressure of food shortage, need to cultivate the corn variety of high yield and high quality.But breeding method conventional at present
The time usually expended is long, but also can consume a large amount of human and material resources and financial resources, and it is growing to be unable to satisfy people
Demand.Haploid Breeding of Maize method, can be rapidly and efficiently as the corn breeding method developed rapidly in recent years
The corn variety of high yield and high quality is cultivated on ground, and colchicine is that the currently used chemistry for corn doubling monoploids adds
Times reagent, still, due to using colchicine to be easy to produce lopsided seedling, it is, thus, sought for a kind of use for substituting colchicine
In the chemical seed-soaking liquid of corn doubling monoploids.
Summary of the invention
The chemical seed-soaking liquid and preparation method thereof that the purpose of the present invention is to provide a kind of for corn doubling monoploids, with
Solve the problems mentioned above in the background art.
To achieve the above object, the invention provides the following technical scheme:
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
15-45 parts, 8-36 parts of amiprophos-methyl aqueous solution, 15-55 parts of dimethyl sulphoxide aqueous solution, 8-30 parts of trefanocide aqueous solution.
As a further solution of the present invention: including below according to the raw material of parts by weight: 20-30 parts of sodium-chloride water solution,
10-20 parts of amiprophos-methyl aqueous solution, 20-40 parts of dimethyl sulphoxide aqueous solution, 10-20 parts of trefanocide aqueous solution.
As further scheme of the invention: including below according to the raw material of parts by weight: 25 parts of sodium-chloride water solution, first
12 parts of base amiprophos aqueous solution, 30 parts of dimethyl sulphoxide aqueous solution, 14 parts of trefanocide aqueous solution.
As further scheme of the invention: the concentration of the sodium-chloride water solution is 0.15mol/L；The methyl amine
The concentration of careless phosphorus aqueous solution is 18 μm of ol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The trefanocide aqueous solution
Concentration be 12 μm of ol/L.
As further scheme of the invention: the preparation method of the amiprophos-methyl aqueous solution is to weigh suitable first
Base amiprophos and deionized water carry out mechanical stirring 15min after mixing with the stirring rate of 150r/min, then carry out at ultrasound
Reason carries out 100 mesh filterings after standing 30min to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is
100W；The time of the ultrasonic treatment is 15min；By ultrasonic treatment, the mixed effect between material is effectively increased, in turn
Be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn.
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to mix with deionized water
It is even, ultrasonic disperse 10min is then carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C.
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
As further scheme of the invention: in step 1), the HIGH PRESSURE TREATMENT is to boost to material in 10s
120MPa, then pressure maintaining handles 10min at this pressure, then atmospheric pressure is depressurized in 10s, and cooperate in HIGH PRESSURE TREATMENT process
Treatment temperature is 28 DEG C；By HIGH PRESSURE TREATMENT, the mixed effect between material is effectively increased, and then is conducive to improve chemical leaching
Kind liquid is to the haploid chromosome doubling effect of corn.
The chemical seed-soaking liquid for corn doubling monoploids doubles the purposes in reagent in preparation monoploid chemistry.
Compared with prior art, the beneficial effects of the present invention are:
1) there are the chemical seed-soaking liquid prepared by the present invention for corn doubling monoploids good corn haploid chromosomes to add
Times effect can be used for carrying out chromosome artificial doubling to monoploid, so that plant is restored normal fertility, and then obtain stabilization rapidly
Corn variety；
2) present invention passes through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl sulphoxide aqueous solution and trefanocide aqueous solution
It cooperates, there is good chromosome doubling effect to corn monoploid, simultaneously as the toxicity of the raw material used is smaller,
Effectively reduce the generation of lopsided seedling；
3) present invention is handled by repeated ultrasonic, effectively increases material mixed effect, while cooperating HIGH PRESSURE TREATMENT, and then advantageous
In improving chemical seed-soaking liquid to the haploid chromosome doubling effect of corn, have a vast market foreground.
Specific embodiment
Technical solution of the present invention is described in more detail With reference to embodiment.
Embodiment 1
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
15 parts, 8 parts of amiprophos-methyl aqueous solution, 15 parts of dimethyl sulphoxide aqueous solution, 8 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 2
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
45 parts, 36 parts of amiprophos-methyl aqueous solution, 55 parts of dimethyl sulphoxide aqueous solution, 30 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 3
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
30 parts, 22 parts of amiprophos-methyl aqueous solution, 35 parts of dimethyl sulphoxide aqueous solution, 19 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 4
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
20 parts, 10 parts of amiprophos-methyl aqueous solution, 20 parts of dimethyl sulphoxide aqueous solution, 10 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 5
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
30 parts, 20 parts of amiprophos-methyl aqueous solution, 40 parts of dimethyl sulphoxide aqueous solution, 20 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 6
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
25 parts, 15 parts of amiprophos-methyl aqueous solution, 30 parts of dimethyl sulphoxide aqueous solution, 15 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Embodiment 7
A kind of chemical seed-soaking liquid for corn doubling monoploids, including below according to the raw material of parts by weight: sodium-chloride water solution
25 parts, 12 parts of amiprophos-methyl aqueous solution, 30 parts of dimethyl sulphoxide aqueous solution, 14 parts of trefanocide aqueous solution；
Wherein, the concentration of the sodium-chloride water solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μ
mol/L；The concentration of the dimethyl sulphoxide aqueous solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L；
The preparation method of the amiprophos-methyl aqueous solution be weigh suitable amiprophos-methyl and deionized water, after mixing with
The stirring rate of 150r/min carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh mistakes after standing 30min
Filter is to get the amiprophos-methyl aqueous solution；The power of the ultrasonic treatment is 100W；The time of the ultrasonic treatment is
15min；By ultrasonic treatment, the mixed effect between material is effectively increased, and then be conducive to improve chemical seed-soaking liquid to jade
The haploid chromosome doubling effect of rice；
The preparation method of the dimethyl sulphoxide aqueous solution is to weigh suitable dimethyl sulfoxide to be uniformly mixed with deionized water, so
Ultrasonic disperse 10min is carried out to get the dimethyl sulphoxide aqueous solution with the ultrasonic power of 30W at 25 DEG C afterwards.
In the present embodiment, the preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
The preparation method of the chemical seed-soaking liquid for corn doubling monoploids, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
Wherein, the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process；By HIGH PRESSURE TREATMENT, effectively mention
Mixed effect between high material, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get；Pass through sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl
Sulfoxide aqueous solution and trefanocide aqueous solution cooperate, and have good chromosome doubling effect to corn monoploid, meanwhile, by
It is smaller in the toxicity of the raw material of use, effectively reduce the generation of lopsided seedling.
Chemical seed-soaking liquid prepared by the present invention for corn doubling monoploids has good corn haploid chromosomes
Effect is doubled, can be used for carrying out chromosome artificial doubling to monoploid, plant is made to restore normal fertility, and then is obtained rapidly steady
Fixed corn variety；The present invention is happy by sodium-chloride water solution, amiprophos-methyl aqueous solution, dimethyl sulphoxide aqueous solution and fluorine
Lingshui Spring solution cooperates, and has good chromosome doubling effect to corn monoploid, simultaneously as the poison of the raw material used
Property is smaller, effectively reduces the generation of lopsided seedling；The present invention is handled by repeated ultrasonic, effectively increases material mixed effect,
Cooperate HIGH PRESSURE TREATMENT simultaneously, and then be conducive to improve chemical seed-soaking liquid to the haploid chromosome doubling effect of corn, has wide
Wealthy market prospects.
Better embodiment of the invention is explained in detail above, but the present invention is not limited to above-mentioned embodiment party
Formula within the knowledge of one of ordinary skill in the art can also be without departing from the purpose of the present invention
Various changes can be made.There is no necessity and possibility to exhaust all the enbodiments.And it thus amplifies out apparent
Variation or variation be still in the protection scope of this invention.
Claims (10)
1. a kind of chemical seed-soaking liquid for corn doubling monoploids, which is characterized in that including below according to the raw material of parts by weight:
15-45 parts of sodium-chloride water solution, 8-36 parts of amiprophos-methyl aqueous solution, 15-55 parts of dimethyl sulphoxide aqueous solution, trefanocide are water-soluble
8-30 parts of liquid.
2. the chemical seed-soaking liquid according to claim 1 for corn doubling monoploids, which is characterized in that including pressing below
According to the raw material of parts by weight: 20-30 parts of sodium-chloride water solution, 10-20 parts of amiprophos-methyl aqueous solution, dimethyl sulphoxide aqueous solution
20-40 parts, 10-20 parts of trefanocide aqueous solution.
3. the chemical seed-soaking liquid according to claim 2 for corn doubling monoploids, which is characterized in that including pressing below
According to the raw material of parts by weight: 25 parts of sodium-chloride water solution, 12 parts of amiprophos-methyl aqueous solution, 30 parts of dimethyl sulphoxide aqueous solution, fluorine
14 parts of happy Lingshui Spring solution.
4. the chemical seed-soaking liquid according to claim 1 for corn doubling monoploids, which is characterized in that the sodium chloride
The concentration of aqueous solution is 0.15mol/L；The concentration of the amiprophos-methyl aqueous solution is 18 μm of ol/L；The dimethyl sulfoxide water
The concentration of solution is 6g/L；The concentration of the trefanocide aqueous solution is 12 μm of ol/L.
5. the chemical seed-soaking liquid according to claim 4 for corn doubling monoploids, which is characterized in that the methyl amine
The preparation method of careless phosphorus aqueous solution is to weigh suitable amiprophos-methyl and deionized water, with the stirring speed of 150r/min after mixing
Rate carries out mechanical stirring 15min, is then ultrasonically treated, and carries out 100 mesh filterings after standing 30min to get the methyl amine
Careless phosphorus aqueous solution.
6. the chemical seed-soaking liquid according to claim 5 for corn doubling monoploids, which is characterized in that at the ultrasound
The power of reason is 100W；The time of the ultrasonic treatment is 15min.
7. the chemical seed-soaking liquid according to claim 4 for corn doubling monoploids, which is characterized in that the dimethyl
The preparation method of sulfoxide aqueous solution is to weigh suitable dimethyl sulfoxide and be uniformly mixed with deionized water, then at 25 DEG C with
The ultrasonic power of 30W carries out ultrasonic disperse 10min to get the dimethyl sulphoxide aqueous solution.
8. a kind of preparation method of the chemical seed-soaking liquid as claimed in claim 1 for corn doubling monoploids,
It is characterized in that, steps are as follows:
1) sodium-chloride water solution and dimethyl sulphoxide aqueous solution are weighed according to parts by weight, with the stirring rate of 100r/min after mixing
Mechanical stirring 20min is carried out, HIGH PRESSURE TREATMENT is then carried out, obtains mixture A；
2) amiprophos-methyl aqueous solution and trefanocide aqueous solution are weighed according to parts by weight, with the stirring rate of 140r/min after mixing
Mechanical stirring 15min is carried out, ultrasonic disperse 18min is then carried out with the ultrasonic power of 50W at 35 DEG C, obtains mixture B；
3) mixture A obtained in step 1) is uniformly mixed with mixture B obtained in step 2, with 60r/ at 25 DEG C
The stirring rate of min carry out mechanical stirring 20min to get.
9. the preparation method of the chemical seed-soaking liquid according to claim 8 for corn doubling monoploids, which is characterized in that
In step 1), the HIGH PRESSURE TREATMENT is that material is boosted to 120MPa in 10s, and then pressure maintaining handles 10min at this pressure,
Atmospheric pressure is depressurized in 10s again, and it is 28 DEG C that treatment temperature is cooperateed in HIGH PRESSURE TREATMENT process.
10. a kind of chemical seed-soaking liquid as claimed in claim 1 for corn doubling monoploids is in preparation monoploid
Chemistry doubles the purposes in reagent.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112056211A (en) * | 2020-08-11 | 2020-12-11 | 沈阳特亦佳玉米科技有限公司 | Method for creating giant burst corns |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195543A (en) * | 2007-12-18 | 2008-06-11 | 天津师范大学 | Phytase microbial inoculum fertilizer, producing method and application thereof |
| CN101518273A (en) * | 2009-03-19 | 2009-09-02 | 王基兴 | Biological compound pesticide and preparation method thereof |
| CN101869052A (en) * | 2010-05-05 | 2010-10-27 | 中国林业科学研究院热带林业研究所 | Method for building induction and cultivation system of alpine ash tetraploid plant |
| CN103053414A (en) * | 2011-10-24 | 2013-04-24 | 中国农业大学 | Method for doubling corn haploid by herbicide and special herbicide of method |
| CN103613447A (en) * | 2013-11-08 | 2014-03-05 | 苏州仁成生物科技有限公司 | Polyelement slow release granular fertilizer with high fertilizer efficiency and preparation method thereof |
| CN105660421A (en) * | 2016-03-15 | 2016-06-15 | 周常 | Woad triploid breeding method with obvious rhizome weight increase |
| CN106621879A (en) * | 2016-12-09 | 2017-05-10 | 陈彦羽 | High-pressure material mixing device for sugar industry |
| CN106665706A (en) * | 2016-12-06 | 2017-05-17 | 钦州市高新技术产业服务中心 | Corn seed-coating agent and preparation method thereof |
| CN106866309A (en) * | 2017-03-21 | 2017-06-20 | 蚌埠市兵凤赟种植农民专业合作社 | A kind of fuel for improving soil texture |
| CN107980620A (en) * | 2017-12-05 | 2018-05-04 | 沈阳农业大学 | A kind of the compound of plant chromosome doubles agent and method for doubling |
-
2018
- 2018-08-14 CN CN201810920405.5A patent/CN108935086A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101195543A (en) * | 2007-12-18 | 2008-06-11 | 天津师范大学 | Phytase microbial inoculum fertilizer, producing method and application thereof |
| CN101518273A (en) * | 2009-03-19 | 2009-09-02 | 王基兴 | Biological compound pesticide and preparation method thereof |
| CN101869052A (en) * | 2010-05-05 | 2010-10-27 | 中国林业科学研究院热带林业研究所 | Method for building induction and cultivation system of alpine ash tetraploid plant |
| CN103053414A (en) * | 2011-10-24 | 2013-04-24 | 中国农业大学 | Method for doubling corn haploid by herbicide and special herbicide of method |
| CN103613447A (en) * | 2013-11-08 | 2014-03-05 | 苏州仁成生物科技有限公司 | Polyelement slow release granular fertilizer with high fertilizer efficiency and preparation method thereof |
| CN105660421A (en) * | 2016-03-15 | 2016-06-15 | 周常 | Woad triploid breeding method with obvious rhizome weight increase |
| CN106665706A (en) * | 2016-12-06 | 2017-05-17 | 钦州市高新技术产业服务中心 | Corn seed-coating agent and preparation method thereof |
| CN106621879A (en) * | 2016-12-09 | 2017-05-10 | 陈彦羽 | High-pressure material mixing device for sugar industry |
| CN106866309A (en) * | 2017-03-21 | 2017-06-20 | 蚌埠市兵凤赟种植农民专业合作社 | A kind of fuel for improving soil texture |
| CN107980620A (en) * | 2017-12-05 | 2018-05-04 | 沈阳农业大学 | A kind of the compound of plant chromosome doubles agent and method for doubling |
Non-Patent Citations (3)
| Title |
|---|
| 张培风等: "玉米单倍体加倍技术的研究进展", 《山西农业科学》 * |
| 徐怀德: "《天然产物提取工艺学》", 30 June 2006, 中国轻工业出版社 * |
| 赵志峰: "《食品工艺学概论》", 31 December 2016, 四川大学出版社 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112056211A (en) * | 2020-08-11 | 2020-12-11 | 沈阳特亦佳玉米科技有限公司 | Method for creating giant burst corns |
| CN112056211B (en) * | 2020-08-11 | 2022-12-27 | 沈阳特亦佳玉米科技有限公司 | Method for creating giant burst corns |
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Application publication date: 20181207 |